AlfalfaGEDB | MsG0380015324.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015324.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF07714

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015324.01.T01	XP_013460666.1	98.177	384	7	0	1	384	1	384	0	791

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015324.01.T01	Q06548	67.209	369	115	3	3	371	1	363	0	513

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048205.01	MsG0380015324.01	0.819351	1.215474e-52	9.330034e-50
MsG0080048207.01	MsG0380015324.01	0.810441	1.151157e-50	6.939313e-48
MsG0080048915.01	MsG0380015324.01	0.814687	1.356920e-51	9.166207e-49
MsG0180000267.01	MsG0380015324.01	0.800233	1.586680e-48	7.349576e-46
MsG0180000585.01	MsG0380015324.01	0.808774	2.625199e-50	1.514183e-47
MsG0180000673.01	MsG0380015324.01	0.833007	6.828456e-56	7.763375e-53
MsG0180001663.01	MsG0380015324.01	0.820296	7.393058e-53	5.826745e-50
MsG0180001921.01	MsG0380015324.01	0.831811	1.350928e-55	1.482475e-52
MsG0180002894.01	MsG0380015324.01	0.839465	1.559546e-57	2.155449e-54
MsG0180002912.01	MsG0380015324.01	0.802335	5.891037e-49	2.878238e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015324.01.T01	MTR_3g067795	98.177	384	7	0	1	384	1	384	0.0	791
MsG0380015324.01.T01	MTR_3g067795	98.177	384	7	0	1	384	1	384	0.0	790
MsG0380015324.01.T01	MTR_3g067795	98.355	304	5	0	81	384	1	304	0.0	631
MsG0380015324.01.T01	MTR_3g067795	98.355	304	5	0	81	384	1	304	0.0	630
MsG0380015324.01.T01	MTR_5g079840	72.900	369	99	1	3	370	1	369	0.0	579
MsG0380015324.01.T01	MTR_2g096950	70.556	360	106	0	3	362	1	360	0.0	549
MsG0380015324.01.T01	MTR_7g061660	68.254	378	114	3	3	376	1	376	0.0	526
MsG0380015324.01.T01	MTR_3g067770	72.022	361	93	3	1	361	1	353	0.0	523
MsG0380015324.01.T01	MTR_2g096970	60.278	360	128	3	3	361	1	346	1.53e-160	455
MsG0380015324.01.T01	MTR_5g038870	59.941	337	128	2	45	381	45	374	1.17e-153	439

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015324.01.T01	AT1G07570	67.209	369	115	3	3	371	1	363	0.0	513
MsG0380015324.01.T01	AT1G07570	67.209	369	115	3	3	371	1	363	0.0	513
MsG0380015324.01.T01	AT1G07570	67.209	369	115	3	3	371	1	363	0.0	513
MsG0380015324.01.T01	AT2G28930	67.308	364	117	1	3	366	1	362	2.38e-180	508
MsG0380015324.01.T01	AT2G28930	67.308	364	114	2	3	366	1	359	6.86e-179	504
MsG0380015324.01.T01	AT5G02290	63.788	359	124	2	3	361	1	353	1.14e-177	500
MsG0380015324.01.T01	AT5G02290	63.788	359	124	2	3	361	1	353	1.14e-177	500
MsG0380015324.01.T01	AT1G07570	69.006	342	105	1	30	371	34	374	8.79e-177	499
MsG0380015324.01.T01	AT2G39660	64.641	362	121	2	3	364	1	355	2.11e-176	497
MsG0380015324.01.T01	AT2G28930	65.479	365	112	1	2	366	20	370	3.27e-174	492

