AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042677.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042677.01.T01 MTR_8g019440 91.398 186 16 0 47 232 1 186 3.95e-116 329
MsG0880042677.01.T01 MTR_8g019060 67.123 219 71 1 1 219 44 261 9.61e-92 277
MsG0880042677.01.T01 MTR_8g019060 68.817 186 55 3 26 209 259 443 3.72e-73 229
MsG0880042677.01.T01 MTR_8g019450 50.206 243 101 3 1 226 1 240 3.82e-76 230
MsG0880042677.01.T01 MTR_8g018980 48.548 241 106 2 1 224 1 240 3.61e-71 218
MsG0880042677.01.T01 MTR_0470s0020 47.303 241 109 2 1 224 1 240 1.38e-70 216
MsG0880042677.01.T01 MTR_8g019050 47.303 241 109 2 1 224 1 240 1.38e-70 216
MsG0880042677.01.T01 MTR_0514s0010 48.133 241 107 3 1 224 3 242 5.29e-70 215
MsG0880042677.01.T01 MTR_8g019370 69.811 159 46 2 65 222 1 158 1.43e-67 206
MsG0880042677.01.T01 MTR_8g019380 46.444 239 114 5 1 226 1 238 9.59e-63 197
MsG0880042677.01.T01 MTR_8g019260 47.183 142 68 2 38 179 8 142 4.72e-37 128
MsG0880042677.01.T01 MTR_8g019110 47.183 142 68 2 38 179 8 142 4.72e-37 128
MsG0880042677.01.T01 MTR_8g019290 45.775 142 70 2 38 179 8 142 8.56e-36 125
MsG0880042677.01.T01 MTR_7g055630 46.043 139 67 3 42 177 13 146 4.72e-32 115
MsG0880042677.01.T01 MTR_0470s0010 43.571 140 78 1 38 177 10 148 9.01e-31 112
MsG0880042677.01.T01 MTR_8g019020 43.571 140 78 1 38 177 10 148 9.01e-31 112
MsG0880042677.01.T01 MTR_8g019280 48.305 118 54 2 62 179 2 112 2.81e-27 102
MsG0880042677.01.T01 MTR_1g069110 31.884 207 124 5 6 209 242 434 2.71e-22 94.7
MsG0880042677.01.T01 MTR_7g099570 34.682 173 103 4 39 209 424 588 2.65e-20 89.4
MsG0880042677.01.T01 MTR_7g087540 34.356 163 92 4 37 197 26 175 2.84e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042677.01.T01 AT3G13950 27.841 176 107 5 38 207 24 185 3.10e-14 69.3

Find 52 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACACTGCGTATTATTATT+TGG 0.053800 8:+12478227 MsG0880042677.01.T01:CDS
TTTCAACATTGCTTCGTATT+TGG 0.140918 8:+12478173 MsG0880042677.01.T01:CDS
CACACTGCGTATTATTATTT+GGG 0.201281 8:+12478228 MsG0880042677.01.T01:CDS
TTACCGAATAGCAATATTAA+TGG 0.237697 8:+12478111 MsG0880042677.01.T01:CDS
AGGAAGTGAGAATAGTATTC+AGG 0.264992 8:-12477950 None:intergenic
TGGTTACCCCAGATCCTGTC+TGG 0.315673 8:+12478131 MsG0880042677.01.T01:CDS
AGCTTTCTCCAACCTTTCTT+TGG 0.316627 8:-12477680 None:intergenic
