Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048699.01 | MsG0780035951.01 | 0.832244 | 1.056003e-55 | 1.173425e-52 |
| MsG0180003624.01 | MsG0780035951.01 | 0.805779 | 1.132009e-49 | 6.041872e-47 |
| MsG0380013923.01 | MsG0780035951.01 | 0.801530 | 8.622797e-49 | 4.127452e-46 |
| MsG0380013942.01 | MsG0780035951.01 | 0.821113 | 4.798048e-53 | 3.868444e-50 |
| MsG0380013951.01 | MsG0780035951.01 | 0.848311 | 6.645408e-60 | 1.214539e-56 |
| MsG0380013968.01 | MsG0780035951.01 | 0.852266 | 5.168392e-61 | 1.074106e-57 |
| MsG0380014018.01 | MsG0780035951.01 | 0.819093 | 1.392096e-52 | 1.061044e-49 |
| MsG0380014039.01 | MsG0780035951.01 | 0.822852 | 1.897339e-53 | 1.606939e-50 |
| MsG0680034837.01 | MsG0780035951.01 | 0.802551 | 5.316750e-49 | 2.611861e-46 |
| MsG0480021476.01 | MsG0780035951.01 | 0.802552 | 5.313572e-49 | 2.610390e-46 |
| MsG0380016507.01 | MsG0780035951.01 | 0.806642 | 7.450107e-50 | 4.064580e-47 |
| MsG0380017007.01 | MsG0780035951.01 | 0.831754 | 1.395129e-55 | 1.528402e-52 |
| MsG0280010145.01 | MsG0780035951.01 | 0.825007 | 5.927295e-54 | 5.337193e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATGAAGTGGAGTTAATA+TGG | 0.290617 | 7:+458945 | MsG0780035951.01.T01:CDS |
| AGGGAAGTCAATCATAAGTA+TGG | 0.423072 | 7:+459013 | MsG0780035951.01.T01:CDS |
| CTCAAGAGGGTTCTGTGTGT+TGG | 0.444212 | 7:-458989 | None:intergenic |
| AAACGTCAATCCTTGAGTCT+TGG | 0.474340 | 7:-458924 | None:intergenic |
| AGGATTGACGTTTGATGAAG+TGG | 0.485530 | 7:+458934 | MsG0780035951.01.T01:CDS |
| CACACAGAACCCTCTTGAGC+AGG | 0.490389 | 7:+458993 | MsG0780035951.01.T01:CDS |
| GTTAATATGGAAGGAGAGCA+TGG | 0.496231 | 7:+458958 | MsG0780035951.01.T01:CDS |
| CCTTGAGTCTTGGAACATAC+AGG | 0.518821 | 7:-458914 | None:intergenic |
| GCATGGCAGCTACTGTATGT+TGG | 0.540274 | 7:+458835 | MsG0780035951.01.T01:CDS |
| TCGAGGTATATGCGGTGGCA+TGG | 0.583737 | 7:+458818 | None:intergenic |
| CCTGTATGTTCCAAGACTCA+AGG | 0.593405 | 7:+458914 | MsG0780035951.01.T01:CDS |
| ATTGACTTCCCTGCTCAAGA+GGG | 0.595188 | 7:-459002 | None:intergenic |
| TGAAGTGGAGTTAATATGGA+AGG | 0.600864 | 7:+458949 | MsG0780035951.01.T01:CDS |
| TTAATATGGAAGGAGAGCAT+GGG | 0.604194 | 7:+458959 | MsG0780035951.01.T01:CDS |
| GATTGACTTCCCTGCTCAAG+AGG | 0.608237 | 7:-459003 | None:intergenic |
| ACACAGAACCCTCTTGAGCA+GGG | 0.618035 | 7:+458994 | MsG0780035951.01.T01:CDS |
| CCGAGCTGTTATAGCTGTGG+TGG | 0.624007 | 7:+458877 | MsG0780035951.01.T01:CDS |
| CCACCACAGCTATAACAGCT+CGG | 0.639437 | 7:-458877 | None:intergenic |
