Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041851.01.T01 | GAU11664.1 | 98.901 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 1.40E-58 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041851.01.T01 | Q9M117 | 81.176 | 85 | 16 | 0 | 7 | 91 | 4 | 88 | 7.02E-44 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041851.01.T01 | A0A072TLU8 | 98.901 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 7.41e-59 | 186 |
| MsG0880041851.01.T01 | A0A2Z6LGG2 | 98.901 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 6.70e-59 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000139.01 | MsG0880041851.01 | 0.801411 | 9.117864e-49 | 4.351373e-46 |
| MsG0180003651.01 | MsG0880041851.01 | 0.801256 | 9.809193e-49 | 4.663129e-46 |
| MsG0180004482.01 | MsG0880041851.01 | 0.808472 | 3.045967e-50 | 1.743207e-47 |
| MsG0180005193.01 | MsG0880041851.01 | 0.816132 | 6.474150e-52 | 4.549190e-49 |
| MsG0180005424.01 | MsG0880041851.01 | 0.804158 | 2.470303e-49 | 1.264590e-46 |
| MsG0180006144.01 | MsG0880041851.01 | 0.803289 | 3.742394e-49 | 1.873397e-46 |
| MsG0780040858.01 | MsG0880041851.01 | 0.845640 | 3.580823e-59 | 6.006450e-56 |
| MsG0280011339.01 | MsG0880041851.01 | 0.806839 | 6.768751e-50 | 3.711996e-47 |
| MsG0680031488.01 | MsG0880041851.01 | 0.812885 | 3.384691e-51 | 2.177494e-48 |
| MsG0680031650.01 | MsG0880041851.01 | 0.808057 | 3.734627e-50 | 2.113960e-47 |
| MsG0480020874.01 | MsG0880041851.01 | 0.804087 | 2.556455e-49 | 1.306285e-46 |
| MsG0480021750.01 | MsG0880041851.01 | 0.805322 | 1.411762e-49 | 7.446673e-47 |
| MsG0480022707.01 | MsG0880041851.01 | 0.806989 | 6.291152e-50 | 3.463378e-47 |
| MsG0380015566.01 | MsG0880041851.01 | 0.806653 | 7.409166e-50 | 4.043376e-47 |
| MsG0380017947.01 | MsG0880041851.01 | 0.810466 | 1.136953e-50 | 6.858380e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041851.01.T01 | MTR_8g006725 | 98.901 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 1.88e-62 | 186 |
| MsG0880041851.01.T01 | MTR_1g112900 | 97.802 | 91 | 2 | 0 | 1 | 91 | 1 | 91 | 7.82e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041851.01.T01 | AT4G01710 | 81.176 | 85 | 16 | 0 | 7 | 91 | 4 | 88 | 7.16e-45 | 141 |
| MsG0880041851.01.T01 | AT5G65274 | 73.626 | 91 | 24 | 0 | 1 | 91 | 1 | 91 | 6.47e-43 | 136 |
Find 19 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGGGATGCTTTCTTCCT+TGG | 0.303924 | 8:-233665 | MsG0880041851.01.T01:CDS |
| ATTTCACCACTCACTTCATT+AGG | 0.311638 | 8:+233623 | None:intergenic |
| TGTTGTGCATAGAGCAATAA+TGG | 0.397532 | 8:-233701 | MsG0880041851.01.T01:CDS |
| GTGTTCAATTCTTGTAATGA+TGG | 0.427929 | 8:+234661 | None:intergenic |
| GATGCTTTCTTCCTTGGATC+CGG | 0.428061 | 8:-233659 | MsG0880041851.01.T01:CDS |
| GAAAATCACTCTGATTTGTA+TGG | 0.460107 | 8:-234723 | MsG0880041851.01.T01:exon |
| GCCCTTAAAACTGCACTTGA+AGG | 0.464123 | 8:-234489 | MsG0880041851.01.T01:CDS |
| ATTAGGATATCATAGTATTC+CGG | 0.466435 | 8:+233640 | None:intergenic |
| ATCACTCTGATTTGTATGGC+AGG | 0.487625 | 8:-234719 | MsG0880041851.01.T01:exon |
