AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029674.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029674.01.T01 MTR_5g090810 74.251 167 12 1 1 136 12 178 2.72e-78 244
MsG0580029674.01.T01 MTR_5g090810 73.377 154 40 1 106 259 319 471 7.74e-74 233
MsG0580029674.01.T01 MTR_5g090830 74.251 167 12 1 1 136 12 178 4.61e-78 243
MsG0580029674.01.T01 MTR_5g090830 72.727 154 41 1 106 259 319 471 7.82e-74 233
MsG0580029674.01.T01 MTR_5g090880 72.868 129 35 0 131 259 345 473 8.61e-63 204
MsG0580029674.01.T01 MTR_5g090880 64.072 167 28 2 1 135 12 178 9.15e-57 188
MsG0580029674.01.T01 MTR_3g450710 62.500 168 30 2 1 137 13 178 2.29e-53 179
MsG0580029674.01.T01 MTR_3g450710 69.369 111 32 1 147 257 360 468 2.60e-48 166
MsG0580029674.01.T01 MTR_3g450690 67.568 111 34 1 147 257 364 472 1.03e-48 167
MsG0580029674.01.T01 MTR_3g450690 56.977 172 38 2 1 136 13 184 7.53e-47 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029674.01.T01 AT1G78990 38.991 218 116 6 49 257 244 453 5.72e-40 144
MsG0580029674.01.T01 AT1G78990 49.697 165 49 3 2 135 16 177 1.59e-34 129
MsG0580029674.01.T01 AT1G32910 63.107 103 34 2 157 257 358 458 6.93e-40 144
MsG0580029674.01.T01 AT1G32910 53.333 165 37 4 4 135 18 175 4.81e-37 136
MsG0580029674.01.T01 AT5G16410 50.000 134 61 3 128 257 348 479 5.55e-37 136
MsG0580029674.01.T01 AT5G16410 45.087 173 53 5 2 138 39 205 2.63e-36 134

Find 83 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAGTCGAAGGTTGATTT+TGG 0.189640 5:-100647328 MsG0580029674.01.T01:CDS
AGCACACCATGTTTCTTCTT+TGG 0.268793 5:+100648442 None:intergenic
GTTTCTTATTATGCCTTTGC+TGG 0.282844 5:-100648606 MsG0580029674.01.T01:CDS
ACATGGTGTGCTTGCTGTTC+AGG 0.312822 5:-100648431 MsG0580029674.01.T01:intron
AAGAACACACCGACATCAAC+AGG 0.337593 5:+100648711 None:intergenic
TAAGGGACACTTGCACTTCA+TGG 0.343370 5:-100647188 MsG0580029674.01.T01:CDS
GGTGACAGAGGAGCATTTCT+TGG 0.346724 5:-100647466 MsG0580029674.01.T01:CDS
ATTTCTTGGGTTTAATAGAT+TGG 0.349608 5:-100647452 MsG0580029674.01.T01:CDS
GGAGAGCATCGTCCAGTTCC+TGG 0.353464 5:-100647427 MsG0580029674.01.T01:CDS
TGCCAATGCCAAGCCCTAAA+AGG 0.358606 5:-100647243 MsG0580029674.01.T01:CDS
TTTCCTTGGGGAGGTAGTGC+TGG 0.358995 5:-100647274 MsG0580029674.01.T01:CDS
AAGGAACATGTTTGCAGAAT+AGG 0.360809 5:+100648040 None:intergenic
ATTTCTTTAGACCAGTCTCT+TGG 0.380544 5:-100647150 MsG0580029674.01.T01:CDS
TATCATGGGCTGAGCCATTT+CGG 0.383782 5:+100648007 None:intergenic
TTTCTTGGGTTTAATAGATT+GGG 0.393187 5:-100647451 MsG0580029674.01.T01:CDS
CCAAAATCAACCTTCGACTC+TGG 0.403209 5:+100647328 None:intergenic
CAAACATGTTCCTTGTATCA+TGG 0.413069 5:-100648031 MsG0580029674.01.T01:CDS
TTTCTTTAGACCAGTCTCTT+GGG 0.423523 5:-100647149 MsG0580029674.01.T01:CDS
ATCCTTGCCAATCCAGGAAC+TGG 0.426912 5:+100647415 None:intergenic
CCTTGTATCATGGGCCGAAA+TGG 0.428090 5:-100648021 MsG0580029674.01.T01:CDS
CCAACATTTGTGGTATCTTC+AGG 0.429306 5:-100647361 MsG0580029674.01.T01:CDS
