Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022956.01.T01 | XP_024629411.1 | 94.805 | 77 | 4 | 0 | 1 | 77 | 53 | 129 | 8.76E-46 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022956.01.T01 | A0A072TUR3 | 94.805 | 77 | 4 | 0 | 1 | 77 | 53 | 129 | 4.26e-46 | 158 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049069.01 | MsG0480022956.01 | -0.803094 | 4.107226e-49 | 2.045693e-46 |
| MsG0180000010.01 | MsG0480022956.01 | 0.819185 | 1.326375e-52 | 1.013499e-49 |
| MsG0180000544.01 | MsG0480022956.01 | 0.860018 | 2.767399e-63 | 7.456725e-60 |
| MsG0180001248.01 | MsG0480022956.01 | 0.800829 | 1.199191e-48 | 5.639441e-46 |
| MsG0180003246.01 | MsG0480022956.01 | 0.814775 | 1.297170e-51 | 8.783195e-49 |
| MsG0180003767.01 | MsG0480022956.01 | 0.808362 | 3.214408e-50 | 1.834379e-47 |
| MsG0180005515.01 | MsG0480022956.01 | 0.834334 | 3.183723e-56 | 3.766580e-53 |
| MsG0180005881.01 | MsG0480022956.01 | 0.822575 | 2.201034e-53 | 1.849679e-50 |
| MsG0180006034.01 | MsG0480022956.01 | 0.855615 | 5.603395e-62 | 1.301223e-58 |
| MsG0280006306.01 | MsG0480022956.01 | 0.822896 | 1.853002e-53 | 1.571290e-50 |
| MsG0280010604.01 | MsG0480022956.01 | 0.814923 | 1.203177e-51 | 8.178923e-49 |
| MsG0280011059.01 | MsG0480022956.01 | 0.847525 | 1.094395e-59 | 1.950845e-56 |
| MsG0380014401.01 | MsG0480022956.01 | 0.832791 | 7.728038e-56 | 8.728886e-53 |
| MsG0480019126.01 | MsG0480022956.01 | -0.812973 | 3.237158e-51 | 2.087454e-48 |
| MsG0480019163.01 | MsG0480022956.01 | 0.822993 | 1.759035e-53 | 1.495733e-50 |
| MsG0480021776.01 | MsG0480022956.01 | 0.877692 | 5.126424e-69 | 2.602285e-65 |
| MsG0480021845.01 | MsG0480022956.01 | 0.848437 | 6.133206e-60 | 1.125547e-56 |
| MsG0480022872.01 | MsG0480022956.01 | 0.854921 | 8.920500e-62 | 2.024247e-58 |
| MsG0480022939.01 | MsG0480022956.01 | 0.807293 | 5.425350e-50 | 3.010478e-47 |
| MsG0480022956.01 | MsG0480023973.01 | 0.824434 | 8.088081e-54 | 7.162891e-51 |
| MsG0480022956.01 | MsG0580024837.01 | 0.800618 | 1.324530e-48 | 6.195584e-46 |
| MsG0480022956.01 | MsG0580025182.01 | 0.845631 | 3.600592e-59 | 6.037524e-56 |
| MsG0480022956.01 | MsG0780038309.01 | 0.819981 | 8.729976e-53 | 6.820860e-50 |
| MsG0480022956.01 | MsG0780038473.01 | 0.822607 | 2.164237e-53 | 1.820405e-50 |
| MsG0480022956.01 | MsG0780040295.01 | 0.832429 | 9.500188e-56 | 1.061550e-52 |
| MsG0480022956.01 | MsG0780040476.01 | 0.822337 | 2.499959e-53 | 2.086553e-50 |
| MsG0480022956.01 | MsG0780040566.01 | 0.828264 | 9.901092e-55 | 9.791009e-52 |
| MsG0480022956.01 | MsG0780041117.01 | 0.801916 | 7.182709e-49 | 3.471983e-46 |
| MsG0480022956.01 | MsG0780041470.01 | 0.865208 | 6.976554e-65 | 2.251265e-61 |
