AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038941.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038941.01.T01 MTR_7g065750 83.628 226 9 1 1 198 1 226 1.78e-133 373
MsG0780038941.01.T01 MTR_7g065740 71.239 226 37 1 1 198 1 226 5.37e-110 314
MsG0780038941.01.T01 MTR_7g065720 64.840 219 49 1 1 191 1 219 2.45e-97 282
MsG0780038941.01.T01 MTR_7g065660 62.832 226 55 2 1 198 1 225 3.67e-96 279
MsG0780038941.01.T01 MTR_7g065590 55.357 224 71 2 1 196 1 223 2.19e-82 244
MsG0780038941.01.T01 MTR_7g065640 56.637 226 51 3 1 198 1 207 2.48e-81 241
MsG0780038941.01.T01 MTR_7g065290 58.716 218 63 2 1 191 1 218 9.22e-81 240
MsG0780038941.01.T01 MTR_4g019790 56.637 226 69 3 1 198 1 225 2.34e-80 239
MsG0780038941.01.T01 MTR_7g065600 55.605 223 72 2 1 196 1 223 1.53e-78 234
MsG0780038941.01.T01 MTR_7g065265 57.339 218 66 2 1 191 1 218 1.31e-77 232
MsG0780038941.01.T01 MTR_7g065630 56.444 225 67 3 1 198 19 239 2.86e-75 226
MsG0780038941.01.T01 MTR_7g065270 55.708 219 68 3 1 191 1 218 2.17e-74 224
MsG0780038941.01.T01 MTR_4g019780 54.206 214 71 3 2 188 3 216 7.83e-72 217
MsG0780038941.01.T01 MTR_7g065260 52.444 225 80 3 1 198 8 232 1.02e-70 214
MsG0780038941.01.T01 MTR_7g065230 54.222 225 70 4 1 198 1 219 1.51e-69 211
MsG0780038941.01.T01 MTR_7g065710 53.623 207 65 3 8 187 5 207 2.12e-65 201
MsG0780038941.01.T01 MTR_7g065680 49.778 225 82 4 1 198 1 221 3.90e-60 187
MsG0780038941.01.T01 MTR_7g065700 50.467 214 75 3 1 187 1 210 9.62e-58 181
MsG0780038941.01.T01 MTR_1g090150 39.906 213 100 4 5 189 1 213 9.45e-44 145
MsG0780038941.01.T01 MTR_3g099757 41.818 220 101 5 5 197 3 222 4.65e-43 144
MsG0780038941.01.T01 MTR_8g087410 38.667 225 110 5 1 198 1 224 2.35e-41 139
MsG0780038941.01.T01 MTR_8g087425 38.028 213 105 3 4 189 2 214 1.72e-40 137
MsG0780038941.01.T01 MTR_1g115195 37.089 213 104 2 7 189 3 215 2.10e-40 137
MsG0780038941.01.T01 MTR_6g080440 38.009 221 108 5 7 198 3 223 2.85e-40 137
MsG0780038941.01.T01 MTR_5g040430 37.387 222 104 3 1 188 1 221 1.97e-38 132
MsG0780038941.01.T01 MTR_1g090070 35.747 221 112 4 7 197 9 229 9.19e-38 130
MsG0780038941.01.T01 MTR_5g037380 38.249 217 97 4 1 182 1 215 2.68e-36 127
MsG0780038941.01.T01 MTR_4g059730 35.377 212 108 2 6 188 3 214 4.74e-36 125
MsG0780038941.01.T01 MTR_1g090060 38.942 208 96 6 7 184 3 209 1.02e-35 125
MsG0780038941.01.T01 MTR_1g090090 36.036 222 111 5 7 197 3 224 1.82e-35 124
MsG0780038941.01.T01 MTR_1g090100 36.408 206 102 4 8 184 3 208 1.82e-30 111