ACCTTTCTTTGGTTCGTCAT+TGG 0.323590 8:-12477669 None:intergenic
TTATCTCCATCGTCTTTAAT+AGG 0.335574 8:-12477845 None:intergenic
GTGGAATTCGTTTGAGTCAT+AGG 0.336965 8:+12478029 MsG0880042677.01.T01:CDS
AGCATGTTCTAAAGGTTATA+AGG 0.340199 8:+12477883 MsG0880042677.01.T01:CDS
TTCTTTGGTTCGTCATTGGT+TGG 0.355017 8:-12477665 None:intergenic
TCTTTGGTTCGTCATTGGTT+GGG 0.357553 8:-12477664 None:intergenic
TTATCACCCCAGACAGGATC+TGG 0.370204 8:-12478139 None:intergenic
TAACCATTAATATTGCTATT+CGG 0.415211 8:-12478114 None:intergenic
CTTTGGTTCGTCATTGGTTG+GGG 0.416447 8:-12477663 None:intergenic
AACATATTGTGTTCCAGAAA+AGG 0.437742 8:-12477970 None:intergenic
ACATTGCTTCGTATTTGGAT+TGG 0.451577 8:+12478178 MsG0880042677.01.T01:CDS
GGAAGTGAGAATAGTATTCA+GGG 0.452495 8:-12477949 None:intergenic
CAAGCCGATGCAATCATCAT+TGG 0.452634 8:-12478298 None:intergenic
AGCAACAATAACGTTTCAAA+TGG 0.467865 8:+12477796 MsG0880042677.01.T01:CDS
TAAGGACTTGAAGCTATGTC+CGG 0.473893 8:+12477901 MsG0880042677.01.T01:CDS
TAAATCCACCCGGTGGCGTT+AGG 0.490777 8:+12477822 MsG0880042677.01.T01:CDS
AATAATACAGCATGTTCTAA+AGG 0.504058 8:+12477875 MsG0880042677.01.T01:CDS
GGTTACCCCAGATCCTGTCT+GGG 0.508011 8:+12478132 MsG0880042677.01.T01:CDS
AACAATCAAGTGGATAATCA+TGG 0.516221 8:+12477626 MsG0880042677.01.T01:CDS
TCCTGTCTGGGGTGATAATG+AGG 0.519092 8:+12478144 MsG0880042677.01.T01:CDS
TGAGTCATAGGTTTAGTATG+TGG 0.529830 8:+12478041 MsG0880042677.01.T01:CDS
CGAAAACAAGAATGAGTGGT+TGG 0.531380 8:+12477733 MsG0880042677.01.T01:CDS
TTTAATAGGCCTAACGCCAC+CGG 0.537214 8:-12477831 None:intergenic
ACACTGCGTATTATTATTTG+GGG 0.542002 8:+12478229 MsG0880042677.01.T01:CDS
ACCAATGACGAACCAAAGAA+AGG 0.542566 8:+12477668 MsG0880042677.01.T01:CDS
TATCACCCCAGACAGGATCT+GGG 0.544206 8:-12478138 None:intergenic
ATGACGAACCAAAGAAAGGT+TGG 0.557249 8:+12477672 MsG0880042677.01.T01:CDS
CACTCCAATGATGATTGCAT+CGG 0.588109 8:+12478294 MsG0880042677.01.T01:CDS
ACCTCATTATCACCCCAGAC+AGG 0.593150 8:-12478145 None:intergenic
AAGGACTTGAAGCTATGTCC+GGG 0.593407 8:+12477902 MsG0880042677.01.T01:CDS
TTAATAGGCCTAACGCCACC+GGG 0.595486 8:-12477830 None:intergenic
TGTGAGTAACAAACCCGAGA+AGG 0.604751 8:-12478209 None:intergenic
GAGTGGTTGGATCAAATGAG+AGG 0.610628 8:+12477746 MsG0880042677.01.T01:CDS
ATGGCTAAGACAGCATTACC+CGG 0.613591 8:-12477920 None:intergenic
ACAGACAATGAACAATCAAG+TGG 0.632140 8:+12477616 None:intergenic
