Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014039.01.T01 | YP_001381693.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 3.95E-81 | 243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014039.01.T01 | B5LMQ9 | 95.082 | 122 | 6 | 0 | 1 | 122 | 1 | 122 | 1.25E-80 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014039.01.T01 | A0A0K0LU84 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.89e-81 | 243 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0380014039.01 | -0.805551 | 1.264061e-49 | 6.706745e-47 |
| MsG0080048690.01 | MsG0380014039.01 | 0.825744 | 3.967529e-54 | 3.648127e-51 |
| MsG0080048692.01 | MsG0380014039.01 | 0.899377 | 2.105485e-77 | 2.596724e-73 |
| MsG0080048693.01 | MsG0380014039.01 | 0.835977 | 1.225316e-56 | 1.522875e-53 |
| MsG0080048698.01 | MsG0380014039.01 | 0.903476 | 3.336448e-79 | 4.947776e-75 |
| MsG0080048699.01 | MsG0380014039.01 | 0.943463 | 1.150968e-102 | 1.552499e-97 |
| MsG0080049076.01 | MsG0380014039.01 | 0.826820 | 2.199389e-54 | 2.085287e-51 |
| MsG0180002226.01 | MsG0380014039.01 | 0.808555 | 2.923412e-50 | 1.676665e-47 |
| MsG0180003624.01 | MsG0380014039.01 | 0.817104 | 3.920132e-52 | 2.828804e-49 |
| MsG0180004435.01 | MsG0380014039.01 | 0.816617 | 5.042962e-52 | 3.590837e-49 |
| MsG0280010695.01 | MsG0380014039.01 | 0.820792 | 5.688332e-53 | 4.545286e-50 |
| MsG0380013448.01 | MsG0380014039.01 | 0.849328 | 3.470697e-60 | 6.556921e-57 |
| MsG0380013888.01 | MsG0380014039.01 | 0.861350 | 1.091478e-63 | 3.079304e-60 |
| MsG0380013916.01 | MsG0380014039.01 | 0.868154 | 8.059384e-66 | 2.886019e-62 |
| MsG0380013917.01 | MsG0380014039.01 | 0.881443 | 2.391191e-70 | 1.401317e-66 |
| MsG0380013918.01 | MsG0380014039.01 | 0.806171 | 9.364280e-50 | 5.048325e-47 |
| MsG0380013920.01 | MsG0380014039.01 | 0.872824 | 2.362713e-67 | 1.002683e-63 |
| MsG0380013923.01 | MsG0380014039.01 | 0.964920 | 6.137597e-124 | 3.759016e-118 |
| MsG0380013926.01 | MsG0380014039.01 | 0.891627 | 3.341822e-74 | 2.953309e-70 |
| MsG0380013929.01 | MsG0380014039.01 | 0.885489 | 7.780936e-72 | 5.350963e-68 |
| MsG0380013931.01 | MsG0380014039.01 | 0.900124 | 1.002000e-77 | 1.277546e-73 |
| MsG0380013932.01 | MsG0380014039.01 | 0.841730 | 3.979810e-58 | 5.900754e-55 |
| MsG0380013940.01 | MsG0380014039.01 | 0.853326 | 2.572370e-61 | 5.537685e-58 |
| MsG0380013941.01 | MsG0380014039.01 | 0.903835 | 2.298756e-79 | 3.460721e-75 |
| MsG0380013942.01 | MsG0380014039.01 | 0.938953 | 2.862948e-99 | 2.852992e-94 |
| MsG0380013943.01 | MsG0380014039.01 | 0.853773 | 1.914571e-61 | 4.183013e-58 |
| MsG0380013944.01 | MsG0380014039.01 | 0.967648 | 1.440614e-127 | 1.111656e-121 |
| MsG0380013945.01 | MsG0380014039.01 | 0.856358 | 3.396605e-62 | 8.085840e-59 |
