AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023575.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023575.01.T01 MTR_8g099750 68.132 91 22 3 1 87 1 88 5.77e-32 112
MsG0480023575.01.T01 MTR_3g098810 60.870 92 29 4 1 88 1 89 6.00e-27 98.2
MsG0480023575.01.T01 MTR_3g098810 61.538 91 28 4 1 87 1 88 4.33e-26 97.8
MsG0480023575.01.T01 MTR_7g005280 48.980 98 31 5 1 88 1 89 6.29e-17 73.9
MsG0480023575.01.T01 MTR_4g081870 41.772 79 43 1 12 87 7 85 1.76e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023575.01.T01 AT5G13180 47.778 90 41 2 1 87 1 87 1.74e-17 75.5
MsG0480023575.01.T01 AT2G33480 41.593 113 47 5 1 103 1 104 2.60e-14 67.0
MsG0480023575.01.T01 AT2G33480 41.593 113 47 5 1 103 1 104 2.63e-14 67.0

Find 31 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCTATACTTTGCTTCCT+TGG 0.288379 4:+87138811 None:intergenic
TTGTTGGGTTGGCAGAACTA+TGG 0.348677 4:-87138377 MsG0480023575.01.T01:intron
AGAACAACAAATGTGGATAT+TGG 0.367929 4:-87138773 MsG0480023575.01.T01:CDS
TGTGGATATTGGAAGGCAAC+AGG 0.396475 4:-87138762 MsG0480023575.01.T01:CDS
TGTTTGCAAGTATGATCCTT+GGG 0.435717 4:-87139317 MsG0480023575.01.T01:CDS
CAAAGTATAGAAATAGTAAT+CGG 0.458697 4:-87138802 MsG0480023575.01.T01:CDS
TTCTGTGTCGGTTATCTGTA+AGG 0.460976 4:-87138335 MsG0480023575.01.T01:CDS
AACATTGACCTCAGGAATGA+TGG 0.468324 4:+87139336 None:intergenic
AACACAAGCTCTTCATCTGT+TGG 0.471066 4:+87139402 None:intergenic
GCAGAACTATGGAATGAGAT+AGG 0.489923 4:-87138366 MsG0480023575.01.T01:intron
CAAGCTCTTCATCTGTTGGT+TGG 0.493179 4:+87139406 None:intergenic
ATGGAATGAGATAGGAGAAT+GGG 0.504294 4:-87138358 MsG0480023575.01.T01:CDS
TATGATCCTTGGGATTTGCC+AGG 0.504380 4:-87139307 MsG0480023575.01.T01:intron
ATGTTTGCAAGTATGATCCT+TGG 0.507170 4:-87139318 MsG0480023575.01.T01:CDS
AGGAATGATGGAAGCAGGCA+AGG 0.516507 4:+87139348 None:intergenic
ACCTCAGGAATGATGGAAGC+AGG 0.516623 4:+87139343 None:intergenic
GCCTGCTTCCATCATTCCTG+AGG 0.534650 4:-87139344 MsG0480023575.01.T01:CDS
TACTTGCAAACATTGACCTC+AGG 0.544904 4:+87139328 None:intergenic
GGAATGATGGAAGCAGGCAA+GGG 0.549247 4:+87139349 None:intergenic
TATGGAATGAGATAGGAGAA+TGG 0.549911 4:-87138359 MsG0480023575.01.T01:CDS
CAACAAATGTGGATATTGGA+AGG 0.572876 4:-87138769 MsG0480023575.01.T01:CDS
TACTGACCTGGCAAATCCCA+AGG 0.595838 4:+87139301 None:intergenic
TAGGAGAATGGGTTCTGTGT+CGG 0.601018 4:-87138347 MsG0480023575.01.T01:CDS
GGAGTGAGTAGATTGCCTCC+TGG 0.605400 4:-87139438 MsG0480023575.01.T01:CDS
GATGAGTAGAACAACAAATG+TGG 0.615714 4:-87138780 MsG0480023575.01.T01:CDS
GTGGATATTGGAAGGCAACA+GGG 0.617430 4:-87138761 MsG0480023575.01.T01:intron
AGGAGGTTGTGATGAACAAG+AGG 0.619529 4:-87138847 MsG0480023575.01.T01:CDS
