Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031544.01.T01 | XP_039691116.1 | 82.166 | 157 | 28 | 0 | 1 | 157 | 485 | 641 | 1.14E-80 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031544.01.T01 | Q9SG80 | 52.532 | 158 | 72 | 2 | 1 | 157 | 500 | 655 | 6.70E-48 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031544.01.T01 | A0A072U6X3 | 82.166 | 157 | 28 | 0 | 1 | 157 | 508 | 664 | 1.19e-80 | 260 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011175.01 | MsG0680031544.01 | 0.807429 | 5.077471e-50 | 2.827411e-47 |
| MsG0680031544.01 | MsG0680032771.01 | 0.803321 | 3.685583e-49 | 1.846481e-46 |
| MsG0680031544.01 | MsG0780038276.01 | 0.800209 | 1.604269e-48 | 7.426587e-46 |
| MsG0680031544.01 | MsG0780039522.01 | 0.810213 | 1.289284e-50 | 7.725271e-48 |
| MsG0680031544.01 | MsG0780041507.01 | 0.802968 | 4.361231e-49 | 2.165324e-46 |
| MsG0680031544.01 | MsG0880042700.01 | 0.806511 | 7.937627e-50 | 4.316404e-47 |
| MsG0480020025.01 | MsG0680031544.01 | 0.803632 | 3.178238e-49 | 1.605088e-46 |
| MsG0480023660.01 | MsG0680031544.01 | 0.803098 | 4.098897e-49 | 2.041793e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031544.01.T01 | MTR_6g025730 | 82.166 | 157 | 28 | 0 | 1 | 157 | 508 | 664 | 3.01e-84 | 260 |
| MsG0680031544.01.T01 | MTR_6g025790 | 80.892 | 157 | 30 | 0 | 1 | 157 | 509 | 665 | 1.54e-81 | 253 |
| MsG0680031544.01.T01 | MTR_7g087880 | 57.233 | 159 | 68 | 0 | 1 | 159 | 185 | 343 | 5.92e-55 | 176 |
| MsG0680031544.01.T01 | MTR_7g088410 | 55.346 | 159 | 69 | 2 | 1 | 157 | 494 | 652 | 2.03e-52 | 176 |
| MsG0680031544.01.T01 | MTR_0176s0070 | 56.604 | 159 | 69 | 0 | 1 | 159 | 497 | 655 | 1.78e-50 | 171 |
| MsG0680031544.01.T01 | MTR_7g088440 | 57.595 | 158 | 64 | 3 | 1 | 157 | 492 | 647 | 7.11e-50 | 169 |
| MsG0680031544.01.T01 | MTR_7g088420 | 55.063 | 158 | 69 | 2 | 1 | 157 | 492 | 648 | 1.42e-48 | 166 |
| MsG0680031544.01.T01 | MTR_7g087960 | 55.063 | 158 | 71 | 0 | 2 | 159 | 486 | 643 | 1.74e-48 | 166 |
| MsG0680031544.01.T01 | MTR_7g088470 | 52.597 | 154 | 71 | 2 | 1 | 153 | 493 | 645 | 4.64e-45 | 157 |
| MsG0680031544.01.T01 | MTR_7g087890 | 59.259 | 135 | 55 | 0 | 1 | 135 | 506 | 640 | 3.87e-42 | 149 |
| MsG0680031544.01.T01 | MTR_2g090585 | 41.176 | 170 | 62 | 2 | 1 | 159 | 481 | 623 | 1.81e-30 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031544.01.T01 | AT3G10740 | 52.532 | 158 | 72 | 2 | 1 | 157 | 500 | 655 | 6.83e-49 | 167 |