ATTAAATCAAGGTTTGATAG+TGG 0.431310 5:+100648741 None:intergenic
AATCCAGATGAAACTGTAGA+AGG 0.442984 5:-100648475 MsG0580029674.01.T01:intron
GTGACAGAGGAGCATTTCTT+GGG 0.453426 5:-100647465 MsG0580029674.01.T01:CDS
ATGAAGTGCAAGTGTCCCTT+AGG 0.455932 5:+100647190 None:intergenic
CGCTACCAATGCAAGAGCAT+TGG 0.468968 5:-100648767 MsG0580029674.01.T01:CDS
GCTCTACATGCACCTTCCTA+AGG 0.469205 5:-100647206 MsG0580029674.01.T01:CDS
GGATCAAAGACTTGTTATCA+TGG 0.470837 5:+100647992 None:intergenic
GTCTAAAGAAATGAGGAGCC+TGG 0.472034 5:+100647161 None:intergenic
TGGGTGCCAGTAGTAATTAT+AGG 0.473421 5:+100648675 None:intergenic
TATGACCAACGAGGAAATAG+TGG 0.494458 5:-100648796 MsG0580029674.01.T01:CDS
AGCTGCCACTATTTCCTCGT+TGG 0.494647 5:+100648791 None:intergenic
CAATAAATCCTTGCCAATCC+AGG 0.495236 5:+100647409 None:intergenic
GATGAAATGTGGTGGATTAG+TGG 0.498028 5:-100648096 MsG0580029674.01.T01:CDS
GGCACCCACCATAGCATTGT+GGG 0.501789 5:-100648660 MsG0580029674.01.T01:CDS
AGGATTTATTGTGGTAGCAC+TGG 0.506494 5:-100647397 MsG0580029674.01.T01:CDS
CTTGCACTTCATGGAGGCCC+AGG 0.516007 5:-100647179 MsG0580029674.01.T01:CDS
CCATTTCGGCCCATGATACA+AGG 0.516374 5:+100648021 None:intergenic
GCATCGTCCAGTTCCTGGAT+TGG 0.519656 5:-100647422 MsG0580029674.01.T01:CDS
TCAAACATCCCACAATGCTA+TGG 0.522391 5:+100648652 None:intergenic
GGTAGCACTGGTGGAGAAGA+AGG 0.530503 5:-100647385 MsG0580029674.01.T01:CDS
ATCCAGATGAAACTGTAGAA+GGG 0.535674 5:-100648474 MsG0580029674.01.T01:intron
ATTTATTGTGGTAGCACTGG+TGG 0.539802 5:-100647394 MsG0580029674.01.T01:CDS
AAACATGTTCCTTGTATCAT+GGG 0.539896 5:-100648030 MsG0580029674.01.T01:CDS
CTTGGGTTTAATAGATTGGG+TGG 0.541396 5:-100647448 MsG0580029674.01.T01:CDS
AGAAGAAGGACCAACATTTG+TGG 0.543509 5:-100647371 MsG0580029674.01.T01:CDS
TGAAGTCGTTGCTAATTCCA+TGG 0.543846 5:-100648583 MsG0580029674.01.T01:CDS
AATAAGCCTATAATTACTAC+TGG 0.553926 5:-100648681 MsG0580029674.01.T01:CDS
TTTGGAGGCGGCGGTGACAG+AGG 0.555868 5:-100647478 MsG0580029674.01.T01:intron
GCCCTAAAAGGAATGGTGAC+TGG 0.557544 5:-100647231 MsG0580029674.01.T01:CDS
ACACAACACAATATTAATTG+AGG 0.558055 5:+100647119 None:intergenic
AGGAGGTAATCCCAAGAGAC+TGG 0.562245 5:+100647139 None:intergenic
AACATCCCACAATGCTATGG+TGG 0.566613 5:+100648655 None:intergenic
TCTAAAGAAATGAGGAGCCT+GGG 0.578551 5:+100647162 None:intergenic
CCTGAAGATACCACAAATGT+TGG 0.579526 5:+100647361 None:intergenic
GGAAGCCAATGCTCTTGCAT+TGG 0.585364 5:+100648762 None:intergenic
ACAAAGGTTTCCAGAGTCGA+AGG 0.585762 5:-100647338 MsG0580029674.01.T01:CDS
GATCAAAGACTTGTTATCAT+GGG 0.588501 5:+100647993 None:intergenic
AGCAACGACTTCACCAGCAA+AGG 0.588840 5:+100648593 None:intergenic
TTGTGGTATCTTCAGGACAA+AGG 0.593564 5:-100647354 MsG0580029674.01.T01:CDS
ATGCCAAGCCCTAAAAGGAA+TGG 0.595010 5:-100647238 MsG0580029674.01.T01:CDS
CTCTACATGCACCTTCCTAA+GGG 0.601014 5:-100647205 MsG0580029674.01.T01:CDS
AGTGCAAGTGTCCCTTAGGA+AGG 0.602305 5:+100647194 None:intergenic
GTCCAGTTCCTGGATTGGCA+AGG 0.607030 5:-100647417 MsG0580029674.01.T01:CDS