MsG0780038941.01.T01 MTR_2g070130 35.407 209 97 7 6 182 3 205 5.80e-30 110
MsG0780038941.01.T01 MTR_2g070150 34.975 203 102 5 6 180 3 203 6.08e-30 109
MsG0780038941.01.T01 MTR_8g061950 32.420 219 113 3 5 188 3 221 1.11e-28 107
MsG0780038941.01.T01 MTR_8g056940 34.703 219 106 6 6 191 3 217 1.05e-27 104
MsG0780038941.01.T01 MTR_2g070120 34.928 209 98 6 6 182 3 205 1.33e-27 104
MsG0780038941.01.T01 MTR_2g070140 33.854 192 97 5 21 184 2 191 2.04e-27 103
MsG0780038941.01.T01 MTR_2g070180 34.171 199 101 5 10 180 7 203 3.52e-27 103
MsG0780038941.01.T01 MTR_3g467420 35.071 211 106 6 10 192 12 219 7.90e-27 102
MsG0780038941.01.T01 MTR_3g467430 35.644 202 100 5 4 177 6 205 8.69e-27 102
MsG0780038941.01.T01 MTR_2g070210 32.692 208 108 6 6 183 3 208 2.59e-26 100
MsG0780038941.01.T01 MTR_4g124130 40.206 194 81 8 5 167 2 191 5.92e-26 99.8
MsG0780038941.01.T01 MTR_2g070200 34.375 192 95 4 6 168 3 192 1.22e-25 99.0
MsG0780038941.01.T01 MTR_2g070060 33.636 220 109 6 7 198 4 214 1.50e-25 99.0
MsG0780038941.01.T01 MTR_5g076900 36.224 196 91 7 15 181 14 204 1.92e-25 98.6
MsG0780038941.01.T01 MTR_2g070070 32.338 201 102 5 7 177 4 200 4.57e-24 95.1
MsG0780038941.01.T01 MTR_0186s0030 57.333 75 32 0 6 80 3 77 7.40e-23 89.7
MsG0780038941.01.T01 MTR_2g070110 33.484 221 107 7 7 198 4 213 5.81e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038941.01.T01 AT3G09270 44.144 222 94 5 4 195 2 223 8.40e-55 174
MsG0780038941.01.T01 AT2G29420 39.726 219 101 3 3 192 5 221 3.34e-50 162
MsG0780038941.01.T01 AT2G29450 41.256 223 102 5 3 196 2 224 3.34e-38 131
MsG0780038941.01.T01 AT2G29490 40.865 208 95 4 1 180 1 208 7.26e-38 130
MsG0780038941.01.T01 AT2G29440 37.838 222 110 5 3 196 2 223 3.79e-37 129
MsG0780038941.01.T01 AT2G29480 38.839 224 109 4 1 196 1 224 4.80e-37 129
MsG0780038941.01.T01 AT2G29460 37.946 224 111 4 1 196 1 224 5.87e-36 125
MsG0780038941.01.T01 AT2G29470 38.756 209 99 5 1 180 1 209 6.38e-34 120
MsG0780038941.01.T01 AT1G59670 36.283 226 109 3 3 193 2 227 5.03e-33 118
MsG0780038941.01.T01 AT1G59700 38.571 210 94 4 3 177 2 211 5.26e-32 115
MsG0780038941.01.T01 AT1G10370 35.266 207 100 4 5 177 3 209 7.03e-32 115
MsG0780038941.01.T01 AT1G27130 32.143 224 117 4 3 191 2 225 8.59e-31 112
MsG0780038941.01.T01 AT1G69920 34.597 211 103 5 2 177 29 239 3.72e-30 111
MsG0780038941.01.T01 AT1G53680 35.514 214 104 5 1 183 1 211 6.77e-30 110
MsG0780038941.01.T01 AT1G69930 32.301 226 120 4 2 194 7 232 7.07e-30 110
MsG0780038941.01.T01 AT1G78380 36.098 205 95 7 7 181 4 202 1.14e-29 109
