Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040594.01.T01 | XP_003625279.1 | 95.853 | 217 | 9 | 0 | 27 | 243 | 79 | 295 | 8.90E-151 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040594.01.T01 | P46665 | 67.188 | 192 | 48 | 3 | 58 | 238 | 134 | 321 | 3.03E-84 | 257 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040594.01.T01 | G7KVF5 | 95.853 | 217 | 9 | 0 | 27 | 243 | 79 | 295 | 4.25e-151 | 432 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0780040594.01.T01 | TF | HB-HD-ZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000096.01 | MsG0780040594.01 | 0.818717 | 1.694543e-52 | 1.278210e-49 |
| MsG0180004120.01 | MsG0780040594.01 | 0.809285 | 2.040386e-50 | 1.193121e-47 |
| MsG0780040460.01 | MsG0780040594.01 | 0.828841 | 7.183931e-55 | 7.223966e-52 |
| MsG0780040594.01 | MsG0880045015.01 | 0.811533 | 6.674508e-51 | 4.142078e-48 |
| MsG0680031356.01 | MsG0780040594.01 | 0.824155 | 9.408650e-54 | 8.266881e-51 |
| MsG0480023575.01 | MsG0780040594.01 | 0.816623 | 5.025974e-52 | 3.579408e-49 |
| MsG0580026029.01 | MsG0780040594.01 | 0.810409 | 1.169165e-50 | 7.041996e-48 |
| MsG0380015402.01 | MsG0780040594.01 | 0.831544 | 1.572109e-55 | 1.711510e-52 |
| MsG0380016031.01 | MsG0780040594.01 | 0.819778 | 9.712238e-53 | 7.545468e-50 |
| MsG0380016760.01 | MsG0780040594.01 | 0.811810 | 5.811632e-51 | 3.632928e-48 |
| MsG0280006717.01 | MsG0780040594.01 | 0.801937 | 7.111354e-49 | 3.439281e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040594.01.T01 | MTR_7g093430 | 95.853 | 217 | 9 | 0 | 27 | 243 | 79 | 295 | 1.08e-154 | 432 |
| MsG0780040594.01.T01 | MTR_1g054285 | 72.816 | 206 | 52 | 3 | 19 | 223 | 67 | 269 | 3.67e-96 | 283 |
| MsG0780040594.01.T01 | MTR_2g061030 | 80.263 | 152 | 20 | 2 | 85 | 233 | 181 | 325 | 1.02e-78 | 240 |
| MsG0780040594.01.T01 | MTR_4g100550 | 64.062 | 192 | 51 | 3 | 36 | 222 | 57 | 235 | 2.53e-75 | 230 |
| MsG0780040594.01.T01 | MTR_5g013010 | 62.051 | 195 | 66 | 4 | 36 | 229 | 71 | 258 | 1.95e-71 | 220 |
| MsG0780040594.01.T01 | MTR_4g097600 | 77.600 | 125 | 26 | 1 | 98 | 220 | 115 | 239 | 5.35e-67 | 208 |
| MsG0780040594.01.T01 | MTR_8g006705 | 65.341 | 176 | 48 | 3 | 54 | 219 | 86 | 258 | 7.54e-67 | 209 |
| MsG0780040594.01.T01 | MTR_5g014890 | 67.626 | 139 | 45 | 0 | 99 | 237 | 114 | 252 | 6.73e-66 | 205 |
| MsG0780040594.01.T01 | MTR_1g017090 | 62.590 | 139 | 50 | 1 | 107 | 243 | 129 | 267 | 8.70e-57 | 182 |
| MsG0780040594.01.T01 | MTR_4g084750 | 65.323 | 124 | 36 | 2 | 103 | 219 | 59 | 182 | 8.76e-50 | 162 |
| MsG0780040594.01.T01 | MTR_3g103590 | 67.939 | 131 | 40 | 2 | 99 | 228 | 136 | 265 | 1.04e-48 | 162 |
| MsG0780040594.01.T01 | MTR_2g038625 | 56.618 | 136 | 55 | 2 | 86 | 220 | 63 | 195 | 8.86e-41 | 139 |
| MsG0780040594.01.T01 | MTR_2g038580 | 56.618 | 136 | 55 | 2 | 86 | 220 | 63 | 195 | 8.86e-41 | 139 |
| MsG0780040594.01.T01 | MTR_4g126900 | 58.772 | 114 | 43 | 1 | 110 | 219 | 90 | 203 | 2.07e-36 | 128 |
| MsG0780040594.01.T01 | MTR_5g013010 | 54.839 | 124 | 49 | 3 | 36 | 159 | 71 | 187 | 2.07e-30 | 112 |
| MsG0780040594.01.T01 | MTR_6g007647 | 40.146 | 137 | 80 | 1 | 105 | 241 | 30 | 164 | 1.35e-20 | 87.8 |
| MsG0780040594.01.T01 | MTR_6g007647 | 40.146 | 137 | 80 | 1 | 105 | 241 | 64 | 198 | 2.04e-20 | 87.8 |