| MsG0380013948.01 | MsG0380014039.01 | 0.863798 | 1.924213e-64 | 5.907765e-61 |
| MsG0380013950.01 | MsG0380014039.01 | 0.801128 | 1.042026e-48 | 4.937797e-46 |
| MsG0380013951.01 | MsG0380014039.01 | 0.954173 | 5.357503e-112 | 1.482368e-106 |
| MsG0380013952.01 | MsG0380014039.01 | 0.904047 | 1.844312e-79 | 2.802258e-75 |
| MsG0380013953.01 | MsG0380014039.01 | 0.848718 | 5.127353e-60 | 9.495819e-57 |
| MsG0380013957.01 | MsG0380014039.01 | 0.852590 | 4.176714e-61 | 8.769594e-58 |
| MsG0380013959.01 | MsG0380014039.01 | 0.825821 | 3.803420e-54 | 3.504842e-51 |
| MsG0380013961.01 | MsG0380014039.01 | 0.845196 | 4.722055e-59 | 7.808099e-56 |
| MsG0380013962.01 | MsG0380014039.01 | 0.846257 | 2.432687e-59 | 4.162533e-56 |
| MsG0380013966.01 | MsG0380014039.01 | 0.929551 | 5.908447e-93 | 3.321803e-88 |
| MsG0380013967.01 | MsG0380014039.01 | 0.853523 | 2.259543e-61 | 4.896231e-58 |
| MsG0380013968.01 | MsG0380014039.01 | 0.952530 | 1.986941e-110 | 4.895894e-105 |
| MsG0380013969.01 | MsG0380014039.01 | 0.906222 | 1.868363e-80 | 3.132573e-76 |
| MsG0380013971.01 | MsG0380014039.01 | 0.834002 | 3.854382e-56 | 4.514865e-53 |
| MsG0380013972.01 | MsG0380014039.01 | 0.901705 | 2.043908e-78 | 2.795166e-74 |
| MsG0380013977.01 | MsG0380014039.01 | 0.855428 | 6.350325e-62 | 1.465708e-58 |
| MsG0380013981.01 | MsG0380014039.01 | 0.887983 | 8.833055e-73 | 6.717326e-69 |
| MsG0380013995.01 | MsG0380014039.01 | 0.922817 | 6.001651e-89 | 2.320341e-84 |
| MsG0380014004.01 | MsG0380014039.01 | 0.826905 | 2.098785e-54 | 1.994983e-51 |
| MsG0380014005.01 | MsG0380014039.01 | 0.857765 | 1.306157e-62 | 3.259173e-59 |
| MsG0380014006.01 | MsG0380014039.01 | 0.851526 | 8.378241e-61 | 1.700622e-57 |
| MsG0380014008.01 | MsG0380014039.01 | 0.945079 | 5.966440e-104 | 8.916575e-99 |
| MsG0380014011.01 | MsG0380014039.01 | 0.820197 | 7.788913e-53 | 6.122308e-50 |
| MsG0380014018.01 | MsG0380014039.01 | 0.923914 | 1.415704e-89 | 5.820239e-85 |
| MsG0380014020.01 | MsG0380014039.01 | 0.807265 | 5.500675e-50 | 3.050082e-47 |
| MsG0380014023.01 | MsG0380014039.01 | 0.890117 | 1.316598e-73 | 1.092469e-69 |
| MsG0380014026.01 | MsG0380014039.01 | 0.900361 | 7.908217e-78 | 1.019634e-73 |
| MsG0380014032.01 | MsG0380014039.01 | 0.856567 | 2.948872e-62 | 7.068893e-59 |
| MsG0380014033.01 | MsG0380014039.01 | 0.899776 | 1.417092e-77 | 1.777680e-73 |
| MsG0380014034.01 | MsG0380014039.01 | 0.820362 | 7.139326e-53 | 5.637049e-50 |
| MsG0380014036.01 | MsG0380014039.01 | 0.853039 | 3.109071e-61 | 6.628111e-58 |
| MsG0380014039.01 | MsG0380014040.01 | 0.964907 | 6.370825e-124 | 3.891340e-118 |
| MsG0380014039.01 | MsG0380014044.01 | 0.853479 | 2.326373e-61 | 5.033393e-58 |