MsG0780038941.01.T01 AT1G78320 34.286 210 106 5 5 185 2 208 2.60e-29 108
MsG0780038941.01.T01 AT1G78370 32.227 211 112 5 10 192 7 214 2.48e-28 105
MsG0780038941.01.T01 AT1G10360 33.816 207 101 7 6 177 4 209 4.55e-28 105
MsG0780038941.01.T01 AT1G78340 39.752 161 69 3 6 138 3 163 5.31e-28 105
MsG0780038941.01.T01 AT1G27140 32.227 211 107 4 3 177 2 212 6.45e-28 105
MsG0780038941.01.T01 AT5G62480 31.081 222 117 3 10 195 11 232 1.27e-27 104
MsG0780038941.01.T01 AT1G78320 35.870 184 111 5 5 185 2 181 3.57e-27 102
MsG0780038941.01.T01 AT1G74590 32.039 206 108 3 1 174 1 206 2.71e-26 101
MsG0780038941.01.T01 AT1G17180 35.821 201 97 6 6 177 3 200 6.48e-26 99.8
MsG0780038941.01.T01 AT1G17170 34.673 199 101 5 6 177 3 199 2.14e-25 98.6
MsG0780038941.01.T01 AT3G43800 32.547 212 105 8 4 182 2 208 1.02e-23 94.4
MsG0780038941.01.T01 AT1G78360 34.328 201 99 6 7 177 4 201 1.53e-23 93.6
MsG0780038941.01.T01 AT5G62480 31.579 190 118 3 10 188 11 199 3.04e-23 92.8
MsG0780038941.01.T01 AT5G62480 31.053 190 118 4 10 188 11 198 3.89e-21 87.0
MsG0780038941.01.T01 AT1G17190 32.984 191 92 5 10 169 8 193 2.93e-20 85.1

Find 36 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAAGCCCATTTGCTTGC+AGG 0.244780 7:-55577344 MsG0780038941.01.T01:CDS
CCTTTGTTTCAAGATTTAAT+AGG 0.285103 7:-55576703 MsG0780038941.01.T01:CDS
GAGCTGAAGAACAAGTTCTT+TGG 0.292601 7:-55576781 MsG0780038941.01.T01:CDS
ACCGGAACCTTCTTATGAAC+TGG 0.328607 7:+55577231 None:intergenic
GCAATCAATGTCCGCAAATC+AGG 0.336594 7:-55577391 None:intergenic
TGGCTCCATTTGTAAAGCTT+TGG 0.339930 7:+55576652 None:intergenic
AGCTGAAGAACAAGTTCTTT+GGG 0.341432 7:-55576780 MsG0780038941.01.T01:CDS
TTGGATGGTTGAGAAATTCT+TGG 0.345416 7:+55576632 None:intergenic
TATGAACTGGATTGTATTTG+AGG 0.352045 7:+55577244 None:intergenic
CTTTGTTTCAAGATTTAATA+GGG 0.353287 7:-55576702 MsG0780038941.01.T01:CDS
AAGATTTAATAGGGTTGAAA+TGG 0.365583 7:-55576693 MsG0780038941.01.T01:CDS
ATCACAAGGGATTCTGATAT+AGG 0.376717 7:+55577189 None:intergenic
GGCACATTCTCCTTCACAAT+TGG 0.384087 7:+55576613 None:intergenic
TCCAGTTCATAAGAAGGTTC+CGG 0.393726 7:-55577232 MsG0780038941.01.T01:CDS
ATCTGTACCCTGCAAGCAAA+TGG 0.394233 7:+55577336 None:intergenic
TTTCGCCTCTTCCTGATTTG+CGG 0.414011 7:+55577380 None:intergenic
GCATAAACAGCTTTCAATGA+AGG 0.463434 7:+55576874 None:intergenic
GGGAAGCCCATTTGCTTGCA+GGG 0.469066 7:-55577343 MsG0780038941.01.T01:CDS
AGTATCCAAAGCTTTACAAA+TGG 0.475417 7:-55576657 MsG0780038941.01.T01:CDS