| MsG0780040594.01.T01 | MTR_1g061660 | 44.330 | 97 | 54 | 0 | 104 | 200 | 55 | 151 | 3.29e-20 | 86.3 |
| MsG0780040594.01.T01 | MTR_7g010020 | 44.737 | 114 | 59 | 1 | 89 | 198 | 61 | 174 | 9.49e-20 | 85.9 |
| MsG0780040594.01.T01 | MTR_5g039000 | 45.283 | 106 | 54 | 1 | 100 | 201 | 69 | 174 | 1.13e-18 | 83.2 |
| MsG0780040594.01.T01 | MTR_8g468210 | 42.623 | 122 | 63 | 3 | 87 | 201 | 53 | 174 | 2.02e-18 | 82.4 |
| MsG0780040594.01.T01 | MTR_4g107650 | 48.077 | 104 | 52 | 1 | 94 | 195 | 79 | 182 | 2.19e-18 | 83.2 |
| MsG0780040594.01.T01 | MTR_6g011610 | 45.946 | 111 | 51 | 2 | 93 | 194 | 79 | 189 | 1.29e-17 | 80.1 |
| MsG0780040594.01.T01 | MTR_5g038280 | 47.872 | 94 | 42 | 1 | 108 | 194 | 87 | 180 | 1.39e-17 | 80.9 |
| MsG0780040594.01.T01 | MTR_7g103340 | 41.935 | 93 | 54 | 0 | 103 | 195 | 58 | 150 | 2.06e-17 | 79.0 |
| MsG0780040594.01.T01 | MTR_6g011610 | 45.946 | 111 | 51 | 2 | 93 | 194 | 60 | 170 | 2.36e-17 | 79.0 |
| MsG0780040594.01.T01 | MTR_3g080100 | 45.283 | 106 | 49 | 2 | 99 | 195 | 9 | 114 | 5.02e-17 | 77.4 |
| MsG0780040594.01.T01 | MTR_3g080100 | 45.283 | 106 | 49 | 2 | 99 | 195 | 48 | 153 | 6.73e-17 | 77.8 |
| MsG0780040594.01.T01 | MTR_8g469430 | 45.745 | 94 | 44 | 1 | 108 | 194 | 89 | 182 | 2.34e-16 | 76.6 |
| MsG0780040594.01.T01 | MTR_8g089895 | 43.750 | 112 | 53 | 2 | 91 | 195 | 40 | 148 | 3.96e-16 | 76.6 |
| MsG0780040594.01.T01 | MTR_3g086790 | 35.294 | 170 | 89 | 4 | 69 | 229 | 33 | 190 | 2.72e-15 | 73.9 |
| MsG0780040594.01.T01 | MTR_8g026960 | 44.304 | 79 | 44 | 0 | 115 | 193 | 41 | 119 | 2.39e-12 | 65.1 |
| MsG0780040594.01.T01 | MTR_3g092150 | 43.333 | 90 | 44 | 1 | 111 | 193 | 41 | 130 | 3.11e-12 | 64.7 |
| MsG0780040594.01.T01 | MTR_2g101720 | 35.246 | 122 | 60 | 1 | 111 | 232 | 145 | 247 | 9.42e-12 | 64.7 |
| MsG0780040594.01.T01 | MTR_5g029320 | 35.772 | 123 | 74 | 2 | 87 | 204 | 66 | 188 | 1.32e-11 | 64.3 |
| MsG0780040594.01.T01 | MTR_5g019650 | 38.889 | 108 | 61 | 1 | 85 | 192 | 35 | 137 | 5.26e-11 | 60.8 |
| MsG0780040594.01.T01 | MTR_5g019680 | 38.182 | 110 | 63 | 1 | 85 | 194 | 35 | 139 | 9.59e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040594.01.T01 | AT5G06710 | 67.188 | 192 | 48 | 3 | 58 | 238 | 134 | 321 | 3.09e-85 | 257 |
| MsG0780040594.01.T01 | AT4G37790 | 81.967 | 122 | 22 | 0 | 99 | 220 | 117 | 238 | 1.92e-70 | 217 |
| MsG0780040594.01.T01 | AT3G60390 | 53.617 | 235 | 77 | 6 | 10 | 219 | 47 | 274 | 1.44e-69 | 216 |
| MsG0780040594.01.T01 | AT2G44910 | 51.709 | 234 | 92 | 6 | 10 | 223 | 47 | 279 | 1.27e-67 | 211 |
| MsG0780040594.01.T01 | AT5G47370 | 60.204 | 196 | 65 | 6 | 36 | 221 | 52 | 244 | 4.68e-67 | 209 |
| MsG0780040594.01.T01 | AT4G16780 | 58.462 | 195 | 67 | 4 | 56 | 242 | 70 | 258 | 8.76e-67 | 208 |
| MsG0780040594.01.T01 | AT2G22800 | 77.049 | 122 | 28 | 0 | 99 | 220 | 104 | 225 | 1.36e-66 | 207 |
| MsG0780040594.01.T01 | AT4G17460 | 59.596 | 198 | 63 | 6 | 36 | 221 | 57 | 249 | 5.63e-63 | 198 |
| MsG0780040594.01.T01 | AT2G01430 | 57.534 | 146 | 60 | 2 | 85 | 230 | 116 | 259 | 1.83e-49 | 164 |
| MsG0780040594.01.T01 | AT1G70920 | 58.015 | 131 | 46 | 2 | 92 | 220 | 21 | 144 | 1.01e-40 | 138 |
| MsG0780040594.01.T01 | AT1G70920 | 58.015 | 131 | 46 | 2 | 92 | 220 | 51 | 174 | 1.06e-40 | 139 |
| MsG0780040594.01.T01 | AT5G06710 | 62.500 | 112 | 28 | 2 | 58 | 159 | 134 | 241 | 4.28e-38 | 134 |