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Find 96 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCTCAGATTTCGGGA+TGG	0.696023	3:+68454778	MsG0380015324.01.T01:CDS
AAACCAGTACCTGGTTTAGT+AGG	0.499048	3:-68453958	None:intergenic
AAAGGTTGTCGGGAGAGAAG+AGG	0.643339	3:+68453723	MsG0380015324.01.T01:CDS
AACAGTGGTTCAAAACAGCA+TGG	0.644648	3:+68455667	MsG0380015324.01.T01:CDS
AACGTCGATGAATCTTTAAG+CGG	0.624665	3:+68455693	MsG0380015324.01.T01:exon
AAGAGTGATGTATACAGTTT+TGG	0.244902	3:+68455346	MsG0380015324.01.T01:CDS
AAGAGTGCAATTAGAATAGA+TGG	0.431031	3:-68455859	None:intergenic
AATCAAGAGCTATCTTCATT+CGG	0.480254	3:-68454575	None:intergenic
AATCATGCTCTCCAGACGGT+CGG	0.653401	3:-68455417	None:intergenic
AATCCAATGATAAAACAAAT+GGG	0.528287	3:+68455632	MsG0380015324.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCAAGAGCTATCTTCATT+CGG	-	Chr3:68454578-68454597	None:intergenic	30.0%
AATGCAAAACTCTCAGATTT+CGG	+	Chr3:68454773-68454792	MsG0380015324.01.T01:CDS	30.0%
AATTCTGTGTTCTATGATGA+TGG	+	Chr3:68452782-68452801	MsG0380015324.01.T01:exon	30.0%
ACGTAAGATTTCTCAAGTTA+TGG	+	Chr3:68455474-68455493	MsG0380015324.01.T01:CDS	30.0%
AGTGAAACACTTCAAATAGT+TGG	-	Chr3:68453830-68453849	None:intergenic	30.0%
ATATAGACCAAACATTGAAG+AGG	+	Chr3:68455582-68455601	MsG0380015324.01.T01:CDS	30.0%
ATCATAGAACACAGAATTGT+TGG	-	Chr3:68452780-68452799	None:intergenic	30.0%
ATGATTATCCAAACTTCCTT+TGG	-	Chr3:68454387-68454406	None:intergenic	30.0%
ATTCTGTGTTCTATGATGAT+GGG	+	Chr3:68452783-68452802	MsG0380015324.01.T01:exon	30.0%
ATTTCTGTCTACGAAATACA+AGG	-	Chr3:68454269-68454288	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	68452660	68455896	68452660	ID=MsG0380015324.01;Name=MsG0380015324.01
Chr3	mRNA	68452660	68455896	68452660	ID=MsG0380015324.01.T01;Parent=MsG0380015324.01;Name=MsG0380015324.01.T01;_AED=0.47;_eAED=0.47;_QI=135\|1\|1\|1\|0.4\|0.5\|6\|185\|388
Chr3	exon	68452660	68452852	68452660	ID=MsG0380015324.01.T01:exon:176;Parent=MsG0380015324.01.T01
Chr3	exon	68453713	68454038	68453713	ID=MsG0380015324.01.T01:exon:177;Parent=MsG0380015324.01.T01
Chr3	exon	68454281	68454416	68454281	ID=MsG0380015324.01.T01:exon:178;Parent=MsG0380015324.01.T01
Chr3	exon	68454541	68454683	68454541	ID=MsG0380015324.01.T01:exon:179;Parent=MsG0380015324.01.T01
Chr3	exon	68454767	68454890	68454767	ID=MsG0380015324.01.T01:exon:180;Parent=MsG0380015324.01.T01
Chr3	exon	68455332	68455896	68455332	ID=MsG0380015324.01.T01:exon:181;Parent=MsG0380015324.01.T01
Chr3	five_prime_UTR	68452660	68452794	68452660	ID=MsG0380015324.01.T01:five_prime_utr;Parent=MsG0380015324.01.T01
Chr3	CDS	68452795	68452852	68452795	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	CDS	68453713	68454038	68453713	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	CDS	68454281	68454416	68454281	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	CDS	68454541	68454683	68454541	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	CDS	68454767	68454890	68454767	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	CDS	68455332	68455711	68455332	ID=MsG0380015324.01.T01:cds;Parent=MsG0380015324.01.T01
Chr3	three_prime_UTR	68455712	68455896	68455712	ID=MsG0380015324.01.T01:three_prime_utr;Parent=MsG0380015324.01.T01

