AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034109.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034109.01.T01 MTR_4g061200 84.783 92 14 0 10 101 155 246 3.51e-42 143
MsG0680034109.01.T01 MTR_4g061200 84.783 92 14 0 10 101 155 246 5.48e-42 143
MsG0680034109.01.T01 MTR_4g061200 84.783 92 14 0 10 101 155 246 1.50e-41 140
MsG0680034109.01.T01 MTR_4g061200 84.783 92 14 0 10 101 155 246 2.89e-41 139
MsG0680034109.01.T01 MTR_3g090510 89.773 88 9 0 14 101 163 250 5.51e-41 139
MsG0680034109.01.T01 MTR_7g060560 84.091 88 13 1 14 101 156 242 5.22e-35 123
MsG0680034109.01.T01 MTR_7g100590 72.826 92 25 0 10 101 163 254 1.08e-34 124
MsG0680034109.01.T01 MTR_4g094868 72.826 92 25 0 10 101 44 135 1.93e-34 121
MsG0680034109.01.T01 MTR_5g016810 72.826 92 25 0 10 101 176 267 4.88e-34 122
MsG0680034109.01.T01 MTR_4g094868 72.826 92 25 0 10 101 200 291 8.18e-34 122
MsG0680034109.01.T01 MTR_4g094868 72.826 92 25 0 10 101 200 291 8.49e-34 122
MsG0680034109.01.T01 MTR_7g061190 75.000 88 22 0 14 101 9 96 1.23e-33 114
MsG0680034109.01.T01 MTR_5g016810 72.527 91 25 0 10 100 176 266 2.55e-33 120
MsG0680034109.01.T01 MTR_2g093060 71.739 92 26 0 10 101 158 249 2.90e-33 119
MsG0680034109.01.T01 MTR_2g093060 71.739 92 26 0 10 101 158 249 3.26e-33 119
MsG0680034109.01.T01 MTR_2g093060 71.739 92 26 0 10 101 158 249 3.29e-33 119
MsG0680034109.01.T01 MTR_1g037030 85.417 48 7 0 52 99 118 165 2.09e-23 89.7
MsG0680034109.01.T01 MTR_4g097520 45.556 90 48 1 13 101 347 436 3.65e-21 87.0
MsG0680034109.01.T01 MTR_3g103460 45.556 90 48 1 13 101 347 436 6.12e-21 86.3
MsG0680034109.01.T01 MTR_7g009410 45.556 90 48 1 13 101 102 191 2.95e-20 84.0
MsG0680034109.01.T01 MTR_8g020510 44.444 90 49 1 13 101 285 374 3.60e-20 84.0
MsG0680034109.01.T01 MTR_5g015070 43.011 93 52 1 10 101 286 378 6.77e-20 83.2
MsG0680034109.01.T01 MTR_4g127930 40.860 93 54 1 10 101 196 288 7.23e-20 83.2
MsG0680034109.01.T01 MTR_7g080460 39.785 93 55 1 10 101 296 388 2.22e-19 81.6
MsG0680034109.01.T01 MTR_1g017400 42.222 90 51 1 13 101 349 438 2.56e-19 81.6
MsG0680034109.01.T01 MTR_6g011490 44.444 90 49 1 13 101 98 187 3.52e-19 80.9
MsG0680034109.01.T01 MTR_4g130270 41.935 93 53 1 10 101 84 176 5.93e-19 80.1
MsG0680034109.01.T01 MTR_8g044070 45.556 90 48 1 13 101 92 181 1.07e-18 79.7
MsG0680034109.01.T01 MTR_5g031880 44.444 90 49 1 13 101 261 350 1.15e-18 79.7
MsG0680034109.01.T01 MTR_8g068510 45.556 90 48 1 13 101 168 257 2.38e-18 78.6
MsG0680034109.01.T01 MTR_4g065370 37.634 93 57 1 10 101 200 292 2.47e-18 79.0
MsG0680034109.01.T01 MTR_2g098180 38.710 93 56 1 10 101 204 296 4.70e-18 77.8
