Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037148.01.T01 | XP_013447979.1 | 80.995 | 221 | 24 | 3 | 128 | 330 | 369 | 589 | 4.07E-111 | 345 |
| MsG0780037148.01.T01 | XP_013447979.1 | 94.643 | 56 | 3 | 0 | 1 | 56 | 58 | 113 | 4.44E-24 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037148.01.T01 | A0A072TXV1 | 80.995 | 221 | 24 | 3 | 128 | 330 | 369 | 589 | 1.95e-111 | 345 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048694.01 | MsG0780037148.01 | -0.804492 | 2.105275e-49 | 1.086955e-46 |
| MsG0080048695.01 | MsG0780037148.01 | -0.804805 | 1.810762e-49 | 9.425765e-47 |
| MsG0180005272.01 | MsG0780037148.01 | 0.812516 | 4.076293e-51 | 2.597198e-48 |
| MsG0180006191.01 | MsG0780037148.01 | 0.830747 | 2.466721e-55 | 2.622635e-52 |
| MsG0280007786.01 | MsG0780037148.01 | 0.800136 | 1.660025e-48 | 7.670598e-46 |
| MsG0280010240.01 | MsG0780037148.01 | 0.828446 | 8.949753e-55 | 8.898176e-52 |
| MsG0380013928.01 | MsG0780037148.01 | -0.804002 | 2.662186e-49 | 1.357380e-46 |
| MsG0380014029.01 | MsG0780037148.01 | -0.801030 | 1.091389e-48 | 5.159022e-46 |
| MsG0380014410.01 | MsG0780037148.01 | 0.812111 | 4.997683e-51 | 3.149598e-48 |
| MsG0380014642.01 | MsG0780037148.01 | -0.804802 | 1.813507e-49 | 9.439287e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037148.01.T01 | MTR_7g029015 | 80.995 | 221 | 24 | 3 | 128 | 330 | 369 | 589 | 4.93e-115 | 345 |
| MsG0780037148.01.T01 | MTR_7g029015 | 94.643 | 56 | 3 | 0 | 1 | 56 | 58 | 113 | 5.37e-28 | 114 |
| MsG0780037148.01.T01 | MTR_3g099150 | 42.009 | 219 | 103 | 5 | 128 | 323 | 342 | 559 | 9.76e-38 | 142 |
| MsG0780037148.01.T01 | MTR_3g099150 | 57.143 | 56 | 24 | 0 | 1 | 56 | 58 | 113 | 1.71e-13 | 71.6 |
| MsG0780037148.01.T01 | MTR_5g022380 | 37.037 | 162 | 84 | 1 | 165 | 326 | 277 | 420 | 5.80e-25 | 104 |
| MsG0780037148.01.T01 | MTR_4g073690 | 31.925 | 213 | 107 | 4 | 128 | 321 | 344 | 537 | 1.05e-24 | 104 |
| MsG0780037148.01.T01 | MTR_5g022380 | 37.037 | 162 | 84 | 1 | 165 | 326 | 387 | 530 | 1.11e-24 | 104 |
| MsG0780037148.01.T01 | MTR_2g100400 | 31.429 | 210 | 103 | 3 | 128 | 317 | 340 | 528 | 3.35e-23 | 100 |
| MsG0780037148.01.T01 | MTR_5g090570 | 31.579 | 171 | 91 | 3 | 162 | 330 | 386 | 532 | 6.52e-22 | 96.7 |
| MsG0780037148.01.T01 | MTR_1g111530 | 30.660 | 212 | 114 | 4 | 128 | 317 | 352 | 552 | 6.71e-21 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037148.01.T01 | AT5G14120 | 55.024 | 209 | 65 | 4 | 134 | 324 | 380 | 577 | 1.60e-65 | 216 |
| MsG0780037148.01.T01 | AT5G14120 | 75.000 | 56 | 14 | 0 | 1 | 56 | 59 | 114 | 5.44e-21 | 94.0 |
| MsG0780037148.01.T01 | AT3G01930 | 55.024 | 209 | 65 | 5 | 134 | 324 | 272 | 469 | 1.91e-65 | 214 |
