AlfalfaGEDB | MsG0880045015.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045015.01

KEGG: K11253
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045015.01.T01	KYP58465.1	100	136	0	0	1	136	5	140	1.76E-92	273

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045015.01.T01	P68427	100	136	0	0	1	136	1	136	5.13E-95	272

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045015.01.T01	A0A452Z7H5	100.000	136	0	0	1	136	100	235	3.73e-93	278

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0880045015.01	0.813258	2.803374e-51	1.821687e-48
MsG0080048258.01	MsG0880045015.01	0.805538	1.272171e-49	6.747525e-47
MsG0080048781.01	MsG0880045015.01	0.813380	2.635998e-51	1.718760e-48
MsG0080048797.01	MsG0880045015.01	0.811269	7.617385e-51	4.694168e-48
MsG0180001293.01	MsG0880045015.01	0.800186	1.621976e-48	7.504089e-46
MsG0180001588.01	MsG0880045015.01	0.840429	8.744267e-58	1.245189e-54
MsG0180001719.01	MsG0880045015.01	0.821416	4.085990e-53	3.322122e-50
MsG0180002218.01	MsG0880045015.01	0.827347	1.644960e-54	1.584060e-51
MsG0880045015.01	MsG0880045356.01	0.810532	1.099794e-50	6.645904e-48
MsG0880045015.01	MsG0880045612.01	0.818335	2.068492e-52	1.543979e-49
MsG0880045015.01	MsG0880045647.01	0.844392	7.776174e-59	1.253526e-55
MsG0880045015.01	MsG0880046431.01	0.813821	2.107743e-51	1.390821e-48
MsG0880045015.01	MsG0880046529.01	0.821944	3.083552e-53	2.544738e-50
MsG0880045015.01	MsG0880046904.01	0.832846	7.485383e-56	8.468720e-53
MsG0880045015.01	MsG0880046905.01	0.850559	1.570705e-60	3.088830e-57
MsG0880045015.01	MsG0880047649.01	0.830936	2.217664e-55	2.371045e-52
MsG0180002936.01	MsG0880045015.01	0.817569	3.081047e-52	2.251779e-49
MsG0180003421.01	MsG0880045015.01	0.818354	2.048139e-52	1.529655e-49
MsG0180003857.01	MsG0880045015.01	0.812736	3.647912e-51	2.337759e-48
MsG0180003905.01	MsG0880045015.01	0.816728	4.762812e-52	3.401585e-49
MsG0180004120.01	MsG0880045015.01	0.901168	3.519488e-78	4.697355e-74
MsG0180004185.01	MsG0880045015.01	0.804088	2.555407e-49	1.305778e-46
MsG0180004471.01	MsG0880045015.01	0.830786	2.413571e-55	2.569088e-52
MsG0180004475.01	MsG0880045015.01	0.839033	2.018615e-57	2.753127e-54
MsG0180004538.01	MsG0880045015.01	0.858952	5.784598e-63	1.502393e-59
MsG0180004583.01	MsG0880045015.01	0.852492	4.456791e-61	9.328584e-58
MsG0180004661.01	MsG0880045015.01	0.802803	4.717568e-49	2.332570e-46
MsG0180004972.01	MsG0880045015.01	0.809582	1.762307e-50	1.038728e-47
MsG0180004994.01	MsG0880045015.01	0.842676	2.235455e-58	3.414595e-55
MsG0180004995.01	MsG0880045015.01	0.865726	4.790489e-65	1.574711e-61
MsG0180005048.01	MsG0880045015.01	0.908341	1.900243e-81	3.512336e-77
MsG0180005608.01	MsG0880045015.01	0.808920	2.442955e-50	1.414546e-47