TTGTTCTTGTGCAGACAACA+TGG 0.607336 5:-100648118 MsG0580029674.01.T01:intron
GAGACTGGTCTAAAGAAATG+AGG 0.612013 5:+100647154 None:intergenic
AGAACACACCGACATCAACA+GGG 0.612828 5:+100648712 None:intergenic
CTTGTTCCAAAGAAGAAACA+TGG 0.620389 5:-100648448 MsG0580029674.01.T01:CDS
TGGCACCCACCATAGCATTG+TGG 0.626128 5:-100648661 MsG0580029674.01.T01:CDS
ACATCCCACAATGCTATGGT+GGG 0.631579 5:+100648656 None:intergenic
CAACACAATATTAATTGAGG+AGG 0.635825 5:+100647122 None:intergenic
GAAGTCGTTGCTAATTCCAT+GGG 0.644829 5:-100648582 MsG0580029674.01.T01:intron
GAACACACCGACATCAACAG+GGG 0.646980 5:+100648713 None:intergenic
GGATTGGCAAGGATTTATTG+TGG 0.648587 5:-100647406 MsG0580029674.01.T01:CDS
AAATGATAAGATCCAAACAC+AGG 0.657847 5:+100647295 None:intergenic
ACAACATGGATGAAATGTGG+TGG 0.668180 5:-100648104 MsG0580029674.01.T01:CDS
AATAGGCATCCGCTATACGA+TGG 0.674136 5:+100648057 None:intergenic
GAAATGTGGTGGATTAGTGG+TGG 0.677628 5:-100648093 MsG0580029674.01.T01:CDS
AACACACCGACATCAACAGG+GGG 0.687180 5:+100648714 None:intergenic
CAGACAACATGGATGAAATG+TGG 0.692012 5:-100648107 MsG0580029674.01.T01:CDS
TAGCCAGCACTACCTCCCCA+AGG 0.698597 5:+100647271 None:intergenic
GGGACACTTGCACTTCATGG+AGG 0.714604 5:-100647185 MsG0580029674.01.T01:CDS
CAACGTGAATATGACCAACG+AGG 0.763069 5:-100648805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAAATTCTTTAGCTA+AGG - Chr5:100647293-100647312 MsG0580029674.01.T01:CDS 20.0%
!!! AATTTTAATTTTGAACCACT+TGG - Chr5:100647718-100647737 MsG0580029674.01.T01:intron 20.0%
!!! CGATTATGAATTCATATTTT+GGG - Chr5:100648337-100648356 MsG0580029674.01.T01:intron 20.0%
! AAAACATTGTTGCAAAAATG+GGG - Chr5:100648264-100648283 MsG0580029674.01.T01:intron 25.0%
! AATAAGCCTATAATTACTAC+TGG - Chr5:100647246-100647265 MsG0580029674.01.T01:CDS 25.0%
! AGTAAAATGATAAGACCAAT+GGG + Chr5:100647659-100647678 None:intergenic 25.0%
! ATAGTATATGCGACTTATTA+TGG + Chr5:100648108-100648127 None:intergenic 25.0%
! ATTAAATCAAGGTTTGATAG+TGG + Chr5:100647189-100647208 None:intergenic 25.0%
! ATTTCTTGGGTTTAATAGAT+TGG - Chr5:100648475-100648494 MsG0580029674.01.T01:intron 25.0%
! GAAAACATTGTTGCAAAAAT+GGG - Chr5:100648263-100648282 MsG0580029674.01.T01:intron 25.0%
! TAGTATATGCGACTTATTAT+GGG + Chr5:100648107-100648126 None:intergenic 25.0%
! TATAATCTATACCATATGCA+AGG + Chr5:100647772-100647791 None:intergenic 25.0%
! TGAAAACATTGTTGCAAAAA+TGG - Chr5:100648262-100648281 MsG0580029674.01.T01:intron 25.0%
! TTAATATCAGAGTCAAATTC+AGG + Chr5:100648077-100648096 None:intergenic 25.0%
! TTTCTTGGGTTTAATAGATT+GGG - Chr5:100648476-100648495 MsG0580029674.01.T01:intron 25.0%
!! GCGATTATGAATTCATATTT+TGG - Chr5:100648336-100648355 MsG0580029674.01.T01:intron 25.0%
AAACATGTTCCTTGTATCAT+GGG - Chr5:100647897-100647916 MsG0580029674.01.T01:intron 30.0%
AAATGATAAGATCCAAACAC+AGG + Chr5:100648635-100648654 None:intergenic 30.0%
ACATACATGTCATAAAGTGA+AGG + Chr5:100648023-100648042 None:intergenic 30.0%
AGTATATGCGACTTATTATG+GGG + Chr5:100648106-100648125 None:intergenic 30.0%
