AlfalfaGEDB | MsG0280010547.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010547.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010547.01.T01	RHN75408.1	54	100	14	5	226	317	86	161	1.42E-13	79.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010547.01.T01	A0A396JGD3	54.000	100	14	5	226	317	86	161	6.78e-14	79.3

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048383.01	MsG0280010547.01	0.817504	3.186048e-52	2.324521e-49
MsG0180000023.01	MsG0280010547.01	0.804244	2.371036e-49	1.216349e-46
MsG0180000611.01	MsG0280010547.01	0.800009	1.762305e-48	8.116748e-46
MsG0180003822.01	MsG0280010547.01	0.829234	5.770557e-55	5.871115e-52
MsG0180004016.01	MsG0280010547.01	0.807515	4.868836e-50	2.717339e-47
MsG0180004160.01	MsG0280010547.01	0.802311	5.958032e-49	2.909318e-46
MsG0180004486.01	MsG0280010547.01	0.803187	3.929809e-49	1.962125e-46
MsG0180004991.01	MsG0280010547.01	0.800785	1.224724e-48	5.752790e-46
MsG0180005049.01	MsG0280010547.01	0.802429	5.635292e-49	2.759897e-46
MsG0180005443.01	MsG0280010547.01	0.804652	1.949312e-49	1.010630e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010547.01.T01	MTR_2g084815	54.000	100	14	5	226	317	188	263	3.43e-17	80.9
MsG0280010547.01.T01	MTR_2g084815	54.000	100	14	5	226	317	211	286	5.05e-17	80.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 71 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCGATCCTGAGAAAAC+CGG	0.531933	2:-71283124	MsG0280010547.01.T01:intron
AAATGATGGATCTTGGAGGC+TGG	0.479023	2:-71284026	MsG0280010547.01.T01:CDS
AACACAATAACTTAAGAACT+TGG	0.506339	2:+71282987	None:intergenic
AAGTCTGGAGGACAGGACAG+TGG	0.693625	2:-71282256	MsG0280010547.01.T01:CDS
AATGAAAACAGCCTTGTCAT+TGG	0.425093	2:+71283960	None:intergenic
AATGATGGATCTTGGAGGCT+GGG	0.575002	2:-71284025	MsG0280010547.01.T01:CDS
ACAACCTTCACTTCTTCTGC+AGG	0.504350	2:+71283341	None:intergenic
ACGAGCTTTATCAGAAGATT+AGG	0.413583	2:-71283903	MsG0280010547.01.T01:intron
ACGTAAATAGCCGAATTCTT+TGG	0.277920	2:-71284767	MsG0280010547.01.T01:CDS
ACGTGCAAGTGCAGCCTCCA+TGG	0.541146	2:+71283379	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCGTAAAATTACGAGAGAA+TGG	+	Chr2:71282706-71282725	None:intergenic	30.0%
CTTAAACAAGATGACATTAC+TGG	-	Chr2:71282273-71282292	MsG0280010547.01.T01:CDS	30.0%
TAACTTAAGAACTTGGCAAA+TGG	+	Chr2:71284011-71284030	None:intergenic	30.0%
TAGCGAATAGTATAGTGAAT+TGG	+	Chr2:71282669-71282688	None:intergenic	30.0%
TCATTTCATAGAACACAACA+AGG	-	Chr2:71282572-71282591	MsG0280010547.01.T01:intron	30.0%
TCGTTTGATAAATCTTCTTG+AGG	-	Chr2:71282821-71282840	MsG0280010547.01.T01:intron	30.0%
TTGTAAATAAACTTTCCCCT+CGG	-	Chr2:71283314-71283333	MsG0280010547.01.T01:CDS	30.0%
AAACTAGGGAGGTTTATTAC+AGG	+	Chr2:71283484-71283503	None:intergenic	35.0%
AATGTTTCAATCTAAGGGTG+AGG	+	Chr2:71284522-71284541	None:intergenic	35.0%
ACGTAAATAGCCGAATTCTT+TGG	-	Chr2:71282235-71282254	MsG0280010547.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	71282192	71284832	71282192	ID=MsG0280010547.01;Name=MsG0280010547.01
Chr2	mRNA	71282192	71284832	71282192	ID=MsG0280010547.01.T01;Parent=MsG0280010547.01;Name=MsG0280010547.01.T01;_AED=0.40;_eAED=0.50;_QI=0\|0.33\|0.42\|0.85\|1\|1\|7\|0\|330
Chr2	exon	71284729	71284832	71284729	ID=MsG0280010547.01.T01:exon:6791;Parent=MsG0280010547.01.T01
Chr2	exon	71284603	71284631	71284603	ID=MsG0280010547.01.T01:exon:6790;Parent=MsG0280010547.01.T01
Chr2	exon	71283904	71284237	71283904	ID=MsG0280010547.01.T01:exon:6789;Parent=MsG0280010547.01.T01
Chr2	exon	71283292	71283508	71283292	ID=MsG0280010547.01.T01:exon:6788;Parent=MsG0280010547.01.T01
Chr2	exon	71283125	71283206	71283125	ID=MsG0280010547.01.T01:exon:6787;Parent=MsG0280010547.01.T01
Chr2	exon	71282956	71283024	71282956	ID=MsG0280010547.01.T01:exon:6786;Parent=MsG0280010547.01.T01
Chr2	exon	71282192	71282349	71282192	ID=MsG0280010547.01.T01:exon:6785;Parent=MsG0280010547.01.T01
Chr2	CDS	71284729	71284832	71284729	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71284603	71284631	71284603	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71283904	71284237	71283904	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71283292	71283508	71283292	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71283125	71283206	71283125	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71282956	71283024	71282956	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01
Chr2	CDS	71282192	71282349	71282192	ID=MsG0280010547.01.T01:cds;Parent=MsG0280010547.01.T01