MsG0180006062.01	MsG0880045015.01	0.809820	1.566192e-50	9.289325e-48
MsG0180006133.01	MsG0880045015.01	0.900459	7.172313e-78	9.285551e-74
MsG0780040594.01	MsG0880045015.01	0.811533	6.674508e-51	4.142078e-48
MsG0780040620.01	MsG0880045015.01	0.854102	1.539111e-61	3.400089e-58
MsG0780040641.01	MsG0880045015.01	0.838920	2.158949e-57	2.934329e-54
MsG0780040899.01	MsG0880045015.01	0.826417	2.744628e-54	2.572523e-51
MsG0780041008.01	MsG0880045015.01	0.811717	6.087611e-51	3.796098e-48
MsG0780041273.01	MsG0880045015.01	0.905131	5.931005e-80	9.462487e-76
MsG0780041359.01	MsG0880045015.01	0.830400	2.999769e-55	3.156963e-52
MsG0780041546.01	MsG0880045015.01	0.865714	4.832361e-65	1.587776e-61
MsG0880043684.01	MsG0880045015.01	0.801589	8.384854e-49	4.019446e-46
MsG0880043907.01	MsG0880045015.01	0.810146	1.332770e-50	7.972180e-48
MsG0380012956.01	MsG0880045015.01	0.809830	1.558428e-50	9.245718e-48
MsG0380014542.01	MsG0880045015.01	0.835767	1.385109e-56	1.710546e-53
MsG0380014666.01	MsG0880045015.01	0.885847	5.713365e-72	3.984103e-68
MsG0480018785.01	MsG0880045015.01	0.806322	8.701584e-50	4.709062e-47
MsG0480019601.01	MsG0880045015.01	0.800464	1.423578e-48	6.632743e-46
MsG0480020366.01	MsG0880045015.01	0.807017	6.206232e-50	3.419148e-47
MsG0480020398.01	MsG0880045015.01	0.871609	5.999526e-67	2.435447e-63
MsG0480020856.01	MsG0880045015.01	0.801196	1.009057e-48	4.789611e-46
MsG0480020920.01	MsG0880045015.01	0.804031	2.625535e-49	1.339669e-46
MsG0480020921.01	MsG0880045015.01	0.844213	8.690646e-59	1.393222e-55
MsG0480020926.01	MsG0880045015.01	0.853890	1.771122e-61	3.885046e-58
MsG0480020937.01	MsG0880045015.01	0.825127	5.552120e-54	5.016212e-51
MsG0480020969.01	MsG0880045015.01	0.802272	6.070850e-49	2.961240e-46
MsG0480020970.01	MsG0880045015.01	0.836735	7.860026e-57	9.994411e-54
MsG0480020971.01	MsG0880045015.01	0.807758	4.323150e-50	2.428025e-47
MsG0480021015.01	MsG0880045015.01	0.867768	1.072441e-65	3.788052e-62
MsG0480021016.01	MsG0880045015.01	0.807290	5.432667e-50	3.014353e-47
MsG0480021048.01	MsG0880045015.01	0.831043	2.087970e-55	2.239330e-52
MsG0480021100.01	MsG0880045015.01	0.861429	1.032780e-63	2.921827e-60
MsG0480021301.01	MsG0880045015.01	0.925553	1.568761e-90	7.035800e-86
MsG0480021302.01	MsG0880045015.01	0.854000	1.646941e-61	3.625692e-58
MsG0480021704.01	MsG0880045015.01	0.850947	1.221442e-60	2.433018e-57
MsG0480021773.01	MsG0880045015.01	0.844360	7.933216e-59	1.277557e-55
MsG0480021902.01	MsG0880045015.01	0.840960	6.347002e-58	9.188269e-55
MsG0480022307.01	MsG0880045015.01	0.912081	2.928672e-83	6.469089e-79
MsG0480022320.01	MsG0880045015.01	0.818178	2.244136e-52	1.667846e-49
MsG0480022378.01	MsG0880045015.01	0.804550	2.047159e-49	1.058534e-46