| MsG0380014039.01 | MsG0380014051.01 | 0.910862 | 1.164542e-82 | 2.427076e-78 |
| MsG0380014039.01 | MsG0380014054.01 | 0.811434 | 7.014686e-51 | 4.341768e-48 |
| MsG0380014039.01 | MsG0380014057.01 | 0.916159 | 2.489322e-85 | 6.751414e-81 |
| MsG0380014039.01 | MsG0380017007.01 | 0.947235 | 9.973115e-106 | 1.723436e-100 |
| MsG0380014039.01 | MsG0480018296.01 | 0.894612 | 2.093314e-75 | 2.096576e-71 |
| MsG0380014039.01 | MsG0480020052.01 | 0.824039 | 1.001770e-53 | 8.772437e-51 |
| MsG0380014039.01 | MsG0480023810.01 | -0.828244 | 1.001247e-54 | 9.895754e-52 |
| MsG0380014039.01 | MsG0580024570.01 | 0.817733 | 2.829932e-52 | 2.077669e-49 |
| MsG0380014039.01 | MsG0580027677.01 | 0.844462 | 7.447974e-59 | 1.203270e-55 |
| MsG0380014039.01 | MsG0580029583.01 | -0.809988 | 1.441350e-50 | 8.586776e-48 |
| MsG0380014039.01 | MsG0780035951.01 | 0.822852 | 1.897339e-53 | 1.606939e-50 |
| MsG0380014039.01 | MsG0780038197.01 | 0.821008 | 5.072556e-53 | 4.078236e-50 |
| MsG0380014039.01 | MsG0780040316.01 | 0.820802 | 5.658067e-53 | 4.522432e-50 |
| MsG0380014039.01 | MsG0880045630.01 | 0.826079 | 3.302584e-54 | 3.065634e-51 |
| MsG0280006475.01 | MsG0380014039.01 | 0.801163 | 1.024901e-48 | 4.860847e-46 |
| MsG0280009971.01 | MsG0380014039.01 | -0.846676 | 1.869579e-59 | 3.243210e-56 |
| MsG0280010140.01 | MsG0380014039.01 | 0.822612 | 2.158253e-53 | 1.815671e-50 |
| MsG0280010145.01 | MsG0380014039.01 | 0.935023 | 1.624497e-96 | 1.277180e-91 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014039.01.T01 | MTR_4g051390 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 4.79e-85 | 243 |
| MsG0380014039.01.T01 | MTR_1779s0020 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.33e-78 | 227 |
| MsG0380014039.01.T01 | MTR_2g087550 | 91.803 | 122 | 10 | 0 | 1 | 122 | 1 | 122 | 1.82e-76 | 225 |
| MsG0380014039.01.T01 | MTR_0002s0780 | 77.679 | 112 | 24 | 1 | 12 | 122 | 139 | 250 | 5.62e-57 | 177 |
| MsG0380014039.01.T01 | MTR_5g096490 | 42.975 | 121 | 67 | 1 | 1 | 121 | 14 | 132 | 8.63e-27 | 97.1 |
| MsG0380014039.01.T01 | MTR_5g007240 | 42.975 | 121 | 67 | 1 | 1 | 121 | 47 | 165 | 1.88e-26 | 97.1 |
| MsG0380014039.01.T01 | MTR_5g096490 | 42.975 | 121 | 67 | 1 | 1 | 121 | 47 | 165 | 1.88e-26 | 97.1 |
| MsG0380014039.01.T01 | MTR_7g098290 | 37.391 | 115 | 64 | 3 | 8 | 118 | 11 | 121 | 3.00e-16 | 69.7 |
| MsG0380014039.01.T01 | MTR_7g098290 | 37.391 | 115 | 64 | 3 | 8 | 118 | 26 | 136 | 5.62e-16 | 69.3 |
| MsG0380014039.01.T01 | MTR_1g069585 | 37.391 | 115 | 64 | 3 | 8 | 118 | 26 | 136 | 5.62e-16 | 69.3 |
| MsG0380014039.01.T01 | MTR_4g116740 | 85.714 | 35 | 5 | 0 | 65 | 99 | 65 | 99 | 2.39e-14 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014039.01.T01 | ATCG00780 | 87.705 | 122 | 15 | 0 | 1 | 122 | 1 | 122 | 1.95e-77 | 224 |