ATTAGTACTTGTGTTTAGAC+AGG - Chr5:100647624-100647643 MsG0580029674.01.T01:intron 30.0%
CAGTAAAATGATAAGACCAA+TGG + Chr5:100647660-100647679 None:intergenic 30.0%
TTTGTTAATCTACCACCATA+GGG + Chr5:100648378-100648397 None:intergenic 30.0%
! ACCAAAACATTTTGTTCTCA+AGG - Chr5:100647540-100647559 MsG0580029674.01.T01:intron 30.0%
! CCAAAACATTTTGTTCTCAA+GGG - Chr5:100647541-100647560 MsG0580029674.01.T01:intron 30.0%
! TGGATCTTATCATTTTCCTT+GGG - Chr5:100648640-100648659 MsG0580029674.01.T01:CDS 30.0%
! TTAATTTTGAACCACTTGGT+TGG - Chr5:100647722-100647741 MsG0580029674.01.T01:intron 30.0%
! TTGGATCTTATCATTTTCCT+TGG - Chr5:100648639-100648658 MsG0580029674.01.T01:CDS 30.0%
! TTTTGTTAATCTACCACCAT+AGG + Chr5:100648379-100648398 None:intergenic 30.0%
!! GATCAAAGACTTGTTATCAT+GGG + Chr5:100647937-100647956 None:intergenic 30.0%
!! GTTTTCATTATCGATTGAAG+CGG + Chr5:100648249-100648268 None:intergenic 30.0%
AAGGAACATGTTTGCAGAAT+AGG + Chr5:100647890-100647909 None:intergenic 35.0%
AATCCAGATGAAACTGTAGA+AGG - Chr5:100647452-100647471 MsG0580029674.01.T01:CDS 35.0%
AATCTATACCATATGCAAGG+TGG + Chr5:100647769-100647788 None:intergenic 35.0%
AATGATAAGACCAATGGGTA+CGG + Chr5:100647654-100647673 None:intergenic 35.0%
ACATGTCATAAAGTGAAGGA+TGG + Chr5:100648019-100648038 None:intergenic 35.0%
ATCCAGATGAAACTGTAGAA+GGG - Chr5:100647453-100647472 MsG0580029674.01.T01:CDS 35.0%
ATTTCTTTAGACCAGTCTCT+TGG - Chr5:100648777-100648796 MsG0580029674.01.T01:CDS 35.0%
CAAACATGTTCCTTGTATCA+TGG - Chr5:100647896-100647915 MsG0580029674.01.T01:intron 35.0%
CTTGTTCCAAAGAAGAAACA+TGG - Chr5:100647479-100647498 MsG0580029674.01.T01:intron 35.0%
GTTTCTTATTATGCCTTTGC+TGG - Chr5:100647321-100647340 MsG0580029674.01.T01:CDS 35.0%
TCTCAAGGGAATATTCGAAT+AGG - Chr5:100647555-100647574 MsG0580029674.01.T01:intron 35.0%
TGGTAGATTAACAAAAGAGC+TGG - Chr5:100648383-100648402 MsG0580029674.01.T01:intron 35.0%
TTCTGCTTTCTTGTGGAAAA+TGG - Chr5:100648214-100648233 MsG0580029674.01.T01:intron 35.0%
TTTCCACAAGAAAGCAGAAA+AGG + Chr5:100648213-100648232 None:intergenic 35.0%
TTTCTTGTGGAAAATGGTTG+CGG - Chr5:100648220-100648239 MsG0580029674.01.T01:intron 35.0%
TTTCTTTAGACCAGTCTCTT+GGG - Chr5:100648778-100648797 MsG0580029674.01.T01:CDS 35.0%
! AGATCGAGTTCATGAGTTTT+TGG - Chr5:100648431-100648450 MsG0580029674.01.T01:intron 35.0%
! CCCTTGAGAACAAAATGTTT+TGG + Chr5:100647544-100647563 None:intergenic 35.0%
! GGATCTTATCATTTTCCTTG+GGG - Chr5:100648641-100648660 MsG0580029674.01.T01:CDS 35.0%
!! GGATCAAAGACTTGTTATCA+TGG + Chr5:100647938-100647957 None:intergenic 35.0%
!!! TGGTTTAGTGGTGTTGTTTT+GGG - Chr5:100647742-100647761 MsG0580029674.01.T01:intron 35.0%
!!! TTGGTTTAGTGGTGTTGTTT+TGG - Chr5:100647741-100647760 MsG0580029674.01.T01:intron 35.0%
AATGTGCTTTCGATTCCCTA+TGG - Chr5:100648360-100648379 MsG0580029674.01.T01:intron 40.0%
ACAACATGGATGAAATGTGG+TGG - Chr5:100647823-100647842 MsG0580029674.01.T01:intron 40.0%
