Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030764.01.T01 | XP_003618488.1 | 97.17 | 318 | 5 | 1 | 1 | 314 | 1 | 318 | 0 | 636 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048802.01 | MsG0680030764.01 | 0.810120 | 1.350009e-50 | 8.070196e-48 |
| MsG0080049068.01 | MsG0680030764.01 | 0.802049 | 6.745977e-49 | 3.271937e-46 |
| MsG0080049069.01 | MsG0680030764.01 | 0.816734 | 4.746986e-52 | 3.390959e-49 |
| MsG0080049147.01 | MsG0680030764.01 | 0.809790 | 1.590010e-50 | 9.423325e-48 |
| MsG0180000114.01 | MsG0680030764.01 | 0.803928 | 2.758489e-49 | 1.403864e-46 |
| MsG0180000355.01 | MsG0680030764.01 | 0.804582 | 2.016294e-49 | 1.043462e-46 |
| MsG0180000482.01 | MsG0680030764.01 | 0.810700 | 1.011557e-50 | 6.140528e-48 |
| MsG0180001886.01 | MsG0680030764.01 | 0.803811 | 2.916864e-49 | 1.479920e-46 |
| MsG0180003741.01 | MsG0680030764.01 | 0.820979 | 5.152651e-53 | 4.139224e-50 |
| MsG0180003821.01 | MsG0680030764.01 | 0.801277 | 9.713862e-49 | 4.620301e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030764.01.T01 | MTR_6g012180 | 97.170 | 318 | 5 | 1 | 1 | 314 | 1 | 318 | 0.0 | 636 |
| MsG0680030764.01.T01 | MTR_7g010210 | 65.644 | 326 | 79 | 9 | 1 | 305 | 1 | 314 | 9.00e-140 | 398 |
| MsG0680030764.01.T01 | MTR_7g010210 | 62.058 | 311 | 61 | 6 | 1 | 305 | 1 | 260 | 2.67e-125 | 359 |
| MsG0680030764.01.T01 | MTR_0140s0030 | 53.681 | 326 | 124 | 9 | 1 | 305 | 2 | 321 | 1.08e-108 | 320 |
| MsG0680030764.01.T01 | MTR_0489s0020 | 53.681 | 326 | 124 | 9 | 1 | 305 | 2 | 321 | 1.08e-108 | 320 |
| MsG0680030764.01.T01 | MTR_3g039990 | 54.574 | 317 | 104 | 9 | 1 | 302 | 2 | 293 | 1.76e-107 | 315 |
| MsG0680030764.01.T01 | MTR_7g087130 | 57.522 | 226 | 66 | 3 | 1 | 226 | 1 | 196 | 1.23e-77 | 240 |
| MsG0680030764.01.T01 | MTR_6g012690 | 69.748 | 119 | 36 | 0 | 1 | 119 | 1 | 119 | 3.74e-59 | 193 |
| MsG0680030764.01.T01 | MTR_4g478180 | 68.908 | 119 | 37 | 0 | 1 | 119 | 2 | 120 | 3.37e-58 | 191 |
| MsG0680030764.01.T01 | MTR_1g100653 | 67.797 | 118 | 38 | 0 | 1 | 118 | 1 | 118 | 5.90e-58 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030764.01.T01 | AT3G28910 | 55.312 | 320 | 115 | 6 | 1 | 302 | 1 | 310 | 1.77e-110 | 323 |
| MsG0680030764.01.T01 | AT3G47600 | 52.960 | 321 | 121 | 6 | 1 | 298 | 1 | 314 | 3.14e-102 | 303 |
| MsG0680030764.01.T01 | AT5G62470 | 50.980 | 357 | 113 | 12 | 1 | 308 | 1 | 344 | 7.54e-99 | 295 |
