Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046384.01.T01 | QSD99523.1 | 87.671 | 292 | 19 | 3 | 1 | 276 | 1 | 291 | 1.33E-163 | 465 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046384.01.T01 | Q2V9B0 | 55.634 | 284 | 97 | 9 | 14 | 276 | 22 | 297 | 5.08E-64 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046384.01.T01 | A0A896WBR5 | 87.671 | 292 | 19 | 3 | 1 | 276 | 1 | 291 | 6.34e-164 | 465 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0880046384.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047790.01 | MsG0880046384.01 | 0.800034 | 1.741938e-48 | 8.028001e-46 |
| MsG0080047927.01 | MsG0880046384.01 | 0.817163 | 3.801841e-52 | 2.747936e-49 |
| MsG0080048764.01 | MsG0880046384.01 | -0.833789 | 4.358879e-56 | 5.073025e-53 |
| MsG0180000010.01 | MsG0880046384.01 | 0.818180 | 2.242638e-52 | 1.666776e-49 |
| MsG0180000240.01 | MsG0880046384.01 | 0.856751 | 2.603649e-62 | 6.277378e-59 |
| MsG0180000544.01 | MsG0880046384.01 | 0.811699 | 6.144878e-51 | 3.829743e-48 |
| MsG0180001382.01 | MsG0880046384.01 | -0.805622 | 1.221127e-49 | 6.491135e-47 |
| MsG0880044062.01 | MsG0880046384.01 | 0.810864 | 9.326120e-51 | 5.686098e-48 |
| MsG0880044389.01 | MsG0880046384.01 | 0.811456 | 6.939854e-51 | 4.297873e-48 |
| MsG0880045605.01 | MsG0880046384.01 | 0.843423 | 1.414127e-58 | 2.211553e-55 |
| MsG0880046384.01 | MsG0880046963.01 | 0.839602 | 1.436545e-57 | 1.993738e-54 |
| MsG0880046384.01 | MsG0880047427.01 | -0.818675 | 1.731874e-52 | 1.304872e-49 |
| MsG0880046384.01 | MsG0880047689.01 | 0.869965 | 2.084722e-66 | 7.971803e-63 |
| MsG0880046384.01 | MsG0880047763.01 | -0.808869 | 2.505824e-50 | 1.449005e-47 |
| MsG0180002750.01 | MsG0880046384.01 | 0.839257 | 1.765809e-57 | 2.424907e-54 |
| MsG0180002973.01 | MsG0880046384.01 | -0.801413 | 9.112449e-49 | 4.348911e-46 |
| MsG0180003189.01 | MsG0880046384.01 | -0.819016 | 1.448798e-52 | 1.102060e-49 |
| MsG0180003246.01 | MsG0880046384.01 | 0.816827 | 4.524008e-52 | 3.239899e-49 |
| MsG0180003309.01 | MsG0880046384.01 | 0.825006 | 5.930342e-54 | 5.339796e-51 |
| MsG0180003854.01 | MsG0880046384.01 | -0.821127 | 4.762391e-53 | 3.841236e-50 |
| MsG0180005382.01 | MsG0880046384.01 | -0.804164 | 2.463422e-49 | 1.261230e-46 |
| MsG0180005515.01 | MsG0880046384.01 | 0.809075 | 2.263360e-50 | 1.316076e-47 |
| MsG0180005676.01 | MsG0880046384.01 | 0.805284 | 1.437728e-49 | 7.576035e-47 |
| MsG0180005881.01 | MsG0880046384.01 | 0.838094 | 3.526157e-57 | 4.673363e-54 |
| MsG0280006306.01 | MsG0880046384.01 | 0.800185 | 1.622939e-48 | 7.508277e-46 |
| MsG0780040476.01 | MsG0880046384.01 | 0.805348 | 1.393862e-49 | 7.357239e-47 |
| MsG0780040566.01 | MsG0880046384.01 | 0.801793 | 7.614402e-49 | 3.669113e-46 |
| MsG0780040576.01 | MsG0880046384.01 | 0.821571 | 3.760883e-53 | 3.071092e-50 |
| MsG0780041015.01 | MsG0880046384.01 | 0.800629 | 1.317499e-48 | 6.164507e-46 |
| MsG0780041441.01 | MsG0880046384.01 | -0.804028 | 2.629145e-49 | 1.341416e-46 |
| MsG0780041729.01 | MsG0880046384.01 | 0.812473 | 4.165176e-51 | 2.650888e-48 |
| MsG0280011059.01 | MsG0880046384.01 | 0.866487 | 2.749882e-65 | 9.283957e-62 |
| MsG0380014225.01 | MsG0880046384.01 | 0.823798 | 1.140715e-53 | 9.923137e-51 |
| MsG0380014401.01 | MsG0880046384.01 | 0.820206 | 7.751765e-53 | 6.094363e-50 |
| MsG0380014551.01 | MsG0880046384.01 | 0.802405 | 5.698451e-49 | 2.789151e-46 |
| MsG0380014770.01 | MsG0880046384.01 | -0.813075 | 3.074903e-51 | 1.988206e-48 |