Gene Sequence

>MsG0380015324.01.T01

ATGATGATGGGTTGCTGCTTTAGTGCAAAGGTCAAAGCTGAGAGTCCTACACATAATGGTTTGAATTCAAAGGTTGTCGGGAGAGAAGAGGATGTTTCGAGTGGTTTAAACAGAAAGGCCTCTTCTTCATCTGTGCTTCTAAGCTCTAGGACTGAGGGTGAGATATTGCAATCCAACTATTTGAAGTGTTTCACTATCAACGAGGTAAGAGCCGCAACAAGGAACTTTCGTCCAGATAGTATGATTGGCGAAGGTGGATTTGGTTGTGTATTTAAGGGTTGGATCGACGAGCACACACTCGCTCCTACTAAACCAGGTACTGGTTTTGTTATTGCTGTCAAGAGGCTTAACCAAGAAAGCAATCAGGGACATAGTGAATGGTTGACAGAAATCAATTACCTTGGACAGCTGCATCATCCAAATCTAGTAAAACTGATAGGTTACTGCTTAGAGGATGACTACCGGATTTTGGTATATGAATTTGTGACCAAAGGAAGTTTGGATAATCATTTGTTTAGAAGAGCTTCTTACTTTCAGCCACTCTCTTGGAAAATCCGAATGAAGATAGCTCTTGATTCTGCTAAGGGTCTTGCATTTCTTCACAGTGATGAAGTCGAAGTTATATATCGAGACTTCAAAACTTCTAACATCTTGATTGATTCTAATTATAATGCAAAACTCTCAGATTTCGGGATGGCAAAAGATGGACCAGAAGGTGGTAAGAGTCATGTCTCTACAAGAATAATGGGCACCCCTGGCTATGCAGCTCCTGAGTATTTAGCCACAGGTCACCTAACCAAAAAGAGTGATGTATACAGTTTTGGGGTCGTTCTCCTAGAAATTATGTCAGGCAAACGTGTATTGGATAAAAACCGACCGTCTGGAGAGCATGATTTAGTTGCATGGGCCAAGCCATTACTCATGAGCAAACGTAAGATTTCTCAAGTTATGGATGCTCGTATAGAAGGCCAATACTCGTCGCGTGAAGCAATTAAAGTAGCTCATGTTGCAATTCGATGTCTGTCGGCAGAACAAAAATATAGACCAAACATTGAAGAGGTGGTGAGATCGTTGGAACAACTTCAGGAATCCAATGATAAAACAAATGGGCATAGCTCAAGCAACAGTGGTTCAAAACAGCATGGAAAAACGTCGATGAATCTTTAA

Protein sequence

>MsG0380015324.01.T01

MMMGCCFSAKVKAESPTHNGLNSKVVGREEDVSSGLNRKASSSSVLLSSRTEGEILQSNYLKCFTINEVRAATRNFRPDSMIGEGGFGCVFKGWIDEHTLAPTKPGTGFVIAVKRLNQESNQGHSEWLTEINYLGQLHHPNLVKLIGYCLEDDYRILVYEFVTKGSLDNHLFRRASYFQPLSWKIRMKIALDSAKGLAFLHSDEVEVIYRDFKTSNILIDSNYNAKLSDFGMAKDGPEGGKSHVSTRIMGTPGYAAPEYLATGHLTKKSDVYSFGVVLLEIMSGKRVLDKNRPSGEHDLVAWAKPLLMSKRKISQVMDARIEGQYSSREAIKVAHVAIRCLSAEQKYRPNIEEVVRSLEQLQESNDKTNGHSSSNSGSKQHGKTSMNL*