Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034122.01.T01 | XP_013452470.1 | 96.816 | 534 | 10 | 4 | 1 | 529 | 1 | 532 | 0 | 1051 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034122.01.T01 | A0A072UBT9 | 96.816 | 534 | 10 | 4 | 1 | 529 | 1 | 532 | 0.0 | 1051 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048274.01 | MsG0680034122.01 | 0.823133 | 1.631580e-53 | 1.392895e-50 |
| MsG0080048887.01 | MsG0680034122.01 | 0.805627 | 1.218476e-49 | 6.477824e-47 |
| MsG0080048896.01 | MsG0680034122.01 | 0.814384 | 1.583425e-51 | 1.060980e-48 |
| MsG0080048900.01 | MsG0680034122.01 | 0.838244 | 3.227046e-57 | 4.296262e-54 |
| MsG0080048992.01 | MsG0680034122.01 | 0.850043 | 2.191709e-60 | 4.238449e-57 |
| MsG0080049016.01 | MsG0680034122.01 | 0.827799 | 1.281342e-54 | 1.249959e-51 |
| MsG0080049018.01 | MsG0680034122.01 | 0.831777 | 1.376785e-55 | 1.509442e-52 |
| MsG0080049133.01 | MsG0680034122.01 | 0.842418 | 2.618172e-58 | 3.967644e-55 |
| MsG0180000322.01 | MsG0680034122.01 | 0.814307 | 1.646692e-51 | 1.101073e-48 |
| MsG0180000417.01 | MsG0680034122.01 | 0.803114 | 4.067506e-49 | 2.027034e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034122.01.T01 | MTR_6g463260 | 96.816 | 534 | 10 | 4 | 1 | 529 | 1 | 532 | 0.0 | 1051 |
| MsG0680034122.01.T01 | MTR_5g011400 | 53.722 | 497 | 224 | 4 | 31 | 524 | 1 | 494 | 0.0 | 555 |
| MsG0680034122.01.T01 | MTR_4g097540 | 45.561 | 428 | 218 | 6 | 116 | 529 | 16 | 442 | 2.23e-142 | 419 |
| MsG0680034122.01.T01 | MTR_6g091760 | 44.471 | 425 | 230 | 3 | 102 | 522 | 12 | 434 | 3.01e-141 | 415 |
| MsG0680034122.01.T01 | MTR_8g101750 | 44.340 | 424 | 224 | 4 | 100 | 519 | 8 | 423 | 3.47e-138 | 407 |
| MsG0680034122.01.T01 | MTR_3g103550 | 44.988 | 429 | 227 | 3 | 104 | 524 | 2 | 429 | 3.51e-137 | 406 |
| MsG0680034122.01.T01 | MTR_5g015020 | 44.153 | 419 | 221 | 6 | 116 | 522 | 16 | 433 | 2.03e-136 | 404 |
| MsG0680034122.01.T01 | MTR_8g098930 | 41.647 | 425 | 241 | 3 | 104 | 522 | 12 | 435 | 6.13e-131 | 390 |
| MsG0680034122.01.T01 | MTR_8g028600 | 41.299 | 431 | 249 | 2 | 95 | 522 | 2 | 431 | 4.36e-127 | 380 |
| MsG0680034122.01.T01 | MTR_7g079080 | 41.135 | 423 | 244 | 3 | 104 | 522 | 11 | 432 | 2.23e-123 | 370 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034122.01.T01 | AT5G51690 | 60.279 | 501 | 189 | 3 | 31 | 527 | 1 | 495 | 0.0 | 635 |
| MsG0680034122.01.T01 | AT1G62960 | 52.799 | 536 | 211 | 8 | 29 | 529 | 29 | 557 | 0.0 | 558 |
| MsG0680034122.01.T01 | AT5G51690 | 61.081 | 370 | 141 | 1 | 161 | 527 | 3 | 372 | 7.50e-174 | 495 |
| MsG0680034122.01.T01 | AT4G37770 | 46.838 | 427 | 219 | 3 | 104 | 523 | 4 | 429 | 5.34e-144 | 423 |
| MsG0680034122.01.T01 | AT2G22810 | 45.057 | 435 | 230 | 4 | 102 | 528 | 2 | 435 | 5.32e-141 | 415 |
| MsG0680034122.01.T01 | AT3G49700 | 46.065 | 432 | 225 | 3 | 103 | 527 | 3 | 433 | 2.22e-139 | 411 |