| MsG0680031333.01 | MsG0880046384.01 | 0.830063 | 3.626738e-55 | 3.779662e-52 |
| MsG0680031446.01 | MsG0880046384.01 | 0.832917 | 7.190804e-56 | 8.152673e-53 |
| MsG0680031646.01 | MsG0880046384.01 | -0.802895 | 4.515026e-49 | 2.237623e-46 |
| MsG0680031805.01 | MsG0880046384.01 | 0.805100 | 1.570987e-49 | 8.239527e-47 |
| MsG0680032510.01 | MsG0880046384.01 | 0.801624 | 8.246425e-49 | 3.956539e-46 |
| MsG0680033391.01 | MsG0880046384.01 | -0.804708 | 1.897261e-49 | 9.850903e-47 |
| MsG0480019163.01 | MsG0880046384.01 | 0.856800 | 2.518404e-62 | 6.081796e-59 |
| MsG0480019689.01 | MsG0880046384.01 | 0.825059 | 5.762191e-54 | 5.195904e-51 |
| MsG0480021776.01 | MsG0880046384.01 | 0.851946 | 6.372017e-61 | 1.310564e-57 |
| MsG0480021845.01 | MsG0880046384.01 | 0.820194 | 7.803436e-53 | 6.133128e-50 |
| MsG0480022052.01 | MsG0880046384.01 | 0.845840 | 3.159598e-59 | 5.334877e-56 |
| MsG0480022615.01 | MsG0880046384.01 | 0.823323 | 1.473218e-53 | 1.264321e-50 |
| MsG0480022956.01 | MsG0880046384.01 | 0.828070 | 1.102599e-54 | 1.084291e-51 |
| MsG0480023814.01 | MsG0880046384.01 | -0.825420 | 4.734897e-54 | 4.313399e-51 |
| MsG0480023943.01 | MsG0880046384.01 | -0.815745 | 7.896083e-52 | 5.490467e-49 |
| MsG0580024363.01 | MsG0880046384.01 | 0.801236 | 9.902305e-49 | 4.705064e-46 |
| MsG0580024928.01 | MsG0880046384.01 | 0.847122 | 1.411900e-59 | 2.484517e-56 |
| MsG0580024930.01 | MsG0880046384.01 | 0.811660 | 6.266214e-51 | 3.901316e-48 |
| MsG0580025182.01 | MsG0880046384.01 | 0.829512 | 4.937816e-55 | 5.064525e-52 |
| MsG0580025448.01 | MsG0880046384.01 | -0.814085 | 1.843985e-51 | 1.225479e-48 |
| MsG0380015849.01 | MsG0880046384.01 | 0.845642 | 3.575195e-59 | 5.997645e-56 |
| MsG0380016191.01 | MsG0880046384.01 | 0.847487 | 1.120880e-59 | 1.995477e-56 |
| MsG0380016347.01 | MsG0880046384.01 | 0.847515 | 1.101396e-59 | 1.962605e-56 |
| MsG0380016828.01 | MsG0880046384.01 | 0.867980 | 9.170823e-66 | 3.263101e-62 |
| MsG0380017023.01 | MsG0880046384.01 | -0.803622 | 3.193212e-49 | 1.612267e-46 |
| MsG0380017263.01 | MsG0880046384.01 | 0.879709 | 9.983375e-70 | 5.468734e-66 |
| MsG0380017551.01 | MsG0880046384.01 | 0.803418 | 3.519974e-49 | 1.767946e-46 |
| MsG0580026777.01 | MsG0880046384.01 | -0.810916 | 9.085538e-51 | 5.547321e-48 |
| MsG0580028613.01 | MsG0880046384.01 | 0.868079 | 8.519377e-66 | 3.042390e-62 |
| MsG0580028901.01 | MsG0880046384.01 | -0.828277 | 9.830142e-55 | 9.725092e-52 |
| MsG0580029514.01 | MsG0880046384.01 | 0.810459 | 1.140518e-50 | 6.878538e-48 |
| MsG0580029999.01 | MsG0880046384.01 | 0.815194 | 1.047668e-51 | 7.174449e-49 |
| MsG0280006526.01 | MsG0880046384.01 | 0.811694 | 6.157672e-51 | 3.837309e-48 |
| MsG0280007743.01 | MsG0880046384.01 | 0.813716 | 2.223086e-51 | 1.462807e-48 |
| MsG0280008660.01 | MsG0880046384.01 | 0.836094 | 1.144291e-56 | 1.427283e-53 |
| MsG0280009395.01 | MsG0880046384.01 | 0.806063 | 9.868979e-50 | 5.305677e-47 |
| MsG0280009637.01 | MsG0880046384.01 | 0.811104 | 8.272688e-51 | 5.075937e-48 |
| MsG0280010146.01 | MsG0880046384.01 | 0.824803 | 6.621055e-54 | 5.927278e-51 |
| MsG0680035704.01 | MsG0880046384.01 | -0.822331 | 2.508665e-53 | 2.093395e-50 |
| MsG0780036184.01 | MsG0880046384.01 | 0.804321 | 2.285228e-49 | 1.174704e-46 |
| MsG0780037284.01 | MsG0880046384.01 | 0.812092 | 5.045863e-51 | 3.178329e-48 |
| MsG0780038309.01 | MsG0880046384.01 | 0.831529 | 1.585551e-55 | 1.725305e-52 |
