AlfalfaGEDB | MsG0480019024.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019024.01

KEGG: -
GO: GO:0016747
PFAM: PF02458

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019024.01.T01	XP_003604963.1	97.674	43	1	0	25	67	45	87	2.31E-19	94.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019024.01.T01	G7JUI6	97.674	43	1	0	25	67	45	87	1.10e-19	94.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048134.01	MsG0480019024.01	0.814605	1.415296e-51	9.539345e-49
MsG0080048509.01	MsG0480019024.01	0.823444	1.380467e-53	1.188790e-50
MsG0180002364.01	MsG0480019024.01	0.804442	2.155831e-49	1.111644e-46
MsG0180003320.01	MsG0480019024.01	0.862570	4.616437e-64	1.358107e-60
MsG0380013263.01	MsG0480019024.01	0.810682	1.021071e-50	6.195143e-48
MsG0480018559.01	MsG0480019024.01	0.806631	7.490587e-50	4.085478e-47
MsG0480018622.01	MsG0480019024.01	0.826925	2.075822e-54	1.974326e-51
MsG0480018625.01	MsG0480019024.01	0.823511	1.331871e-53	1.149158e-50
MsG0480019023.01	MsG0480019024.01	0.868597	5.801689e-66	2.110794e-62
MsG0480019024.01	MsG0480020118.01	0.848127	7.468325e-60	1.356709e-56
MsG0480019024.01	MsG0480020904.01	0.839816	1.263238e-57	1.765012e-54
MsG0480019024.01	MsG0480021129.01	0.831904	1.281640e-55	1.410308e-52
MsG0480019024.01	MsG0480021133.01	0.812183	4.819157e-51	3.043129e-48
MsG0480019024.01	MsG0480021551.01	0.800233	1.586722e-48	7.349762e-46
MsG0480019024.01	MsG0480022886.01	0.808960	2.395869e-50	1.388795e-47
MsG0480019024.01	MsG0580025432.01	0.800476	1.415527e-48	6.597331e-46
MsG0480019024.01	MsG0580026102.01	0.813674	2.271684e-51	1.493045e-48
MsG0480019024.01	MsG0580026114.01	0.815343	9.704752e-52	6.673596e-49
MsG0480019024.01	MsG0580026311.01	0.812872	3.407314e-51	2.191183e-48
MsG0480019024.01	MsG0580026521.01	0.841158	5.630479e-58	8.200971e-55
MsG0480019024.01	MsG0580029359.01	0.807053	6.098881e-50	3.363149e-47
MsG0480019024.01	MsG0680031474.01	0.813831	2.096953e-51	1.384057e-48
MsG0480019024.01	MsG0680032932.01	0.805129	1.549381e-49	8.132409e-47
MsG0480019024.01	MsG0680033367.01	0.801340	9.431833e-49	4.493129e-46
MsG0480019024.01	MsG0680033368.01	0.843796	1.124006e-58	1.778425e-55
MsG0480019024.01	MsG0680033617.01	0.804272	2.339547e-49	1.201106e-46
MsG0480019024.01	MsG0680034238.01	0.814080	1.848127e-51	1.228088e-48
MsG0480019024.01	MsG0680034254.01	0.857710	1.355619e-62	3.376521e-59
MsG0480019024.01	MsG0680034310.01	0.808207	3.469434e-50	1.971812e-47
MsG0480019024.01	MsG0680034312.01	0.819290	1.255383e-52	9.620118e-50
MsG0480019024.01	MsG0680034332.01	0.812491	4.127942e-51	2.628393e-48
MsG0480019024.01	MsG0680034348.01	0.812837	3.467028e-51	2.227554e-48
MsG0480019024.01	MsG0680034354.01	0.842312	2.791821e-58	4.216500e-55
MsG0480019024.01	MsG0680034356.01	0.843339	1.489485e-58	2.323425e-55
MsG0480019024.01	MsG0680034357.01	0.809279	2.046385e-50	1.196444e-47
MsG0480019024.01	MsG0680034370.01	0.805467	1.316052e-49	6.967977e-47
