Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012702.01.T01 | RHN73858.1 | 63.514 | 222 | 68 | 4 | 1 | 218 | 157 | 369 | 1.10E-85 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012702.01.T01 | Q6DR20 | 34.934 | 229 | 129 | 7 | 5 | 217 | 161 | 385 | 3.95E-34 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012702.01.T01 | A0A396JBS2 | 63.514 | 222 | 68 | 4 | 1 | 218 | 157 | 369 | 5.26e-86 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0380012702.01 | 0.800357 | 1.496996e-48 | 6.956258e-46 |
| MsG0080048874.01 | MsG0380012702.01 | 0.819570 | 1.083872e-52 | 8.371614e-50 |
| MsG0180000470.01 | MsG0380012702.01 | 0.829577 | 4.761643e-55 | 4.893088e-52 |
| MsG0180001063.01 | MsG0380012702.01 | 0.803478 | 3.420136e-49 | 1.720472e-46 |
| MsG0180001410.01 | MsG0380012702.01 | 0.827542 | 1.476714e-54 | 1.429912e-51 |
| MsG0180001971.01 | MsG0380012702.01 | 0.800271 | 1.558368e-48 | 7.225573e-46 |
| MsG0180002089.01 | MsG0380012702.01 | 0.808607 | 2.849498e-50 | 1.636415e-47 |
| MsG0180003669.01 | MsG0380012702.01 | 0.833639 | 4.751857e-56 | 5.505045e-53 |
| MsG0180003686.01 | MsG0380012702.01 | 0.803094 | 4.106326e-49 | 2.045286e-46 |
| MsG0180004264.01 | MsG0380012702.01 | 0.801871 | 7.338987e-49 | 3.543596e-46 |
| MsG0280006324.01 | MsG0380012702.01 | 0.803445 | 3.475108e-49 | 1.746620e-46 |
| MsG0280010796.01 | MsG0380012702.01 | 0.805006 | 1.643939e-49 | 8.601291e-47 |
| MsG0280011032.01 | MsG0380012702.01 | 0.816939 | 4.269067e-52 | 3.067007e-49 |
| MsG0380011759.01 | MsG0380012702.01 | 0.802032 | 6.798727e-49 | 3.296095e-46 |
| MsG0380012702.01 | MsG0380014573.01 | 0.813502 | 2.478464e-51 | 1.621403e-48 |
| MsG0380012702.01 | MsG0380015720.01 | 0.802345 | 5.862978e-49 | 2.865285e-46 |
| MsG0380012702.01 | MsG0380016004.01 | 0.805520 | 1.283155e-49 | 6.802635e-47 |
| MsG0380012702.01 | MsG0380016064.01 | 0.815265 | 1.009863e-51 | 6.929548e-49 |
| MsG0380012702.01 | MsG0380016475.01 | 0.842433 | 2.594054e-58 | 3.933051e-55 |
| MsG0380012702.01 | MsG0380016910.01 | 0.820598 | 6.304239e-53 | 5.009854e-50 |
| MsG0380012702.01 | MsG0380017233.01 | 0.812455 | 4.203981e-51 | 2.674335e-48 |
| MsG0380012702.01 | MsG0380017326.01 | 0.805079 | 1.587368e-49 | 8.320916e-47 |
| MsG0380012702.01 | MsG0480018236.01 | 0.820407 | 6.971740e-53 | 5.511935e-50 |
| MsG0380012702.01 | MsG0480018318.01 | 0.810130 | 1.343212e-50 | 8.031433e-48 |
| MsG0380012702.01 | MsG0480018964.01 | 0.817215 | 3.701271e-52 | 2.679062e-49 |
| MsG0380012702.01 | MsG0480020634.01 | 0.807763 | 4.312182e-50 | 2.422178e-47 |
| MsG0380012702.01 | MsG0480021558.01 | 0.802225 | 6.207719e-49 | 3.024278e-46 |
| MsG0380012702.01 | MsG0480021853.01 | 0.823322 | 1.473927e-53 | 1.264913e-50 |
| MsG0380012702.01 | MsG0480022068.01 | 0.805691 | 1.181151e-49 | 6.290079e-47 |
| MsG0380012702.01 | MsG0480022361.01 | 0.801033 | 1.089889e-48 | 5.152331e-46 |
