AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049016.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049016.01.T01 MTR_4g032805 98.980 98 1 0 1 98 172 269 2.15e-67 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049016.01.T01 AT4G09350 68.041 97 31 0 1 97 150 246 2.41e-48 154

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAGACGATCAACCATATC+TGG 0.335910 contig610end:+10912 MsG0080049016.01.T01:intergenic
TTCTTCAATGCTACCTCATA+TGG 0.345848 contig610end:+10948 MsG0080049016.01.T01:intergenic
TTATGAACCTGTTCCAGATA+TGG 0.348047 contig610end:-10925 MsG0080049016.01.T01:CDS
AAAATTGAAAATAAAATTAG+AGG 0.357068 contig610end:-10973 MsG0080049016.01.T01:CDS
AAAGCAGAAGATTCTATGAT+TGG 0.410574 contig610end:-11031 MsG0080049016.01.T01:CDS
CGATCAACCATATCTGGAAC+AGG 0.472752 contig610end:+10918 MsG0080049016.01.T01:intergenic
AAAATTAGAGGATCCATATG+AGG 0.515032 contig610end:-10961 MsG0080049016.01.T01:CDS
CCAGATATGGTTGATCGTCT+TGG 0.521131 contig610end:-10912 MsG0080049016.01.T01:CDS
TGATTGGACCCTTGCTCAAG+AGG 0.534983 contig610end:-11015 MsG0080049016.01.T01:CDS
GATATGGTTGATCGTCTTGG+TGG 0.546904 contig610end:-10909 MsG0080049016.01.T01:CDS
GACTTGCAACCTCTTGAGCA+AGG 0.555969 contig610end:+11006 MsG0080049016.01.T01:intergenic
ACTTGCAACCTCTTGAGCAA+GGG 0.629898 contig610end:+11007 MsG0080049016.01.T01:intergenic
ATATGGTTGATCGTCTTGGT+GGG 0.630473 contig610end:-10908 MsG0080049016.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTGAAAATAAAATTAG+AGG - contig610end:10895-10914 MsG0080049016.01.T01:CDS 10.0%
!!! TTATTTTCAATTTTTCTTGT+TGG + contig610end:10889-10908 MsG0080049016.01.T01:intergenic 15.0%
AAAATTAGAGGATCCATATG+AGG - contig610end:10907-10926 MsG0080049016.01.T01:CDS 30.0%
AAAGCAGAAGATTCTATGAT+TGG - contig610end:10837-10856 MsG0080049016.01.T01:CDS 30.0%
TTATGAACCTGTTCCAGATA+TGG - contig610end:10943-10962 MsG0080049016.01.T01:CDS 35.0%
TTCTTCAATGCTACCTCATA+TGG + contig610end:10923-10942 MsG0080049016.01.T01:intergenic 35.0%
ATATGGTTGATCGTCTTGGT+GGG - contig610end:10960-10979 MsG0080049016.01.T01:CDS 40.0%
ACTTGCAACCTCTTGAGCAA+GGG + contig610end:10864-10883 MsG0080049016.01.T01:intergenic 45.0%
CCAAGACGATCAACCATATC+TGG + contig610end:10959-10978 MsG0080049016.01.T01:intergenic 45.0%
CCAGATATGGTTGATCGTCT+TGG - contig610end:10956-10975 MsG0080049016.01.T01:CDS 45.0%
GATATGGTTGATCGTCTTGG+TGG - contig610end:10959-10978 MsG0080049016.01.T01:CDS 45.0%
! CGATCAACCATATCTGGAAC+AGG + contig610end:10953-10972 MsG0080049016.01.T01:intergenic 45.0%
GACTTGCAACCTCTTGAGCA+AGG + contig610end:10865-10884 MsG0080049016.01.T01:intergenic 50.0%
TGATTGGACCCTTGCTCAAG+AGG - contig610end:10853-10872 MsG0080049016.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
contig610end gene 10797 11093 10797 ID=MsG0080049016.01;Name=MsG0080049016.01
contig610end mRNA 10797 11093 10797 ID=MsG0080049016.01.T01;Parent=MsG0080049016.01;Name=MsG0080049016.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|98
contig610end exon 10797 11093 10797 ID=MsG0080049016.01.T01:exon:2252;Parent=MsG0080049016.01.T01
contig610end CDS 10797 11093 10797 ID=MsG0080049016.01.T01:cds;Parent=MsG0080049016.01.T01
Gene Sequence

>MsG0080049016.01.T01

ATGAAATTAAAAGAAGTTTACACTGTTCTGAGCAATGAAGAAAGCAGAAGATTCTATGATTGGACCCTTGCTCAAGAGGTTGCAAGTCGCCAACAAGAAAAATTGAAAATAAAATTAGAGGATCCATATGAGGTAGCATTGAAGAATTATGAACCTGTTCCAGATATGGTTGATCGTCTTGGTGGGAAGAATATGAAGCTTAGTGATCAAGCTGTTTCAGCTATTACTATTGATATTTTTATCATTATTTTTGCTATATGTTGTATCACTTATGTAGTTTTCTTTAAAGAATACTGA

Protein sequence

>MsG0080049016.01.T01

MKLKEVYTVLSNEESRRFYDWTLAQEVASRQQEKLKIKLEDPYEVALKNYEPVPDMVDRLGGKNMKLSDQAVSAITIDIFIIIFAICCITYVVFFKEY*