| MsG0780037148.01.T01 | AT3G01930 | 55.024 | 209 | 65 | 5 | 134 | 324 | 385 | 582 | 3.33e-64 | 213 |
| MsG0780037148.01.T01 | AT3G01930 | 76.786 | 56 | 13 | 0 | 1 | 56 | 59 | 114 | 3.53e-21 | 94.7 |
| MsG0780037148.01.T01 | AT5G50520 | 39.352 | 216 | 100 | 5 | 128 | 325 | 336 | 538 | 3.18e-40 | 148 |
| MsG0780037148.01.T01 | AT5G50520 | 61.111 | 54 | 21 | 0 | 1 | 54 | 60 | 113 | 2.88e-14 | 73.9 |
| MsG0780037148.01.T01 | AT5G50630 | 39.352 | 216 | 100 | 5 | 128 | 325 | 336 | 538 | 3.18e-40 | 148 |
| MsG0780037148.01.T01 | AT5G50630 | 61.111 | 54 | 21 | 0 | 1 | 54 | 60 | 113 | 2.88e-14 | 73.9 |
| MsG0780037148.01.T01 | AT4G34950 | 30.455 | 220 | 115 | 4 | 128 | 328 | 361 | 561 | 1.39e-24 | 104 |
Find 68 sgRNAs with CRISPR-Local
Find 327 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACCTTGGTGATTGTGT+TGG | 0.574197 | 7:+20684621 | MsG0780037148.01.T01:CDS |
| AAATAATCAGAAGCTGTAGC+TGG | 0.498719 | 7:-20689045 | None:intergenic |
| AACATCTTGACACATCAATG+AGG | 0.707200 | 7:-20687253 | None:intergenic |
| AACTGTAAGAAATCAGTACT+AGG | 0.467490 | 7:-20687189 | None:intergenic |
| AAGTTCCTGTTCTTCCTTTA+TGG | 0.160040 | 7:+20684754 | MsG0780037148.01.T01:CDS |
| ACAAGTAAAGCTGCCCAAAT+AGG | 0.362216 | 7:-20684675 | None:intergenic |
| ACAGCCCATAAAGGAAGAAC+AGG | 0.480902 | 7:-20684759 | None:intergenic |
| ACTGTAAGAAATCAGTACTA+GGG | 0.527141 | 7:-20687188 | None:intergenic |
| ACTTGGTTACGGTGCTCATT+GGG | 0.463235 | 7:+20689014 | MsG0780037148.01.T01:CDS |
| AGAAGACAAGAGTTCCAACA+GGG | 0.668994 | 7:-20689116 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAACTTACAGCCCATAA+AGG | - | Chr7:20684771-20684790 | None:intergenic | 30.0% | |
| AACTGTAAGAAATCAGTACT+AGG | - | Chr7:20687192-20687211 | None:intergenic | 30.0% | |
| ACTGTAAGAAATCAGTACTA+GGG | - | Chr7:20687191-20687210 | None:intergenic | 30.0% | |
| ACTTTACTCAATTTGGTTGT+TGG | + | Chr7:20685867-20685886 | MsG0780037148.01.T01:intron | 30.0% | |
| ATACGAATGATGCTATATTC+AGG | - | Chr7:20689206-20689225 | None:intergenic | 30.0% | |
| ATGTGACATGATACTAATCT+TGG | + | Chr7:20685920-20685939 | MsG0780037148.01.T01:intron | 30.0% | |
| ATGTTACCATTATATCTGGA+GGG | - | Chr7:20685789-20685808 | None:intergenic | 30.0% | |
| CAACATGTTACCATTATATC+TGG | - | Chr7:20685793-20685812 | None:intergenic | 30.0% | |
| CACAATCCATGTTTATATTG+TGG | + | Chr7:20686888-20686907 | MsG0780037148.01.T01:intron | 30.0% | |
| CTCTAAATTTCGAGAAATCA+CGG | + | Chr7:20685105-20685124 | MsG0780037148.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 20684615 | 20689407 | 20684615 | ID=MsG0780037148.01;Name=MsG0780037148.01 |