MsG0680034109.01.T01 MTR_7g055620 76.190 42 10 0 60 101 6 47 8.20e-16 71.2
MsG0680034109.01.T01 MTR_8g044040 44.444 90 49 1 13 101 93 182 3.76e-15 69.7
MsG0680034109.01.T01 MTR_7g111940 88.636 44 5 0 9 52 115 158 1.12e-14 66.2
MsG0680034109.01.T01 MTR_8g468920 45.556 90 48 1 13 101 106 195 2.35e-14 67.4
MsG0680034109.01.T01 MTR_4g007770 44.444 90 49 1 13 101 73 162 1.40e-12 62.0
MsG0680034109.01.T01 MTR_1g049140 53.488 86 38 2 10 94 161 245 2.66e-12 61.6
MsG0680034109.01.T01 MTR_1g049140 53.488 86 38 2 10 94 161 245 5.18e-12 60.5
MsG0680034109.01.T01 MTR_1g049140 51.064 94 43 3 10 101 161 253 9.45e-12 59.3
MsG0680034109.01.T01 MTR_7g091390 41.111 90 49 1 10 99 3 88 1.13e-11 59.7
MsG0680034109.01.T01 MTR_2g460730 41.096 73 42 1 30 101 101 173 2.47e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034109.01.T01 AT2G28550 71.717 99 28 0 3 101 4 102 4.38e-36 124
MsG0680034109.01.T01 AT4G36920 75.000 92 23 0 10 101 155 246 1.23e-35 125
MsG0680034109.01.T01 AT4G36920 75.000 92 23 0 10 101 155 246 1.23e-35 125
MsG0680034109.01.T01 AT2G28550 73.913 92 24 0 10 101 177 268 2.18e-34 119
MsG0680034109.01.T01 AT2G28550 73.913 92 24 0 10 101 177 268 2.18e-34 119
MsG0680034109.01.T01 AT2G28550 73.913 92 24 0 10 101 177 268 6.96e-34 119
MsG0680034109.01.T01 AT2G28550 73.913 92 24 0 10 101 177 268 1.06e-33 120
MsG0680034109.01.T01 AT2G28550 73.913 92 24 0 10 101 177 268 1.53e-33 120
MsG0680034109.01.T01 AT5G67180 69.565 92 28 0 10 101 120 211 6.11e-32 114
MsG0680034109.01.T01 AT5G67180 69.565 92 28 0 10 101 120 211 1.65e-31 111
MsG0680034109.01.T01 AT5G67180 69.565 92 28 0 10 101 120 211 3.13e-31 111
MsG0680034109.01.T01 AT5G67180 69.565 92 28 0 10 101 120 211 3.40e-31 111
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 1.80e-22 90.5
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 1.83e-22 90.5
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 2.02e-22 90.5
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 1.07e-21 87.8
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 1.07e-21 87.8
MsG0680034109.01.T01 AT5G60120 58.889 90 37 0 10 99 184 273 1.07e-21 87.8
MsG0680034109.01.T01 AT2G39250 54.930 71 32 0 10 80 132 202 1.17e-21 87.0
MsG0680034109.01.T01 AT2G39250 52.000 75 36 0 10 84 132 206 4.52e-21 84.0
MsG0680034109.01.T01 AT5G57390 40.860 93 54 1 10 101 119 211 3.73e-20 84.0
MsG0680034109.01.T01 AT5G57390 40.860 93 54 1 10 101 237 329 4.38e-20 84.0
MsG0680034109.01.T01 AT1G72570 40.860 93 54 1 10 101 257 349 5.04e-20 83.2
MsG0680034109.01.T01 AT1G51190 39.785 93 55 1 10 101 224 316 1.08e-19 82.8
