AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040476.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040476.01.T01 MTR_7g089010 95.724 304 5 4 1 301 1 299 0.0 575
MsG0780040476.01.T01 MTR_4g064730 44.292 219 65 4 5 182 8 210 4.30e-44 152
MsG0780040476.01.T01 MTR_4g121020 67.647 68 22 0 86 153 106 173 3.31e-25 105
MsG0780040476.01.T01 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.60e-25 105
MsG0780040476.01.T01 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.60e-25 105
MsG0780040476.01.T01 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.42e-22 98.2
MsG0780040476.01.T01 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.49e-22 98.2
MsG0780040476.01.T01 MTR_2g034960 60.294 68 27 0 86 153 206 273 5.54e-22 96.3
MsG0780040476.01.T01 MTR_3g106220 54.545 77 34 1 78 154 186 261 6.54e-22 96.3
MsG0780040476.01.T01 MTR_7g117705 63.636 66 21 1 90 155 190 252 3.61e-21 93.6
MsG0780040476.01.T01 MTR_1g032570 60.938 64 25 0 90 153 130 193 7.93e-21 92.8
MsG0780040476.01.T01 MTR_1g032570 60.938 64 25 0 90 153 199 262 9.43e-21 92.8
MsG0780040476.01.T01 MTR_1g013170 65.000 60 21 0 90 149 190 249 4.04e-20 90.5
MsG0780040476.01.T01 MTR_7g026400 34.559 136 75 3 82 217 148 269 5.52e-20 90.5
MsG0780040476.01.T01 MTR_4g131570 65.574 61 21 0 88 148 200 260 6.37e-20 90.1
MsG0780040476.01.T01 MTR_4g131600 54.412 68 31 0 82 149 192 259 2.33e-19 88.6
MsG0780040476.01.T01 MTR_0450s0040 52.857 70 33 0 88 157 166 235 4.52e-19 86.7
MsG0780040476.01.T01 MTR_1g013180 54.930 71 32 0 90 160 341 411 4.88e-19 87.8
MsG0780040476.01.T01 MTR_4g131580 65.574 61 21 0 88 148 197 257 4.96e-19 87.8
MsG0780040476.01.T01 MTR_3g102600 54.545 66 28 1 90 155 82 145 4.41e-18 80.5
MsG0780040476.01.T01 MTR_3g102600 54.545 66 28 1 90 155 131 194 7.29e-18 81.3
MsG0780040476.01.T01 MTR_8g079940 56.164 73 28 1 89 157 207 279 2.66e-17 82.4
MsG0780040476.01.T01 MTR_3g086100 51.389 72 35 0 90 161 127 198 7.16e-17 80.9
MsG0780040476.01.T01 MTR_3g086100 51.389 72 35 0 90 161 198 269 9.27e-17 80.9
MsG0780040476.01.T01 MTR_5g014040 56.667 60 26 0 90 149 310 369 1.66e-16 80.1
MsG0780040476.01.T01 MTR_4g098870 55.000 60 27 0 90 149 309 368 7.37e-16 78.2
MsG0780040476.01.T01 MTR_4g098870 58.929 56 23 0 90 145 309 364 7.69e-16 78.2
MsG0780040476.01.T01 MTR_4g098870 58.929 56 23 0 90 145 329 384 8.30e-16 78.2
MsG0780040476.01.T01 MTR_4g098870 55.000 60 27 0 90 149 329 388 8.49e-16 77.8
MsG0780040476.01.T01 MTR_4g098870 55.000 60 27 0 90 149 348 407 9.14e-16 77.8
MsG0780040476.01.T01 MTR_4g086835 51.515 66 32 0 84 149 267 332 1.06e-15 77.4
MsG0780040476.01.T01 MTR_4g021760 56.897 58 25 0 90 147 168 225 1.82e-15 75.9
MsG0780040476.01.T01 MTR_4g078810 51.667 60 29 0 90 149 157 216 2.28e-15 76.3
MsG0780040476.01.T01 MTR_2g090675 33.566 143 59 4 90 219 160 279 2.34e-15 76.3
MsG0780040476.01.T01 MTR_3g102590 44.304 79 42 1 90 166 142 220 2.94e-15 74.3
MsG0780040476.01.T01 MTR_5g014040 52.308 65 26 1 90 149 270 334 1.70e-14 73.9
MsG0780040476.01.T01 MTR_4g086835 50.746 67 32 1 84 149 267 333 1.82e-14 73.6
MsG0780040476.01.T01 MTR_5g014040 52.308 65 26 1 90 149 310 374 1.96e-14 73.9
MsG0780040476.01.T01 MTR_5g017980 52.542 59 28 0 90 148 191 249 4.70e-14 72.0
MsG0780040476.01.T01 MTR_5g017980 52.542 59 28 0 90 148 192 250 4.83e-14 71.6
MsG0780040476.01.T01 MTR_4g021790 54.237 59 27 0 90 148 167 225 6.95e-14 70.9