CGATGCAATCATCATTGGAG+TGG 0.636920 8:-12478293 None:intergenic
AGTGGTTGGATCAAATGAGA+GGG 0.637995 8:+12477747 MsG0880042677.01.T01:CDS
CATACGAAAACAAGAATGAG+TGG 0.644319 8:+12477729 MsG0880042677.01.T01:CDS
CAAATGGCTATAAATCCACC+CGG 0.647825 8:+12477812 MsG0880042677.01.T01:CDS
ATCACCCCAGACAGGATCTG+GGG 0.669371 8:-12478137 None:intergenic
ATAGTATTCAGGGTATACAA+TGG 0.671783 8:-12477939 None:intergenic
GTTAGGCCTATTAAAGACGA+TGG 0.688519 8:+12477839 MsG0880042677.01.T01:CDS
ATAGGCCTAACGCCACCGGG+TGG 0.692695 8:-12477827 None:intergenic
GTTACCCCAGATCCTGTCTG+GGG 0.708353 8:+12478133 MsG0880042677.01.T01:CDS
ATGGCTATAAATCCACCCGG+TGG 0.752819 8:+12477815 MsG0880042677.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATGATTTCCTCAAAAA+AGG + Chr8:12478253-12478272 MsG0880042677.01.T01:CDS 20.0%
!!! TAACCATTAATATTGCTATT+CGG - Chr8:12478117-12478136 None:intergenic 20.0%
! AATAATACAGCATGTTCTAA+AGG + Chr8:12477875-12477894 MsG0880042677.01.T01:CDS 25.0%
! CAAAAAAGGTGAATGTAAAA+AGG + Chr8:12478267-12478286 MsG0880042677.01.T01:CDS 25.0%
! TTACCGAATAGCAATATTAA+TGG + Chr8:12478111-12478130 MsG0880042677.01.T01:CDS 25.0%
!!! TTTTACATTCACCTTTTTTG+AGG - Chr8:12478267-12478286 None:intergenic 25.0%
!!! TTTTAGAACTAGTAGTGAAT+TGG + Chr8:12477702-12477721 MsG0880042677.01.T01:CDS 25.0%
AACAATCAAGTGGATAATCA+TGG + Chr8:12477626-12477645 MsG0880042677.01.T01:CDS 30.0%
AACATATTGTGTTCCAGAAA+AGG - Chr8:12477973-12477992 None:intergenic 30.0%
ACACTGCGTATTATTATTTG+GGG + Chr8:12478229-12478248 MsG0880042677.01.T01:CDS 30.0%
AGCAACAATAACGTTTCAAA+TGG + Chr8:12477796-12477815 MsG0880042677.01.T01:CDS 30.0%
ATAGTATTCAGGGTATACAA+TGG - Chr8:12477942-12477961 None:intergenic 30.0%
CACACTGCGTATTATTATTT+GGG + Chr8:12478228-12478247 MsG0880042677.01.T01:CDS 30.0%
TCACACTGCGTATTATTATT+TGG + Chr8:12478227-12478246 MsG0880042677.01.T01:CDS 30.0%
TTATCTCCATCGTCTTTAAT+AGG - Chr8:12477848-12477867 None:intergenic 30.0%
TTTCAACATTGCTTCGTATT+TGG + Chr8:12478173-12478192 MsG0880042677.01.T01:CDS 30.0%
!! AGCATGTTCTAAAGGTTATA+AGG + Chr8:12477883-12477902 MsG0880042677.01.T01:CDS 30.0%
ACATTGCTTCGTATTTGGAT+TGG + Chr8:12478178-12478197 MsG0880042677.01.T01:CDS 35.0%
AGGAAGTGAGAATAGTATTC+AGG - Chr8:12477953-12477972 None:intergenic 35.0%
CATACGAAAACAAGAATGAG+TGG + Chr8:12477729-12477748 MsG0880042677.01.T01:CDS 35.0%