Gene Sequence

>MsG0280010547.01.T01

ATGCGTAGGGGCATTTTGGTCTTTTACTTTGGGAATGAGCAAAACGTAAATAGCCGAATTCTTTGGGTAAAGAGCAGAATTCTTAAACAAGATGACATTACTGGCGGAAGCCATGGCATCTTCAAGTGGAGCATGAATACTATCGAGGAAGTGAACACCACTGAGGATCGTTTGATAAATCTTCTTGAGGCTCAAGCAATTTCCATTTTTCAGACTGGACTCGAAGAGGACAAGGAACATCCTTATCCTGAACGTGATTTTTCAAAGTTGACTGTTAGGACCCAACAGCTGTACATTGACGAAGTAACGAGGATTGTTCTTGAAATGATGGATCTTGGAGGCTGGGATGCTATGGCGCAAAAATATGAAAAGTACAGATTGTCTGAAGCCAATGACAAGGCTGTTTTCATTGAAATTTTAGCACTGACGGGAGCTTTGGAAGGCGACGAGCTTTATCAGAAGATTAGGCTTGAGGCTGATCGTGACCTGAAGGCTAAACATGCCATGGAGGCTGAACGTGCACGTGACATGGAGGCTATACGTGCACGTGGACGTGAGCTGGAGGCTAAACGTGCCATGGAGGCTGCACTTGCACGTGCCATGAATGCTGCACCTGCAGAAGAAGTGAAGGTTGTTGATCCAAACGAGGAGAAAGATGAAGATTCTGATGAGACTGTGAAGAAGGTTGACAAGGGAAAGAAGAGGCCTAATGAATCTGCTGCAGAAACTCCAGTTTCTTCCAAGAAAACCGATCCTGAGAAAACCGTGTTAAATCCATTTGCCAAGTTCTTAAGTTATTGTGTTGTTGATAGATCATGGATTGATGTTTGTGTTTATGGTAAGAAAGCTGGACATACAGCAATTCCTCACCCTAAGGAAGGTGGAAAGACTGATGCCAAATCTCCCAAGTCTGGAGGACAGGACAGTGGTTGCAGCAAGTATGCTTTTAACAAAACTCGATGCATATGTTTATTACTTTTAGATGGGTTGTGA

Protein sequence

>MsG0280010547.01.T01

MRRGILVFYFGNEQNVNSRILWVKSRILKQDDITGGSHGIFKWSMNTIEEVNTTEDRLINLLEAQAISIFQTGLEEDKEHPYPERDFSKLTVRTQQLYIDEVTRIVLEMMDLGGWDAMAQKYEKYRLSEANDKAVFIEILALTGALEGDELYQKIRLEADRDLKAKHAMEAERARDMEAIRARGRELEAKRAMEAALARAMNAAPAEEVKVVDPNEEKDEDSDETVKKVDKGKKRPNESAAETPVSSKKTDPEKTVLNPFAKFLSYCVVDRSWIDVCVYGKKAGHTAIPHPKEGGKTDAKSPKSGGQDSGCSKYAFNKTRCICLLLLDGL*