MsG0680034109.01.T01 AT5G17430 40.860 93 54 1 10 101 244 336 2.16e-19 82.0
MsG0680034109.01.T01 AT5G17430 40.860 93 54 1 10 101 182 274 2.32e-19 81.6
MsG0680034109.01.T01 AT5G65510 41.935 93 53 1 10 101 265 357 3.27e-19 81.3
MsG0680034109.01.T01 AT5G65510 41.935 93 53 1 10 101 265 357 3.27e-19 81.3
MsG0680034109.01.T01 AT5G65510 41.935 93 53 1 10 101 207 299 4.60e-19 80.9
MsG0680034109.01.T01 AT4G37750 40.860 93 54 1 10 101 317 409 5.35e-19 80.9
MsG0680034109.01.T01 AT3G54320 40.860 93 54 1 10 101 33 125 6.67e-19 80.1
MsG0680034109.01.T01 AT3G54320 40.860 93 54 1 10 101 33 125 6.67e-19 80.1
MsG0680034109.01.T01 AT5G10510 40.860 93 54 1 10 101 302 394 6.87e-19 80.5
MsG0680034109.01.T01 AT5G10510 40.860 93 54 1 10 101 302 394 6.87e-19 80.5
MsG0680034109.01.T01 AT3G54320 40.860 93 54 1 10 101 99 191 7.36e-19 80.1
MsG0680034109.01.T01 AT5G10510 40.860 93 54 1 10 101 264 356 8.85e-19 80.1
MsG0680034109.01.T01 AT5G10510 40.860 93 54 1 10 101 264 356 8.85e-19 80.1
MsG0680034109.01.T01 AT3G54320 40.860 93 54 1 10 101 99 191 8.96e-19 80.1
MsG0680034109.01.T01 AT5G10510 40.860 93 54 1 10 101 287 379 9.17e-19 80.1
MsG0680034109.01.T01 AT5G65510 41.935 93 53 1 10 101 207 299 1.15e-18 79.0
MsG0680034109.01.T01 AT3G20840 37.634 93 57 1 10 101 215 307 3.29e-18 78.6
MsG0680034109.01.T01 AT5G10510 42.222 90 51 1 13 101 264 353 6.90e-18 77.4
MsG0680034109.01.T01 AT5G10510 40.594 101 58 2 2 101 315 414 2.40e-17 75.9
MsG0680034109.01.T01 AT3G54990 55.422 83 37 0 10 92 146 228 1.66e-16 72.0
MsG0680034109.01.T01 AT3G54990 58.974 78 32 0 10 87 146 223 3.27e-16 72.4
MsG0680034109.01.T01 AT1G79700 45.161 93 50 1 10 101 86 178 3.91e-15 69.3
MsG0680034109.01.T01 AT1G79700 45.161 93 50 1 10 101 10 102 6.89e-15 67.8
MsG0680034109.01.T01 AT1G16060 44.086 93 51 1 10 101 92 184 2.61e-14 67.0
MsG0680034109.01.T01 AT1G79700 46.154 91 48 1 12 101 6 96 3.02e-14 65.9
MsG0680034109.01.T01 AT1G16060 44.086 93 51 1 10 101 22 114 1.08e-13 65.1
MsG0680034109.01.T01 AT1G79700 43.956 91 50 1 10 99 86 176 2.64e-13 63.9
MsG0680034109.01.T01 AT2G41710 41.111 90 49 1 10 99 104 189 1.45e-11 59.7
MsG0680034109.01.T01 AT2G41710 41.111 90 49 1 10 99 104 189 1.50e-11 59.3
MsG0680034109.01.T01 AT2G41710 41.111 90 49 1 10 99 139 224 1.82e-11 59.3

Find 14 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCGAGCTGCGATTAAATTC+AGG 0.379752 6:+80768969 MsG0680034109.01.T01:CDS
TTATGATGATGATCTTAAAC+AGG 0.440099 6:+80769027 MsG0680034109.01.T01:CDS
TTTAATCGCAGCTCGATCAT+AGG 0.441826 6:-80768963 None:intergenic
