Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013263.01.T01 | XP_013459385.1 | 84.158 | 101 | 0 | 1 | 8 | 108 | 8 | 92 | 4.06E-47 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013263.01.T01 | Q9CA64 | 64.516 | 93 | 16 | 2 | 17 | 108 | 17 | 93 | 2.12E-30 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013263.01.T01 | I3STA1 | 84.158 | 101 | 0 | 1 | 8 | 108 | 8 | 92 | 1.94e-47 | 156 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002238.01 | MsG0380013263.01 | 0.808768 | 2.633412e-50 | 1.518668e-47 |
| MsG0180003320.01 | MsG0380013263.01 | 0.826249 | 3.008965e-54 | 2.806763e-51 |
| MsG0380013263.01 | MsG0380016490.01 | 0.805744 | 1.151236e-49 | 6.139218e-47 |
| MsG0380013263.01 | MsG0380017168.01 | 0.806975 | 6.334745e-50 | 3.486143e-47 |
| MsG0380013263.01 | MsG0480019024.01 | 0.810682 | 1.021071e-50 | 6.195143e-48 |
| MsG0380013263.01 | MsG0480020118.01 | 0.850236 | 1.934446e-60 | 3.764616e-57 |
| MsG0380013263.01 | MsG0480021091.01 | 0.812030 | 5.205411e-51 | 3.273134e-48 |
| MsG0380013263.01 | MsG0480022055.01 | 0.809137 | 2.195070e-50 | 1.278538e-47 |
| MsG0380013263.01 | MsG0480022886.01 | 0.804881 | 1.745649e-49 | 9.105117e-47 |
| MsG0380013263.01 | MsG0580026521.01 | 0.824089 | 9.751129e-54 | 8.551441e-51 |
| MsG0380013263.01 | MsG0680031200.01 | 0.804853 | 1.769843e-49 | 9.224171e-47 |
| MsG0380013263.01 | MsG0680033367.01 | 0.803112 | 4.071178e-49 | 2.028736e-46 |
| MsG0380013263.01 | MsG0680033368.01 | 0.821061 | 4.933384e-53 | 3.972076e-50 |
| MsG0380013263.01 | MsG0680034418.01 | 0.810132 | 1.341888e-50 | 8.023998e-48 |
| MsG0380013263.01 | MsG0780035930.01 | 0.815228 | 1.029463e-51 | 7.056553e-49 |
| MsG0380013263.01 | MsG0780036259.01 | 0.807692 | 4.463436e-50 | 2.502479e-47 |
| MsG0380013263.01 | MsG0780038657.01 | 0.868419 | 6.624412e-66 | 2.394246e-62 |
| MsG0380013263.01 | MsG0780039373.01 | 0.802976 | 4.344815e-49 | 2.157572e-46 |
| MsG0380013263.01 | MsG0780040497.01 | 0.810568 | 1.080231e-50 | 6.534027e-48 |
| MsG0380013263.01 | MsG0780040716.01 | 0.816850 | 4.471171e-52 | 3.204155e-49 |
| MsG0280007909.01 | MsG0380013263.01 | 0.821852 | 3.238675e-53 | 2.665971e-50 |
| MsG0280009232.01 | MsG0380013263.01 | 0.821455 | 4.000890e-53 | 3.256401e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013263.01.T01 | MTR_3g438070 | 84.158 | 101 | 0 | 1 | 8 | 108 | 8 | 92 | 4.91e-51 | 156 |
| MsG0380013263.01.T01 | MTR_7g010120 | 56.881 | 109 | 29 | 2 | 2 | 108 | 1 | 93 | 1.34e-30 | 104 |
| MsG0380013263.01.T01 | MTR_5g039180 | 60.215 | 93 | 19 | 2 | 17 | 107 | 17 | 93 | 9.25e-29 | 100 |
| MsG0380013263.01.T01 | MTR_6g011670 | 60.638 | 94 | 19 | 2 | 17 | 108 | 15 | 92 | 3.57e-28 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013263.01.T01 | AT1G74500 | 64.516 | 93 | 16 | 2 | 17 | 108 | 17 | 93 | 2.16e-31 | 106 |