MsG0780040476.01.T01 MTR_4g113140 48.571 70 35 1 80 148 224 293 8.06e-14 71.6
MsG0780040476.01.T01 MTR_8g105600 57.895 57 24 0 94 150 190 246 1.13e-13 71.6
MsG0780040476.01.T01 MTR_5g041350 56.364 55 23 1 91 144 15 69 1.79e-13 70.5
MsG0780040476.01.T01 MTR_2g086450 55.385 65 27 2 91 154 45 108 4.28e-13 69.3
MsG0780040476.01.T01 MTR_5g017980 51.667 60 28 1 90 148 192 251 1.21e-12 67.8
MsG0780040476.01.T01 MTR_6g032990 40.000 95 45 3 91 181 42 128 1.22e-12 68.2
MsG0780040476.01.T01 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.43e-12 67.8
MsG0780040476.01.T01 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.50e-12 67.8
MsG0780040476.01.T01 MTR_1g090670 46.429 84 41 2 81 160 31 114 1.54e-12 67.0
MsG0780040476.01.T01 MTR_2g027800 50.685 73 35 1 91 162 39 111 1.73e-12 67.0
MsG0780040476.01.T01 MTR_4g021855 52.542 59 28 0 90 148 167 225 1.90e-12 67.0
MsG0780040476.01.T01 MTR_1g093080 50.000 60 29 1 90 148 210 269 1.98e-12 67.4
MsG0780040476.01.T01 MTR_7g098250 50.725 69 31 2 91 158 262 328 2.18e-12 67.8
MsG0780040476.01.T01 MTR_5g054300 51.667 60 28 1 90 148 197 256 3.03e-12 66.6
MsG0780040476.01.T01 MTR_1g053830 44.928 69 37 1 77 144 4 72 3.23e-12 62.4
MsG0780040476.01.T01 MTR_5g027440 56.364 55 23 1 91 144 25 79 3.83e-12 65.9
MsG0780040476.01.T01 MTR_3g102600 47.170 53 28 0 90 142 82 134 4.77e-12 62.8
MsG0780040476.01.T01 MTR_2g027800 60.000 55 21 1 91 144 39 93 5.56e-12 65.5
MsG0780040476.01.T01 MTR_3g102600 47.170 53 28 0 90 142 131 183 7.20e-12 63.5
MsG0780040476.01.T01 MTR_1g080330 55.556 54 23 1 91 143 175 228 9.41e-12 65.5
MsG0780040476.01.T01 MTR_1g080330 55.556 54 23 1 91 143 175 228 1.01e-11 64.7
MsG0780040476.01.T01 MTR_6g045327 48.276 58 30 0 90 147 171 228 1.09e-11 65.1
MsG0780040476.01.T01 MTR_4g081710 52.727 55 25 1 91 144 17 71 1.70e-11 64.3
MsG0780040476.01.T01 MTR_2g027860 58.182 55 22 1 91 144 49 103 1.86e-11 64.3
MsG0780040476.01.T01 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.21e-11 63.9
MsG0780040476.01.T01 MTR_8g066310 41.905 105 54 4 43 143 125 226 2.77e-11 63.9
MsG0780040476.01.T01 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.84e-11 63.5
MsG0780040476.01.T01 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.54e-11 63.9
MsG0780040476.01.T01 MTR_7g088070 42.857 105 46 4 78 170 37 139 3.60e-11 62.8
MsG0780040476.01.T01 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.88e-11 63.5
MsG0780040476.01.T01 MTR_0223s0040 56.364 55 23 1 90 143 17 71 8.74e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040476.01.T01 AT3G10760 54.571 350 95 10 1 301 1 335 2.34e-91 275
MsG0780040476.01.T01 AT5G05090 54.369 309 90 10 1 301 1 266 1.94e-80 244
MsG0780040476.01.T01 AT2G40970 61.682 214 57 6 1 199 1 204 1.27e-65 206
MsG0780040476.01.T01 AT3G46640 37.736 318 132 7 5 270 15 318 2.01e-52 175
MsG0780040476.01.T01 AT3G46640 37.736 318 132 7 5 270 15 318 2.01e-52 175
MsG0780040476.01.T01 AT3G46640 38.558 319 113 8 5 278 15 295 2.53e-52 174
MsG0780040476.01.T01 AT5G59570 45.933 209 66 4 11 184 28 224 4.79e-45 155
MsG0780040476.01.T01 AT5G59570 45.933 209 66 4 11 184 28 224 4.79e-45 155
MsG0780040476.01.T01 AT3G16857 60.000 75 30 0 79 153 155 229 4.48e-24 102
MsG0780040476.01.T01 AT3G16857 60.000 75 30 0 79 153 225 299 5.35e-24 102
MsG0780040476.01.T01 AT3G16857 60.000 75 30 0 79 153 225 299 6.00e-24 102
MsG0780040476.01.T01 AT5G58080 66.129 62 21 0 88 149 191 252 5.72e-22 96.3
