Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013968.01.T01 | YP_009027080.1 | 99.736 | 379 | 1 | 0 | 1 | 379 | 15 | 393 | 0 | 774 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013968.01.T01 | B5LMS0 | 98.417 | 379 | 6 | 0 | 1 | 379 | 15 | 393 | 0 | 770 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013968.01.T01 | A0A023HPE3 | 99.736 | 379 | 1 | 0 | 1 | 379 | 15 | 393 | 0.0 | 774 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0380013968.01 | 0.815705 | 8.062158e-52 | 5.599388e-49 |
| MsG0080048302.01 | MsG0380013968.01 | -0.845940 | 2.967110e-59 | 5.025833e-56 |
| MsG0080048560.01 | MsG0380013968.01 | 0.815123 | 1.085910e-51 | 7.422204e-49 |
| MsG0080048690.01 | MsG0380013968.01 | 0.868975 | 4.378044e-66 | 1.615000e-62 |
| MsG0080048691.01 | MsG0380013968.01 | 0.819910 | 9.063406e-53 | 7.067284e-50 |
| MsG0080048692.01 | MsG0380013968.01 | 0.935923 | 3.945343e-97 | 3.265343e-92 |
| MsG0080048693.01 | MsG0380013968.01 | 0.867205 | 1.625072e-65 | 5.626470e-62 |
| MsG0080048694.01 | MsG0380013968.01 | 0.817780 | 2.760961e-52 | 2.029658e-49 |
| MsG0080048695.01 | MsG0380013968.01 | 0.816953 | 4.239719e-52 | 3.047082e-49 |
| MsG0080048697.01 | MsG0380013968.01 | 0.813546 | 2.423568e-51 | 1.587390e-48 |
| MsG0080048698.01 | MsG0380013968.01 | 0.929577 | 5.691568e-93 | 3.205172e-88 |
| MsG0080048699.01 | MsG0380013968.01 | 0.968810 | 3.282910e-129 | 2.828660e-123 |
| MsG0080048813.01 | MsG0380013968.01 | 0.814308 | 1.645852e-51 | 1.100537e-48 |
| MsG0080049076.01 | MsG0380013968.01 | 0.872847 | 2.321951e-67 | 9.861965e-64 |
| MsG0180000337.01 | MsG0380013968.01 | -0.819323 | 1.233740e-52 | 9.462854e-50 |
| MsG0180001072.01 | MsG0380013968.01 | 0.818058 | 2.389949e-52 | 1.770253e-49 |
| MsG0180001925.01 | MsG0380013968.01 | 0.836874 | 7.245708e-57 | 9.251917e-54 |
| MsG0180002214.01 | MsG0380013968.01 | 0.822085 | 2.860013e-53 | 2.369747e-50 |
| MsG0180002226.01 | MsG0380013968.01 | 0.807046 | 6.119431e-50 | 3.373821e-47 |
| MsG0180002439.01 | MsG0380013968.01 | 0.826325 | 2.886753e-54 | 2.698503e-51 |
| MsG0180002546.01 | MsG0380013968.01 | 0.832507 | 9.086166e-56 | 1.017697e-52 |
| MsG0180004181.01 | MsG0380013968.01 | -0.801753 | 7.760386e-49 | 3.735552e-46 |
| MsG0180004301.01 | MsG0380013968.01 | 0.817015 | 4.106246e-52 | 2.956049e-49 |
| MsG0180004435.01 | MsG0380013968.01 | 0.857506 | 1.558000e-62 | 3.854137e-59 |
| MsG0180004506.01 | MsG0380013968.01 | 0.821094 | 4.846374e-53 | 3.905453e-50 |
| MsG0180004761.01 | MsG0380013968.01 | 0.838985 | 2.077052e-57 | 2.828742e-54 |
| MsG0180005791.01 | MsG0380013968.01 | 0.826405 | 2.762857e-54 | 2.588720e-51 |
| MsG0280010695.01 | MsG0380013968.01 | 0.863447 | 2.473162e-64 | 7.499013e-61 |
| MsG0280010780.01 | MsG0380013968.01 | 0.802715 | 4.918942e-49 | 2.426651e-46 |
| MsG0280011035.01 | MsG0380013968.01 | 0.803295 | 3.731174e-49 | 1.868074e-46 |
| MsG0280011120.01 | MsG0380013968.01 | 0.815641 | 8.332251e-52 | 5.776982e-49 |
| MsG0380012366.01 | MsG0380013968.01 | -0.817129 | 3.870103e-52 | 2.794607e-49 |
| MsG0380013448.01 | MsG0380013968.01 | 0.892365 | 1.697985e-74 | 1.547369e-70 |
| MsG0380013888.01 | MsG0380013968.01 | 0.861778 | 8.076755e-64 | 2.311254e-60 |
| MsG0380013890.01 | MsG0380013968.01 | 0.811112 | 8.237852e-51 | 5.055698e-48 |
| MsG0380013892.01 | MsG0380013968.01 | 0.842030 | 3.315986e-58 | 4.963305e-55 |
| MsG0380013916.01 | MsG0380013968.01 | 0.871390 | 7.089637e-67 | 2.855037e-63 |
| MsG0380013917.01 | MsG0380013968.01 | 0.880208 | 6.634437e-70 | 3.705297e-66 |
| MsG0380013920.01 | MsG0380013968.01 | 0.865380 | 6.157229e-65 | 1.999061e-61 |
| MsG0380013921.01 | MsG0380013968.01 | 0.824399 | 8.244058e-54 | 7.293567e-51 |