MsG0480022784.01	MsG0880045015.01	0.827417	1.582471e-54	1.526918e-51
MsG0480023003.01	MsG0880045015.01	0.807363	5.242634e-50	2.914331e-47
MsG0480023424.01	MsG0880045015.01	0.874474	6.568549e-68	2.961096e-64
MsG0480023426.01	MsG0880045015.01	0.890917	6.382316e-74	5.475749e-70
MsG0480023428.01	MsG0880045015.01	0.820794	5.680750e-53	4.539554e-50
MsG0480023626.01	MsG0880045015.01	0.818845	1.584890e-52	1.199808e-49
MsG0480023653.01	MsG0880045015.01	0.826622	2.451578e-54	2.311194e-51
MsG0480023706.01	MsG0880045015.01	0.861825	7.811753e-64	2.239172e-60
MsG0480023842.01	MsG0880045015.01	0.854928	8.875563e-62	2.014608e-58
MsG0480024002.01	MsG0880045015.01	0.853222	2.755577e-61	5.911644e-58
MsG0480024019.01	MsG0880045015.01	0.819782	9.695324e-53	7.533076e-50
MsG0580024158.01	MsG0880045015.01	0.818336	2.067326e-52	1.543161e-49
MsG0580024276.01	MsG0880045015.01	0.845365	4.251557e-59	7.068245e-56
MsG0580024388.01	MsG0880045015.01	0.821731	3.453930e-53	2.833200e-50
MsG0580024787.01	MsG0880045015.01	0.833290	5.804220e-56	6.654718e-53
MsG0580024954.01	MsG0880045015.01	0.822018	2.965333e-53	2.452203e-50
MsG0580025038.01	MsG0880045015.01	0.822676	2.085412e-53	1.757401e-50
MsG0580025177.01	MsG0880045015.01	0.835295	1.823102e-56	2.220183e-53
MsG0580025203.01	MsG0880045015.01	0.841771	3.882885e-58	5.764702e-55
MsG0580025504.01	MsG0880045015.01	0.904549	1.092907e-79	1.699580e-75
MsG0580025506.01	MsG0880045015.01	0.856421	3.253899e-62	7.762701e-59
MsG0380015090.01	MsG0880045015.01	0.821067	4.917778e-53	3.960153e-50
MsG0380015219.01	MsG0880045015.01	0.815850	7.482264e-52	5.216964e-49
MsG0380015340.01	MsG0880045015.01	0.812294	4.557723e-51	2.886765e-48
MsG0380015402.01	MsG0880045015.01	0.847013	1.512124e-59	2.651068e-56
MsG0380016027.01	MsG0880045015.01	0.845039	5.206329e-59	8.566650e-56
MsG0380016091.01	MsG0880045015.01	0.831577	1.542358e-55	1.680855e-52
MsG0380016225.01	MsG0880045015.01	0.872761	2.481079e-67	1.050573e-63
MsG0380017128.01	MsG0880045015.01	0.807049	6.110255e-50	3.369049e-47
MsG0380017181.01	MsG0880045015.01	0.824040	1.001361e-53	8.768984e-51
MsG0380017282.01	MsG0880045015.01	0.843287	1.537627e-58	2.394473e-55
MsG0380017332.01	MsG0880045015.01	0.850395	1.745717e-60	3.414858e-57
MsG0380017588.01	MsG0880045015.01	0.826409	2.756985e-54	2.583485e-51
MsG0380017605.01	MsG0880045015.01	0.837913	3.925969e-57	5.174752e-54
MsG0380017696.01	MsG0880045015.01	0.850282	1.878050e-60	3.659831e-57
MsG0380017932.01	MsG0880045015.01	0.820172	7.893850e-53	6.200462e-50
MsG0480018106.01	MsG0880045015.01	0.826217	3.062794e-54	2.854330e-51
MsG0480018228.01	MsG0880045015.01	0.836730	7.884351e-57	1.002369e-53
MsG0580028633.01	MsG0880045015.01	0.819991	8.684781e-53	6.787194e-50