MsG0780040476.01.T01 AT2G01760 63.077 65 24 0 90 154 134 198 3.01e-21 92.0
MsG0780040476.01.T01 AT2G01760 63.077 65 24 0 90 154 199 263 7.56e-21 92.0
MsG0780040476.01.T01 AT2G25180 61.538 65 25 0 90 154 194 258 9.74e-21 92.8
MsG0780040476.01.T01 AT2G01760 63.077 65 24 0 90 154 227 291 1.23e-20 91.7
MsG0780040476.01.T01 AT4G31920 61.667 60 23 0 90 149 182 241 4.07e-19 87.8
MsG0780040476.01.T01 AT5G07210 50.667 75 35 1 82 154 221 295 3.50e-17 82.4
MsG0780040476.01.T01 AT5G07210 50.667 75 35 1 82 154 213 287 3.60e-17 82.0
MsG0780040476.01.T01 AT3G62670 38.710 124 66 4 90 205 210 331 4.87e-16 78.6
MsG0780040476.01.T01 AT5G44190 55.000 60 27 0 90 149 146 205 5.50e-16 78.2
MsG0780040476.01.T01 AT5G44190 55.000 60 27 0 90 149 146 205 6.42e-16 77.4
MsG0780040476.01.T01 AT2G20570 55.000 60 27 0 90 149 152 211 3.94e-15 75.9
MsG0780040476.01.T01 AT2G20570 57.895 57 24 0 93 149 171 227 9.21e-15 74.7
MsG0780040476.01.T01 AT1G67710 59.322 59 23 1 93 150 121 179 2.01e-14 73.6
MsG0780040476.01.T01 AT1G67710 59.322 59 23 1 93 150 130 188 2.19e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT4G18020 54.237 59 27 0 91 149 296 354 2.47e-14 73.6
MsG0780040476.01.T01 AT1G67710 59.322 59 23 1 93 150 195 253 3.03e-14 73.2
MsG0780040476.01.T01 AT3G12730 61.818 55 20 1 91 144 23 77 3.06e-14 71.2
MsG0780040476.01.T01 AT2G27070 48.529 68 35 0 82 149 216 283 3.75e-14 73.2
MsG0780040476.01.T01 AT3G04030 57.407 54 23 0 91 144 45 98 9.88e-14 71.2
MsG0780040476.01.T01 AT5G45580 58.182 55 22 1 91 144 7 61 2.42e-13 69.3
MsG0780040476.01.T01 AT1G79430 61.818 55 20 1 91 144 34 88 2.64e-13 70.1
MsG0780040476.01.T01 AT5G45580 58.182 55 22 1 91 144 23 77 2.82e-13 69.3
MsG0780040476.01.T01 AT2G03500 53.333 60 27 1 90 148 232 291 3.40e-13 70.1
MsG0780040476.01.T01 AT3G04030 58.182 55 22 1 91 144 45 99 4.86e-13 69.3
MsG0780040476.01.T01 AT3G04030 58.182 55 22 1 91 144 45 99 5.39e-13 69.3
MsG0780040476.01.T01 AT3G24120 60.000 55 21 1 91 144 41 95 6.05e-13 68.6
MsG0780040476.01.T01 AT3G24120 60.000 55 21 1 91 144 41 95 6.19e-13 68.2
MsG0780040476.01.T01 AT4G13640 60.000 55 21 1 91 144 37 91 6.87e-13 68.2
MsG0780040476.01.T01 AT4G13640 60.000 55 21 1 91 144 37 91 7.23e-13 68.2
MsG0780040476.01.T01 AT5G18240 60.000 55 21 1 91 144 45 99 9.46e-13 68.6
MsG0780040476.01.T01 AT5G18240 60.000 55 21 1 91 144 45 99 9.46e-13 68.6
MsG0780040476.01.T01 AT5G49240 52.727 55 26 0 86 140 218 272 9.90e-13 67.8
MsG0780040476.01.T01 AT5G18240 60.000 55 21 1 91 144 45 99 1.08e-12 68.2
MsG0780040476.01.T01 AT5G18240 60.000 55 21 1 91 144 45 99 1.09e-12 68.2
MsG0780040476.01.T01 AT5G18240 60.000 55 21 1 91 144 45 99 1.09e-12 68.2
MsG0780040476.01.T01 AT1G13300 51.667 60 28 1 90 148 180 239 1.15e-12 68.2
MsG0780040476.01.T01 AT1G25550 51.667 60 28 1 90 148 208 267 2.22e-12 67.0
MsG0780040476.01.T01 AT1G49190 32.692 104 62 4 90 187 214 315 9.20e-12 65.5
MsG0780040476.01.T01 AT2G01060 58.182 55 22 1 91 144 15 69 1.29e-11 64.3
MsG0780040476.01.T01 AT1G68670 50.000 62 30 1 88 148 212 273 1.96e-11 64.3
MsG0780040476.01.T01 AT4G37180 50.000 60 29 1 90 148 209 268 3.60e-11 63.5
MsG0780040476.01.T01 AT1G49560 48.387 62 31 1 88 148 189 250 3.71e-11 63.5
MsG0780040476.01.T01 AT4G37180 50.000 60 29 1 90 148 216 275 4.24e-11 63.5
MsG0780040476.01.T01 AT3G13040 53.571 56 25 1 90 144 240 295 4.76e-11 63.5
MsG0780040476.01.T01 AT3G13040 53.571 56 25 1 90 144 240 295 4.76e-11 63.5