MsG0480019024.01	MsG0680034418.01	0.851646	7.749773e-61	1.579043e-57
MsG0480019024.01	MsG0680034728.01	0.806536	7.841589e-50	4.266948e-47
MsG0480019024.01	MsG0680034974.01	0.809703	1.659694e-50	9.813579e-48
MsG0480019024.01	MsG0680034978.01	0.807260	5.512189e-50	3.056122e-47
MsG0480019024.01	MsG0780035930.01	0.807872	4.087204e-50	2.302312e-47
MsG0480019024.01	MsG0780036376.01	0.812686	3.741245e-51	2.394439e-48
MsG0480019024.01	MsG0780036472.01	0.805981	1.026775e-49	5.508681e-47
MsG0480019024.01	MsG0780036839.01	0.810108	1.358222e-50	8.116746e-48
MsG0480019024.01	MsG0780038313.01	0.801610	8.303258e-49	3.982404e-46
MsG0480019024.01	MsG0780038519.01	0.804472	2.125223e-49	1.096700e-46
MsG0480019024.01	MsG0780038657.01	0.850970	1.202766e-60	2.397609e-57
MsG0480019024.01	MsG0780039855.01	0.805037	1.619249e-49	8.478910e-47
MsG0480019024.01	MsG0780040497.01	0.836172	1.093506e-56	1.367225e-53
MsG0480019024.01	MsG0780040498.01	0.809493	1.841866e-50	1.082964e-47
MsG0480019024.01	MsG0780041036.01	0.801791	7.620934e-49	3.672104e-46
MsG0480019024.01	MsG0880042369.01	0.820283	7.442222e-53	5.863460e-50
MsG0480019024.01	MsG0880042633.01	0.806055	9.905990e-50	5.324517e-47
MsG0480019024.01	MsG0880042640.01	0.814691	1.353990e-51	9.147440e-49
MsG0480019024.01	MsG0880045801.01	0.804001	2.663364e-49	1.357955e-46
MsG0480019024.01	MsG0880045851.01	0.824126	9.557195e-54	8.390307e-51
MsG0480019024.01	MsG0880045854.01	0.823422	1.397273e-53	1.202473e-50
MsG0480019024.01	MsG0880046037.01	0.814635	1.393834e-51	9.402430e-49
MsG0480019024.01	MsG0880046071.01	0.811019	8.631760e-51	5.284784e-48
MsG0480019024.01	MsG0880046273.01	0.826639	2.429643e-54	2.291649e-51
MsG0480019024.01	MsG0880046946.01	0.801147	1.032902e-48	4.896770e-46
MsG0380016299.01	MsG0480019024.01	0.813528	2.445210e-51	1.600816e-48
MsG0380017901.01	MsG0480019024.01	0.818929	1.516409e-52	1.150773e-49
MsG0380018004.01	MsG0480019024.01	0.802475	5.511664e-49	2.702430e-46
MsG0480018433.01	MsG0480019024.01	0.815180	1.054720e-51	7.220110e-49
MsG0480018438.01	MsG0480019024.01	0.814139	1.794161e-51	1.194150e-48
MsG0280007909.01	MsG0480019024.01	0.808342	3.247173e-50	1.852053e-47
MsG0280007937.01	MsG0480019024.01	0.804271	2.340432e-49	1.201533e-46
MsG0280009232.01	MsG0480019024.01	0.854749	1.000504e-61	2.257460e-58
MsG0280009253.01	MsG0480019024.01	0.814508	1.486585e-51	9.994419e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019024.01.T01	MTR_4g022290	97.674	43	1	0	25	67	45	87	2.79e-23	94.4
MsG0480019024.01.T01	MTR_4g094828	95.349	43	2	0	25	67	44	86	3.08e-22	91.3
MsG0480019024.01.T01	MTR_8g079710	84.091	44	7	0	25	68	44	87	7.49e-18	79.0
MsG0480019024.01.T01	MTR_4g094835	66.667	42	12	1	25	64	47	88	6.18e-12	62.0
MsG0480019024.01.T01	MTR_4g094842	55.000	60	21	2	15	68	32	91	2.17e-11	60.5