ACTACCACCATTGTTTGAGT+TGG + Chr5:100648168-100648187 None:intergenic 40.0%
AGAAGAAGGACCAACATTTG+TGG - Chr5:100648556-100648575 MsG0580029674.01.T01:intron 40.0%
AGAAGTGACTGTCGAAAACA+AGG + Chr5:100647972-100647991 None:intergenic 40.0%
AGCACACCATGTTTCTTCTT+TGG + Chr5:100647488-100647507 None:intergenic 40.0%
AGGATTTATTGTGGTAGCAC+TGG - Chr5:100648530-100648549 MsG0580029674.01.T01:intron 40.0%
CAATAAATCCTTGCCAATCC+AGG + Chr5:100648521-100648540 None:intergenic 40.0%
CAGACAACATGGATGAAATG+TGG - Chr5:100647820-100647839 MsG0580029674.01.T01:intron 40.0%
CAGGGGGAAGAATTAAATCA+AGG + Chr5:100647200-100647219 None:intergenic 40.0%
CCAACATTTGTGGTATCTTC+AGG - Chr5:100648566-100648585 MsG0580029674.01.T01:intron 40.0%
CCTGAAGATACCACAAATGT+TGG + Chr5:100648569-100648588 None:intergenic 40.0%
CGATTGTTGCAAAGTAACTC+AGG + Chr5:100647374-100647393 None:intergenic 40.0%
CTTGGGTTTAATAGATTGGG+TGG - Chr5:100648479-100648498 MsG0580029674.01.T01:intron 40.0%
GAAAACAAGGTTGCGTTGTT+GGG + Chr5:100647959-100647978 None:intergenic 40.0%
GAAGTCGTTGCTAATTCCAT+GGG - Chr5:100647345-100647364 MsG0580029674.01.T01:CDS 40.0%
GTCATAAAGTGAAGGATGGA+TGG + Chr5:100648015-100648034 None:intergenic 40.0%
TAAAGTGAAGGATGGATGGA+AGG + Chr5:100648011-100648030 None:intergenic 40.0%
TCAAACATCCCACAATGCTA+TGG + Chr5:100647278-100647297 None:intergenic 40.0%
TCACTAGCCAACTCAAACAA+TGG - Chr5:100648158-100648177 MsG0580029674.01.T01:intron 40.0%
TCTAAAGAAATGAGGAGCCT+GGG + Chr5:100648768-100648787 None:intergenic 40.0%
TGAAGTCGTTGCTAATTCCA+TGG - Chr5:100647344-100647363 MsG0580029674.01.T01:CDS 40.0%
TGGGTGCCAGTAGTAATTAT+AGG + Chr5:100647255-100647274 None:intergenic 40.0%
TTCCCTTCTACAGTTTCATC+TGG + Chr5:100647458-100647477 None:intergenic 40.0%
TTGTGGTATCTTCAGGACAA+AGG - Chr5:100648573-100648592 MsG0580029674.01.T01:intron 40.0%
TTGTTCTTGTGCAGACAACA+TGG - Chr5:100647809-100647828 MsG0580029674.01.T01:intron 40.0%
! AACCAGTCACCATTCCTTTT+AGG + Chr5:100648701-100648720 None:intergenic 40.0%
! ACCAGTCACCATTCCTTTTA+GGG + Chr5:100648700-100648719 None:intergenic 40.0%
! AGTCCTTTTCTGCTTTCTTG+TGG - Chr5:100648207-100648226 MsG0580029674.01.T01:intron 40.0%
! ATTTATTGTGGTAGCACTGG+TGG - Chr5:100648533-100648552 MsG0580029674.01.T01:intron 40.0%
! CACTTTTGACCATCGTATAG+CGG - Chr5:100647861-100647880 MsG0580029674.01.T01:intron 40.0%
! GAGTTACTTTGCAACAATCG+TGG - Chr5:100647374-100647393 MsG0580029674.01.T01:CDS 40.0%
! GGATTGGCAAGGATTTATTG+TGG - Chr5:100648521-100648540 MsG0580029674.01.T01:intron 40.0%
! TCAGAGTCAAATTCAGGTTC+AGG + Chr5:100648071-100648090 None:intergenic 40.0%
! TCTTATCATTTTCCTTGGGG+AGG - Chr5:100648644-100648663 MsG0580029674.01.T01:CDS 40.0%
!! AGTCGAAGGTTGATTTTGGA+TGG - Chr5:100648603-100648622 MsG0580029674.01.T01:CDS 40.0%
!! AGTTCATGAGTTTTTGGAGG+CGG - Chr5:100648437-100648456 MsG0580029674.01.T01:CDS 40.0%
!! GAGACTGGTCTAAAGAAATG+AGG + Chr5:100648776-100648795 None:intergenic 40.0%
!! GATGAAATGTGGTGGATTAG+TGG - Chr5:100647831-100647850 MsG0580029674.01.T01:intron 40.0%