| MsG0680031544.01.T01 | AT3G10740 | 52.532 | 158 | 72 | 2 | 1 | 157 | 500 | 655 | 6.83e-49 | 167 |
| MsG0680031544.01.T01 | AT3G10740 | 52.532 | 158 | 72 | 2 | 1 | 157 | 519 | 674 | 7.80e-49 | 167 |
| MsG0680031544.01.T01 | AT3G10740 | 52.532 | 158 | 72 | 2 | 1 | 157 | 519 | 674 | 7.80e-49 | 167 |
| MsG0680031544.01.T01 | AT5G26120 | 48.734 | 158 | 76 | 3 | 1 | 157 | 499 | 652 | 1.78e-42 | 149 |
Find 33 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATATTAGATACCTTGTT+TGG | 0.317437 | 6:-22020255 | None:intergenic |
| TTGCTGCATCTGCAATTTCA+TGG | 0.317494 | 6:+22019749 | MsG0680031544.01.T01:CDS |
| CATTGATATGGCTATACAAT+AGG | 0.329539 | 6:+22020428 | MsG0680031544.01.T01:CDS |
| TTCTTAACTGAGCTCTCTTT+TGG | 0.335778 | 6:-22020357 | None:intergenic |
| TCATGGAAATCTTCTGTAGA+TGG | 0.339834 | 6:+22019766 | MsG0680031544.01.T01:CDS |
| GACTTTGCAGGTTGCAAACT+TGG | 0.370386 | 6:+22020110 | MsG0680031544.01.T01:intron |
| CTCATTCACAGTCATTGATA+TGG | 0.374471 | 6:+22020416 | MsG0680031544.01.T01:CDS |
| TTTGATTCTCACCAAGTGTA+TGG | 0.392598 | 6:+22019637 | MsG0680031544.01.T01:CDS |
| GGATGATGTAAGCACTGTTT+TGG | 0.426324 | 6:-22020202 | None:intergenic |
| TGGGATTTCTATTGAAGGGT+TGG | 0.439796 | 6:+22020152 | MsG0680031544.01.T01:CDS |
| TCAATGACTGTGAATGAGTA+TGG | 0.442532 | 6:-22020411 | None:intergenic |
| ATTGATATGGCTATACAATA+GGG | 0.449834 | 6:+22020429 | MsG0680031544.01.T01:CDS |
| AGAGTTCTCATCCATTGGAT+TGG | 0.456710 | 6:-22020223 | None:intergenic |
| GAATCAAATACAATTGCATC+TGG | 0.461409 | 6:-22019622 | None:intergenic |
| AGCTTAATCAACCAATGGCT+TGG | 0.466395 | 6:-22019664 | None:intergenic |
| TCTTTGTGAATACCAATGAT+AGG | 0.471767 | 6:+22019493 | MsG0680031544.01.T01:CDS |
| GAGAGCTCAGTTAAGAACTC+TGG | 0.476221 | 6:+22020363 | MsG0680031544.01.T01:CDS |
| AATTACATACCTCCTATCAT+TGG | 0.494366 | 6:-22019505 | None:intergenic |
| AGTATTGGGATTTCTATTGA+AGG | 0.506060 | 6:+22020147 | MsG0680031544.01.T01:CDS |
| ATCAAGTGTGGTTCTTTCAA+AGG | 0.507203 | 6:+22020176 | MsG0680031544.01.T01:CDS |
| GGCTTGGAGTTCCATACACT+TGG | 0.521056 | 6:-22019648 | None:intergenic |
| ATCATTGGTATTCACAAAGA+GGG | 0.528718 | 6:-22019490 | None:intergenic |
| TGAAAAGCTTAATCAACCAA+TGG | 0.551071 | 6:-22019669 | None:intergenic |
| TATCATTGGTATTCACAAAG+AGG | 0.564809 | 6:-22019491 | None:intergenic |
| GTATTGGGATTTCTATTGAA+GGG | 0.565048 | 6:+22020148 | MsG0680031544.01.T01:CDS |
| CTCAGTTAAGAACTCTGGAA+AGG | 0.581587 | 6:+22020368 | MsG0680031544.01.T01:CDS |
