AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049147.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTAATTATAACCTATTT+AGG - contig94end:1007-1026 None:intergenic 15.0%
!! TGAAAATGATATTAAAAAGT+TGG + contig94end:1200-1219 MsG0080049147.01.T01:intron 15.0%
!!! AAAAAGTATTAATTTGGAAT+AGG + contig94end:226-245 MsG0080049147.01.T01:CDS 15.0%
!!! AATACAATCTTCATTTTTTA+AGG - contig94end:1163-1182 None:intergenic 15.0%
!!! AATTAATACTTTTTTCAAAC+TGG - contig94end:220-239 None:intergenic 15.0%
!!! ATTAATACTTTTTTCAAACT+GGG - contig94end:219-238 None:intergenic 15.0%
!!! GTTTGAAAAAAGTATTAATT+TGG + contig94end:220-239 MsG0080049147.01.T01:CDS 15.0%
!!! AAAAATGAGCTTTAATCAAA+TGG - contig94end:188-207 None:intergenic 20.0%
!!! AAAATGAGCTTTAATCAAAT+GGG - contig94end:187-206 None:intergenic 20.0%
! GATATTAAAAAGTTGGAGAT+GGG + contig94end:1207-1226 MsG0080049147.01.T01:intron 25.0%
! TGATATTAAAAAGTTGGAGA+TGG + contig94end:1206-1225 MsG0080049147.01.T01:intron 25.0%
! TTATGCTTAATAGTTCCATA+AGG + contig94end:762-781 MsG0080049147.01.T01:intron 25.0%
! TTATTAGAGGTGATAAAGTT+GGG + contig94end:607-626 MsG0080049147.01.T01:intron 25.0%
! TTCTAAGTATTTATCTAGAG+AGG + contig94end:119-138 MsG0080049147.01.T01:CDS 25.0%
!! AAATGAGCTTTAATCAAATG+GGG - contig94end:186-205 None:intergenic 25.0%
!! TTTTTGTGCATCATCTAATT+TGG + contig94end:836-855 MsG0080049147.01.T01:intron 25.0%
AAACACAAGAGGTTCTATAA+AGG - contig94end:89-108 None:intergenic 30.0%
AAACCAACTTGAAAACCTTA+TGG - contig94end:780-799 None:intergenic 30.0%
AACACAAGAGGTTCTATAAA+GGG - contig94end:88-107 None:intergenic 30.0%
CTTATTAGAGGTGATAAAGT+TGG + contig94end:606-625 MsG0080049147.01.T01:intron 30.0%
GAAAAGTTAGGCAAAAAGAT+TGG + contig94end:1241-1260 MsG0080049147.01.T01:intron 30.0%
GTTAGTAAAGAGCTTATGTT+TGG + contig94end:1459-1478 MsG0080049147.01.T01:intron 30.0%
TATAATTGTGAGATGAGTCT+GGG + contig94end:1056-1075 MsG0080049147.01.T01:intron 30.0%
TTATAATTGTGAGATGAGTC+TGG + contig94end:1055-1074 MsG0080049147.01.T01:intron 30.0%
TTGTTTATGAGACATACACA+AGG + contig94end:702-721 MsG0080049147.01.T01:intron 30.0%
! GAAAGTTTTTGTTGACAATG+AGG + contig94end:498-517 MsG0080049147.01.T01:CDS 30.0%
! GTTCTTTTCAATTCAACAAC+TGG + contig94end:27-46 MsG0080049147.01.T01:CDS 30.0%
! TAATTTGGACTAGAAGAGTT+TGG + contig94end:851-870 MsG0080049147.01.T01:intron 30.0%
! TAGCTTGGAAACATTCTTTT+GGG + contig94end:1264-1283 MsG0080049147.01.T01:CDS 30.0%
