Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045179.01.T01 | KEH20077.1 | 93.023 | 43 | 3 | 0 | 1 | 43 | 1 | 43 | 8.23E-18 | 83.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045179.01.T01 | A0A072TRP0 | 93.023 | 43 | 3 | 0 | 1 | 43 | 1 | 43 | 3.93e-18 | 83.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001187.01 | MsG0880045179.01 | 0.800242 | 1.580222e-48 | 7.321261e-46 |
| MsG0180001771.01 | MsG0880045179.01 | 0.810347 | 1.206095e-50 | 7.252542e-48 |
| MsG0180002259.01 | MsG0880045179.01 | 0.807794 | 4.248113e-50 | 2.388145e-47 |
| MsG0180004765.01 | MsG0880045179.01 | 0.803111 | 4.073785e-49 | 2.029971e-46 |
| MsG0180005113.01 | MsG0880045179.01 | 0.807005 | 6.242339e-50 | 3.437973e-47 |
| MsG0780040514.01 | MsG0880045179.01 | 0.812553 | 4.001802e-51 | 2.552202e-48 |
| MsG0880041858.01 | MsG0880045179.01 | 0.877976 | 4.076665e-69 | 2.091676e-65 |
| MsG0880042733.01 | MsG0880045179.01 | 0.809063 | 2.276850e-50 | 1.323492e-47 |
| MsG0380014221.01 | MsG0880045179.01 | 0.803543 | 3.315328e-49 | 1.670614e-46 |
| MsG0680033933.01 | MsG0880045179.01 | 0.802389 | 5.742980e-49 | 2.809805e-46 |
| MsG0680035655.01 | MsG0880045179.01 | 0.820822 | 5.597540e-53 | 4.476633e-50 |
| MsG0480020748.01 | MsG0880045179.01 | 0.854837 | 9.430770e-62 | 2.134310e-58 |
| MsG0480023591.01 | MsG0880045179.01 | 0.802623 | 5.139414e-49 | 2.529405e-46 |
| MsG0580029941.01 | MsG0880045179.01 | 0.829839 | 4.111159e-55 | 4.258013e-52 |
| MsG0280006592.01 | MsG0880045179.01 | 0.812644 | 3.822477e-51 | 2.443637e-48 |
| MsG0280006966.01 | MsG0880045179.01 | 0.833020 | 6.775860e-56 | 7.706812e-53 |
| MsG0280006967.01 | MsG0880045179.01 | 0.848829 | 4.776332e-60 | 8.877435e-57 |
| MsG0280007515.01 | MsG0880045179.01 | 0.801333 | 9.459319e-49 | 4.505541e-46 |
| MsG0280007884.01 | MsG0880045179.01 | 0.813787 | 2.144591e-51 | 1.413831e-48 |
| MsG0280008390.01 | MsG0880045179.01 | 0.822763 | 1.990709e-53 | 1.681795e-50 |
| MsG0280008391.01 | MsG0880045179.01 | 0.823016 | 1.738149e-53 | 1.478954e-50 |
| MsG0280008392.01 | MsG0880045179.01 | 0.827675 | 1.372299e-54 | 1.333935e-51 |
| MsG0280008395.01 | MsG0880045179.01 | 0.842535 | 2.437417e-58 | 3.706997e-55 |
| MsG0280008396.01 | MsG0880045179.01 | 0.820903 | 5.364352e-53 | 4.299838e-50 |
| MsG0280008397.01 | MsG0880045179.01 | 0.818302 | 2.103786e-52 | 1.568861e-49 |
| MsG0280008899.01 | MsG0880045179.01 | 0.829549 | 4.838559e-55 | 4.967885e-52 |