| MsG0380012702.01 | MsG0480022533.01 | 0.801491 | 8.783477e-49 | 4.200275e-46 |
| MsG0380012702.01 | MsG0480022615.01 | -0.809580 | 1.763720e-50 | 1.039511e-47 |
| MsG0380012702.01 | MsG0480022827.01 | 0.801516 | 8.677614e-49 | 4.152359e-46 |
| MsG0380012702.01 | MsG0480023248.01 | 0.823324 | 1.472430e-53 | 1.263678e-50 |
| MsG0380012702.01 | MsG0480023654.01 | 0.815759 | 7.843067e-52 | 5.455461e-49 |
| MsG0380012702.01 | MsG0580024363.01 | -0.813660 | 2.287750e-51 | 1.503023e-48 |
| MsG0380012702.01 | MsG0580027688.01 | 0.805027 | 1.627068e-49 | 8.517582e-47 |
| MsG0380012702.01 | MsG0580027922.01 | 0.806401 | 8.376549e-50 | 4.542140e-47 |
| MsG0380012702.01 | MsG0580028527.01 | 0.814808 | 1.275867e-51 | 8.646931e-49 |
| MsG0380012702.01 | MsG0580029554.01 | 0.837394 | 5.335132e-57 | 6.920218e-54 |
| MsG0380012702.01 | MsG0680031379.01 | 0.819831 | 9.444898e-53 | 7.348410e-50 |
| MsG0380012702.01 | MsG0680032801.01 | 0.815655 | 8.273324e-52 | 5.738263e-49 |
| MsG0380012702.01 | MsG0680033228.01 | 0.804500 | 2.096660e-49 | 1.082723e-46 |
| MsG0380012702.01 | MsG0680033379.01 | 0.826489 | 2.637599e-54 | 2.477071e-51 |
| MsG0380012702.01 | MsG0780035948.01 | 0.802153 | 6.420286e-49 | 3.122263e-46 |
| MsG0380012702.01 | MsG0780036498.01 | 0.812069 | 5.104140e-51 | 3.212980e-48 |
| MsG0380012702.01 | MsG0780037457.01 | 0.822101 | 2.836300e-53 | 2.351145e-50 |
| MsG0380012702.01 | MsG0780038386.01 | 0.819848 | 9.361305e-53 | 7.286783e-50 |
| MsG0380012702.01 | MsG0780039829.01 | 0.805621 | 1.221963e-49 | 6.495280e-47 |
| MsG0380012702.01 | MsG0780039867.01 | -0.805763 | 1.141064e-49 | 6.087734e-47 |
| MsG0380012702.01 | MsG0780040576.01 | -0.832437 | 9.459446e-56 | 1.057265e-52 |
| MsG0380012702.01 | MsG0780040596.01 | 0.825782 | 3.885194e-54 | 3.576397e-51 |
| MsG0380012702.01 | MsG0880042800.01 | 0.835101 | 2.041345e-56 | 2.470867e-53 |
| MsG0380012702.01 | MsG0880042966.01 | 0.806072 | 9.822164e-50 | 5.281707e-47 |
| MsG0380012702.01 | MsG0880043941.01 | 0.803193 | 3.917645e-49 | 1.956402e-46 |
| MsG0380012702.01 | MsG0880044542.01 | 0.805670 | 1.193446e-49 | 6.351811e-47 |
| MsG0380012702.01 | MsG0880044554.01 | 0.813445 | 2.550595e-51 | 1.666048e-48 |
| MsG0380012702.01 | MsG0880044572.01 | 0.817377 | 3.404200e-52 | 2.475061e-49 |
| MsG0380012702.01 | MsG0880044573.01 | 0.815455 | 9.166301e-52 | 6.322882e-49 |
| MsG0380012702.01 | MsG0880044574.01 | 0.809638 | 1.713939e-50 | 1.011739e-47 |
| MsG0380012702.01 | MsG0880046531.01 | 0.804669 | 1.932920e-49 | 1.002584e-46 |
| MsG0380012702.01 | MsG0880047191.01 | 0.805029 | 1.625892e-49 | 8.511664e-47 |
| MsG0280006456.01 | MsG0380012702.01 | 0.810869 | 9.301120e-51 | 5.671741e-48 |
| MsG0280007072.01 | MsG0380012702.01 | 0.800041 | 1.735481e-48 | 7.999823e-46 |
| MsG0280008182.01 | MsG0380012702.01 | 0.809366 | 1.960730e-50 | 1.149029e-47 |