CAAATATGTTCAAGAAGACT+TGG 0.481910 7:-55577286 MsG0780038941.01.T01:CDS
CATTCTCCTTCACAATTGGA+TGG 0.491109 7:+55576617 None:intergenic
GGGTACAGATTGCTCTTAAG+TGG 0.499386 7:-55577323 MsG0780038941.01.T01:CDS
CCTATTAAATCTTGAAACAA+AGG 0.519986 7:+55576703 None:intergenic
CTATTAAATCTTGAAACAAA+GGG 0.523551 7:+55576704 None:intergenic
TCTGTACCCTGCAAGCAAAT+GGG 0.529543 7:+55577337 None:intergenic
ATACAATCCAGTTCATAAGA+AGG 0.532878 7:-55577238 MsG0780038941.01.T01:CDS
AATGTCCGCAAATCAGGAAG+AGG 0.575773 7:-55577385 MsG0780038941.01.T01:CDS
TCTCAACCATCCAATTGTGA+AGG 0.577628 7:-55576623 MsG0780038941.01.T01:CDS
CAGATTGCTCTTAAGTGGAA+AGG 0.578095 7:-55577318 MsG0780038941.01.T01:CDS
CAATGTATTCAAGAATCACA+AGG 0.596729 7:+55577175 None:intergenic
GCTGAAGAACAAGTTCTTTG+GGG 0.624895 7:-55576779 MsG0780038941.01.T01:CDS
AAAATATTGAAGAATCAACT+GGG 0.642194 7:-55576825 MsG0780038941.01.T01:CDS
CTGAAGAACAAGTTCTTTGG+GGG 0.643585 7:-55576778 MsG0780038941.01.T01:CDS
GATTCTTGAATACATTGATG+AGG 0.657297 7:-55577169 MsG0780038941.01.T01:intron
AATGTATTCAAGAATCACAA+GGG 0.692745 7:+55577176 None:intergenic
AAATATTGAAGAATCAACTG+GGG 0.718023 7:-55576824 MsG0780038941.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATTGAAGAATCAAC+TGG - Chr7:55577104-55577123 MsG0780038941.01.T01:intron 20.0%
!! AAAATATTGAAGAATCAACT+GGG - Chr7:55577105-55577124 MsG0780038941.01.T01:intron 20.0%
!! CTATTAAATCTTGAAACAAA+GGG + Chr7:55577229-55577248 None:intergenic 20.0%
!!! CTTTGTTTCAAGATTTAATA+GGG - Chr7:55577228-55577247 MsG0780038941.01.T01:CDS 20.0%
! AAATATTGAAGAATCAACTG+GGG - Chr7:55577106-55577125 MsG0780038941.01.T01:intron 25.0%
! AAGATTTAATAGGGTTGAAA+TGG - Chr7:55577237-55577256 MsG0780038941.01.T01:CDS 25.0%
! AATGTATTCAAGAATCACAA+GGG + Chr7:55576757-55576776 None:intergenic 25.0%
! CCTATTAAATCTTGAAACAA+AGG + Chr7:55577230-55577249 None:intergenic 25.0%
!! CCTTTGTTTCAAGATTTAAT+AGG - Chr7:55577227-55577246 MsG0780038941.01.T01:CDS 25.0%
AGTATCCAAAGCTTTACAAA+TGG - Chr7:55577273-55577292 MsG0780038941.01.T01:CDS 30.0%
ATACAATCCAGTTCATAAGA+AGG - Chr7:55576692-55576711 MsG0780038941.01.T01:CDS 30.0%
CAAATATGTTCAAGAAGACT+TGG - Chr7:55576644-55576663 MsG0780038941.01.T01:CDS 30.0%
CAATGTATTCAAGAATCACA+AGG + Chr7:55576758-55576777 None:intergenic 30.0%
CCTTGAAATTTGCAAAAAGA+GGG + Chr7:55577352-55577371 None:intergenic 30.0%
GATTCTTGAATACATTGATG+AGG - Chr7:55576761-55576780 MsG0780038941.01.T01:CDS 30.0%
TATGAACTGGATTGTATTTG+AGG + Chr7:55576689-55576708 None:intergenic 30.0%
