Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042733.01.T01 | XP_003627288.2 | 83.568 | 213 | 33 | 1 | 1 | 213 | 1 | 211 | 1.04E-117 | 343 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042733.01.T01 | O04012 | 51.887 | 212 | 96 | 3 | 3 | 212 | 1 | 208 | 3.71E-63 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042733.01.T01 | G7L6W0 | 83.568 | 213 | 33 | 1 | 1 | 213 | 1 | 211 | 4.98e-118 | 343 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880041858.01 | MsG0880042733.01 | 0.828432 | 9.018934e-55 | 8.963275e-52 |
| MsG0880042733.01 | MsG0880045179.01 | 0.809063 | 2.276850e-50 | 1.323492e-47 |
| MsG0480024022.01 | MsG0880042733.01 | 0.819513 | 1.116391e-52 | 8.609171e-50 |
| MsG0280006966.01 | MsG0880042733.01 | 0.804756 | 1.854379e-49 | 9.640393e-47 |
| MsG0280006967.01 | MsG0880042733.01 | 0.842704 | 2.197584e-58 | 3.359700e-55 |
| MsG0280008392.01 | MsG0880042733.01 | 0.812916 | 3.332919e-51 | 2.145879e-48 |
| MsG0280008395.01 | MsG0880042733.01 | 0.803647 | 3.154899e-49 | 1.593935e-46 |
| MsG0680035683.01 | MsG0880042733.01 | 0.805335 | 1.402590e-49 | 7.400838e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042733.01.T01 | MTR_8g020760 | 83.568 | 213 | 33 | 1 | 1 | 213 | 1 | 211 | 1.26e-121 | 343 |
| MsG0880042733.01.T01 | MTR_4g010340 | 75.355 | 211 | 46 | 2 | 3 | 213 | 1 | 205 | 6.80e-109 | 311 |
| MsG0880042733.01.T01 | MTR_8g020763 | 80.220 | 182 | 35 | 1 | 33 | 213 | 9 | 190 | 3.86e-101 | 291 |
| MsG0880042733.01.T01 | MTR_8g020630 | 60.563 | 213 | 80 | 2 | 1 | 213 | 1 | 209 | 4.70e-89 | 261 |
| MsG0880042733.01.T01 | MTR_2g019780 | 63.426 | 216 | 72 | 4 | 1 | 213 | 1 | 212 | 4.58e-86 | 253 |
| MsG0880042733.01.T01 | MTR_8g020650 | 58.216 | 213 | 85 | 1 | 1 | 213 | 1 | 209 | 9.01e-86 | 253 |
| MsG0880042733.01.T01 | MTR_2g044040 | 59.155 | 213 | 83 | 2 | 1 | 213 | 1 | 209 | 2.36e-85 | 251 |
| MsG0880042733.01.T01 | MTR_2g045100 | 61.033 | 213 | 79 | 2 | 1 | 213 | 1 | 209 | 2.08e-84 | 249 |
| MsG0880042733.01.T01 | MTR_3g073170 | 63.551 | 214 | 73 | 3 | 1 | 213 | 1 | 210 | 2.27e-84 | 249 |
| MsG0880042733.01.T01 | MTR_8g020770 | 59.070 | 215 | 83 | 4 | 1 | 213 | 1 | 212 | 1.24e-83 | 247 |
| MsG0880042733.01.T01 | MTR_2g036120 | 59.434 | 212 | 82 | 2 | 1 | 212 | 1 | 208 | 1.19e-82 | 244 |
| MsG0880042733.01.T01 | MTR_8g020670 | 57.971 | 207 | 84 | 1 | 7 | 213 | 6 | 209 | 1.95e-81 | 241 |