| MsG0780039867.01 | MsG0880046384.01 | 0.846350 | 2.295302e-59 | 3.939493e-56 |
| MsG0780040148.01 | MsG0880046384.01 | 0.814463 | 1.521053e-51 | 1.021329e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046384.01.T01 | MTR_4g107230 | 89.078 | 293 | 9 | 5 | 1 | 276 | 1 | 287 | 7.18e-165 | 458 |
| MsG0880046384.01.T01 | MTR_5g037080 | 48.302 | 265 | 98 | 8 | 6 | 246 | 19 | 268 | 3.66e-50 | 167 |
| MsG0880046384.01.T01 | MTR_4g100630 | 57.746 | 142 | 52 | 4 | 18 | 155 | 25 | 162 | 1.61e-47 | 161 |
| MsG0880046384.01.T01 | MTR_2g100930 | 43.684 | 190 | 67 | 5 | 3 | 167 | 15 | 189 | 1.54e-42 | 149 |
| MsG0880046384.01.T01 | MTR_0036s0260 | 91.176 | 68 | 6 | 0 | 88 | 155 | 28 | 95 | 2.95e-38 | 134 |
| MsG0880046384.01.T01 | MTR_6g092540 | 51.020 | 147 | 56 | 4 | 13 | 152 | 24 | 161 | 6.64e-38 | 135 |
| MsG0880046384.01.T01 | MTR_2g090305 | 88.235 | 68 | 8 | 0 | 88 | 155 | 28 | 95 | 5.34e-36 | 127 |
| MsG0880046384.01.T01 | MTR_4g111975 | 51.163 | 129 | 49 | 3 | 81 | 209 | 131 | 245 | 1.71e-34 | 126 |
| MsG0880046384.01.T01 | MTR_3g462790 | 61.702 | 94 | 32 | 1 | 76 | 165 | 135 | 228 | 1.73e-34 | 127 |
| MsG0880046384.01.T01 | MTR_8g101650 | 73.239 | 71 | 18 | 1 | 82 | 152 | 99 | 168 | 3.64e-34 | 125 |
| MsG0880046384.01.T01 | MTR_1g111830 | 59.223 | 103 | 40 | 2 | 81 | 181 | 117 | 219 | 1.63e-33 | 122 |
| MsG0880046384.01.T01 | MTR_5g069710 | 65.854 | 82 | 28 | 0 | 77 | 158 | 121 | 202 | 1.28e-32 | 122 |
| MsG0880046384.01.T01 | MTR_7g067080 | 48.333 | 120 | 59 | 1 | 76 | 192 | 128 | 247 | 2.41e-32 | 121 |
| MsG0880046384.01.T01 | MTR_5g081860 | 61.176 | 85 | 31 | 1 | 76 | 158 | 113 | 197 | 6.43e-30 | 114 |
| MsG0880046384.01.T01 | MTR_3g104370 | 53.333 | 90 | 39 | 1 | 69 | 155 | 72 | 161 | 3.17e-27 | 105 |
| MsG0880046384.01.T01 | MTR_8g063600 | 60.811 | 74 | 27 | 1 | 82 | 153 | 100 | 173 | 5.36e-25 | 101 |
| MsG0880046384.01.T01 | MTR_5g027570 | 32.500 | 160 | 78 | 4 | 48 | 177 | 27 | 186 | 6.50e-24 | 97.1 |
| MsG0880046384.01.T01 | MTR_5g027550 | 45.631 | 103 | 49 | 3 | 70 | 166 | 60 | 161 | 9.82e-22 | 91.3 |
| MsG0880046384.01.T01 | MTR_5g088010 | 44.944 | 89 | 43 | 2 | 82 | 164 | 54 | 142 | 2.78e-20 | 87.8 |
| MsG0880046384.01.T01 | MTR_8g063870 | 45.545 | 101 | 47 | 2 | 90 | 182 | 94 | 194 | 4.50e-20 | 87.4 |
| MsG0880046384.01.T01 | MTR_1g048660 | 56.250 | 64 | 28 | 0 | 89 | 152 | 13 | 76 | 1.54e-19 | 81.6 |
| MsG0880046384.01.T01 | MTR_1g033620 | 56.338 | 71 | 31 | 0 | 104 | 174 | 21 | 91 | 5.91e-19 | 84.0 |
| MsG0880046384.01.T01 | MTR_5g088060 | 48.148 | 81 | 36 | 2 | 90 | 164 | 274 | 354 | 1.28e-18 | 85.5 |
| MsG0880046384.01.T01 | MTR_5g488160 | 48.148 | 81 | 36 | 2 | 90 | 164 | 274 | 354 | 1.28e-18 | 85.5 |
| MsG0880046384.01.T01 | MTR_4g015130 | 64.286 | 56 | 20 | 0 | 98 | 153 | 3 | 58 | 3.63e-18 | 77.4 |
| MsG0880046384.01.T01 | MTR_1g083180 | 51.429 | 70 | 30 | 2 | 90 | 156 | 63 | 131 | 4.12e-15 | 72.4 |
| MsG0880046384.01.T01 | MTR_5g075760 | 36.752 | 117 | 64 | 4 | 59 | 173 | 78 | 186 | 1.88e-14 | 70.9 |
| MsG0880046384.01.T01 | MTR_3g070110 | 48.611 | 72 | 30 | 1 | 90 | 154 | 97 | 168 | 4.47e-13 | 67.0 |
| MsG0880046384.01.T01 | MTR_5g075790 | 43.023 | 86 | 41 | 3 | 90 | 173 | 115 | 194 | 6.42e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046384.01.T01 | AT1G74840 | 58.182 | 220 | 74 | 9 | 8 | 216 | 16 | 228 | 8.95e-59 | 188 |
| MsG0880046384.01.T01 | AT1G74840 | 65.241 | 187 | 52 | 7 | 8 | 189 | 16 | 194 | 2.92e-58 | 186 |
| MsG0880046384.01.T01 | AT1G19000 | 46.154 | 299 | 121 | 9 | 1 | 276 | 4 | 285 | 5.33e-55 | 179 |
