AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020025.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020025.01.T01 MTR_0001s0430 92.647 136 10 0 22 157 694 829 2.49e-85 266
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020025.01.T01 AT1G30610 35.714 112 63 3 22 128 863 970 1.54e-15 73.2
MsG0480020025.01.T01 AT1G30610 35.714 112 63 3 22 128 891 998 1.70e-15 73.2

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATGACAACTTTGAAAA+TGG 0.176434 4:-32190333 None:intergenic
ATTATTGGAAATTACATTAC+TGG 0.261573 4:-32190421 None:intergenic
TGGATTTGGCACACTATTAT+TGG 0.269795 4:-32190436 None:intergenic
AATAGATGTCAAACTTTATC+AGG 0.300445 4:+32190106 None:intergenic
GAAAATGGTTGCAAATCTTT+TGG 0.337695 4:-32190318 None:intergenic
AAACAAACAAGTGCTGGATT+TGG 0.355004 4:-32190450 None:intergenic
CTCTCCTTGATTAGAGAAAC+TGG 0.363503 4:-32190231 None:intergenic
TGAATTAAACAAACAAGTGC+TGG 0.426635 4:-32190456 None:intergenic
TCCACCAGTTTCTCTAATCA+AGG 0.432976 4:+32190227 MsG0480020025.01.T01:CDS
GAGATCATTTCATTGACAAA+TGG 0.442455 4:+32190135 MsG0480020025.01.T01:CDS
TACAAATGTTCACCTAGTAT+TGG 0.449620 4:-32190575 None:intergenic
TTCAGAAGGATACAGTTGTC+AGG 0.462834 4:+32190385 MsG0480020025.01.T01:CDS
TTTGCTGAATCCATGTCAGC+TGG 0.488530 4:-32190516 None:intergenic
ATTTGCATTAGAAAGCAAAC+AGG 0.507249 4:+32190545 MsG0480020025.01.T01:CDS
TGCTTCCATGTTATCTCCAA+TGG 0.513064 4:-32190183 None:intergenic
TGATCTCAGTCCAGCTGACA+TGG 0.513159 4:+32190506 MsG0480020025.01.T01:CDS
ACAGGAAGTAAACCAATACT+AGG 0.533197 4:+32190563 MsG0480020025.01.T01:CDS
GTGTGCTAATTGTGATGCAT+TGG 0.537013 4:-32190296 None:intergenic
CTCTTCCTGAAAACAAAGCA+AGG 0.558653 4:-32190160 None:intergenic
AAATTCTCAACGGTTTCAGA+AGG 0.569611 4:+32190371 MsG0480020025.01.T01:CDS
GGAGATAACATGGAAGCACT+TGG 0.590719 4:+32190188 MsG0480020025.01.T01:CDS
AAGAGCCATTGGAGATAACA+TGG 0.594911 4:+32190178 MsG0480020025.01.T01:CDS
TATTCAAAGAAAATTCTCAA+CGG 0.622614 4:+32190361 MsG0480020025.01.T01:CDS
CAGTTTCTCTAATCAAGGAG+AGG 0.624084 4:+32190232 MsG0480020025.01.T01:CDS
GTTCTGTACAAAGCTCGAGA+AGG 0.644676 4:+32190254 MsG0480020025.01.T01:CDS
TGGTGGAATTCGATCTGTAG+CGG 0.649596 4:-32190211 None:intergenic
GTCAGCTGGACTGAGATCAG+TGG 0.685003 4:-32190502 None:intergenic
TCCTTGATTAGAGAAACTGG+TGG 0.721292 4:-32190228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTGGAAATTACATTAC+TGG - Chr4:32190424-32190443 None:intergenic 20.0%
!! TATTCAAAGAAAATTCTCAA+CGG + Chr4:32190361-32190380 MsG0480020025.01.T01:CDS 20.0%
ATTTGCATTAGAAAGCAAAC+AGG + Chr4:32190545-32190564 MsG0480020025.01.T01:CDS 30.0%
GAGATCATTTCATTGACAAA+TGG + Chr4:32190135-32190154 MsG0480020025.01.T01:CDS 30.0%
TGAATTAAACAAACAAGTGC+TGG - Chr4:32190459-32190478 None:intergenic 30.0%
! AACCATGACAACTTTGAAAA+TGG - Chr4:32190336-32190355 None:intergenic 30.0%
! AACCATTTTCAAAGTTGTCA+TGG + Chr4:32190331-32190350 MsG0480020025.01.T01:CDS 30.0%
! GAAAATGGTTGCAAATCTTT+TGG - Chr4:32190321-32190340 None:intergenic 30.0%
AAATTCTCAACGGTTTCAGA+AGG + Chr4:32190371-32190390 MsG0480020025.01.T01:CDS 35.0%
TGGATTTGGCACACTATTAT+TGG - Chr4:32190439-32190458 None:intergenic 35.0%
!! AAACAAACAAGTGCTGGATT+TGG - Chr4:32190453-32190472 None:intergenic 35.0%
AAGAGCCATTGGAGATAACA+TGG + Chr4:32190178-32190197 MsG0480020025.01.T01:CDS 40.0%
CAGTTTCTCTAATCAAGGAG+AGG + Chr4:32190232-32190251 MsG0480020025.01.T01:CDS 40.0%
CTCTCCTTGATTAGAGAAAC+TGG - Chr4:32190234-32190253 None:intergenic 40.0%
CTCTTCCTGAAAACAAAGCA+AGG - Chr4:32190163-32190182 None:intergenic 40.0%
GTGTGCTAATTGTGATGCAT+TGG - Chr4:32190299-32190318 None:intergenic 40.0%
TCCACCAGTTTCTCTAATCA+AGG + Chr4:32190227-32190246 MsG0480020025.01.T01:CDS 40.0%
TCCTTGATTAGAGAAACTGG+TGG - Chr4:32190231-32190250 None:intergenic 40.0%
TGCTTCCATGTTATCTCCAA+TGG - Chr4:32190186-32190205 None:intergenic 40.0%
TTCAGAAGGATACAGTTGTC+AGG + Chr4:32190385-32190404 MsG0480020025.01.T01:CDS 40.0%
!! TTGTTTTCAGGAAGAGCCAT+TGG + Chr4:32190167-32190186 MsG0480020025.01.T01:CDS 40.0%
!!! TGGAACCTTGCTTTGTTTTC+AGG + Chr4:32190155-32190174 MsG0480020025.01.T01:CDS 40.0%
GTTCTGTACAAAGCTCGAGA+AGG + Chr4:32190254-32190273 MsG0480020025.01.T01:CDS 45.0%
TGGTGGAATTCGATCTGTAG+CGG - Chr4:32190214-32190233 None:intergenic 45.0%
TTTGCTGAATCCATGTCAGC+TGG - Chr4:32190519-32190538 None:intergenic 45.0%
!! GGAGATAACATGGAAGCACT+TGG + Chr4:32190188-32190207 MsG0480020025.01.T01:CDS 45.0%
TGATCTCAGTCCAGCTGACA+TGG + Chr4:32190506-32190525 MsG0480020025.01.T01:CDS 50.0%
GTCAGCTGGACTGAGATCAG+TGG - Chr4:32190505-32190524 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 32190111 32190584 32190111 ID=MsG0480020025.01;Name=MsG0480020025.01
Chr4 mRNA 32190111 32190584 32190111 ID=MsG0480020025.01.T01;Parent=MsG0480020025.01;Name=MsG0480020025.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|157
Chr4 exon 32190111 32190584 32190111 ID=MsG0480020025.01.T01:exon:10443;Parent=MsG0480020025.01.T01
Chr4 CDS 32190111 32190584 32190111 ID=MsG0480020025.01.T01:cds;Parent=MsG0480020025.01.T01
Gene Sequence