TTCTCATTGTGAACAAAAAC+CGG + Chr7:55576720-55576739 None:intergenic 30.0%
TTGAATACATTGATGAGGTT+TGG - Chr7:55576766-55576785 MsG0780038941.01.T01:CDS 30.0%
! AAAATCGAGCCAATGATTTT+TGG + Chr7:55576824-55576843 None:intergenic 30.0%
!!! AAAAATCATTGGCTCGATTT+TGG - Chr7:55576823-55576842 MsG0780038941.01.T01:CDS 30.0%
!!! CAATGATTTTTGGTATGGAT+CGG + Chr7:55576814-55576833 None:intergenic 30.0%
AAAATGTAGCGATTGCGATT+TGG + Chr7:55576923-55576942 None:intergenic 35.0%
AAATGTAGCGATTGCGATTT+GGG + Chr7:55576922-55576941 None:intergenic 35.0%
AGCTGAAGAACAAGTTCTTT+GGG - Chr7:55577150-55577169 MsG0780038941.01.T01:intron 35.0%
ATCACAAGGGATTCTGATAT+AGG + Chr7:55576744-55576763 None:intergenic 35.0%
CGATCCATACCAAAAATCAT+TGG - Chr7:55576812-55576831 MsG0780038941.01.T01:CDS 35.0%
CGTGCATAGAAATTGTAATG+CGG + Chr7:55576869-55576888 None:intergenic 35.0%
GCATAAACAGCTTTCAATGA+AGG + Chr7:55577059-55577078 None:intergenic 35.0%
GCCTTGAAATTTGCAAAAAG+AGG + Chr7:55577353-55577372 None:intergenic 35.0%
TTGGATGGTTGAGAAATTCT+TGG + Chr7:55577301-55577320 None:intergenic 35.0%
! CCCTCTTTTTGCAAATTTCA+AGG - Chr7:55577349-55577368 MsG0780038941.01.T01:CDS 35.0%
CAGATTGCTCTTAAGTGGAA+AGG - Chr7:55576612-55576631 MsG0780038941.01.T01:CDS 40.0%
CATTCTCCTTCACAATTGGA+TGG + Chr7:55577316-55577335 None:intergenic 40.0%
CTGAAGAACAAGTTCTTTGG+GGG - Chr7:55577152-55577171 MsG0780038941.01.T01:intron 40.0%
GAGCTGAAGAACAAGTTCTT+TGG - Chr7:55577149-55577168 MsG0780038941.01.T01:intron 40.0%
GCTGAAGAACAAGTTCTTTG+GGG - Chr7:55577151-55577170 MsG0780038941.01.T01:intron 40.0%
TCCAGTTCATAAGAAGGTTC+CGG - Chr7:55576698-55576717 MsG0780038941.01.T01:CDS 40.0%
TCTCAACCATCCAATTGTGA+AGG - Chr7:55577307-55577326 MsG0780038941.01.T01:CDS 40.0%
TGGCTCCATTTGTAAAGCTT+TGG + Chr7:55577281-55577300 None:intergenic 40.0%
! CTGCTGTGTTCATAGCTTTT+TGG - Chr7:55577201-55577220 MsG0780038941.01.T01:CDS 40.0%
! TGGTCGAAATTCATCGATTG+TGG - Chr7:55576843-55576862 MsG0780038941.01.T01:CDS 40.0%
!! GAGGAGAAGATCACTTTTGT+TGG + Chr7:55576670-55576689 None:intergenic 40.0%
!!! CGAGCCAATGATTTTTGGTA+TGG + Chr7:55576819-55576838 None:intergenic 40.0%
!!! GATTTTTGGTATGGATCGGA+CGG + Chr7:55576810-55576829 None:intergenic 40.0%
ACCGGAACCTTCTTATGAAC+TGG + Chr7:55576702-55576721 None:intergenic 45.0%
ATCTGTACCCTGCAAGCAAA+TGG + Chr7:55576597-55576616 None:intergenic 45.0%
GCAAAAAGAGGGTCTCTAAG+AGG + Chr7:55577341-55577360 None:intergenic 45.0%
GGCACATTCTCCTTCACAAT+TGG + Chr7:55577320-55577339 None:intergenic 45.0%