!! GTATGGTTTTCTGAAAAGTT+AGG + contig94end:1229-1248 MsG0080049147.01.T01:intron 30.0%
!! TTAAAGCTCATTTTTCAGCT+AGG + contig94end:193-212 MsG0080049147.01.T01:CDS 30.0%
!!! ATTACTGTTTTCAAGTCTGT+TGG + contig94end:648-667 MsG0080049147.01.T01:intron 30.0%
!!! GAGATAATGGGATTGATTTT+TGG - contig94end:1648-1667 None:intergenic 30.0%
AGTTGAAAAGGCGAAAACTT+TGG + contig94end:251-270 MsG0080049147.01.T01:CDS 35.0%
ATAATGCAATGCGCCTAAAT+AGG + contig94end:991-1010 MsG0080049147.01.T01:intron 35.0%
ATAATTGTGAGATGAGTCTG+GGG + contig94end:1057-1076 MsG0080049147.01.T01:intron 35.0%
ATGAGGAGTTACTTGTACTA+GGG + contig94end:806-825 MsG0080049147.01.T01:intron 35.0%
GGATAGTGAGATTCTTGTTA+TGG + contig94end:140-159 MsG0080049147.01.T01:CDS 35.0%
GTAAAGAGCTTATGTTTGGA+TGG + contig94end:1463-1482 MsG0080049147.01.T01:intron 35.0%
GTGATCAAGGAAGATGAATT+GGG + contig94end:568-587 MsG0080049147.01.T01:intron 35.0%
TAAAAAGTTGGAGATGGGTA+TGG + contig94end:1212-1231 MsG0080049147.01.T01:intron 35.0%
TAGGGTATGCTAGAGATAAT+GGG - contig94end:1660-1679 None:intergenic 35.0%
TATAGAACCTCTTGTGTTTC+TGG + contig94end:90-109 MsG0080049147.01.T01:CDS 35.0%
TCTATAAAGGGTAAGTTCAG+TGG - contig94end:76-95 None:intergenic 35.0%
TGATCAAGGAAGATGAATTG+GGG + contig94end:569-588 MsG0080049147.01.T01:intron 35.0%
TGTGATCAAGGAAGATGAAT+TGG + contig94end:567-586 MsG0080049147.01.T01:intron 35.0%
TTAGGGTATGCTAGAGATAA+TGG - contig94end:1661-1680 None:intergenic 35.0%
TTGGAATAGGACAGTTGAAA+AGG + contig94end:239-258 MsG0080049147.01.T01:CDS 35.0%
! AGTGAGATTCTTGTTATGGT+TGG + contig94end:144-163 MsG0080049147.01.T01:CDS 35.0%
! GATGGATTTGTGCCAAAATT+TGG - contig94end:1805-1824 None:intergenic 35.0%
! GTAGCTTGGAAACATTCTTT+TGG + contig94end:1263-1282 MsG0080049147.01.T01:CDS 35.0%
! GTCTTTTAAGCATTCGATGA+AGG + contig94end:447-466 MsG0080049147.01.T01:intron 35.0%
!! AACTTGTTGGAGCTTATTAG+AGG + contig94end:594-613 MsG0080049147.01.T01:intron 35.0%
!!! GTTCCATAAGGTTTTCAAGT+TGG + contig94end:774-793 MsG0080049147.01.T01:intron 35.0%
AAGAGCTTATGTTTGGATGG+AGG + contig94end:1466-1485 MsG0080049147.01.T01:intron 40.0%
ACACAACCTCCACATAACTT+AGG - contig94end:1679-1698 None:intergenic 40.0%
AGAGCTTATGTTTGGATGGA+GGG + contig94end:1467-1486 MsG0080049147.01.T01:intron 40.0%
AGTCAGAGACTTGAAACAGT+TGG + contig94end:1367-1386 MsG0080049147.01.T01:CDS 40.0%
AGTGGAATCCATAGAAGCTA+GGG - contig94end:58-77 None:intergenic 40.0%