MsG0780040476.01.T01 AT3G13040 53.571 56 25 1 90 144 240 295 4.76e-11 63.5

Find 97 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGTCCGGTTGGACATTT+TGG 0.084147 7:+78157417 MsG0780040476.01.T01:CDS
GGGATAGTGGTGGGTGGATT+AGG 0.144441 7:-78156901 None:intergenic
CAGCAGCAGCTTCATAGAAT+TGG 0.261093 7:+78157525 MsG0780040476.01.T01:CDS
GTGATTAGGGTTTGTGATAA+AGG 0.272510 7:-78156841 None:intergenic
GGATAGTGGTGGGTGGATTA+GGG 0.278062 7:-78156900 None:intergenic
TGAGAGAAGATGATTCAAAT+TGG 0.306698 7:+78156773 MsG0780040476.01.T01:CDS
TGGCGAGATCAGGTGTGATT+AGG 0.327778 7:-78156855 None:intergenic
TCCGGTTGCGCGGCCGAATT+CGG 0.334342 7:+78157332 MsG0780040476.01.T01:CDS
TTCAGTTGAATCGGCGAAAT+CGG 0.335433 7:-78156974 None:intergenic
TGGTGGTTATGTGGAGGATA+TGG 0.336921 7:+78157593 MsG0780040476.01.T01:CDS
AGCAGCAGCTTCATAGAATT+GGG 0.346797 7:+78157526 MsG0780040476.01.T01:CDS
CGCATGCAGGGTATTTCTTC+CGG 0.354356 7:+78157213 MsG0780040476.01.T01:CDS
GGCGAGATCAGGTGTGATTA+GGG 0.364114 7:-78156854 None:intergenic
GTGGAGGAAGGAAGAGAAGA+AGG 0.372281 7:-78156952 None:intergenic
CGGCGTCGACGAAGCGTTTG+TGG 0.387001 7:-78157071 None:intergenic
TTGTTGTGGTGGTGGGATAG+TGG 0.396749 7:-78156914 None:intergenic
TCCGAATTCGGCCGCGCAAC+CGG 0.399183 7:-78157333 None:intergenic
CAATTCTATGAAGCTGCTGC+TGG 0.402280 7:-78157524 None:intergenic
GGTGGTGGTGGTTCCGGTGG+TGG 0.402979 7:+78157237 MsG0780040476.01.T01:CDS
GATAAAGGCATGAGTTCTTC+TGG 0.404291 7:-78156826 None:intergenic
TGGTTCTTCGCCGGCGGTGC+CGG 0.408437 7:-78157004 None:intergenic
TGAAGAAAATGCGGCGGTAC+AGG 0.409052 7:-78157309 None:intergenic
GGCATAAAATGATCCGAATT+CGG 0.417026 7:-78157345 None:intergenic
ACCGGAACCACCACCACCAC+CGG 0.421482 7:-78157232 None:intergenic
TATGAAGCTGCTGCTGGTGC+TGG 0.424890 7:-78157518 None:intergenic
TCGAAAGCGATGGCGAGATC+AGG 0.427672 7:-78156865 None:intergenic
ACTTTAGGTGGTGGTGGTGG+TGG 0.427931 7:+78157567 MsG0780040476.01.T01:CDS
GTTGTTGTTGTTGTGGTGGT+GGG 0.428602 7:-78156921 None:intergenic
TGGAGGAAGGAAGAGAAGAA+GGG 0.433177 7:-78156951 None:intergenic
CAGGTGCATCATAACACTTT+AGG 0.433744 7:+78157552 MsG0780040476.01.T01:CDS
CATAAGCTGCATTATCGTCT+TGG 0.434900 7:-78157124 None:intergenic
ATTTCTTCCGGTGGTGGTGG+TGG 0.438743 7:+78157225 MsG0780040476.01.T01:CDS
TTGTGGTGGTGGGATAGTGG+TGG 0.448450 7:-78156911 None:intergenic
TCAAACGCCCTCGTCTTGTA+TGG 0.450475 7:+78157037 MsG0780040476.01.T01:CDS
TCTTTATCTTAAACGCATGC+AGG 0.456959 7:+78157200 MsG0780040476.01.T01:CDS
CAGGGTATTTCTTCCGGTGG+TGG 0.460767 7:+78157219 MsG0780040476.01.T01:CDS
GGTATTTCTTCCGGTGGTGG+TGG 0.460989 7:+78157222 MsG0780040476.01.T01:CDS
CGAAGCGTTTGTGGAGCTGT+GGG 0.467151 7:-78157062 None:intergenic
GTTCTGACTTTGTTTCCAAC+CGG 0.469745 7:+78157645 MsG0780040476.01.T01:CDS
GTCGACGCCGTGGCGCATCT+CGG 0.482986 7:+78157084 MsG0780040476.01.T01:CDS
ATGGAATCAGCTAATGCAAC+CGG 0.487862 7:+78157612 MsG0780040476.01.T01:CDS
ATAAGCTGCATTATCGTCTT+GGG 0.488899 7:-78157123 None:intergenic
AACCCAAATTCAATCATCTC+CGG 0.488992 7:-78157664 None:intergenic
TGATGATGAATAGCAGGAAC+AGG 0.492936 7:-78157375 None:intergenic
AACACTTTAGGTGGTGGTGG+TGG 0.493430 7:+78157564 MsG0780040476.01.T01:CDS
GGATTATCAAAATGTCCATT+CGG 0.494489 7:-78157447 None:intergenic
GAGGAAGGAAGAGAAGAAGG+GGG 0.497445 7:-78156949 None:intergenic
GGCATGAGTTCTTCTGGTGG+TGG 0.507634 7:-78156820 None:intergenic
GGCGACCCAAAATGTCCAAC+CGG 0.507671 7:-78157423 None:intergenic