| MsG0880046384.01.T01 | AT1G19000 | 46.154 | 299 | 121 | 9 | 1 | 276 | 4 | 285 | 5.33e-55 | 179 |
| MsG0880046384.01.T01 | AT5G47390 | 54.658 | 161 | 63 | 3 | 18 | 173 | 25 | 180 | 5.08e-52 | 174 |
| MsG0880046384.01.T01 | AT1G70000 | 61.988 | 171 | 43 | 6 | 5 | 168 | 23 | 178 | 2.28e-50 | 167 |
| MsG0880046384.01.T01 | AT1G70000 | 61.988 | 171 | 43 | 6 | 5 | 168 | 23 | 178 | 2.28e-50 | 167 |
| MsG0880046384.01.T01 | AT3G16350 | 46.000 | 200 | 80 | 7 | 18 | 196 | 49 | 241 | 1.57e-38 | 139 |
| MsG0880046384.01.T01 | AT1G49010 | 72.973 | 74 | 20 | 0 | 81 | 154 | 127 | 200 | 3.23e-34 | 126 |
| MsG0880046384.01.T01 | AT2G38090 | 58.763 | 97 | 40 | 0 | 65 | 161 | 118 | 214 | 5.87e-34 | 125 |
| MsG0880046384.01.T01 | AT5G04760 | 64.368 | 87 | 30 | 1 | 76 | 161 | 87 | 173 | 1.30e-33 | 122 |
| MsG0880046384.01.T01 | AT5G58900 | 62.222 | 90 | 29 | 1 | 76 | 165 | 129 | 213 | 1.69e-33 | 124 |
| MsG0880046384.01.T01 | AT5G61620 | 39.362 | 188 | 83 | 6 | 4 | 177 | 22 | 192 | 3.79e-33 | 123 |
| MsG0880046384.01.T01 | AT5G08520 | 70.270 | 74 | 22 | 0 | 81 | 154 | 111 | 184 | 7.53e-33 | 122 |
| MsG0880046384.01.T01 | AT5G56840 | 57.282 | 103 | 32 | 3 | 63 | 164 | 73 | 164 | 4.82e-32 | 118 |
| MsG0880046384.01.T01 | AT3G11280 | 41.844 | 141 | 72 | 1 | 75 | 215 | 114 | 244 | 4.13e-30 | 114 |
| MsG0880046384.01.T01 | AT3G11280 | 41.844 | 141 | 72 | 1 | 75 | 215 | 114 | 244 | 4.13e-30 | 114 |
| MsG0880046384.01.T01 | AT5G05790 | 61.250 | 80 | 31 | 0 | 75 | 154 | 118 | 197 | 2.32e-29 | 112 |
| MsG0880046384.01.T01 | AT5G05790 | 61.250 | 80 | 31 | 0 | 75 | 154 | 118 | 197 | 2.32e-29 | 112 |
| MsG0880046384.01.T01 | AT5G01200 | 64.865 | 74 | 26 | 0 | 81 | 154 | 139 | 212 | 2.54e-29 | 112 |
| MsG0880046384.01.T01 | AT3G10580 | 60.000 | 70 | 26 | 1 | 84 | 153 | 91 | 158 | 4.68e-23 | 95.5 |
| MsG0880046384.01.T01 | AT3G10580 | 60.000 | 70 | 26 | 1 | 84 | 153 | 91 | 158 | 6.82e-23 | 95.5 |
| MsG0880046384.01.T01 | AT4G09450 | 50.000 | 94 | 46 | 1 | 69 | 161 | 71 | 164 | 8.73e-23 | 93.2 |
| MsG0880046384.01.T01 | AT5G23650 | 58.333 | 72 | 30 | 0 | 82 | 153 | 114 | 185 | 2.65e-22 | 94.7 |
| MsG0880046384.01.T01 | AT3G10590 | 46.512 | 86 | 45 | 1 | 76 | 161 | 99 | 183 | 3.29e-19 | 84.0 |
Find 66 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGTTCGCGGTCGCGGTTTC+TGG | 0.130907 | 8:-72707926 | None:intergenic |
| ATCAGACACAATGGAAGCTT+TGG | 0.326101 | 8:-72708988 | None:intergenic |
| GGAAGAGCACAAGCTGTTCT+TGG | 0.356599 | 8:+72708069 | MsG0880046384.01.T01:CDS |
| TTGACCTGTTCTTCCTCCAT+TGG | 0.357105 | 8:-72708807 | None:intergenic |
| GATTGAATAGATTGTTGGTT+TGG | 0.370712 | 8:-72708941 | None:intergenic |
| TCTGAAACAACAAAAGGAAT+TGG | 0.370883 | 8:-72708963 | None:intergenic |
| TCGAACCTCGTTTACGTTCG+CGG | 0.379501 | 8:-72707939 | None:intergenic |
| GTCATCGGCGGAGGCGTAAC+CGG | 0.383661 | 8:-72707884 | None:intergenic |
| CTCGTTTACGTTCGCGGTCG+CGG | 0.408987 | 8:-72707933 | None:intergenic |
| TTGGTTGGATTGCAGAAAGT+TGG | 0.429183 | 8:+72708088 | MsG0880046384.01.T01:CDS |
| AATTGGATTGAATAGATTGT+TGG | 0.429941 | 8:-72708946 | None:intergenic |
| ACCGCAGACTCCACCGTCTC+CGG | 0.449707 | 8:+72707715 | MsG0880046384.01.T01:CDS |
| AATTCCAATGGAGGAAGAAC+AGG | 0.463202 | 8:+72708803 | MsG0880046384.01.T01:CDS |
| TCAGACACAATGGAAGCTTT+GGG | 0.470464 | 8:-72708987 | None:intergenic |
| AGAAAGTTGGAAAAGGTGAT+TGG | 0.477943 | 8:+72708101 | MsG0880046384.01.T01:CDS |
| TGGAGGGTCAATGGATGAAC+TGG | 0.479134 | 8:-72709024 | None:intergenic |