| MsG0880042733.01.T01 | MTR_0284s0010 | 58.216 | 213 | 85 | 2 | 1 | 213 | 1 | 209 | 9.73e-81 | 240 |
| MsG0880042733.01.T01 | MTR_8g020750 | 59.070 | 215 | 83 | 4 | 1 | 213 | 1 | 212 | 5.99e-79 | 235 |
| MsG0880042733.01.T01 | MTR_7g011340 | 61.702 | 188 | 72 | 0 | 26 | 213 | 22 | 209 | 7.13e-79 | 235 |
| MsG0880042733.01.T01 | MTR_3g019710 | 57.746 | 213 | 86 | 2 | 1 | 213 | 1 | 209 | 3.22e-78 | 233 |
| MsG0880042733.01.T01 | MTR_3g019680 | 55.238 | 210 | 90 | 1 | 4 | 213 | 3 | 208 | 4.92e-74 | 223 |
| MsG0880042733.01.T01 | MTR_3g019650 | 55.072 | 207 | 89 | 1 | 7 | 213 | 6 | 208 | 8.32e-74 | 222 |
| MsG0880042733.01.T01 | MTR_5g031460 | 53.991 | 213 | 94 | 2 | 1 | 213 | 1 | 209 | 2.35e-73 | 221 |
| MsG0880042733.01.T01 | MTR_8g020590 | 48.804 | 209 | 101 | 3 | 7 | 213 | 4 | 208 | 1.95e-65 | 201 |
| MsG0880042733.01.T01 | MTR_2g041570 | 47.907 | 215 | 105 | 3 | 1 | 213 | 1 | 210 | 1.02e-63 | 197 |
| MsG0880042733.01.T01 | MTR_2g041580 | 47.442 | 215 | 106 | 3 | 1 | 213 | 1 | 210 | 6.33e-63 | 194 |
| MsG0880042733.01.T01 | MTR_2g041550 | 47.442 | 215 | 106 | 3 | 1 | 213 | 1 | 210 | 1.78e-61 | 191 |
| MsG0880042733.01.T01 | MTR_8g020610 | 49.282 | 209 | 102 | 3 | 7 | 213 | 1 | 207 | 4.60e-61 | 190 |
| MsG0880042733.01.T01 | MTR_3g019810 | 48.544 | 206 | 96 | 3 | 1 | 206 | 107 | 302 | 1.26e-56 | 182 |
| MsG0880042733.01.T01 | MTR_4g027415 | 61.983 | 121 | 46 | 0 | 26 | 146 | 22 | 142 | 2.12e-45 | 147 |
| MsG0880042733.01.T01 | MTR_2g072560 | 56.198 | 121 | 45 | 2 | 1 | 119 | 1 | 115 | 4.17e-36 | 124 |
| MsG0880042733.01.T01 | MTR_1g079490 | 36.406 | 217 | 130 | 3 | 1 | 209 | 1 | 217 | 6.10e-35 | 123 |
| MsG0880042733.01.T01 | MTR_3g019610 | 54.955 | 111 | 42 | 2 | 1 | 109 | 1 | 105 | 6.11e-32 | 112 |
| MsG0880042733.01.T01 | MTR_2g031270 | 36.598 | 194 | 114 | 4 | 22 | 209 | 23 | 213 | 1.57e-31 | 114 |
| MsG0880042733.01.T01 | MTR_6g005330 | 36.111 | 216 | 130 | 4 | 1 | 209 | 1 | 215 | 5.79e-31 | 113 |
| MsG0880042733.01.T01 | MTR_6g005360 | 35.648 | 216 | 131 | 4 | 1 | 209 | 1 | 215 | 2.42e-30 | 111 |
| MsG0880042733.01.T01 | MTR_6g005310 | 36.792 | 212 | 124 | 5 | 7 | 209 | 5 | 215 | 1.03e-29 | 110 |
| MsG0880042733.01.T01 | MTR_4g017050 | 35.945 | 217 | 128 | 5 | 1 | 208 | 1 | 215 | 2.73e-29 | 108 |
| MsG0880042733.01.T01 | MTR_1g112200 | 35.938 | 192 | 110 | 7 | 26 | 212 | 27 | 210 | 2.88e-29 | 108 |
| MsG0880042733.01.T01 | MTR_5g046410 | 35.945 | 217 | 128 | 5 | 1 | 208 | 1 | 215 | 2.98e-29 | 108 |