!! GGTTGATAATCCGCTATGTT+GGG - Chr5:100648404-100648423 MsG0580029674.01.T01:intron 40.0%
!! GTCGAAGGTTGATTTTGGAT+GGG - Chr5:100648604-100648623 MsG0580029674.01.T01:CDS 40.0%
!! TCGAAGGTTGATTTTGGATG+GGG - Chr5:100648605-100648624 MsG0580029674.01.T01:CDS 40.0%
!! TCGAGTTCATGAGTTTTTGG+AGG - Chr5:100648434-100648453 MsG0580029674.01.T01:CDS 40.0%
!! TGGTTGATAATCCGCTATGT+TGG - Chr5:100648403-100648422 MsG0580029674.01.T01:intron 40.0%
AAAATGGGGCTTGTGGTTGA+CGG - Chr5:100648278-100648297 MsG0580029674.01.T01:intron 45.0%
AAAGTAACTCAGGTCACCCA+TGG + Chr5:100647364-100647383 None:intergenic 45.0%
AACATCCCACAATGCTATGG+TGG + Chr5:100647275-100647294 None:intergenic 45.0%
AAGAACACACCGACATCAAC+AGG + Chr5:100647219-100647238 None:intergenic 45.0%
AATAGGCATCCGCTATACGA+TGG + Chr5:100647873-100647892 None:intergenic 45.0%
ACAAAGGTTTCCAGAGTCGA+AGG - Chr5:100648589-100648608 MsG0580029674.01.T01:CDS 45.0%
ACACCACTAAACCAACCAAG+TGG + Chr5:100647736-100647755 None:intergenic 45.0%
ACATCCCACAATGCTATGGT+GGG + Chr5:100647274-100647293 None:intergenic 45.0%
ACTTTGCAACAATCGTGGAG+TGG - Chr5:100647379-100647398 MsG0580029674.01.T01:CDS 45.0%
AGAACACACCGACATCAACA+GGG + Chr5:100647218-100647237 None:intergenic 45.0%
ATGAAGTGCAAGTGTCCCTT+AGG + Chr5:100648740-100648759 None:intergenic 45.0%
ATGCCAAGCCCTAAAAGGAA+TGG - Chr5:100648689-100648708 MsG0580029674.01.T01:CDS 45.0%
ATTCTTCCCCCTGTTGATGT+CGG - Chr5:100647207-100647226 MsG0580029674.01.T01:CDS 45.0%
CCAAAATCAACCTTCGACTC+TGG + Chr5:100648602-100648621 None:intergenic 45.0%
CGAAAACAAGGTTGCGTTGT+TGG + Chr5:100647960-100647979 None:intergenic 45.0%
CTAGCCAACTCAAACAATGG+TGG - Chr5:100648161-100648180 MsG0580029674.01.T01:intron 45.0%
CTCTACATGCACCTTCCTAA+GGG - Chr5:100648722-100648741 MsG0580029674.01.T01:CDS 45.0%
GTCTAAAGAAATGAGGAGCC+TGG + Chr5:100648769-100648788 None:intergenic 45.0%
TAAGGGACACTTGCACTTCA+TGG - Chr5:100648739-100648758 MsG0580029674.01.T01:CDS 45.0%
TGATAATCCGCTATGTTGGG+TGG - Chr5:100648407-100648426 MsG0580029674.01.T01:intron 45.0%
TGTTGCAAAAATGGGGCTTG+TGG - Chr5:100648271-100648290 MsG0580029674.01.T01:intron 45.0%
TTGTGGTTGACGGAAGAAAG+AGG - Chr5:100648288-100648307 MsG0580029674.01.T01:intron 45.0%
! GAGTCAAATTCAGGTTCAGG+TGG + Chr5:100648068-100648087 None:intergenic 45.0%
! TATCATGGGCTGAGCCATTT+CGG + Chr5:100647923-100647942 None:intergenic 45.0%
! TCAAATTCAGGTTCAGGTGG+TGG + Chr5:100648065-100648084 None:intergenic 45.0%
!! CCAGAGTCGAAGGTTGATTT+TGG - Chr5:100648599-100648618 MsG0580029674.01.T01:CDS 45.0%
!! CGAAGGTTGATTTTGGATGG+GGG - Chr5:100648606-100648625 MsG0580029674.01.T01:CDS 45.0%
!! GAAATGTGGTGGATTAGTGG+TGG - Chr5:100647834-100647853 MsG0580029674.01.T01:intron 45.0%
!! GAACCACTTGGTTGGTTTAG+TGG - Chr5:100647730-100647749 MsG0580029674.01.T01:intron 45.0%
!! GTGACAGAGGAGCATTTCTT+GGG - Chr5:100648462-100648481 MsG0580029674.01.T01:CDS 45.0%
!!! TCACCATTCCTTTTAGGGCT+TGG + Chr5:100648695-100648714 None:intergenic 45.0%