TGCTGCTGATGATGAATAGC+AGG 0.508146 7:-78157381 None:intergenic
TAATGCAACCGGAGGGAGAA+AGG 0.508295 7:+78157623 MsG0780040476.01.T01:CDS
GGAGGTGAGTTCAGTTGAAT+CGG 0.513901 7:-78156983 None:intergenic
GGAGGAAGGAAGAGAAGAAG+GGG 0.527059 7:-78156950 None:intergenic
AGTCAGAACCTTTCTCCCTC+CGG 0.527350 7:-78157631 None:intergenic
ACGAAGCGTTTGTGGAGCTG+TGG 0.532297 7:-78157063 None:intergenic
TGAATCGGCGAAATCGGTGG+AGG 0.543830 7:-78156968 None:intergenic
TTCTTCGCCGGCGGTGCCGG+AGG 0.545793 7:-78157001 None:intergenic
GAACTCACCTCCGGCACCGC+CGG 0.547744 7:+78156994 MsG0780040476.01.T01:CDS
TGTGGTGGTGGGATAGTGGT+GGG 0.548736 7:-78156910 None:intergenic
GCTTCATAGAATTGGGGCAC+AGG 0.550758 7:+78157533 MsG0780040476.01.T01:CDS
CATAACACTTTAGGTGGTGG+TGG 0.551977 7:+78157561 MsG0780040476.01.T01:CDS
GATGGCTCAGTATCATCGTC+CGG 0.554969 7:+78157404 MsG0780040476.01.T01:CDS
AGTTGAATCGGCGAAATCGG+TGG 0.557119 7:-78156971 None:intergenic
GAAGAAAATGCGGCGGTACA+GGG 0.558776 7:-78157308 None:intergenic
GGGCGTTTGAGTGTGCGAGC+TGG 0.560084 7:-78157024 None:intergenic
CTTTATCTTAAACGCATGCA+GGG 0.564649 7:+78157201 MsG0780040476.01.T01:CDS
TGTGCGAGCTGGTTCTTCGC+CGG 0.569228 7:-78157013 None:intergenic
TCGGCGAAATCGGTGGAGGA+AGG 0.569986 7:-78156964 None:intergenic
ATAGCAGGAACAGGAACAAA+CGG 0.570737 7:-78157366 None:intergenic
CATCATAACACTTTAGGTGG+TGG 0.574928 7:+78157558 MsG0780040476.01.T01:CDS
AACAGAAGCAGAACCACCAC+CGG 0.574953 7:-78157250 None:intergenic
GCATGAGTTCTTCTGGTGGT+GGG 0.580278 7:-78156819 None:intergenic
GCTCAGTATCATCGTCCGGT+TGG 0.583168 7:+78157408 MsG0780040476.01.T01:CDS
GTTTCGTATGTCGAAAGCGA+TGG 0.584109 7:-78156875 None:intergenic
AAGAAAATGCGGCGGTACAG+GGG 0.587168 7:-78157307 None:intergenic
ATGCAGGGTATTTCTTCCGG+TGG 0.591915 7:+78157216 MsG0780040476.01.T01:CDS
CAAATTCAATCATCTCCGGT+TGG 0.598529 7:-78157660 None:intergenic
GGTGGTGGGATAGTGGTGGG+TGG 0.599530 7:-78156907 None:intergenic
ATGCAGCTTATGAGTGTCGA+CGG 0.600961 7:+78157135 MsG0780040476.01.T01:CDS
TATTCATCATCAGCAGCAGA+TGG 0.603490 7:+78157386 MsG0780040476.01.T01:CDS
GCGAGCTGGTTCTTCGCCGG+CGG 0.605171 7:-78157010 None:intergenic
TGGTGGTGGTGGTTATGTGG+AGG 0.625699 7:+78157587 MsG0780040476.01.T01:CDS
CGGTTTAACGAGAGAAAACG+TGG 0.626632 7:+78157155 MsG0780040476.01.T01:CDS
CTTGATACCGAGATGCGCCA+CGG 0.632900 7:-78157091 None:intergenic
GAATCAGCTAATGCAACCGG+AGG 0.639788 7:+78157615 MsG0780040476.01.T01:CDS
GATTCAACTGAACTCACCTC+CGG 0.640353 7:+78156985 MsG0780040476.01.T01:CDS
GCAGCAGCTTCATAGAATTG+GGG 0.666624 7:+78157527 MsG0780040476.01.T01:CDS
GTGCATCATAACACTTTAGG+TGG 0.672948 7:+78157555 MsG0780040476.01.T01:CDS
TGTGGGGTCCATACAAGACG+AGG 0.673250 7:-78157045 None:intergenic
CAAACGCTTCGTCGACGCCG+TGG 0.674680 7:+78157074 MsG0780040476.01.T01:CDS
ACCGGATGAAGAAAATGCGG+CGG 0.695667 7:-78157315 None:intergenic
GAAGCGTTTGTGGAGCTGTG+GGG 0.695777 7:-78157061 None:intergenic
TTATCAAAATGTCCATTCGG+CGG 0.700387 7:-78157444 None:intergenic
AATCAGCTAATGCAACCGGA+GGG 0.707357 7:+78157616 MsG0780040476.01.T01:CDS
GTGGGGTCCATACAAGACGA+GGG 0.708697 7:-78157044 None:intergenic
GCAACCGGATGAAGAAAATG+CGG 0.719489 7:-78157318 None:intergenic
AAAGGCATGAGTTCTTCTGG+TGG 0.761512 7:-78156823 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAAATTGGTTTTCAAAA+TGG + Chr7:78156785-78156804 MsG0780040476.01.T01:CDS 20.0%
!!! TTCAAATTGGTTTTCAAAAT+GGG + Chr7:78156786-78156805 MsG0780040476.01.T01:CDS 20.0%
GGATTATCAAAATGTCCATT+CGG - Chr7:78157450-78157469 None:intergenic 30.0%