GGGTACAGATTGCTCTTAAG+TGG - Chr7:55576607-55576626 MsG0780038941.01.T01:CDS 45.0%
TATGGATCGGACGGCAAAAT+GGG + Chr7:55576801-55576820 None:intergenic 45.0%
TCTGTACCCTGCAAGCAAAT+GGG + Chr7:55576596-55576615 None:intergenic 45.0%
! CAGGAAGAGGCGAAACTTTT+AGG - Chr7:55576558-55576577 MsG0780038941.01.T01:CDS 45.0%
! TTCTTTGGGGGAGAAGAGAT+TGG - Chr7:55577164-55577183 MsG0780038941.01.T01:intron 45.0%
! TTTCGCCTCTTCCTGATTTG+CGG + Chr7:55576553-55576572 None:intergenic 45.0%
!! GCGAAACTTTTAGGAGCTCT+GGG - Chr7:55576567-55576586 MsG0780038941.01.T01:CDS 45.0%
!!! GGGAGAAGAGATTGGTTTTG+TGG - Chr7:55577172-55577191 MsG0780038941.01.T01:CDS 45.0%
GTATGGATCGGACGGCAAAA+TGG + Chr7:55576802-55576821 None:intergenic 50.0%
!! GGCGAAACTTTTAGGAGCTC+TGG - Chr7:55576566-55576585 MsG0780038941.01.T01:CDS 50.0%
GGGAAGCCCATTTGCTTGCA+GGG - Chr7:55576587-55576606 MsG0780038941.01.T01:CDS 55.0%
TGGGAAGCCCATTTGCTTGC+AGG - Chr7:55576586-55576605 MsG0780038941.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 55576546 55577406 55576546 ID=MsG0780038941.01;Name=MsG0780038941.01
Chr7 mRNA 55576546 55577406 55576546 ID=MsG0780038941.01.T01;Parent=MsG0780038941.01;Name=MsG0780038941.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|198
Chr7 exon 55577170 55577406 55577170 ID=MsG0780038941.01.T01:exon:7795;Parent=MsG0780038941.01.T01
Chr7 exon 55576546 55576905 55576546 ID=MsG0780038941.01.T01:exon:7794;Parent=MsG0780038941.01.T01
Chr7 CDS 55577170 55577406 55577170 ID=MsG0780038941.01.T01:cds;Parent=MsG0780038941.01.T01
Chr7 CDS 55576546 55576905 55576546 ID=MsG0780038941.01.T01:cds;Parent=MsG0780038941.01.T01
Gene Sequence

>MsG0780038941.01.T01

ATGTCCGCAAATCAGGAAGAGGCGAAACTTTTAGGAGCTCTGGGAAGCCCATTTGCTTGCAGGGTACAGATTGCTCTTAAGTGGAAAGGAGTTGAATACAAATATGTTCAAGAAGACTTGGCCAACAAAAGTGATCTTCTCCTCAAATACAATCCAGTTCATAAGAAGGTTCCGGTTTTTGTTCACAATGAGAAACCTATATCAGAATCCCTTGTGATTCTTGAATACATTGATGAGATTGCAACTCCTTCATTGAAAGCTGTTTATGCTTTTAACGATGAGAAAGAGCGCGAGAAAAATATTGAAGAATCAACTGGGGCTCTGCAGATTCTTGAGAATGAGCTGAAGAACAAGTTCTTTGGGGGAGAAGAGATTGGTTTTGTGGATATTGCTGCTGTGTTCATAGCTTTTTGGATCCCTTTGTTTCAAGATTTAATAGGGTTGAAATGGTTCACTGCTGAAAAGTATCCAAAGCTTTACAAATGGAGCCAAGAATTTCTCAACCATCCAATTGTGAAGGAGAATGTGCCTCTTAGAGACCCTCTTTTTGCAAATTTCAAGGCTAGATATGATAGAGCTGTTTCTGCTTCAAAATAG

Protein sequence

>MsG0780038941.01.T01

MSANQEEAKLLGALGSPFACRVQIALKWKGVEYKYVQEDLANKSDLLLKYNPVHKKVPVFVHNEKPISESLVILEYIDEIATPSLKAVYAFNDEKEREKNIEESTGALQILENELKNKFFGGEEIGFVDIAAVFIAFWIPLFQDLIGLKWFTAEKYPKLYKWSQEFLNHPIVKENVPLRDPLFANFKARYDRAVSASK*