!!! TTCCTTTTAGGGCTTGGCAT+TGG + Chr5:100648689-100648708 None:intergenic 45.0%
!!! TTTTGGGACCACCTTGCATA+TGG - Chr5:100647758-100647777 MsG0580029674.01.T01:intron 45.0%
AACACACCGACATCAACAGG+GGG + Chr5:100647216-100647235 None:intergenic 50.0%
AGCAACGACTTCACCAGCAA+AGG + Chr5:100647337-100647356 None:intergenic 50.0%
AGCTGCCACTATTTCCTCGT+TGG + Chr5:100647139-100647158 None:intergenic 50.0%
AGGAGGTAATCCCAAGAGAC+TGG + Chr5:100648791-100648810 None:intergenic 50.0%
AGTGAAGGATGGATGGAAGG+TGG + Chr5:100648008-100648027 None:intergenic 50.0%
AGTGCAAGTGTCCCTTAGGA+AGG + Chr5:100648736-100648755 None:intergenic 50.0%
ATCCTTGCCAATCCAGGAAC+TGG + Chr5:100648515-100648534 None:intergenic 50.0%
CCATTTCGGCCCATGATACA+AGG + Chr5:100647909-100647928 None:intergenic 50.0%
CCTTGTATCATGGGCCGAAA+TGG - Chr5:100647906-100647925 MsG0580029674.01.T01:intron 50.0%
CGGAAGAAAGAGGCTAAGCA+CGG - Chr5:100648298-100648317 MsG0580029674.01.T01:intron 50.0%
GAACACACCGACATCAACAG+GGG + Chr5:100647217-100647236 None:intergenic 50.0%
GCCCTAAAAGGAATGGTGAC+TGG - Chr5:100648696-100648715 MsG0580029674.01.T01:CDS 50.0%
GCTCTACATGCACCTTCCTA+AGG - Chr5:100648721-100648740 MsG0580029674.01.T01:CDS 50.0%
GGAAGCCAATGCTCTTGCAT+TGG + Chr5:100647168-100647187 None:intergenic 50.0%
GTGAAGGATGGATGGAAGGT+GGG + Chr5:100648007-100648026 None:intergenic 50.0%
GTGCTTTCGATTCCCTATGG+TGG - Chr5:100648363-100648382 MsG0580029674.01.T01:intron 50.0%
TGCCAATGCCAAGCCCTAAA+AGG - Chr5:100648684-100648703 MsG0580029674.01.T01:CDS 50.0%
TGGTGGAGGTAGATCAGAGA+CGG + Chr5:100648048-100648067 None:intergenic 50.0%
! AATTCAGGTTCAGGTGGTGG+AGG + Chr5:100648062-100648081 None:intergenic 50.0%
! CGCTACCAATGCAAGAGCAT+TGG - Chr5:100647160-100647179 MsG0580029674.01.T01:CDS 50.0%
!! ACATGGTGTGCTTGCTGTTC+AGG - Chr5:100647496-100647515 MsG0580029674.01.T01:intron 50.0%
!! GGTGACAGAGGAGCATTTCT+TGG - Chr5:100648461-100648480 MsG0580029674.01.T01:CDS 50.0%
!! TCATGAGTTTTTGGAGGCGG+CGG - Chr5:100648440-100648459 MsG0580029674.01.T01:CDS 50.0%
CGATCTGCCACCCAACATAG+CGG + Chr5:100648417-100648436 None:intergenic 55.0%
GCATCGTCCAGTTCCTGGAT+TGG - Chr5:100648505-100648524 MsG0580029674.01.T01:intron 55.0%
GGCACCCACCATAGCATTGT+GGG - Chr5:100647267-100647286 MsG0580029674.01.T01:CDS 55.0%
GGGACACTTGCACTTCATGG+AGG - Chr5:100648742-100648761 MsG0580029674.01.T01:CDS 55.0%
GGTGGAGGTAGATCAGAGAC+GGG + Chr5:100648047-100648066 None:intergenic 55.0%
GTGGAGGTAGATCAGAGACG+GGG + Chr5:100648046-100648065 None:intergenic 55.0%
TGGCACCCACCATAGCATTG+TGG - Chr5:100647266-100647285 MsG0580029674.01.T01:CDS 55.0%
TTTCCTTGGGGAGGTAGTGC+TGG - Chr5:100648653-100648672 MsG0580029674.01.T01:CDS 55.0%
!! GGTAGCACTGGTGGAGAAGA+AGG - Chr5:100648542-100648561 MsG0580029674.01.T01:intron 55.0%
!! GTCCAGTTCCTGGATTGGCA+AGG - Chr5:100648510-100648529 MsG0580029674.01.T01:intron 55.0%
!!! GGACTCGAGTTTTGAGCGCT+TGG + Chr5:100648192-100648211 None:intergenic 55.0%