MsG0580028927.01	MsG0880045015.01	0.865179	7.124361e-65	2.296781e-61
MsG0580028955.01	MsG0880045015.01	0.893592	5.447225e-75	5.222872e-71
MsG0580029591.01	MsG0880045015.01	0.852497	4.442335e-61	9.300186e-58
MsG0280006717.01	MsG0880045015.01	0.814109	1.821105e-51	1.211072e-48
MsG0280007003.01	MsG0880045015.01	0.800243	1.579470e-48	7.318030e-46
MsG0280007092.01	MsG0880045015.01	0.806380	8.461548e-50	4.585809e-47
MsG0280007492.01	MsG0880045015.01	0.828654	7.970824e-55	7.972788e-52
MsG0280007919.01	MsG0880045015.01	0.879830	9.042605e-70	4.977460e-66
MsG0280009866.01	MsG0880045015.01	0.804601	1.997192e-49	1.034096e-46
MsG0680035776.01	MsG0880045015.01	0.837581	4.778003e-57	6.234032e-54
MsG0680035792.01	MsG0880045015.01	0.864807	9.318805e-65	2.964076e-61
MsG0780036534.01	MsG0880045015.01	0.822390	2.430543e-53	2.031711e-50
MsG0780038642.01	MsG0880045015.01	0.833436	5.337885e-56	6.147119e-53
MsG0780038736.01	MsG0880045015.01	0.833999	3.861196e-56	4.522395e-53
MsG0780039536.01	MsG0880045015.01	0.802000	6.905695e-49	3.345110e-46

PPI

Gene1	Gene2	Type
MsG0880044910.01	MsG0880045015.01	PPI
MsG0180000092.01	MsG0880045015.01	PPI
MsG0480020919.01	MsG0880045015.01	PPI
MsG0780038642.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0380016970.01	PPI
MsG0880045015.01	MsG0380018077.01	PPI
MsG0880045015.01	MsG0780041592.01	PPI
MsG0880045015.01	MsG0680034619.01	PPI
MsG0880045015.01	MsG0480021302.01	PPI
MsG0880045015.01	MsG0880044881.01	PPI
MsG0380016970.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0480023842.01	PPI
MsG0880045015.01	MsG0480022796.01	PPI
MsG0480021049.01	MsG0880045015.01	PPI
MsG0480020918.01	MsG0880045015.01	PPI
MsG0380017977.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0780041593.01	PPI
MsG0880045015.01	MsG0780036534.01	PPI
MsG0880045015.01	MsG0880044910.01	PPI
MsG0880045015.01	MsG0480022307.01	PPI
MsG0880045996.01	MsG0880045015.01	PPI
MsG0480020969.01	MsG0880045015.01	PPI
MsG0580029044.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0480020920.01	PPI
MsG0880045015.01	MsG0380012103.01	PPI
MsG0480021015.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0780039858.01	PPI
MsG0880045015.01	MsG0480020927.01	PPI
MsG0580025506.01	MsG0880045015.01	PPI
MsG0880045015.01	MsG0180000092.01	PPI
MsG0880045015.01	MsG0280007919.01	PPI
MsG0880045015.01	MsG0580025504.01	PPI
MsG0880045015.01	MsG0580029382.01	PPI
MsG0880045015.01	MsG0480021252.01	PPI
MsG0880045015.01	MsG0880046700.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045015.01.T01	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.99e-96	272
MsG0880045015.01.T01	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.15e-95	275
MsG0880045015.01.T01	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.42e-93	265
