AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039522.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039522.01.T01 MTR_7g075140 94.286 70 3 1 1 69 814 883 1.57e-36 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTGTTCTTTCAAATC+AGG 0.300884 7:-65234539 None:intergenic
TCAAAAGCAGGGTGATTCTA+TGG 0.360002 7:+65234718 MsG0780039522.01.T01:CDS
TATGGATGAGGATGAGGAAG+AGG 0.407916 7:+65234736 MsG0780039522.01.T01:CDS
TGATTCTATGGATGAGGATG+AGG 0.507637 7:+65234730 MsG0780039522.01.T01:CDS
AATATCCGATGAAGAAACTC+AGG 0.517234 7:+65234673 MsG0780039522.01.T01:CDS
TGTCGAGGAGGATCAAAAGC+AGG 0.527285 7:+65234706 MsG0780039522.01.T01:CDS
CTGAATCAGATGAACCACTA+TGG 0.535870 7:-65234589 None:intergenic
GAAGATGCTGCTTGTCGAGA+GGG 0.537000 7:+65234560 MsG0780039522.01.T01:CDS
GCAGGGTGATTCTATGGATG+AGG 0.560143 7:+65234724 MsG0780039522.01.T01:CDS
CGAGCACTAAGTGAGATGAT+TGG 0.582146 7:+65234506 None:intergenic
TGAAGATGCTGCTTGTCGAG+AGG 0.618982 7:+65234559 MsG0780039522.01.T01:CDS
AGTGACACTGGCGATGATGA+TGG 0.623013 7:+65234626 MsG0780039522.01.T01:CDS
GACACCCTGAGTTTCTTCAT+CGG 0.628971 7:-65234678 None:intergenic
ACTGAGAGTTGTAGTGACAC+TGG 0.633200 7:+65234614 MsG0780039522.01.T01:CDS
GTCGAGGAGGATCAAAAGCA+GGG 0.635450 7:+65234707 MsG0780039522.01.T01:CDS
CGAGAGGGAGAAGACCATAG+TGG 0.640161 7:+65234575 MsG0780039522.01.T01:CDS
TCAGGGTGTCGACCATGTCG+AGG 0.655020 7:+65234691 MsG0780039522.01.T01:CDS
GGGTGTCGACCATGTCGAGG+AGG 0.674979 7:+65234694 MsG0780039522.01.T01:CDS
ATATCCGATGAAGAAACTCA+GGG 0.704443 7:+65234674 MsG0780039522.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAATTGTTCTTTCAAATC+AGG - Chr7:65234542-65234561 None:intergenic 25.0%
AATATCCGATGAAGAAACTC+AGG + Chr7:65234673-65234692 MsG0780039522.01.T01:CDS 35.0%
ATATCCGATGAAGAAACTCA+GGG + Chr7:65234674-65234693 MsG0780039522.01.T01:CDS 35.0%
CTGAATCAGATGAACCACTA+TGG - Chr7:65234592-65234611 None:intergenic 40.0%
TCAAAAGCAGGGTGATTCTA+TGG + Chr7:65234718-65234737 MsG0780039522.01.T01:CDS 40.0%
TGATTCTATGGATGAGGATG+AGG + Chr7:65234730-65234749 MsG0780039522.01.T01:CDS 40.0%
ACTGAGAGTTGTAGTGACAC+TGG + Chr7:65234614-65234633 MsG0780039522.01.T01:CDS 45.0%
GACACCCTGAGTTTCTTCAT+CGG - Chr7:65234681-65234700 None:intergenic 45.0%
TATGGATGAGGATGAGGAAG+AGG + Chr7:65234736-65234755 MsG0780039522.01.T01:CDS 45.0%
AGTGACACTGGCGATGATGA+TGG + Chr7:65234626-65234645 MsG0780039522.01.T01:CDS 50.0%
GCAGGGTGATTCTATGGATG+AGG + Chr7:65234724-65234743 MsG0780039522.01.T01:CDS 50.0%
GTCGAGGAGGATCAAAAGCA+GGG + Chr7:65234707-65234726 MsG0780039522.01.T01:CDS 50.0%
TGTCGAGGAGGATCAAAAGC+AGG + Chr7:65234706-65234725 MsG0780039522.01.T01:CDS 50.0%
! GAAGATGCTGCTTGTCGAGA+GGG + Chr7:65234560-65234579 MsG0780039522.01.T01:CDS 50.0%
! GCTTTTGATCCTCCTCGACA+TGG - Chr7:65234706-65234725 None:intergenic 50.0%
! TGAAGATGCTGCTTGTCGAG+AGG + Chr7:65234559-65234578 MsG0780039522.01.T01:CDS 50.0%
CGAGAGGGAGAAGACCATAG+TGG + Chr7:65234575-65234594 MsG0780039522.01.T01:CDS 55.0%
!! TCAGGGTGTCGACCATGTCG+AGG + Chr7:65234691-65234710 MsG0780039522.01.T01:CDS 60.0%
!! GGGTGTCGACCATGTCGAGG+AGG + Chr7:65234694-65234713 MsG0780039522.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 65234521 65234778 65234521 ID=MsG0780039522.01;Name=MsG0780039522.01
Chr7 mRNA 65234521 65234778 65234521 ID=MsG0780039522.01.T01;Parent=MsG0780039522.01;Name=MsG0780039522.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|85
Chr7 exon 65234521 65234778 65234521 ID=MsG0780039522.01.T01:exon:33755;Parent=MsG0780039522.01.T01
Chr7 CDS 65234521 65234778 65234521 ID=MsG0780039522.01.T01:cds;Parent=MsG0780039522.01.T01
Gene Sequence

>MsG0780039522.01.T01

ATGATTGGCATTGTTGCTCCTGATTTGAAAGAACAATTTGAAGATGCTGCTTGTCGAGAGGGAGAAGACCATAGTGGTTCATCTGATTCAGATACTGAGAGTTGTAGTGACACTGGCGATGATGATGGTAGAGTGATCACAAAGCGTGAATCAATATCCGATGAAGAAACTCAGGGTGTCGACCATGTCGAGGAGGATCAAAAGCAGGGTGATTCTATGGATGAGGATGAGGAAGAGGAAGACACTCAAGAAGATTGA

Protein sequence

>MsG0780039522.01.T01

MIGIVAPDLKEQFEDAACREGEDHSGSSDSDTESCSDTGDDDGRVITKRESISDEETQGVDHVEEDQKQGDSMDEDEEEEDTQED*