AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016299.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016299.01.T01 MTR_3g087270 93.970 199 12 0 1 199 1 199 3.94e-141 392
MsG0380016299.01.T01 MTR_3g087278 89.447 199 21 0 1 199 1 199 5.02e-132 369
MsG0380016299.01.T01 MTR_3g087280 87.940 199 24 0 1 199 1 199 8.59e-132 368
MsG0380016299.01.T01 MTR_3g087330 84.925 199 30 0 1 199 1 199 7.56e-128 358
MsG0380016299.01.T01 MTR_3g087325 85.930 199 28 0 1 199 1 199 3.75e-126 354
MsG0380016299.01.T01 MTR_3g087275 85.930 199 27 1 1 199 1 198 1.27e-123 347
MsG0380016299.01.T01 MTR_3g087300 65.990 197 23 3 1 197 1 153 4.05e-86 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016299.01.T01 AT5G65030 30.964 197 124 3 11 199 10 202 6.12e-26 99.4
MsG0380016299.01.T01 AT5G10210 32.124 193 109 7 15 196 13 194 6.00e-24 94.4

Find 35 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACCAAATCAGTACCATTT+TGG 0.165786 3:-81664965 MsG0380016299.01.T01:CDS
ATAAGAGTAGGTTGAATAAT+TGG 0.258714 3:-81664650 MsG0380016299.01.T01:CDS
TGCGCTTGGCGCAGCTTTGG+AGG 0.349292 3:-81664559 MsG0380016299.01.T01:CDS
TTAATCTCCACTTCTACTTC+CGG 0.353807 3:+81664711 None:intergenic
TTAAATGACTCGTCCCAAAA+TGG 0.387620 3:+81664951 None:intergenic
ATTTGCGCTTGGCGCAGCTT+TGG 0.402289 3:-81664562 MsG0380016299.01.T01:CDS
GCTCCAACACTAGGCTTTGT+TGG 0.406615 3:+81664886 None:intergenic
AGTGGCTACGGTTCATGAAA+TGG 0.409468 3:-81664682 MsG0380016299.01.T01:CDS
GTCCCAAAATGGTACTGATT+TGG 0.410434 3:+81664962 None:intergenic
GATTCTAGAATCACTTTCCA+TGG 0.440184 3:+81664813 None:intergenic
GCAAGGCTGTATCTTCCAAC+AGG 0.447411 3:-81665026 MsG0380016299.01.T01:CDS
TGCACCAAGCGCGATGATCA+TGG 0.461361 3:+81664604 None:intergenic
TTGGAAAAGGTGAGATTCCA+TGG 0.470388 3:-81664830 MsG0380016299.01.T01:CDS
AAAGCCTAGTGTTGGAGCTA+AGG 0.499139 3:-81664881 MsG0380016299.01.T01:CDS
TTTGCCTTAGCTCCAACACT+AGG 0.507447 3:+81664877 None:intergenic
CAACTGAAGTTTGCATATGC+AGG 0.509989 3:+81665110 None:intergenic
GGGTCACAACTTATTGGAAA+AGG 0.536727 3:-81664843 MsG0380016299.01.T01:CDS
AGTGGAGATTAAAATAAGAG+TGG 0.548596 3:-81664700 MsG0380016299.01.T01:CDS
AATGAGTGTGGATGCAAGAA+TGG 0.549203 3:-81664627 MsG0380016299.01.T01:CDS
ATGGCCATGATCATCGCGCT+TGG 0.550412 3:-81664608 MsG0380016299.01.T01:CDS
GATTAAAATAAGAGTGGCTA+CGG 0.551977 3:-81664694 MsG0380016299.01.T01:CDS
GCTAAGGCAAAGGAAGATGT+GGG 0.557016 3:-81664865 MsG0380016299.01.T01:CDS
AGCTAAGGCAAAGGAAGATG+TGG 0.560256 3:-81664866 MsG0380016299.01.T01:CDS
GATTGTGATATATTTGCGCT+TGG 0.560902 3:-81664573 MsG0380016299.01.T01:CDS
TAACCAACAAAGCCTAGTGT+TGG 0.564525 3:-81664889 MsG0380016299.01.T01:CDS
TTGAAGATATATTTAGTGAG+TGG 0.575240 3:-81664765 MsG0380016299.01.T01:CDS
TTGAATAATTGGAATGAGTG+TGG 0.579695 3:-81664639 MsG0380016299.01.T01:CDS
GATTCTAGAATCACAAAACA+TGG 0.587863 3:-81664802 MsG0380016299.01.T01:CDS
TAGTGTTGGAGCTAAGGCAA+AGG 0.590191 3:-81664875 MsG0380016299.01.T01:CDS
CTCAACACCGGAAGTAGAAG+TGG 0.592858 3:-81664718 MsG0380016299.01.T01:CDS
AGAGGAAATGTTCTCAACAC+CGG 0.624098 3:-81664730 MsG0380016299.01.T01:CDS
GAGTGGAAGTGATTGCAAAG+AGG 0.644927 3:-81664748 MsG0380016299.01.T01:CDS
TAAGGCAAAGGAAGATGTGG+GGG 0.660678 3:-81664863 MsG0380016299.01.T01:CDS
AAACGTTGAATTCATCAAGT+CGG 0.660973 3:-81665063 MsG0380016299.01.T01:CDS
CTAAGGCAAAGGAAGATGTG+GGG 0.689053 3:-81664864 MsG0380016299.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAACATGGAGTTGAAAAAA+TGG - Chr3:81664891-81664910 MsG0380016299.01.T01:CDS 25.0%
! TGGAAAAGAAAAATAAGAGT+AGG - Chr3:81665017-81665036 MsG0380016299.01.T01:CDS 25.0%
! TTGAAGATATATTTAGTGAG+TGG - Chr3:81664914-81664933 MsG0380016299.01.T01:CDS 25.0%
!! ATAAGAGTAGGTTGAATAAT+TGG - Chr3:81665029-81665048 MsG0380016299.01.T01:CDS 25.0%
AAACGTTGAATTCATCAAGT+CGG - Chr3:81664616-81664635 MsG0380016299.01.T01:CDS 30.0%
AGTGGAGATTAAAATAAGAG+TGG - Chr3:81664979-81664998 MsG0380016299.01.T01:CDS 30.0%