| MsG0780040594.01.T01 | AT2G01430 | 62.667 | 75 | 28 | 0 | 85 | 159 | 116 | 190 | 6.61e-25 | 98.6 |
| MsG0780040594.01.T01 | AT1G26960 | 39.744 | 156 | 81 | 5 | 93 | 241 | 57 | 206 | 4.55e-19 | 84.0 |
| MsG0780040594.01.T01 | AT1G69780 | 45.872 | 109 | 56 | 2 | 90 | 195 | 64 | 172 | 1.20e-18 | 83.2 |
| MsG0780040594.01.T01 | AT2G36610 | 42.157 | 102 | 51 | 1 | 103 | 196 | 66 | 167 | 6.59e-18 | 79.3 |
| MsG0780040594.01.T01 | AT3G01220 | 42.857 | 119 | 56 | 2 | 94 | 200 | 65 | 183 | 1.05e-17 | 80.5 |
| MsG0780040594.01.T01 | AT5G03790 | 43.617 | 94 | 53 | 0 | 103 | 196 | 72 | 165 | 2.54e-17 | 78.6 |
| MsG0780040594.01.T01 | AT5G06710 | 51.765 | 85 | 27 | 2 | 58 | 132 | 134 | 214 | 2.65e-17 | 78.6 |
| MsG0780040594.01.T01 | AT5G06710 | 51.765 | 85 | 27 | 2 | 58 | 132 | 134 | 214 | 2.65e-17 | 78.6 |
| MsG0780040594.01.T01 | AT5G15150 | 45.000 | 120 | 55 | 4 | 86 | 197 | 92 | 208 | 3.09e-17 | 79.7 |
| MsG0780040594.01.T01 | AT3G01470 | 46.739 | 92 | 49 | 0 | 108 | 199 | 68 | 159 | 4.30e-16 | 75.9 |
| MsG0780040594.01.T01 | AT5G65310 | 42.857 | 98 | 49 | 1 | 105 | 195 | 69 | 166 | 1.91e-15 | 74.7 |
| MsG0780040594.01.T01 | AT2G22430 | 45.455 | 99 | 50 | 1 | 104 | 198 | 58 | 156 | 2.15e-15 | 74.3 |
| MsG0780040594.01.T01 | AT5G65310 | 42.857 | 98 | 49 | 1 | 105 | 195 | 51 | 148 | 2.30e-15 | 74.3 |
| MsG0780040594.01.T01 | AT1G27045 | 56.061 | 66 | 29 | 0 | 108 | 173 | 25 | 90 | 2.41e-15 | 72.4 |
| MsG0780040594.01.T01 | AT1G27045 | 56.061 | 66 | 29 | 0 | 108 | 173 | 25 | 90 | 2.41e-15 | 72.4 |
| MsG0780040594.01.T01 | AT1G27045 | 56.061 | 66 | 29 | 0 | 108 | 173 | 17 | 82 | 3.01e-15 | 71.6 |
| MsG0780040594.01.T01 | AT1G27045 | 56.061 | 66 | 29 | 0 | 108 | 173 | 68 | 133 | 3.66e-15 | 72.8 |
| MsG0780040594.01.T01 | AT1G27045 | 56.061 | 66 | 29 | 0 | 108 | 173 | 68 | 133 | 5.44e-15 | 72.4 |
| MsG0780040594.01.T01 | AT4G40060 | 42.857 | 98 | 52 | 1 | 104 | 197 | 55 | 152 | 2.11e-14 | 71.6 |
| MsG0780040594.01.T01 | AT2G18550 | 45.361 | 97 | 52 | 1 | 98 | 194 | 52 | 147 | 3.73e-14 | 69.7 |
| MsG0780040594.01.T01 | AT2G46680 | 41.758 | 91 | 53 | 0 | 103 | 193 | 27 | 117 | 5.38e-14 | 69.7 |
| MsG0780040594.01.T01 | AT2G46680 | 41.758 | 91 | 53 | 0 | 103 | 193 | 27 | 117 | 6.27e-14 | 69.7 |
| MsG0780040594.01.T01 | AT5G03790 | 42.045 | 88 | 51 | 0 | 103 | 190 | 72 | 159 | 6.85e-14 | 68.6 |
| MsG0780040594.01.T01 | AT4G36740 | 41.837 | 98 | 56 | 1 | 97 | 194 | 46 | 142 | 1.98e-12 | 65.1 |
| MsG0780040594.01.T01 | AT4G36740 | 41.837 | 98 | 56 | 1 | 97 | 194 | 45 | 141 | 2.13e-12 | 64.7 |
| MsG0780040594.01.T01 | AT3G61890 | 42.169 | 83 | 48 | 0 | 111 | 193 | 33 | 115 | 6.52e-12 | 63.9 |
Find 69 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATCTAAAATAAGAAAAT+TGG | 0.146930 | 7:-79681007 | None:intergenic |
| CTCTTCTCGACTACTGTTTC+TGG | 0.172775 | 7:-79679580 | None:intergenic |
| TACTTATATGTGTATGTTTA+CGG | 0.232367 | 7:+79679392 | MsG0780040594.01.T01:CDS |
| ATAAGAACCGTTGTCATTAT+TGG | 0.263146 | 7:-79680893 | None:intergenic |
| ACCAGCGATGATGAAGAAAA+TGG | 0.272695 | 7:+79679618 | MsG0780040594.01.T01:CDS |
| TATAGCCAAGGGCAATAATC+TGG | 0.313688 | 7:-79680947 | None:intergenic |
| AATATGACGCTGCACTATTC+GGG | 0.319466 | 7:-79679512 | None:intergenic |
