AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031989.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATATTTAAGCGATTTA+TGG 0.102731 6:-31390464 None:intergenic
TATCATTACCCACCCCTTTA+AGG 0.161706 6:-31390672 None:intergenic
ATCCTTTCCCTTGCTTGCTT+TGG 0.202597 6:-31390755 None:intergenic
GAGGACAAGGTGAGCCTTAA+AGG 0.207697 6:+31390658 MsG0680031989.01.T01:CDS
TCCTTTCCCTTGCTTGCTTT+GGG 0.234394 6:-31390754 None:intergenic
CATGTAATCATTGGTCCTTA+TGG 0.280646 6:-31390783 None:intergenic
CTCGCAGTGGCGAGTTCAAC+GGG 0.290168 6:+31389776 MsG0680031989.01.T01:CDS
GCTCGCAGTGGCGAGCTAAA+AGG 0.299203 6:+31389824 MsG0680031989.01.T01:CDS
GTCTTCCCATGTAGCCTCTA+TGG 0.346024 6:-31390596 None:intergenic
CTGTACCACGAGCCCATTCT+TGG 0.392087 6:-31390545 None:intergenic
ATGTAATCATTGGTCCTTAT+GGG 0.392289 6:-31390782 None:intergenic
AGACAACTCCACACCAAGAA+TGG 0.426453 6:+31390532 MsG0680031989.01.T01:CDS
AGGACAAGGTGAGCCTTAAA+GGG 0.438914 6:+31390659 MsG0680031989.01.T01:CDS
ACACCAACCAATTCACTACT+TGG 0.441940 6:+31390505 MsG0680031989.01.T01:CDS
CTTCGGCCCAGTGTGACTCT+TGG 0.444971 6:-31390720 None:intergenic
TTGTCCTCAAGGTGCACATC+AGG 0.446059 6:-31390643 None:intergenic
TCTTGAGCCAAGTAGTGAAT+TGG 0.449637 6:-31390512 None:intergenic
TGGTACAGGTACTGGTTCAA+TGG 0.450548 6:+31390560 MsG0680031989.01.T01:CDS
CAAGAATGGGCTCGTGGTAC+AGG 0.454234 6:+31390546 MsG0680031989.01.T01:CDS
TTTGGGCTCTTGTTCTCCTT+CGG 0.460481 6:-31390737 None:intergenic
ACAAGAGCCCAAAGCAAGCA+AGG 0.463035 6:+31390747 MsG0680031989.01.T01:CDS
TAAGGCTCACCTTGTCCTCA+AGG 0.471741 6:-31390654 None:intergenic
CTCGCAGTGGCGAGCTAAAA+GGG 0.472214 6:+31389825 MsG0680031989.01.T01:CDS
TGGGCTCGTGGTACAGGTAC+TGG 0.489302 6:+31390552 MsG0680031989.01.T01:CDS
GACAACTCCACACCAAGAAT+GGG 0.503619 6:+31390533 MsG0680031989.01.T01:CDS
GGTACAGGTACTGGTTCAAT+GGG 0.531459 6:+31390561 MsG0680031989.01.T01:CDS
GGTTCAATGGGAAGGACTAC+CGG 0.531727 6:+31390573 MsG0680031989.01.T01:CDS
GCCCAAAGCAAGCAAGGGAA+AGG 0.532244 6:+31390753 MsG0680031989.01.T01:CDS
ACTCGCAGTGGCGAGTTCAA+CGG 0.534736 6:+31389775 MsG0680031989.01.T01:CDS
GGGAAAGGATAGCTCCCATA+AGG 0.538980 6:+31390768 MsG0680031989.01.T01:CDS
TACCGGCCATAGAGGCTACA+TGG 0.541725 6:+31390590 MsG0680031989.01.T01:CDS
TCCCATGTAGCCTCTATGGC+CGG 0.553819 6:-31390592 None:intergenic
CAAGAGCCCAAAGCAAGCAA+GGG 0.574747 6:+31390748 MsG0680031989.01.T01:CDS
ACATCTGCCAAGAGTCACAC+TGG 0.575949 6:+31390713 MsG0680031989.01.T01:CDS
CTCAAGGTGCACATCAGGAT+AGG 0.584309 6:-31390638 None:intergenic