| MsG0380013263.01.T01 | AT3G47710 | 60.784 | 102 | 22 | 3 | 8 | 108 | 8 | 92 | 3.21e-30 | 103 |
| MsG0380013263.01.T01 | AT1G26945 | 65.556 | 90 | 13 | 2 | 19 | 106 | 19 | 92 | 3.83e-30 | 103 |
| MsG0380013263.01.T01 | AT3G28857 | 55.140 | 107 | 29 | 3 | 4 | 107 | 2 | 92 | 4.21e-28 | 98.6 |
| MsG0380013263.01.T01 | AT5G39860 | 57.447 | 94 | 21 | 3 | 17 | 107 | 15 | 92 | 1.31e-25 | 92.0 |
| MsG0380013263.01.T01 | AT5G15160 | 54.945 | 91 | 22 | 3 | 19 | 106 | 18 | 92 | 7.22e-23 | 85.1 |
Find 17 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCGAGGGTGTTGC+AGG | 0.348972 | 3:-33981661 | MsG0380013263.01.T01:CDS |
| TGAGCGTTTGTCTGAGCTAT+TGG | 0.392203 | 3:-33981589 | MsG0380013263.01.T01:CDS |
| ACTAGGAAACAAATAATTGT+AGG | 0.459750 | 3:+33982488 | None:intergenic |
| AGGAAGAAGTTGCTGCAACT+TGG | 0.461097 | 3:+33982545 | None:intergenic |
| GAGCTCCTATTGCGAATCTC+AGG | 0.466230 | 3:+33982525 | None:intergenic |
| TATATCAGGTTTCAGCTTCG+AGG | 0.481740 | 3:-33981671 | MsG0380013263.01.T01:intron |
| TTCTTCCTGAGATTCGCAAT+AGG | 0.484861 | 3:-33982530 | MsG0380013263.01.T01:CDS |
| AGATGTCTAGCAGGAGGTCG+CGG | 0.487137 | 3:-33982627 | MsG0380013263.01.T01:CDS |
| TAGCAGGAGGTCGCGGGTCA+CGG | 0.488562 | 3:-33982620 | MsG0380013263.01.T01:CDS |
| ATATCAGGTTTCAGCTTCGA+GGG | 0.550730 | 3:-33981670 | MsG0380013263.01.T01:intron |
| GCTCAGACAAACGCTCACTT+AGG | 0.559789 | 3:+33981595 | None:intergenic |
| GCAACTTGGAGACAAGATCA+TGG | 0.561243 | 3:+33982559 | None:intergenic |
| TCGCAATAGGAGCTCTGACA+AGG | 0.574019 | 3:-33982517 | MsG0380013263.01.T01:CDS |
| CACGGCAAACAAGTAGTTCG+AGG | 0.587225 | 3:-33982602 | MsG0380013263.01.T01:CDS |
| CGCAATAGGAGCTCTGACAA+GGG | 0.589374 | 3:-33982516 | MsG0380013263.01.T01:CDS |
| AAGATCATGGATCTGATCGT+CGG | 0.643584 | 3:+33982572 | None:intergenic |
| TTATAAAGATGTCTAGCAGG+AGG | 0.648667 | 3:-33982633 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTATTAAAATATAGTATA+TGG | + | Chr3:33981888-33981907 | None:intergenic | 10.0% |
| !!! | ATGACTTTTATAAAAAATAA+TGG | + | Chr3:33981794-33981813 | None:intergenic | 10.0% |
| !!! | TTATTTTTTATAAAAGTCAT+TGG | - | Chr3:33981794-33981813 | MsG0380013263.01.T01:intron | 10.0% |
| !! | AAGAAAAAAAAAAACTTGAA+AGG | - | Chr3:33982207-33982226 | MsG0380013263.01.T01:intron | 15.0% |
| !!! | AAATTGAATTTTACTATATC+AGG | - | Chr3:33982475-33982494 | MsG0380013263.01.T01:CDS | 15.0% |
| ! | ACTAGGAAACAAATAATTGT+AGG | + | Chr3:33981675-33981694 | None:intergenic | 25.0% |
| ! | ATTTAAACCAACTTAATCCT+AGG | - | Chr3:33982295-33982314 | MsG0380013263.01.T01:intron | 25.0% |
| ! | GATAATGCATAATATATGCA+GGG | - | Chr3:33982113-33982132 | MsG0380013263.01.T01:intron | 25.0% |
| ! | TGAAAACAATTCTTGTAGAT+GGG | + | Chr3:33982406-33982425 | None:intergenic | 25.0% |
| ! | TTGAAAACAATTCTTGTAGA+TGG | + | Chr3:33982407-33982426 | None:intergenic | 25.0% |