| MsG0880042733.01.T01 | MTR_5g046430 | 35.945 | 217 | 128 | 5 | 1 | 208 | 1 | 215 | 2.98e-29 | 108 |
| MsG0880042733.01.T01 | MTR_4g017030 | 35.945 | 217 | 128 | 5 | 1 | 208 | 1 | 215 | 2.98e-29 | 108 |
| MsG0880042733.01.T01 | MTR_6g005340 | 36.321 | 212 | 125 | 5 | 7 | 209 | 5 | 215 | 5.28e-29 | 108 |
| MsG0880042733.01.T01 | MTR_1g085500 | 35.514 | 214 | 122 | 6 | 7 | 210 | 8 | 215 | 8.14e-29 | 107 |
| MsG0880042733.01.T01 | MTR_1g085140 | 37.696 | 191 | 111 | 4 | 26 | 210 | 28 | 216 | 1.45e-28 | 107 |
| MsG0880042733.01.T01 | MTR_1g023770 | 35.417 | 192 | 112 | 4 | 24 | 209 | 24 | 209 | 2.07e-28 | 106 |
| MsG0880042733.01.T01 | MTR_2g030855 | 37.634 | 186 | 106 | 4 | 23 | 204 | 23 | 202 | 2.11e-28 | 106 |
| MsG0880042733.01.T01 | MTR_7g111240 | 44.595 | 148 | 79 | 3 | 25 | 169 | 23 | 170 | 2.46e-28 | 106 |
| MsG0880042733.01.T01 | MTR_1g060120 | 31.163 | 215 | 139 | 3 | 4 | 209 | 7 | 221 | 3.13e-28 | 106 |
| MsG0880042733.01.T01 | MTR_7g111240 | 45.270 | 148 | 75 | 4 | 25 | 169 | 23 | 167 | 5.09e-28 | 105 |
| MsG0880042733.01.T01 | MTR_4g017040 | 35.023 | 217 | 130 | 5 | 1 | 208 | 1 | 215 | 5.74e-28 | 105 |
| MsG0880042733.01.T01 | MTR_8g007300 | 38.150 | 173 | 95 | 6 | 8 | 172 | 11 | 179 | 7.39e-28 | 105 |
| MsG0880042733.01.T01 | MTR_2g019250 | 35.023 | 217 | 130 | 5 | 1 | 208 | 1 | 215 | 1.83e-27 | 104 |
| MsG0880042733.01.T01 | MTR_6g005380 | 40.476 | 168 | 93 | 3 | 11 | 172 | 11 | 177 | 2.30e-27 | 103 |
| MsG0880042733.01.T01 | MTR_2g030895 | 35.465 | 172 | 104 | 4 | 45 | 209 | 7 | 178 | 4.66e-25 | 97.1 |
| MsG0880042733.01.T01 | MTR_6g005350 | 33.019 | 212 | 133 | 5 | 6 | 209 | 4 | 214 | 1.03e-23 | 94.4 |
| MsG0880042733.01.T01 | MTR_2g030865 | 35.465 | 172 | 104 | 4 | 45 | 209 | 7 | 178 | 1.87e-23 | 92.8 |
| MsG0880042733.01.T01 | MTR_5g036570 | 31.414 | 191 | 126 | 4 | 26 | 211 | 31 | 221 | 2.47e-23 | 93.6 |
| MsG0880042733.01.T01 | MTR_3g096330 | 33.146 | 178 | 102 | 3 | 41 | 203 | 598 | 773 | 2.77e-23 | 97.4 |
| MsG0880042733.01.T01 | MTR_2g031300 | 44.340 | 106 | 55 | 2 | 45 | 146 | 7 | 112 | 3.61e-20 | 83.6 |
| MsG0880042733.01.T01 | MTR_2g086620 | 31.373 | 204 | 129 | 5 | 15 | 209 | 3 | 204 | 1.98e-18 | 80.1 |
| MsG0880042733.01.T01 | MTR_2g086630 | 31.373 | 204 | 129 | 5 | 15 | 209 | 3 | 204 | 2.79e-18 | 79.7 |
| MsG0880042733.01.T01 | MTR_2g086640 | 29.412 | 204 | 135 | 5 | 14 | 209 | 1 | 203 | 3.13e-18 | 79.7 |