MsG0880045015.01.T01	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.42e-93	265
MsG0880045015.01.T01	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.42e-93	265
MsG0880045015.01.T01	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.44e-92	264
MsG0880045015.01.T01	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.38e-91	262
MsG0880045015.01.T01	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.44e-86	248
MsG0880045015.01.T01	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.44e-86	248
MsG0880045015.01.T01	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.82e-82	238
MsG0880045015.01.T01	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.64e-75	219
MsG0880045015.01.T01	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.05e-65	195
MsG0880045015.01.T01	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.74e-56	171
MsG0880045015.01.T01	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.96e-52	160
MsG0880045015.01.T01	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.69e-45	144
MsG0880045015.01.T01	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.67e-43	139
MsG0880045015.01.T01	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.74e-42	137
MsG0880045015.01.T01	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.50e-40	132
MsG0880045015.01.T01	MTR_8g027840	55.102	98	43	1	37	133	28	125	5.01e-30	105
MsG0880045015.01.T01	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.48e-25	92.4
MsG0880045015.01.T01	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.75e-20	80.9
MsG0880045015.01.T01	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.26e-18	73.6
MsG0880045015.01.T01	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.81e-15	66.2
MsG0880045015.01.T01	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.48e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045015.01.T01	AT5G65360	99.265	136	1	0	1	136	1	136	1.62e-95	271
MsG0880045015.01.T01	AT5G10390	99.265	136	1	0	1	136	1	136	1.62e-95	271
MsG0880045015.01.T01	AT5G10400	99.265	136	1	0	1	136	1	136	1.62e-95	271
MsG0880045015.01.T01	AT3G27360	99.265	136	1	0	1	136	1	136	1.62e-95	271
MsG0880045015.01.T01	AT1G09200	99.265	136	1	0	1	136	1	136	1.62e-95	271
MsG0880045015.01.T01	AT5G10980	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40040	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40040	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40040	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40030	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40030	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40030	97.059	136	4	0	1	136	1	136	3.59e-93	265