| ATTGCAGATGCAGCAAGTGT+AGG | 0.584793 | 6:-22019742 | None:intergenic |
| GCTTACATCATCCAATCCAA+TGG | 0.587368 | 6:+22020212 | MsG0680031544.01.T01:CDS |
| CTCTGGAAAGGAAATGAGTG+TGG | 0.596355 | 6:+22020380 | MsG0680031544.01.T01:CDS |
| TGTATGGAACTCCAAGCCAT+TGG | 0.601011 | 6:+22019653 | MsG0680031544.01.T01:CDS |
| TGAAGGGTTGGAATCAAGTG+TGG | 0.623408 | 6:+22020164 | MsG0680031544.01.T01:CDS |
| TTGTGAATACCAATGATAGG+AGG | 0.637170 | 6:+22019496 | MsG0680031544.01.T01:CDS |
| TCATTGGTATTCACAAAGAG+GGG | 0.674141 | 6:-22019489 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTAACCTTAAATATTTA+AGG | + | Chr6:22019839-22019858 | MsG0680031544.01.T01:intron | 10.0% |
| !! | AATCATTTGTGACATATTTA+AGG | + | Chr6:22020025-22020044 | MsG0680031544.01.T01:intron | 20.0% |
| !! | TGATTGTCCTTAAATATTTA+AGG | - | Chr6:22019849-22019868 | None:intergenic | 20.0% |
| !!! | TAAATCAGTTTTAAGGATTA+AGG | + | Chr6:22019789-22019808 | MsG0680031544.01.T01:CDS | 20.0% |
| !!! | TTTGATTTTCCATTGTAATT+AGG | + | Chr6:22019593-22019612 | MsG0680031544.01.T01:intron | 20.0% |
| ! | ATAAGTATTTCCTCTTATTG+TGG | + | Chr6:22020054-22020073 | MsG0680031544.01.T01:intron | 25.0% |
| ! | ATTTCTCATTAGTTACAAAC+TGG | + | Chr6:22020086-22020105 | MsG0680031544.01.T01:intron | 25.0% |
| ! | GATAGATTCTAATATACTCA+AGG | - | Chr6:22019879-22019898 | None:intergenic | 25.0% |
| ! | TATACAACTGAATAAAAGTG+TGG | - | Chr6:22020298-22020317 | None:intergenic | 25.0% |
| !! | AAGAGTTGACTTTAAAAATG+TGG | - | Chr6:22019710-22019729 | None:intergenic | 25.0% |
| !! | TAGATGGTAAATCAGTTTTA+AGG | + | Chr6:22019782-22019801 | MsG0680031544.01.T01:CDS | 25.0% |
| !! | TGATGCTTGATTATCTATTT+TGG | - | Chr6:22019573-22019592 | None:intergenic | 25.0% |
| !!! | TTTTAAGGATTAAGGTAAAG+TGG | + | Chr6:22019797-22019816 | MsG0680031544.01.T01:intron | 25.0% |
| AATTACATACCTCCTATCAT+TGG | - | Chr6:22019508-22019527 | None:intergenic | 30.0% | |
| ACAAAAAAGCAGTGAGTATT+GGG | + | Chr6:22020133-22020152 | MsG0680031544.01.T01:CDS | 30.0% | |
| ACAAACATAAGATGCATATG+AGG | - | Chr6:22019940-22019959 | None:intergenic | 30.0% | |
| ATCATTGGTATTCACAAAGA+GGG | - | Chr6:22019493-22019512 | None:intergenic | 30.0% | |
| GAATCAAATACAATTGCATC+TGG | - | Chr6:22019625-22019644 | None:intergenic | 30.0% | |
| GTATTGGGATTTCTATTGAA+GGG | + | Chr6:22020148-22020167 | MsG0680031544.01.T01:CDS | 30.0% | |
| GTTGTATATTCTGACACTTA+AGG | + | Chr6:22020310-22020329 | MsG0680031544.01.T01:intron | 30.0% | |