| GAATATCGAGGTATATGCGG+TGG | 0.648219 | 7:+458813 | None:intergenic |
| TAATATGGAAGGAGAGCATG+GGG | 0.668553 | 7:+458960 | MsG0780035951.01.T01:CDS |
| TGTCCGAGCTGTTATAGCTG+TGG | 0.674348 | 7:+458874 | MsG0780035951.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGATGAAGTGGAGTTAATA+TGG | + | Chr7:458945-458964 | MsG0780035951.01.T01:CDS | 30.0% | |
| AGGGAAGTCAATCATAAGTA+TGG | + | Chr7:459013-459032 | MsG0780035951.01.T01:CDS | 35.0% | |
| TGAAGTGGAGTTAATATGGA+AGG | + | Chr7:458949-458968 | MsG0780035951.01.T01:CDS | 35.0% | |
| ! | TTAATATGGAAGGAGAGCAT+GGG | + | Chr7:458959-458978 | MsG0780035951.01.T01:CDS | 35.0% |
| AAACGTCAATCCTTGAGTCT+TGG | - | Chr7:458927-458946 | None:intergenic | 40.0% | |
| AGGATTGACGTTTGATGAAG+TGG | + | Chr7:458934-458953 | MsG0780035951.01.T01:CDS | 40.0% | |
| GTTAATATGGAAGGAGAGCA+TGG | + | Chr7:458958-458977 | MsG0780035951.01.T01:CDS | 40.0% | |
| ! | TAATATGGAAGGAGAGCATG+GGG | + | Chr7:458960-458979 | MsG0780035951.01.T01:CDS | 40.0% |
| CCTGTATGTTCCAAGACTCA+AGG | + | Chr7:458914-458933 | MsG0780035951.01.T01:CDS | 45.0% | |
| CCTTGAGTCTTGGAACATAC+AGG | - | Chr7:458917-458936 | None:intergenic | 45.0% | |
| ! | ATTGACTTCCCTGCTCAAGA+GGG | - | Chr7:459005-459024 | None:intergenic | 45.0% |
| ACACAGAACCCTCTTGAGCA+GGG | + | Chr7:458994-459013 | MsG0780035951.01.T01:CDS | 50.0% | |
| CCACCACAGCTATAACAGCT+CGG | - | Chr7:458880-458899 | None:intergenic | 50.0% | |
| CTCAAGAGGGTTCTGTGTGT+TGG | - | Chr7:458992-459011 | None:intergenic | 50.0% | |
| GCATGGCAGCTACTGTATGT+TGG | + | Chr7:458835-458854 | MsG0780035951.01.T01:CDS | 50.0% | |
| TGTCCGAGCTGTTATAGCTG+TGG | + | Chr7:458874-458893 | MsG0780035951.01.T01:CDS | 50.0% | |
| ! | GATTGACTTCCCTGCTCAAG+AGG | - | Chr7:459006-459025 | None:intergenic | 50.0% |
| CACACAGAACCCTCTTGAGC+AGG | + | Chr7:458993-459012 | MsG0780035951.01.T01:CDS | 55.0% | |
| CCGAGCTGTTATAGCTGTGG+TGG | + | Chr7:458877-458896 | MsG0780035951.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 458827 | 459045 | 458827 | ID=MsG0780035951.01;Name=MsG0780035951.01 |
| Chr7 | mRNA | 458827 | 459045 | 458827 | ID=MsG0780035951.01.T01;Parent=MsG0780035951.01;Name=MsG0780035951.01.T01;_AED=0.48;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|72 |
| Chr7 | exon | 458827 | 459045 | 458827 | ID=MsG0780035951.01.T01:exon:15425;Parent=MsG0780035951.01.T01 |
| Chr7 | CDS | 458827 | 459045 | 458827 | ID=MsG0780035951.01.T01:cds;Parent=MsG0780035951.01.T01 |
| Gene Sequence |
| Protein sequence |