| MsG0280008615.01 | MsG0380012702.01 | 0.809028 | 2.317094e-50 | 1.345582e-47 |
| MsG0280008894.01 | MsG0380012702.01 | 0.813320 | 2.716612e-51 | 1.768411e-48 |
| MsG0280010443.01 | MsG0380012702.01 | 0.812725 | 3.669663e-51 | 2.350976e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012702.01.T01 | MTR_2g047180 | 63.514 | 222 | 68 | 4 | 1 | 218 | 155 | 367 | 1.40e-89 | 268 |
| MsG0380012702.01.T01 | MTR_1g007260 | 65.728 | 213 | 62 | 6 | 3 | 212 | 179 | 383 | 4.93e-84 | 254 |
| MsG0380012702.01.T01 | MTR_6g018360 | 62.281 | 228 | 72 | 6 | 1 | 219 | 161 | 383 | 3.75e-81 | 248 |
| MsG0380012702.01.T01 | MTR_6g018315 | 62.281 | 228 | 72 | 6 | 1 | 219 | 146 | 368 | 4.39e-80 | 245 |
| MsG0380012702.01.T01 | MTR_1g026850 | 63.134 | 217 | 75 | 3 | 3 | 217 | 151 | 364 | 1.20e-79 | 243 |
| MsG0380012702.01.T01 | MTR_3g030080 | 55.947 | 227 | 81 | 3 | 1 | 220 | 161 | 375 | 3.17e-79 | 242 |
| MsG0380012702.01.T01 | MTR_6g027100 | 60.633 | 221 | 74 | 6 | 1 | 219 | 169 | 378 | 6.35e-78 | 240 |
| MsG0380012702.01.T01 | MTR_6g018390 | 61.290 | 217 | 69 | 5 | 2 | 216 | 162 | 365 | 9.68e-78 | 238 |
| MsG0380012702.01.T01 | MTR_6g027040 | 59.729 | 221 | 76 | 6 | 1 | 219 | 169 | 378 | 3.26e-77 | 237 |
| MsG0380012702.01.T01 | MTR_3g029650 | 57.399 | 223 | 78 | 5 | 6 | 220 | 151 | 364 | 2.30e-72 | 234 |
| MsG0380012702.01.T01 | MTR_3g029650 | 47.345 | 226 | 105 | 6 | 1 | 218 | 532 | 751 | 3.10e-53 | 182 |
| MsG0380012702.01.T01 | MTR_6g018400 | 55.405 | 222 | 77 | 5 | 1 | 216 | 163 | 368 | 2.33e-68 | 214 |
| MsG0380012702.01.T01 | MTR_3g091990 | 53.604 | 222 | 86 | 6 | 4 | 218 | 176 | 387 | 2.02e-65 | 208 |
| MsG0380012702.01.T01 | MTR_3g030360 | 53.670 | 218 | 69 | 6 | 1 | 218 | 167 | 352 | 1.92e-64 | 203 |
| MsG0380012702.01.T01 | MTR_3g030380 | 51.802 | 222 | 85 | 6 | 1 | 218 | 155 | 358 | 1.40e-63 | 201 |
| MsG0380012702.01.T01 | MTR_3g029750 | 50.661 | 227 | 88 | 7 | 1 | 218 | 176 | 387 | 4.82e-63 | 201 |
| MsG0380012702.01.T01 | MTR_3g029670 | 50.000 | 226 | 91 | 6 | 1 | 218 | 176 | 387 | 5.54e-61 | 196 |
| MsG0380012702.01.T01 | MTR_3g030440 | 50.909 | 220 | 85 | 7 | 1 | 216 | 154 | 354 | 7.54e-61 | 194 |
| MsG0380012702.01.T01 | MTR_3g030480 | 50.909 | 220 | 85 | 7 | 1 | 216 | 153 | 353 | 9.82e-61 | 194 |
| MsG0380012702.01.T01 | MTR_1g007220 | 56.500 | 200 | 50 | 5 | 13 | 209 | 2 | 167 | 2.19e-60 | 187 |
| MsG0380012702.01.T01 | MTR_3g092000 | 54.054 | 222 | 83 | 8 | 5 | 218 | 190 | 400 | 4.00e-58 | 189 |
| MsG0380012702.01.T01 | MTR_1g007250 | 65.868 | 167 | 47 | 5 | 46 | 209 | 1 | 160 | 1.49e-57 | 179 |
| MsG0380012702.01.T01 | MTR_1g016570 | 49.123 | 228 | 80 | 7 | 1 | 218 | 160 | 361 | 4.45e-57 | 185 |
| MsG0380012702.01.T01 | MTR_3g030355 | 48.945 | 237 | 85 | 8 | 3 | 218 | 174 | 395 | 4.69e-57 | 186 |
| MsG0380012702.01.T01 | MTR_5g042230 | 58.602 | 186 | 59 | 6 | 32 | 207 | 13 | 190 | 1.74e-56 | 178 |