| MsG0480022956.01 | MsG0880042987.01 | 0.845761 | 3.320293e-59 | 5.591900e-56 |
| MsG0480022956.01 | MsG0880045605.01 | 0.831996 | 1.215965e-55 | 1.341603e-52 |
| MsG0480022956.01 | MsG0880046384.01 | 0.828070 | 1.102599e-54 | 1.084291e-51 |
| MsG0480022956.01 | MsG0880046825.01 | 0.881543 | 2.199548e-70 | 1.294005e-66 |
| MsG0480022956.01 | MsG0880046963.01 | 0.837370 | 5.410230e-57 | 7.012477e-54 |
| MsG0480022956.01 | MsG0880047763.01 | -0.833401 | 5.449056e-56 | 6.268284e-53 |
| MsG0380015212.01 | MsG0480022956.01 | 0.821888 | 3.178154e-53 | 2.618686e-50 |
| MsG0380016191.01 | MsG0480022956.01 | 0.824252 | 8.928623e-54 | 7.866110e-51 |
| MsG0380016347.01 | MsG0480022956.01 | 0.816860 | 4.448108e-52 | 3.188454e-49 |
| MsG0380016828.01 | MsG0480022956.01 | 0.865555 | 5.422705e-65 | 1.771787e-61 |
| MsG0380017263.01 | MsG0480022956.01 | 0.811381 | 7.202567e-51 | 4.451686e-48 |
| MsG0380017776.01 | MsG0480022956.01 | 0.829103 | 6.207186e-55 | 6.290458e-52 |
| MsG0380017878.01 | MsG0480022956.01 | 0.813488 | 2.495248e-51 | 1.631802e-48 |
| MsG0380017891.01 | MsG0480022956.01 | 0.813706 | 2.234470e-51 | 1.469888e-48 |
| MsG0280007193.01 | MsG0480022956.01 | 0.832560 | 8.815884e-56 | 9.889910e-53 |
| MsG0280008660.01 | MsG0480022956.01 | 0.805119 | 1.556752e-49 | 8.168983e-47 |
| MsG0280008928.01 | MsG0480022956.01 | -0.813160 | 2.946585e-51 | 1.909561e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022956.01.T01 | MTR_8g080490 | 94.805 | 77 | 4 | 0 | 1 | 77 | 53 | 129 | 1.08e-49 | 158 |
| MsG0480022956.01.T01 | MTR_8g080490 | 94.805 | 77 | 4 | 0 | 1 | 77 | 53 | 129 | 1.73e-48 | 158 |
| MsG0480022956.01.T01 | MTR_3g085480 | 66.667 | 78 | 25 | 1 | 1 | 77 | 54 | 131 | 4.44e-28 | 104 |
| MsG0480022956.01.T01 | MTR_3g037720 | 43.023 | 86 | 38 | 4 | 1 | 77 | 119 | 202 | 7.77e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022956.01.T01 | AT1G67410 | 53.846 | 78 | 35 | 1 | 1 | 77 | 57 | 134 | 2.08e-25 | 95.9 |
| MsG0480022956.01.T01 | AT1G67410 | 53.846 | 78 | 35 | 1 | 1 | 77 | 57 | 134 | 8.97e-25 | 95.5 |
Find 24 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCACGGTGGCCGGGGAAT+TGG | 0.209610 | 4:+79082659 | MsG0480022956.01.T01:CDS |
| TCCACGGTGGCCGGGGAATT+GGG | 0.210132 | 4:+79082660 | MsG0480022956.01.T01:CDS |
| TGGTGAAGCTGTGAGGGTTT+TGG | 0.347300 | 4:+79082747 | MsG0480022956.01.T01:CDS |
| CGGGAGTCTCCGACGTGTTC+CGG | 0.368384 | 4:-79082621 | None:intergenic |
| GATCATTCCGACATTGAAAC+GGG | 0.374691 | 4:-79082593 | None:intergenic |
| CCACGGTGGCCGGGGAATTG+GGG | 0.447331 | 4:+79082661 | MsG0480022956.01.T01:CDS |
| TGATCATTCCGACATTGAAA+CGG | 0.458697 | 4:-79082594 | None:intergenic |