| CTCTAACAGAAGAAAATAAA+AGG | 0.327202 | 7:+79680700 | MsG0780040594.01.T01:CDS |
| CCACGGACGCCAACCGGAAT+TGG | 0.334110 | 7:-79679455 | None:intergenic |
| TAATATGACGCTGCACTATT+CGG | 0.334434 | 7:-79679513 | None:intergenic |
| GAAGAAACTCAGACTCTCTA+AGG | 0.373372 | 7:+79679650 | MsG0780040594.01.T01:CDS |
| TTGTTTAGTGGCAGTGGTTC+CGG | 0.381277 | 7:-79680841 | None:intergenic |
| TATCGGATGTGAACCAATTC+CGG | 0.385580 | 7:+79679442 | MsG0780040594.01.T01:CDS |
| CACTTCCGTTTGCTTTAACT+TGG | 0.385691 | 7:-79680646 | None:intergenic |
| GTCCACGTCAAGTAGAAGTT+TGG | 0.395922 | 7:+79680446 | MsG0780040594.01.T01:CDS |
| AATCGAGATGGTTTGTTTAG+TGG | 0.400673 | 7:-79680853 | None:intergenic |
| TCATTATTGGCGGTGCCCAC+CGG | 0.402169 | 7:-79680880 | None:intergenic |
| TATCCTCCACGGACGCCAAC+CGG | 0.430609 | 7:-79679461 | None:intergenic |
| ATATCTAAAATAAGAAAATT+GGG | 0.431497 | 7:-79681006 | None:intergenic |
| CCAATTCCGGTTGGCGTCCG+TGG | 0.435135 | 7:+79679455 | MsG0780040594.01.T01:CDS |
| CTATGTGTCCTTCTTGTGAA+AGG | 0.437758 | 7:+79680802 | MsG0780040594.01.T01:CDS |
| GAGTCTGAGTTTCTTCCTTG+TGG | 0.443252 | 7:-79679643 | None:intergenic |
| AGAAAATAAAAGGTTACAAA+AGG | 0.465210 | 7:+79680710 | MsG0780040594.01.T01:CDS |
| GATGGTTTGTTTAGTGGCAG+TGG | 0.466617 | 7:-79680847 | None:intergenic |
| CACATAGTGAGAGTGGTAGC+TGG | 0.479169 | 7:-79680786 | None:intergenic |
| CAGAAACAGTAGTCGAGAAG+AGG | 0.481552 | 7:+79679581 | MsG0780040594.01.T01:CDS |
| GGCTGCAGTTGGTGTGGAAC+CGG | 0.481908 | 7:+79679413 | MsG0780040594.01.T01:CDS |
| GGCAGTGGTTCCGGTGGTGT+TGG | 0.486933 | 7:-79680832 | None:intergenic |
| CAAGTTAAAGCAAACGGAAG+TGG | 0.499846 | 7:+79680647 | MsG0780040594.01.T01:CDS |
| AGTGGTTCCGGTGGTGTTGG+TGG | 0.508654 | 7:-79680829 | None:intergenic |
| CCAGCGATGATGAAGAAAAT+GGG | 0.527273 | 7:+79679619 | MsG0780040594.01.T01:CDS |
| AGAACACACCACTCTCAATC+CGG | 0.532530 | 7:+79679704 | MsG0780040594.01.T01:CDS |
| CAGGACCAAGTTAAAGCAAA+CGG | 0.533115 | 7:+79680641 | MsG0780040594.01.T01:intron |
| TATGTGTCCTTCTTGTGAAA+GGG | 0.534150 | 7:+79680803 | MsG0780040594.01.T01:CDS |
| GCAGAAACAGAAACTTGCTC+TGG | 0.539316 | 7:+79680405 | MsG0780040594.01.T01:intron |
| AGAACCGTTGTCATTATTGG+CGG | 0.539567 | 7:-79680890 | None:intergenic |
| ATAGCCAAGGGCAATAATCT+GGG | 0.552582 | 7:-79680946 | None:intergenic |
| GAAGCCCAGATTATTGCCCT+TGG | 0.554740 | 7:+79680942 | MsG0780040594.01.T01:three_prime_UTR |
| GTGGCGACCCTTTCACAAGA+AGG | 0.556254 | 7:-79680810 | None:intergenic |
| TAAATTATAGGCTATAGCCA+AGG | 0.572931 | 7:-79680959 | None:intergenic |
| AGAAACAGTAGTCGAGAAGA+GGG | 0.577673 | 7:+79679582 | MsG0780040594.01.T01:CDS |
| CCCACCGGTGGTAATCGAGA+TGG | 0.582759 | 7:-79680865 | None:intergenic |
| AGGGTCGCCACCAACACCAC+CGG | 0.588362 | 7:+79680822 | MsG0780040594.01.T01:CDS |
| GCTGGAAGTTGCATGTAGAA+TGG | 0.591856 | 7:-79680768 | None:intergenic |
| ACCATCTCGATTACCACCGG+TGG | 0.592762 | 7:+79680864 | MsG0780040594.01.T01:CDS |
| TTCACATCCGATACTCTCAC+CGG | 0.595525 | 7:-79679432 | None:intergenic |
| AGAAGGACACATAGTGAGAG+TGG | 0.600200 | 7:-79680793 | None:intergenic |
| TGCAGCGTCATATTATCAGA+TGG | 0.603249 | 7:+79679521 | MsG0780040594.01.T01:CDS |
| TATTATTACCGGATTGAGAG+TGG | 0.604923 | 7:-79679712 | None:intergenic |
| AACCAAACTTCTACTTGACG+TGG | 0.605331 | 7:-79680448 | None:intergenic |