TGAGAGAAGATGATTCAAAT+TGG + Chr7:78156773-78156792 MsG0780040476.01.T01:CDS 30.0%
ATAAGCTGCATTATCGTCTT+GGG - Chr7:78157126-78157145 None:intergenic 35.0%
CTTTATCTTAAACGCATGCA+GGG + Chr7:78157201-78157220 MsG0780040476.01.T01:CDS 35.0%
GGCATAAAATGATCCGAATT+CGG - Chr7:78157348-78157367 None:intergenic 35.0%
GTGATTAGGGTTTGTGATAA+AGG - Chr7:78156844-78156863 None:intergenic 35.0%
TCTTTATCTTAAACGCATGC+AGG + Chr7:78157200-78157219 MsG0780040476.01.T01:CDS 35.0%
TGTTGCTGTCTAGACAAAAA+AGG - Chr7:78157471-78157490 None:intergenic 35.0%
TTATCAAAATGTCCATTCGG+CGG - Chr7:78157447-78157466 None:intergenic 35.0%
AGCAGCAGCTTCATAGAATT+GGG + Chr7:78157526-78157545 MsG0780040476.01.T01:CDS 40.0%
ATAGCAGGAACAGGAACAAA+CGG - Chr7:78157369-78157388 None:intergenic 40.0%
ATGGAATCAGCTAATGCAAC+CGG + Chr7:78157612-78157631 MsG0780040476.01.T01:CDS 40.0%
CAGGTGCATCATAACACTTT+AGG + Chr7:78157552-78157571 MsG0780040476.01.T01:CDS 40.0%
CATAAGCTGCATTATCGTCT+TGG - Chr7:78157127-78157146 None:intergenic 40.0%
CATCATAACACTTTAGGTGG+TGG + Chr7:78157558-78157577 MsG0780040476.01.T01:CDS 40.0%
GTGCATCATAACACTTTAGG+TGG + Chr7:78157555-78157574 MsG0780040476.01.T01:CDS 40.0%
TATTCATCATCAGCAGCAGA+TGG + Chr7:78157386-78157405 MsG0780040476.01.T01:CDS 40.0%
TTCAGTTGAATCGGCGAAAT+CGG - Chr7:78156977-78156996 None:intergenic 40.0%
! GATAAAGGCATGAGTTCTTC+TGG - Chr7:78156829-78156848 None:intergenic 40.0%
! GTTCTGACTTTGTTTCCAAC+CGG + Chr7:78157645-78157664 MsG0780040476.01.T01:CDS 40.0%
! TGATGATGAATAGCAGGAAC+AGG - Chr7:78157378-78157397 None:intergenic 40.0%
AATCAGCTAATGCAACCGGA+GGG + Chr7:78157616-78157635 MsG0780040476.01.T01:CDS 45.0%
CAATTCTATGAAGCTGCTGC+TGG - Chr7:78157527-78157546 None:intergenic 45.0%
CAGCAGCAGCTTCATAGAAT+TGG + Chr7:78157525-78157544 MsG0780040476.01.T01:CDS 45.0%
CGGTTTAACGAGAGAAAACG+TGG + Chr7:78157155-78157174 MsG0780040476.01.T01:CDS 45.0%
GATTCAACTGAACTCACCTC+CGG + Chr7:78156985-78157004 MsG0780040476.01.T01:CDS 45.0%
GCAACCGGATGAAGAAAATG+CGG - Chr7:78157321-78157340 None:intergenic 45.0%
GCAGCAGCTTCATAGAATTG+GGG + Chr7:78157527-78157546 MsG0780040476.01.T01:CDS 45.0%
GGAGGTGAGTTCAGTTGAAT+CGG - Chr7:78156986-78157005 None:intergenic 45.0%
GTTTCGTATGTCGAAAGCGA+TGG - Chr7:78156878-78156897 None:intergenic 45.0%
TGGAGGAAGGAAGAGAAGAA+GGG - Chr7:78156954-78156973 None:intergenic 45.0%
TGGTGGTTATGTGGAGGATA+TGG + Chr7:78157593-78157612 MsG0780040476.01.T01:CDS 45.0%
! AAAGGCATGAGTTCTTCTGG+TGG - Chr7:78156826-78156845 None:intergenic 45.0%
! ATCGTCCGGTTGGACATTTT+GGG + Chr7:78157418-78157437 MsG0780040476.01.T01:CDS 45.0%
! ATTTTCTTCATCCGGTTGCG+CGG + Chr7:78157322-78157341 MsG0780040476.01.T01:CDS 45.0%
! CATAACACTTTAGGTGGTGG+TGG + Chr7:78157561-78157580 MsG0780040476.01.T01:CDS 45.0%
! GGTGGTTGTTGTTGTTGTTG+TGG - Chr7:78156931-78156950 None:intergenic 45.0%
! GGTTGTTGTTGTTGTTGTGG+TGG - Chr7:78156928-78156947 None:intergenic 45.0%
! GTTGTTGTTGTTGTGGTGGT+GGG - Chr7:78156924-78156943 None:intergenic 45.0%
! TGCTGCTGATGATGAATAGC+AGG - Chr7:78157384-78157403 None:intergenic 45.0%
! TGTTGTTGTTGTTGTGGTGG+TGG - Chr7:78156925-78156944 None:intergenic 45.0%
!! ATGCAGCTTATGAGTGTCGA+CGG + Chr7:78157135-78157154 MsG0780040476.01.T01:CDS 45.0%
AACAGAAGCAGAACCACCAC+CGG - Chr7:78157253-78157272 None:intergenic 50.0%
AAGAAAATGCGGCGGTACAG+GGG - Chr7:78157310-78157329 None:intergenic 50.0%