| MsG0880042733.01.T01 | MTR_2g096110 | 36.913 | 149 | 72 | 5 | 52 | 198 | 10 | 138 | 4.07e-18 | 77.8 |
| MsG0880042733.01.T01 | MTR_7g029500 | 71.698 | 53 | 15 | 0 | 154 | 206 | 38 | 90 | 5.96e-17 | 73.2 |
| MsG0880042733.01.T01 | MTR_4g052770 | 34.146 | 164 | 103 | 3 | 51 | 209 | 42 | 205 | 2.61e-16 | 74.7 |
| MsG0880042733.01.T01 | MTR_4g052780 | 34.146 | 164 | 103 | 3 | 51 | 209 | 42 | 205 | 6.67e-16 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042733.01.T01 | AT1G72610 | 48.958 | 192 | 96 | 2 | 24 | 213 | 17 | 208 | 1.10e-60 | 189 |
| MsG0880042733.01.T01 | AT5G20630 | 45.327 | 214 | 111 | 3 | 1 | 212 | 1 | 210 | 2.44e-56 | 178 |
| MsG0880042733.01.T01 | AT5G38960 | 42.000 | 200 | 99 | 5 | 24 | 210 | 28 | 223 | 1.88e-37 | 130 |
| MsG0880042733.01.T01 | AT3G05950 | 39.614 | 207 | 117 | 4 | 11 | 209 | 15 | 221 | 9.08e-34 | 120 |
| MsG0880042733.01.T01 | AT3G04200 | 36.574 | 216 | 127 | 5 | 3 | 210 | 10 | 223 | 2.23e-32 | 117 |
| MsG0880042733.01.T01 | AT5G39190 | 36.232 | 207 | 124 | 4 | 12 | 210 | 14 | 220 | 2.42e-32 | 117 |
| MsG0880042733.01.T01 | AT5G39160 | 35.437 | 206 | 123 | 4 | 12 | 210 | 14 | 216 | 3.02e-32 | 116 |
| MsG0880042733.01.T01 | AT5G39160 | 35.749 | 207 | 125 | 4 | 12 | 210 | 14 | 220 | 5.88e-32 | 116 |
| MsG0880042733.01.T01 | AT5G39130 | 35.749 | 207 | 125 | 4 | 12 | 210 | 14 | 220 | 1.66e-31 | 115 |
| MsG0880042733.01.T01 | AT5G38940 | 38.144 | 194 | 112 | 4 | 24 | 209 | 28 | 221 | 7.58e-31 | 113 |
| MsG0880042733.01.T01 | AT5G38940 | 38.144 | 194 | 112 | 4 | 24 | 209 | 52 | 245 | 1.09e-30 | 113 |
| MsG0880042733.01.T01 | AT5G26700 | 36.181 | 199 | 118 | 4 | 17 | 210 | 16 | 210 | 1.50e-30 | 112 |
| MsG0880042733.01.T01 | AT3G05930 | 35.096 | 208 | 130 | 3 | 7 | 209 | 8 | 215 | 1.92e-30 | 112 |
| MsG0880042733.01.T01 | AT4G14630 | 37.209 | 215 | 118 | 5 | 8 | 209 | 12 | 222 | 3.71e-30 | 111 |
| MsG0880042733.01.T01 | AT5G39110 | 35.211 | 213 | 129 | 4 | 6 | 209 | 8 | 220 | 1.08e-29 | 110 |
| MsG0880042733.01.T01 | AT1G09560 | 41.615 | 161 | 92 | 2 | 26 | 184 | 28 | 188 | 1.50e-29 | 109 |
| MsG0880042733.01.T01 | AT1G02335 | 35.263 | 190 | 119 | 3 | 25 | 210 | 27 | 216 | 1.86e-29 | 109 |
| MsG0880042733.01.T01 | AT5G38910 | 35.349 | 215 | 123 | 5 | 7 | 209 | 9 | 219 | 2.96e-29 | 108 |
| MsG0880042733.01.T01 | AT5G61750 | 42.647 | 136 | 72 | 3 | 41 | 172 | 40 | 173 | 3.58e-29 | 108 |