| TTATTGGCGGTGCCCACCGG+TGG | 0.607951 | 7:-79680877 | None:intergenic |
| GTTTACGGCTGCAGTTGGTG+TGG | 0.608602 | 7:+79679407 | MsG0780040594.01.T01:CDS |
| CAGTAGTCGAGAAGAGGGTG+CGG | 0.609350 | 7:+79679587 | MsG0780040594.01.T01:CDS |
| TGTATGTTTACGGCTGCAGT+TGG | 0.610027 | 7:+79679402 | MsG0780040594.01.T01:CDS |
| GGTTTCAGAATAGAAGAGCA+AGG | 0.612001 | 7:+79680467 | MsG0780040594.01.T01:CDS |
| ATGAAGAAAATGGGTCCACA+AGG | 0.617090 | 7:+79679628 | MsG0780040594.01.T01:CDS |
| AAATTATAGGCTATAGCCAA+GGG | 0.622732 | 7:-79680958 | None:intergenic |
| TGTGGAACCGGTGAGAGTAT+CGG | 0.632478 | 7:+79679425 | MsG0780040594.01.T01:CDS |
| ATTCCGGTTGGCGTCCGTGG+AGG | 0.636105 | 7:+79679458 | MsG0780040594.01.T01:CDS |
| ATATGACGCTGCACTATTCG+GGG | 0.636746 | 7:-79679511 | None:intergenic |
| CCATCTCGATTACCACCGGT+GGG | 0.643789 | 7:+79680865 | MsG0780040594.01.T01:CDS |
| CAAACCATCTCGATTACCAC+CGG | 0.647502 | 7:+79680861 | MsG0780040594.01.T01:CDS |
| TGAAACTGCAGCTCCCTCGT+CGG | 0.652204 | 7:-79679487 | None:intergenic |
| GGATGTGAACCAATTCCGGT+TGG | 0.657900 | 7:+79679446 | MsG0780040594.01.T01:CDS |
| TTTAGTGGCAGTGGTTCCGG+TGG | 0.663174 | 7:-79680838 | None:intergenic |
| GGCACCGCCAATAATGACAA+CGG | 0.678892 | 7:+79680886 | MsG0780040594.01.T01:exon |
| CTCGTCGGTAGTATCCTCCA+CGG | 0.686078 | 7:-79679472 | None:intergenic |
| CGTGGAGGATACTACCGACG+AGG | 0.692188 | 7:+79679473 | MsG0780040594.01.T01:CDS |
| GTGGAGGATACTACCGACGA+GGG | 0.757148 | 7:+79679474 | MsG0780040594.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATCTAAAATAAGAAAATT+GGG | - | Chr7:79681009-79681028 | None:intergenic | 10.0% |
| !!! | ATGTTTTTCATTTTTTTATT+AGG | - | Chr7:79680615-79680634 | None:intergenic | 10.0% |
| !!! | GAAATAAATATTGTTATTTT+AGG | + | Chr7:79680350-79680369 | MsG0780040594.01.T01:intron | 10.0% |
| !!! | TATATCTAAAATAAGAAAAT+TGG | - | Chr7:79681010-79681029 | None:intergenic | 10.0% |
| !! | AGTATAGTTACTTAAAAAAT+AGG | - | Chr7:79679376-79679395 | None:intergenic | 15.0% |
| !! | GTATAGTTACTTAAAAAATA+GGG | - | Chr7:79679375-79679394 | None:intergenic | 15.0% |
| !! | TAACTAAATAATCATTTAAC+TGG | - | Chr7:79680042-79680061 | None:intergenic | 15.0% |
| !! | TATAGTTACTTAAAAAATAG+GGG | - | Chr7:79679374-79679393 | None:intergenic | 15.0% |
| !! | TGAAAAATCTGATAAATTAT+AGG | - | Chr7:79680974-79680993 | None:intergenic | 15.0% |
| !! | AAATGAAAAACATAATGTAC+AGG | + | Chr7:79680622-79680641 | MsG0780040594.01.T01:intron | 20.0% |
| !! | AGAAAATAAAAGGTTACAAA+AGG | + | Chr7:79680710-79680729 | MsG0780040594.01.T01:CDS | 20.0% |
| !! | TACTTATATGTGTATGTTTA+CGG | + | Chr7:79679392-79679411 | MsG0780040594.01.T01:CDS | 20.0% |
| !! | TATAATATAAAAAATCGCAG+AGG | + | Chr7:79680234-79680253 | MsG0780040594.01.T01:intron | 20.0% |
| !!! | AAATAATTTGTGATAAGTGT+GGG | - | Chr7:79679926-79679945 | None:intergenic | 20.0% |
| !!! | ATCACAAATTATTTCTCTTT+TGG | + | Chr7:79679932-79679951 | MsG0780040594.01.T01:intron | 20.0% |
| !!! | TGTTTCTAAAAATTTTGTGT+AGG | - | Chr7:79679846-79679865 | None:intergenic | 20.0% |
| ! | AAATGAAATGTTGTTAGAGA+GGG | - | Chr7:79679741-79679760 | None:intergenic | 25.0% |
| ! | AGATATATGATGATATGTGT+GGG | - | Chr7:79679958-79679977 | None:intergenic | 25.0% |
| ! | CTCTAACAGAAGAAAATAAA+AGG | + | Chr7:79680700-79680719 | MsG0780040594.01.T01:CDS | 25.0% |