GTTATCTGTAAGGAAAGAAG+TGG 0.626924 4:-87138325 MsG0480023575.01.T01:CDS
GTTGGTTGGAAACGAAAACC+AGG 0.630000 4:+87139420 None:intergenic
GAATGATGGAAGCAGGCAAG+GGG 0.638798 4:+87139350 None:intergenic
GGTTGGAAACGAAAACCAGG+AGG 0.757632 4:+87139423 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTATTTAGTGTAAAAA+AGG + Chr4:87138752-87138771 None:intergenic 10.0%
!!! TATATAATTTAACTTTTGTT+GGG - Chr4:87139393-87139412 MsG0480023575.01.T01:CDS 10.0%
!!! TTATATAATTTAACTTTTGT+TGG - Chr4:87139392-87139411 MsG0480023575.01.T01:CDS 10.0%
!!! TTTCTAATATTTTTTTTTTG+TGG - Chr4:87138617-87138636 MsG0480023575.01.T01:intron 10.0%
!!! AAGCTGAATTTTATTAAAAA+TGG - Chr4:87138326-87138345 MsG0480023575.01.T01:CDS 15.0%
!!! TAAATCTTATTAACTAGTTT+TGG - Chr4:87138822-87138841 MsG0480023575.01.T01:CDS 15.0%
!! CAAAGTATAGAAATAGTAAT+CGG - Chr4:87138983-87139002 MsG0480023575.01.T01:intron 20.0%
!! TAGCCATTAACAAATAATAA+AGG - Chr4:87138872-87138891 MsG0480023575.01.T01:intron 20.0%
!! TATGTGAAAATAGATCATTA+TGG - Chr4:87139351-87139370 MsG0480023575.01.T01:CDS 20.0%
!! TTGTAAAAAAAATTCAACGT+TGG - Chr4:87138584-87138603 MsG0480023575.01.T01:intron 20.0%
!!! TCTCCTTTATTATTTGTTAA+TGG + Chr4:87138878-87138897 None:intergenic 20.0%
!!! TTAACATTGTTTTGTTTGAT+TGG - Chr4:87138912-87138931 MsG0480023575.01.T01:intron 20.0%
! ATTTCATCTTCAACTTGTAA+TGG - Chr4:87139058-87139077 MsG0480023575.01.T01:intron 25.0%
! GACAATGTATAATATATGCA+AGG + Chr4:87138665-87138684 None:intergenic 25.0%
!!! AAAACTTTGGTTTTCTATGA+AGG - Chr4:87139097-87139116 MsG0480023575.01.T01:intron 25.0%
!!! AAACTTTGGTTTTCTATGAA+GGG - Chr4:87139098-87139117 MsG0480023575.01.T01:intron 25.0%
!!! TAATTTAACTTTTGTTGGGT+TGG - Chr4:87139397-87139416 MsG0480023575.01.T01:CDS 25.0%
!!! TTGTTTTGTTTGATTGGTAT+AGG - Chr4:87138918-87138937 MsG0480023575.01.T01:intron 25.0%
AAATGCGTAAGTAATTACCT+GGG + Chr4:87139202-87139221 None:intergenic 30.0%
AGAACAACAAATGTGGATAT+TGG - Chr4:87139012-87139031 MsG0480023575.01.T01:intron 30.0%
ATTTCTATACTTTGCTTCCT+TGG + Chr4:87138977-87138996 None:intergenic 30.0%
TGTATAATATATGCAAGGTC+TGG + Chr4:87138660-87138679 None:intergenic 30.0%
TTGTGAAAATGAGTTAGACT+CGG + Chr4:87138534-87138553 None:intergenic 30.0%
! ACTCATTTTCACAAACTGAT+TGG - Chr4:87138540-87138559 MsG0480023575.01.T01:intron 30.0%
!! TTTGTTATTAGTTACTGACC+TGG + Chr4:87138499-87138518 None:intergenic 30.0%
!! TTTTGTTTGATTGGTATAGG+AGG - Chr4:87138921-87138940 MsG0480023575.01.T01:intron 30.0%
!!! ATTTTTTTTTTGTGGTAGCC+AGG - Chr4:87138625-87138644 MsG0480023575.01.T01:intron 30.0%
!!! TTTTTTTTTTGTGGTAGCCA+GGG - Chr4:87138626-87138645 MsG0480023575.01.T01:intron 30.0%