| MsG0880042733.01.T01 | AT3G04150 | 32.536 | 209 | 133 | 4 | 13 | 213 | 17 | 225 | 4.86e-29 | 108 |
| MsG0880042733.01.T01 | AT5G38930 | 37.113 | 194 | 114 | 4 | 24 | 209 | 28 | 221 | 5.39e-29 | 108 |
| MsG0880042733.01.T01 | AT3G04190 | 35.204 | 196 | 119 | 4 | 15 | 202 | 19 | 214 | 1.17e-28 | 107 |
| MsG0880042733.01.T01 | AT1G18980 | 37.037 | 189 | 113 | 4 | 26 | 210 | 33 | 219 | 2.11e-28 | 106 |
| MsG0880042733.01.T01 | AT3G62020 | 34.742 | 213 | 124 | 6 | 9 | 210 | 8 | 216 | 2.62e-28 | 106 |
| MsG0880042733.01.T01 | AT3G04150 | 34.659 | 176 | 108 | 3 | 45 | 213 | 63 | 238 | 3.01e-28 | 107 |
| MsG0880042733.01.T01 | AT3G04180 | 34.975 | 203 | 124 | 4 | 15 | 209 | 19 | 221 | 4.57e-28 | 105 |
| MsG0880042733.01.T01 | AT5G39180 | 34.722 | 216 | 130 | 5 | 4 | 209 | 5 | 219 | 6.54e-28 | 105 |
| MsG0880042733.01.T01 | AT3G04170 | 35.849 | 212 | 115 | 8 | 11 | 209 | 15 | 218 | 1.42e-27 | 104 |
| MsG0880042733.01.T01 | AT5G39120 | 34.722 | 216 | 130 | 5 | 4 | 209 | 5 | 219 | 1.70e-27 | 104 |
| MsG0880042733.01.T01 | AT5G39150 | 34.722 | 216 | 130 | 5 | 4 | 209 | 5 | 219 | 1.87e-27 | 104 |
| MsG0880042733.01.T01 | AT3G10080 | 31.156 | 199 | 131 | 2 | 17 | 209 | 21 | 219 | 5.74e-26 | 100 |
| MsG0880042733.01.T01 | AT3G10080 | 33.503 | 197 | 111 | 5 | 26 | 209 | 101 | 290 | 1.20e-25 | 101 |
| MsG0880042733.01.T01 | AT1G18970 | 31.925 | 213 | 139 | 4 | 2 | 210 | 9 | 219 | 1.95e-25 | 99.0 |
| MsG0880042733.01.T01 | AT5G39190 | 32.195 | 205 | 113 | 3 | 12 | 210 | 14 | 198 | 2.27e-25 | 98.6 |
| MsG0880042733.01.T01 | AT3G62020 | 35.429 | 175 | 108 | 4 | 41 | 210 | 13 | 187 | 3.51e-25 | 97.8 |
| MsG0880042733.01.T01 | AT5G39160 | 31.707 | 205 | 114 | 3 | 12 | 210 | 14 | 198 | 5.61e-25 | 97.4 |
| MsG0880042733.01.T01 | AT1G10460 | 37.107 | 159 | 93 | 5 | 26 | 177 | 23 | 181 | 3.37e-24 | 95.9 |
| MsG0880042733.01.T01 | AT1G74820 | 42.857 | 133 | 72 | 3 | 27 | 157 | 41 | 171 | 1.16e-23 | 94.7 |
| MsG0880042733.01.T01 | AT5G38910 | 29.717 | 212 | 102 | 5 | 7 | 209 | 9 | 182 | 1.98e-15 | 71.6 |
Find 36 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACTTGATTATCTATTATT+TGG | 0.155412 | 8:+13347513 | MsG0880042733.01.T01:CDS |
| ACCAGTTTACCTGGTCTTAA+TGG | 0.209440 | 8:+13347225 | MsG0880042733.01.T01:CDS |
| ACAAAATGCAAAAGCCCTTC+TGG | 0.264438 | 8:-13347423 | None:intergenic |
| TCTGCTGGAAGAGGAGATTT+TGG | 0.267961 | 8:+13347258 | MsG0880042733.01.T01:CDS |