| !! | ATCCAGAATTTTTGAATAGT+GGG | - | Chr7:79680280-79680299 | None:intergenic | 25.0% |
| !! | ATGGATTTTTCTATCATGAA+TGG | + | Chr7:79679540-79679559 | MsG0780040594.01.T01:CDS | 25.0% |
| !! | ATTATCTTTTCTGTGTTATG+AGG | + | Chr7:79680503-79680522 | MsG0780040594.01.T01:intron | 25.0% |
| !! | CAGATTTTTCATGATATGAT+TGG | + | Chr7:79680983-79681002 | MsG0780040594.01.T01:three_prime_UTR | 25.0% |
| !! | GAAATAATTTGTGATAAGTG+TGG | - | Chr7:79679927-79679946 | None:intergenic | 25.0% |
| !! | GATTTTTATCATGCAATACA+TGG | + | Chr7:79679792-79679811 | MsG0780040594.01.T01:intron | 25.0% |
| !! | GCAGAGGATAATTTTATATA+TGG | + | Chr7:79680250-79680269 | MsG0780040594.01.T01:intron | 25.0% |
| !! | TTTTCTATCATGAATGGTAA+TGG | + | Chr7:79679546-79679565 | MsG0780040594.01.T01:CDS | 25.0% |
| AAATTATAGGCTATAGCCAA+GGG | - | Chr7:79680961-79680980 | None:intergenic | 30.0% | |
| ATAAGAACCGTTGTCATTAT+TGG | - | Chr7:79680896-79680915 | None:intergenic | 30.0% | |
| ATCAAATGTTAAATCGTGAG+AGG | + | Chr7:79680127-79680146 | MsG0780040594.01.T01:intron | 30.0% | |
| CAAATGAAATGTTGTTAGAG+AGG | - | Chr7:79679742-79679761 | None:intergenic | 30.0% | |
| GAGATATATGATGATATGTG+TGG | - | Chr7:79679959-79679978 | None:intergenic | 30.0% | |
| TAAATTATAGGCTATAGCCA+AGG | - | Chr7:79680962-79680981 | None:intergenic | 30.0% | |
| ! | GATCCAGAATTTTTGAATAG+TGG | - | Chr7:79680281-79680300 | None:intergenic | 30.0% |
| ! | TCCAGAATTTTTGAATAGTG+GGG | - | Chr7:79680279-79680298 | None:intergenic | 30.0% |
| ! | TTTTATCATGCAATACATGG+AGG | + | Chr7:79679795-79679814 | MsG0780040594.01.T01:intron | 30.0% |
| ATAGAAGAGCAAGGTAACTA+AGG | + | Chr7:79680476-79680495 | MsG0780040594.01.T01:intron | 35.0% | |
| ATCAAATGTGAAATCGTGAG+AGG | + | Chr7:79680013-79680032 | MsG0780040594.01.T01:intron | 35.0% | |
| TAATATGACGCTGCACTATT+CGG | - | Chr7:79679516-79679535 | None:intergenic | 35.0% | |
| TAGAAGAGCAAGGTAACTAA+GGG | + | Chr7:79680477-79680496 | MsG0780040594.01.T01:intron | 35.0% | |
| TATGTGTCCTTCTTGTGAAA+GGG | + | Chr7:79680803-79680822 | MsG0780040594.01.T01:CDS | 35.0% | |
| TATTATTACCGGATTGAGAG+TGG | - | Chr7:79679715-79679734 | None:intergenic | 35.0% | |
| ! | CCAGAATTTTTGAATAGTGG+GGG | - | Chr7:79680278-79680297 | None:intergenic | 35.0% |
| !! | AATCGAGATGGTTTGTTTAG+TGG | - | Chr7:79680856-79680875 | None:intergenic | 35.0% |
| !! | AGAGAGGGTGTTATTATTAC+CGG | - | Chr7:79679726-79679745 | None:intergenic | 35.0% |
| !! | AGTTTCTGTTTCTGCAGATT+TGG | - | Chr7:79680400-79680419 | None:intergenic | 35.0% |
| !! | GTTTCTGTTTCTGCAGATTT+GGG | - | Chr7:79680399-79680418 | None:intergenic | 35.0% |
| AACCAAACTTCTACTTGACG+TGG | - | Chr7:79680451-79680470 | None:intergenic | 40.0% | |
| AATATGACGCTGCACTATTC+GGG | - | Chr7:79679515-79679534 | None:intergenic | 40.0% | |
| ACCAGCGATGATGAAGAAAA+TGG | + | Chr7:79679618-79679637 | MsG0780040594.01.T01:CDS | 40.0% | |
| AGAAACAGTAGTCGAGAAGA+GGG | + | Chr7:79679582-79679601 | MsG0780040594.01.T01:CDS | 40.0% | |
| AGAACCGTTGTCATTATTGG+CGG | - | Chr7:79680893-79680912 | None:intergenic | 40.0% | |
| ATAGCCAAGGGCAATAATCT+GGG | - | Chr7:79680949-79680968 | None:intergenic | 40.0% | |
| ATGAAGAAAATGGGTCCACA+AGG | + | Chr7:79679628-79679647 | MsG0780040594.01.T01:CDS | 40.0% | |