| TACAATTACACCACAATAAG+AGG | - | Chr6:22020067-22020086 | None:intergenic | 30.0% | |
| TATCATTGGTATTCACAAAG+AGG | - | Chr6:22019494-22019513 | None:intergenic | 30.0% | |
| TCTTTGTGAATACCAATGAT+AGG | + | Chr6:22019493-22019512 | MsG0680031544.01.T01:CDS | 30.0% | |
| TGAAAAGCTTAATCAACCAA+TGG | - | Chr6:22019672-22019691 | None:intergenic | 30.0% | |
| TGCATATTAGATACCTTGTT+TGG | - | Chr6:22020258-22020277 | None:intergenic | 30.0% | |
| ! | AGTATTGGGATTTCTATTGA+AGG | + | Chr6:22020147-22020166 | MsG0680031544.01.T01:CDS | 30.0% |
| ! | GATTTTCCATTGTAATTAGG+TGG | + | Chr6:22019596-22019615 | MsG0680031544.01.T01:intron | 30.0% |
| ! | TCATCGACTGTTAATCAATT+TGG | + | Chr6:22019968-22019987 | MsG0680031544.01.T01:intron | 30.0% |
| ! | TTGGTTGATTAAGCTTTTCA+AGG | + | Chr6:22019672-22019691 | MsG0680031544.01.T01:CDS | 30.0% |
| ATCAAGTGTGGTTCTTTCAA+AGG | + | Chr6:22020176-22020195 | MsG0680031544.01.T01:CDS | 35.0% | |
| CTCATTCACAGTCATTGATA+TGG | + | Chr6:22020416-22020435 | MsG0680031544.01.T01:CDS | 35.0% | |
| GAAAAAGAGTTCTCATCCAT+TGG | - | Chr6:22020231-22020250 | None:intergenic | 35.0% | |
| GACAAAAAAGCAGTGAGTAT+TGG | + | Chr6:22020132-22020151 | MsG0680031544.01.T01:CDS | 35.0% | |
| GAGGACTGTTCTTCTAATTT+GGG | - | Chr6:22019921-22019940 | None:intergenic | 35.0% | |
| TCAATGACTGTGAATGAGTA+TGG | - | Chr6:22020414-22020433 | None:intergenic | 35.0% | |
| TCATGGAAATCTTCTGTAGA+TGG | + | Chr6:22019766-22019785 | MsG0680031544.01.T01:CDS | 35.0% | |
| TCATTGGTATTCACAAAGAG+GGG | - | Chr6:22019492-22019511 | None:intergenic | 35.0% | |
| TGAGGACTGTTCTTCTAATT+TGG | - | Chr6:22019922-22019941 | None:intergenic | 35.0% | |
| TTGTGAATACCAATGATAGG+AGG | + | Chr6:22019496-22019515 | MsG0680031544.01.T01:CDS | 35.0% | |
| TTTGATTCTCACCAAGTGTA+TGG | + | Chr6:22019637-22019656 | MsG0680031544.01.T01:CDS | 35.0% | |
| ! | TTCTTAACTGAGCTCTCTTT+TGG | - | Chr6:22020360-22020379 | None:intergenic | 35.0% |
| !! | AAGCTTTTCAAGGAATCAAG+TGG | + | Chr6:22019682-22019701 | MsG0680031544.01.T01:CDS | 35.0% |
| AGAGTTCTCATCCATTGGAT+TGG | - | Chr6:22020226-22020245 | None:intergenic | 40.0% | |
| AGCTTAATCAACCAATGGCT+TGG | - | Chr6:22019667-22019686 | None:intergenic | 40.0% | |
| GCTTACATCATCCAATCCAA+TGG | + | Chr6:22020212-22020231 | MsG0680031544.01.T01:CDS | 40.0% | |
| TGGAGTCCACCTAATTACAA+TGG | - | Chr6:22019605-22019624 | None:intergenic | 40.0% | |