| GGAAAGTGTTGGAGGGTCAA+TGG | 0.479524 | 8:-72709033 | None:intergenic |
| GAGCACAAGCTGTTCTTGGT+TGG | 0.483963 | 8:+72708073 | MsG0880046384.01.T01:CDS |
| CCAATGGAAGAACTCACTCT+TGG | 0.488703 | 8:+72708894 | MsG0880046384.01.T01:CDS |
| AGGAACGGCGTCATCGGCGG+AGG | 0.501551 | 8:-72707893 | None:intergenic |
| TCCGGCGAATTCATGCTCTT+CGG | 0.514098 | 8:+72707733 | MsG0880046384.01.T01:CDS |
| CCAAGAGTGAGTTCTTCCAT+TGG | 0.515535 | 8:-72708894 | None:intergenic |
| GAGCATGAATTCGCCGGAGA+CGG | 0.519327 | 8:-72707728 | None:intergenic |
| TTTCCTTCTCGTCCGATCAA+AGG | 0.521674 | 8:+72709063 | MsG0880046384.01.T01:CDS |
| GACCCTCCAACACTTTCCCT+CGG | 0.522607 | 8:+72709038 | MsG0880046384.01.T01:CDS |
| TTGGGATCTGAAACAACAAA+AGG | 0.525424 | 8:-72708969 | None:intergenic |
| TGGATCAGGGATTCCGTGGA+CGG | 0.525462 | 8:+72708045 | MsG0880046384.01.T01:intron |
| TGATGATGTCTTCCTTTGAT+CGG | 0.527840 | 8:-72709075 | None:intergenic |
| ATGATGTTGTTGTTGTCTTG+AGG | 0.532951 | 8:-72707826 | None:intergenic |
| GGTGGTGATATCAAAGAGAC+TGG | 0.540239 | 8:-72708233 | None:intergenic |
| TTGTGCTCTTCCTCCGTCCA+CGG | 0.546086 | 8:-72708058 | None:intergenic |
| GGATTGCAGAAAGTTGGAAA+AGG | 0.553347 | 8:+72708094 | MsG0880046384.01.T01:CDS |
| AATCAATGGATTTACTCCAA+TGG | 0.559709 | 8:+72708878 | MsG0880046384.01.T01:CDS |
| AGTCCGAGGGAAAGTGTTGG+AGG | 0.562456 | 8:-72709041 | None:intergenic |
| TTCGCGGTCGCGGTTTCTGG+CGG | 0.563265 | 8:-72707923 | None:intergenic |
| CATGAATTCGCCGGAGACGG+TGG | 0.565729 | 8:-72707725 | None:intergenic |
| TCAGGTATCTGCAATTCCAA+TGG | 0.575185 | 8:+72708791 | MsG0880046384.01.T01:intron |
| CTTCCTTTGATCGGACGAGA+AGG | 0.575650 | 8:-72709066 | None:intergenic |
| GCCGGAGACGGTGGAGTCTG+CGG | 0.589898 | 8:-72707716 | None:intergenic |
| TTGAGGAACGGCGTCATCGG+CGG | 0.592610 | 8:-72707896 | None:intergenic |
| TATCAAAGAGACTGGATCTA+CGG | 0.592657 | 8:-72708225 | None:intergenic |
| TCCGAAGAGCATGAATTCGC+CGG | 0.594213 | 8:-72707734 | None:intergenic |
| AGACAACAACAACATCATCA+AGG | 0.604820 | 8:+72707831 | MsG0880046384.01.T01:CDS |
| AACAAAGACGTTATCACCGC+CGG | 0.612643 | 8:+72707865 | MsG0880046384.01.T01:CDS |
| GTGATTGGAGAGGTATCTCA+AGG | 0.614661 | 8:+72708116 | MsG0880046384.01.T01:CDS |
| AATGGATGAACTGGAACTAG+AGG | 0.623042 | 8:-72709015 | None:intergenic |
| GAAGAACTCACTCTTGGACA+AGG | 0.624406 | 8:+72708900 | MsG0880046384.01.T01:CDS |
| ATCGGCGGAGGCGTAACCGG+CGG | 0.626944 | 8:-72707881 | None:intergenic |
| CAGAAAAGTTACCGTGTCGG+TGG | 0.629241 | 8:-72708251 | None:intergenic |
| GGCGATTGAGATTACTTCGG+CGG | 0.629813 | 8:-72708198 | None:intergenic |
| CGCGACCGCGAACGTAAACG+AGG | 0.635630 | 8:+72707934 | MsG0880046384.01.T01:CDS |
| CTTTGATATCACCACCGACA+CGG | 0.638033 | 8:+72708240 | MsG0880046384.01.T01:CDS |
| GTCCGAGGGAAAGTGTTGGA+GGG | 0.638496 | 8:-72709040 | None:intergenic |
| GAAAGTCCGAGGGAAAGTGT+TGG | 0.638503 | 8:-72709044 | None:intergenic |
| TGCCAGAAAAGTTACCGTGT+CGG | 0.638678 | 8:-72708254 | None:intergenic |
| CGGACGAGAAGGAAAGTCCG+AGG | 0.639721 | 8:-72709055 | None:intergenic |
| GACGGCGATTGAGATTACTT+CGG | 0.641222 | 8:-72708201 | None:intergenic |
| GGTATCTGCAATTCCAATGG+AGG | 0.648622 | 8:+72708794 | MsG0880046384.01.T01:intron |
| AGAGGTGATATCAGACACAA+TGG | 0.652354 | 8:-72708997 | None:intergenic |
| GGACGAGAAGGAAAGTCCGA+GGG | 0.654533 | 8:-72709054 | None:intergenic |
| GTTGGAAAAGGTGATTGGAG+AGG | 0.659248 | 8:+72708106 | MsG0880046384.01.T01:CDS |