TGGCAGTTGGGAAGCTCGAA+TGG 0.485503 6:+80769235 MsG0680034109.01.T01:CDS
GCGATTAAATTCAGGGGAGT+TGG 0.497882 6:+80768977 MsG0680034109.01.T01:CDS
CACTGCACAAATGTGGCAGT+TGG 0.508097 6:+80769222 MsG0680034109.01.T01:CDS
GCTCGAATGGGTCAATTCCT+TGG 0.510521 6:+80769248 MsG0680034109.01.T01:CDS
ACTGCACAAATGTGGCAGTT+GGG 0.511914 6:+80769223 MsG0680034109.01.T01:CDS
TCGAGCTGCGATTAAATTCA+GGG 0.538997 6:+80768970 MsG0680034109.01.T01:CDS
ACACAGCTCATGTTGCTGCT+AGG 0.553358 6:+80768858 MsG0680034109.01.T01:CDS
GGCAGTTGGGAAGCTCGAAT+GGG 0.571868 6:+80769236 MsG0680034109.01.T01:CDS
GGAGTAACACTGCACAAATG+TGG 0.639362 6:+80769215 MsG0680034109.01.T01:CDS
AGAGGGAGTTCGAAGTACAG+AGG 0.669918 6:+80769194 MsG0680034109.01.T01:CDS
CGAGCTGCGATTAAATTCAG+GGG 0.681626 6:+80768971 MsG0680034109.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATGATTTACTATTTT+AGG + Chr6:80768794-80768813 MsG0680034109.01.T01:intron 10.0%
!!! TTGTGTAATTAATTTTTTTA+TGG + Chr6:80768909-80768928 MsG0680034109.01.T01:intron 10.0%
!!! ACTATTTTTTTCTTTGATTT+TGG + Chr6:80768758-80768777 MsG0680034109.01.T01:intron 15.0%
!! AAATAAAAACTTGGAAAGAT+AGG - Chr6:80768526-80768545 None:intergenic 20.0%
!! TGAAGTGAAAAATAAAAACT+TGG - Chr6:80768535-80768554 None:intergenic 20.0%
!!! GTTTTGTATTTGATTTTTGT+AGG + Chr6:80768827-80768846 MsG0680034109.01.T01:CDS 20.0%
!! AAATCAATGTTTTCTCAAGA+GGG + Chr6:80769177-80769196 MsG0680034109.01.T01:CDS 25.0%
!! TTATGATGATGATCTTAAAC+AGG + Chr6:80769027-80769046 MsG0680034109.01.T01:CDS 25.0%
!!! TTGTATTTGATTTTTGTAGG+TGG + Chr6:80768830-80768849 MsG0680034109.01.T01:CDS 25.0%
CAGCAATAGAGAAAATCAAA+AGG - Chr6:80768640-80768659 None:intergenic 30.0%
TTGATGAATGTGAAACTAGA+TGG + Chr6:80769071-80769090 MsG0680034109.01.T01:intron 30.0%
! AGGCTCTGATATTCAAAATT+TGG - Chr6:80768946-80768965 None:intergenic 30.0%
! CAAATCAATGTTTTCTCAAG+AGG + Chr6:80769176-80769195 MsG0680034109.01.T01:CDS 30.0%
AGATCATTAGGATCATCTCA+GGG + Chr6:80768715-80768734 MsG0680034109.01.T01:intron 35.0%
AGATGATCCTAATGATCTGT+AGG - Chr6:80768713-80768732 None:intergenic 35.0%
CAAAAGTCCTACAGATCATT+AGG + Chr6:80768703-80768722 MsG0680034109.01.T01:intron 35.0%
! GTTTCAATTTGGGATGTTAC+TGG + Chr6:80768488-80768507 MsG0680034109.01.T01:intron 35.0%
ATCGAGCTGCGATTAAATTC+AGG + Chr6:80768969-80768988 MsG0680034109.01.T01:CDS 40.0%
CAGATCATTAGGATCATCTC+AGG + Chr6:80768714-80768733 MsG0680034109.01.T01:intron 40.0%
GATCATTAGGATCATCTCAG+GGG + Chr6:80768716-80768735 MsG0680034109.01.T01:intron 40.0%
TCGAGCTGCGATTAAATTCA+GGG + Chr6:80768970-80768989 MsG0680034109.01.T01:CDS 40.0%
TTTAATCGCAGCTCGATCAT+AGG - Chr6:80768966-80768985 None:intergenic 40.0%