| MsG0380012702.01.T01 | MTR_3g029620 | 48.661 | 224 | 91 | 7 | 6 | 218 | 164 | 374 | 6.35e-56 | 182 |
| MsG0380012702.01.T01 | MTR_3g029640 | 48.402 | 219 | 92 | 6 | 7 | 217 | 159 | 364 | 2.54e-55 | 180 |
| MsG0380012702.01.T01 | MTR_3g030050 | 43.023 | 258 | 101 | 7 | 1 | 217 | 169 | 421 | 6.68e-55 | 181 |
| MsG0380012702.01.T01 | MTR_3g037710 | 45.064 | 233 | 106 | 7 | 1 | 219 | 41 | 265 | 9.20e-54 | 174 |
| MsG0380012702.01.T01 | MTR_1g049170 | 48.198 | 222 | 94 | 8 | 4 | 216 | 174 | 383 | 1.09e-53 | 177 |
| MsG0380012702.01.T01 | MTR_3g029920 | 47.368 | 228 | 93 | 8 | 3 | 218 | 168 | 380 | 1.55e-53 | 176 |
| MsG0380012702.01.T01 | MTR_6g018370 | 65.772 | 149 | 46 | 4 | 6 | 154 | 108 | 251 | 2.51e-53 | 172 |
| MsG0380012702.01.T01 | MTR_3g030560 | 47.511 | 221 | 98 | 7 | 5 | 218 | 165 | 374 | 2.44e-51 | 171 |
| MsG0380012702.01.T01 | MTR_1g016540 | 50.811 | 185 | 74 | 3 | 46 | 218 | 1 | 180 | 2.97e-50 | 162 |
| MsG0380012702.01.T01 | MTR_3g029800 | 45.000 | 220 | 101 | 7 | 5 | 218 | 152 | 357 | 7.49e-50 | 166 |
| MsG0380012702.01.T01 | MTR_3g029900 | 46.977 | 215 | 94 | 6 | 4 | 217 | 263 | 458 | 1.67e-48 | 166 |
| MsG0380012702.01.T01 | MTR_3g029840 | 48.372 | 215 | 90 | 8 | 4 | 218 | 163 | 356 | 1.73e-48 | 162 |
| MsG0380012702.01.T01 | MTR_3g029950 | 44.889 | 225 | 95 | 6 | 4 | 218 | 163 | 368 | 5.07e-48 | 161 |
| MsG0380012702.01.T01 | MTR_3g029820 | 52.601 | 173 | 65 | 6 | 52 | 218 | 167 | 328 | 1.25e-45 | 154 |
| MsG0380012702.01.T01 | MTR_3g047980 | 44.796 | 221 | 90 | 9 | 1 | 217 | 168 | 360 | 1.74e-45 | 155 |
| MsG0380012702.01.T01 | MTR_3g058460 | 44.660 | 206 | 102 | 5 | 22 | 217 | 173 | 376 | 2.97e-41 | 144 |
| MsG0380012702.01.T01 | MTR_5g078450 | 56.738 | 141 | 37 | 4 | 80 | 218 | 1 | 119 | 6.79e-37 | 125 |
| MsG0380012702.01.T01 | MTR_7g005990 | 40.625 | 224 | 93 | 7 | 5 | 218 | 165 | 358 | 5.29e-36 | 130 |
| MsG0380012702.01.T01 | MTR_3g029870 | 41.850 | 227 | 74 | 9 | 4 | 218 | 155 | 335 | 2.45e-35 | 127 |
| MsG0380012702.01.T01 | MTR_4g022880 | 42.353 | 170 | 77 | 4 | 57 | 217 | 15 | 172 | 6.85e-32 | 115 |
| MsG0380012702.01.T01 | MTR_1g007200 | 75.949 | 79 | 17 | 1 | 126 | 202 | 16 | 94 | 1.86e-29 | 107 |
| MsG0380012702.01.T01 | MTR_8g074610 | 32.653 | 196 | 111 | 6 | 32 | 216 | 210 | 395 | 4.04e-22 | 93.6 |
| MsG0380012702.01.T01 | MTR_8g032080 | 66.154 | 65 | 20 | 1 | 1 | 65 | 37 | 99 | 1.28e-20 | 88.6 |
| MsG0380012702.01.T01 | MTR_7g012990 | 58.228 | 79 | 32 | 1 | 34 | 112 | 76 | 153 | 4.20e-19 | 81.3 |
| MsG0380012702.01.T01 | MTR_2g041510 | 31.915 | 188 | 113 | 5 | 32 | 210 | 183 | 364 | 4.42e-19 | 84.7 |
| MsG0380012702.01.T01 | MTR_2g041510 | 31.915 | 188 | 113 | 5 | 32 | 210 | 203 | 384 | 5.74e-19 | 84.7 |
| MsG0380012702.01.T01 | MTR_8g035995 | 53.763 | 93 | 32 | 5 | 63 | 153 | 9 | 92 | 2.49e-18 | 79.0 |