| GGAGACGGTGGAGTCTGCGG+TGG | 0.660956 | 8:-72707713 | None:intergenic |
| ATCAGGGATTCCGTGGACGG+AGG | 0.665588 | 8:+72708048 | MsG0880046384.01.T01:intron |
| GAAAACAAGAACACCTACGC+AGG | 0.669459 | 8:+72708147 | MsG0880046384.01.T01:CDS |
| AGAGACTGGATCTACGGCGA+CGG | 0.684123 | 8:-72708219 | None:intergenic |
| GCATGACTTGCAACCTGCGT+AGG | 0.689624 | 8:-72708160 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTTTTTTGTG+TGG | + | Chr8:72708453-72708472 | MsG0880046384.01.T01:intron | 10.0% |
| !! | GTGATATAAGATTAATATAT+AGG | + | Chr8:72708522-72708541 | MsG0880046384.01.T01:intron | 15.0% |
| !!! | TAATTTTCTCTCTTATTTAT+TGG | + | Chr8:72708362-72708381 | MsG0880046384.01.T01:intron | 15.0% |
| !! | AAGGAAACAAAAAAATACAA+TGG | + | Chr8:72708421-72708440 | MsG0880046384.01.T01:intron | 20.0% |
| !! | AATGCTATTAAAGATTGTAA+AGG | + | Chr8:72708402-72708421 | MsG0880046384.01.T01:intron | 20.0% |
| !! | AGATTTCTTTACAACTATAA+AGG | - | Chr8:72708483-72708502 | None:intergenic | 20.0% |
| !! | ATAAGATATATATCAACTCT+TGG | - | Chr8:72708589-72708608 | None:intergenic | 20.0% |
| ! | AATTGGATTGAATAGATTGT+TGG | - | Chr8:72708949-72708968 | None:intergenic | 25.0% |
| ! | CTTATAATAGTTGATCTATC+TGG | + | Chr8:72708604-72708623 | MsG0880046384.01.T01:intron | 25.0% |
| ! | GAAATCTAACAAAACTAGAA+TGG | + | Chr8:72708496-72708515 | MsG0880046384.01.T01:intron | 25.0% |
| ! | TAATCAATAGAGTAGGATTA+TGG | + | Chr8:72708321-72708340 | MsG0880046384.01.T01:intron | 25.0% |
| ! | TAGTTACTAATCAATAGAGT+AGG | + | Chr8:72708314-72708333 | MsG0880046384.01.T01:intron | 25.0% |
| ! | TTGTTTATACTTAGTAACAG+AGG | - | Chr8:72708645-72708664 | None:intergenic | 25.0% |
| !!! | GTGATTATCGTTATTGATTT+TGG | + | Chr8:72708025-72708044 | MsG0880046384.01.T01:intron | 25.0% |
| !!! | TTATCTCCATTTTTGAAACA+TGG | - | Chr8:72709119-72709138 | None:intergenic | 25.0% |
| AACAACTTTGTGTTTATGTC+AGG | + | Chr8:72708773-72708792 | MsG0880046384.01.T01:intron | 30.0% | |
| ATGCATTTCAAAGAGTTTAC+TGG | + | Chr8:72708563-72708582 | MsG0880046384.01.T01:intron | 30.0% | |
| ! | AATCAATGGATTTACTCCAA+TGG | + | Chr8:72708878-72708897 | MsG0880046384.01.T01:CDS | 30.0% |
| ! | GATTGAATAGATTGTTGGTT+TGG | - | Chr8:72708944-72708963 | None:intergenic | 30.0% |
| ! | TCTGAAACAACAAAAGGAAT+TGG | - | Chr8:72708966-72708985 | None:intergenic | 30.0% |
| !!! | ATCGTTATTGATTTTGGATC+AGG | + | Chr8:72708031-72708050 | MsG0880046384.01.T01:intron | 30.0% |
| !!! | TCCATTGATTTTTCTAGCTT+CGG | - | Chr8:72708868-72708887 | None:intergenic | 30.0% |
| !!! | TCGTTATTGATTTTGGATCA+GGG | + | Chr8:72708032-72708051 | MsG0880046384.01.T01:intron | 30.0% |
| AGAAAGTTGGAAAAGGTGAT+TGG | + | Chr8:72708101-72708120 | MsG0880046384.01.T01:CDS | 35.0% | |
| AGACAACAACAACATCATCA+AGG | + | Chr8:72707831-72707850 | MsG0880046384.01.T01:CDS | 35.0% | |
| GCAATGCCATGTTTCAAAAA+TGG | + | Chr8:72709110-72709129 | MsG0880046384.01.T01:CDS | 35.0% | |
| TATCAAAGAGACTGGATCTA+CGG | - | Chr8:72708228-72708247 | None:intergenic | 35.0% | |
| TTCGATCAATCTCTTCAATC+TGG | + | Chr8:72707963-72707982 | MsG0880046384.01.T01:intron | 35.0% | |
| ! | ATGATGTTGTTGTTGTCTTG+AGG | - | Chr8:72707829-72707848 | None:intergenic | 35.0% |
| ! | TGATGATGTCTTCCTTTGAT+CGG | - | Chr8:72709078-72709097 | None:intergenic | 35.0% |
| ! | TTGGGATCTGAAACAACAAA+AGG | - | Chr8:72708972-72708991 | None:intergenic | 35.0% |
| !!! | ATTGATTTTTCTAGCTTCGG+GGG | - | Chr8:72708865-72708884 | None:intergenic | 35.0% |