CAAGAGTCACACTGGGCCGA+AGG 0.585922 6:+31390721 MsG0680031989.01.T01:CDS
CCACGAGCCCATTCTTGGTG+TGG 0.594891 6:-31390540 None:intergenic
CAAGGTGAGCCTTAAAGGGG+TGG 0.599270 6:+31390663 MsG0680031989.01.T01:CDS
GAGCTAAAAGGGTTACTCGC+AGG 0.600320 6:+31389836 MsG0680031989.01.T01:CDS
GGAAGGACTACCGGCCATAG+AGG 0.603593 6:+31390582 MsG0680031989.01.T01:CDS
TGATGTGCACCTTGAGGACA+AGG 0.615154 6:+31390645 MsG0680031989.01.T01:CDS
AGAGGCTACATGGGAAGACA+AGG 0.620492 6:+31390600 MsG0680031989.01.T01:CDS
CAGGTACTGGTTCAATGGGA+AGG 0.629392 6:+31390565 MsG0680031989.01.T01:CDS
CTATCCTGATGTGCACCTTG+AGG 0.629585 6:+31390639 MsG0680031989.01.T01:CDS
GCGTACAACGTTGCTCGCAG+TGG 0.632502 6:+31389812 MsG0680031989.01.T01:CDS
AAGGTGAGCCTTAAAGGGGT+GGG 0.635156 6:+31390664 MsG0680031989.01.T01:CDS
AGACAAGGACAAATTACTGA+AGG 0.647869 6:+31390615 MsG0680031989.01.T01:CDS
CATCTGCCAAGAGTCACACT+GGG 0.651439 6:+31390714 MsG0680031989.01.T01:CDS
ACCGGCCATAGAGGCTACAT+GGG 0.651778 6:+31390591 MsG0680031989.01.T01:CDS
CCACACCAAGAATGGGCTCG+TGG 0.680229 6:+31390540 MsG0680031989.01.T01:CDS
GGACAAGGTGAGCCTTAAAG+GGG 0.681321 6:+31390660 MsG0680031989.01.T01:CDS
GAGCCAAGTAGTGAATTGGT+TGG 0.684377 6:-31390508 None:intergenic
GCGAGCTAAACAACTCGCAG+TGG 0.688608 6:+31389763 MsG0680031989.01.T01:CDS
TCGCAGTGGCGAGTTCAACG+GGG 0.757652 6:+31389777 MsG0680031989.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTAATTATGAAAAAG+TGG + Chr6:31390477-31390496 MsG0680031989.01.T01:CDS 10.0%
!! AATATTAATTATGAAAAAGT+GGG + Chr6:31390478-31390497 MsG0680031989.01.T01:CDS 10.0%
!! TAGAAATAATTAAATTACTA+GGG - Chr6:31390328-31390347 None:intergenic 10.0%
!! AGAAATAATTAAATTACTAG+GGG - Chr6:31390327-31390346 None:intergenic 15.0%
!! ATTAATATTTAAGCGATTTA+TGG - Chr6:31390467-31390486 None:intergenic 15.0%
!! GTAGAAATAATTAAATTACT+AGG - Chr6:31390329-31390348 None:intergenic 15.0%
!!! AATATAAGTATTTTCTTAAG+TGG - Chr6:31390371-31390390 None:intergenic 15.0%
!!! ATATAAGTATTTTCTTAAGT+GGG - Chr6:31390370-31390389 None:intergenic 15.0%
!! AAAGAATAAAAGTCTGTAAT+TGG - Chr6:31390270-31390289 None:intergenic 20.0%
!! AAGAATAAAAGTCTGTAATT+GGG - Chr6:31390269-31390288 None:intergenic 20.0%
!!! TATAAGTATTTTCTTAAGTG+GGG - Chr6:31390369-31390388 None:intergenic 20.0%
! AGGTCAAAAATCATTAAAAG+TGG - Chr6:31390050-31390069 None:intergenic 25.0%
! GATTTCAGAATCATAATCTA+AGG - Chr6:31390155-31390174 None:intergenic 25.0%
! TCATTAAAAGTGGAAGAATT+AGG - Chr6:31390040-31390059 None:intergenic 25.0%
!! AGAATCATAATCTAAGGTAA+GGG - Chr6:31390149-31390168 None:intergenic 25.0%
