AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014018.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014018.01.T01 MTR_1829s0010 97.015 67 2 0 1 67 51 117 5.66e-40 134
MsG0380014018.01.T01 MTR_4g451275 98.507 67 1 0 1 67 306 372 3.80e-39 134
MsG0380014018.01.T01 MTR_7g033765 98.507 67 1 0 1 67 306 372 3.94e-39 135
MsG0380014018.01.T01 MTR_3g035730 98.507 67 1 0 1 67 306 372 4.21e-39 134
MsG0380014018.01.T01 MTR_7g021780 98.507 67 1 0 1 67 306 372 5.41e-39 135
MsG0380014018.01.T01 MTR_0002s0650 94.030 67 4 0 1 67 306 372 5.56e-37 129
MsG0380014018.01.T01 MTR_1g076100 85.075 67 7 1 1 67 1 64 4.82e-33 108
MsG0380014018.01.T01 MTR_1g108765 82.090 67 12 0 1 67 366 432 2.50e-32 116
MsG0380014018.01.T01 MTR_1g108770 82.090 67 12 0 1 67 369 435 2.90e-32 116
MsG0380014018.01.T01 MTR_7g033755 77.778 54 12 0 14 67 218 271 3.39e-21 85.1
MsG0380014018.01.T01 MTR_2g066870 46.154 65 26 2 3 67 240 295 3.52e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014018.01.T01 ATCG00480 95.522 67 3 0 1 67 306 372 4.32e-38 131
MsG0380014018.01.T01 AT5G08690 82.090 67 12 0 1 67 366 432 2.77e-32 116
MsG0380014018.01.T01 AT5G08670 82.090 67 12 0 1 67 366 432 2.94e-32 116
MsG0380014018.01.T01 AT5G08680 82.090 67 12 0 1 67 369 435 2.98e-32 116

Find 15 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCACGACATTTGCACATT+TGG 0.255396 3:-48664115 MsG0380014018.01.T01:CDS
AGAAGTTATAGACCCTTCTT+TGG 0.362123 3:+48664185 None:intergenic
CGAATTACTTCTACCAAAGA+AGG 0.431473 3:-48664198 MsG0380014018.01.T01:CDS
GACTGCTGGATAGATACCTT+TGG 0.462417 3:+48664059 None:intergenic
GATCCTTTAGATTCGACTGC+TGG 0.477320 3:-48664036 MsG0380014018.01.T01:CDS
GCAAATGTCGTGGCAGGAGC+AGG 0.482962 3:+48664123 None:intergenic
GAATCTAAAGGATCGACTGC+TGG 0.483621 3:+48664045 None:intergenic
AAATGTGCAAATGTCGTGGC+AGG 0.534291 3:+48664117 None:intergenic
TATCCAGCAGTCGAATCTAA+AGG 0.541007 3:+48664033 None:intergenic
GGATCGGTCAAATCGTCTGC+AGG 0.560812 3:+48664144 None:intergenic
TGTCGTGGCAGGAGCAGGAT+CGG 0.567561 3:+48664128 None:intergenic
GCTACTACTGTACTATCAAG+AGG 0.609593 3:-48664090 MsG0380014018.01.T01:CDS
TCAAGAGGATTAGCTTCCAA+AGG 0.627287 3:-48664075 MsG0380014018.01.T01:CDS
ATCCAAATGTGCAAATGTCG+TGG 0.630761 3:+48664113 None:intergenic
GAATTACTTCTACCAAAGAA+GGG 0.652866 3:-48664197 MsG0380014018.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAATTACTTCTACCAAAGAA+GGG - Chr3:48664051-48664070 MsG0380014018.01.T01:CDS 30.0%
AGAAGTTATAGACCCTTCTT+TGG + Chr3:48664066-48664085 None:intergenic 35.0%
CGAATTACTTCTACCAAAGA+AGG - Chr3:48664050-48664069 MsG0380014018.01.T01:CDS 35.0%
ATCCAAATGTGCAAATGTCG+TGG + Chr3:48664138-48664157 None:intergenic 40.0%
GCTACTACTGTACTATCAAG+AGG - Chr3:48664158-48664177 MsG0380014018.01.T01:CDS 40.0%
TATCCAGCAGTCGAATCTAA+AGG + Chr3:48664218-48664237 None:intergenic 40.0%
!! TCAAGAGGATTAGCTTCCAA+AGG - Chr3:48664173-48664192 MsG0380014018.01.T01:CDS 40.0%
AAATGTGCAAATGTCGTGGC+AGG + Chr3:48664134-48664153 None:intergenic 45.0%
GAATCTAAAGGATCGACTGC+TGG + Chr3:48664206-48664225 None:intergenic 45.0%
GACTGCTGGATAGATACCTT+TGG + Chr3:48664192-48664211 None:intergenic 45.0%
GATCCTTTAGATTCGACTGC+TGG - Chr3:48664212-48664231 MsG0380014018.01.T01:CDS 45.0%
TGCCACGACATTTGCACATT+TGG - Chr3:48664133-48664152 MsG0380014018.01.T01:CDS 45.0%
GGATCGGTCAAATCGTCTGC+AGG + Chr3:48664107-48664126 None:intergenic 55.0%
GCAAATGTCGTGGCAGGAGC+AGG + Chr3:48664128-48664147 None:intergenic 60.0%
TGTCGTGGCAGGAGCAGGAT+CGG + Chr3:48664123-48664142 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48664032 48664238 48664032 ID=MsG0380014018.01;Name=MsG0380014018.01
Chr3 mRNA 48664032 48664238 48664032 ID=MsG0380014018.01.T01;Parent=MsG0380014018.01;Name=MsG0380014018.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|68
Chr3 exon 48664032 48664238 48664032 ID=MsG0380014018.01.T01:exon:9254;Parent=MsG0380014018.01.T01
Chr3 CDS 48664032 48664238 48664032 ID=MsG0380014018.01.T01:cds;Parent=MsG0380014018.01.T01
Gene Sequence

>MsG0380014018.01.T01

ATGGGTACTTTACAAGAACGAATTACTTCTACCAAAGAAGGGTCTATAACTTCTATTCAAGCAGTTTATGTACCTGCAGACGATTTGACCGATCCTGCTCCTGCCACGACATTTGCACATTTGGATGCTACTACTGTACTATCAAGAGGATTAGCTTCCAAAGGTATCTATCCAGCAGTCGATCCTTTAGATTCGACTGCTGGATAG

Protein sequence

>MsG0380014018.01.T01

MGTLQERITSTKEGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLASKGIYPAVDPLDSTAG*