Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040190.01.T01 | XP_003624658.1 | 83.828 | 303 | 3 | 1 | 201 | 503 | 3 | 259 | 1.01E-171 | 494 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048383.01 | MsG0780040190.01 | 0.805730 | 1.159222e-49 | 6.179548e-47 |
| MsG0080048968.01 | MsG0780040190.01 | 0.800479 | 1.413520e-48 | 6.588462e-46 |
| MsG0080049135.01 | MsG0780040190.01 | 0.821084 | 4.872469e-53 | 3.925429e-50 |
| MsG0180000197.01 | MsG0780040190.01 | 0.802568 | 5.275509e-49 | 2.592667e-46 |
| MsG0180000604.01 | MsG0780040190.01 | 0.802196 | 6.293319e-49 | 3.063685e-46 |
| MsG0180000611.01 | MsG0780040190.01 | 0.819014 | 1.450907e-52 | 1.103569e-49 |
| MsG0180001311.01 | MsG0780040190.01 | 0.819350 | 1.216173e-52 | 9.335042e-50 |
| MsG0180001599.01 | MsG0780040190.01 | 0.808780 | 2.617073e-50 | 1.509751e-47 |
| MsG0180001922.01 | MsG0780040190.01 | 0.800021 | 1.752235e-48 | 8.072835e-46 |
| MsG0180002075.01 | MsG0780040190.01 | 0.804610 | 1.989061e-49 | 1.030106e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040190.01.T01 | MTR_7g086080 | 83.828 | 303 | 3 | 1 | 201 | 503 | 3 | 259 | 1.22e-175 | 494 |
| MsG0780040190.01.T01 | MTR_7g086090 | 90.500 | 200 | 17 | 1 | 1 | 198 | 1 | 200 | 3.42e-108 | 322 |
| MsG0780040190.01.T01 | MTR_7g086100 | 79.803 | 203 | 38 | 2 | 1 | 200 | 1 | 203 | 8.99e-78 | 244 |
| MsG0780040190.01.T01 | MTR_7g086230 | 76.730 | 159 | 35 | 2 | 1 | 159 | 1 | 157 | 2.62e-71 | 225 |
| MsG0780040190.01.T01 | MTR_7g086280 | 75.472 | 159 | 37 | 2 | 1 | 159 | 1 | 157 | 5.55e-70 | 221 |
| MsG0780040190.01.T01 | MTR_8g066460 | 41.639 | 305 | 114 | 4 | 202 | 504 | 4 | 246 | 1.23e-61 | 203 |
| MsG0780040190.01.T01 | MTR_8g066460 | 40.836 | 311 | 120 | 4 | 196 | 504 | 14 | 262 | 2.37e-61 | 203 |
| MsG0780040190.01.T01 | MTR_1g032860 | 35.110 | 319 | 137 | 4 | 178 | 496 | 13 | 261 | 3.33e-50 | 174 |
| MsG0780040190.01.T01 | MTR_5g029640 | 39.057 | 297 | 117 | 4 | 202 | 496 | 4 | 238 | 4.13e-50 | 178 |
| MsG0780040190.01.T01 | MTR_5g029640 | 49.242 | 132 | 67 | 0 | 365 | 496 | 279 | 410 | 4.00e-32 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040190.01.T01 | AT5G32450 | 61.889 | 307 | 70 | 2 | 198 | 503 | 1 | 261 | 1.30e-124 | 365 |
| MsG0780040190.01.T01 | AT4G17720 | 39.216 | 306 | 120 | 3 | 203 | 504 | 5 | 248 | 4.95e-56 | 190 |
| MsG0780040190.01.T01 | AT5G16840 | 38.435 | 294 | 114 | 4 | 199 | 492 | 3 | 229 | 1.15e-53 | 182 |
| MsG0780040190.01.T01 | AT5G16840 | 38.435 | 294 | 113 | 4 | 199 | 492 | 3 | 228 | 2.41e-53 | 181 |
| MsG0780040190.01.T01 | AT5G16840 | 38.488 | 291 | 111 | 4 | 202 | 492 | 4 | 226 | 5.49e-53 | 180 |
| MsG0780040190.01.T01 | AT5G46870 | 36.634 | 303 | 120 | 3 | 203 | 496 | 5 | 244 | 6.59e-53 | 181 |
| MsG0780040190.01.T01 | AT5G46870 | 36.634 | 303 | 120 | 3 | 203 | 496 | 5 | 244 | 6.59e-53 | 181 |
| MsG0780040190.01.T01 | AT1G67950 | 31.944 | 288 | 133 | 3 | 194 | 481 | 21 | 245 | 6.15e-43 | 154 |
| MsG0780040190.01.T01 | AT1G67950 | 32.292 | 288 | 131 | 3 | 194 | 481 | 21 | 244 | 1.49e-42 | 153 |