| !!! | CATTGATTTTTCTAGCTTCG+GGG | - | Chr8:72708866-72708885 | None:intergenic | 35.0% |
| !!! | CCATTGATTTTTCTAGCTTC+GGG | - | Chr8:72708867-72708886 | None:intergenic | 35.0% |
| AATGGATGAACTGGAACTAG+AGG | - | Chr8:72709018-72709037 | None:intergenic | 40.0% | |
| AATTCCAATGGAGGAAGAAC+AGG | + | Chr8:72708803-72708822 | MsG0880046384.01.T01:CDS | 40.0% | |
| AGAGGTGATATCAGACACAA+TGG | - | Chr8:72709000-72709019 | None:intergenic | 40.0% | |
| ATCAGACACAATGGAAGCTT+TGG | - | Chr8:72708991-72709010 | None:intergenic | 40.0% | |
| CCCGAAGCTAGAAAAATCAA+TGG | + | Chr8:72708864-72708883 | MsG0880046384.01.T01:CDS | 40.0% | |
| GGATTGCAGAAAGTTGGAAA+AGG | + | Chr8:72708094-72708113 | MsG0880046384.01.T01:CDS | 40.0% | |
| TCAGACACAATGGAAGCTTT+GGG | - | Chr8:72708990-72709009 | None:intergenic | 40.0% | |
| TCAGGTATCTGCAATTCCAA+TGG | + | Chr8:72708791-72708810 | MsG0880046384.01.T01:intron | 40.0% | |
| TTGGTTGGATTGCAGAAAGT+TGG | + | Chr8:72708088-72708107 | MsG0880046384.01.T01:CDS | 40.0% | |
| AACAAAGACGTTATCACCGC+CGG | + | Chr8:72707865-72707884 | MsG0880046384.01.T01:CDS | 45.0% | |
| CCAAGAGTGAGTTCTTCCAT+TGG | - | Chr8:72708897-72708916 | None:intergenic | 45.0% | |
| CCAATGGAAGAACTCACTCT+TGG | + | Chr8:72708894-72708913 | MsG0880046384.01.T01:CDS | 45.0% | |
| CTTTGATATCACCACCGACA+CGG | + | Chr8:72708240-72708259 | MsG0880046384.01.T01:CDS | 45.0% | |
| GAAAACAAGAACACCTACGC+AGG | + | Chr8:72708147-72708166 | MsG0880046384.01.T01:CDS | 45.0% | |
| GAAGAACTCACTCTTGGACA+AGG | + | Chr8:72708900-72708919 | MsG0880046384.01.T01:CDS | 45.0% | |
| GACGGCGATTGAGATTACTT+CGG | - | Chr8:72708204-72708223 | None:intergenic | 45.0% | |
| GGTATCTGCAATTCCAATGG+AGG | + | Chr8:72708794-72708813 | MsG0880046384.01.T01:intron | 45.0% | |
| GGTGGTGATATCAAAGAGAC+TGG | - | Chr8:72708236-72708255 | None:intergenic | 45.0% | |
| GTTGGAAAAGGTGATTGGAG+AGG | + | Chr8:72708106-72708125 | MsG0880046384.01.T01:CDS | 45.0% | |
| TGCCAGAAAAGTTACCGTGT+CGG | - | Chr8:72708257-72708276 | None:intergenic | 45.0% | |
| TTGACCTGTTCTTCCTCCAT+TGG | - | Chr8:72708810-72708829 | None:intergenic | 45.0% | |
| TTTCCTTCTCGTCCGATCAA+AGG | + | Chr8:72709063-72709082 | MsG0880046384.01.T01:CDS | 45.0% | |
| ! | ATTTTGGATCAGGGATTCCG+TGG | + | Chr8:72708041-72708060 | MsG0880046384.01.T01:intron | 45.0% |
| !! | GTGATTGGAGAGGTATCTCA+AGG | + | Chr8:72708116-72708135 | MsG0880046384.01.T01:CDS | 45.0% |
| CAGAAAAGTTACCGTGTCGG+TGG | - | Chr8:72708254-72708273 | None:intergenic | 50.0% | |
| CTTCCTTTGATCGGACGAGA+AGG | - | Chr8:72709069-72709088 | None:intergenic | 50.0% | |
| GAGCACAAGCTGTTCTTGGT+TGG | + | Chr8:72708073-72708092 | MsG0880046384.01.T01:CDS | 50.0% | |
| GGCGATTGAGATTACTTCGG+CGG | - | Chr8:72708201-72708220 | None:intergenic | 50.0% | |
| TCCGGCGAATTCATGCTCTT+CGG | + | Chr8:72707733-72707752 | MsG0880046384.01.T01:CDS | 50.0% | |
| TCGAACCTCGTTTACGTTCG+CGG | - | Chr8:72707942-72707961 | None:intergenic | 50.0% | |
| TGGAGGGTCAATGGATGAAC+TGG | - | Chr8:72709027-72709046 | None:intergenic | 50.0% | |
| ! | CACCGACACGGTAACTTTTC+TGG | + | Chr8:72708252-72708271 | MsG0880046384.01.T01:intron | 50.0% |
| ! | GAAAGTCCGAGGGAAAGTGT+TGG | - | Chr8:72709047-72709066 | None:intergenic | 50.0% |
| ! | GGAAGAGCACAAGCTGTTCT+TGG | + | Chr8:72708069-72708088 | MsG0880046384.01.T01:CDS | 50.0% |
| ! | TCCGAAGAGCATGAATTCGC+CGG | - | Chr8:72707737-72707756 | None:intergenic | 50.0% |
| !! | GGAAAGTGTTGGAGGGTCAA+TGG | - | Chr8:72709036-72709055 | None:intergenic | 50.0% |