| MsG0380013923.01 | MsG0380013968.01 | 0.927722 | 7.909252e-92 | 4.008444e-87 |
| MsG0380013926.01 | MsG0380013968.01 | 0.901441 | 2.671655e-78 | 3.610362e-74 |
| MsG0380013928.01 | MsG0380013968.01 | 0.810248 | 1.266955e-50 | 7.598861e-48 |
| MsG0380013929.01 | MsG0380013968.01 | 0.920332 | 1.462893e-87 | 4.930662e-83 |
| MsG0380013931.01 | MsG0380013968.01 | 0.907551 | 4.482286e-81 | 7.989673e-77 |
| MsG0380013932.01 | MsG0380013968.01 | 0.828099 | 1.085337e-54 | 1.068208e-51 |
| MsG0380013940.01 | MsG0380013968.01 | 0.901384 | 2.830997e-78 | 3.814771e-74 |
| MsG0380013941.01 | MsG0380013968.01 | 0.921666 | 2.668027e-88 | 9.667917e-84 |
| MsG0380013942.01 | MsG0380013968.01 | 0.962522 | 5.602522e-121 | 2.815039e-115 |
| MsG0380013943.01 | MsG0380013968.01 | 0.905450 | 4.239752e-80 | 6.861476e-76 |
| MsG0380013944.01 | MsG0380013968.01 | 0.935025 | 1.620720e-96 | 1.274506e-91 |
| MsG0380013945.01 | MsG0380013968.01 | 0.903456 | 3.403714e-79 | 5.043127e-75 |
| MsG0380013946.01 | MsG0380013968.01 | 0.802241 | 6.158201e-49 | 3.001535e-46 |
| MsG0380013948.01 | MsG0380013968.01 | 0.859387 | 4.284777e-63 | 1.129748e-59 |
| MsG0380013951.01 | MsG0380013968.01 | 0.998534 | 5.753980e-268 | 1.700745e-260 |
| MsG0380013952.01 | MsG0380013968.01 | 0.910162 | 2.549578e-82 | 5.139583e-78 |
| MsG0380013953.01 | MsG0380013968.01 | 0.901264 | 3.195538e-78 | 4.283405e-74 |
| MsG0380013954.01 | MsG0380013968.01 | 0.833261 | 5.904775e-56 | 6.764121e-53 |
| MsG0380013956.01 | MsG0380013968.01 | 0.844185 | 8.840784e-59 | 1.416102e-55 |
| MsG0380013957.01 | MsG0380013968.01 | 0.901027 | 4.057474e-78 | 5.378746e-74 |
| MsG0380013961.01 | MsG0380013968.01 | 0.853121 | 2.946769e-61 | 6.299055e-58 |
| MsG0380013962.01 | MsG0380013968.01 | 0.870740 | 1.161106e-66 | 4.565815e-63 |
| MsG0380013966.01 | MsG0380013968.01 | 0.944231 | 2.848172e-103 | 4.027175e-98 |
| MsG0380013967.01 | MsG0380013968.01 | 0.901183 | 3.466259e-78 | 4.629311e-74 |
| MsG0380013968.01 | MsG0380013969.01 | 0.909755 | 4.010185e-82 | 7.919804e-78 |
| MsG0380013968.01 | MsG0380013971.01 | 0.878737 | 2.204272e-69 | 1.163912e-65 |
| MsG0380013968.01 | MsG0380013972.01 | 0.914100 | 2.846688e-84 | 6.957602e-80 |
| MsG0380013968.01 | MsG0380013975.01 | 0.840257 | 9.697134e-58 | 1.373550e-54 |
| MsG0380013968.01 | MsG0380013977.01 | 0.901039 | 4.005845e-78 | 5.313205e-74 |
| MsG0380013968.01 | MsG0380013981.01 | 0.869242 | 3.585811e-66 | 1.336132e-62 |
| MsG0380013968.01 | MsG0380013986.01 | 0.804515 | 2.082122e-49 | 1.075605e-46 |
| MsG0380013968.01 | MsG0380013995.01 | 0.875142 | 3.888253e-68 | 1.796285e-64 |
| MsG0380013968.01 | MsG0380013997.01 | 0.808032 | 3.779254e-50 | 2.137886e-47 |
| MsG0380013968.01 | MsG0380013999.01 | 0.827404 | 1.593922e-54 | 1.537435e-51 |
| MsG0380013968.01 | MsG0380014004.01 | 0.836248 | 1.045870e-56 | 1.310751e-53 |
| MsG0380013968.01 | MsG0380014005.01 | 0.903416 | 3.549718e-79 | 5.250530e-75 |
| MsG0380013968.01 | MsG0380014006.01 | 0.904479 | 1.175811e-79 | 1.822625e-75 |
| MsG0380013968.01 | MsG0380014008.01 | 0.908769 | 1.190360e-81 | 2.244708e-77 |
| MsG0380013968.01 | MsG0380014017.01 | 0.837965 | 3.807867e-57 | 5.027406e-54 |
| MsG0380013968.01 | MsG0380014018.01 | 0.954726 | 1.541547e-112 | 4.446271e-107 |
| MsG0380013968.01 | MsG0380014020.01 | 0.864489 | 1.172284e-64 | 3.687094e-61 |
| MsG0380013968.01 | MsG0380014021.01 | 0.841881 | 3.631161e-58 | 5.409838e-55 |
| MsG0380013968.01 | MsG0380014023.01 | 0.924737 | 4.721337e-90 | 2.026322e-85 |
| MsG0380013968.01 | MsG0380014026.01 | 0.925714 | 1.260798e-90 | 5.708079e-86 |
| MsG0380013968.01 | MsG0380014029.01 | 0.807896 | 4.039681e-50 | 2.276952e-47 |