| GAATTAGTTATCGTGGTTAA+AGG | 0.280928 | 8:+13347324 | MsG0880042733.01.T01:CDS |
| GTGGTTAATCTAACATTAAA+AGG | 0.285906 | 8:-13347192 | None:intergenic |
| GTTGGAAATGCTATTGCTTT+TGG | 0.309862 | 8:+13347459 | MsG0880042733.01.T01:CDS |
| GACAGAAGAAGTTGCAGTTT+GGG | 0.342150 | 8:-13347043 | None:intergenic |
| AAAGGGTCTATTGTGTTTCC+AGG | 0.347883 | 8:-13347174 | None:intergenic |
| TGACAGAAGAAGTTGCAGTT+TGG | 0.368327 | 8:-13347044 | None:intergenic |
| TCCATTAAGACCAGGTAAAC+TGG | 0.370975 | 8:-13347226 | None:intergenic |
| ATAAAGCTTAAGAATCAGTT+TGG | 0.389749 | 8:+13347594 | MsG0880042733.01.T01:CDS |
| GGATAGCCTGAAGGAGTGTT+TGG | 0.395462 | 8:-13347096 | None:intergenic |
| GTGTTCTCTGGCTTTGTACC+TGG | 0.400296 | 8:+13347156 | MsG0880042733.01.T01:CDS |
| CAGCTTCCAAACACTCCTTC+AGG | 0.418699 | 8:+13347090 | MsG0880042733.01.T01:CDS |
| TTTGTAGTTAACTCTGGTGT+TGG | 0.425062 | 8:+13347441 | MsG0880042733.01.T01:CDS |
| GCATTTGTCACCAGTTTACC+TGG | 0.439631 | 8:+13347216 | MsG0880042733.01.T01:CDS |
| AAGAATCAGTTTGGTGGTAG+TGG | 0.444437 | 8:+13347603 | MsG0880042733.01.T01:CDS |
| TAATAAATCCAGCAGTCAAT+TGG | 0.460927 | 8:-13347347 | None:intergenic |
| TTACCTGGTCTTAATGGACT+TGG | 0.465576 | 8:+13347231 | MsG0880042733.01.T01:CDS |
| TAGTAGCTCAAACCCTAAAA+CGG | 0.473073 | 8:+13347488 | MsG0880042733.01.T01:CDS |
| AATGGACTTGGTATATCTGC+TGG | 0.482284 | 8:+13347243 | MsG0880042733.01.T01:CDS |
| ATAACTAATTCAGTTGCATC+AGG | 0.492749 | 8:-13347312 | None:intergenic |
| GTTAAAGGCCAATTGACTGC+TGG | 0.522198 | 8:+13347339 | MsG0880042733.01.T01:CDS |
| ACACTTGTTTCTGACTTGCA+TGG | 0.533166 | 8:-13347117 | None:intergenic |
| CTTGGTATATCTGCTGGAAG+AGG | 0.540629 | 8:+13347249 | MsG0880042733.01.T01:CDS |
| TGGTTAATCTAACATTAAAA+GGG | 0.555953 | 8:-13347191 | None:intergenic |
| TATTTGAAAACTGTTAAACC+TGG | 0.562860 | 8:+13347384 | MsG0880042733.01.T01:CDS |
| AAGCTTAAGAATCAGTTTGG+TGG | 0.578020 | 8:+13347597 | MsG0880042733.01.T01:CDS |
| TGCAACTGAATTAGTTATCG+TGG | 0.584807 | 8:+13347317 | MsG0880042733.01.T01:CDS |
| AGAAGAAGTTGCAGTTTGGG+AGG | 0.599718 | 8:-13347040 | None:intergenic |
| GCATCAGGATGAGTGAGCAT+TGG | 0.603045 | 8:-13347297 | None:intergenic |
| GACTTGCATGGATAGCCTGA+AGG | 0.608627 | 8:-13347105 | None:intergenic |
| TTCTGTCAATGATTTCTGTG+TGG | 0.614592 | 8:+13347059 | MsG0880042733.01.T01:CDS |
| ATACCAAGTCCATTAAGACC+AGG | 0.671415 | 8:-13347234 | None:intergenic |