GATTAAAATAAGAGTGGCTA+CGG - Chr3:81664985-81665004 MsG0380016299.01.T01:CDS 30.0%
TTGAATAATTGGAATGAGTG+TGG - Chr3:81665040-81665059 MsG0380016299.01.T01:CDS 30.0%
! AAGATTTTCCAAGAATTCCT+GGG + Chr3:81664771-81664790 None:intergenic 30.0%
! TAAGATTTTCCAAGAATTCC+TGG + Chr3:81664772-81664791 None:intergenic 30.0%
! TACCAAATCAGTACCATTTT+GGG - Chr3:81664715-81664734 MsG0380016299.01.T01:CDS 30.0%
!! GATTCTAGAATCACAAAACA+TGG - Chr3:81664877-81664896 MsG0380016299.01.T01:CDS 30.0%
!!! ATACTTTTGTTGTTTCCTGT+TGG + Chr3:81664671-81664690 None:intergenic 30.0%
GATTGTGATATATTTGCGCT+TGG - Chr3:81665106-81665125 MsG0380016299.01.T01:CDS 35.0%
TTAAATGACTCGTCCCAAAA+TGG + Chr3:81664731-81664750 None:intergenic 35.0%
TTAATCTCCACTTCTACTTC+CGG + Chr3:81664971-81664990 None:intergenic 35.0%
! AGATTTTCCAAGAATTCCTG+GGG + Chr3:81664770-81664789 None:intergenic 35.0%
! CTACCAAATCAGTACCATTT+TGG - Chr3:81664714-81664733 MsG0380016299.01.T01:CDS 35.0%
!! GATTCTAGAATCACTTTCCA+TGG + Chr3:81664869-81664888 None:intergenic 35.0%
AATGAGTGTGGATGCAAGAA+TGG - Chr3:81665052-81665071 MsG0380016299.01.T01:CDS 40.0%
AGAGGAAATGTTCTCAACAC+CGG - Chr3:81664949-81664968 MsG0380016299.01.T01:CDS 40.0%
CAACTGAAGTTTGCATATGC+AGG + Chr3:81664572-81664591 None:intergenic 40.0%
CGGCAAAAAACATGTTTGCA+AGG - Chr3:81664636-81664655 MsG0380016299.01.T01:CDS 40.0%
GGGTCACAACTTATTGGAAA+AGG - Chr3:81664836-81664855 MsG0380016299.01.T01:CDS 40.0%
TAACCAACAAAGCCTAGTGT+TGG - Chr3:81664790-81664809 MsG0380016299.01.T01:CDS 40.0%
TTGGAAAAGGTGAGATTCCA+TGG - Chr3:81664849-81664868 MsG0380016299.01.T01:CDS 40.0%
! GATTTTCCAAGAATTCCTGG+GGG + Chr3:81664769-81664788 None:intergenic 40.0%
!! GTCCCAAAATGGTACTGATT+TGG + Chr3:81664720-81664739 None:intergenic 40.0%
AGCTAAGGCAAAGGAAGATG+TGG - Chr3:81664813-81664832 MsG0380016299.01.T01:CDS 45.0%
AGTGGCTACGGTTCATGAAA+TGG - Chr3:81664997-81665016 MsG0380016299.01.T01:CDS 45.0%
ATGTGGGGGTCACAACTTAT+TGG - Chr3:81664830-81664849 MsG0380016299.01.T01:CDS 45.0%
CTAAGGCAAAGGAAGATGTG+GGG - Chr3:81664815-81664834 MsG0380016299.01.T01:CDS 45.0%
GAGTGGAAGTGATTGCAAAG+AGG - Chr3:81664931-81664950 MsG0380016299.01.T01:CDS 45.0%
GCTAAGGCAAAGGAAGATGT+GGG - Chr3:81664814-81664833 MsG0380016299.01.T01:CDS 45.0%
TAAGGCAAAGGAAGATGTGG+GGG - Chr3:81664816-81664835 MsG0380016299.01.T01:CDS 45.0%
TTTGCCTTAGCTCCAACACT+AGG + Chr3:81664805-81664824 None:intergenic 45.0%
! AAAGCCTAGTGTTGGAGCTA+AGG - Chr3:81664798-81664817 MsG0380016299.01.T01:CDS 45.0%
! GCATATGCAGGTGTTTTCGA+TGG + Chr3:81664560-81664579 None:intergenic 45.0%
! TAGTGTTGGAGCTAAGGCAA+AGG - Chr3:81664804-81664823 MsG0380016299.01.T01:CDS 45.0%
CTCAACACCGGAAGTAGAAG+TGG - Chr3:81664961-81664980 MsG0380016299.01.T01:CDS 50.0%
GCAAGGCTGTATCTTCCAAC+AGG - Chr3:81664653-81664672 MsG0380016299.01.T01:CDS 50.0%
TCTAGATTGTTCTTGCCCCC+AGG - Chr3:81664751-81664770 MsG0380016299.01.T01:CDS 50.0%
TTCTTGCCCCCAGGAATTCT+TGG - Chr3:81664760-81664779 MsG0380016299.01.T01:CDS 50.0%
! GCTCCAACACTAGGCTTTGT+TGG + Chr3:81664796-81664815 None:intergenic 50.0%
ATGGCCATGATCATCGCGCT+TGG - Chr3:81665071-81665090 MsG0380016299.01.T01:CDS 55.0%
ATTTGCGCTTGGCGCAGCTT+TGG - Chr3:81665117-81665136 MsG0380016299.01.T01:CDS 55.0%
TGCACCAAGCGCGATGATCA+TGG + Chr3:81665078-81665097 None:intergenic 55.0%
! TGCGCTTGGCGCAGCTTTGG+AGG - Chr3:81665120-81665139 MsG0380016299.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 81664551 81665150 81664551 ID=MsG0380016299.01;Name=MsG0380016299.01
Chr3 mRNA 81664551 81665150 81664551 ID=MsG0380016299.01.T01;Parent=MsG0380016299.01;Name=MsG0380016299.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|199
Chr3 exon 81664551 81665150 81664551 ID=MsG0380016299.01.T01:exon:33052;Parent=MsG0380016299.01.T01
Chr3 CDS 81664551 81665150 81664551 ID=MsG0380016299.01.T01:cds;Parent=MsG0380016299.01.T01
Gene Sequence