| MsG0380013968.01 | MsG0380014032.01 | 0.907858 | 3.215030e-81 | 5.810650e-77 |
| MsG0380013968.01 | MsG0380014033.01 | 0.930518 | 1.456943e-93 | 8.688333e-89 |
| MsG0380013968.01 | MsG0380014034.01 | 0.820097 | 8.211785e-53 | 6.437230e-50 |
| MsG0380013968.01 | MsG0380014036.01 | 0.838952 | 2.117841e-57 | 2.881360e-54 |
| MsG0380013968.01 | MsG0380014039.01 | 0.952530 | 1.986941e-110 | 4.895894e-105 |
| MsG0380013968.01 | MsG0380014040.01 | 0.928174 | 4.190142e-92 | 2.175979e-87 |
| MsG0380013968.01 | MsG0380014044.01 | 0.815359 | 9.628362e-52 | 6.623853e-49 |
| MsG0380013968.01 | MsG0380014051.01 | 0.941346 | 4.880789e-101 | 5.679724e-96 |
| MsG0380013968.01 | MsG0380014054.01 | 0.800166 | 1.636873e-48 | 7.569113e-46 |
| MsG0380013968.01 | MsG0380014057.01 | 0.888641 | 4.929024e-73 | 3.849956e-69 |
| MsG0380013968.01 | MsG0380014129.01 | 0.804442 | 2.156112e-49 | 1.111787e-46 |
| MsG0380013968.01 | MsG0380015348.01 | 0.811100 | 8.287338e-51 | 5.084458e-48 |
| MsG0380013968.01 | MsG0380015697.01 | 0.816909 | 4.336610e-52 | 3.112713e-49 |
| MsG0380013968.01 | MsG0380017007.01 | 0.980164 | 1.368518e-149 | 3.164477e-143 |
| MsG0380013968.01 | MsG0380017405.01 | 0.802146 | 6.444167e-49 | 3.133225e-46 |
| MsG0380013968.01 | MsG0380018047.01 | 0.800206 | 1.606744e-48 | 7.437461e-46 |
| MsG0380013968.01 | MsG0480018296.01 | 0.922184 | 1.366863e-88 | 5.093908e-84 |
| MsG0380013968.01 | MsG0480020052.01 | 0.882567 | 9.348848e-71 | 5.722212e-67 |
| MsG0380013968.01 | MsG0480021327.01 | 0.809866 | 1.530967e-50 | 9.091292e-48 |
| MsG0380013968.01 | MsG0480021331.01 | 0.828058 | 1.110325e-54 | 1.091492e-51 |
| MsG0380013968.01 | MsG0480021476.01 | 0.843819 | 1.108532e-58 | 1.755212e-55 |
| MsG0380013968.01 | MsG0480022125.01 | -0.846557 | 2.015977e-59 | 3.483323e-56 |
| MsG0380013968.01 | MsG0480022508.01 | -0.818844 | 1.585360e-52 | 1.200156e-49 |
| MsG0380013968.01 | MsG0480022591.01 | 0.810505 | 1.115092e-50 | 6.733464e-48 |
| MsG0380013968.01 | MsG0480023810.01 | -0.840664 | 7.589955e-58 | 1.088688e-54 |
| MsG0380013968.01 | MsG0580024570.01 | 0.871967 | 4.561982e-67 | 1.875589e-63 |
| MsG0380013968.01 | MsG0580027677.01 | 0.888742 | 4.506785e-73 | 3.534122e-69 |
| MsG0380013968.01 | MsG0580028051.01 | -0.814637 | 1.391918e-51 | 9.390311e-49 |
| MsG0380013968.01 | MsG0580029583.01 | -0.854851 | 9.346164e-62 | 2.116177e-58 |
| MsG0380013968.01 | MsG0680030439.01 | 0.802841 | 4.631672e-49 | 2.292390e-46 |
| MsG0380013968.01 | MsG0680035741.01 | -0.804321 | 2.284616e-49 | 1.174417e-46 |
| MsG0380013968.01 | MsG0780035951.01 | 0.852266 | 5.168392e-61 | 1.074106e-57 |
| MsG0380013968.01 | MsG0780038197.01 | 0.875667 | 2.572959e-68 | 1.211730e-64 |
| MsG0380013968.01 | MsG0780039190.01 | -0.817826 | 2.696574e-52 | 1.984813e-49 |
| MsG0380013968.01 | MsG0780040060.01 | -0.844405 | 7.718003e-59 | 1.244653e-55 |
| MsG0380013968.01 | MsG0780040316.01 | 0.811510 | 6.754372e-51 | 4.189071e-48 |
| MsG0380013968.01 | MsG0780040440.01 | -0.824413 | 8.181749e-54 | 7.241297e-51 |
| MsG0380013968.01 | MsG0780040480.01 | -0.841823 | 3.761268e-58 | 5.593417e-55 |
| MsG0380013968.01 | MsG0880042291.01 | 0.825855 | 3.733381e-54 | 3.443877e-51 |
| MsG0380013968.01 | MsG0880042368.01 | -0.817039 | 4.055248e-52 | 2.921192e-49 |
| MsG0380013968.01 | MsG0880043246.01 | -0.825994 | 3.459939e-54 | 3.204156e-51 |
| MsG0380013968.01 | MsG0880043824.01 | -0.821278 | 4.395770e-53 | 3.560180e-50 |
| MsG0380013968.01 | MsG0880044669.01 | -0.801922 | 7.164673e-49 | 3.463717e-46 |
| MsG0380013968.01 | MsG0880045097.01 | 0.819600 | 1.066503e-52 | 8.244108e-50 |
| MsG0380013968.01 | MsG0880045630.01 | 0.882824 | 7.534957e-71 | 4.659673e-67 |
