Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038749.01.T01 | XP_024626395.1 | 98.058 | 412 | 3 | 1 | 28 | 439 | 1 | 407 | 0 | 833 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003590.01 | MsG0780038749.01 | 0.812936 | 3.298431e-51 | 2.124827e-48 |
| MsG0180005331.01 | MsG0780038749.01 | 0.803132 | 4.033973e-49 | 2.011204e-46 |
| MsG0280007848.01 | MsG0780038749.01 | 0.803303 | 3.717755e-49 | 1.861717e-46 |
| MsG0380015324.01 | MsG0780038749.01 | 0.806837 | 6.775226e-50 | 3.715346e-47 |
| MsG0380015931.01 | MsG0780038749.01 | 0.803108 | 4.080215e-49 | 2.032999e-46 |
| MsG0380017286.01 | MsG0780038749.01 | 0.825062 | 5.751052e-54 | 5.186320e-51 |
| MsG0480022274.01 | MsG0780038749.01 | 0.811939 | 5.446186e-51 | 3.416237e-48 |
| MsG0580027773.01 | MsG0780038749.01 | 0.817400 | 3.362937e-52 | 2.446557e-49 |
| MsG0680030720.01 | MsG0780038749.01 | 0.806583 | 7.667573e-50 | 4.177072e-47 |
| MsG0780038749.01 | MsG0880042368.01 | 0.806122 | 9.589914e-50 | 5.163431e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038749.01.T01 | MTR_7g061660 | 98.058 | 412 | 3 | 1 | 28 | 439 | 1 | 407 | 0.0 | 833 |
| MsG0780038749.01.T01 | MTR_2g096950 | 67.780 | 419 | 116 | 4 | 28 | 439 | 1 | 407 | 0.0 | 580 |
| MsG0780038749.01.T01 | MTR_5g079840 | 69.672 | 366 | 104 | 3 | 28 | 391 | 1 | 361 | 0.0 | 542 |
| MsG0780038749.01.T01 | MTR_3g067795 | 68.280 | 372 | 110 | 3 | 26 | 395 | 1 | 366 | 0.0 | 526 |
| MsG0780038749.01.T01 | MTR_3g067795 | 68.280 | 372 | 110 | 3 | 26 | 395 | 1 | 366 | 0.0 | 525 |
| MsG0780038749.01.T01 | MTR_4g063930 | 59.472 | 417 | 149 | 6 | 24 | 437 | 97 | 496 | 4.97e-173 | 494 |
| MsG0780038749.01.T01 | MTR_2g096970 | 64.560 | 364 | 110 | 4 | 28 | 390 | 1 | 346 | 8.90e-173 | 488 |
| MsG0780038749.01.T01 | MTR_5g038870 | 62.567 | 374 | 130 | 3 | 28 | 398 | 1 | 367 | 9.19e-171 | 485 |
| MsG0780038749.01.T01 | MTR_4g063945 | 57.042 | 426 | 150 | 6 | 24 | 436 | 5 | 410 | 5.27e-167 | 476 |
| MsG0780038749.01.T01 | MTR_4g063935 | 58.974 | 429 | 142 | 6 | 24 | 437 | 5 | 414 | 2.79e-166 | 474 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038749.01.T01 | AT5G02290 | 65.025 | 406 | 123 | 3 | 28 | 433 | 1 | 387 | 0.0 | 554 |
| MsG0780038749.01.T01 | AT5G02290 | 65.025 | 406 | 123 | 3 | 28 | 433 | 1 | 387 | 0.0 | 554 |
| MsG0780038749.01.T01 | AT2G28930 | 64.846 | 421 | 133 | 4 | 28 | 439 | 1 | 415 | 0.0 | 548 |
| MsG0780038749.01.T01 | AT2G28930 | 64.846 | 421 | 130 | 5 | 28 | 439 | 1 | 412 | 0.0 | 544 |
| MsG0780038749.01.T01 | AT1G07570 | 64.133 | 421 | 131 | 5 | 28 | 439 | 1 | 410 | 0.0 | 539 |
| MsG0780038749.01.T01 | AT1G07570 | 64.133 | 421 | 131 | 5 | 28 | 439 | 1 | 410 | 0.0 | 539 |
| MsG0780038749.01.T01 | AT1G07570 | 64.133 | 421 | 131 | 5 | 28 | 439 | 1 | 410 | 0.0 | 539 |
| MsG0780038749.01.T01 | AT2G28930 | 67.632 | 380 | 109 | 3 | 69 | 439 | 49 | 423 | 0.0 | 532 |