| MsG0380014039.01.T01 | AT5G46160 | 39.062 | 128 | 69 | 3 | 1 | 121 | 46 | 171 | 2.97e-25 | 94.0 |
| MsG0380014039.01.T01 | AT5G46160 | 39.062 | 128 | 69 | 3 | 1 | 121 | 47 | 172 | 3.21e-25 | 94.0 |
| MsG0380014039.01.T01 | AT1G17560 | 30.464 | 151 | 74 | 4 | 1 | 122 | 25 | 173 | 2.54e-16 | 71.2 |
| MsG0380014039.01.T01 | AT1G17560 | 30.464 | 151 | 74 | 4 | 1 | 122 | 48 | 196 | 3.07e-16 | 71.2 |
| MsG0380014039.01.T01 | AT3G04400 | 36.522 | 115 | 65 | 3 | 8 | 118 | 11 | 121 | 3.11e-16 | 69.7 |
| MsG0380014039.01.T01 | AT2G33370 | 36.522 | 115 | 65 | 3 | 8 | 118 | 11 | 121 | 3.11e-16 | 69.7 |
| MsG0380014039.01.T01 | AT3G04400 | 36.522 | 115 | 65 | 3 | 8 | 118 | 26 | 136 | 5.17e-16 | 69.7 |
| MsG0380014039.01.T01 | AT2G33370 | 36.522 | 115 | 65 | 3 | 8 | 118 | 26 | 136 | 5.17e-16 | 69.7 |
| MsG0380014039.01.T01 | AT1G04480 | 36.522 | 115 | 65 | 3 | 8 | 118 | 26 | 136 | 5.17e-16 | 69.7 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCTTTCTAAAGATGAATT+TGG | 0.231628 | 3:+48717476 | None:intergenic |
| ATTATCGGCTACATTTAAAT+AGG | 0.265072 | 3:+48717601 | None:intergenic |
| AATCGACGGTATGCTTATAT+TGG | 0.402113 | 3:-48717533 | MsG0380014039.01.T01:CDS |
| TAGAAAGATCTGAAGTGATC+AGG | 0.408019 | 3:-48717463 | MsG0380014039.01.T01:CDS |
| AATTTAACTGTCTCAATTCT+CGG | 0.413515 | 3:+48717309 | None:intergenic |
| TTGATGTGTATTCGAATCAT+AGG | 0.441171 | 3:-48717563 | MsG0380014039.01.T01:CDS |
| ATTTAACTGTCTCAATTCTC+GGG | 0.470566 | 3:+48717310 | None:intergenic |
| GCTACATTTAAATAGGTTTG+AGG | 0.470779 | 3:+48717608 | None:intergenic |
| TTCGCGGGCTCCGCTATTAT+CGG | 0.494194 | 3:+48717586 | None:intergenic |
| ATCTCGTATTATAATACCTC+GGG | 0.516039 | 3:+48717263 | None:intergenic |
| GATAAAGAAGGAAATCCAAA+AGG | 0.534245 | 3:-48717356 | MsG0380014039.01.T01:CDS |
| TCTCAATTCTCGGGCTATTG+CGG | 0.549036 | 3:+48717319 | None:intergenic |
| AGAAAGATCTGAAGTGATCA+GGG | 0.551046 | 3:-48717462 | MsG0380014039.01.T01:CDS |
| TTCGAATACACATCAATTCG+CGG | 0.564271 | 3:+48717570 | None:intergenic |
| GCGGTTCTCATTGATAAAGA+AGG | 0.571105 | 3:-48717368 | MsG0380014039.01.T01:CDS |
| AAAGAACTAAAACGTAGTAA+TGG | 0.588124 | 3:-48717419 | MsG0380014039.01.T01:CDS |
| TCGAATACACATCAATTCGC+GGG | 0.590291 | 3:+48717571 | None:intergenic |
| AATAGTTTCATTAGCACCCG+AGG | 0.609986 | 3:-48717279 | MsG0380014039.01.T01:CDS |
| GATCTCGTATTATAATACCT+CGG | 0.645272 | 3:+48717262 | None:intergenic |
| AATAAAGTATGATGATAATG+CGG | 0.648055 | 3:-48717390 | MsG0380014039.01.T01:CDS |
| TTAAATGTAGCCGATAATAG+CGG | 0.666581 | 3:-48717596 | MsG0380014039.01.T01:CDS |
| TCATAGGAGCTAGTAATCGA+CGG | 0.670576 | 3:-48717547 | MsG0380014039.01.T01:CDS |