CTTGCACTTCATGGAGGCCC+AGG - Chr5:100648748-100648767 MsG0580029674.01.T01:CDS 60.0%
GACAGGCACTCCGTACCCAT+TGG - Chr5:100647641-100647660 MsG0580029674.01.T01:intron 60.0%
GGATGGGGGAAGCCTGTGTT+TGG - Chr5:100648620-100648639 MsG0580029674.01.T01:CDS 60.0%
TAGCCAGCACTACCTCCCCA+AGG + Chr5:100648659-100648678 None:intergenic 60.0%
!! GGAGAGCATCGTCCAGTTCC+TGG - Chr5:100648500-100648519 MsG0580029674.01.T01:intron 60.0%
TTTGGAGGCGGCGGTGACAG+AGG - Chr5:100648449-100648468 MsG0580029674.01.T01:CDS 65.0%
! GATGGAAGGTGGGCGTCGTG+GGG + Chr5:100647997-100648016 None:intergenic 65.0%
! GGATGGAAGGTGGGCGTCGT+GGG + Chr5:100647998-100648017 None:intergenic 65.0%
! TGGATGGAAGGTGGGCGTCG+TGG + Chr5:100647999-100648018 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 100647132 100648817 100647132 ID=MsG0580029674.01;Name=MsG0580029674.01
Chr5 mRNA 100647132 100648817 100647132 ID=MsG0580029674.01.T01;Parent=MsG0580029674.01;Name=MsG0580029674.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0.33|0|1|1|1|4|0|259
Chr5 exon 100648583 100648817 100648583 ID=MsG0580029674.01.T01:exon:36577;Parent=MsG0580029674.01.T01
Chr5 exon 100648432 100648490 100648432 ID=MsG0580029674.01.T01:exon:36576;Parent=MsG0580029674.01.T01
Chr5 exon 100648003 100648126 100648003 ID=MsG0580029674.01.T01:exon:36575;Parent=MsG0580029674.01.T01
Chr5 exon 100647132 100647493 100647132 ID=MsG0580029674.01.T01:exon:36574;Parent=MsG0580029674.01.T01
Chr5 CDS 100648583 100648817 100648583 ID=MsG0580029674.01.T01:cds;Parent=MsG0580029674.01.T01
Chr5 CDS 100648432 100648490 100648432 ID=MsG0580029674.01.T01:cds;Parent=MsG0580029674.01.T01
Chr5 CDS 100648003 100648126 100648003 ID=MsG0580029674.01.T01:cds;Parent=MsG0580029674.01.T01
Chr5 CDS 100647132 100647493 100647132 ID=MsG0580029674.01.T01:cds;Parent=MsG0580029674.01.T01
Gene Sequence

>MsG0580029674.01.T01

ATGACCAACGAGGAAATAGTGGCAGCTGCGCTACCAATGCAAGAGCATTGGCTTCCACTATCAAACCTTGATTTAATTCTTCCCCCTGTTGATGTCGGTGTGTTCTTTTGCTACAATAAGCCTATAATTACTACTGGCACCCACCATAGCATTGTGGGATGTTTGAAAAATTCTTTAGCTAAGGCTCTTGTTTCTTATTATGCCTTTGCTGGTGAAGTCGTTGCTAATTCCATGGATGAAACTGTAGAAGGGAAGCTTGTTCCAAAGAAGAAACATGGTGTGCTTGCTGTTCAGACAACATGGATGAAATGTGGTGGATTAGTGGTGGCTTGCACTTTTGACCATCGTATAGCGGATGCCTATTCTGCAAACATGTTCCTTGTATCATGGGCCGAAATGGCTCAGCCCATGATAACAAGCGGCGGTGACAGAGGAGCATTTCTTGGGTTTAATAGATTGGGTGGAGAGCATCGTCCAGTTCCTGGATTGGCAAGGATTTATTGTGGTAGCACTGGTGGAGAAGAAGGACCAACATTTGTGGTATCTTCAGGACAAAGGTTTCCAGAGTCGAAGGTTGATTTTGGATGGGGGAAGCCTGTGTTTGGATCTTATCATTTTCCTTGGGGAGGTAGTGCTGGCTATGTTATGCCAATGCCAAGCCCTAAAAGGAATGGTGACTGGTTGCTCTACATGCACCTTCCTAAGGGACACTTGCACTTCATGGAGGCCCAGGCTCCTCATTTCTTTAGACCAGTCTCTTGGGATTACCTCCTCAATTAA
Protein sequence

>MsG0580029674.01.T01

MTNEEIVAAALPMQEHWLPLSNLDLILPPVDVGVFFCYNKPIITTGTHHSIVGCLKNSLAKALVSYYAFAGEVVANSMDETVEGKLVPKKKHGVLAVQTTWMKCGGLVVACTFDHRIADAYSANMFLVSWAEMAQPMITSGGDRGAFLGFNRLGGEHRPVPGLARIYCGSTGGEEGPTFVVSSGQRFPESKVDFGWGKPVFGSYHFPWGGSAGYVMPMPSPKRNGDWLLYMHLPKGHLHFMEAQAPHFFRPVSWDYLLN*