Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033368.01.T01 | XP_013442481.1 | 94.118 | 510 | 28 | 1 | 1 | 508 | 1 | 510 | 0 | 947 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033368.01.T01 | A0A072TH15 | 94.118 | 510 | 28 | 1 | 1 | 508 | 1 | 510 | 0.0 | 947 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0680033368.01 | 0.804439 | 2.158615e-49 | 1.113003e-46 |
| MsG0180003320.01 | MsG0680033368.01 | 0.822922 | 1.827691e-53 | 1.550993e-50 |
| MsG0280009232.01 | MsG0680033368.01 | 0.808727 | 2.686938e-50 | 1.547884e-47 |
| MsG0280011046.01 | MsG0680033368.01 | 0.804723 | 1.883748e-49 | 9.785015e-47 |
| MsG0380013263.01 | MsG0680033368.01 | 0.821061 | 4.933384e-53 | 3.972076e-50 |
| MsG0380017168.01 | MsG0680033368.01 | 0.827622 | 1.413066e-54 | 1.371321e-51 |
| MsG0380017901.01 | MsG0680033368.01 | 0.807884 | 4.064758e-50 | 2.290361e-47 |
| MsG0480019024.01 | MsG0680033368.01 | 0.843796 | 1.124006e-58 | 1.778425e-55 |
| MsG0480020118.01 | MsG0680033368.01 | 0.850358 | 1.788716e-60 | 3.494435e-57 |
| MsG0480020904.01 | MsG0680033368.01 | 0.826106 | 3.253579e-54 | 3.022579e-51 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033368.01.T01 | MTR_0161s0050 | 94.118 | 510 | 28 | 1 | 1 | 508 | 1 | 510 | 0.0 | 947 |
| MsG0680033368.01.T01 | MTR_5g076200 | 90.551 | 508 | 48 | 0 | 1 | 508 | 1 | 508 | 0.0 | 936 |
| MsG0680033368.01.T01 | MTR_5g076220 | 87.205 | 508 | 65 | 0 | 1 | 508 | 1 | 508 | 0.0 | 909 |
| MsG0680033368.01.T01 | MTR_5g076240 | 65.234 | 512 | 177 | 1 | 1 | 512 | 1 | 511 | 0.0 | 723 |
| MsG0680033368.01.T01 | MTR_8g074040 | 51.262 | 515 | 231 | 7 | 1 | 508 | 43 | 544 | 6.18e-177 | 511 |
| MsG0680033368.01.T01 | MTR_5g008800 | 50.680 | 515 | 238 | 8 | 1 | 511 | 1 | 503 | 1.17e-174 | 503 |
| MsG0680033368.01.T01 | MTR_3g064420 | 49.623 | 530 | 236 | 7 | 1 | 512 | 1 | 517 | 2.16e-173 | 501 |
| MsG0680033368.01.T01 | MTR_5g008820 | 48.538 | 513 | 250 | 6 | 1 | 511 | 1 | 501 | 1.17e-167 | 485 |
| MsG0680033368.01.T01 | MTR_2g044180 | 48.263 | 518 | 253 | 7 | 1 | 508 | 7 | 519 | 2.14e-166 | 483 |
| MsG0680033368.01.T01 | MTR_4g121600 | 47.409 | 521 | 253 | 7 | 1 | 508 | 7 | 519 | 1.47e-162 | 473 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033368.01.T01 | AT5G16370 | 62.963 | 513 | 182 | 6 | 1 | 511 | 1 | 507 | 0.0 | 666 |
| MsG0680033368.01.T01 | AT5G16340 | 62.768 | 513 | 183 | 6 | 1 | 511 | 1 | 507 | 0.0 | 659 |
| MsG0680033368.01.T01 | AT1G21540 | 61.719 | 512 | 189 | 5 | 1 | 508 | 1 | 509 | 0.0 | 644 |
| MsG0680033368.01.T01 | AT1G75960 | 60.861 | 511 | 190 | 6 | 1 | 508 | 1 | 504 | 0.0 | 632 |