| MsG0380013968.01 | MsG0880045861.01 | -0.820133 | 8.058919e-53 | 6.323537e-50 |
| MsG0380013968.01 | MsG0880046099.01 | 0.832788 | 7.739178e-56 | 8.740800e-53 |
| MsG0380013968.01 | MsG0880047081.01 | -0.820795 | 5.678371e-53 | 4.537744e-50 |
| MsG0280006475.01 | MsG0380013968.01 | 0.855931 | 4.530130e-62 | 1.063203e-58 |
| MsG0280006704.01 | MsG0380013968.01 | -0.841704 | 4.043862e-58 | 5.990758e-55 |
| MsG0280007759.01 | MsG0380013968.01 | -0.815674 | 8.192342e-52 | 5.684966e-49 |
| MsG0280007760.01 | MsG0380013968.01 | -0.813814 | 2.115648e-51 | 1.395780e-48 |
| MsG0280009971.01 | MsG0380013968.01 | -0.901181 | 3.471875e-78 | 4.636629e-74 |
| MsG0280010137.01 | MsG0380013968.01 | 0.822895 | 1.854128e-53 | 1.572211e-50 |
| MsG0280010140.01 | MsG0380013968.01 | 0.820439 | 6.856783e-53 | 5.425585e-50 |
| MsG0280010145.01 | MsG0380013968.01 | 0.962168 | 1.476093e-120 | 7.219345e-115 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013968.01.T01 | MTR_4g006100 | 98.656 | 372 | 5 | 0 | 1 | 372 | 15 | 386 | 0.0 | 761 |
| MsG0380013968.01.T01 | MTR_6g042200 | 98.118 | 372 | 7 | 0 | 1 | 372 | 15 | 386 | 0.0 | 756 |
| MsG0380013968.01.T01 | MTR_0002s0150 | 83.912 | 317 | 51 | 0 | 56 | 372 | 101 | 417 | 0.0 | 570 |
| MsG0380013968.01.T01 | MTR_2g451380 | 73.934 | 211 | 4 | 1 | 1 | 211 | 15 | 174 | 5.36e-100 | 294 |
| MsG0380013968.01.T01 | MTR_7g021700 | 100.000 | 83 | 0 | 0 | 1 | 83 | 15 | 97 | 3.57e-50 | 178 |
| MsG0380013968.01.T01 | MTR_1g099540 | 89.041 | 73 | 8 | 0 | 114 | 186 | 3 | 75 | 5.01e-42 | 142 |
| MsG0380013968.01.T01 | MTR_4g117480 | 94.286 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 2.38e-41 | 142 |
| MsG0380013968.01.T01 | MTR_8g035600 | 90.909 | 66 | 6 | 0 | 184 | 249 | 33 | 98 | 7.55e-37 | 129 |
| MsG0380013968.01.T01 | MTR_6g011030 | 80.000 | 40 | 7 | 1 | 140 | 179 | 45 | 83 | 1.37e-15 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013968.01.T01 | ATCG01110 | 89.974 | 379 | 38 | 0 | 1 | 379 | 15 | 393 | 0.0 | 716 |
| MsG0380013968.01.T01 | ATMG00510 | 40.000 | 385 | 218 | 3 | 2 | 379 | 16 | 394 | 2.35e-94 | 288 |
Find 72 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAGTTAAAAGAATGAAAT+TGG | 0.240964 | 3:+48533076 | MsG0380013968.01.T01:CDS |
| GGAGAGAAGAAGTTATAAAT+TGG | 0.256563 | 3:+48532604 | MsG0380013968.01.T01:CDS |
| TATTGATTGTGAACCAATAT+TGG | 0.291835 | 3:+48532086 | MsG0380013968.01.T01:CDS |
| TAAAATTATTCAACAGGCTT+TGG | 0.308807 | 3:+48532791 | MsG0380013968.01.T01:CDS |
| ACAGAAGCAATAACCGTAAA+TGG | 0.315370 | 3:+48532207 | MsG0380013968.01.T01:CDS |
| CAATTGTTCCGGTCCATTTA+CGG | 0.318728 | 3:-48532220 | None:intergenic |
| CGTAAATGGACCGGAACAAT+TGG | 0.319748 | 3:+48532221 | MsG0380013968.01.T01:CDS |
| TCTCACATAAAGTTCTTGTT+TGG | 0.322955 | 3:-48532940 | None:intergenic |
| GGTTATCAGGACCGATGCTT+CGG | 0.326389 | 3:+48532628 | MsG0380013968.01.T01:CDS |
| ATCCATTAAAATTATTCAAC+AGG | 0.326815 | 3:+48532785 | MsG0380013968.01.T01:CDS |
| TATTCAAAATCATTCCATTC+TGG | 0.326932 | 3:-48532876 | None:intergenic |
| GGACCGATGCTTCGGGCTTC+CGG | 0.333395 | 3:+48532636 | MsG0380013968.01.T01:CDS |
| CTGTGTAAATAACCCAATAT+TGG | 0.346800 | 3:-48532099 | None:intergenic |
| GAGAGAAGAAGTTATAAATT+GGG | 0.349483 | 3:+48532605 | MsG0380013968.01.T01:CDS |
| AGGTAGCATAGATATCATTA+TGG | 0.351209 | 3:+48533118 | MsG0380013968.01.T01:CDS |
| CAGGCTTTGGAAGGAATTCC+AGG | 0.354309 | 3:+48532804 | MsG0380013968.01.T01:CDS |
| TGAATATTTCCCAATTGTTC+CGG | 0.368041 | 3:-48532231 | None:intergenic |
| GCACCAATATCTGCCATAAA+AGG | 0.383520 | 3:-48532333 | None:intergenic |
| ATTGATTGTGAACCAATATT+GGG | 0.384432 | 3:+48532087 | MsG0380013968.01.T01:CDS |
| GTTATCAGGACCGATGCTTC+GGG | 0.385864 | 3:+48532629 | MsG0380013968.01.T01:CDS |