MsG0880045015.01.T01	AT4G40030	97.059	136	4	0	1	136	29	164	7.04e-93	266
MsG0880045015.01.T01	AT5G65350	94.853	136	7	0	1	136	1	136	5.30e-91	260
MsG0880045015.01.T01	AT1G75600	93.382	136	9	0	1	136	1	136	1.25e-89	256
MsG0880045015.01.T01	AT1G13370	93.382	136	9	0	1	136	1	136	5.55e-89	254
MsG0880045015.01.T01	AT1G19890	89.781	137	13	1	1	136	1	137	1.07e-83	241
MsG0880045015.01.T01	AT5G12910	71.111	135	34	2	1	135	1	130	9.89e-67	198
MsG0880045015.01.T01	AT1G01370	53.982	113	49	2	24	134	63	174	2.97e-31	110
MsG0880045015.01.T01	AT1G01370	53.982	113	49	2	24	134	63	174	2.97e-31	110
MsG0880045015.01.T01	AT1G01370	53.982	113	49	2	24	134	63	174	2.97e-31	110
MsG0880045015.01.T01	AT1G01370	53.982	113	49	2	24	134	63	174	2.97e-31	110

Find 44 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAGAAGCCACACCGTTTC+AGG	0.224781	8:+52776425	MsG0880045015.01.T01:CDS
TCACGGAGTGCGACGGTTCC+TGG	0.249130	8:-52776448	None:intergenic
TGAGTAGTTCGGTGCTCTTC+TGG	0.344215	8:-52776483	None:intergenic
GCGCCGACACGGCAGAGCTC+TGG	0.363108	8:-52776573	None:intergenic
GCGGCGGAGGCTTATCTTGT+TGG	0.386513	8:+52776604	MsG0880045015.01.T01:CDS
CTTCCTTGGTGCTTTGCCGC+CGG	0.391014	8:-52776353	None:intergenic
CGCTTCTTGCAGCGCCGACA+CGG	0.409261	8:-52776584	None:intergenic
TTCTTCACTCCGCCGGTCGC+CGG	0.410833	8:-52776409	None:intergenic
CGGAAATCCGCTCCGGCGAC+CGG	0.410851	8:+52776397	MsG0880045015.01.T01:CDS
TTTGTTGCGAGTTGCTTCCT+TGG	0.418169	8:-52776367	None:intergenic
CCTTGGTGCTTTGCCGCCGG+TGG	0.443251	8:-52776350	None:intergenic
TTATCTTGTTGGATTGTTTG+AGG	0.444671	8:+52776615	MsG0880045015.01.T01:CDS
GGCGCTGCAAGAAGCGGCGG+AGG	0.459160	8:+52776591	MsG0880045015.01.T01:CDS
CGACGGTTCCTGGCCTGAAA+CGG	0.480580	8:-52776438	None:intergenic
GACGCTGGAAAGGAAGCTTC+CGG	0.481198	8:-52776507	None:intergenic
CTAGCAAGCTGGATATCCTT+AGG	0.481336	8:-52776682	None:intergenic
TCTTGTTCGTGAGATTGCTC+AGG	0.500503	8:+52776528	MsG0880045015.01.T01:CDS
CTAAGGATATCCAGCTTGCT+AGG	0.501049	8:+52776683	MsG0880045015.01.T01:CDS
AAGCCACACCGTTTCAGGCC+AGG	0.512079	8:+52776430	MsG0880045015.01.T01:CDS
ACACGGCAGAGCTCTGGAAA+CGG	0.525377	8:-52776567	None:intergenic
AAGCTTCCGGATGAGTAGTT+CGG	0.529555	8:-52776494	None:intergenic
CACTCCGCCGGTCGCCGGAG+CGG	0.534210	8:-52776404	None:intergenic
CGTGTCGGCGCTGCAAGAAG+CGG	0.535952	8:+52776585	MsG0880045015.01.T01:CDS
CACGGATACGCCTAGCAAGC+TGG	0.537953	8:-52776693	None:intergenic
GTGTGGCTTCTTCACTCCGC+CGG	0.542673	8:-52776416	None:intergenic
AACTCGCAACAAAAGCTGCT+CGG	0.545965	8:+52776377	MsG0880045015.01.T01:CDS
CGGTGCTCTTCTGGTACTTG+CGG	0.546004	8:-52776474	None:intergenic
GCGTGTTACTATCATGCCTA+AGG	0.550012	8:+52776666	MsG0880045015.01.T01:CDS
TTTCCAGAGCTCTGCCGTGT+CGG	0.550048	8:+52776570	MsG0880045015.01.T01:CDS
AGCTGCTCGGAAATCCGCTC+CGG	0.552840	8:+52776390	MsG0880045015.01.T01:CDS