| ATGGAGGAAAAACCTTCAAG+TGG | + | Chr7:79679811-79679830 | MsG0780040594.01.T01:intron | 40.0% | |
| CAAGTTAAAGCAAACGGAAG+TGG | + | Chr7:79680647-79680666 | MsG0780040594.01.T01:CDS | 40.0% | |
| CACTTCCGTTTGCTTTAACT+TGG | - | Chr7:79680649-79680668 | None:intergenic | 40.0% | |
| CAGGACCAAGTTAAAGCAAA+CGG | + | Chr7:79680641-79680660 | MsG0780040594.01.T01:intron | 40.0% | |
| CCAGCGATGATGAAGAAAAT+GGG | + | Chr7:79679619-79679638 | MsG0780040594.01.T01:CDS | 40.0% | |
| CCCCCACTATTCAAAAATTC+TGG | + | Chr7:79680275-79680294 | MsG0780040594.01.T01:intron | 40.0% | |
| CTATGTGTCCTTCTTGTGAA+AGG | + | Chr7:79680802-79680821 | MsG0780040594.01.T01:CDS | 40.0% | |
| GAAGAAACTCAGACTCTCTA+AGG | + | Chr7:79679650-79679669 | MsG0780040594.01.T01:CDS | 40.0% | |
| GGTTTCAGAATAGAAGAGCA+AGG | + | Chr7:79680467-79680486 | MsG0780040594.01.T01:CDS | 40.0% | |
| TATAGCCAAGGGCAATAATC+TGG | - | Chr7:79680950-79680969 | None:intergenic | 40.0% | |
| TATCGGATGTGAACCAATTC+CGG | + | Chr7:79679442-79679461 | MsG0780040594.01.T01:CDS | 40.0% | |
| TGCAGCGTCATATTATCAGA+TGG | + | Chr7:79679521-79679540 | MsG0780040594.01.T01:CDS | 40.0% | |
| TGGAGGAAAAACCTTCAAGT+GGG | + | Chr7:79679812-79679831 | MsG0780040594.01.T01:intron | 40.0% | |
| TGTGATAAGTGTGGGTCATA+TGG | - | Chr7:79679918-79679937 | None:intergenic | 40.0% | |
| AGAACACACCACTCTCAATC+CGG | + | Chr7:79679704-79679723 | MsG0780040594.01.T01:CDS | 45.0% | |
| AGAAGGACACATAGTGAGAG+TGG | - | Chr7:79680796-79680815 | None:intergenic | 45.0% | |
| ATATGACGCTGCACTATTCG+GGG | - | Chr7:79679514-79679533 | None:intergenic | 45.0% | |
| CAAACCATCTCGATTACCAC+CGG | + | Chr7:79680861-79680880 | MsG0780040594.01.T01:CDS | 45.0% | |
| CAGAAACAGTAGTCGAGAAG+AGG | + | Chr7:79679581-79679600 | MsG0780040594.01.T01:CDS | 45.0% | |
| CTCTTCTCGACTACTGTTTC+TGG | - | Chr7:79679583-79679602 | None:intergenic | 45.0% | |
| GAGGAAAAACCTTCAAGTGG+GGG | + | Chr7:79679814-79679833 | MsG0780040594.01.T01:intron | 45.0% | |
| GAGTCTGAGTTTCTTCCTTG+TGG | - | Chr7:79679646-79679665 | None:intergenic | 45.0% | |
| GCTGGAAGTTGCATGTAGAA+TGG | - | Chr7:79680771-79680790 | None:intergenic | 45.0% | |
| GGAGGAAAAACCTTCAAGTG+GGG | + | Chr7:79679813-79679832 | MsG0780040594.01.T01:intron | 45.0% | |
| GTCCACGTCAAGTAGAAGTT+TGG | + | Chr7:79680446-79680465 | MsG0780040594.01.T01:CDS | 45.0% | |
| TGTATGTTTACGGCTGCAGT+TGG | + | Chr7:79679402-79679421 | MsG0780040594.01.T01:CDS | 45.0% | |
| TTCACATCCGATACTCTCAC+CGG | - | Chr7:79679435-79679454 | None:intergenic | 45.0% | |
| TTGTTTAGTGGCAGTGGTTC+CGG | - | Chr7:79680844-79680863 | None:intergenic | 45.0% | |
| ! | CCCATTTTCTTCATCATCGC+TGG | - | Chr7:79679622-79679641 | None:intergenic | 45.0% |
| ! | GCAGAAACAGAAACTTGCTC+TGG | + | Chr7:79680405-79680424 | MsG0780040594.01.T01:intron | 45.0% |
| !! | GATGGTTTGTTTAGTGGCAG+TGG | - | Chr7:79680850-79680869 | None:intergenic | 45.0% |
| !! | AAATAAACAAAATAAATATT+TGG | - | Chr7:79680549-79680568 | None:intergenic | 5.0% |
| CACATAGTGAGAGTGGTAGC+TGG | - | Chr7:79680789-79680808 | None:intergenic | 50.0% | |
| GAAGCCCAGATTATTGCCCT+TGG | + | Chr7:79680942-79680961 | MsG0780040594.01.T01:three_prime_UTR | 50.0% | |
| GGATGTGAACCAATTCCGGT+TGG | + | Chr7:79679446-79679465 | MsG0780040594.01.T01:CDS | 50.0% | |