ATCAAGGAAGATGAATTGGG+GGG + contig94end:571-590 MsG0080049147.01.T01:intron 40.0%
CACAACCTCCACATAACTTA+GGG - contig94end:1678-1697 None:intergenic 40.0%
CTAGCATACCCTAAGTTATG+TGG + contig94end:1667-1686 MsG0080049147.01.T01:intron 40.0%
GATCAAGGAAGATGAATTGG+GGG + contig94end:570-589 MsG0080049147.01.T01:intron 40.0%
GATGAGGAGTTACTTGTACT+AGG + contig94end:805-824 MsG0080049147.01.T01:intron 40.0%
GGTTTCAGTACTTGCAAAAG+AGG + contig94end:1079-1098 MsG0080049147.01.T01:intron 40.0%
TAATTGTGAGATGAGTCTGG+GGG + contig94end:1058-1077 MsG0080049147.01.T01:intron 40.0%
TGAGACATACACAAGGACAT+AGG + contig94end:709-728 MsG0080049147.01.T01:intron 40.0%
TGGATGAGGTTGTTGGATTT+GGG + contig94end:340-359 MsG0080049147.01.T01:intron 40.0%
TGTGAATGAGCAGGATAACT+GGG + contig94end:1696-1715 MsG0080049147.01.T01:intron 40.0%
TTCTGCTGTTGTTGATATGC+TGG + contig94end:671-690 MsG0080049147.01.T01:intron 40.0%
TTGGATGAGGTTGTTGGATT+TGG + contig94end:339-358 MsG0080049147.01.T01:intron 40.0%
! ACTTGAAACAGTTGGTTCAG+GGG + contig94end:1375-1394 MsG0080049147.01.T01:CDS 40.0%
! AGACTTGAAACAGTTGGTTC+AGG + contig94end:1373-1392 MsG0080049147.01.T01:CDS 40.0%
! AGAGGCTTTTAAACTCCTAG+TGG + contig94end:1756-1775 MsG0080049147.01.T01:CDS 40.0%
! AGCGATGAATTTTCGCTTTC+AGG + contig94end:291-310 MsG0080049147.01.T01:CDS 40.0%
! AGGCAAAAAGATTGGTAGCT+TGG + contig94end:1249-1268 MsG0080049147.01.T01:intron 40.0%
! ATTCTTGTTATGGTTGGTGC+TGG + contig94end:150-169 MsG0080049147.01.T01:CDS 40.0%
! CTTGGAAACATTCTTTTGGG+AGG + contig94end:1267-1286 MsG0080049147.01.T01:CDS 40.0%
! GACTTGAAACAGTTGGTTCA+GGG + contig94end:1374-1393 MsG0080049147.01.T01:CDS 40.0%
! GAGGCTTTTAAACTCCTAGT+GGG + contig94end:1757-1776 MsG0080049147.01.T01:CDS 40.0%
! GCGATGAATTTTCGCTTTCA+GGG + contig94end:292-311 MsG0080049147.01.T01:CDS 40.0%
! TGTGAGCTGTGCTACTAATT+CGG + contig94end:367-386 MsG0080049147.01.T01:intron 40.0%
! TTCTTGTTATGGTTGGTGCT+GGG + contig94end:151-170 MsG0080049147.01.T01:CDS 40.0%
!! AACCTCTTGTGTTTCTGGTT+TGG + contig94end:95-114 MsG0080049147.01.T01:CDS 40.0%
!! ATGATGAGGCTTTGAAAAGG+GGG + contig94end:476-495 MsG0080049147.01.T01:CDS 40.0%
!! CAAGTTGGTTTGATGAGATG+AGG + contig94end:789-808 MsG0080049147.01.T01:intron 40.0%
!! CGATGATGAGGCTTTGAAAA+GGG + contig94end:474-493 MsG0080049147.01.T01:CDS 40.0%
!! CTTTCAGGGTTGAGTTTTGA+GGG + contig94end:306-325 MsG0080049147.01.T01:CDS 40.0%
!! GATGATGAGGCTTTGAAAAG+GGG + contig94end:475-494 MsG0080049147.01.T01:CDS 40.0%