!! ATAAGTATTTTCTTAAGTGG+GGG - Chr6:31390368-31390387 None:intergenic 25.0%
! ATCGATTGTTGTAGGTTTTA+GGG - Chr6:31390082-31390101 None:intergenic 30.0%
! CAGAATCATAATCTAAGGTA+AGG - Chr6:31390150-31390169 None:intergenic 30.0%
AGACAAGGACAAATTACTGA+AGG + Chr6:31390615-31390634 MsG0680031989.01.T01:CDS 35.0%
TAGAAGTGATCGATTGTTGT+AGG - Chr6:31390090-31390109 None:intergenic 35.0%
! GATCGATTGTTGTAGGTTTT+AGG - Chr6:31390083-31390102 None:intergenic 35.0%
!! CTGATTGGTGATGAATTAGA+GGG - Chr6:31389957-31389976 None:intergenic 35.0%
!!! TGTTGTAGGTTTTAGGGATT+GGG - Chr6:31390076-31390095 None:intergenic 35.0%
!!! TTGTTGTAGGTTTTAGGGAT+TGG - Chr6:31390077-31390096 None:intergenic 35.0%
AATTAGAACGCGTACAACAG+AGG - Chr6:31390240-31390259 None:intergenic 40.0%
ACACCAACCAATTCACTACT+TGG + Chr6:31390505-31390524 MsG0680031989.01.T01:CDS 40.0%
GAACACGTTTAGATGCTGAT+TGG - Chr6:31389972-31389991 None:intergenic 40.0%
TATCATTACCCACCCCTTTA+AGG - Chr6:31390675-31390694 None:intergenic 40.0%
TCTTGAGCCAAGTAGTGAAT+TGG - Chr6:31390515-31390534 None:intergenic 40.0%
TGTGGTGAGAAGAGCAATTA+GGG - Chr6:31390293-31390312 None:intergenic 40.0%
TTGTGGTGAGAAGAGCAATT+AGG - Chr6:31390294-31390313 None:intergenic 40.0%
!! GCTGATTGGTGATGAATTAG+AGG - Chr6:31389958-31389977 None:intergenic 40.0%
!!! AGGTTTTAGGGATTGGGATT+AGG - Chr6:31390070-31390089 None:intergenic 40.0%
ACCCTAAAGAACGAAGAACG+CGG - Chr6:31390184-31390203 None:intergenic 45.0%
AGACAACTCCACACCAAGAA+TGG + Chr6:31390532-31390551 MsG0680031989.01.T01:CDS 45.0%
AGGACAAGGTGAGCCTTAAA+GGG + Chr6:31390659-31390678 MsG0680031989.01.T01:CDS 45.0%
ATCCTTTCCCTTGCTTGCTT+TGG - Chr6:31390758-31390777 None:intergenic 45.0%
ATGACAGAACAGGGTTCTGT+CGG + Chr6:31389889-31389908 MsG0680031989.01.T01:intron 45.0%
CTAGGGGAAAGAGTAAGTTG+TGG - Chr6:31390311-31390330 None:intergenic 45.0%
GACAACTCCACACCAAGAAT+GGG + Chr6:31390533-31390552 MsG0680031989.01.T01:CDS 45.0%
GAGCCAAGTAGTGAATTGGT+TGG - Chr6:31390511-31390530 None:intergenic 45.0%
TCCTTTCCCTTGCTTGCTTT+GGG - Chr6:31390757-31390776 None:intergenic 45.0%
TTTGGGCTCTTGTTCTCCTT+CGG - Chr6:31390740-31390759 None:intergenic 45.0%
! TGGTGATGAATTAGAGGGCT+GGG - Chr6:31389952-31389971 None:intergenic 45.0%
!! GGTACAGGTACTGGTTCAAT+GGG + Chr6:31390561-31390580 MsG0680031989.01.T01:CDS 45.0%
!! TCGTTCTTTAGGGTTCTCTC+TGG + Chr6:31390190-31390209 MsG0680031989.01.T01:intron 45.0%
!! TGGTACAGGTACTGGTTCAA+TGG + Chr6:31390560-31390579 MsG0680031989.01.T01:CDS 45.0%