>MsG0380016299.01.T01

ATGGATCCATCGAAAACACCTGCATATGCAAACTTCAGTTGTGAACTGAGAATAATACAAGCACGAAACGTTGAATTCATCAAGTCGGCAAAAAACATGTTTGCAAGGCTGTATCTTCCAACAGGAAACAACAAAAGTATTCAACTCAACAGCAAAAAAGTTTCTACCAAATCAGTACCATTTTGGGACGAGTCATTTAATCTAGATTGTTCTTGCCCCCAGGAATTCTTGGAAAATCTTAACCAACAAAGCCTAGTGTTGGAGCTAAGGCAAAGGAAGATGTGGGGGTCACAACTTATTGGAAAAGGTGAGATTCCATGGAAAGTGATTCTAGAATCACAAAACATGGAGTTGAAAAAATGGTTGAAGATATATTTAGTGAGTGGAAGTGATTGCAAAGAGGAAATGTTCTCAACACCGGAAGTAGAAGTGGAGATTAAAATAAGAGTGGCTACGGTTCATGAAATGGAAAAGAAAAATAAGAGTAGGTTGAATAATTGGAATGAGTGTGGATGCAAGAATGGCCATGATCATCGCGCTTGGTGCAGCGCTGAAGATTGTGATATATTTGCGCTTGGCGCAGCTTTGGAGGCTTTTTGA

Protein sequence

>MsG0380016299.01.T01

MDPSKTPAYANFSCELRIIQARNVEFIKSAKNMFARLYLPTGNNKSIQLNSKKVSTKSVPFWDESFNLDCSCPQEFLENLNQQSLVLELRQRKMWGSQLIGKGEIPWKVILESQNMELKKWLKIYLVSGSDCKEEMFSTPEVEVEIKIRVATVHEMEKKNKSRLNNWNECGCKNGHDHRAWCSAEDCDIFALGAALEAF*