| MsG0680034122.01.T01 | AT5G65800 | 44.952 | 416 | 221 | 3 | 116 | 524 | 16 | 430 | 8.90e-136 | 402 |
| MsG0680034122.01.T01 | AT4G26200 | 43.681 | 451 | 234 | 6 | 81 | 523 | 4 | 442 | 2.73e-134 | 397 |
| MsG0680034122.01.T01 | AT4G08040 | 43.826 | 413 | 227 | 3 | 116 | 524 | 14 | 425 | 6.34e-133 | 394 |
| MsG0680034122.01.T01 | AT4G11280 | 42.619 | 420 | 236 | 3 | 104 | 519 | 15 | 433 | 4.89e-130 | 388 |
Find 131 sgRNAs with CRISPR-Local
Find 358 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAATACGAAACCATCC+TGG | 0.595378 | 6:-81111822 | None:intergenic |
| AAACGAGGAGTCAAAGTTCG+CGG | 0.587306 | 6:+81111189 | MsG0680034122.01.T01:CDS |
| AAACTCCTCGCTTCCGTACG+TGG | 0.582162 | 6:-81111337 | None:intergenic |
| AAATCCCTCTAGTTATTGAT+AGG | 0.392176 | 6:+81111865 | MsG0680034122.01.T01:CDS |
| AACGATAGTATCTCAATTGC+AGG | 0.378929 | 6:-81110742 | None:intergenic |
| AACTTGGAGGGATCAATGAT+GGG | 0.518854 | 6:+81108244 | MsG0680034122.01.T01:CDS |
| AAGAAGAAGGTGGTTGAGAT+TGG | 0.316071 | 6:-81106476 | None:intergenic |
| AAGAAGCGTAGGAGAAGAAG+GGG | 0.509079 | 6:-81106664 | None:intergenic |
| AAGGAGGAGAAGAAGAAGGG+AGG | 0.559626 | 6:-81106343 | None:intergenic |
| AAGTGTGCTAAGAGCAGCGC+CGG | 0.573828 | 6:+81111666 | MsG0680034122.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTGTGAGGGATCAAAA+TGG | + | Chr6:81107830-81107849 | MsG0680034122.01.T01:intron | 30.0% | |
| AAATCCCTCTAGTTATTGAT+AGG | + | Chr6:81111865-81111884 | MsG0680034122.01.T01:CDS | 30.0% | |
| AAATTGTGAGGGATCAAAAT+GGG | + | Chr6:81107831-81107850 | MsG0680034122.01.T01:intron | 30.0% | |
| AATAAGGAGAGTAGATAAGA+TGG | + | Chr6:81108754-81108773 | MsG0680034122.01.T01:intron | 30.0% | |
| ACAACTATGTATGGGAAATA+TGG | - | Chr6:81109640-81109659 | None:intergenic | 30.0% | |
| ACAAGTCATTCTTGAAAAGA+TGG | + | Chr6:81110155-81110174 | MsG0680034122.01.T01:intron | 30.0% | |
| ACACAAAACAAGTTAGGATT+AGG | + | Chr6:81110105-81110124 | MsG0680034122.01.T01:intron | 30.0% | |
| ACTTCTGGTATAACTGATTA+AGG | - | Chr6:81109557-81109576 | None:intergenic | 30.0% | |
| AGAAAGGAGAACTTGAATTA+TGG | + | Chr6:81111736-81111755 | MsG0680034122.01.T01:CDS | 30.0% | |
| AGAAAGTAGAAGAGTGATAA+AGG | - | Chr6:81106597-81106616 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 81106327 | 81111926 | 81106327 | ID=MsG0680034122.01;Name=MsG0680034122.01 |
| Chr6 | mRNA | 81106327 | 81111926 | 81106327 | ID=MsG0680034122.01.T01;Parent=MsG0680034122.01;Name=MsG0680034122.01.T01;_AED=0.49;_eAED=0.49;_QI=108|0.66|0.75|1|1|1|4|0|529 |
| Chr6 | exon | 81111080 | 81111926 | 81111080 | ID=MsG0680034122.01.T01:exon:9613;Parent=MsG0680034122.01.T01 |
| Chr6 | exon | 81110658 | 81110818 | 81110658 | ID=MsG0680034122.01.T01:exon:9612;Parent=MsG0680034122.01.T01 |
| Chr6 | exon | 81108208 | 81108342 | 81108208 | ID=MsG0680034122.01.T01:exon:9611;Parent=MsG0680034122.01.T01 |