MsG0480019024.01.T01	MTR_8g041770	53.061	49	19	1	21	65	41	89	8.19e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 30 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGTCTATTTGGTTTGTTT+TGG	0.227446	4:+13413538	None:intergenic
GGGATCTCAATTTCTCAAAT+TGG	0.253765	4:-13413593	MsG0480019024.01.T01:CDS
TTTGTTTAAGATGGTCTATT+TGG	0.294119	4:+13413528	None:intergenic
GATGGAACATGAGGCGTGTT+AGG	0.358459	4:+13413437	None:intergenic
TTAATCGACGGTATCTTCAT+TGG	0.408333	4:-13413241	MsG0480019024.01.T01:CDS
AGGAAAGTGTTTGTTTAAGA+TGG	0.419599	4:+13413519	None:intergenic
AAATTGTTCATTTATCTCCA+TGG	0.445544	4:-13413614	MsG0480019024.01.T01:CDS
TTATGGAAAAGAAGACCTCT+TGG	0.472134	4:+13413563	None:intergenic
AATTGTTCATTTATCTCCAT+GGG	0.481963	4:-13413613	MsG0480019024.01.T01:CDS
TATGGAAAAGAAGACCTCTT+GGG	0.486710	4:+13413564	None:intergenic
TAAGAATAATGAAGAAACAA+TGG	0.493631	4:+13413326	None:intergenic
ACAATGGGAGGAACATACTT+TGG	0.495798	4:+13413342	None:intergenic
AAGAATAATGAAGAAACAAT+GGG	0.496513	4:+13413327	None:intergenic
ACCAGCGTTGTTACAAATGA+TGG	0.505482	4:+13413419	None:intergenic
AACTTCTTTCCACCACTCGC+TGG	0.516081	4:-13413488	MsG0480019024.01.T01:CDS
TTTGAGAAATTGAGATCCCA+TGG	0.534424	4:+13413597	None:intergenic
TGGTTGAAGTATGTCGGCAA+TGG	0.566982	4:+13413362	None:intergenic
ATACTTTGGTTGAAGTATGT+CGG	0.572459	4:+13413356	None:intergenic
TGGTGGAAAGAAGTTAAGTG+TGG	0.592664	4:+13413496	None:intergenic
GTTACTTGTACTGCAAGTAG+TGG	0.602193	4:+13413268	None:intergenic
GAAAAGAAGACCTCTTGGGT+TGG	0.614988	4:+13413568	None:intergenic
TCCATCATTTGTAACAACGC+TGG	0.618527	4:-13413420	MsG0480019024.01.T01:CDS
ACAAGACGACCAGCGAGTGG+TGG	0.630223	4:+13413479	None:intergenic
GTTACAAATGATGGAACATG+AGG	0.636643	4:+13413428	None:intergenic
TGGAAAGAAGTTAAGTGTGG+AGG	0.665000	4:+13413499	None:intergenic
CACGATGTGAAAAGCTACGA+AGG	0.670201	4:-13413301	MsG0480019024.01.T01:CDS
CAAGTAACAGAGTTAATCGA+CGG	0.676652	4:-13413253	MsG0480019024.01.T01:CDS
AATAATGAAGAAACAATGGG+AGG	0.681867	4:+13413330	None:intergenic
CAAATTGGCACCAACCCAAG+AGG	0.684589	4:-13413578	MsG0480019024.01.T01:CDS
ATTACAAGACGACCAGCGAG+TGG	0.695572	4:+13413476	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATAATGAAGAAACAAT+GGG	+	Chr4:13413537-13413556	None:intergenic	20.0%
!!	TAAGAATAATGAAGAAACAA+TGG	+	Chr4:13413538-13413557	None:intergenic	20.0%
!	AAATTGTTCATTTATCTCCA+TGG	-	Chr4:13413247-13413266	MsG0480019024.01.T01:CDS	25.0%
!	AATTGTTCATTTATCTCCAT+GGG	-	Chr4:13413248-13413267	MsG0480019024.01.T01:CDS	25.0%
!!	TTTGTTTAAGATGGTCTATT+TGG	+	Chr4:13413336-13413355	None:intergenic	25.0%
	AATAATGAAGAAACAATGGG+AGG	+	Chr4:13413534-13413553	None:intergenic	30.0%
	ATACTTTGGTTGAAGTATGT+CGG	+	Chr4:13413508-13413527	None:intergenic	30.0%
!!	AGGAAAGTGTTTGTTTAAGA+TGG	+	Chr4:13413345-13413364	None:intergenic	30.0%
!!!	ATGGTCTATTTGGTTTGTTT+TGG	+	Chr4:13413326-13413345	None:intergenic	30.0%
!!!	TGGTCTATTTGGTTTGTTTT+GGG	+	Chr4:13413325-13413344	None:intergenic	30.0%
!!!	TTTGGTTTGTTTTGGGTTTA+TGG	+	Chr4:13413318-13413337	None:intergenic	30.0%