| GCTCGTTATTTAGTTCGAAT+TGG | 0.386457 | 3:+48532750 | MsG0380013968.01.T01:CDS |
| ACGTAGATCCCATTGTATTC+CGG | 0.396087 | 3:-48532655 | None:intergenic |
| CTCACATAAAGTTCTTGTTT+GGG | 0.400339 | 3:-48532939 | None:intergenic |
| CGAAGAACACCATGCATTGA+TGG | 0.409727 | 3:-48532033 | None:intergenic |
| GCAGATATTGTATAATTGTT+CGG | 0.421110 | 3:-48532146 | None:intergenic |
| AGCTTCTCATCTACTATGGC+TGG | 0.422896 | 3:+48532308 | MsG0380013968.01.T01:CDS |
| GTTGAAGGCGTAGGTGTTGT+TGG | 0.429176 | 3:+48532582 | MsG0380013968.01.T01:CDS |
| CGACTTATTGTTACTCTGGA+TGG | 0.439520 | 3:+48532054 | MsG0380013968.01.T01:CDS |
| GCATAGCTTCTCATCTACTA+TGG | 0.439604 | 3:+48532304 | MsG0380013968.01.T01:CDS |
| GTAGCGGCTGATCTACCTTA+TGG | 0.440758 | 3:+48532462 | MsG0380013968.01.T01:CDS |
| AGTTCGAATTGGTGAAATGA+TGG | 0.442828 | 3:+48532761 | MsG0380013968.01.T01:CDS |
| AATTTGATTGGGAAGTTCAA+TGG | 0.448549 | 3:+48532706 | MsG0380013968.01.T01:CDS |
| AGTGTTACGAGGAATTTGAT+TGG | 0.450881 | 3:+48532694 | MsG0380013968.01.T01:CDS |
| GGTAGCATAGATATCATTAT+GGG | 0.462662 | 3:+48533119 | MsG0380013968.01.T01:CDS |
| TCTTCGACTTATTGTTACTC+TGG | 0.475804 | 3:+48532050 | MsG0380013968.01.T01:CDS |
| TTGAAGGCGTAGGTGTTGTT+GGG | 0.476016 | 3:+48532583 | MsG0380013968.01.T01:CDS |
| CATAATTACTCGTATATAGC+TGG | 0.480165 | 3:-48532268 | None:intergenic |
| TCGCTCCTTTGATAGAGAAA+AGG | 0.480513 | 3:+48532851 | MsG0380013968.01.T01:CDS |
| TAATCCCAACGTGTTACATA+AGG | 0.485650 | 3:-48532168 | None:intergenic |
| GTGTTACGAGGAATTTGATT+GGG | 0.487456 | 3:+48532695 | MsG0380013968.01.T01:CDS |
| AGAGAAGAAGTTATAAATTG+GGG | 0.491460 | 3:+48532606 | MsG0380013968.01.T01:CDS |
| GGGTTATTTACACAGAGGTA+TGG | 0.494293 | 3:+48532107 | MsG0380013968.01.T01:CDS |
| TCTGCCTTATGTAACACGTT+GGG | 0.505724 | 3:+48532164 | MsG0380013968.01.T01:CDS |
| TCGGGCTTCCGGAATACAAT+GGG | 0.512104 | 3:+48532647 | MsG0380013968.01.T01:CDS |
| GCTGATATTATGACAATACT+AGG | 0.522631 | 3:+48533098 | MsG0380013968.01.T01:CDS |
| GTAAATGGACCGGAACAATT+GGG | 0.525773 | 3:+48532222 | MsG0380013968.01.T01:CDS |
| ATAGAGAAAAGGAGCCAGAA+TGG | 0.528576 | 3:+48532862 | MsG0380013968.01.T01:CDS |
| AGATGGAAAATTCGTCCACC+TGG | 0.529045 | 3:+48533026 | MsG0380013968.01.T01:CDS |
| CATTTATCTATCCAACCATA+AGG | 0.536061 | 3:-48532477 | None:intergenic |
| GATCTATTTGAAGCTGCGAC+AGG | 0.538812 | 3:+48532402 | MsG0380013968.01.T01:CDS |
| ATTATTCAACAGGCTTTGGA+AGG | 0.539344 | 3:+48532795 | MsG0380013968.01.T01:CDS |
| CGGCTGATCTACCTTATGGT+TGG | 0.543311 | 3:+48532466 | MsG0380013968.01.T01:CDS |
| AGCAATAACCGTAAATGGAC+CGG | 0.546967 | 3:+48532212 | MsG0380013968.01.T01:CDS |
| TGTTTGGGCAATTCAAAAGT+CGG | 0.557531 | 3:-48532924 | None:intergenic |
| TTTCTAATAGGAGATCAGAA+TGG | 0.560048 | 3:+48532993 | MsG0380013968.01.T01:CDS |
| TTAGAACGAGTTGAAGGCGT+AGG | 0.570320 | 3:+48532573 | MsG0380013968.01.T01:CDS |
| ATTTGCAAATTGATAAAACC+AGG | 0.570420 | 3:-48533044 | None:intergenic |
| GAAGAACACCATGCATTGAT+GGG | 0.574286 | 3:-48532032 | None:intergenic |
| ATCTGCCTTATGTAACACGT+TGG | 0.575287 | 3:+48532163 | MsG0380013968.01.T01:CDS |
| ATTCCGGAAGCCCGAAGCAT+CGG | 0.578734 | 3:-48532639 | None:intergenic |
| CTATATACGAGTAATTATGT+TGG | 0.584312 | 3:+48532272 | MsG0380013968.01.T01:CDS |
| GCTTCTCATCTACTATGGCT+GGG | 0.589546 | 3:+48532309 | MsG0380013968.01.T01:CDS |
| TTCGGGCTTCCGGAATACAA+TGG | 0.596428 | 3:+48532646 | MsG0380013968.01.T01:CDS |
| CCATGCATTGATGGGTGTTG+AGG | 0.621910 | 3:-48532024 | None:intergenic |
| CCTCAACACCCATCAATGCA+TGG | 0.624983 | 3:+48532024 | MsG0380013968.01.T01:CDS |
| AGAACTTTATGTGAGAGTTG+AGG | 0.641734 | 3:+48532947 | MsG0380013968.01.T01:CDS |