| MsG0380012702.01.T01 | MTR_8g074640 | 30.964 | 197 | 112 | 7 | 32 | 216 | 191 | 375 | 3.13e-16 | 76.6 |
| MsG0380012702.01.T01 | MTR_5g079830 | 30.189 | 159 | 100 | 5 | 20 | 174 | 166 | 317 | 4.74e-13 | 67.8 |
| MsG0380012702.01.T01 | MTR_3g029850 | 39.326 | 89 | 48 | 2 | 133 | 217 | 31 | 117 | 5.15e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012702.01.T01 | AT2G17036 | 34.934 | 229 | 129 | 7 | 5 | 217 | 161 | 385 | 4.03e-35 | 129 |
| MsG0380012702.01.T01 | AT2G17036 | 34.934 | 229 | 129 | 7 | 5 | 217 | 160 | 384 | 4.40e-35 | 128 |
| MsG0380012702.01.T01 | AT2G17030 | 34.979 | 243 | 131 | 7 | 1 | 220 | 156 | 394 | 4.54e-33 | 123 |
| MsG0380012702.01.T01 | AT5G60060 | 33.714 | 175 | 99 | 7 | 1 | 167 | 164 | 329 | 6.41e-14 | 70.1 |
Find 50 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGAATATTCAGGATATT+TGG | 0.162233 | 3:-22971158 | None:intergenic |
| TCTTGCAAGTATTAATTTCT+TGG | 0.210109 | 3:+22970913 | MsG0380012702.01.T01:CDS |
| CATAACAATTTAGAATATTC+AGG | 0.267633 | 3:-22971166 | None:intergenic |
| GGGTGAAGTTGGCGAATTTA+GGG | 0.273559 | 3:+22970978 | MsG0380012702.01.T01:CDS |
| TTTCAATGGGTCGCCTTATC+TGG | 0.284858 | 3:+22970724 | MsG0380012702.01.T01:CDS |
| GTGGCTGAACCTTTGGTTGA+TGG | 0.316763 | 3:+22970803 | MsG0380012702.01.T01:CDS |
| TGGGTGAAGTTGGCGAATTT+AGG | 0.324049 | 3:+22970977 | MsG0380012702.01.T01:CDS |
| AGGGAATTGTGTGATCATTA+TGG | 0.329701 | 3:+22971069 | MsG0380012702.01.T01:CDS |
| CTGAATATTCTAAATTGTTA+TGG | 0.344183 | 3:+22971167 | MsG0380012702.01.T01:CDS |
| CCAGTTAGTGGCTGAACCTT+TGG | 0.355761 | 3:+22970796 | MsG0380012702.01.T01:CDS |
| TCCTGACATGTCGCCTTGCT+CGG | 0.359645 | 3:+22970688 | MsG0380012702.01.T01:CDS |
| CCAAAGGTTCAGCCACTAAC+TGG | 0.369613 | 3:-22970796 | None:intergenic |
| CAGATTTGTGTGTTGCTAAA+GGG | 0.387719 | 3:+22971050 | MsG0380012702.01.T01:CDS |
| GGAGAAGAAGTGGGTGAAGT+TGG | 0.400739 | 3:+22970967 | MsG0380012702.01.T01:CDS |
| TGTGTTTAAGCTTAATGAAA+AGG | 0.411651 | 3:+22970946 | MsG0380012702.01.T01:CDS |
| CCTTATCTGGTAGACAAAAC+TGG | 0.412169 | 3:+22970737 | MsG0380012702.01.T01:CDS |
| TGAAATGCGGCGATGAGAAT+TGG | 0.427942 | 3:+22970657 | MsG0380012702.01.T01:CDS |
| TGGCGGGAAAAGAAAATTCT+TGG | 0.433576 | 3:+22970826 | MsG0380012702.01.T01:CDS |
| CTAAGCGTGCCGAGTATGAT+AGG | 0.435525 | 3:-22970605 | None:intergenic |
| CCCGAGCAAGGCGACATGTC+AGG | 0.457783 | 3:-22970689 | None:intergenic |
| GAACCTTTGGTTGATGGTGG+CGG | 0.460372 | 3:+22970809 | MsG0380012702.01.T01:CDS |
| TCGCCGCATTTCAACAGTAC+TGG | 0.461047 | 3:-22970647 | None:intergenic |
| AAAACAGCTTCCACATAAAG+TGG | 0.472168 | 3:-22971101 | None:intergenic |
| CCTGACATGTCGCCTTGCTC+GGG | 0.472811 | 3:+22970689 | MsG0380012702.01.T01:CDS |
| CAAGTATTAATTTCTTGGTA+CGG | 0.473932 | 3:+22970918 | MsG0380012702.01.T01:CDS |
| TTGGCGATCCACTCTGGAGG+TGG | 0.481012 | 3:-22971190 | None:intergenic |