ACCGGATGAAGAAAATGCGG+CGG - Chr7:78157318-78157337 None:intergenic 50.0%
AGTCAGAACCTTTCTCCCTC+CGG - Chr7:78157634-78157653 None:intergenic 50.0%
AGTTGAATCGGCGAAATCGG+TGG - Chr7:78156974-78156993 None:intergenic 50.0%
GAAGAAAATGCGGCGGTACA+GGG - Chr7:78157311-78157330 None:intergenic 50.0%
GAATCAGCTAATGCAACCGG+AGG + Chr7:78157615-78157634 MsG0780040476.01.T01:CDS 50.0%
GAGGAAGGAAGAGAAGAAGG+GGG - Chr7:78156952-78156971 None:intergenic 50.0%
GATGGCTCAGTATCATCGTC+CGG + Chr7:78157404-78157423 MsG0780040476.01.T01:CDS 50.0%
GCATGAGTTCTTCTGGTGGT+GGG - Chr7:78156822-78156841 None:intergenic 50.0%
GCTTCATAGAATTGGGGCAC+AGG + Chr7:78157533-78157552 MsG0780040476.01.T01:CDS 50.0%
GGAGGAAGGAAGAGAAGAAG+GGG - Chr7:78156953-78156972 None:intergenic 50.0%
GGCGAGATCAGGTGTGATTA+GGG - Chr7:78156857-78156876 None:intergenic 50.0%
GTGGAGGAAGGAAGAGAAGA+AGG - Chr7:78156955-78156974 None:intergenic 50.0%
TAATGCAACCGGAGGGAGAA+AGG + Chr7:78157623-78157642 MsG0780040476.01.T01:CDS 50.0%
TCAAACGCCCTCGTCTTGTA+TGG + Chr7:78157037-78157056 MsG0780040476.01.T01:CDS 50.0%
TGAAGAAAATGCGGCGGTAC+AGG - Chr7:78157312-78157331 None:intergenic 50.0%
TGGCGAGATCAGGTGTGATT+AGG - Chr7:78156858-78156877 None:intergenic 50.0%
! ACCGCCGCATTTTCTTCATC+CGG + Chr7:78157314-78157333 MsG0780040476.01.T01:CDS 50.0%
! ATGCAGGGTATTTCTTCCGG+TGG + Chr7:78157216-78157235 MsG0780040476.01.T01:CDS 50.0%
! CATCGTCCGGTTGGACATTT+TGG + Chr7:78157417-78157436 MsG0780040476.01.T01:CDS 50.0%
! CGCATGCAGGGTATTTCTTC+CGG + Chr7:78157213-78157232 MsG0780040476.01.T01:CDS 50.0%
!! AACACTTTAGGTGGTGGTGG+TGG + Chr7:78157564-78157583 MsG0780040476.01.T01:CDS 50.0%
!! GGATAGTGGTGGGTGGATTA+GGG - Chr7:78156903-78156922 None:intergenic 50.0%
!! TTGTTGTGGTGGTGGGATAG+TGG - Chr7:78156917-78156936 None:intergenic 50.0%
CTTGATACCGAGATGCGCCA+CGG - Chr7:78157094-78157113 None:intergenic 55.0%
GAAGGAAGAGAAGAAGGGGG+TGG - Chr7:78156949-78156968 None:intergenic 55.0%
GCTCAGTATCATCGTCCGGT+TGG + Chr7:78157408-78157427 MsG0780040476.01.T01:CDS 55.0%
GGCATGAGTTCTTCTGGTGG+TGG - Chr7:78156823-78156842 None:intergenic 55.0%
GGCGACCCAAAATGTCCAAC+CGG - Chr7:78157426-78157445 None:intergenic 55.0%
GTGGGGTCCATACAAGACGA+GGG - Chr7:78157047-78157066 None:intergenic 55.0%
TATGAAGCTGCTGCTGGTGC+TGG - Chr7:78157521-78157540 None:intergenic 55.0%
TCGAAAGCGATGGCGAGATC+AGG - Chr7:78156868-78156887 None:intergenic 55.0%
TGAATCGGCGAAATCGGTGG+AGG - Chr7:78156971-78156990 None:intergenic 55.0%
TGTGGGGTCCATACAAGACG+AGG - Chr7:78157048-78157067 None:intergenic 55.0%
! ACGAAGCGTTTGTGGAGCTG+TGG - Chr7:78157066-78157085 None:intergenic 55.0%
! CAGGGTATTTCTTCCGGTGG+TGG + Chr7:78157219-78157238 MsG0780040476.01.T01:CDS 55.0%
! CGAAGCGTTTGTGGAGCTGT+GGG - Chr7:78157065-78157084 None:intergenic 55.0%
! GAAGCGTTTGTGGAGCTGTG+GGG - Chr7:78157064-78157083 None:intergenic 55.0%
! GGTATTTCTTCCGGTGGTGG+TGG + Chr7:78157222-78157241 MsG0780040476.01.T01:CDS 55.0%
!! ACTTTAGGTGGTGGTGGTGG+TGG + Chr7:78157567-78157586 MsG0780040476.01.T01:CDS 55.0%
!! ATTTCTTCCGGTGGTGGTGG+TGG + Chr7:78157225-78157244 MsG0780040476.01.T01:CDS 55.0%
!! GGGATAGTGGTGGGTGGATT+AGG - Chr7:78156904-78156923 None:intergenic 55.0%
!! TGGTGGTGGTGGTGGTTATG+TGG + Chr7:78157584-78157603 MsG0780040476.01.T01:CDS 55.0%
!! TGGTGGTGGTGGTTATGTGG+AGG + Chr7:78157587-78157606 MsG0780040476.01.T01:CDS 55.0%
!! TGTGGTGGTGGGATAGTGGT+GGG - Chr7:78156913-78156932 None:intergenic 55.0%