!! TTTTGAAAGGGGTGTGATCA+AGG + contig94end:555-574 MsG0080049147.01.T01:intron 40.0%
!! TTTTGAGGGTTGTGGGAATT+TGG + contig94end:320-339 MsG0080049147.01.T01:CDS 40.0%
ACATAGCTACCTTGAACACC+TGG - contig94end:9-28 None:intergenic 45.0%
AGCCAAACCAGAAACACAAG+AGG - contig94end:100-119 None:intergenic 45.0%
AGCTGAGTATTCACTGACAG+AGG + contig94end:1738-1757 MsG0080049147.01.T01:CDS 45.0%
AGGGGTGGATGACATTGAAT+TGG + contig94end:1393-1412 MsG0080049147.01.T01:CDS 45.0%
AGTTGGGAGAAGCAGTTTAG+AGG + contig94end:623-642 MsG0080049147.01.T01:intron 45.0%
ATGAATTGGGGGGAACTTGT+TGG + contig94end:581-600 MsG0080049147.01.T01:intron 45.0%
CAGTGGAATCCATAGAAGCT+AGG - contig94end:59-78 None:intergenic 45.0%
CATTGAATTGGCAGTGCAGA+AGG + contig94end:1405-1424 MsG0080049147.01.T01:intron 45.0%
GAGCTTATGTTTGGATGGAG+GGG + contig94end:1468-1487 MsG0080049147.01.T01:intron 45.0%
GATGAAGGAATGCGATGATG+AGG + contig94end:462-481 MsG0080049147.01.T01:CDS 45.0%
GCAGAAGGAGTTTATTCCGT+GGG + contig94end:1420-1439 MsG0080049147.01.T01:intron 45.0%
GCATACCCTAAGTTATGTGG+AGG + contig94end:1670-1689 MsG0080049147.01.T01:intron 45.0%
GGGAATTTGGATGAGGTTGT+TGG + contig94end:333-352 MsG0080049147.01.T01:intron 45.0%
GGTTGGAGCTCATTTGGATT+TGG + contig94end:421-440 MsG0080049147.01.T01:intron 45.0%
GTGTGAATGAGCAGGATAAC+TGG + contig94end:1695-1714 MsG0080049147.01.T01:intron 45.0%
GTTGAAGGTTGGAGCTCATT+TGG + contig94end:415-434 MsG0080049147.01.T01:intron 45.0%
TCATGCAATATGTCCTCCCA+CGG - contig94end:1439-1458 None:intergenic 45.0%
TGCAGAAGGAGTTTATTCCG+TGG + contig94end:1419-1438 MsG0080049147.01.T01:intron 45.0%
TGGTCAAACCCTAGCTTCTA+TGG + contig94end:47-66 MsG0080049147.01.T01:CDS 45.0%
TGTTGACAATGAGGCTGCAT+TGG + contig94end:507-526 MsG0080049147.01.T01:CDS 45.0%
TTATGTTTGGATGGAGGGGA+GGG + contig94end:1472-1491 MsG0080049147.01.T01:intron 45.0%
! AGCTTGTGGGTGCTTTTGAA+AGG + contig94end:542-561 MsG0080049147.01.T01:CDS 45.0%
! CGAGAGGAGCTTCCAAATTT+TGG + contig94end:1790-1809 MsG0080049147.01.T01:CDS 45.0%
! GCTTTCAGGGTTGAGTTTTG+AGG + contig94end:305-324 MsG0080049147.01.T01:CDS 45.0%
! GGAGCTCATTTGGATTTGGT+TGG + contig94end:425-444 MsG0080049147.01.T01:intron 45.0%
!! CTTGTGGGTGCTTTTGAAAG+GGG + contig94end:544-563 MsG0080049147.01.T01:CDS 45.0%
!! GCGATGATGAGGCTTTGAAA+AGG + contig94end:473-492 MsG0080049147.01.T01:CDS 45.0%
!! GCTTGTGGGTGCTTTTGAAA+GGG + contig94end:543-562 MsG0080049147.01.T01:CDS 45.0%