| AGCAGCATAGTGTGGAGTAT+TGG | 0.478161 | 4:+79082692 | MsG0480022956.01.T01:CDS |
| CTCCCGCCCCGTTTCAATGT+CGG | 0.519373 | 4:+79082586 | MsG0480022956.01.T01:CDS |
| GGAAAATCACTAACGGTGAC+GGG | 0.532592 | 4:-79082640 | None:intergenic |
| TGGAAAATCACTAACGGTGA+CGG | 0.533130 | 4:-79082641 | None:intergenic |
| TAGTGTGGAGTATTGGATGA+TGG | 0.537780 | 4:+79082699 | MsG0480022956.01.T01:CDS |
| TTCCGACATTGAAACGGGGC+GGG | 0.550144 | 4:-79082588 | None:intergenic |
| TTCTTCAAACCCCAATTCCC+CGG | 0.550864 | 4:-79082670 | None:intergenic |
| CCCCAATTCCCCGGCCACCG+TGG | 0.568857 | 4:-79082661 | None:intergenic |
| TGATGGTGGTGAAGCTGTGA+GGG | 0.585351 | 4:+79082741 | MsG0480022956.01.T01:CDS |
| AGTGTGGAGTATTGGATGAT+GGG | 0.598960 | 4:+79082700 | MsG0480022956.01.T01:CDS |
| TTTGAAGAAGCAGCATAGTG+TGG | 0.628924 | 4:+79082684 | MsG0480022956.01.T01:CDS |
| GTGATGGTGGTGAAGCTGTG+AGG | 0.639132 | 4:+79082740 | MsG0480022956.01.T01:CDS |
| ATGTCGGAATGATCACTCGC+CGG | 0.648453 | 4:+79082602 | MsG0480022956.01.T01:CDS |
| CCACCGTGGAAAATCACTAA+CGG | 0.670625 | 4:-79082647 | None:intergenic |
| ATTCCGACATTGAAACGGGG+CGG | 0.686198 | 4:-79082589 | None:intergenic |
| ATCATTCCGACATTGAAACG+GGG | 0.730610 | 4:-79082592 | None:intergenic |
| GATCACTCGCCGGAACACGT+CGG | 0.757783 | 4:+79082612 | MsG0480022956.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAACTCAACGAAGAAAAA+CGG | - | Chr4:79082802-79082821 | None:intergenic | 25.0% |
| GAAGAAAAACGGAACAAAAA+AGG | - | Chr4:79082791-79082810 | None:intergenic | 30.0% | |
| TGATCATTCCGACATTGAAA+CGG | - | Chr4:79082597-79082616 | None:intergenic | 35.0% | |
| ATCATTCCGACATTGAAACG+GGG | - | Chr4:79082595-79082614 | None:intergenic | 40.0% | |
| GATCATTCCGACATTGAAAC+GGG | - | Chr4:79082596-79082615 | None:intergenic | 40.0% | |
| TGGAAAATCACTAACGGTGA+CGG | - | Chr4:79082644-79082663 | None:intergenic | 40.0% | |
| TTTGAAGAAGCAGCATAGTG+TGG | + | Chr4:79082684-79082703 | MsG0480022956.01.T01:CDS | 40.0% | |
| ! | TCACCGTTAGTGATTTTCCA+CGG | + | Chr4:79082644-79082663 | MsG0480022956.01.T01:CDS | 40.0% |
| !! | AAAAAGGCATCAGCCAATTC+CGG | - | Chr4:79082775-79082794 | None:intergenic | 40.0% |
| !! | AGTGTGGAGTATTGGATGAT+GGG | + | Chr4:79082700-79082719 | MsG0480022956.01.T01:CDS | 40.0% |
| !! | TAGTGTGGAGTATTGGATGA+TGG | + | Chr4:79082699-79082718 | MsG0480022956.01.T01:CDS | 40.0% |
| AGCAGCATAGTGTGGAGTAT+TGG | + | Chr4:79082692-79082711 | MsG0480022956.01.T01:CDS | 45.0% | |
| CCACCGTGGAAAATCACTAA+CGG | - | Chr4:79082650-79082669 | None:intergenic | 45.0% | |
| GGAAAATCACTAACGGTGAC+GGG | - | Chr4:79082643-79082662 | None:intergenic | 45.0% | |
| TTCTTCAAACCCCAATTCCC+CGG | - | Chr4:79082673-79082692 | None:intergenic | 45.0% | |