| TGGGATTTCTATTGAAGGGT+TGG | + | Chr6:22020152-22020171 | MsG0680031544.01.T01:CDS | 40.0% | |
| TTACAAACTGGTGACTTTGC+AGG | + | Chr6:22020098-22020117 | MsG0680031544.01.T01:intron | 40.0% | |
| TTGCTGCATCTGCAATTTCA+TGG | + | Chr6:22019749-22019768 | MsG0680031544.01.T01:CDS | 40.0% | |
| ! | CTCAGTTAAGAACTCTGGAA+AGG | + | Chr6:22020368-22020387 | MsG0680031544.01.T01:CDS | 40.0% |
| !! | GGATGATGTAAGCACTGTTT+TGG | - | Chr6:22020205-22020224 | None:intergenic | 40.0% |
| !!! | CTCTTTTTCAGAGCCAAACA+AGG | + | Chr6:22020242-22020261 | MsG0680031544.01.T01:CDS | 40.0% |
| ATTGCAGATGCAGCAAGTGT+AGG | - | Chr6:22019745-22019764 | None:intergenic | 45.0% | |
| GACTTTGCAGGTTGCAAACT+TGG | + | Chr6:22020110-22020129 | MsG0680031544.01.T01:intron | 45.0% | |
| GAGAGCTCAGTTAAGAACTC+TGG | + | Chr6:22020363-22020382 | MsG0680031544.01.T01:CDS | 45.0% | |
| TGAAGGGTTGGAATCAAGTG+TGG | + | Chr6:22020164-22020183 | MsG0680031544.01.T01:CDS | 45.0% | |
| TGTATGGAACTCCAAGCCAT+TGG | + | Chr6:22019653-22019672 | MsG0680031544.01.T01:CDS | 45.0% | |
| ! | CTCTGGAAAGGAAATGAGTG+TGG | + | Chr6:22020380-22020399 | MsG0680031544.01.T01:CDS | 45.0% |
| GGCTTGGAGTTCCATACACT+TGG | - | Chr6:22019651-22019670 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 22019474 | 22020449 | 22019474 | ID=MsG0680031544.01;Name=MsG0680031544.01 |
| Chr6 | mRNA | 22019474 | 22020449 | 22019474 | ID=MsG0680031544.01.T01;Parent=MsG0680031544.01;Name=MsG0680031544.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|0.75|4|0|160 |
| Chr6 | exon | 22019474 | 22019517 | 22019474 | ID=MsG0680031544.01.T01:exon:13844;Parent=MsG0680031544.01.T01 |
| Chr6 | exon | 22019615 | 22019810 | 22019615 | ID=MsG0680031544.01.T01:exon:13845;Parent=MsG0680031544.01.T01 |
| Chr6 | exon | 22020120 | 22020263 | 22020120 | ID=MsG0680031544.01.T01:exon:13846;Parent=MsG0680031544.01.T01 |
| Chr6 | exon | 22020351 | 22020449 | 22020351 | ID=MsG0680031544.01.T01:exon:13847;Parent=MsG0680031544.01.T01 |
| Chr6 | CDS | 22019474 | 22019517 | 22019474 | ID=MsG0680031544.01.T01:cds;Parent=MsG0680031544.01.T01 |
| Chr6 | CDS | 22019615 | 22019810 | 22019615 | ID=MsG0680031544.01.T01:cds;Parent=MsG0680031544.01.T01 |
| Chr6 | CDS | 22020120 | 22020263 | 22020120 | ID=MsG0680031544.01.T01:cds;Parent=MsG0680031544.01.T01 |
| Chr6 | CDS | 22020351 | 22020449 | 22020351 | ID=MsG0680031544.01.T01:cds;Parent=MsG0680031544.01.T01 |
| Gene Sequence |
| Protein sequence |