| MsG0680030764.01.T01 | AT5G62470 | 50.980 | 357 | 112 | 13 | 1 | 308 | 1 | 343 | 2.22e-97 | 291 |
| MsG0680030764.01.T01 | AT1G74650 | 49.701 | 334 | 106 | 7 | 1 | 298 | 1 | 308 | 1.23e-92 | 278 |
| MsG0680030764.01.T01 | AT1G08810 | 60.829 | 217 | 35 | 4 | 1 | 217 | 1 | 167 | 2.30e-82 | 251 |
| MsG0680030764.01.T01 | AT3G47600 | 46.263 | 281 | 113 | 7 | 45 | 298 | 39 | 308 | 2.84e-67 | 213 |
| MsG0680030764.01.T01 | AT3G01140 | 49.038 | 208 | 87 | 5 | 1 | 194 | 1 | 203 | 1.79e-60 | 196 |
| MsG0680030764.01.T01 | AT5G15310 | 53.591 | 181 | 76 | 3 | 1 | 173 | 1 | 181 | 2.18e-59 | 191 |
| MsG0680030764.01.T01 | AT3G01140 | 49.038 | 208 | 87 | 5 | 1 | 194 | 44 | 246 | 2.50e-59 | 195 |
Find 42 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTAAAGAAGGATCAAA+TGG | 0.460507 | 6:+8751478 | MsG0680030764.01.T01:CDS |
| AAAGCTGTTCCTACCAATAC+TGG | 0.417394 | 6:+8750015 | MsG0680030764.01.T01:CDS |
| AACATAGCTCGTTTGTTGAA+AGG | 0.493776 | 6:+8751442 | MsG0680030764.01.T01:CDS |
| AACCAATAGCTAGAGGACAA+TGG | 0.597136 | 6:+8751248 | MsG0680030764.01.T01:CDS |
| AAGAACATGGTCCTGGAAAT+TGG | 0.229465 | 6:+8749992 | MsG0680030764.01.T01:CDS |
| AAGTAATTACCAGTATTGGT+AGG | 0.620581 | 6:-8750024 | None:intergenic |
| ACACCTTCTTTATCACAACA+TGG | 0.621958 | 6:-8749916 | None:intergenic |
| ACCAAAATGATATCTTCTTC+AGG | 0.368108 | 6:-8749958 | None:intergenic |
| ACCAATAGCTAGAGGACAAT+GGG | 0.439491 | 6:+8751249 | MsG0680030764.01.T01:CDS |
| ACTTCTTCTGAATCATGTTT+AGG | 0.323676 | 6:+8751202 | MsG0680030764.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCTAAAGAAGGATCAAA+TGG | + | Chr6:8751478-8751497 | MsG0680030764.01.T01:CDS | 30.0% | |
| AAATTACTTTGACATGTGCT+AGG | - | Chr6:8750927-8750946 | None:intergenic | 30.0% | |
| ACCAAAATGATATCTTCTTC+AGG | - | Chr6:8749961-8749980 | None:intergenic | 30.0% | |
| ACTTCTTCTGAATCATGTTT+AGG | + | Chr6:8751202-8751221 | MsG0680030764.01.T01:CDS | 30.0% | |
| AGAGTAGTAGAATCAGAATT+TGG | - | Chr6:8751631-8751650 | None:intergenic | 30.0% | |
| CTTTCTCAATGCTTGAAAAA+TGG | + | Chr6:8751695-8751714 | MsG0680030764.01.T01:CDS | 30.0% | |
| GAGAGTAAACCTGAAAATAT+TGG | + | Chr6:8751661-8751680 | MsG0680030764.01.T01:CDS | 30.0% | |
| TAAAAAAAACTAGGTGGTTG+TGG | - | Chr6:8750711-8750730 | None:intergenic | 30.0% | |
| TCGTATTGTTAACGCAATTT+AGG | - | Chr6:8750836-8750855 | None:intergenic | 30.0% | |