| AAAGTATGATGATAATGCGG+CGG | 0.676057 | 3:-48717387 | MsG0380014039.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAAGTATGATGATAATG+CGG | - | Chr3:48717498-48717517 | MsG0380014039.01.T01:CDS | 20.0% |
| ! | AAAGAACTAAAACGTAGTAA+TGG | - | Chr3:48717469-48717488 | MsG0380014039.01.T01:CDS | 25.0% |
| ! | AATTTAACTGTCTCAATTCT+CGG | + | Chr3:48717582-48717601 | None:intergenic | 25.0% |
| !! | ATTATCGGCTACATTTAAAT+AGG | + | Chr3:48717290-48717309 | None:intergenic | 25.0% |
| !! | GATCTTTCTAAAGATGAATT+TGG | + | Chr3:48717415-48717434 | None:intergenic | 25.0% |
| ATTTAACTGTCTCAATTCTC+GGG | + | Chr3:48717581-48717600 | None:intergenic | 30.0% | |
| GATAAAGAAGGAAATCCAAA+AGG | - | Chr3:48717532-48717551 | MsG0380014039.01.T01:CDS | 30.0% | |
| GCTACATTTAAATAGGTTTG+AGG | + | Chr3:48717283-48717302 | None:intergenic | 30.0% | |
| TTAAATGTAGCCGATAATAG+CGG | - | Chr3:48717292-48717311 | MsG0380014039.01.T01:CDS | 30.0% | |
| TTGATGTGTATTCGAATCAT+AGG | - | Chr3:48717325-48717344 | MsG0380014039.01.T01:CDS | 30.0% | |
| !! | GAAAAAATTCGAGTTCCTTT+TGG | + | Chr3:48717550-48717569 | None:intergenic | 30.0% |
| AATCGACGGTATGCTTATAT+TGG | - | Chr3:48717355-48717374 | MsG0380014039.01.T01:CDS | 35.0% | |
| TTCGAATACACATCAATTCG+CGG | + | Chr3:48717321-48717340 | None:intergenic | 35.0% | |
| ! | AAAGTATGATGATAATGCGG+CGG | - | Chr3:48717501-48717520 | MsG0380014039.01.T01:CDS | 35.0% |
| ! | AGAAAGATCTGAAGTGATCA+GGG | - | Chr3:48717426-48717445 | MsG0380014039.01.T01:CDS | 35.0% |
| ! | TAGAAAGATCTGAAGTGATC+AGG | - | Chr3:48717425-48717444 | MsG0380014039.01.T01:CDS | 35.0% |
| AATAGTTTCATTAGCACCCG+AGG | - | Chr3:48717609-48717628 | MsG0380014039.01.T01:CDS | 40.0% | |
| GCGGTTCTCATTGATAAAGA+AGG | - | Chr3:48717520-48717539 | MsG0380014039.01.T01:CDS | 40.0% | |
| TCATAGGAGCTAGTAATCGA+CGG | - | Chr3:48717341-48717360 | MsG0380014039.01.T01:CDS | 40.0% | |
| TCGAATACACATCAATTCGC+GGG | + | Chr3:48717320-48717339 | None:intergenic | 40.0% | |
| TCTCAATTCTCGGGCTATTG+CGG | + | Chr3:48717572-48717591 | None:intergenic | 45.0% | |
| TTCGCGGGCTCCGCTATTAT+CGG | + | Chr3:48717305-48717324 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48717271 | 48717639 | 48717271 | ID=MsG0380014039.01;Name=MsG0380014039.01 |
| Chr3 | mRNA | 48717271 | 48717639 | 48717271 | ID=MsG0380014039.01.T01;Parent=MsG0380014039.01;Name=MsG0380014039.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|122 |
| Chr3 | exon | 48717271 | 48717639 | 48717271 | ID=MsG0380014039.01.T01:exon:56992;Parent=MsG0380014039.01.T01 |
| Chr3 | CDS | 48717271 | 48717639 | 48717271 | ID=MsG0380014039.01.T01:cds;Parent=MsG0380014039.01.T01 |
| Gene Sequence |
| Protein sequence |