| MsG0780038749.01.T01 | AT1G07570 | 66.416 | 399 | 120 | 4 | 50 | 439 | 28 | 421 | 0.0 | 531 |
| MsG0780038749.01.T01 | AT2G39660 | 63.562 | 365 | 122 | 2 | 28 | 392 | 1 | 354 | 5.16e-170 | 483 |
Find 134 sgRNAs with CRISPR-Local
Find 331 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTCTGCGTTTGTTGGAT+AGG | 0.440029 | 7:-52437559 | None:intergenic |
| AAACTGTAGTGTTGTCTATG+TGG | 0.602569 | 7:+52438066 | MsG0780038749.01.T01:three_prime_UTR |
| AAATGCTAACCGGTGCCCCG+GGG | 0.714495 | 7:+52433629 | MsG0780038749.01.T01:CDS |
| AACCTCGGCCACAATGCATC+CGG | 0.455051 | 7:+52437779 | MsG0780038749.01.T01:CDS |
| AAGACATTGTGAAGCAAGGG+CGG | 0.635211 | 7:-52437642 | None:intergenic |
| AAGAGCGATGTATATAGTTT+TGG | 0.325980 | 7:+52437443 | MsG0780038749.01.T01:CDS |
| AAGGCTTGGCCCATTCGACT+AGG | 0.571185 | 7:-52437535 | None:intergenic |
| AAGTCACGGTATATGACTTT+AGG | 0.287225 | 7:-52436534 | None:intergenic |
| AATAAGGCCAAATATGGACG+AGG | 0.672932 | 7:+52437679 | MsG0780038749.01.T01:CDS |
| AATAATACCCATTCCTGGTT+TGG | 0.352579 | 7:-52435596 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACGTTAGTATTAGAACATAG+AGG | - | Chr7:52436395-52436414 | None:intergenic | 30.0% | |
| ATTAATAGGATACTGCTTTG+AGG | + | Chr7:52436212-52436231 | MsG0780038749.01.T01:CDS | 30.0% | |
| ATTCATGAAATGGGTACAAA+AGG | - | Chr7:52434240-52434259 | None:intergenic | 30.0% | |
| CAAGAATATTCCATAGATTC+AGG | - | Chr7:52434497-52434516 | None:intergenic | 30.0% | |
| CACAAAAAACTCAAACATGA+AGG | - | Chr7:52437865-52437884 | None:intergenic | 30.0% | |
| CATAGTTAATGAGAGAAAAC+TGG | - | Chr7:52436874-52436893 | None:intergenic | 30.0% | |
| CCTTAACTCAAAATGTTTGT+TGG | + | Chr7:52433970-52433989 | MsG0780038749.01.T01:intron | 30.0% | |
| GAAATCATCATCTTCAATAC+AGG | + | Chr7:52436420-52436439 | MsG0780038749.01.T01:intron | 30.0% | |
| GAGAAGATTTCATGAAGTTA+TGG | - | Chr7:52437278-52437297 | None:intergenic | 30.0% | |
| GTACGTATCATTTCTTCTTT+GGG | + | Chr7:52435673-52435692 | MsG0780038749.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 52433624 | 52438246 | 52433624 | ID=MsG0780038749.01;Name=MsG0780038749.01 |
| Chr7 | mRNA | 52433624 | 52438246 | 52433624 | ID=MsG0780038749.01.T01;Parent=MsG0780038749.01;Name=MsG0780038749.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0.66|0.57|0.85|0.83|0.85|7|372|439 |
| Chr7 | exon | 52433624 | 52433673 | 52433624 | ID=MsG0780038749.01.T01:exon:8315;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52434288 | 52434370 | 52434288 | ID=MsG0780038749.01.T01:exon:8316;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52435350 | 52435672 | 52435350 | ID=MsG0780038749.01.T01:exon:8317;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52436165 | 52436300 | 52436165 | ID=MsG0780038749.01.T01:exon:8318;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52436442 | 52436584 | 52436442 | ID=MsG0780038749.01.T01:exon:8319;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52436682 | 52436820 | 52436682 | ID=MsG0780038749.01.T01:exon:8320;Parent=MsG0780038749.01.T01 |