>MsG0480020025.01.T01

ATGTCAAACTTTATCAGGAAATTTGAGATCATTTCATTGACAAATGGAACCTTGCTTTGTTTTCAGGAAGAGCCATTGGAGATAACATGGAAGCACTTGGCCGCTACAGATCGAATTCCACCAGTTTCTCTAATCAAGGAGAGGTTCTGTACAAAGCTCGAGAAGGATGATTATGTTGTTGCTCTCCAATGCATCACAATTAGCACACCAAAAGATTTGCAACCATTTTCAAAGTTGTCATGGTTGAATCTATTCAAAGAAAATTCTCAACGGTTTCAGAAGGATACAGTTGTCAGGCTTATGAATGCAGCCAGTAATGTAATTTCCAATAATAGTGTGCCAAATCCAGCACTTGTTTGTTTAATTCAATCATGCAAAGATTTTTGTTTTGCCACTGATCTCAGTCCAGCTGACATGGATTCAGCAAACAATGTATTTGCATTAGAAAGCAAACAGGAAGTAAACCAATACTAG

Protein sequence

>MsG0480020025.01.T01

MSNFIRKFEIISLTNGTLLCFQEEPLEITWKHLAATDRIPPVSLIKERFCTKLEKDDYVVALQCITISTPKDLQPFSKLSWLNLFKENSQRFQKDTVVRLMNAASNVISNNSVPNPALVCLIQSCKDFCFATDLSPADMDSANNVFALESKQEVNQY*