TCTGGTACTTGCGGATCTCA+CGG	0.559013	8:-52776465	None:intergenic
AGAGCACCGAACTACTCATC+CGG	0.565108	8:+52776488	MsG0880045015.01.T01:CDS
AAATCCGCTCCGGCGACCGG+CGG	0.570605	8:+52776400	MsG0880045015.01.T01:CDS
CAAACTGCACGCAAATCCAC+CGG	0.574254	8:+52776334	MsG0880045015.01.T01:CDS
TCACGAACAAGACGCTGGAA+AGG	0.582899	8:-52776517	None:intergenic
CAATCTCACGAACAAGACGC+TGG	0.597333	8:-52776522	None:intergenic
GTCGGCGCTGCAAGAAGCGG+CGG	0.602488	8:+52776588	MsG0880045015.01.T01:CDS
AAGATCAAGCACGCTCGCCA+CGG	0.626947	8:-52776711	None:intergenic
CCACCGGCGGCAAAGCACCA+AGG	0.632902	8:+52776350	MsG0880045015.01.T01:CDS
GCGGATCTCACGGAGTGCGA+CGG	0.634415	8:-52776455	None:intergenic
GATTGCTCAGGATTTCAAGA+CGG	0.652525	8:+52776540	MsG0880045015.01.T01:CDS
GTTCCTGGCCTGAAACGGTG+TGG	0.654320	8:-52776433	None:intergenic
ACTGCACGCAAATCCACCGG+CGG	0.661692	8:+52776337	MsG0880045015.01.T01:CDS
CAGCTTGCTAGGCGTATCCG+TGG	0.666195	8:+52776694	MsG0880045015.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTATCTTGTTGGATTGTTTG+AGG	+	Chr8:52776615-52776634	MsG0880045015.01.T01:CDS	30.0%
	GATTGCTCAGGATTTCAAGA+CGG	+	Chr8:52776540-52776559	MsG0880045015.01.T01:CDS	40.0%
	AACTCGCAACAAAAGCTGCT+CGG	+	Chr8:52776377-52776396	MsG0880045015.01.T01:CDS	45.0%
	AAGCTTCCGGATGAGTAGTT+CGG	-	Chr8:52776497-52776516	None:intergenic	45.0%
	CTAGCAAGCTGGATATCCTT+AGG	-	Chr8:52776685-52776704	None:intergenic	45.0%
	TCTTGTTCGTGAGATTGCTC+AGG	+	Chr8:52776528-52776547	MsG0880045015.01.T01:CDS	45.0%
	TTTGTTGCGAGTTGCTTCCT+TGG	-	Chr8:52776370-52776389	None:intergenic	45.0%
!	CTAAGGATATCCAGCTTGCT+AGG	+	Chr8:52776683-52776702	MsG0880045015.01.T01:CDS	45.0%
!	GCGTGTTACTATCATGCCTA+AGG	+	Chr8:52776666-52776685	MsG0880045015.01.T01:CDS	45.0%
	CAAACTGCACGCAAATCCAC+CGG	+	Chr8:52776334-52776353	MsG0880045015.01.T01:CDS	50.0%
	CAATCTCACGAACAAGACGC+TGG	-	Chr8:52776525-52776544	None:intergenic	50.0%
	TCACGAACAAGACGCTGGAA+AGG	-	Chr8:52776520-52776539	None:intergenic	50.0%
	TCTGGTACTTGCGGATCTCA+CGG	-	Chr8:52776468-52776487	None:intergenic	50.0%
	TGAAGAAGCCACACCGTTTC+AGG	+	Chr8:52776425-52776444	MsG0880045015.01.T01:CDS	50.0%
!!	AGAGCACCGAACTACTCATC+CGG	+	Chr8:52776488-52776507	MsG0880045015.01.T01:CDS	50.0%
!!	TGAGTAGTTCGGTGCTCTTC+TGG	-	Chr8:52776486-52776505	None:intergenic	50.0%
	CGGTGCTCTTCTGGTACTTG+CGG	-	Chr8:52776477-52776496	None:intergenic	55.0%
	GACGCTGGAAAGGAAGCTTC+CGG	-	Chr8:52776510-52776529	None:intergenic	55.0%
	TTTCCAGAGCTCTGCCGTGT+CGG	+	Chr8:52776570-52776589	MsG0880045015.01.T01:CDS	55.0%
!!	ACACGGCAGAGCTCTGGAAA+CGG	-	Chr8:52776570-52776589	None:intergenic	55.0%
	AAGCCACACCGTTTCAGGCC+AGG	+	Chr8:52776430-52776449	MsG0880045015.01.T01:CDS	60.0%