| MsG0680033368.01.T01 | AT1G21530 | 61.089 | 514 | 188 | 7 | 1 | 508 | 1 | 508 | 0.0 | 630 |
| MsG0680033368.01.T01 | AT1G21530 | 60.895 | 514 | 187 | 8 | 1 | 508 | 1 | 506 | 0.0 | 623 |
| MsG0680033368.01.T01 | AT1G77240 | 58.513 | 511 | 202 | 5 | 1 | 508 | 1 | 504 | 0.0 | 620 |
| MsG0680033368.01.T01 | AT1G66120 | 50.097 | 517 | 240 | 8 | 1 | 508 | 1 | 508 | 4.20e-176 | 508 |
| MsG0680033368.01.T01 | AT1G20560 | 50.201 | 498 | 242 | 4 | 1 | 495 | 3 | 497 | 7.02e-170 | 491 |
| MsG0680033368.01.T01 | AT1G65880 | 48.560 | 521 | 248 | 8 | 1 | 512 | 1 | 510 | 2.34e-169 | 491 |
Find 154 sgRNAs with CRISPR-Local
Find 187 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATGAGAATGCCACGT+TGG | 0.673283 | 6:-63273687 | MsG0680033368.01.T01:CDS |
| AAACATTATGAACACCTACG+AGG | 0.706599 | 6:-63274419 | MsG0680033368.01.T01:CDS |
| AAAGATGTGATAATAAGTGG+TGG | 0.597902 | 6:-63273578 | MsG0680033368.01.T01:CDS |
| AAAGGTGCTTGTGTTATGTT+AGG | 0.422849 | 6:-63273716 | MsG0680033368.01.T01:CDS |
| AAATGATATCCCTACTGAAA+AGG | 0.485535 | 6:-63273414 | MsG0680033368.01.T01:CDS |
| AACATTATGAACACCTACGA+GGG | 0.661878 | 6:-63274418 | MsG0680033368.01.T01:CDS |
| AACGGATGGAGCTATCCATG+GGG | 0.749202 | 6:-63274181 | MsG0680033368.01.T01:CDS |
| AACTGAAGCTCGTACATGGC+CGG | 0.512459 | 6:+63274679 | None:intergenic |
| AAGAAGGTGAGTGGGGTTAG+AGG | 0.380690 | 6:+63274865 | None:intergenic |
| AAGATGTGATAATAAGTGGT+GGG | 0.572024 | 6:-63273577 | MsG0680033368.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATGTGATAATAAGTGG+TGG | - | Chr6:63274699-63274718 | MsG0680033368.01.T01:CDS | 30.0% | |
| AAATGATATCCCTACTGAAA+AGG | - | Chr6:63274863-63274882 | MsG0680033368.01.T01:CDS | 30.0% | |
| AAGATGTGATAATAAGTGGT+GGG | - | Chr6:63274700-63274719 | MsG0680033368.01.T01:CDS | 30.0% | |
| AAGTTGAAGACATCGTTTAT+GGG | + | Chr6:63273512-63273531 | None:intergenic | 30.0% | |
| AGGGTGATCTAAATTTCAAA+TGG | - | Chr6:63273893-63273912 | MsG0680033368.01.T01:CDS | 30.0% | |
| GAGTTTAAAGGATGAATTGA+AGG | - | Chr6:63274839-63274858 | MsG0680033368.01.T01:CDS | 30.0% | |
| GCTGTTGTAAATGATTGAAA+TGG | + | Chr6:63273467-63273486 | None:intergenic | 30.0% | |
| TCAATTAAACTGTAGATCGT+TGG | + | Chr6:63274171-63274190 | None:intergenic | 30.0% | |
| TTGCAGATGTTTCATTCTAA+CGG | - | Chr6:63274078-63274097 | MsG0680033368.01.T01:CDS | 30.0% | |
| AAACATTATGAACACCTACG+AGG | - | Chr6:63273858-63273877 | MsG0680033368.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 63273376 | 63274923 | 63273376 | ID=MsG0680033368.01;Name=MsG0680033368.01 |