| TTCGGGAAAATTGAAGCTGA+AGG | 0.511039 | 8:-234807 | None:intergenic |
| ATGGCTATAAAAGATGTGGA+TGG | 0.521724 | 8:-233682 | MsG0880041851.01.T01:CDS |
| ATTTGTATGGCAGGATCAGA+AGG | 0.546400 | 8:-234710 | MsG0880041851.01.T01:exon |
| GACTAGTGATGAACGTTGCA+AGG | 0.550130 | 8:-234454 | MsG0880041851.01.T01:intron |
| ATCATAGTATTCCGGATCCA+AGG | 0.569553 | 8:+233648 | None:intergenic |
| TGTTACATACTGTTTGAGCA+AGG | 0.595063 | 8:+234619 | None:intergenic |
| GCAACGTTCATCACTAGTCA+TGG | 0.601987 | 8:+234458 | None:intergenic |
| AATAATGGCTATAAAAGATG+TGG | 0.611946 | 8:-233686 | MsG0880041851.01.T01:CDS |
| TGGCTATAAAAGATGTGGAT+GGG | 0.612168 | 8:-233681 | MsG0880041851.01.T01:CDS |
| TTCATCACTAGTCATGGCAT+AGG | 0.655247 | 8:+234464 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAAAAAAAA+AGG | + | Chr8:234206-234225 | None:intergenic | 0.0% |
| !!! | TATTTTTTTTCTTTCAATTT+TGG | - | Chr8:234687-234706 | MsG0880041851.01.T01:CDS | 10.0% |
| !! | AAATGCATAAAAATAGTAAA+AGG | + | Chr8:234014-234033 | None:intergenic | 15.0% |
| !! | AATGCATAAAAATAGTAAAA+GGG | + | Chr8:234013-234032 | None:intergenic | 15.0% |
| !! | TTATCATCTTAAATTCATTT+GGG | + | Chr8:234304-234323 | None:intergenic | 15.0% |
| !! | ATGCATAAAAATAGTAAAAG+GGG | + | Chr8:234012-234031 | None:intergenic | 20.0% |
| !! | GTTATCATCTTAAATTCATT+TGG | + | Chr8:234305-234324 | None:intergenic | 20.0% |
| !!! | GAATTTAAGATGATAACTTA+GGG | - | Chr8:234308-234327 | MsG0880041851.01.T01:intron | 20.0% |
| !!! | TGAATTTAAGATGATAACTT+AGG | - | Chr8:234307-234326 | MsG0880041851.01.T01:intron | 20.0% |
| !!! | TTTATCTTTTGCTATACAAA+TGG | - | Chr8:234500-234519 | MsG0880041851.01.T01:CDS | 20.0% |
| !!! | TTTGTGCTTTTATTTCATTT+GGG | - | Chr8:234063-234082 | MsG0880041851.01.T01:intron | 20.0% |
| !!! | TTTTGTGCTTTTATTTCATT+TGG | - | Chr8:234062-234081 | MsG0880041851.01.T01:intron | 20.0% |
| ! | AATAATGGCTATAAAAGATG+TGG | - | Chr8:234742-234761 | MsG0880041851.01.T01:five_prime_UTR | 25.0% |
| ! | ATTAGGATATCATAGTATTC+CGG | + | Chr8:234791-234810 | None:intergenic | 25.0% |
| !! | AGAAAAGCAAAACATTTTCT+CGG | - | Chr8:233647-233666 | MsG0880041851.01.T01:CDS | 25.0% |
| !! | CAATTTTAGATATAAACCAG+TGG | - | Chr8:233914-233933 | MsG0880041851.01.T01:intron | 25.0% |
| !! | TATGTTTTGTGAGACTAATT+AGG | - | Chr8:234369-234388 | MsG0880041851.01.T01:intron | 25.0% |
| !! | TTGTTTCTTCAACATCAATA+AGG | + | Chr8:234599-234618 | None:intergenic | 25.0% |
| AAAAATAGTAAAAGGGGCTA+AGG | + | Chr8:234006-234025 | None:intergenic | 30.0% | |
| GTGTTCAATTCTTGTAATGA+TGG | + | Chr8:233770-233789 | None:intergenic | 30.0% | |
| TTGAATGTGATCACATATGT+CGG | + | Chr8:234175-234194 | None:intergenic | 30.0% | |
| ! | GAAAATCACTCTGATTTGTA+TGG | - | Chr8:233705-233724 | MsG0880041851.01.T01:CDS | 30.0% |
| !!! | GCTTTAGAGTTTTGTACAAT+TGG | - | Chr8:234273-234292 | MsG0880041851.01.T01:intron | 30.0% |
| AACTTAGGGTCTGTTTGAAT+TGG | - | Chr8:234322-234341 | MsG0880041851.01.T01:intron | 35.0% | |
| ATGGCTATAAAAGATGTGGA+TGG | - | Chr8:234746-234765 | MsG0880041851.01.T01:five_prime_UTR | 35.0% | |