| GGCACCGCCAATAATGACAA+CGG | + | Chr7:79680886-79680905 | MsG0780040594.01.T01:exon | 50.0% | |
| ! | TGTGGAACCGGTGAGAGTAT+CGG | + | Chr7:79679425-79679444 | MsG0780040594.01.T01:CDS | 50.0% |
| ACCATCTCGATTACCACCGG+TGG | + | Chr7:79680864-79680883 | MsG0780040594.01.T01:CDS | 55.0% | |
| CAGTAGTCGAGAAGAGGGTG+CGG | + | Chr7:79679587-79679606 | MsG0780040594.01.T01:CDS | 55.0% | |
| CCATCTCGATTACCACCGGT+GGG | + | Chr7:79680865-79680884 | MsG0780040594.01.T01:CDS | 55.0% | |
| CTCGTCGGTAGTATCCTCCA+CGG | - | Chr7:79679475-79679494 | None:intergenic | 55.0% | |
| GTGGAGGATACTACCGACGA+GGG | + | Chr7:79679474-79679493 | MsG0780040594.01.T01:CDS | 55.0% | |
| GTGGCGACCCTTTCACAAGA+AGG | - | Chr7:79680813-79680832 | None:intergenic | 55.0% | |
| TCATTATTGGCGGTGCCCAC+CGG | - | Chr7:79680883-79680902 | None:intergenic | 55.0% | |
| TGAAACTGCAGCTCCCTCGT+CGG | - | Chr7:79679490-79679509 | None:intergenic | 55.0% | |
| TTTAGTGGCAGTGGTTCCGG+TGG | - | Chr7:79680841-79680860 | None:intergenic | 55.0% | |
| ! | GTTTACGGCTGCAGTTGGTG+TGG | + | Chr7:79679407-79679426 | MsG0780040594.01.T01:CDS | 55.0% |
| AGGCACAAGCCCCCACTTGA+AGG | - | Chr7:79679826-79679845 | None:intergenic | 60.0% | |
| CCCACCGGTGGTAATCGAGA+TGG | - | Chr7:79680868-79680887 | None:intergenic | 60.0% | |
| CGTGGAGGATACTACCGACG+AGG | + | Chr7:79679473-79679492 | MsG0780040594.01.T01:CDS | 60.0% | |
| TATCCTCCACGGACGCCAAC+CGG | - | Chr7:79679464-79679483 | None:intergenic | 60.0% | |
| ! | AGTGGTTCCGGTGGTGTTGG+TGG | - | Chr7:79680832-79680851 | None:intergenic | 60.0% |
| ! | GGCTGCAGTTGGTGTGGAAC+CGG | + | Chr7:79679413-79679432 | MsG0780040594.01.T01:CDS | 60.0% |
| AGGGTCGCCACCAACACCAC+CGG | + | Chr7:79680822-79680841 | MsG0780040594.01.T01:CDS | 65.0% | |
| ATTCCGGTTGGCGTCCGTGG+AGG | + | Chr7:79679458-79679477 | MsG0780040594.01.T01:CDS | 65.0% | |
| CCAATTCCGGTTGGCGTCCG+TGG | + | Chr7:79679455-79679474 | MsG0780040594.01.T01:CDS | 65.0% | |
| CCACGGACGCCAACCGGAAT+TGG | - | Chr7:79679458-79679477 | None:intergenic | 65.0% | |
| GGCAGTGGTTCCGGTGGTGT+TGG | - | Chr7:79680835-79680854 | None:intergenic | 65.0% | |
| TTATTGGCGGTGCCCACCGG+TGG | - | Chr7:79680880-79680899 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 79679330 | 79681060 | 79679330 | ID=MsG0780040594.01;Name=MsG0780040594.01 |
| Chr7 | mRNA | 79679330 | 79681060 | 79679330 | ID=MsG0780040594.01.T01;Parent=MsG0780040594.01;Name=MsG0780040594.01.T01;_AED=0.32;_eAED=0.33;_QI=0|1|0.66|1|1|1|3|161|243 |
| Chr7 | exon | 79679330 | 79679725 | 79679330 | ID=MsG0780040594.01.T01:exon:16540;Parent=MsG0780040594.01.T01 |
| Chr7 | exon | 79680409 | 79680488 | 79680409 | ID=MsG0780040594.01.T01:exon:16541;Parent=MsG0780040594.01.T01 |
| Chr7 | exon | 79680644 | 79681060 | 79680644 | ID=MsG0780040594.01.T01:exon:16542;Parent=MsG0780040594.01.T01 |
| Chr7 | CDS | 79679330 | 79679725 | 79679330 | ID=MsG0780040594.01.T01:cds;Parent=MsG0780040594.01.T01 |
| Chr7 | CDS | 79680409 | 79680488 | 79680409 | ID=MsG0780040594.01.T01:cds;Parent=MsG0780040594.01.T01 |
| Chr7 | CDS | 79680644 | 79680899 | 79680644 | ID=MsG0780040594.01.T01:cds;Parent=MsG0780040594.01.T01 |
| Chr7 | three_prime_UTR | 79680900 | 79681060 | 79680900 | ID=MsG0780040594.01.T01:three_prime_utr;Parent=MsG0780040594.01.T01 |
| Gene Sequence |
| Protein sequence |