| GAGAAGAAGTTATAAATTGG+GGG | 0.651461 | 3:+48532607 | MsG0380013968.01.T01:CDS |
| CTTTGGAAGGAATTCCAGGT+GGG | 0.666113 | 3:+48532808 | MsG0380013968.01.T01:CDS |
| TGCAAATTGATAAAACCAGG+TGG | 0.677686 | 3:-48533041 | None:intergenic |
| GCTTTGGAAGGAATTCCAGG+TGG | 0.686796 | 3:+48532807 | MsG0380013968.01.T01:CDS |
| CGATAATTATGAGTGTTACG+AGG | 0.689833 | 3:+48532683 | MsG0380013968.01.T01:CDS |
| ATATTGGGTTATTTACACAG+AGG | 0.748960 | 3:+48532102 | MsG0380013968.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAGTTAAAAGAATGAAAT+TGG | + | Chr3:48533076-48533095 | MsG0380013968.01.T01:CDS | 15.0% |
| !!! | TCTCTAAAAATATAGAAAAA+GGG | - | Chr3:48532366-48532385 | None:intergenic | 15.0% |
| !!! | TTCTCTAAAAATATAGAAAA+AGG | - | Chr3:48532367-48532386 | None:intergenic | 15.0% |
| !!! | TTGCAATTATTTTTTAACAA+GGG | + | Chr3:48532509-48532528 | MsG0380013968.01.T01:CDS | 15.0% |
| !!! | TTTGCAATTATTTTTTAACA+AGG | + | Chr3:48532508-48532527 | MsG0380013968.01.T01:CDS | 15.0% |
| !! | ATCCATTAAAATTATTCAAC+AGG | + | Chr3:48532785-48532804 | MsG0380013968.01.T01:CDS | 20.0% |
| !! | TCTAAAAATATAGAAAAAGG+GGG | - | Chr3:48532364-48532383 | None:intergenic | 20.0% |
| !!! | ATGCATAATTATTTTCGTAT+TGG | + | Chr3:48532435-48532454 | MsG0380013968.01.T01:CDS | 20.0% |
| !!! | CTCTAAAAATATAGAAAAAG+GGG | - | Chr3:48532365-48532384 | None:intergenic | 20.0% |
| !!! | GAATTAGGAATTTTTCTAAT+AGG | + | Chr3:48532981-48533000 | MsG0380013968.01.T01:CDS | 20.0% |
| !!! | TTCATTCTTTTAACTAATTG+AGG | - | Chr3:48533074-48533093 | None:intergenic | 20.0% |
| ! | AGAGAAGAAGTTATAAATTG+GGG | + | Chr3:48532606-48532625 | MsG0380013968.01.T01:CDS | 25.0% |
| ! | ATTGATTGTGAACCAATATT+GGG | + | Chr3:48532087-48532106 | MsG0380013968.01.T01:CDS | 25.0% |
| ! | ATTTGCAAATTGATAAAACC+AGG | - | Chr3:48533047-48533066 | None:intergenic | 25.0% |
| ! | CTATATACGAGTAATTATGT+TGG | + | Chr3:48532272-48532291 | MsG0380013968.01.T01:CDS | 25.0% |
| ! | GAGAGAAGAAGTTATAAATT+GGG | + | Chr3:48532605-48532624 | MsG0380013968.01.T01:CDS | 25.0% |
| ! | GCAGATATTGTATAATTGTT+CGG | - | Chr3:48532149-48532168 | None:intergenic | 25.0% |
| ! | TAAAATTATTCAACAGGCTT+TGG | + | Chr3:48532791-48532810 | MsG0380013968.01.T01:CDS | 25.0% |
| ! | TATTCAAAATCATTCCATTC+TGG | - | Chr3:48532879-48532898 | None:intergenic | 25.0% |
| !! | AGCCTGTTGAATAATTTTAA+TGG | - | Chr3:48532790-48532809 | None:intergenic | 25.0% |
| !! | CATAATTATTTTCGTATTGG+AGG | + | Chr3:48532438-48532457 | MsG0380013968.01.T01:CDS | 25.0% |
| !! | TATTGATTGTGAACCAATAT+TGG | + | Chr3:48532086-48532105 | MsG0380013968.01.T01:CDS | 25.0% |
| !!! | ATTTTTTTAGAACGAGTTGA+AGG | + | Chr3:48532567-48532586 | MsG0380013968.01.T01:CDS | 25.0% |
| !!! | GATTTTGAATATCGATTCAT+TGG | + | Chr3:48532888-48532907 | MsG0380013968.01.T01:CDS | 25.0% |
| !!! | TCAACTCGTTCTAAAAAAAT+TGG | - | Chr3:48532567-48532586 | None:intergenic | 25.0% |
| AATTTGATTGGGAAGTTCAA+TGG | + | Chr3:48532706-48532725 | MsG0380013968.01.T01:CDS | 30.0% | |
| AGGTAGCATAGATATCATTA+TGG | + | Chr3:48533118-48533137 | MsG0380013968.01.T01:CDS | 30.0% | |
| ATATTGGGTTATTTACACAG+AGG | + | Chr3:48532102-48532121 | MsG0380013968.01.T01:CDS | 30.0% | |
| CATAATTACTCGTATATAGC+TGG | - | Chr3:48532271-48532290 | None:intergenic | 30.0% | |
| CATTTATCTATCCAACCATA+AGG | - | Chr3:48532480-48532499 | None:intergenic | 30.0% | |
| CTGTGTAAATAACCCAATAT+TGG | - | Chr3:48532102-48532121 | None:intergenic | 30.0% | |
| GAGAAGAAGTTATAAATTGG+GGG | + | Chr3:48532607-48532626 | MsG0380013968.01.T01:CDS | 30.0% | |
| GCTGATATTATGACAATACT+AGG | + | Chr3:48533098-48533117 | MsG0380013968.01.T01:CDS | 30.0% | |
| GGAGAGAAGAAGTTATAAAT+TGG | + | Chr3:48532604-48532623 | MsG0380013968.01.T01:CDS | 30.0% | |