| GATAATATATCCACTTTATG+TGG | 0.485429 | 3:+22971091 | MsG0380012702.01.T01:CDS |
| AACCTTTGGTTGATGGTGGC+GGG | 0.492093 | 3:+22970810 | MsG0380012702.01.T01:CDS |
| CGACTCCACTGTCCAGTTAG+TGG | 0.503077 | 3:+22970784 | MsG0380012702.01.T01:CDS |
| TCAGATTTGTGTGTTGCTAA+AGG | 0.511112 | 3:+22971049 | MsG0380012702.01.T01:CDS |
| TATGAGTCTTTAGAGTTCGA+TGG | 0.511537 | 3:+22970884 | MsG0380012702.01.T01:CDS |
| AAAATTCTTGGTGGAGAGTG+AGG | 0.513659 | 3:+22970838 | MsG0380012702.01.T01:CDS |
| AACTGGACAGTGGAGTCGTC+CGG | 0.520658 | 3:-22970779 | None:intergenic |
| GATAAGGCGACCCATTGAAA+AGG | 0.555789 | 3:-22970721 | None:intergenic |
| TTAATGAAAAGGAGAAGAAG+TGG | 0.562031 | 3:+22970957 | MsG0380012702.01.T01:CDS |
| CGGGAAAAGAAAATTCTTGG+TGG | 0.567651 | 3:+22970829 | MsG0380012702.01.T01:CDS |
| TTCCCGCCACCATCAACCAA+AGG | 0.571866 | 3:-22970812 | None:intergenic |
| AGGCAGATGTCTCCCGAGCA+AGG | 0.579393 | 3:-22970701 | None:intergenic |
| AAATTCTTGGTGGAGAGTGA+GGG | 0.579999 | 3:+22970839 | MsG0380012702.01.T01:CDS |
| TAATGAAAAGGAGAAGAAGT+GGG | 0.584827 | 3:+22970958 | MsG0380012702.01.T01:CDS |
| GGTGAAGTTGGCGAATTTAG+GGG | 0.608400 | 3:+22970979 | MsG0380012702.01.T01:CDS |
| TGAGGGTGAGTTATTGCTAA+CGG | 0.631275 | 3:+22970856 | MsG0380012702.01.T01:CDS |
| GCTGAACCTTTGGTTGATGG+TGG | 0.633853 | 3:+22970806 | MsG0380012702.01.T01:CDS |
| ACGGACGGTTACTGTTAGAC+CGG | 0.638236 | 3:+22970760 | MsG0380012702.01.T01:CDS |
| GGTAGACAAAACTGGACGGA+CGG | 0.641749 | 3:+22970745 | MsG0380012702.01.T01:CDS |
| ATGCGGCGATGAGAATTGGA+AGG | 0.644801 | 3:+22970661 | MsG0380012702.01.T01:CDS |
| ATCTGGTAGACAAAACTGGA+CGG | 0.647904 | 3:+22970741 | MsG0380012702.01.T01:CDS |
| TGTTATGGCCACCTCCAGAG+TGG | 0.665853 | 3:+22971182 | MsG0380012702.01.T01:CDS |
| TTCAGCCACTAACTGGACAG+TGG | 0.686028 | 3:-22970789 | None:intergenic |
| AAACCAGTACTGTTGAAATG+CGG | 0.710188 | 3:+22970644 | MsG0380012702.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTGAATATTCTAAATTGTTA+TGG | + | Chr3:22971167-22971186 | MsG0380012702.01.T01:CDS | 20.0% |
| !!! | CATAACAATTTAGAATATTC+AGG | - | Chr3:22971169-22971188 | None:intergenic | 20.0% |
| !!! | TTTAGAATATTCAGGATATT+TGG | - | Chr3:22971161-22971180 | None:intergenic | 20.0% |
| ! | CAAGTATTAATTTCTTGGTA+CGG | + | Chr3:22970918-22970937 | MsG0380012702.01.T01:CDS | 25.0% |
| ! | GATAATATATCCACTTTATG+TGG | + | Chr3:22971091-22971110 | MsG0380012702.01.T01:CDS | 25.0% |
| ! | TCTTGCAAGTATTAATTTCT+TGG | + | Chr3:22970913-22970932 | MsG0380012702.01.T01:CDS | 25.0% |
| ! | TGTGTTTAAGCTTAATGAAA+AGG | + | Chr3:22970946-22970965 | MsG0380012702.01.T01:CDS | 25.0% |
| GACAAAAAACCTATCATACT+CGG | + | Chr3:22970596-22970615 | MsG0380012702.01.T01:CDS | 30.0% | |
| TAATGAAAAGGAGAAGAAGT+GGG | + | Chr3:22970958-22970977 | MsG0380012702.01.T01:CDS | 30.0% | |
| TTAATGAAAAGGAGAAGAAG+TGG | + | Chr3:22970957-22970976 | MsG0380012702.01.T01:CDS | 30.0% | |