| MsG0780040190.01.T01 | AT1G67950 | 32.292 | 288 | 131 | 3 | 194 | 481 | 22 | 245 | 1.61e-42 | 153 |
Find 129 sgRNAs with CRISPR-Local
Find 431 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGTTTGTTTGTGGAGCTG+TGG | 0.613900 | 7:-74146276 | MsG0780040190.01.T01:CDS |
| AACACGAAAGACTTTGTTAA+CGG | 0.415458 | 7:+74151300 | None:intergenic |
| AACGCAATTTCAAGCGCTCT+GGG | 0.507323 | 7:+74146330 | None:intergenic |
| AACGTGCAGCCACCAAAGTT+TGG | 0.386453 | 7:-74150589 | MsG0780040190.01.T01:CDS |
| AACTAGAGAGAAGTTTAATA+TGG | 0.366039 | 7:-74144205 | MsG0780040190.01.T01:CDS |
| AACTGGTGCATTTAGCAAGG+TGG | 0.655445 | 7:-74144256 | MsG0780040190.01.T01:CDS |
| AAGTAATTTATGTAGGAAGT+GGG | 0.492069 | 7:-74145336 | MsG0780040190.01.T01:intron |
| AAGTGAAGTCTGTGGATCAG+AGG | 0.597757 | 7:-74144890 | MsG0780040190.01.T01:CDS |
| AATGTACCATCTTCTCCCAT+AGG | 0.572536 | 7:+74150657 | None:intergenic |
| AATGTCACAAATGCAGTCTT+TGG | 0.286462 | 7:+74146360 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAATAATGGTGCTTGGA+TGG | + | Chr7:74147469-74147488 | None:intergenic | 30.0% | |
| AAAAGACACATAAAAATGGG+TGG | + | Chr7:74146365-74146384 | None:intergenic | 30.0% | |
| AACACGAAAGACTTTGTTAA+CGG | + | Chr7:74144285-74144304 | None:intergenic | 30.0% | |
| ACACACATGAAAAAGAATCA+AGG | + | Chr7:74146126-74146145 | None:intergenic | 30.0% | |
| ACGAATCATCGTCAAAATAT+AGG | + | Chr7:74145938-74145957 | None:intergenic | 30.0% | |
| ACTAGAAAAGAATGGTTCAA+TGG | + | Chr7:74147421-74147440 | None:intergenic | 30.0% | |
| AGCTTCATTGTCTATATATC+AGG | - | Chr7:74150083-74150102 | MsG0780040190.01.T01:intron | 30.0% | |
| AGTAATTTATGTAGGAAGTG+GGG | - | Chr7:74150247-74150266 | MsG0780040190.01.T01:intron | 30.0% | |
| ATAAGCTCAATCACAAAAAC+AGG | + | Chr7:74150423-74150442 | None:intergenic | 30.0% | |
| ATAGATTTAGAAATCTCACC+GGG | + | Chr7:74144473-74144492 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 74144152 | 74151452 | 74144152 | ID=MsG0780040190.01;Name=MsG0780040190.01 |
| Chr7 | mRNA | 74144152 | 74151452 | 74144152 | ID=MsG0780040190.01.T01;Parent=MsG0780040190.01;Name=MsG0780040190.01.T01;_AED=0.40;_eAED=0.43;_QI=0|0.57|0.37|1|1|1|8|0|512 |
| Chr7 | exon | 74151287 | 74151452 | 74151287 | ID=MsG0780040190.01.T01:exon:10185;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74150369 | 74150802 | 74150369 | ID=MsG0780040190.01.T01:exon:10184;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74146837 | 74146940 | 74146837 | ID=MsG0780040190.01.T01:exon:10183;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74146234 | 74146395 | 74146234 | ID=MsG0780040190.01.T01:exon:10182;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74145428 | 74145551 | 74145428 | ID=MsG0780040190.01.T01:exon:10181;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74145281 | 74145343 | 74145281 | ID=MsG0780040190.01.T01:exon:10180;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74144804 | 74145135 | 74144804 | ID=MsG0780040190.01.T01:exon:10179;Parent=MsG0780040190.01.T01 |