| Chr6 | mRNA | 63273376 | 63274923 | 63273376 | ID=MsG0680033368.01.T01;Parent=MsG0680033368.01;Name=MsG0680033368.01.T01;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|515 |
| Chr6 | exon | 63273376 | 63274923 | 63273376 | ID=MsG0680033368.01.T01:exon:4118;Parent=MsG0680033368.01.T01 |
| Chr6 | CDS | 63273376 | 63274923 | 63273376 | ID=MsG0680033368.01.T01:cds;Parent=MsG0680033368.01.T01 |
| Gene Sequence |
>MsG0680033368.01.T01 ATGAACCAGTTAACCAAAAACCAAGCAAACTCGACACCTCTAACCCCACTCACCTTCTTAGACAGAGCAGCCATTGTCTATGGCAACTCCATTTCAATCATTTACAACAGCACTTCATTCACATGGTTTCAAACCCATAAACGATGTCTTCAACTTGCTTCATCACTTTCTTCCCTCGGCATCGAAAAAGGCGACGTTGTCTCCGTCCTCTCTCCCAACACTCCGGCCATGTACGAGCTTCAGTTCTCCGTTCCAATGTCCGGCGCCATTCTCAACAACCTCAACTTCCGACTTGACTACAAAACCCTCTCCGTGCTGCTCATCCACAGTGAATCGAAACTCATCTTTGTTGACATTCTCTCCCTTTCTCTTGCCCTCAACGCTCTTTCCTTATTCCCCAAAAAGACTCCAAGCCCTAAGCTCGTCCTCATCATGGATGAAACCCTAATGTCGCACCAGATTCCACCACTTCCAAAAAACATAAACATTATGAACACCTACGAGGGTCTTGTAGCGAAGGGTGATCTAAATTTCAAATGGATCCGTCCCGACACCGAGTGGGATCCGATTACGCTGAACTACACCTCCGGAACAACGTCGTCTCCAAAAGGTGTAGTGCATTGTCACAGAGCAACGTTCATTGTTTCACTTGATTCTCTCATTGATTGGTCGGTACCGGTTCAACCAGTTTTCTTGTGGACGTTGCAGATGTTTCATTCTAACGGATGGAGCTATCCATGGGGAATGGCTGCCGTCGGAGGAACGAATATCTGCACACGTAGGACCGATGCGCCAACGATCTACAGTTTAATTGAATCTCATGGTGTAACACACATGTGTGCTGCACCAGTTGTACTTAACATGCTGTCAAATTTCAACAAAAATGAACCGTTAAAAAAACCGGTTCATGTTTTGACAGGAGGGTCATCGCCACCAGCAGCGATTCTCACACGAGCAGAGAAGTTAGGGTTTGAGGTTAGTCATGGTTATGGAATGACGGAGGTAATTGGGGTGATTGTGTCGTGTGCTTGGAAAAGGGAATGGGATAGGTTTCCGGCGACAGGGAAGGCGAGGATGAAGGCGAGGCAAGGTGTGAGGAGGGTAGGTGTGATGGAGGTTGATGTGGTGGGACCCACCGGAGAAAGTGTTAAGCACGATGGTGTAACGGTTGGTGAGATAGTTGTAAAAGGTGCTTGTGTTATGTTAGGTTATTTAAAAGATGAGAATGCCACGTTGGAGTGTATGAAAAATAATGGTTGGTTTTATACGGGTGATGTTGCGGTTATGCATGAGGATGGGTATTTGGAGATTAAGGATAGGTCAAAAGATGTGATAATAAGTGGTGGGGAGAATATGAGTAGTGTGGAAGTTGAGGGTGTTTTGTACATGCATCCCGCGGTTAAGGAGGCGGCGGTGGTGGCGAGGCCGGATGATTTTTGGGGAGAGACGCCGTGTGCGTTTGTGAGTTTAAAGGATGAATTGAAGGAAAATGATATCCCTACTGAAAAGGAGATTATGAAGTTTAATATCAAGGACTATTTTGACTAA |
| Protein sequence |
>MsG0680033368.01.T01 MNQLTKNQANSTPLTPLTFLDRAAIVYGNSISIIYNSTSFTWFQTHKRCLQLASSLSSLGIEKGDVVSVLSPNTPAMYELQFSVPMSGAILNNLNFRLDYKTLSVLLIHSESKLIFVDILSLSLALNALSLFPKKTPSPKLVLIMDETLMSHQIPPLPKNINIMNTYEGLVAKGDLNFKWIRPDTEWDPITLNYTSGTTSSPKGVVHCHRATFIVSLDSLIDWSVPVQPVFLWTLQMFHSNGWSYPWGMAAVGGTNICTRRTDAPTIYSLIESHGVTHMCAAPVVLNMLSNFNKNEPLKKPVHVLTGGSSPPAAILTRAEKLGFEVSHGYGMTEVIGVIVSCAWKREWDRFPATGKARMKARQGVRRVGVMEVDVVGPTGESVKHDGVTVGEIVVKGACVMLGYLKDENATLECMKNNGWFYTGDVAVMHEDGYLEIKDRSKDVIISGGENMSSVEVEGVLYMHPAVKEAAVVARPDDFWGETPCAFVSLKDELKENDIPTEKEIMKFNIKDYFD* |