| MsG0280009920.01 | MsG0880045179.01 | 0.809261 | 2.065312e-50 | 1.206912e-47 |
| MsG0680035683.01 | MsG0880045179.01 | 0.842359 | 2.713093e-58 | 4.103780e-55 |
| MsG0780039940.01 | MsG0880045179.01 | 0.800093 | 1.693961e-48 | 7.818747e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045179.01.T01 | MTR_8g067020 | 93.023 | 43 | 3 | 0 | 1 | 43 | 1 | 43 | 9.97e-22 | 83.6 |
| MsG0880045179.01.T01 | MTR_8g066920 | 45.385 | 130 | 35 | 3 | 18 | 111 | 1 | 130 | 1.14e-17 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGTGGGCTGCCTATTAT+AGG | 0.284888 | 8:+55399989 | MsG0880045179.01.T01:CDS |
| CATTGCTCTCTTTGTGCTGT+TGG | 0.329983 | 8:+55399772 | MsG0880045179.01.T01:CDS |
| CCCACGATGCCTCCTATAAT+AGG | 0.373636 | 8:-55400001 | None:intergenic |
| ATTGCTCTCTTTGTGCTGTT+GGG | 0.382762 | 8:+55399773 | MsG0880045179.01.T01:CDS |
| CTTCCATTTCTTAATGAAGA+AGG | 0.402039 | 8:+55399926 | MsG0880045179.01.T01:CDS |
| GATTCAAACAAAATTTCATA+TGG | 0.405629 | 8:+55399730 | MsG0880045179.01.T01:CDS |
| GATCCTTCTTCATTAAGAAA+TGG | 0.407397 | 8:-55399929 | None:intergenic |
| TGTACCAGGTGAAGGAGGTT+TGG | 0.409408 | 8:+55399970 | MsG0880045179.01.T01:CDS |
| AATGGAAGGTGCTCACCTTC+GGG | 0.412981 | 8:-55399911 | None:intergenic |
| AAATGGAAGGTGCTCACCTT+CGG | 0.419911 | 8:-55399912 | None:intergenic |
| TTTCTTAATGAAGAAGGATC+TGG | 0.427761 | 8:+55399932 | MsG0880045179.01.T01:CDS |
| GGTTGATAGCCGTCTCGATC+AGG | 0.432059 | 8:-55399890 | None:intergenic |
| GTACCAGGTGAAGGAGGTTT+GGG | 0.436790 | 8:+55399971 | MsG0880045179.01.T01:CDS |
| CCAGGTGAAGGAGGTTTGGG+TGG | 0.461957 | 8:+55399974 | MsG0880045179.01.T01:CDS |
| GGAGGACGTGGAGGACTGAC+TGG | 0.463921 | 8:+55399857 | MsG0880045179.01.T01:CDS |
| CCCACGACGCCTCCTACAAT+AGG | 0.464466 | 8:-55400034 | None:intergenic |
| GGAGGACTAGTGCCTATTGT+AGG | 0.493689 | 8:+55400022 | MsG0880045179.01.T01:CDS |
| GTGGATGTTGGTGGAGGACG+TGG | 0.495831 | 8:+55399845 | MsG0880045179.01.T01:CDS |
| GGAGGAGTGCCTAGTGTACC+AGG | 0.502998 | 8:+55399956 | MsG0880045179.01.T01:CDS |
| CCTCCTTCACCTGGTACACT+AGG | 0.503695 | 8:-55399965 | None:intergenic |
| CGTGATTCTGTGGATGTTGG+TGG | 0.519014 | 8:+55399836 | MsG0880045179.01.T01:CDS |
| CAGGTGAAGGAGGTTTGGGT+GGG | 0.540199 | 8:+55399975 | MsG0880045179.01.T01:CDS |
| CCACCCAAACCTCCTTCACC+TGG | 0.566494 | 8:-55399974 | None:intergenic |
| GGTGGGCTGCCTATTATAGG+AGG | 0.567409 | 8:+55399992 | MsG0880045179.01.T01:CDS |
| TCGTGGGAGGACAGCCGTGA+AGG | 0.567702 | 8:+55400083 | MsG0880045179.01.T01:CDS |
| GTGCCTAGTGTACCAGGTGA+AGG | 0.574507 | 8:+55399962 | MsG0880045179.01.T01:CDS |
| AATGAAGAAGGATCTGGAGG+AGG | 0.580725 | 8:+55399938 | MsG0880045179.01.T01:CDS |
| CTTCTTCATTAAGAAATGGA+AGG | 0.581427 | 8:-55399925 | None:intergenic |
| GCCTATTGTAGGAGGCGTCG+TGG | 0.593233 | 8:+55400033 | MsG0880045179.01.T01:CDS |
| GGACTAGTGCCTACTGTAGG+AGG | 0.596151 | 8:+55400058 | MsG0880045179.01.T01:CDS |
| CCTATTGTAGGAGGCGTCGT+GGG | 0.597768 | 8:+55400034 | MsG0880045179.01.T01:CDS |
| AATCTTATACAACTGAGATG+TGG | 0.600420 | 8:-55399711 | None:intergenic |
| ATTGTAGGAGGCGTCGTGGG+AGG | 0.603641 | 8:+55400037 | MsG0880045179.01.T01:CDS |
| AATCGTGATTCTGTGGATGT+TGG | 0.605620 | 8:+55399833 | MsG0880045179.01.T01:CDS |
| CTACGACAATCGTGATTCTG+TGG | 0.607419 | 8:+55399826 | MsG0880045179.01.T01:CDS |
| GATTCTGTGGATGTTGGTGG+AGG | 0.618118 | 8:+55399839 | MsG0880045179.01.T01:CDS |
| CCTAGTGTACCAGGTGAAGG+AGG | 0.618920 | 8:+55399965 | MsG0880045179.01.T01:CDS |
| CTTAATGAAGAAGGATCTGG+AGG | 0.620570 | 8:+55399935 | MsG0880045179.01.T01:CDS |
| CCTATTATAGGAGGCATCGT+GGG | 0.626828 | 8:+55400001 | MsG0880045179.01.T01:CDS |
| GCCTATTATAGGAGGCATCG+TGG | 0.628056 | 8:+55400000 | MsG0880045179.01.T01:CDS |
| ATTATAGGAGGCATCGTGGG+AGG | 0.632352 | 8:+55400004 | MsG0880045179.01.T01:CDS |
| ACTGTAGGAGGCATCGTGGG+AGG | 0.633982 | 8:+55400070 | MsG0880045179.01.T01:CDS |
| GGACTAGTGCCTATTGTAGG+AGG | 0.645107 | 8:+55400025 | MsG0880045179.01.T01:CDS |
| CCCACGATGCCTCCTACAGT+AGG | 0.659459 | 8:-55400067 | None:intergenic |
| CCTACTGTAGGAGGCATCGT+GGG | 0.660575 | 8:+55400067 | MsG0880045179.01.T01:CDS |
| GATGTTGGTGGAGGACGTGG+AGG | 0.664140 | 8:+55399848 | MsG0880045179.01.T01:CDS |
| GGAGGACTAGTGCCTACTGT+AGG | 0.670577 | 8:+55400055 | MsG0880045179.01.T01:CDS |
| GCCTACTGTAGGAGGCATCG+TGG | 0.685025 | 8:+55400066 | MsG0880045179.01.T01:CDS |
| GGTGTCGATCCTGATCGAGA+CGG | 0.686028 | 8:+55399881 | MsG0880045179.01.T01:CDS |
| GGACGTGGAGGACTGACTGG+TGG | 0.698304 | 8:+55399860 | MsG0880045179.01.T01:CDS |
| CGAGACGGCTATCAACCCGA+AGG | 0.711400 | 8:+55399896 | MsG0880045179.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTCAAACAAAATTTCATA+TGG | + | Chr8:55399730-55399749 | MsG0880045179.01.T01:CDS | 20.0% |
| AATCTTATACAACTGAGATG+TGG | - | Chr8:55399714-55399733 | None:intergenic | 30.0% | |
| CTTCCATTTCTTAATGAAGA+AGG | + | Chr8:55399926-55399945 | MsG0880045179.01.T01:CDS | 30.0% | |
| CTTCTTCATTAAGAAATGGA+AGG | - | Chr8:55399928-55399947 | None:intergenic | 30.0% | |
| GATCCTTCTTCATTAAGAAA+TGG | - | Chr8:55399932-55399951 | None:intergenic | 30.0% | |
| TTTCTTAATGAAGAAGGATC+TGG | + | Chr8:55399932-55399951 | MsG0880045179.01.T01:CDS | 30.0% | |
| AATCGTGATTCTGTGGATGT+TGG | + | Chr8:55399833-55399852 | MsG0880045179.01.T01:CDS | 40.0% | |
| CTTAATGAAGAAGGATCTGG+AGG | + | Chr8:55399935-55399954 | MsG0880045179.01.T01:CDS | 40.0% | |
| ! | ATTGCTCTCTTTGTGCTGTT+GGG | + | Chr8:55399773-55399792 | MsG0880045179.01.T01:CDS | 40.0% |
| AATGAAGAAGGATCTGGAGG+AGG | + | Chr8:55399938-55399957 | MsG0880045179.01.T01:CDS | 45.0% | |
| CATTGCTCTCTTTGTGCTGT+TGG | + | Chr8:55399772-55399791 | MsG0880045179.01.T01:CDS | 45.0% | |
| CTACGACAATCGTGATTCTG+TGG | + | Chr8:55399826-55399845 | MsG0880045179.01.T01:CDS | 45.0% | |
| !! | AAATGGAAGGTGCTCACCTT+CGG | - | Chr8:55399915-55399934 | None:intergenic | 45.0% |
| !! | CCTATTATAGGAGGCATCGT+GGG | + | Chr8:55400001-55400020 | MsG0880045179.01.T01:CDS | 45.0% |
| CCCACGATGCCTCCTATAAT+AGG | - | Chr8:55400004-55400023 | None:intergenic | 50.0% | |
| CGTGATTCTGTGGATGTTGG+TGG | + | Chr8:55399836-55399855 | MsG0880045179.01.T01:CDS | 50.0% | |
| GTACCAGGTGAAGGAGGTTT+GGG | + | Chr8:55399971-55399990 | MsG0880045179.01.T01:CDS | 50.0% | |
| TGTACCAGGTGAAGGAGGTT+TGG | + | Chr8:55399970-55399989 | MsG0880045179.01.T01:CDS | 50.0% | |
| ! | GATTCTGTGGATGTTGGTGG+AGG | + | Chr8:55399839-55399858 | MsG0880045179.01.T01:CDS | 50.0% |
| ! | GGACTAGTGCCTATTGTAGG+AGG | + | Chr8:55400025-55400044 | MsG0880045179.01.T01:CDS | 50.0% |
| ! | GGAGGACTAGTGCCTATTGT+AGG | + | Chr8:55400022-55400041 | MsG0880045179.01.T01:CDS | 50.0% |
| !! | AATGGAAGGTGCTCACCTTC+GGG | - | Chr8:55399914-55399933 | None:intergenic | 50.0% |
| !! | ATTATAGGAGGCATCGTGGG+AGG | + | Chr8:55400004-55400023 | MsG0880045179.01.T01:CDS | 50.0% |
| !! | GCCTATTATAGGAGGCATCG+TGG | + | Chr8:55400000-55400019 | MsG0880045179.01.T01:CDS | 50.0% |
| !! | TTGGGTGGGCTGCCTATTAT+AGG | + | Chr8:55399989-55400008 | MsG0880045179.01.T01:CDS | 50.0% |
| CAGGTGAAGGAGGTTTGGGT+GGG | + | Chr8:55399975-55399994 | MsG0880045179.01.T01:CDS | 55.0% | |
| CCTAGTGTACCAGGTGAAGG+AGG | + | Chr8:55399965-55399984 | MsG0880045179.01.T01:CDS | 55.0% | |
| CCTCCTTCACCTGGTACACT+AGG | - | Chr8:55399968-55399987 | None:intergenic | 55.0% | |
| GTGCCTAGTGTACCAGGTGA+AGG | + | Chr8:55399962-55399981 | MsG0880045179.01.T01:CDS | 55.0% | |
| ! | GGACTAGTGCCTACTGTAGG+AGG | + | Chr8:55400058-55400077 | MsG0880045179.01.T01:CDS | 55.0% |
| ! | GGAGGACTAGTGCCTACTGT+AGG | + | Chr8:55400055-55400074 | MsG0880045179.01.T01:CDS | 55.0% |
| !! | CCTACTGTAGGAGGCATCGT+GGG | + | Chr8:55400067-55400086 | MsG0880045179.01.T01:CDS | 55.0% |
| !! | CCTATTGTAGGAGGCGTCGT+GGG | + | Chr8:55400034-55400053 | MsG0880045179.01.T01:CDS | 55.0% |
| !! | GGTGGGCTGCCTATTATAGG+AGG | + | Chr8:55399992-55400011 | MsG0880045179.01.T01:CDS | 55.0% |
| !! | GGTGTCGATCCTGATCGAGA+CGG | + | Chr8:55399881-55399900 | MsG0880045179.01.T01:CDS | 55.0% |
| !! | GGTTGATAGCCGTCTCGATC+AGG | - | Chr8:55399893-55399912 | None:intergenic | 55.0% |
| CCACCCAAACCTCCTTCACC+TGG | - | Chr8:55399977-55399996 | None:intergenic | 60.0% | |
| CCAGGTGAAGGAGGTTTGGG+TGG | + | Chr8:55399974-55399993 | MsG0880045179.01.T01:CDS | 60.0% | |
| CCCACGACGCCTCCTACAAT+AGG | - | Chr8:55400037-55400056 | None:intergenic | 60.0% | |
| CCCACGATGCCTCCTACAGT+AGG | - | Chr8:55400070-55400089 | None:intergenic | 60.0% | |
| CGAGACGGCTATCAACCCGA+AGG | + | Chr8:55399896-55399915 | MsG0880045179.01.T01:CDS | 60.0% | |
| ! | GATGTTGGTGGAGGACGTGG+AGG | + | Chr8:55399848-55399867 | MsG0880045179.01.T01:CDS | 60.0% |
| ! | GTGGATGTTGGTGGAGGACG+TGG | + | Chr8:55399845-55399864 | MsG0880045179.01.T01:CDS | 60.0% |
| !! | ACTGTAGGAGGCATCGTGGG+AGG | + | Chr8:55400070-55400089 | MsG0880045179.01.T01:CDS | 60.0% |
| !! | ATTGTAGGAGGCGTCGTGGG+AGG | + | Chr8:55400037-55400056 | MsG0880045179.01.T01:CDS | 60.0% |
| !! | GCCTACTGTAGGAGGCATCG+TGG | + | Chr8:55400066-55400085 | MsG0880045179.01.T01:CDS | 60.0% |
| !! | GCCTATTGTAGGAGGCGTCG+TGG | + | Chr8:55400033-55400052 | MsG0880045179.01.T01:CDS | 60.0% |
| !! | GGAGGAGTGCCTAGTGTACC+AGG | + | Chr8:55399956-55399975 | MsG0880045179.01.T01:CDS | 60.0% |
| GGACGTGGAGGACTGACTGG+TGG | + | Chr8:55399860-55399879 | MsG0880045179.01.T01:CDS | 65.0% | |
| GGAGGACGTGGAGGACTGAC+TGG | + | Chr8:55399857-55399876 | MsG0880045179.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 55399698 | 55400102 | 55399698 | ID=MsG0880045179.01;Name=MsG0880045179.01 |
| Chr8 | mRNA | 55399698 | 55400102 | 55399698 | ID=MsG0880045179.01.T01;Parent=MsG0880045179.01;Name=MsG0880045179.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|134 |
| Chr8 | exon | 55399698 | 55400102 | 55399698 | ID=MsG0880045179.01.T01:exon:23892;Parent=MsG0880045179.01.T01 |
| Chr8 | CDS | 55399698 | 55400102 | 55399698 | ID=MsG0880045179.01.T01:cds;Parent=MsG0880045179.01.T01 |
| Gene Sequence |
| Protein sequence |