| GGTAGCATAGATATCATTAT+GGG | + | Chr3:48533119-48533138 | MsG0380013968.01.T01:CDS | 30.0% | |
| TCTCACATAAAGTTCTTGTT+TGG | - | Chr3:48532943-48532962 | None:intergenic | 30.0% | |
| TGAATATTTCCCAATTGTTC+CGG | - | Chr3:48532234-48532253 | None:intergenic | 30.0% | |
| TTTCTAATAGGAGATCAGAA+TGG | + | Chr3:48532993-48533012 | MsG0380013968.01.T01:CDS | 30.0% | |
| ! | CTCACATAAAGTTCTTGTTT+GGG | - | Chr3:48532942-48532961 | None:intergenic | 30.0% |
| AAAAATTCCTAATTCCCCCT+TGG | - | Chr3:48532976-48532995 | None:intergenic | 35.0% | |
| AAAATTCCTAATTCCCCCTT+GGG | - | Chr3:48532975-48532994 | None:intergenic | 35.0% | |
| ACAGAAGCAATAACCGTAAA+TGG | + | Chr3:48532207-48532226 | MsG0380013968.01.T01:CDS | 35.0% | |
| AGAACTTTATGTGAGAGTTG+AGG | + | Chr3:48532947-48532966 | MsG0380013968.01.T01:CDS | 35.0% | |
| AGTGTTACGAGGAATTTGAT+TGG | + | Chr3:48532694-48532713 | MsG0380013968.01.T01:CDS | 35.0% | |
| AGTTCGAATTGGTGAAATGA+TGG | + | Chr3:48532761-48532780 | MsG0380013968.01.T01:CDS | 35.0% | |
| CGATAATTATGAGTGTTACG+AGG | + | Chr3:48532683-48532702 | MsG0380013968.01.T01:CDS | 35.0% | |
| GAAGTTCAATGGCAAAAAGA+AGG | + | Chr3:48532717-48532736 | MsG0380013968.01.T01:CDS | 35.0% | |
| GTGTTACGAGGAATTTGATT+GGG | + | Chr3:48532695-48532714 | MsG0380013968.01.T01:CDS | 35.0% | |
| GTTATAAATTGGGGGTTATC+AGG | + | Chr3:48532615-48532634 | MsG0380013968.01.T01:CDS | 35.0% | |
| TAATCCCAACGTGTTACATA+AGG | - | Chr3:48532171-48532190 | None:intergenic | 35.0% | |
| TCTTCGACTTATTGTTACTC+TGG | + | Chr3:48532050-48532069 | MsG0380013968.01.T01:CDS | 35.0% | |
| TGCAAATTGATAAAACCAGG+TGG | - | Chr3:48533044-48533063 | None:intergenic | 35.0% | |
| TGTTTGGGCAATTCAAAAGT+CGG | - | Chr3:48532927-48532946 | None:intergenic | 35.0% | |
| ! | AAATTTTCATATGTCCCACC+TGG | - | Chr3:48532825-48532844 | None:intergenic | 35.0% |
| ! | CAGAGGTATGGAAAAAATTG+CGG | + | Chr3:48532119-48532138 | MsG0380013968.01.T01:CDS | 35.0% |
| ! | GCTCGTTATTTAGTTCGAAT+TGG | + | Chr3:48532750-48532769 | MsG0380013968.01.T01:CDS | 35.0% |
| !! | ATTATTCAACAGGCTTTGGA+AGG | + | Chr3:48532795-48532814 | MsG0380013968.01.T01:CDS | 35.0% |
| AAATTCCTAATTCCCCCTTG+GGG | - | Chr3:48532974-48532993 | None:intergenic | 40.0% | |
| ACGTAGATCCCATTGTATTC+CGG | - | Chr3:48532658-48532677 | None:intergenic | 40.0% | |
| AGCAATAACCGTAAATGGAC+CGG | + | Chr3:48532212-48532231 | MsG0380013968.01.T01:CDS | 40.0% | |
| ATAGAGAAAAGGAGCCAGAA+TGG | + | Chr3:48532862-48532881 | MsG0380013968.01.T01:CDS | 40.0% | |
| ATCTGCCTTATGTAACACGT+TGG | + | Chr3:48532163-48532182 | MsG0380013968.01.T01:CDS | 40.0% | |
| CAATTGTTCCGGTCCATTTA+CGG | - | Chr3:48532223-48532242 | None:intergenic | 40.0% | |
| CGACTTATTGTTACTCTGGA+TGG | + | Chr3:48532054-48532073 | MsG0380013968.01.T01:CDS | 40.0% | |
| GCACCAATATCTGCCATAAA+AGG | - | Chr3:48532336-48532355 | None:intergenic | 40.0% | |
| GCATAGCTTCTCATCTACTA+TGG | + | Chr3:48532304-48532323 | MsG0380013968.01.T01:CDS | 40.0% | |
| GGGTTATTTACACAGAGGTA+TGG | + | Chr3:48532107-48532126 | MsG0380013968.01.T01:CDS | 40.0% | |
| GTAAATGGACCGGAACAATT+GGG | + | Chr3:48532222-48532241 | MsG0380013968.01.T01:CDS | 40.0% | |
| TCGCTCCTTTGATAGAGAAA+AGG | + | Chr3:48532851-48532870 | MsG0380013968.01.T01:CDS | 40.0% | |
| TCTGCCTTATGTAACACGTT+GGG | + | Chr3:48532164-48532183 | MsG0380013968.01.T01:CDS | 40.0% | |
| ! | AGAATGGTTTTCCTTGGAGA+TGG | + | Chr3:48533009-48533028 | MsG0380013968.01.T01:CDS | 40.0% |
| ! | GAAGAACACCATGCATTGAT+GGG | - | Chr3:48532035-48532054 | None:intergenic | 40.0% |
| ! | GAGATCAGAATGGTTTTCCT+TGG | + | Chr3:48533003-48533022 | MsG0380013968.01.T01:CDS | 40.0% |
| ! | GGACCTTTTATGGCAGATAT+TGG | + | Chr3:48532330-48532349 | MsG0380013968.01.T01:CDS | 40.0% |