	CAAGTAACAGAGTTAATCGA+CGG	-	Chr4:13413608-13413627	MsG0480019024.01.T01:CDS	35.0%
	GGGATCTCAATTTCTCAAAT+TGG	-	Chr4:13413268-13413287	MsG0480019024.01.T01:CDS	35.0%
	TATGGAAAAGAAGACCTCTT+GGG	+	Chr4:13413300-13413319	None:intergenic	35.0%
	TTAATCGACGGTATCTTCAT+TGG	-	Chr4:13413620-13413639	MsG0480019024.01.T01:CDS	35.0%
	TTATGGAAAAGAAGACCTCT+TGG	+	Chr4:13413301-13413320	None:intergenic	35.0%
	TTTGAGAAATTGAGATCCCA+TGG	+	Chr4:13413267-13413286	None:intergenic	35.0%
!	GTTACAAATGATGGAACATG+AGG	+	Chr4:13413436-13413455	None:intergenic	35.0%
	ACAATGGGAGGAACATACTT+TGG	+	Chr4:13413522-13413541	None:intergenic	40.0%
	ACCAGCGTTGTTACAAATGA+TGG	+	Chr4:13413445-13413464	None:intergenic	40.0%
	GTTACTTGTACTGCAAGTAG+TGG	+	Chr4:13413596-13413615	None:intergenic	40.0%
	TCCATCATTTGTAACAACGC+TGG	-	Chr4:13413441-13413460	MsG0480019024.01.T01:CDS	40.0%
	TGGAAAGAAGTTAAGTGTGG+AGG	+	Chr4:13413365-13413384	None:intergenic	40.0%
	TGGTGGAAAGAAGTTAAGTG+TGG	+	Chr4:13413368-13413387	None:intergenic	40.0%
!!	AATGGTAGTGTTTTCTGCTG+TGG	+	Chr4:13413484-13413503	None:intergenic	40.0%
	CACGATGTGAAAAGCTACGA+AGG	-	Chr4:13413560-13413579	MsG0480019024.01.T01:CDS	45.0%
	GAAAAGAAGACCTCTTGGGT+TGG	+	Chr4:13413296-13413315	None:intergenic	45.0%
	TGGTTGAAGTATGTCGGCAA+TGG	+	Chr4:13413502-13413521	None:intergenic	45.0%
	AACTTCTTTCCACCACTCGC+TGG	-	Chr4:13413373-13413392	MsG0480019024.01.T01:CDS	50.0%
	ATTACAAGACGACCAGCGAG+TGG	+	Chr4:13413388-13413407	None:intergenic	50.0%
	CAAATTGGCACCAACCCAAG+AGG	-	Chr4:13413283-13413302	MsG0480019024.01.T01:CDS	50.0%
	GATGGAACATGAGGCGTGTT+AGG	+	Chr4:13413427-13413446	None:intergenic	50.0%
!	TAGTGTTTTCTGCTGTGGCG+TGG	+	Chr4:13413479-13413498	None:intergenic	50.0%
	ACAAGACGACCAGCGAGTGG+TGG	+	Chr4:13413385-13413404	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	13413229	13413654	13413229	ID=MsG0480019024.01;Name=MsG0480019024.01
Chr4	mRNA	13413229	13413654	13413229	ID=MsG0480019024.01.T01;Parent=MsG0480019024.01;Name=MsG0480019024.01.T01;_AED=0.33;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|141
Chr4	exon	13413229	13413654	13413229	ID=MsG0480019024.01.T01:exon:8395;Parent=MsG0480019024.01.T01
Chr4	CDS	13413229	13413654	13413229	ID=MsG0480019024.01.T01:cds;Parent=MsG0480019024.01.T01

Gene Sequence

>MsG0480019024.01.T01

ATGGCAACTCAACTCACAAAATTGTTCATTTATCTCCATGGGATCTCAATTTCTCAAATTGGCACCAACCCAAGAGGTCTTCTTTTCCATAAACCCAAAACAAACCAAATAGACCATCTTAAACAAACACTTTCCTCCACACTTAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAACACGCCTCATGTTCCATCATTTGTAACAACGCTGGTGCATTGTTTGTCCACGCCACAGCAGAAAACACTACCATTGCCGACATACTTCAACCAAAGTATGTTCCTCCCATTGTTTCTTCATTATTCTTACTCACGATGTGAAAAGCTACGAAGGCACGTCACAACCACTACTTGCAGTACAAGTAACAGAGTTAATCGACGGTATCTTCATTGGTTTTGCAATTAA

Protein sequence

>MsG0480019024.01.T01

MATQLTKLFIYLHGISISQIGTNPRGLLFHKPKTNQIDHLKQTLSSTLNFFPPLAGRLVITQHDEPNTPHVPSFVTTLVHCLSTPQQKTLPLPTYFNQSMFLPLFLHYSYSRCEKLRRHVTTTTCSTSNRVNRRYLHWFCN*