| TAAACTGGTGACAAATGCAG+TGG | 0.677227 | 8:-13347211 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTTGATTATCTATTATT+TGG | + | Chr8:13347513-13347532 | MsG0880042733.01.T01:CDS | 15.0% |
| !! | TGGTTAATCTAACATTAAAA+GGG | - | Chr8:13347194-13347213 | None:intergenic | 20.0% |
| !!! | GTTTTCAAATAAACTTTTGT+TGG | - | Chr8:13347375-13347394 | None:intergenic | 20.0% |
| ! | ATAAAGCTTAAGAATCAGTT+TGG | + | Chr8:13347594-13347613 | MsG0880042733.01.T01:CDS | 25.0% |
| ! | GTGGTTAATCTAACATTAAA+AGG | - | Chr8:13347195-13347214 | None:intergenic | 25.0% |
| ! | TATTTGAAAACTGTTAAACC+TGG | + | Chr8:13347384-13347403 | MsG0880042733.01.T01:CDS | 25.0% |
| !! | AATCAAGTATTTCCGTTTTA+GGG | - | Chr8:13347503-13347522 | None:intergenic | 25.0% |
| !! | ATATTGTAGTTTTTCCAGAA+GGG | + | Chr8:13347409-13347428 | MsG0880042733.01.T01:CDS | 25.0% |
| !! | TAATCAAGTATTTCCGTTTT+AGG | - | Chr8:13347504-13347523 | None:intergenic | 25.0% |
| ATAACTAATTCAGTTGCATC+AGG | - | Chr8:13347315-13347334 | None:intergenic | 30.0% | |
| GAATTAGTTATCGTGGTTAA+AGG | + | Chr8:13347324-13347343 | MsG0880042733.01.T01:CDS | 30.0% | |
| TAATAAATCCAGCAGTCAAT+TGG | - | Chr8:13347350-13347369 | None:intergenic | 30.0% | |
| ! | GATATTGTAGTTTTTCCAGA+AGG | + | Chr8:13347408-13347427 | MsG0880042733.01.T01:CDS | 30.0% |
| ! | TTGCATTTTGTAGTTAACTC+TGG | + | Chr8:13347435-13347454 | MsG0880042733.01.T01:CDS | 30.0% |
| !! | AGAGGAGATTTTGGTATAAA+TGG | + | Chr8:13347267-13347286 | MsG0880042733.01.T01:CDS | 30.0% |
| AAGCTTAAGAATCAGTTTGG+TGG | + | Chr8:13347597-13347616 | MsG0880042733.01.T01:CDS | 35.0% | |
| GGAAAAACTACAATATCACC+AGG | - | Chr8:13347405-13347424 | None:intergenic | 35.0% | |
| TAGTAGCTCAAACCCTAAAA+CGG | + | Chr8:13347488-13347507 | MsG0880042733.01.T01:CDS | 35.0% | |
| TGCAACTGAATTAGTTATCG+TGG | + | Chr8:13347317-13347336 | MsG0880042733.01.T01:CDS | 35.0% | |
| TTCTGTCAATGATTTCTGTG+TGG | + | Chr8:13347059-13347078 | MsG0880042733.01.T01:CDS | 35.0% | |
| TTTGTAGTTAACTCTGGTGT+TGG | + | Chr8:13347441-13347460 | MsG0880042733.01.T01:CDS | 35.0% | |
| ! | GTTGGAAATGCTATTGCTTT+TGG | + | Chr8:13347459-13347478 | MsG0880042733.01.T01:CDS | 35.0% |
| AAAGGGTCTATTGTGTTTCC+AGG | - | Chr8:13347177-13347196 | None:intergenic | 40.0% | |
| ACAAAATGCAAAAGCCCTTC+TGG | - | Chr8:13347426-13347445 | None:intergenic | 40.0% | |
| ATACCAAGTCCATTAAGACC+AGG | - | Chr8:13347237-13347256 | None:intergenic | 40.0% | |