ACTGCACAAATGTGGCAGTT+GGG + Chr6:80769223-80769242 MsG0680034109.01.T01:CDS 45.0%
CGAGCTGCGATTAAATTCAG+GGG + Chr6:80768971-80768990 MsG0680034109.01.T01:CDS 45.0%
GCGATTAAATTCAGGGGAGT+TGG + Chr6:80768977-80768996 MsG0680034109.01.T01:CDS 45.0%
GGAGTAACACTGCACAAATG+TGG + Chr6:80769215-80769234 MsG0680034109.01.T01:CDS 45.0%
ACACAGCTCATGTTGCTGCT+AGG + Chr6:80768858-80768877 MsG0680034109.01.T01:CDS 50.0%
AGAGGGAGTTCGAAGTACAG+AGG + Chr6:80769194-80769213 MsG0680034109.01.T01:CDS 50.0%
CACTGCACAAATGTGGCAGT+TGG + Chr6:80769222-80769241 MsG0680034109.01.T01:CDS 50.0%
CATTAGGATCATCTCAGGGG+TGG + Chr6:80768719-80768738 MsG0680034109.01.T01:intron 50.0%
GCTCGAATGGGTCAATTCCT+TGG + Chr6:80769248-80769267 MsG0680034109.01.T01:CDS 50.0%
AGGATCATCTCAGGGGTGGA+AGG + Chr6:80768723-80768742 MsG0680034109.01.T01:intron 55.0%
GGCAGTTGGGAAGCTCGAAT+GGG + Chr6:80769236-80769255 MsG0680034109.01.T01:CDS 55.0%
TGGCAGTTGGGAAGCTCGAA+TGG + Chr6:80769235-80769254 MsG0680034109.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 80768480 80769280 80768480 ID=MsG0680034109.01;Name=MsG0680034109.01
Chr6 mRNA 80768480 80769280 80768480 ID=MsG0680034109.01.T01;Parent=MsG0680034109.01;Name=MsG0680034109.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0.75|0.6|1|0.5|0.6|5|0|101
Chr6 exon 80769131 80769280 80769131 ID=MsG0680034109.01.T01:exon:8313;Parent=MsG0680034109.01.T01
Chr6 exon 80768961 80769048 80768961 ID=MsG0680034109.01.T01:exon:8312;Parent=MsG0680034109.01.T01
Chr6 exon 80768816 80768879 80768816 ID=MsG0680034109.01.T01:exon:8311;Parent=MsG0680034109.01.T01
Chr6 exon 80768480 80768483 80768480 ID=MsG0680034109.01.T01:exon:8310;Parent=MsG0680034109.01.T01
Chr6 CDS 80768480 80768483 80768480 ID=MsG0680034109.01.T01:cds;Parent=MsG0680034109.01.T01
Chr6 CDS 80768816 80768879 80768816 ID=MsG0680034109.01.T01:cds;Parent=MsG0680034109.01.T01
Chr6 CDS 80768961 80769048 80768961 ID=MsG0680034109.01.T01:cds;Parent=MsG0680034109.01.T01
Chr6 CDS 80769131 80769280 80769131 ID=MsG0680034109.01.T01:cds;Parent=MsG0680034109.01.T01
Gene Sequence

>MsG0680034109.01.T01

ATGGGCATTGTTTTAGTTTTGTATTTGATTTTTGTAGGTGGATTTAACACAGCTCATGTTGCTGCTAGAGCCTATGATCGAGCTGCGATTAAATTCAGGGGAGTTGGTGCTTACATAAATTTCAATTTGAATGATTATGATGATGATCTTAAACAGACGAAGAATTTATCGAAAGATGAGTCTTTGCAAACACTTCGTCTACAAATCAATGTTTTCTCAAGAGGGAGTTCGAAGTACAGAGGAGTAACACTGCACAAATGTGGCAGTTGGGAAGCTCGAATGGGTCAATTCCTTGGTAAAAAGTGA

Protein sequence

>MsG0680034109.01.T01

MGIVLVLYLIFVGGFNTAHVAARAYDRAAIKFRGVGAYINFNLNDYDDDLKQTKNLSKDESLQTLRLQINVFSRGSSKYRGVTLHKCGSWEARMGQFLGKK*