GGAAGTGAGAATAGTATTCA+GGG - Chr8:12477952-12477971 None:intergenic 35.0%
TGAGTCATAGGTTTAGTATG+TGG + Chr8:12478041-12478060 MsG0880042677.01.T01:CDS 35.0%
! ACTATTCTCACTTCCTTTTC+TGG + Chr8:12477957-12477976 MsG0880042677.01.T01:CDS 35.0%
! ATTGGATTGTTTTCCTTCTC+GGG + Chr8:12478196-12478215 MsG0880042677.01.T01:CDS 35.0%
! GATTGGATTGTTTTCCTTCT+CGG + Chr8:12478195-12478214 MsG0880042677.01.T01:CDS 35.0%
! GTTTTACTCTTGCTTATGAG+TGG + Chr8:12478010-12478029 MsG0880042677.01.T01:CDS 35.0%
!!! AGTATGTGGCTTTTTTCGAT+AGG + Chr8:12478055-12478074 MsG0880042677.01.T01:CDS 35.0%
!!! GTATGTGGCTTTTTTCGATA+GGG + Chr8:12478056-12478075 MsG0880042677.01.T01:CDS 35.0%
ACCAATGACGAACCAAAGAA+AGG + Chr8:12477668-12477687 MsG0880042677.01.T01:CDS 40.0%
AGCTTTCTCCAACCTTTCTT+TGG - Chr8:12477683-12477702 None:intergenic 40.0%
AGTGGTTGGATCAAATGAGA+GGG + Chr8:12477747-12477766 MsG0880042677.01.T01:CDS 40.0%
ATGACGAACCAAAGAAAGGT+TGG + Chr8:12477672-12477691 MsG0880042677.01.T01:CDS 40.0%
CAAATGGCTATAAATCCACC+CGG + Chr8:12477812-12477831 MsG0880042677.01.T01:CDS 40.0%
CACTCCAATGATGATTGCAT+CGG + Chr8:12478294-12478313 MsG0880042677.01.T01:CDS 40.0%
CGAAAACAAGAATGAGTGGT+TGG + Chr8:12477733-12477752 MsG0880042677.01.T01:CDS 40.0%
GTGGAATTCGTTTGAGTCAT+AGG + Chr8:12478029-12478048 MsG0880042677.01.T01:CDS 40.0%
GTTAGGCCTATTAAAGACGA+TGG + Chr8:12477839-12477858 MsG0880042677.01.T01:CDS 40.0%
TAAGGACTTGAAGCTATGTC+CGG + Chr8:12477901-12477920 MsG0880042677.01.T01:CDS 40.0%
! ACCTTTCTTTGGTTCGTCAT+TGG - Chr8:12477672-12477691 None:intergenic 40.0%
! TCTTTGGTTCGTCATTGGTT+GGG - Chr8:12477667-12477686 None:intergenic 40.0%
! TTCTTTGGTTCGTCATTGGT+TGG - Chr8:12477668-12477687 None:intergenic 40.0%
AAGGACTTGAAGCTATGTCC+GGG + Chr8:12477902-12477921 MsG0880042677.01.T01:CDS 45.0%
ATGGCTAAGACAGCATTACC+CGG - Chr8:12477923-12477942 None:intergenic 45.0%
CAAGCCGATGCAATCATCAT+TGG - Chr8:12478301-12478320 None:intergenic 45.0%
CGATGCAATCATCATTGGAG+TGG - Chr8:12478296-12478315 None:intergenic 45.0%
GAGTGGTTGGATCAAATGAG+AGG + Chr8:12477746-12477765 MsG0880042677.01.T01:CDS 45.0%
TGTGAGTAACAAACCCGAGA+AGG - Chr8:12478212-12478231 None:intergenic 45.0%
TTTAATAGGCCTAACGCCAC+CGG - Chr8:12477834-12477853 None:intergenic 45.0%
! CTTTGGTTCGTCATTGGTTG+GGG - Chr8:12477666-12477685 None:intergenic 45.0%
ACCTCATTATCACCCCAGAC+AGG - Chr8:12478148-12478167 None:intergenic 50.0%