!! TTGGTGATGAATTAGAGGGC+TGG - Chr6:31389953-31389972 None:intergenic 45.0%
!! TATATAGTATATATATATAT+TGG - Chr6:31390410-31390429 None:intergenic 5.0%
AAGGTGAGCCTTAAAGGGGT+GGG + Chr6:31390664-31390683 MsG0680031989.01.T01:CDS 50.0%
ACAAGAGCCCAAAGCAAGCA+AGG + Chr6:31390747-31390766 MsG0680031989.01.T01:CDS 50.0%
ACATCTGCCAAGAGTCACAC+TGG + Chr6:31390713-31390732 MsG0680031989.01.T01:CDS 50.0%
AGAGGCTACATGGGAAGACA+AGG + Chr6:31390600-31390619 MsG0680031989.01.T01:CDS 50.0%
AGCCGCGTTCTTCGTTCTTT+AGG + Chr6:31390179-31390198 MsG0680031989.01.T01:intron 50.0%
CAAGAGCCCAAAGCAAGCAA+GGG + Chr6:31390748-31390767 MsG0680031989.01.T01:CDS 50.0%
CATCTGCCAAGAGTCACACT+GGG + Chr6:31390714-31390733 MsG0680031989.01.T01:CDS 50.0%
CTATCCTGATGTGCACCTTG+AGG + Chr6:31390639-31390658 MsG0680031989.01.T01:CDS 50.0%
CTCAAGGTGCACATCAGGAT+AGG - Chr6:31390641-31390660 None:intergenic 50.0%
GAGCTAAAAGGGTTACTCGC+AGG + Chr6:31389836-31389855 MsG0680031989.01.T01:CDS 50.0%
GAGGACAAGGTGAGCCTTAA+AGG + Chr6:31390658-31390677 MsG0680031989.01.T01:CDS 50.0%
GCCGCGTTCTTCGTTCTTTA+GGG + Chr6:31390180-31390199 MsG0680031989.01.T01:intron 50.0%
GGACAAGGTGAGCCTTAAAG+GGG + Chr6:31390660-31390679 MsG0680031989.01.T01:CDS 50.0%
GGGAAAGGATAGCTCCCATA+AGG + Chr6:31390768-31390787 MsG0680031989.01.T01:CDS 50.0%
GGTTCAATGGGAAGGACTAC+CGG + Chr6:31390573-31390592 MsG0680031989.01.T01:CDS 50.0%
GTCTTCCCATGTAGCCTCTA+TGG - Chr6:31390599-31390618 None:intergenic 50.0%
TAAGGCTCACCTTGTCCTCA+AGG - Chr6:31390657-31390676 None:intergenic 50.0%
TGATGTGCACCTTGAGGACA+AGG + Chr6:31390645-31390664 MsG0680031989.01.T01:CDS 50.0%
TTGCGAGCGATGACAGAACA+GGG + Chr6:31389880-31389899 MsG0680031989.01.T01:intron 50.0%
TTGTCCTCAAGGTGCACATC+AGG - Chr6:31390646-31390665 None:intergenic 50.0%
!! CAGGTACTGGTTCAATGGGA+AGG + Chr6:31390565-31390584 MsG0680031989.01.T01:CDS 50.0%
ACCGGCCATAGAGGCTACAT+GGG + Chr6:31390591-31390610 MsG0680031989.01.T01:CDS 55.0%
ACTCGCAGTGGCGAGTTCAA+CGG + Chr6:31389775-31389794 MsG0680031989.01.T01:CDS 55.0%
AGAACGAAGAACGCGGCTGT+TGG - Chr6:31390177-31390196 None:intergenic 55.0%
CAAGGTGAGCCTTAAAGGGG+TGG + Chr6:31390663-31390682 MsG0680031989.01.T01:CDS 55.0%
CTCGCAGTGGCGAGCTAAAA+GGG + Chr6:31389825-31389844 MsG0680031989.01.T01:CDS 55.0%
CTGTACCACGAGCCCATTCT+TGG - Chr6:31390548-31390567 None:intergenic 55.0%
GCCCAAAGCAAGCAAGGGAA+AGG + Chr6:31390753-31390772 MsG0680031989.01.T01:CDS 55.0%
GCGAGCTAAACAACTCGCAG+TGG + Chr6:31389763-31389782 MsG0680031989.01.T01:CDS 55.0%