| TGGCTATAAAAGATGTGGAT+GGG | - | Chr8:234747-234766 | MsG0880041851.01.T01:five_prime_UTR | 35.0% | |
| TGTTACATACTGTTTGAGCA+AGG | + | Chr8:233812-233831 | None:intergenic | 35.0% | |
| TGTTGTGCATAGAGCAATAA+TGG | - | Chr8:234727-234746 | MsG0880041851.01.T01:five_prime_UTR | 35.0% | |
| ! | TTCCTTCAAGTGCAGTTTTA+AGG | + | Chr8:233944-233963 | None:intergenic | 35.0% |
| !! | AACATCAATAAGGCACTGTT+TGG | + | Chr8:234589-234608 | None:intergenic | 35.0% |
| !! | TCCTTCAAGTGCAGTTTTAA+GGG | + | Chr8:233943-233962 | None:intergenic | 35.0% |
| ACAGACACAAACACGAACAT+TGG | + | Chr8:234245-234264 | None:intergenic | 40.0% | |
| ATCATAGTATTCCGGATCCA+AGG | + | Chr8:234783-234802 | None:intergenic | 40.0% | |
| ATTGAGAGTGAGAGAGAGTA+AGG | + | Chr8:233837-233856 | None:intergenic | 40.0% | |
| ATTTGTATGGCAGGATCAGA+AGG | - | Chr8:233718-233737 | MsG0880041851.01.T01:CDS | 40.0% | |
| CTTCTAATCGAAGACGTGTT+TGG | - | Chr8:234127-234146 | MsG0880041851.01.T01:intron | 40.0% | |
| TTCATCACTAGTCATGGCAT+AGG | + | Chr8:233967-233986 | None:intergenic | 40.0% | |
| ! | ACGTCTTCGATTAGAAGCAT+TGG | + | Chr8:234124-234143 | None:intergenic | 40.0% |
| ! | ATCACTCTGATTTGTATGGC+AGG | - | Chr8:233709-233728 | MsG0880041851.01.T01:CDS | 40.0% |
| ! | CGGTGTCAAACACTAAAATG+TGG | + | Chr8:234155-234174 | None:intergenic | 40.0% |
| !! | AATTTTGGCAGTCAGCACAT+TGG | - | Chr8:234702-234721 | MsG0880041851.01.T01:CDS | 40.0% |
| GACTAGTGATGAACGTTGCA+AGG | - | Chr8:233974-233993 | MsG0880041851.01.T01:intron | 45.0% | |
| GATGCTTTCTTCCTTGGATC+CGG | - | Chr8:234769-234788 | MsG0880041851.01.T01:five_prime_UTR | 45.0% | |
| GCAACGTTCATCACTAGTCA+TGG | + | Chr8:233973-233992 | None:intergenic | 45.0% | |
| GCCCTTAAAACTGCACTTGA+AGG | - | Chr8:233939-233958 | MsG0880041851.01.T01:intron | 45.0% | |
| !!! | TAATAAAGTTTAAATTTTTA+TGG | + | Chr8:234039-234058 | None:intergenic | 5.0% |
| GGATGGGATGCTTTCTTCCT+TGG | - | Chr8:234763-234782 | MsG0880041851.01.T01:five_prime_UTR | 50.0% | |
| !!! | GCAGTTTTAAGGGCTTCCAC+TGG | + | Chr8:233933-233952 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 233633 | 234817 | 233633 | ID=MsG0880041851.01;Name=MsG0880041851.01 |
| Chr8 | mRNA | 233633 | 234817 | 233633 | ID=MsG0880041851.01.T01;Parent=MsG0880041851.01;Name=MsG0880041851.01.T01;_AED=0.46;_eAED=0.47;_QI=91|1|0.66|1|1|1|3|0|91 |
| Chr8 | exon | 234629 | 234817 | 234629 | ID=MsG0880041851.01.T01:exon:276;Parent=MsG0880041851.01.T01 |
| Chr8 | exon | 234455 | 234527 | 234455 | ID=MsG0880041851.01.T01:exon:277;Parent=MsG0880041851.01.T01 |
| Chr8 | exon | 233633 | 233737 | 233633 | ID=MsG0880041851.01.T01:exon:278;Parent=MsG0880041851.01.T01 |
| Chr8 | five_prime_UTR | 234727 | 234817 | 234727 | ID=MsG0880041851.01.T01:five_prime_utr;Parent=MsG0880041851.01.T01 |
| Chr8 | CDS | 234629 | 234726 | 234629 | ID=MsG0880041851.01.T01:cds;Parent=MsG0880041851.01.T01 |
| Chr8 | CDS | 234455 | 234527 | 234455 | ID=MsG0880041851.01.T01:cds;Parent=MsG0880041851.01.T01 |
| Chr8 | CDS | 233633 | 233737 | 233633 | ID=MsG0880041851.01.T01:cds;Parent=MsG0880041851.01.T01 |
| Gene Sequence |
| Protein sequence |