ATGGCTATAAATCCACCCGG+TGG + Chr8:12477815-12477834 MsG0880042677.01.T01:CDS 50.0%
TATCACCCCAGACAGGATCT+GGG - Chr8:12478141-12478160 None:intergenic 50.0%
TTAATAGGCCTAACGCCACC+GGG - Chr8:12477833-12477852 None:intergenic 50.0%
TTATCACCCCAGACAGGATC+TGG - Chr8:12478142-12478161 None:intergenic 50.0%
! TCCTGTCTGGGGTGATAATG+AGG + Chr8:12478144-12478163 MsG0880042677.01.T01:CDS 50.0%
ATCACCCCAGACAGGATCTG+GGG - Chr8:12478140-12478159 None:intergenic 55.0%
GGTTACCCCAGATCCTGTCT+GGG + Chr8:12478132-12478151 MsG0880042677.01.T01:CDS 55.0%
GTTACCCCAGATCCTGTCTG+GGG + Chr8:12478133-12478152 MsG0880042677.01.T01:CDS 55.0%
TAAATCCACCCGGTGGCGTT+AGG + Chr8:12477822-12477841 MsG0880042677.01.T01:CDS 55.0%
TGGTTACCCCAGATCCTGTC+TGG + Chr8:12478131-12478150 MsG0880042677.01.T01:CDS 55.0%
ATAGGCCTAACGCCACCGGG+TGG - Chr8:12477830-12477849 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 12477623 12478321 12477623 ID=MsG0880042677.01;Name=MsG0880042677.01
Chr8 mRNA 12477623 12478321 12477623 ID=MsG0880042677.01.T01;Parent=MsG0880042677.01;Name=MsG0880042677.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|232
Chr8 exon 12477623 12478321 12477623 ID=MsG0880042677.01.T01:exon:242;Parent=MsG0880042677.01.T01
Chr8 CDS 12477623 12478321 12477623 ID=MsG0880042677.01.T01:cds;Parent=MsG0880042677.01.T01
Gene Sequence

>MsG0880042677.01.T01

ATGAACAATCAAGTGGATAATCATGGTCGTTCAACAAAAACCCCAACCAATGACGAACCAAAGAAAGGTTGGAGAAAGCTTTTAGAACTAGTAGTGAATTGGTTGTCATACGAAAACAAGAATGAGTGGTTGGATCAAATGAGAGGGAACATGAGTATAGTAGCAATATTCATAGCAACAATAACGTTTCAAATGGCTATAAATCCACCCGGTGGCGTTAGGCCTATTAAAGACGATGGAGATAAAGACGCAAATAATACAGCATGTTCTAAAGGTTATAAGGACTTGAAGCTATGTCCGGGTAATGCTGTCTTAGCCATTGTATACCCTGAATACTATTCTCACTTCCTTTTCTGGAACACAATATGTTTCATTGCATCGCTTAGTGTTTTACTCTTGCTTATGAGTGGAATTCGTTTGAGTCATAGGTTTAGTATGTGGCTTTTTTCGATAGGGATGTGTTTCACTCTCACAACTCTTCTTGTTACTTACCGAATAGCAATATTAATGGTTACCCCAGATCCTGTCTGGGGTGATAATGAGGTTTTACTTTCAACATTGCTTCGTATTTGGATTGGATTGTTTTCCTTCTCGGGTTTGTTACTCACACTGCGTATTATTATTTGGGGAATTAATGATTTCCTCAAAAAAGGTGAATGTAAAAAGGCAACCACTCCAATGATGATTGCATCGGCTTGA

Protein sequence

>MsG0880042677.01.T01

MNNQVDNHGRSTKTPTNDEPKKGWRKLLELVVNWLSYENKNEWLDQMRGNMSIVAIFIATITFQMAINPPGGVRPIKDDGDKDANNTACSKGYKDLKLCPGNAVLAIVYPEYYSHFLFWNTICFIASLSVLLLLMSGIRLSHRFSMWLFSIGMCFTLTTLLVTYRIAILMVTPDPVWGDNEVLLSTLLRIWIGLFSFSGLLLTLRIIIWGINDFLKKGECKKATTPMMIASA*