!! TTGTGGTGGTGGGATAGTGG+TGG - Chr7:78156914-78156933 None:intergenic 55.0%
TCGGCGAAATCGGTGGAGGA+AGG - Chr7:78156967-78156986 None:intergenic 60.0%
! TGTGCGAGCTGGTTCTTCGC+CGG - Chr7:78157016-78157035 None:intergenic 60.0%
!! CATTTTGGGTCGCCGCCGAA+TGG + Chr7:78157432-78157451 MsG0780040476.01.T01:CDS 60.0%
!! TTAGGTGGTGGTGGTGGTGG+TGG + Chr7:78157570-78157589 MsG0780040476.01.T01:CDS 60.0%
ACCGGAACCACCACCACCAC+CGG - Chr7:78157235-78157254 None:intergenic 65.0%
CAAACGCTTCGTCGACGCCG+TGG + Chr7:78157074-78157093 MsG0780040476.01.T01:CDS 65.0%
GGGCGTTTGAGTGTGCGAGC+TGG - Chr7:78157027-78157046 None:intergenic 65.0%
TCCGAATTCGGCCGCGCAAC+CGG - Chr7:78157336-78157355 None:intergenic 65.0%
TCCGGTTGCGCGGCCGAATT+CGG + Chr7:78157332-78157351 MsG0780040476.01.T01:CDS 65.0%
!! CGGCGTCGACGAAGCGTTTG+TGG - Chr7:78157074-78157093 None:intergenic 65.0%
!! GGTGGTGGGATAGTGGTGGG+TGG - Chr7:78156910-78156929 None:intergenic 65.0%
!! TCCGGTGGTGGTGGTGGTTC+CGG + Chr7:78157231-78157250 MsG0780040476.01.T01:CDS 65.0%
GAACTCACCTCCGGCACCGC+CGG + Chr7:78156994-78157013 MsG0780040476.01.T01:CDS 70.0%
GTCGACGCCGTGGCGCATCT+CGG + Chr7:78157084-78157103 MsG0780040476.01.T01:CDS 70.0%
TGGTTCTTCGCCGGCGGTGC+CGG - Chr7:78157007-78157026 None:intergenic 70.0%
! GCGAGCTGGTTCTTCGCCGG+CGG - Chr7:78157013-78157032 None:intergenic 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG + Chr7:78157573-78157592 MsG0780040476.01.T01:CDS 70.0%
!! GGTGGTGGTGGTGGTTCCGG+TGG + Chr7:78157234-78157253 MsG0780040476.01.T01:CDS 70.0%
!! GGTGGTGGTGGTTCCGGTGG+TGG + Chr7:78157237-78157256 MsG0780040476.01.T01:CDS 70.0%
! TTCTTCGCCGGCGGTGCCGG+AGG - Chr7:78157004-78157023 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 78156772 78157677 78156772 ID=MsG0780040476.01;Name=MsG0780040476.01
Chr7 mRNA 78156772 78157677 78156772 ID=MsG0780040476.01.T01;Parent=MsG0780040476.01;Name=MsG0780040476.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|301
Chr7 exon 78156772 78157677 78156772 ID=MsG0780040476.01.T01:exon:15558;Parent=MsG0780040476.01.T01
Chr7 CDS 78156772 78157677 78156772 ID=MsG0780040476.01.T01:cds;Parent=MsG0780040476.01.T01
Gene Sequence

>MsG0780040476.01.T01

ATGAGAGAAGATGATTCAAATTGGTTTTCAAAATGGGAAGAACAACTCCCACCACCAGAAGAACTCATGCCTTTATCACAAACCCTAATCACACCTGATCTCGCCATCGCTTTCGACATACGAAACAACCCTAATCCACCCACCACTATCCCACCACCACAACAACAACAACAACCACCCCCTTCTTCTCTTCCTTCCTCCACCGATTTCGCCGATTCAACTGAACTCACCTCCGGCACCGCCGGCGAAGAACCAGCTCGCACACTCAAACGCCCTCGTCTTGTATGGACCCCACAGCTCCACAAACGCTTCGTCGACGCCGTGGCGCATCTCGGTATCAAGAACGCTGTTCCCAAGACGATAATGCAGCTTATGAGTGTCGACGGTTTAACGAGAGAAAACGTGGCGAGTCATCTTCAGAAATATCGTCTTTATCTTAAACGCATGCAGGGTATTTCTTCCGGTGGTGGTGGTGGTTCCGGTGGTGGTTCTGCTTCTGTTAATGATGCTGCTACTGATCATTTATTTGCTAGCTCCCCTGTACCGCCGCATTTTCTTCATCCGGTTGCGCGGCCGAATTCGGATCATTTTATGCCGTTTGTTCCTGTTCCTGCTATTCATCATCAGCAGCAGATGGCTCAGTATCATCGTCCGGTTGGACATTTTGGGTCGCCGCCGAATGGACATTTTGATAATCCTTTTTTGTCTAGACAGCAACATCATCAACAGCAAAATCAGCATCAACACCAGCACCAGCAGCAGCTTCATAGAATTGGGGCACAGGTGCATCATAACACTTTAGGTGGTGGTGGTGGTGGTGGTGGTGGTTATGTGGAGGATATGGAATCAGCTAATGCAACCGGAGGGAGAAAGGTTCTGACTTTGTTTCCAACCGGAGATGATTGA
Protein sequence

>MsG0780040476.01.T01

MREDDSNWFSKWEEQLPPPEELMPLSQTLITPDLAIAFDIRNNPNPPTTIPPPQQQQQPPPSSLPSSTDFADSTELTSGTAGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGISSGGGGGSGGGSASVNDAATDHLFASSPVPPHFLHPVARPNSDHFMPFVPVPAIHHQQQMAQYHRPVGHFGSPPNGHFDNPFLSRQQHHQQQNQHQHQHQQQLHRIGAQVHHNTLGGGGGGGGGYVEDMESANATGGRKVLTLFPTGDD*