| Chr6 | exon | 81106327 | 81106881 | 81106327 | ID=MsG0680034122.01.T01:exon:9610;Parent=MsG0680034122.01.T01 |
| Chr6 | five_prime_UTR | 81106327 | 81106434 | 81106327 | ID=MsG0680034122.01.T01:five_prime_utr;Parent=MsG0680034122.01.T01 |
| Chr6 | CDS | 81106435 | 81106881 | 81106435 | ID=MsG0680034122.01.T01:cds;Parent=MsG0680034122.01.T01 |
| Chr6 | CDS | 81108208 | 81108342 | 81108208 | ID=MsG0680034122.01.T01:cds;Parent=MsG0680034122.01.T01 |
| Chr6 | CDS | 81110658 | 81110818 | 81110658 | ID=MsG0680034122.01.T01:cds;Parent=MsG0680034122.01.T01 |
| Chr6 | CDS | 81111080 | 81111926 | 81111080 | ID=MsG0680034122.01.T01:cds;Parent=MsG0680034122.01.T01 |
| Gene Sequence |
>MsG0680034122.01.T01 ATGACCCAAACCCAAAAAAAACCCACCAACCCCAAAAAAACCCAATCTCAACCACCTTCTTCTTCTTCATCCTCTCCCTCTCGAACCGGAATGAAACTCATCGTTCCTCTTCAAGCCGTAGTTCAAGGTCGCAACGGTTTTCTCCTCGGTAGTTTAATCCCTTTATCACTCTTCTACTTTCTTCAACTCTACCTTAAACGACGTCGTCCTGAGAATAATAATAACAATACCCCTTCTTCTCCTACGCTTCTTCCTCGTTCTTCTTCTCGTTCGAATTTATCCTCTCGTGGCTCTATTTCGAGTGTGAAAGTGTCGAAACTTGCGAATGTTGTTGCGAAACGTGATGATTCTCTTTATTATGTTGGAATTGAAAGAGTTGAGAAAGATCCTTATCATGTTAAGTTTAATCCTCATGGGATTATTCAGCTTGGTTTGTCTGATAACAAGTTGAGTTTGGATTTGATTGAGAAATGGTTGGTGAAGAACTTGGAGGGATCAATGATGGGGAATGAAGATATCAATTTGAGTATAAATGGAATTGCGCCTTATCAATCATTTCATGGATTGAATGAATTGAAAATTGCGTTGGCAGATTTTATGCATCAGGTTATGGGAGGATCAGTAGAATTTGACCCGTCAAATATGGTACTAACATCGGGAGCAACTCCTGCAATTGAGATACTATCGTTCTGCTTGGCAGACCATGGAAATGCATTCCTTGTCCCTACACCTTATTATCCAGGGGACGTAAGATGGCGTCCAGGGATAGACTTAATACCTGTTCACTGTCGAAGTACTGACAATTTCAATCTAAACATCACTGCCCTTGAACAAGCATTTAGTCAAGCAAGAAAACGAGGAGTCAAAGTTCGCGGAATTCTCATCTCGAACCCTTCAAATCCTGTTGGCAATATTCTGACACCAGAAATGCTTTTCAGTCTTCTAGACTTTGCTGAAGATAAAAACATTCATATCATTGCTGACGAAGTATTTGCAGGCTCCACGTACGGAAGCGAGGAGTTTGTGAGTATAGCCGAAGTTCTCGATTCAGAATATATAGATAAAAGCCGCGTTCATATAGTGTACGGCCTATCAAAAGACCTTTCGATTGCTGGATTTAGAGTCGGAGTTATACATTCACACAACAAAACTGTTTTGGATGCTGCCAAAAAAATGAGCAGATTTTCATCTATATCTGCACCAACACAGAGGTTAGTTACTTCAATGCTTTCCGATAAACGATTCATTCAGGAATACATTGAAACCAACCGAAATAGAATAAGACGGGTGCACAATGAGTTTGTGGATTGTTTAACCAAACTAGGAATCAAGTGTGCTAAGAGCAGCGCCGGTATGTTCTGTTGGGCTGATATGAGTGGTTTAATCAGACCTTATAGCGAGAAAGGAGAACTTGAATTATGGGAGAAGTTTATGAGTGTTGCTAAGATAAATATAACTCCTGGATCGGCTTGCCATTGCATAGAACCAGGATGGTTTCGTATTTGTTTTACAACTATATCGTTCGAGGAAATCCCTCTAGTTATTGATAGGATTCGGAAAGTTGTTGAAAGTTGTAAATCTTCCAATTGA |
| Protein sequence |
>MsG0680034122.01.T01 MTQTQKKPTNPKKTQSQPPSSSSSSPSRTGMKLIVPLQAVVQGRNGFLLGSLIPLSLFYFLQLYLKRRRPENNNNNTPSSPTLLPRSSSRSNLSSRGSISSVKVSKLANVVAKRDDSLYYVGIERVEKDPYHVKFNPHGIIQLGLSDNKLSLDLIEKWLVKNLEGSMMGNEDINLSINGIAPYQSFHGLNELKIALADFMHQVMGGSVEFDPSNMVLTSGATPAIEILSFCLADHGNAFLVPTPYYPGDVRWRPGIDLIPVHCRSTDNFNLNITALEQAFSQARKRGVKVRGILISNPSNPVGNILTPEMLFSLLDFAEDKNIHIIADEVFAGSTYGSEEFVSIAEVLDSEYIDKSRVHIVYGLSKDLSIAGFRVGVIHSHNKTVLDAAKKMSRFSSISAPTQRLVTSMLSDKRFIQEYIETNRNRIRRVHNEFVDCLTKLGIKCAKSSAGMFCWADMSGLIRPYSEKGELELWEKFMSVAKINITPGSACHCIEPGWFRICFTTISFEEIPLVIDRIRKVVESCKSSN* |