| !!! | TTTATGTGGAAGCTGTTTTT+TGG | + | Chr3:22971105-22971124 | MsG0380012702.01.T01:CDS | 30.0% |
| AAAACAGCTTCCACATAAAG+TGG | - | Chr3:22971104-22971123 | None:intergenic | 35.0% | |
| AAACCAGTACTGTTGAAATG+CGG | + | Chr3:22970644-22970663 | MsG0380012702.01.T01:CDS | 35.0% | |
| AGGGAATTGTGTGATCATTA+TGG | + | Chr3:22971069-22971088 | MsG0380012702.01.T01:CDS | 35.0% | |
| ! | CAGATTTGTGTGTTGCTAAA+GGG | + | Chr3:22971050-22971069 | MsG0380012702.01.T01:CDS | 35.0% |
| ! | TCAGATTTGTGTGTTGCTAA+AGG | + | Chr3:22971049-22971068 | MsG0380012702.01.T01:CDS | 35.0% |
| !! | TATGAGTCTTTAGAGTTCGA+TGG | + | Chr3:22970884-22970903 | MsG0380012702.01.T01:CDS | 35.0% |
| !!! | AGGGGATAGAGTTTTGTTTT+TGG | + | Chr3:22970997-22971016 | MsG0380012702.01.T01:CDS | 35.0% |
| !!! | GGGGATAGAGTTTTGTTTTT+GGG | + | Chr3:22970998-22971017 | MsG0380012702.01.T01:CDS | 35.0% |
| !!! | TGTTTTTTGGACTTGGATGA+TGG | + | Chr3:22971118-22971137 | MsG0380012702.01.T01:CDS | 35.0% |
| !!! | TTTCAACAGTACTGGTTTTG+AGG | - | Chr3:22970642-22970661 | None:intergenic | 35.0% |
| AAAATTCTTGGTGGAGAGTG+AGG | + | Chr3:22970838-22970857 | MsG0380012702.01.T01:CDS | 40.0% | |
| AAATTCTTGGTGGAGAGTGA+GGG | + | Chr3:22970839-22970858 | MsG0380012702.01.T01:CDS | 40.0% | |
| ATCTGGTAGACAAAACTGGA+CGG | + | Chr3:22970741-22970760 | MsG0380012702.01.T01:CDS | 40.0% | |
| CCTTATCTGGTAGACAAAAC+TGG | + | Chr3:22970737-22970756 | MsG0380012702.01.T01:CDS | 40.0% | |
| CGGGAAAAGAAAATTCTTGG+TGG | + | Chr3:22970829-22970848 | MsG0380012702.01.T01:CDS | 40.0% | |
| TGAGGGTGAGTTATTGCTAA+CGG | + | Chr3:22970856-22970875 | MsG0380012702.01.T01:CDS | 40.0% | |
| TGGCGGGAAAAGAAAATTCT+TGG | + | Chr3:22970826-22970845 | MsG0380012702.01.T01:CDS | 40.0% | |
| ! | GAGACATCTGCCTTTTCAAT+GGG | + | Chr3:22970711-22970730 | MsG0380012702.01.T01:CDS | 40.0% |
| !! | CCAGTTTTGTCTACCAGATA+AGG | - | Chr3:22970740-22970759 | None:intergenic | 40.0% |
| !!! | ACAGTACTGGTTTTGAGGTA+CGG | - | Chr3:22970637-22970656 | None:intergenic | 40.0% |
| !!! | TGGAAGCTGTTTTTTGGACT+TGG | + | Chr3:22971111-22971130 | MsG0380012702.01.T01:CDS | 40.0% |
| GATAAGGCGACCCATTGAAA+AGG | - | Chr3:22970724-22970743 | None:intergenic | 45.0% | |
| GGGTGAAGTTGGCGAATTTA+GGG | + | Chr3:22970978-22970997 | MsG0380012702.01.T01:CDS | 45.0% | |
| GGTGAAGTTGGCGAATTTAG+GGG | + | Chr3:22970979-22970998 | MsG0380012702.01.T01:CDS | 45.0% | |
| TGAAATGCGGCGATGAGAAT+TGG | + | Chr3:22970657-22970676 | MsG0380012702.01.T01:CDS | 45.0% | |
| TGGGTGAAGTTGGCGAATTT+AGG | + | Chr3:22970977-22970996 | MsG0380012702.01.T01:CDS | 45.0% | |
| TTTCAATGGGTCGCCTTATC+TGG | + | Chr3:22970724-22970743 | MsG0380012702.01.T01:CDS | 45.0% | |
| ! | GGAGACATCTGCCTTTTCAA+TGG | + | Chr3:22970710-22970729 | MsG0380012702.01.T01:CDS | 45.0% |
| !!! | TGACAGCCACGACTTTTTCA+GGG | - | Chr3:22970568-22970587 | None:intergenic | 45.0% |