!! TCTTGTTATGGTTGGTGCTG+GGG + contig94end:152-171 MsG0080049147.01.T01:CDS 45.0%
!!! GGTTGAGTTTTGAGGGTTGT+GGG + contig94end:313-332 MsG0080049147.01.T01:CDS 45.0%
AAGGGTGAGAGGTTGAAGGT+TGG + contig94end:404-423 MsG0080049147.01.T01:intron 50.0%
CAGGATAACTGGGTATGTGG+AGG + contig94end:1706-1725 MsG0080049147.01.T01:intron 50.0%
CTTATGTTTGGATGGAGGGG+AGG + contig94end:1471-1490 MsG0080049147.01.T01:intron 50.0%
GAAGGAGTTTATTCCGTGGG+AGG + contig94end:1423-1442 MsG0080049147.01.T01:intron 50.0%
GAGCAGGATAACTGGGTATG+TGG + contig94end:1703-1722 MsG0080049147.01.T01:intron 50.0%
GGGTTGTGGGAATTTGGATG+AGG + contig94end:326-345 MsG0080049147.01.T01:CDS 50.0%
TGGAGGTTGTGTGAATGAGC+AGG + contig94end:1687-1706 MsG0080049147.01.T01:intron 50.0%
TGTGAAGGGTGAGAGGTTGA+AGG + contig94end:400-419 MsG0080049147.01.T01:intron 50.0%
! TGAAACAGTTGGTTCAGGGG+TGG + contig94end:1378-1397 MsG0080049147.01.T01:CDS 50.0%
!! AGGCTGCATTGGTTGAAGCA+GGG + contig94end:518-537 MsG0080049147.01.T01:CDS 50.0%
!! TTTTGGGAGGAACCTTCGAG+CGG + contig94end:1280-1299 MsG0080049147.01.T01:CDS 50.0%
!!! GGGTTGAGTTTTGAGGGTTG+TGG + contig94end:312-331 MsG0080049147.01.T01:CDS 50.0%
CTCGTTGTTGCACTCCCACT+AGG - contig94end:1774-1793 None:intergenic 55.0%
GTTGAAGCAGGGGAGCTTGT+GGG + contig94end:529-548 MsG0080049147.01.T01:CDS 55.0%
TCGGAGACAGCGCTTGTGAA+GGG + contig94end:386-405 MsG0080049147.01.T01:intron 55.0%
TTCGGAGACAGCGCTTGTGA+AGG + contig94end:385-404 MsG0080049147.01.T01:intron 55.0%
! AGTGGGAGTGCAACAACGAG+AGG + contig94end:1774-1793 MsG0080049147.01.T01:CDS 55.0%
!! GAGGCTGCATTGGTTGAAGC+AGG + contig94end:517-536 MsG0080049147.01.T01:CDS 55.0%
!! GGCTGCATTGGTTGAAGCAG+GGG + contig94end:519-538 MsG0080049147.01.T01:CDS 55.0%
CAGCGCTTGTGAAGGGTGAG+AGG + contig94end:393-412 MsG0080049147.01.T01:intron 60.0%
GGTTGAAGCAGGGGAGCTTG+TGG + contig94end:528-547 MsG0080049147.01.T01:CDS 60.0%
!! TATGGTTGGTGCTGGGGCTC+TGG + contig94end:158-177 MsG0080049147.01.T01:CDS 60.0%
AACCTTCGAGCGGAGGGGTG+GGG + contig94end:1290-1309 MsG0080049147.01.T01:CDS 65.0%
GAACCTTCGAGCGGAGGGGT+GGG + contig94end:1289-1308 MsG0080049147.01.T01:CDS 65.0%
GGAGGAACCTTCGAGCGGAG+GGG + contig94end:1285-1304 MsG0080049147.01.T01:CDS 65.0%
GGGAGGAACCTTCGAGCGGA+GGG + contig94end:1284-1303 MsG0080049147.01.T01:CDS 65.0%
TGGGAGGAACCTTCGAGCGG+AGG + contig94end:1283-1302 MsG0080049147.01.T01:CDS 65.0%
!! GGGTGGGGGAGGGGTATTCA+AGG + contig94end:1311-1330 MsG0080049147.01.T01:CDS 65.0%
