AlfalfaGEDB | MsG0680032771.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032771.01

KEGG: K00472
GO: GO:0005506 GO:0016491 GO:0016705 GO:0031418 GO:0055114
PFAM: PF13640

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032771.01.T01	XP_013452366.1	69.133	392	24	6	1	390	1	297	1.93E-177	505

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032771.01.T01	Q8GXT7	42.231	251	121	4	145	390	60	291	4.80E-62	204

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032771.01.T01	A0A072U9B5	69.133	392	24	6	1	390	1	297	9.21e-178	505

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0680032771.01	0.806426	8.272528e-50	4.488761e-47
MsG0080047957.01	MsG0680032771.01	0.813840	2.088332e-51	1.378665e-48
MsG0080047959.01	MsG0680032771.01	0.804266	2.346490e-49	1.204481e-46
MsG0080048152.01	MsG0680032771.01	0.802652	5.067376e-49	2.495777e-46
MsG0080048462.01	MsG0680032771.01	0.812370	4.387337e-51	2.784447e-48
MsG0080048683.01	MsG0680032771.01	0.800984	1.114954e-48	5.264346e-46
MsG0080048774.01	MsG0680032771.01	0.809433	1.897213e-50	1.113761e-47
MsG0080048826.01	MsG0680032771.01	0.804972	1.670844e-49	8.734823e-47
MsG0080048856.01	MsG0680032771.01	0.837162	6.116929e-57	7.878387e-54
MsG0180000139.01	MsG0680032771.01	0.808973	2.380117e-50	1.380148e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032771.01.T01	MTR_6g053720	69.133	392	24	6	1	390	1	297	0.0	505
MsG0680032771.01.T01	MTR_4g118560	40.304	263	134	6	147	390	40	298	5.26e-56	186
MsG0680032771.01.T01	MTR_6g009440	37.405	262	142	7	147	390	54	311	2.68e-51	174
MsG0680032771.01.T01	MTR_2g020800	38.403	263	139	6	147	390	43	301	4.45e-51	173
MsG0680032771.01.T01	MTR_2g020800	36.981	265	142	6	147	390	43	303	1.52e-50	172
MsG0680032771.01.T01	MTR_3g114670	36.735	147	86	3	191	332	130	274	2.08e-25	104
MsG0680032771.01.T01	MTR_8g074890	34.722	144	91	2	192	332	128	271	1.44e-23	99.4
MsG0680032771.01.T01	MTR_5g022960	34.752	141	89	2	193	330	141	281	1.45e-23	99.8
MsG0680032771.01.T01	MTR_8g074810	34.722	144	91	2	191	331	128	271	2.97e-23	98.6
MsG0680032771.01.T01	MTR_8g074880	34.483	145	92	2	191	332	176	320	1.96e-22	97.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032771.01.T01	AT4G25600	42.231	251	121	4	145	390	60	291	4.90e-63	204
MsG0680032771.01.T01	AT4G25600	48.485	198	91	2	193	390	44	230	4.22e-62	199
MsG0680032771.01.T01	AT4G25600	48.485	198	91	2	193	390	53	239	4.31e-62	200
MsG0680032771.01.T01	AT4G25600	48.485	198	91	2	193	390	41	227	4.37e-62	199
MsG0680032771.01.T01	AT3G28490	38.931	262	134	6	147	390	35	288	2.78e-56	186
MsG0680032771.01.T01	AT3G28480	37.023	262	141	6	147	390	59	314	1.98e-53	180
MsG0680032771.01.T01	AT3G28480	42.000	200	110	3	193	390	127	322	3.56e-53	179
MsG0680032771.01.T01	AT3G06300	44.390	205	105	4	191	390	99	299	4.24e-53	179
MsG0680032771.01.T01	AT5G18900	40.488	205	113	4	191	390	98	298	6.34e-49	167
MsG0680032771.01.T01	AT4G35810	37.415	147	89	2	191	334	141	287	3.48e-25	104

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Find 93 sgRNAs with CRISPR-Local