| !! | ATGGGATCGCTTTTACATGC+TGG | + | Chr4:79082718-79082737 | MsG0480022956.01.T01:CDS | 45.0% |
| !! | TCGCTTTTACATGCTGGTGA+TGG | + | Chr4:79082724-79082743 | MsG0480022956.01.T01:CDS | 45.0% |
| !!! | CTTTTACATGCTGGTGATGG+TGG | + | Chr4:79082727-79082746 | MsG0480022956.01.T01:CDS | 45.0% |
| ATGTCGGAATGATCACTCGC+CGG | + | Chr4:79082602-79082621 | MsG0480022956.01.T01:CDS | 50.0% | |
| ATTCCGACATTGAAACGGGG+CGG | - | Chr4:79082592-79082611 | None:intergenic | 50.0% | |
| ! | CCGTTAGTGATTTTCCACGG+TGG | + | Chr4:79082647-79082666 | MsG0480022956.01.T01:CDS | 50.0% |
| ! | TAGTGATTTTCCACGGTGGC+CGG | + | Chr4:79082651-79082670 | MsG0480022956.01.T01:CDS | 50.0% |
| ! | TGGTGAAGCTGTGAGGGTTT+TGG | + | Chr4:79082747-79082766 | MsG0480022956.01.T01:CDS | 50.0% |
| !! | TGATGGTGGTGAAGCTGTGA+GGG | + | Chr4:79082741-79082760 | MsG0480022956.01.T01:CDS | 50.0% |
| !!! | AGCTGTGAGGGTTTTGGATC+CGG | + | Chr4:79082753-79082772 | MsG0480022956.01.T01:CDS | 50.0% |
| !!! | GAGGGTTTTGGATCCGGAAT+TGG | + | Chr4:79082759-79082778 | MsG0480022956.01.T01:CDS | 50.0% |
| TTCCGACATTGAAACGGGGC+GGG | - | Chr4:79082591-79082610 | None:intergenic | 55.0% | |
| ! | AGTGATTTTCCACGGTGGCC+GGG | + | Chr4:79082652-79082671 | MsG0480022956.01.T01:CDS | 55.0% |
| !! | GTGATGGTGGTGAAGCTGTG+AGG | + | Chr4:79082740-79082759 | MsG0480022956.01.T01:CDS | 55.0% |
| CTCCCGCCCCGTTTCAATGT+CGG | + | Chr4:79082586-79082605 | MsG0480022956.01.T01:CDS | 60.0% | |
| GATCACTCGCCGGAACACGT+CGG | + | Chr4:79082612-79082631 | MsG0480022956.01.T01:CDS | 60.0% | |
| ! | GTGATTTTCCACGGTGGCCG+GGG | + | Chr4:79082653-79082672 | MsG0480022956.01.T01:CDS | 60.0% |
| CGGGAGTCTCCGACGTGTTC+CGG | - | Chr4:79082624-79082643 | None:intergenic | 65.0% | |
| TCCACGGTGGCCGGGGAATT+GGG | + | Chr4:79082660-79082679 | MsG0480022956.01.T01:CDS | 65.0% | |
| TTCCACGGTGGCCGGGGAAT+TGG | + | Chr4:79082659-79082678 | MsG0480022956.01.T01:CDS | 65.0% | |
| CCACGGTGGCCGGGGAATTG+GGG | + | Chr4:79082661-79082680 | MsG0480022956.01.T01:CDS | 70.0% | |
| CCCCAATTCCCCGGCCACCG+TGG | - | Chr4:79082664-79082683 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 79082577 | 79082822 | 79082577 | ID=MsG0480022956.01;Name=MsG0480022956.01 |
| Chr4 | mRNA | 79082577 | 79082822 | 79082577 | ID=MsG0480022956.01.T01;Parent=MsG0480022956.01;Name=MsG0480022956.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr4 | exon | 79082577 | 79082822 | 79082577 | ID=MsG0480022956.01.T01:exon:6003;Parent=MsG0480022956.01.T01 |
| Chr4 | CDS | 79082577 | 79082822 | 79082577 | ID=MsG0480022956.01.T01:cds;Parent=MsG0480022956.01.T01 |
| Gene Sequence |
| Protein sequence |