ACCTTCGAGCGGAGGGGTGG+GGG + contig94end:1291-1310 MsG0080049147.01.T01:CDS 70.0%
GGAACCTTCGAGCGGAGGGG+TGG + contig94end:1288-1307 MsG0080049147.01.T01:CDS 70.0%
ACCCCCACCCCTCCGCTCGA+AGG - contig94end:1295-1314 None:intergenic 75.0%
TCGAGCGGAGGGGTGGGGGT+GGG + contig94end:1295-1314 MsG0080049147.01.T01:CDS 75.0%
TTCGAGCGGAGGGGTGGGGG+TGG + contig94end:1294-1313 MsG0080049147.01.T01:CDS 75.0%
! CGAGCGGAGGGGTGGGGGTG+GGG + contig94end:1296-1315 MsG0080049147.01.T01:CDS 80.0%
! GAGCGGAGGGGTGGGGGTGG+GGG + contig94end:1297-1316 MsG0080049147.01.T01:CDS 80.0%
! GAGGGGTGGGGGTGGGGGAG+GGG + contig94end:1302-1321 MsG0080049147.01.T01:CDS 80.0%
! GGAGGGGTGGGGGTGGGGGA+GGG + contig94end:1301-1320 MsG0080049147.01.T01:CDS 80.0%
! CGGAGGGGTGGGGGTGGGGG+AGG + contig94end:1300-1319 MsG0080049147.01.T01:CDS 85.0%
Chromosome Type Strat End Strand Name
contig94end gene 1 1831 1 ID=MsG0080049147.01;
contig94end mRNA 1 1831 1 ID=MsG0080049147.01.T01;Parent=MsG0080049147.01
contig94end CDS 1 347 1 ID=MsG0080049147.01.T01.cds;Parent=MsG0080049147.01.T01
contig94end CDS 457 565 457 ID=MsG0080049147.01.T01.cds;Parent=MsG0080049147.01.T01
contig94end CDS 1251 1417 1251 ID=MsG0080049147.01.T01.cds;Parent=MsG0080049147.01.T01
contig94end CDS 1709 1831 1709 ID=MsG0080049147.01.T01.cds;Parent=MsG0080049147.01.T01
Gene Sequence

>MsG0080049147.01.T01

ATTTACCAGGTGTTCAAGGTAGCTATGTTCTTTTCAATTCAACAACTGGTCAAACCCTAGCTTCTATGGATTCCACTGAACTTACCCTTTATAGAACCTCTTGTGTTTCTGGTTTGGCTTCTAAGTATTTATCTAGAGAGGATAGTGAGATTCTTGTTATGGTTGGTGCTGGGGCTCTGGCACCCCATTTGATTAAAGCTCATTTTTCAGCTAGGCCCAGTTTGAAAAAAGTATTAATTTGGAATAGGACAGTTGAAAAGGCGAAAACTTTGGCGAATAAATTGAGTGAAAGCGATGAATTTTCGCTTTCAGGGTTGAGTTTTGAGGGTTGTGGGAATTTGGATGAGCATTCGATGAAGGAATGCGATGATGAGGCTTTGAAAAGGGGGAAAGTTTTTGTTGACAATGAGGCTGCATTGGTTGAAGCAGGGGAGCTTGTGGGTGCTTTTGAAAGGGGCAAAAAGATTGGTAGCTTGGAAACATTCTTTTGGGAGGAACCTTCGAGCGGAGGGGTGGGGGTGGGGGAGGGGTATTCAAGGAGCAATTCAATCAGTCTATTCAAGAGACACGAAAGTCAGAGACTTGAAACAGTTGGTTCAGGGGTGGATGACATTGAATTGGCAGATAACTGGGTATGTGGAGGAGATAAATCAGCTGAGTATTCACTGACAGAGGCTTTTAAACTCCTAGTGGGAGTGCAACAACGAGAGGAGCTTCCAAATTTTGGCACAAATCCATCCTGTTGA

Protein sequence

>MsG0080049147.01.T01

IYQVFKVAMFFSIQQLVKP*LLWIPLNLPFIEPLVFLVWLLSIYLERIVRFLLWLVLGLWHPI*LKLIFQLGPV*KKY*FGIGQLKRRKLWRIN*VKAMNFRFQG*VLRVVGIWMSIR*RNAMMRL*KGGKFLLTMRLHWLKQGSLWVLLKGAKRLVAWKHSFGRNLRAEGWGWGRGIQGAIQSVYSRDTKVRDLKQLVQGWMTLNWQITGYVEEINQLSIH*QRLLNS*WECNNERSFQILAQIHPV