Find 412 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAAATTGGGATCAAC+CGG	0.420089	6:-49407534	MsG0680032771.01.T01:CDS
AAAGAAAGAGGAAACTTCAT+TGG	0.368859	6:-49409207	MsG0680032771.01.T01:CDS
AAATTGTAGAAATAACAGTG+AGG	0.789611	6:-49407033	MsG0680032771.01.T01:CDS
AATCCTCTTTCCTGAATCAG+TGG	0.600382	6:-49407457	MsG0680032771.01.T01:CDS
AATGCATGCTGATGAGATGT+TGG	0.593376	6:-49406711	MsG0680032771.01.T01:exon
ACATATCGCCTTTAAGGACA+GGG	0.680203	6:+49406922	None:intergenic
ACCTTGGTTGCCAAGAGATT+TGG	0.242434	6:+49411758	None:intergenic
ACGGTCTGCAATTGCAATTG+TGG	0.632041	6:-49408057	MsG0680032771.01.T01:CDS
ACTATGTTCCTCAATTAACT+AGG	0.593251	6:-49406586	MsG0680032771.01.T01:three_prime_UTR
AGAAAGGTGTTAGAAGCGAG+AGG	0.551794	6:+49411969	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTAAATCTACGCCAAA+TGG	+	Chr6:49407231-49407250	None:intergenic	30.0%
AAAGAAAGAGGAAACTTCAT+TGG	-	Chr6:49409356-49409375	MsG0680032771.01.T01:intron	30.0%
AACAAGTAATTGCAACATGT+TGG	+	Chr6:49410679-49410698	None:intergenic	30.0%
AATGTTTATCCAGAATCAGT+TGG	+	Chr6:49408198-49408217	None:intergenic	30.0%
AATTAGAGTGAAATTAGGGA+AGG	+	Chr6:49406648-49406667	None:intergenic	30.0%
ACAACGTTTCACAAAGTAAA+CGG	+	Chr6:49409711-49409730	None:intergenic	30.0%
ACATTTACTTGCAAAACACT+TGG	+	Chr6:49410230-49410249	None:intergenic	30.0%
ACTATGTTCCTCAATTAACT+AGG	-	Chr6:49411977-49411996	MsG0680032771.01.T01:CDS	30.0%
ACTTCAAGAAAACTTACTGT+AGG	-	Chr6:49409569-49409588	MsG0680032771.01.T01:intron	30.0%
AGAATCAACTAGCTGTAAAA+AGG	+	Chr6:49408676-49408695	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	49406424	49412161	49406424	ID=MsG0680032771.01;Name=MsG0680032771.01
Chr6	mRNA	49406424	49412161	49406424	ID=MsG0680032771.01.T01;Parent=MsG0680032771.01;Name=MsG0680032771.01.T01;_AED=0.40;_eAED=0.47;_QI=132\|0.25\|0.33\|1\|0.5\|0.55\|9\|298\|390
Chr6	exon	49411962	49412161	49411962	ID=MsG0680032771.01.T01:exon:13913;Parent=MsG0680032771.01.T01
Chr6	exon	49411755	49411870	49411755	ID=MsG0680032771.01.T01:exon:13914;Parent=MsG0680032771.01.T01
Chr6	exon	49409759	49409835	49409759	ID=MsG0680032771.01.T01:exon:13915;Parent=MsG0680032771.01.T01
Chr6	exon	49409199	49409363	49409199	ID=MsG0680032771.01.T01:exon:13916;Parent=MsG0680032771.01.T01
Chr6	exon	49408461	49408506	49408461	ID=MsG0680032771.01.T01:exon:13917;Parent=MsG0680032771.01.T01
Chr6	exon	49408052	49408155	49408052	ID=MsG0680032771.01.T01:exon:13918;Parent=MsG0680032771.01.T01
Chr6	exon	49407724	49407781	49407724	ID=MsG0680032771.01.T01:exon:13919;Parent=MsG0680032771.01.T01
Chr6	exon	49407446	49407630	49407446	ID=MsG0680032771.01.T01:exon:13920;Parent=MsG0680032771.01.T01
Chr6	exon	49406424	49407075	49406424	ID=MsG0680032771.01.T01:exon:13921;Parent=MsG0680032771.01.T01
Chr6	five_prime_UTR	49412030	49412161	49412030	ID=MsG0680032771.01.T01:five_prime_utr;Parent=MsG0680032771.01.T01
Chr6	CDS	49411962	49412029	49411962	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49411755	49411870	49411755	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49409759	49409835	49409759	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49409199	49409363	49409199	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49408461	49408506	49408461	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49408052	49408155	49408052	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49407724	49407781	49407724	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49407446	49407630	49407446	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	CDS	49406722	49407075	49406722	ID=MsG0680032771.01.T01:cds;Parent=MsG0680032771.01.T01
Chr6	three_prime_UTR	49406424	49406721	49406424	ID=MsG0680032771.01.T01:three_prime_utr;Parent=MsG0680032771.01.T01