| ! | TGGCTCCTTTTCTCTATCAA+AGG | - | Chr3:48532859-48532878 | None:intergenic | 40.0% |
| ! | TTTTCGTATTGGAGGAGTAG+CGG | + | Chr3:48532446-48532465 | MsG0380013968.01.T01:CDS | 40.0% |
| !! | CGTATATAGCTGGCTCTTTT+AGG | - | Chr3:48532261-48532280 | None:intergenic | 40.0% |
| AATTCCTAATTCCCCCTTGG+GGG | - | Chr3:48532973-48532992 | None:intergenic | 45.0% | |
| AGATGGAAAATTCGTCCACC+TGG | + | Chr3:48533026-48533045 | MsG0380013968.01.T01:CDS | 45.0% | |
| AGCTTCTCATCTACTATGGC+TGG | + | Chr3:48532308-48532327 | MsG0380013968.01.T01:CDS | 45.0% | |
| CGAAGAACACCATGCATTGA+TGG | - | Chr3:48532036-48532055 | None:intergenic | 45.0% | |
| CGTAAATGGACCGGAACAAT+TGG | + | Chr3:48532221-48532240 | MsG0380013968.01.T01:CDS | 45.0% | |
| GCTTCTCATCTACTATGGCT+GGG | + | Chr3:48532309-48532328 | MsG0380013968.01.T01:CDS | 45.0% | |
| ! | ACTATGGCTGGGACCTTTTA+TGG | + | Chr3:48532320-48532339 | MsG0380013968.01.T01:CDS | 45.0% |
| ! | CTTTGGAAGGAATTCCAGGT+GGG | + | Chr3:48532808-48532827 | MsG0380013968.01.T01:CDS | 45.0% |
| ! | GATCTATTTGAAGCTGCGAC+AGG | + | Chr3:48532402-48532421 | MsG0380013968.01.T01:CDS | 45.0% |
| ! | GGACGAATTTTCCATCTCCA+AGG | - | Chr3:48533023-48533042 | None:intergenic | 45.0% |
| ! | TTAGAACGAGTTGAAGGCGT+AGG | + | Chr3:48532573-48532592 | MsG0380013968.01.T01:CDS | 45.0% |
| !! | TTGAAGGCGTAGGTGTTGTT+GGG | + | Chr3:48532583-48532602 | MsG0380013968.01.T01:CDS | 45.0% |
| CCTCAACACCCATCAATGCA+TGG | + | Chr3:48532024-48532043 | MsG0380013968.01.T01:CDS | 50.0% | |
| CGGCTGATCTACCTTATGGT+TGG | + | Chr3:48532466-48532485 | MsG0380013968.01.T01:CDS | 50.0% | |
| GGTTATCAGGACCGATGCTT+CGG | + | Chr3:48532628-48532647 | MsG0380013968.01.T01:CDS | 50.0% | |
| GTAGCGGCTGATCTACCTTA+TGG | + | Chr3:48532462-48532481 | MsG0380013968.01.T01:CDS | 50.0% | |
| GTTATCAGGACCGATGCTTC+GGG | + | Chr3:48532629-48532648 | MsG0380013968.01.T01:CDS | 50.0% | |
| TCGGGCTTCCGGAATACAAT+GGG | + | Chr3:48532647-48532666 | MsG0380013968.01.T01:CDS | 50.0% | |
| TTCGGGCTTCCGGAATACAA+TGG | + | Chr3:48532646-48532665 | MsG0380013968.01.T01:CDS | 50.0% | |
| ! | CCATGCATTGATGGGTGTTG+AGG | - | Chr3:48532027-48532046 | None:intergenic | 50.0% |
| ! | GTTGAAGGCGTAGGTGTTGT+TGG | + | Chr3:48532582-48532601 | MsG0380013968.01.T01:CDS | 50.0% |
| !! | CAGGCTTTGGAAGGAATTCC+AGG | + | Chr3:48532804-48532823 | MsG0380013968.01.T01:CDS | 50.0% |
| !! | GCTTTGGAAGGAATTCCAGG+TGG | + | Chr3:48532807-48532826 | MsG0380013968.01.T01:CDS | 50.0% |
| ATTCCGGAAGCCCGAAGCAT+CGG | - | Chr3:48532642-48532661 | None:intergenic | 55.0% | |
| GTGAGAGTTGAGGCCCCCAA+GGG | + | Chr3:48532957-48532976 | MsG0380013968.01.T01:CDS | 60.0% | |
| TGAGAGTTGAGGCCCCCAAG+GGG | + | Chr3:48532958-48532977 | MsG0380013968.01.T01:CDS | 60.0% | |
| TGTGAGAGTTGAGGCCCCCA+AGG | + | Chr3:48532956-48532975 | MsG0380013968.01.T01:CDS | 60.0% | |
| GAGAGTTGAGGCCCCCAAGG+GGG | + | Chr3:48532959-48532978 | MsG0380013968.01.T01:CDS | 65.0% | |
| GAGGCCCCCAAGGGGGAATT+AGG | + | Chr3:48532966-48532985 | MsG0380013968.01.T01:CDS | 65.0% | |
| GGACCGATGCTTCGGGCTTC+CGG | + | Chr3:48532636-48532655 | MsG0380013968.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48532018 | 48533157 | 48532018 | ID=MsG0380013968.01;Name=MsG0380013968.01 |
| Chr3 | mRNA | 48532018 | 48533157 | 48532018 | ID=MsG0380013968.01.T01;Parent=MsG0380013968.01;Name=MsG0380013968.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|379 |
| Chr3 | exon | 48532018 | 48533157 | 48532018 | ID=MsG0380013968.01.T01:exon:8513;Parent=MsG0380013968.01.T01 |
| Chr3 | CDS | 48532018 | 48533157 | 48532018 | ID=MsG0380013968.01.T01:cds;Parent=MsG0380013968.01.T01 |
| Gene Sequence |
| Protein sequence |