	ACTGCACGCAAATCCACCGG+CGG	+	Chr8:52776337-52776356	MsG0880045015.01.T01:CDS	60.0%
	AGCTGCTCGGAAATCCGCTC+CGG	+	Chr8:52776390-52776409	MsG0880045015.01.T01:CDS	60.0%
	CACGGATACGCCTAGCAAGC+TGG	-	Chr8:52776696-52776715	None:intergenic	60.0%
	CGACGGTTCCTGGCCTGAAA+CGG	-	Chr8:52776441-52776460	None:intergenic	60.0%
	GCGGCGGAGGCTTATCTTGT+TGG	+	Chr8:52776604-52776623	MsG0880045015.01.T01:CDS	60.0%
	GTGTGGCTTCTTCACTCCGC+CGG	-	Chr8:52776419-52776438	None:intergenic	60.0%
	GTTCCTGGCCTGAAACGGTG+TGG	-	Chr8:52776436-52776455	None:intergenic	60.0%
!!	CAGCTTGCTAGGCGTATCCG+TGG	+	Chr8:52776694-52776713	MsG0880045015.01.T01:CDS	60.0%
!!	CTTCCTTGGTGCTTTGCCGC+CGG	-	Chr8:52776356-52776375	None:intergenic	60.0%
	GCGGATCTCACGGAGTGCGA+CGG	-	Chr8:52776458-52776477	None:intergenic	65.0%
	TCACGGAGTGCGACGGTTCC+TGG	-	Chr8:52776451-52776470	None:intergenic	65.0%
	TTCTTCACTCCGCCGGTCGC+CGG	-	Chr8:52776412-52776431	None:intergenic	65.0%
!	CGTGTCGGCGCTGCAAGAAG+CGG	+	Chr8:52776585-52776604	MsG0880045015.01.T01:CDS	65.0%
!!	CGCTTCTTGCAGCGCCGACA+CGG	-	Chr8:52776587-52776606	None:intergenic	65.0%
	AAATCCGCTCCGGCGACCGG+CGG	+	Chr8:52776400-52776419	MsG0880045015.01.T01:CDS	70.0%
	CGGAAATCCGCTCCGGCGAC+CGG	+	Chr8:52776397-52776416	MsG0880045015.01.T01:CDS	70.0%
	GTCGGCGCTGCAAGAAGCGG+CGG	+	Chr8:52776588-52776607	MsG0880045015.01.T01:CDS	70.0%
!	CCACCGGCGGCAAAGCACCA+AGG	+	Chr8:52776350-52776369	MsG0880045015.01.T01:CDS	70.0%
!!	CCTTGGTGCTTTGCCGCCGG+TGG	-	Chr8:52776353-52776372	None:intergenic	70.0%
	GGCGCTGCAAGAAGCGGCGG+AGG	+	Chr8:52776591-52776610	MsG0880045015.01.T01:CDS	75.0%
!	GCGCCGACACGGCAGAGCTC+TGG	-	Chr8:52776576-52776595	None:intergenic	75.0%
!	CACTCCGCCGGTCGCCGGAG+CGG	-	Chr8:52776407-52776426	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	52776319	52776729	52776319	ID=MsG0880045015.01;Name=MsG0880045015.01
Chr8	mRNA	52776319	52776729	52776319	ID=MsG0880045015.01.T01;Parent=MsG0880045015.01;Name=MsG0880045015.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
Chr8	exon	52776319	52776729	52776319	ID=MsG0880045015.01.T01:exon:22653;Parent=MsG0880045015.01.T01
Chr8	CDS	52776319	52776729	52776319	ID=MsG0880045015.01.T01:cds;Parent=MsG0880045015.01.T01

Gene Sequence

>MsG0880045015.01.T01

ATGGCACGTACAAAGCAAACTGCACGCAAATCCACCGGCGGCAAAGCACCAAGGAAGCAACTCGCAACAAAAGCTGCTCGGAAATCCGCTCCGGCGACCGGCGGAGTGAAGAAGCCACACCGTTTCAGGCCAGGAACCGTCGCACTCCGTGAGATCCGCAAGTACCAGAAGAGCACCGAACTACTCATCCGGAAGCTTCCTTTCCAGCGTCTTGTTCGTGAGATTGCTCAGGATTTCAAGACGGATCTCCGTTTCCAGAGCTCTGCCGTGTCGGCGCTGCAAGAAGCGGCGGAGGCTTATCTTGTTGGATTGTTTGAGGATACTAATCTTTGCGCTATTCATGCTAAGCGTGTTACTATCATGCCTAAGGATATCCAGCTTGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

Protein sequence

>MsG0880045015.01.T01

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*