| Chr7 | mRNA | 20684615 | 20689407 | 20684615 | ID=MsG0780037148.01.T01;Parent=MsG0780037148.01;Name=MsG0780037148.01.T01;_AED=0.38;_eAED=0.49;_QI=0|0|0|0.8|0|0|5|0|330 |
| Chr7 | exon | 20684615 | 20684779 | 20684615 | ID=MsG0780037148.01.T01:exon:5074;Parent=MsG0780037148.01.T01 |
| Chr7 | exon | 20687183 | 20687298 | 20687183 | ID=MsG0780037148.01.T01:exon:5075;Parent=MsG0780037148.01.T01 |
| Chr7 | exon | 20687411 | 20687511 | 20687411 | ID=MsG0780037148.01.T01:exon:5076;Parent=MsG0780037148.01.T01 |
| Chr7 | exon | 20688174 | 20688209 | 20688174 | ID=MsG0780037148.01.T01:exon:5077;Parent=MsG0780037148.01.T01 |
| Chr7 | exon | 20688833 | 20689407 | 20688833 | ID=MsG0780037148.01.T01:exon:5078;Parent=MsG0780037148.01.T01 |
| Chr7 | CDS | 20684615 | 20684779 | 20684615 | ID=MsG0780037148.01.T01:cds;Parent=MsG0780037148.01.T01 |
| Chr7 | CDS | 20687183 | 20687298 | 20687183 | ID=MsG0780037148.01.T01:cds;Parent=MsG0780037148.01.T01 |
| Chr7 | CDS | 20687411 | 20687511 | 20687411 | ID=MsG0780037148.01.T01:cds;Parent=MsG0780037148.01.T01 |
| Chr7 | CDS | 20688174 | 20688209 | 20688174 | ID=MsG0780037148.01.T01:cds;Parent=MsG0780037148.01.T01 |
| Chr7 | CDS | 20688833 | 20689407 | 20688833 | ID=MsG0780037148.01.T01:cds;Parent=MsG0780037148.01.T01 |
| Gene Sequence |
>MsG0780037148.01.T01 GTTGCAAAAGACCTTGGTGATTGTGTTGGTTTTTTAACTGGTGTTTTGTGTGAAATTTTACCTATTTGGGCAGCTTTACTTGTTGGAGCTTCTTTGAATCTTGTTGGTTATGGCTGGGTTTGGTTAATTGTTACTAGTAAAGTTCCTGTTCTTCCTTTATGGGCTGTTGCCCCTAGTACTGATTTCTTACAGTTGATGTCTCCTAAAGATCAAAAGTTAGTTAATATCCATCAGTCCTCATTGATGTGTCAAGATGTTGCACGTGTGTATCTGAGGACAATATGTGTATTCTTATATTTGTGGGAACAAATGGTGAAACCTACTTCAACACAGTTTCTTTGGTTTCTTGTGTTCAAAATTTCCGAAAAGCCGGGGTCCCGTGGTTCTGGATCTGGGTTGACCGTAATTGACAATCTCGGCGATAATCTTGTCAAAATTTCTAACATGGTTTTTTTAATTCCGATGTGTTTTACTGAGCAGGATCATACATATCCAAGACCAGCTGCATTAGCTGCATTTCAACTAGCTATGACTGTTGGTCATCTTTTCATTGGCATGGGATGGCCAGGTTCAATGTACGTTGGCACTTTACTAGTTGGACTTGGTTACGGTGCTCATTGGGCAGTTGTTCCAGCTACAGCTTCTGATTATTTTTGTTTAAGAAACTTTGGTGCATTATACAATTTCATCACTCTAGCAAACCCTGTTGGAACTCTTGTCTTCTCTAGTCTCATTGCTAGTAGGATCTATGATTATGAAGCAGAGAACCAAGCTCATGGTGGCCAAAACCTGAATATAGCATCATTCGTATCTAGTGCGCTTAATGTTGGTGACGCGCCAAAGTGCGAAGGTTCCATATGCTTCTTCCTGACATCCACTATCATGGCAGGATTATGCATTGTTGCGGCAGGGTTAAGCATGTTTCTCGTGTTCCGAACAAGGATTGTTTATGCTAGCTTGTATGGAAAATCTTCTATGCGAAGCCTTCGATGA |
| Protein sequence |
>MsG0780037148.01.T01 VAKDLGDCVGFLTGVLCEILPIWAALLVGASLNLVGYGWVWLIVTSKVPVLPLWAVAPSTDFLQLMSPKDQKLVNIHQSSLMCQDVARVYLRTICVFLYLWEQMVKPTSTQFLWFLVFKISEKPGSRGSGSGLTVIDNLGDNLVKISNMVFLIPMCFTEQDHTYPRPAALAAFQLAMTVGHLFIGMGWPGSMYVGTLLVGLGYGAHWAVVPATASDYFCLRNFGALYNFITLANPVGTLVFSSLIASRIYDYEAENQAHGGQNLNIASFVSSALNVGDAPKCEGSICFFLTSTIMAGLCIVAAGLSMFLVFRTRIVYASLYGKSSMRSLR* |