| Chr7 | exon | 52437429 | 52438246 | 52437429 | ID=MsG0780038749.01.T01:exon:8321;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52433624 | 52433673 | 52433624 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52434288 | 52434370 | 52434288 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52435350 | 52435672 | 52435350 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52436165 | 52436300 | 52436165 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52436442 | 52436584 | 52436442 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52436682 | 52436820 | 52436682 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | CDS | 52437429 | 52437874 | 52437429 | ID=MsG0780038749.01.T01:cds;Parent=MsG0780038749.01.T01 |
| Chr7 | three_prime_UTR | 52437875 | 52438246 | 52437875 | ID=MsG0780038749.01.T01:three_prime_utr;Parent=MsG0780038749.01.T01 |
| Gene Sequence |
>MsG0780038749.01.T01 ATGGGAAATGCTAACCGGTGCCCCGGGGCACTGGTTAAGGATATGAAAAAGCTTAAACTTTTATCAGTGATTTTGTTGTTGATGGGTGCTTGTTGGAGTAATAAGGTTAAGTCTGTGAGTCCTTCAAACACAGGGTTCACTTCAAGAAGTGTAAGCAGAAGTGGCCATGACATCAGCTCAACTAGCAGGAACTCATCAGCCTCTATATCAGTCACTTCTCGAAGTGAGGGTGAAATCTTGCAGTCTTCCAACTTGAAAAGCTTCAGCTACAATGAAGTCAGAGCGGCTACGAGAAATTTCCGGCCAGACAGTGTATTAGGGGAAGGTGGTTTTGGTTCAGTTTTTAAAGGCTGGATTGATGAGCATTCACATGCTGCAACCAAACCAGGAATGGGTATTATTGTTGCTGTGAAGAGGCTTAACCAAGATGGGCATCAGGGTCACAGAGAATGGTTGGCTGAAATCAACTATCTCGGGCAACTTCAGCATCCTAATCTTGTCAAATTAATAGGATACTGCTTTGAGGATGAACATCGGCTCCTCGTTTACGAGTTTATGCCAAAGGGAAGCATGGAAAATCATCTTTTTAGGCGAGGTTCTTATTTTCAACCATTCTCTTGGAGTTTGAGAATGAAAATCGCCCTCGGAGCTGCAAAAGGCCTTGCTTTTCTCCATAGTACAGAACCTAAAGTCATATACCGTGACTTCAAAACTTCGAATATACTGCTCGATTCTAACTATGATGCTAAACTTTCTGATTTTGGGTTGGCTAGAGATGGACCAACTGGAGATAAAAGCCATGTCTCTACTCGGGTCATGGGAACCCGTGGATATGCAGCACCAGAGTATCTAGCAACAGGTATTAACAGCTCCGGCCATCTTACTGCAAAGAGCGATGTATATAGTTTTGGAGTAGTTCTTCTAGAAATTATATCAGGAAGACGTGCCATAGACAAGAACCTGCCATCCGGAGAACACAACCTAGTCGAATGGGCCAAGCCTTACCTATCCAACAAACGCAGAGTTTTTCGTGTAATGGATCCACGACTCGAAGGTCAATATTCACACAGCCGAGCTCATGCTGCAGCCGCCCTTGCTTCACAATGTCTTTCTGTGGAACCCAGAATAAGGCCAAATATGGACGAGGTTGTTAAAACATTGGAGCAGCTTCAGGAACCAAAGGACGCTCAGAAGAAAGGTTCTGATCACCGCGCACGCAATTCCAACCTCGGCCACAATGCATCCGGCAAAGGTAATGCGGATGCTACTAGAAAGGCTTCTGCTTATCCTAGACCGTCTGCTTCTCTCCTTCATGTTTGA |
| Protein sequence |
>MsG0780038749.01.T01 MGNANRCPGALVKDMKKLKLLSVILLLMGACWSNKVKSVSPSNTGFTSRSVSRSGHDISSTSRNSSASISVTSRSEGEILQSSNLKSFSYNEVRAATRNFRPDSVLGEGGFGSVFKGWIDEHSHAATKPGMGIIVAVKRLNQDGHQGHREWLAEINYLGQLQHPNLVKLIGYCFEDEHRLLVYEFMPKGSMENHLFRRGSYFQPFSWSLRMKIALGAAKGLAFLHSTEPKVIYRDFKTSNILLDSNYDAKLSDFGLARDGPTGDKSHVSTRVMGTRGYAAPEYLATGINSSGHLTAKSDVYSFGVVLLEIISGRRAIDKNLPSGEHNLVEWAKPYLSNKRRVFRVMDPRLEGQYSHSRAHAAAALASQCLSVEPRIRPNMDEVVKTLEQLQEPKDAQKKGSDHRARNSNLGHNASGKGNADATRKASAYPRPSASLLHV* |