ATGGAATGAGATAGGAGAAT+GGG - Chr4:87139427-87139446 MsG0480023575.01.T01:CDS 35.0%
ATGTTTGCAAGTATGATCCT+TGG - Chr4:87138467-87138486 MsG0480023575.01.T01:intron 35.0%
CAAATGCGTAAGTAATTACC+TGG + Chr4:87139203-87139222 None:intergenic 35.0%
CAACAAATGTGGATATTGGA+AGG - Chr4:87139016-87139035 MsG0480023575.01.T01:intron 35.0%
CAACTTGTAATGGCATTGTA+GGG - Chr4:87139068-87139087 MsG0480023575.01.T01:intron 35.0%
GATGAGTAGAACAACAAATG+TGG - Chr4:87139005-87139024 MsG0480023575.01.T01:intron 35.0%
GTTATCTGTAAGGAAAGAAG+TGG - Chr4:87139460-87139479 MsG0480023575.01.T01:CDS 35.0%
TAGTAGTTTCCACATTGATG+AGG + Chr4:87139170-87139189 None:intergenic 35.0%
TATGAAGGGAAATCCCAAAT+GGG - Chr4:87139112-87139131 MsG0480023575.01.T01:intron 35.0%
TATGGAATGAGATAGGAGAA+TGG - Chr4:87139426-87139445 MsG0480023575.01.T01:CDS 35.0%
TCAACTTGTAATGGCATTGT+AGG - Chr4:87139067-87139086 MsG0480023575.01.T01:intron 35.0%
TGTAGGGCTAAGAAAAACTT+TGG - Chr4:87139084-87139103 MsG0480023575.01.T01:intron 35.0%
TGTGAAAATGAGTTAGACTC+GGG + Chr4:87138533-87138552 None:intergenic 35.0%
TGTTTGCAAGTATGATCCTT+GGG - Chr4:87138468-87138487 MsG0480023575.01.T01:intron 35.0%
!! AATCAGTTCTAGACCCATTT+GGG + Chr4:87139128-87139147 None:intergenic 35.0%
AACACAAGCTCTTCATCTGT+TGG + Chr4:87138386-87138405 None:intergenic 40.0%
AACATTGACCTCAGGAATGA+TGG + Chr4:87138452-87138471 None:intergenic 40.0%
AACTACTAACAACTCAACCC+AGG - Chr4:87139182-87139201 MsG0480023575.01.T01:intron 40.0%
CTATGAAGGGAAATCCCAAA+TGG - Chr4:87139111-87139130 MsG0480023575.01.T01:intron 40.0%
GCAGAACTATGGAATGAGAT+AGG - Chr4:87139419-87139438 MsG0480023575.01.T01:CDS 40.0%
TACTTGCAAACATTGACCTC+AGG + Chr4:87138460-87138479 None:intergenic 40.0%
TGAATATCGCCTCATCAATG+TGG - Chr4:87139158-87139177 MsG0480023575.01.T01:intron 40.0%
! CAAATGGGTCTAGAACTGAT+TGG - Chr4:87139127-87139146 MsG0480023575.01.T01:intron 40.0%
! TGAGCTTAGATCAGTTGGTA+GGG + Chr4:87138687-87138706 None:intergenic 40.0%
!! CAATCAGTTCTAGACCCATT+TGG + Chr4:87139129-87139148 None:intergenic 40.0%
!! TTCTGTGTCGGTTATCTGTA+AGG - Chr4:87139450-87139469 MsG0480023575.01.T01:CDS 40.0%
AGGAGGTTGTGATGAACAAG+AGG - Chr4:87138938-87138957 MsG0480023575.01.T01:intron 45.0%
CAACTGATCTAAGCTCACGA+GGG - Chr4:87138690-87138709 MsG0480023575.01.T01:intron 45.0%
CAAGCTCTTCATCTGTTGGT+TGG + Chr4:87138382-87138401 None:intergenic 45.0%
GTGAGCTTAGATCAGTTGGT+AGG + Chr4:87138688-87138707 None:intergenic 45.0%
GTGGATATTGGAAGGCAACA+GGG - Chr4:87139024-87139043 MsG0480023575.01.T01:intron 45.0%
GTTGGTTGGAAACGAAAACC+AGG + Chr4:87138368-87138387 None:intergenic 45.0%
TATGATCCTTGGGATTTGCC+AGG - Chr4:87138478-87138497 MsG0480023575.01.T01:intron 45.0%