Gene Sequence

>MsG0680032771.01.T01

ATGTCTTTACTTACCTTTATCCTCATCACCTTCTTCACCCTCTCGCTTCTAACACCTTTCTCCGACAGGAAAGAATTAAGGAACAAAAATGAAAATGTGTTAAAGCAGTTAAGAAATTCTGTTTATTATTCAAACAGAATTGATCCTTCACGTGTTGTCCAAATCTCTTGGCAACCAAGGTCAGGGTCTTTTTGTACAAAGGCTTTTTGTCGGATAAGGAGTGTGACTACCTTATTTATTTGGTATGTTGCAGTCTCATTGATTCTCAAACAAATAAGTCACTTTTGTAGTTATGTTTTTGTTTTTCATTTGTTTTCAACTTGTTTTGTCGTTGATTTTTACTCTATTGTTTTTGCCAAGGCACAAGAAAAATCATCTGGAAATGGTGGCTATTCAAAGAAAGAGGAAACTTCATTGGATATGGATGTTGACTTGGTTCTTAATTGGATACCAGAGCCTTTTCTACCAAGCGGTCCTAACCTGCTCTCGGCTCTGCTGTTTGTGACTCAGCAGAAAGATATTGAAAAACGGTCTGCAATCGCAATTGTACGGTCTGCAATTGCAATTGTGGCTGCGGATGATATTGTCAAGAGGATTGAAGAAAGACTTTCGGTTTGGACTTTTCTTCCTAAAGAGAACAGCAAGCCTTTACAGGTCATGCATTATGGGCTTGAAAAAGATAGGCGGAACTTAGATTATTTCACAAACAAAACCAAATTGGGATCAACCGGACCTTTGATGGCAACAGTGATTCTACATCTCTCGAATGCTACTCAAGGGGGTCAAATCCTCTTTCCTGAATCAGTGGTGAGTACGGCAACCAAGAGTAGCAGCTGGTCAAATTGTAGAAATAACAGTGAGGTTCTCCAACCAGTTAAAGGGAATGCAGTTCTGTTTTTCTCTCTTAACCTAAATACATCTCCTGACAAGAGTAGCTTACACGCTCGATGCCCTGTCCTTAAAGGCGATATGTGGTCTGCAATTAAATTCTTCTATACAAGATCGATTAGTGGAGGGAAAGTCTCAACTACGCCGGAAGATGAAGATTGTACTGATGAGGATGATAGTTGTCCCGCTTGGGCAGCAACGGGAGAATGTCAACGGAACCCTGTCTACATGGTTGGATCTCCTGATTACTATGGTACATGTCGGAAAAGCTGCAATGCATGCTGA

Protein sequence

>MsG0680032771.01.T01

MSLLTFILITFFTLSLLTPFSDRKELRNKNENVLKQLRNSVYYSNRIDPSRVVQISWQPRSGSFCTKAFCRIRSVTTLFIWYVAVSLILKQISHFCSYVFVFHLFSTCFVVDFYSIVFAKAQEKSSGNGGYSKKEETSLDMDVDLVLNWIPEPFLPSGPNLLSALLFVTQQKDIEKRSAIAIVRSAIAIVAADDIVKRIEERLSVWTFLPKENSKPLQVMHYGLEKDRRNLDYFTNKTKLGSTGPLMATVILHLSNATQGGQILFPESVVSTATKSSSWSNCRNNSEVLQPVKGNAVLFFSLNLNTSPDKSSLHARCPVLKGDMWSAIKFFYTRSISGGKVSTTPEDEDCTDEDDSCPAWAATGECQRNPVYMVGSPDYYGTCRKSCNAC*