| !!! | GTTTTGAGGAACGGCGTCAT+CGG | - | Chr8:72707902-72707921 | None:intergenic | 50.0% |
| !!! | TCTGGCGGAGTTTTGAGGAA+CGG | - | Chr8:72707911-72707930 | None:intergenic | 50.0% |
| AGAGACTGGATCTACGGCGA+CGG | - | Chr8:72708222-72708241 | None:intergenic | 55.0% | |
| GACCCTCCAACACTTTCCCT+CGG | + | Chr8:72709038-72709057 | MsG0880046384.01.T01:CDS | 55.0% | |
| GAGCATGAATTCGCCGGAGA+CGG | - | Chr8:72707731-72707750 | None:intergenic | 55.0% | |
| GGACGAGAAGGAAAGTCCGA+GGG | - | Chr8:72709057-72709076 | None:intergenic | 55.0% | |
| TGGATCAGGGATTCCGTGGA+CGG | + | Chr8:72708045-72708064 | MsG0880046384.01.T01:intron | 55.0% | |
| TTGTGCTCTTCCTCCGTCCA+CGG | - | Chr8:72708061-72708080 | None:intergenic | 55.0% | |
| ! | GCATGACTTGCAACCTGCGT+AGG | - | Chr8:72708163-72708182 | None:intergenic | 55.0% |
| !! | AGTCCGAGGGAAAGTGTTGG+AGG | - | Chr8:72709044-72709063 | None:intergenic | 55.0% |
| !! | GTCCGAGGGAAAGTGTTGGA+GGG | - | Chr8:72709043-72709062 | None:intergenic | 55.0% |
| !!! | CGGTTTCTGGCGGAGTTTTG+AGG | - | Chr8:72707916-72707935 | None:intergenic | 55.0% |
| ATCAGGGATTCCGTGGACGG+AGG | + | Chr8:72708048-72708067 | MsG0880046384.01.T01:intron | 60.0% | |
| CATGAATTCGCCGGAGACGG+TGG | - | Chr8:72707728-72707747 | None:intergenic | 60.0% | |
| CGGACGAGAAGGAAAGTCCG+AGG | - | Chr8:72709058-72709077 | None:intergenic | 60.0% | |
| CTCGTTTACGTTCGCGGTCG+CGG | - | Chr8:72707936-72707955 | None:intergenic | 60.0% | |
| TTGAGGAACGGCGTCATCGG+CGG | - | Chr8:72707899-72707918 | None:intergenic | 60.0% | |
| !! | TTCTAGCTTCGGGGGCTGCA+GGG | - | Chr8:72708857-72708876 | None:intergenic | 60.0% |
| !! | TTTCTAGCTTCGGGGGCTGC+AGG | - | Chr8:72708858-72708877 | None:intergenic | 60.0% |
| ACCGCAGACTCCACCGTCTC+CGG | + | Chr8:72707715-72707734 | MsG0880046384.01.T01:CDS | 65.0% | |
| ACGTTCGCGGTCGCGGTTTC+TGG | - | Chr8:72707929-72707948 | None:intergenic | 65.0% | |
| CGCGACCGCGAACGTAAACG+AGG | + | Chr8:72707934-72707953 | MsG0880046384.01.T01:CDS | 65.0% | |
| ! | GTCATCGGCGGAGGCGTAAC+CGG | - | Chr8:72707887-72707906 | None:intergenic | 65.0% |
| ! | TTCGCGGTCGCGGTTTCTGG+CGG | - | Chr8:72707926-72707945 | None:intergenic | 65.0% |
| AGGAACGGCGTCATCGGCGG+AGG | - | Chr8:72707896-72707915 | None:intergenic | 70.0% | |
| GCCGGAGACGGTGGAGTCTG+CGG | - | Chr8:72707719-72707738 | None:intergenic | 70.0% | |
| GGAGACGGTGGAGTCTGCGG+TGG | - | Chr8:72707716-72707735 | None:intergenic | 70.0% | |
| ! | ATCGGCGGAGGCGTAACCGG+CGG | - | Chr8:72707884-72707903 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 72707697 | 72709157 | 72707697 | ID=MsG0880046384.01;Name=MsG0880046384.01 |
| Chr8 | mRNA | 72707697 | 72709157 | 72707697 | ID=MsG0880046384.01.T01;Parent=MsG0880046384.01;Name=MsG0880046384.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|276 |
| Chr8 | exon | 72707697 | 72707955 | 72707697 | ID=MsG0880046384.01.T01:exon:3945;Parent=MsG0880046384.01.T01 |
| Chr8 | exon | 72708053 | 72708261 | 72708053 | ID=MsG0880046384.01.T01:exon:3946;Parent=MsG0880046384.01.T01 |
| Chr8 | exon | 72708795 | 72709157 | 72708795 | ID=MsG0880046384.01.T01:exon:3947;Parent=MsG0880046384.01.T01 |
| Chr8 | CDS | 72707697 | 72707955 | 72707697 | ID=MsG0880046384.01.T01:cds;Parent=MsG0880046384.01.T01 |
| Chr8 | CDS | 72708053 | 72708261 | 72708053 | ID=MsG0880046384.01.T01:cds;Parent=MsG0880046384.01.T01 |
| Chr8 | CDS | 72708795 | 72709157 | 72708795 | ID=MsG0880046384.01.T01:cds;Parent=MsG0880046384.01.T01 |
| Gene Sequence |
| Protein sequence |