TGATGAGGCGATATTCATGC+AGG + Chr4:87139155-87139174 None:intergenic 45.0%
TGTGGATATTGGAAGGCAAC+AGG - Chr4:87139023-87139042 MsG0480023575.01.T01:intron 45.0%
! AAGGTCTGGAATTCAAACCC+TGG + Chr4:87138646-87138665 None:intergenic 45.0%
! TAGGAGAATGGGTTCTGTGT+CGG - Chr4:87139438-87139457 MsG0480023575.01.T01:CDS 45.0%
!! TTGTTGGGTTGGCAGAACTA+TGG - Chr4:87139408-87139427 MsG0480023575.01.T01:CDS 45.0%
CCAACTGATCTAAGCTCACG+AGG - Chr4:87138689-87138708 MsG0480023575.01.T01:intron 50.0%
CCTCGTGAGCTTAGATCAGT+TGG + Chr4:87138692-87138711 None:intergenic 50.0%
GGTTGGAAACGAAAACCAGG+AGG + Chr4:87138365-87138384 None:intergenic 50.0%
TACTGACCTGGCAAATCCCA+AGG + Chr4:87138487-87138506 None:intergenic 50.0%
! ACCTCAGGAATGATGGAAGC+AGG + Chr4:87138445-87138464 None:intergenic 50.0%
! AGGAATGATGGAAGCAGGCA+AGG + Chr4:87138440-87138459 None:intergenic 50.0%
! GAATGATGGAAGCAGGCAAG+GGG + Chr4:87138438-87138457 None:intergenic 50.0%
! GGAATGATGGAAGCAGGCAA+GGG + Chr4:87138439-87138458 None:intergenic 50.0%
!!! GAGGCACTTTTTCAGCTCCA+AGG - Chr4:87138957-87138976 MsG0480023575.01.T01:intron 50.0%
GCCTGCTTCCATCATTCCTG+AGG - Chr4:87138441-87138460 MsG0480023575.01.T01:intron 55.0%
GGAGTGAGTAGATTGCCTCC+TGG - Chr4:87138347-87138366 MsG0480023575.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 87138320 87139487 87138320 ID=MsG0480023575.01;Name=MsG0480023575.01
Chr4 mRNA 87138320 87139487 87138320 ID=MsG0480023575.01.T01;Parent=MsG0480023575.01;Name=MsG0480023575.01.T01;_AED=0.26;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|116
Chr4 exon 87139308 87139487 87139308 ID=MsG0480023575.01.T01:exon:6518;Parent=MsG0480023575.01.T01
Chr4 exon 87138762 87138867 87138762 ID=MsG0480023575.01.T01:exon:6517;Parent=MsG0480023575.01.T01
Chr4 exon 87138320 87138384 87138320 ID=MsG0480023575.01.T01:exon:6516;Parent=MsG0480023575.01.T01
Chr4 CDS 87139308 87139487 87139308 ID=MsG0480023575.01.T01:cds;Parent=MsG0480023575.01.T01
Chr4 CDS 87138762 87138867 87138762 ID=MsG0480023575.01.T01:cds;Parent=MsG0480023575.01.T01
Chr4 CDS 87138320 87138384 87138320 ID=MsG0480023575.01.T01:cds;Parent=MsG0480023575.01.T01
Gene Sequence

>MsG0480023575.01.T01

ATGGATAAGCTGAATTTTATTAAAAATGGAGTGAGTAGATTGCCTCCTGGTTTTCGTTTCCAACCAACAGATGAAGAGCTTGTGTTTAATTATTTGAAATGTAAATCTTCTCATGCCCCTTGCCTGCTTCCATCATTCCTGAGGTCAATGTTTGCAAGTATGATCCTTGGGATTTGCCAGGAGGTTGTGATGAACAAGAGGCACTTTTTCAGCTCCAAGGAAGCAAAGTATAGAAATAGTAATCGGATGAGTAGAACAACAAATGTGGATATTGGAAGGCAACAGGAACTATGGAATGAGATAGGAGAATGGGTTCTGTGTCGGTTATCTGTAAGGAAAGAAGTGGTATAG

Protein sequence

>MsG0480023575.01.T01

MDKLNFIKNGVSRLPPGFRFQPTDEELVFNYLKCKSSHAPCLLPSFLRSMFASMILGICQEVVMNKRHFFSSKEAKYRNSNRMSRTTNVDIGRQQELWNEIGEWVLCRLSVRKEVV*