| GACAGAAGAAGTTGCAGTTT+GGG | - | Chr8:13347046-13347065 | None:intergenic | 40.0% | |
| TAAACTGGTGACAAATGCAG+TGG | - | Chr8:13347214-13347233 | None:intergenic | 40.0% | |
| TCCATTAAGACCAGGTAAAC+TGG | - | Chr8:13347229-13347248 | None:intergenic | 40.0% | |
| TGACAGAAGAAGTTGCAGTT+TGG | - | Chr8:13347047-13347066 | None:intergenic | 40.0% | |
| ! | AAGAATCAGTTTGGTGGTAG+TGG | + | Chr8:13347603-13347622 | MsG0880042733.01.T01:CDS | 40.0% |
| ! | AATGGACTTGGTATATCTGC+TGG | + | Chr8:13347243-13347262 | MsG0880042733.01.T01:CDS | 40.0% |
| ! | GCAGATGATTTTGTGTTCTC+TGG | + | Chr8:13347144-13347163 | MsG0880042733.01.T01:CDS | 40.0% |
| !! | ACACTTGTTTCTGACTTGCA+TGG | - | Chr8:13347120-13347139 | None:intergenic | 40.0% |
| !! | ACCAGTTTACCTGGTCTTAA+TGG | + | Chr8:13347225-13347244 | MsG0880042733.01.T01:CDS | 40.0% |
| !! | TTACCTGGTCTTAATGGACT+TGG | + | Chr8:13347231-13347250 | MsG0880042733.01.T01:CDS | 40.0% |
| AGAAGAAGTTGCAGTTTGGG+AGG | - | Chr8:13347043-13347062 | None:intergenic | 45.0% | |
| CTTGGTATATCTGCTGGAAG+AGG | + | Chr8:13347249-13347268 | MsG0880042733.01.T01:CDS | 45.0% | |
| GCATTTGTCACCAGTTTACC+TGG | + | Chr8:13347216-13347235 | MsG0880042733.01.T01:CDS | 45.0% | |
| GTTAAAGGCCAATTGACTGC+TGG | + | Chr8:13347339-13347358 | MsG0880042733.01.T01:CDS | 45.0% | |
| ! | TCTGCTGGAAGAGGAGATTT+TGG | + | Chr8:13347258-13347277 | MsG0880042733.01.T01:CDS | 45.0% |
| CAGCTTCCAAACACTCCTTC+AGG | + | Chr8:13347090-13347109 | MsG0880042733.01.T01:CDS | 50.0% | |
| GACTTGCATGGATAGCCTGA+AGG | - | Chr8:13347108-13347127 | None:intergenic | 50.0% | |
| GCATCAGGATGAGTGAGCAT+TGG | - | Chr8:13347300-13347319 | None:intergenic | 50.0% | |
| !! | GGATAGCCTGAAGGAGTGTT+TGG | - | Chr8:13347099-13347118 | None:intergenic | 50.0% |
| !! | GTGTTCTCTGGCTTTGTACC+TGG | + | Chr8:13347156-13347175 | MsG0880042733.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 13346988 | 13347629 | 13346988 | ID=MsG0880042733.01;Name=MsG0880042733.01 |
| Chr8 | mRNA | 13346988 | 13347629 | 13346988 | ID=MsG0880042733.01.T01;Parent=MsG0880042733.01;Name=MsG0880042733.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|213 |
| Chr8 | exon | 13346988 | 13347629 | 13346988 | ID=MsG0880042733.01.T01:exon:822;Parent=MsG0880042733.01.T01 |
| Chr8 | CDS | 13346988 | 13347629 | 13346988 | ID=MsG0880042733.01.T01:cds;Parent=MsG0880042733.01.T01 |
| Gene Sequence |
| Protein sequence |