| Chr7 | exon | 74144152 | 74144305 | 74144152 | ID=MsG0780040190.01.T01:exon:10178;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74151287 | 74151452 | 74151287 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74150369 | 74150802 | 74150369 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74146837 | 74146940 | 74146837 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74146234 | 74146395 | 74146234 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74145428 | 74145551 | 74145428 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74145281 | 74145343 | 74145281 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74144804 | 74145135 | 74144804 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Chr7 | CDS | 74144152 | 74144305 | 74144152 | ID=MsG0780040190.01.T01:cds;Parent=MsG0780040190.01.T01 |
| Gene Sequence |
>MsG0780040190.01.T01 ATGGCTCTTTCTCGTGCTCTTTTTCTCTTTGCTTTCATTGCTACTATCTTCTCAACCATGGCTGTGGCAAAAGATTTTGTAGCTGGAGATGAACGTGGTTGGAAATTAGGGGTTGATTATCAATATTGGGCCGTTAACAAAGTCTTTCGTGTTGGGGACACACTCACATTCAACTATGTCGGTGGTAAGGACAATGTGGTGAGAGTGAATGGAAGTGATTTCCAGAGTTGCTCAATTCCTTGGAGAGCACCGGTGTTAACTAGTGGACATGATACAATTTTATTGACAACCTATGGGAGAAGATGGTACATTTCAGGTGCTGCTCGTCACTGCAATCTTGGCCAAAAGCTTTTCATAAACGTGCAGCCACCAAAGTTTGGTTGGTCTCCAGTACCCTCACCTTCAGCCTCCCCATCACCCTCACCTGTGCCGGCCCCTGAAGCAGCACCGCCTTCAAATGTGCCATGGGCTGCTAGCGTGCAGACATCAGACATAACTTGGTCTCCGGTACCCTCACCCTCACCGGCCCATGAAGCAGCACCACCTTCAAATGCACCATGGGCTGCTACCGTGCAGACATCGGAGATAACTTGGTCTCCGACAAGGACGGTTCAAGTGAAGCAACTTTCAGATCTAGCTACTGAGAGGGAGATTCATGAATTCTTCTCATTCTCTGGTGAAATTGAACACATTCAGATCGTTGGAGAATATGGAAAACCAAAGACTGCATTTGTGACATTTAAAGATCCCAGAGCGCTTGAAATTGCGTTATTGTTATCGGTAATGTCTCTTTTCTTTTCAAAAGTTTGTTTGTGGAGCTGTGGTAACTTATCTATTGTCAAGTTTGATTTTTGGAGTCGGTTTGGTATTATCGAGTCAATTGCTCGTGGGTCTGAAGCTTCATTGTCTATATATCAGGGAGCAACAATAGTAGATCAGATTGTGAGTATAAGTCCAGTCGAGAACTATGTTGCAAATCGTGAGACGCAGGAAGTGGGGGTGGTAGAATATGCTATAAATATTGCTCCTGAAGATGCCGCGTCGAATACTGAGGAGGAGAAGCCTGGTGGAAGAGTATATCTTAGTAAAGCACAAGATGTGGTTACAACTATGCTGGCAAAAGGATCTGCGATAAGACAAGACGCTGTGAACAAAGCTAAAGCATTTGATGAGAAGCATCAGTTGACTGCCAATGCATCTGCAAAGGTCAGTTCTTTTGACAAGAGAGTTGGACTGACAGAGAAATTGACAGTTGGACTCTCCGTGGTGAATGAGAAAGTGAAGTCTGTGGATCAGAGGCTACATGTTTCAGATAAAACAATGGCAGCAATATTTGCAGCCGAGAGGAAGTTGAATGATACTGGATCAGCCGTCAAAACAAGCAGATATGTGACTGCTGGAACAACATGGTTAACTGGTGCATTTAGCAAGGTGGCAAGGGCTGGAAACGTTGCTGGAACTAAAACTAGAGAGAAGTTTAATATGGCCGTTTCAAACTTGACATCAAAGGTTGGATATAAGCTTGCCATGTCAAACTAA |
| Protein sequence |
>MsG0780040190.01.T01 MALSRALFLFAFIATIFSTMAVAKDFVAGDERGWKLGVDYQYWAVNKVFRVGDTLTFNYVGGKDNVVRVNGSDFQSCSIPWRAPVLTSGHDTILLTTYGRRWYISGAARHCNLGQKLFINVQPPKFGWSPVPSPSASPSPSPVPAPEAAPPSNVPWAASVQTSDITWSPVPSPSPAHEAAPPSNAPWAATVQTSEITWSPTRTVQVKQLSDLATEREIHEFFSFSGEIEHIQIVGEYGKPKTAFVTFKDPRALEIALLLSVMSLFFSKVCLWSCGNLSIVKFDFWSRFGIIESIARGSEASLSIYQGATIVDQIVSISPVENYVANRETQEVGVVEYAINIAPEDAASNTEEEKPGGRVYLSKAQDVVTTMLAKGSAIRQDAVNKAKAFDEKHQLTANASAKVSSFDKRVGLTEKLTVGLSVVNEKVKSVDQRLHVSDKTMAAIFAAERKLNDTGSAVKTSRYVTAGTTWLTGAFSKVARAGNVAGTKTREKFNMAVSNLTSKVGYKLAMSN* |