| !!! | AGAAGAGATTAGTTTTATAG+CGG | - | Chr3:33981908-33981927 | MsG0380013263.01.T01:intron | 25.0% |
| ACACTAGAGTAAGTATAAGA+GGG | - | Chr3:33982271-33982290 | MsG0380013263.01.T01:intron | 30.0% | |
| ACCATACTTATTCACCTTTA+AGG | - | Chr3:33982157-33982176 | MsG0380013263.01.T01:intron | 30.0% | |
| ACCTTAAAGGTGAATAAGTA+TGG | + | Chr3:33982161-33982180 | None:intergenic | 30.0% | |
| GGATAATGCATAATATATGC+AGG | - | Chr3:33982112-33982131 | MsG0380013263.01.T01:intron | 30.0% | |
| TACACTAGAGTAAGTATAAG+AGG | - | Chr3:33982270-33982289 | MsG0380013263.01.T01:intron | 30.0% | |
| !! | CTAGTCTTTTGATTAAGACT+AGG | + | Chr3:33981692-33981711 | None:intergenic | 30.0% |
| CAAGAAAAAGGAACAACAAC+AGG | - | Chr3:33982331-33982350 | MsG0380013263.01.T01:intron | 35.0% | |
| GAGATACCCTATTAATCTAC+CGG | - | Chr3:33981952-33981971 | MsG0380013263.01.T01:intron | 35.0% | |
| GAGCTTAGTTCATTTGGTAA+GGG | - | Chr3:33982091-33982110 | MsG0380013263.01.T01:intron | 35.0% | |
| TCTTGTAGATGGGAAAAAAC+TGG | + | Chr3:33982396-33982415 | None:intergenic | 35.0% | |
| TGAGCTTAGTTCATTTGGTA+AGG | - | Chr3:33982090-33982109 | MsG0380013263.01.T01:intron | 35.0% | |
| TTAATCCCCTCGTATTTGTT+AGG | + | Chr3:33982357-33982376 | None:intergenic | 35.0% | |
| ! | TTCTTCTTGTCAATTAGCCT+AGG | + | Chr3:33982192-33982211 | None:intergenic | 35.0% |
| !! | TTATTCACCTTTAAGGTCTC+TGG | - | Chr3:33982164-33982183 | MsG0380013263.01.T01:intron | 35.0% |
| AACAGGCCTAACAAATACGA+GGG | - | Chr3:33982348-33982367 | MsG0380013263.01.T01:intron | 40.0% | |
| AAGATCATGGATCTGATCGT+CGG | + | Chr3:33981591-33981610 | None:intergenic | 40.0% | |
| AGCTCAGAAGGACTTCTTTA+CGG | + | Chr3:33982076-33982095 | None:intergenic | 40.0% | |
| AGGTGAATAAGTATGGTGTC+TGG | + | Chr3:33982154-33982173 | None:intergenic | 40.0% | |
| ATATCAGGTTTCAGCTTCGA+GGG | - | Chr3:33982490-33982509 | MsG0380013263.01.T01:CDS | 40.0% | |
| CCAAATGAACTAAGCTCAGA+AGG | + | Chr3:33982088-33982107 | None:intergenic | 40.0% | |
| CCAGGGACATAACAAGAAAA+AGG | - | Chr3:33982319-33982338 | MsG0380013263.01.T01:intron | 40.0% | |
| CCTTCTGAGCTTAGTTCATT+TGG | - | Chr3:33982085-33982104 | MsG0380013263.01.T01:intron | 40.0% | |
| GACTCTCCGGTAGATTAATA+GGG | + | Chr3:33981961-33981980 | None:intergenic | 40.0% | |
| GGTGAATAAGTATGGTGTCT+GGG | + | Chr3:33982153-33982172 | None:intergenic | 40.0% | |
| GTGAATAAGTATGGTGTCTG+GGG | + | Chr3:33982152-33982171 | None:intergenic | 40.0% | |
| TATATCAGGTTTCAGCTTCG+AGG | - | Chr3:33982489-33982508 | MsG0380013263.01.T01:CDS | 40.0% | |
| TGACTCTCCGGTAGATTAAT+AGG | + | Chr3:33981962-33981981 | None:intergenic | 40.0% | |
| TTCTTCCTGAGATTCGCAAT+AGG | - | Chr3:33981630-33981649 | MsG0380013263.01.T01:CDS | 40.0% | |
| !! | CCTTTTTCTTGTTATGTCCC+TGG | + | Chr3:33982322-33982341 | None:intergenic | 40.0% |
| !! | GAGCTGTTTTGTGAGCTTTT+AGG | + | Chr3:33982004-33982023 | None:intergenic | 40.0% |