| ACGGACGGTTACTGTTAGAC+CGG | + | Chr3:22970760-22970779 | MsG0380012702.01.T01:CDS | 50.0% | |
| ATGCGGCGATGAGAATTGGA+AGG | + | Chr3:22970661-22970680 | MsG0380012702.01.T01:CDS | 50.0% | |
| CCAAAGGTTCAGCCACTAAC+TGG | - | Chr3:22970799-22970818 | None:intergenic | 50.0% | |
| CCAGTTAGTGGCTGAACCTT+TGG | + | Chr3:22970796-22970815 | MsG0380012702.01.T01:CDS | 50.0% | |
| CTAAGCGTGCCGAGTATGAT+AGG | - | Chr3:22970608-22970627 | None:intergenic | 50.0% | |
| GGAGAAGAAGTGGGTGAAGT+TGG | + | Chr3:22970967-22970986 | MsG0380012702.01.T01:CDS | 50.0% | |
| GGTAGACAAAACTGGACGGA+CGG | + | Chr3:22970745-22970764 | MsG0380012702.01.T01:CDS | 50.0% | |
| GTGGCTGAACCTTTGGTTGA+TGG | + | Chr3:22970803-22970822 | MsG0380012702.01.T01:CDS | 50.0% | |
| TCGCCGCATTTCAACAGTAC+TGG | - | Chr3:22970650-22970669 | None:intergenic | 50.0% | |
| TTCAGCCACTAACTGGACAG+TGG | - | Chr3:22970792-22970811 | None:intergenic | 50.0% | |
| ! | CTTCTTTTGGCGATCCACTC+TGG | - | Chr3:22971199-22971218 | None:intergenic | 50.0% |
| !! | AACCTTTGGTTGATGGTGGC+GGG | + | Chr3:22970810-22970829 | MsG0380012702.01.T01:CDS | 50.0% |
| !! | GAACCTTTGGTTGATGGTGG+CGG | + | Chr3:22970809-22970828 | MsG0380012702.01.T01:CDS | 50.0% |
| !! | GCTGAACCTTTGGTTGATGG+TGG | + | Chr3:22970806-22970825 | MsG0380012702.01.T01:CDS | 50.0% |
| !! | GTGACAGCCACGACTTTTTC+AGG | - | Chr3:22970569-22970588 | None:intergenic | 50.0% |
| AACTGGACAGTGGAGTCGTC+CGG | - | Chr3:22970782-22970801 | None:intergenic | 55.0% | |
| CGACTCCACTGTCCAGTTAG+TGG | + | Chr3:22970784-22970803 | MsG0380012702.01.T01:CDS | 55.0% | |
| TCCTGACATGTCGCCTTGCT+CGG | + | Chr3:22970688-22970707 | MsG0380012702.01.T01:CDS | 55.0% | |
| TGTTATGGCCACCTCCAGAG+TGG | + | Chr3:22971182-22971201 | MsG0380012702.01.T01:CDS | 55.0% | |
| TTCCCGCCACCATCAACCAA+AGG | - | Chr3:22970815-22970834 | None:intergenic | 55.0% | |
| ! | CTTTTGGCGATCCACTCTGG+AGG | - | Chr3:22971196-22971215 | None:intergenic | 55.0% |
| AGGCAGATGTCTCCCGAGCA+AGG | - | Chr3:22970704-22970723 | None:intergenic | 60.0% | |
| CCTGACATGTCGCCTTGCTC+GGG | + | Chr3:22970689-22970708 | MsG0380012702.01.T01:CDS | 60.0% | |
| ! | TTGGCGATCCACTCTGGAGG+TGG | - | Chr3:22971193-22971212 | None:intergenic | 60.0% |
| CCCGAGCAAGGCGACATGTC+AGG | - | Chr3:22970692-22970711 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 22970560 | 22971222 | 22970560 | ID=MsG0380012702.01;Name=MsG0380012702.01 |
| Chr3 | mRNA | 22970560 | 22971222 | 22970560 | ID=MsG0380012702.01.T01;Parent=MsG0380012702.01;Name=MsG0380012702.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|220 |
| Chr3 | exon | 22970560 | 22971222 | 22970560 | ID=MsG0380012702.01.T01:exon:8488;Parent=MsG0380012702.01.T01 |
| Chr3 | CDS | 22970560 | 22971222 | 22970560 | ID=MsG0380012702.01.T01:cds;Parent=MsG0380012702.01.T01 |
| Gene Sequence |
| Protein sequence |