| TTACCATTTGATCCTTCTTT+AGG | - | Chr6:8751484-8751503 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 8749904 | 8751780 | 8749904 | ID=MsG0680030764.01;Name=MsG0680030764.01 |
| Chr6 | mRNA | 8749904 | 8751780 | 8749904 | ID=MsG0680030764.01.T01;Parent=MsG0680030764.01;Name=MsG0680030764.01.T01;_AED=0.31;_eAED=0.37;_QI=0|0|0|1|1|1|3|0|314 |
| Chr6 | exon | 8749904 | 8750036 | 8749904 | ID=MsG0680030764.01.T01:exon:7214;Parent=MsG0680030764.01.T01 |
| Chr6 | exon | 8750542 | 8750671 | 8750542 | ID=MsG0680030764.01.T01:exon:7215;Parent=MsG0680030764.01.T01 |
| Chr6 | exon | 8751099 | 8751780 | 8751099 | ID=MsG0680030764.01.T01:exon:7216;Parent=MsG0680030764.01.T01 |
| Chr6 | CDS | 8749904 | 8750036 | 8749904 | ID=MsG0680030764.01.T01:cds;Parent=MsG0680030764.01.T01 |
| Chr6 | CDS | 8750542 | 8750671 | 8750542 | ID=MsG0680030764.01.T01:cds;Parent=MsG0680030764.01.T01 |
| Chr6 | CDS | 8751099 | 8751780 | 8751099 | ID=MsG0680030764.01.T01:cds;Parent=MsG0680030764.01.T01 |
| Gene Sequence |
>MsG0680030764.01.T01 ATGGTTAGACCACCATGTTGTGATAAAGAAGGTGTGAAAAAAGGACCTTGGACTCCTGAAGAAGATATCATTTTGGTTTCTTATATTCAAGAACATGGTCCTGGAAATTGGAAAGCTGTTCCTACCAATACTGGTTTGTTAAGGTGTAGTAAGAGTTGCAGACTTAGATGGACAAATTATCTAAGGCCAGGAATCAAAAGGGGTAACTTCACTGACCAAGAAGAGAAAATGATTATCCATCTTCAAGCTCTTTTAGGAAACAGATGGGCTGCCATAGCTGCATACCTTCCACAAAGAACAGACAATGACATCAAAAACTATTGGAACACTTATTTGAAGAAAAAGCTGAAAAAATTGGAAACAAGTACTTCTTCTGAATCATGTTTAGGTCATGATGAATTCTCAGTTTCTCAACCAATAGCTAGAGGACAATGGGAAAGAAGGCTTCAAACTGATATTCACATGGCCAAAAAAGCACTTAGTGAAGCACTTCAACCAGAAAAAAGCACTTCTAGTAGCAACTTAATGATACCACTTGAATCAAATTTTTCTAGTGAAAGCTCTTTTTGTTCAACAAAACCTATGTCTTATGCATCAAGTGCTGATAACATAGCTCGTTTGTTGAAAGGTTGGATGAAAAATAAACCTAAAGAAGGATCAAATGGTAATAACACATATGTGACACCAAATTATGAGACTAGTGCTTCTAGTGAAGGAATGGAAAAAGGGTCAACAAGTGTTGAATTATCTGAGACTTTTGAATCTTTGTTTGGTTATGAGTCTTTTGATTCACCAAATTCTGATTCTACTACTCTTTTTCAAGATGAGAGTAAACCTGAAAATATTGGTGAAATTATGCCTTTCTCAATGCTTGAAAAATGGCTTCTAGATGATGGTGCTTGTCAAGAGAAAGTTGGTCTTAGTGAAATTAATTACATATTTTGA |
| Protein sequence |
>MsG0680030764.01.T01 MVRPPCCDKEGVKKGPWTPEEDIILVSYIQEHGPGNWKAVPTNTGLLRCSKSCRLRWTNYLRPGIKRGNFTDQEEKMIIHLQALLGNRWAAIAAYLPQRTDNDIKNYWNTYLKKKLKKLETSTSSESCLGHDEFSVSQPIARGQWERRLQTDIHMAKKALSEALQPEKSTSSSNLMIPLESNFSSESSFCSTKPMSYASSADNIARLLKGWMKNKPKEGSNGNNTYVTPNYETSASSEGMEKGSTSVELSETFESLFGYESFDSPNSDSTTLFQDESKPENIGEIMPFSMLEKWLLDDGACQEKVGLSEINYIF* |