| ACAGGCCTAACAAATACGAG+GGG | - | Chr3:33982349-33982368 | MsG0380013263.01.T01:intron | 45.0% | |
| ACCAACTTAATCCTAGGTCC+AGG | - | Chr3:33982301-33982320 | MsG0380013263.01.T01:intron | 45.0% | |
| AGGAAGAAGTTGCTGCAACT+TGG | + | Chr3:33981618-33981637 | None:intergenic | 45.0% | |
| CAACAGGCCTAACAAATACG+AGG | - | Chr3:33982347-33982366 | MsG0380013263.01.T01:intron | 45.0% | |
| CCAACTTAATCCTAGGTCCA+GGG | - | Chr3:33982302-33982321 | MsG0380013263.01.T01:intron | 45.0% | |
| GCAACTTGGAGACAAGATCA+TGG | + | Chr3:33981604-33981623 | None:intergenic | 45.0% | |
| TGAGCGTTTGTCTGAGCTAT+TGG | - | Chr3:33982571-33982590 | MsG0380013263.01.T01:CDS | 45.0% | |
| ! | AGAGTCAGCTCTAACTATGG+TGG | - | Chr3:33981975-33981994 | MsG0380013263.01.T01:intron | 45.0% |
| ! | CATAGTTAGAGCTGACTCTC+CGG | + | Chr3:33981974-33981993 | None:intergenic | 45.0% |
| CACGGCAAACAAGTAGTTCG+AGG | - | Chr3:33981558-33981577 | MsG0380013263.01.T01:CDS | 50.0% | |
| CCCTGGACCTAGGATTAAGT+TGG | + | Chr3:33982305-33982324 | None:intergenic | 50.0% | |
| GAGCTCCTATTGCGAATCTC+AGG | + | Chr3:33981638-33981657 | None:intergenic | 50.0% | |
| GCTCAGACAAACGCTCACTT+AGG | + | Chr3:33982568-33982587 | None:intergenic | 50.0% | |
| ! | CGCAATAGGAGCTCTGACAA+GGG | - | Chr3:33981644-33981663 | MsG0380013263.01.T01:CDS | 50.0% |
| ! | CGGAGAGTCAGCTCTAACTA+TGG | - | Chr3:33981972-33981991 | MsG0380013263.01.T01:intron | 50.0% |
| ! | CTTGTTATGTCCCTGGACCT+AGG | + | Chr3:33982315-33982334 | None:intergenic | 50.0% |
| ! | TCGCAATAGGAGCTCTGACA+AGG | - | Chr3:33981643-33981662 | MsG0380013263.01.T01:CDS | 50.0% |
| CTAGGCGCCAGAGACCTTAA+AGG | + | Chr3:33982174-33982193 | None:intergenic | 55.0% | |
| !! | CTTTAAGGTCTCTGGCGCCT+AGG | - | Chr3:33982172-33982191 | MsG0380013263.01.T01:intron | 55.0% |
| !! | TTCAGCTTCGAGGGTGTTGC+AGG | - | Chr3:33982499-33982518 | MsG0380013263.01.T01:CDS | 55.0% |
| ! | GGTGTCTGGGGTTCGAACTC+TGG | + | Chr3:33982140-33982159 | None:intergenic | 60.0% |
| TAGCAGGAGGTCGCGGGTCA+CGG | - | Chr3:33981540-33981559 | MsG0380013263.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 33981536 | 33982646 | 33981536 | ID=MsG0380013263.01;Name=MsG0380013263.01 |
| Chr3 | mRNA | 33981536 | 33982646 | 33981536 | ID=MsG0380013263.01.T01;Parent=MsG0380013263.01;Name=MsG0380013263.01.T01;_AED=0.03;_eAED=0.07;_QI=0|0|0|1|0|0|2|0|108 |
| Chr3 | exon | 33982470 | 33982646 | 33982470 | ID=MsG0380013263.01.T01:exon:5985;Parent=MsG0380013263.01.T01 |
| Chr3 | exon | 33981536 | 33981685 | 33981536 | ID=MsG0380013263.01.T01:exon:5986;Parent=MsG0380013263.01.T01 |
| Chr3 | CDS | 33982470 | 33982646 | 33982470 | ID=MsG0380013263.01.T01:cds;Parent=MsG0380013263.01.T01 |
| Chr3 | CDS | 33981536 | 33981685 | 33981536 | ID=MsG0380013263.01.T01:cds;Parent=MsG0380013263.01.T01 |
| Gene Sequence |
| Protein sequence |