GTTGCGAGCGATGACAGAAC+AGG + Chr6:31389879-31389898 MsG0680031989.01.T01:intron 55.0%
TACCGGCCATAGAGGCTACA+TGG + Chr6:31390590-31390609 MsG0680031989.01.T01:CDS 55.0%
TCCCATGTAGCCTCTATGGC+CGG - Chr6:31390595-31390614 None:intergenic 55.0%
! CAAGAATGGGCTCGTGGTAC+AGG + Chr6:31390546-31390565 MsG0680031989.01.T01:CDS 55.0%
CAAGAGTCACACTGGGCCGA+AGG + Chr6:31390721-31390740 MsG0680031989.01.T01:CDS 60.0%
CCACGAGCCCATTCTTGGTG+TGG - Chr6:31390543-31390562 None:intergenic 60.0%
CTCGCAGTGGCGAGTTCAAC+GGG + Chr6:31389776-31389795 MsG0680031989.01.T01:CDS 60.0%
CTTCGGCCCAGTGTGACTCT+TGG - Chr6:31390723-31390742 None:intergenic 60.0%
GCGTACAACGTTGCTCGCAG+TGG + Chr6:31389812-31389831 MsG0680031989.01.T01:CDS 60.0%
GCTCGCAGTGGCGAGCTAAA+AGG + Chr6:31389824-31389843 MsG0680031989.01.T01:CDS 60.0%
GGAAGGACTACCGGCCATAG+AGG + Chr6:31390582-31390601 MsG0680031989.01.T01:CDS 60.0%
GGTTACTCGCAGGTGCGAGT+AGG + Chr6:31389846-31389865 MsG0680031989.01.T01:intron 60.0%
TCGCAGTGGCGAGTTCAACG+GGG + Chr6:31389777-31389796 MsG0680031989.01.T01:CDS 60.0%
TGGGCTCGTGGTACAGGTAC+TGG + Chr6:31390552-31390571 MsG0680031989.01.T01:CDS 60.0%
! CCACACCAAGAATGGGCTCG+TGG + Chr6:31390540-31390559 MsG0680031989.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 31389761 31390798 31389761 ID=MsG0680031989.01;Name=MsG0680031989.01
Chr6 mRNA 31389761 31390798 31389761 ID=MsG0680031989.01.T01;Parent=MsG0680031989.01;Name=MsG0680031989.01.T01;_AED=0.72;_eAED=0.74;_QI=0|0|0|0.5|0|0|2|0|143
Chr6 exon 31389761 31389857 31389761 ID=MsG0680031989.01.T01:exon:15366;Parent=MsG0680031989.01.T01
Chr6 exon 31390464 31390798 31390464 ID=MsG0680031989.01.T01:exon:15367;Parent=MsG0680031989.01.T01
Chr6 CDS 31389761 31389857 31389761 ID=MsG0680031989.01.T01:cds;Parent=MsG0680031989.01.T01
Chr6 CDS 31390464 31390798 31390464 ID=MsG0680031989.01.T01:cds;Parent=MsG0680031989.01.T01
Gene Sequence

>MsG0680031989.01.T01

TTGCGAGCTAAACAACTCGCAGTGGCGAGTTCAACGGGGCAACTCGCAGTTGCGTACAACGTTGCTCGCAGTGGCGAGCTAAAAGGGTTACTCGCAGCCATAAATCGCTTAAATATTAATTATGAAAAAGTGGGCGTTACACCAACCAATTCACTACTTGGCTCAAGACAACTCCACACCAAGAATGGGCTCGTGGTACAGGTACTGGTTCAATGGGAAGGACTACCGGCCATAGAGGCTACATGGGAAGACAAGGACAAATTACTGAAGGCCTATCCTGATGTGCACCTTGAGGACAAGGTGAGCCTTAAAGGGGTGGGTAATGATACGCATGTGTTCAATAACAACATCTGCCAAGAGTCACACTGGGCCGAAGGAGAACAAGAGCCCAAAGCAAGCAAGGGAAAGGATAGCTCCCATAAGGACCAATGA

Protein sequence

>MsG0680031989.01.T01

LRAKQLAVASSTGQLAVAYNVARSGELKGLLAAINRLNINYEKVGVTPTNSLLGSRQLHTKNGLVVQVLVQWEGLPAIEATWEDKDKLLKAYPDVHLEDKVSLKGVGNDTHVFNNNICQESHWAEGEQEPKASKGKDSSHKDQ*