Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006966.01.T01 | PNX82376.1 | 68.627 | 153 | 48 | 0 | 8 | 160 | 1 | 153 | 1.18E-71 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006966.01.T01 | Q9C7N4 | 43.671 | 158 | 80 | 4 | 8 | 157 | 206 | 362 | 1.30E-35 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000098.01 | MsG0280006966.01 | 0.807906 | 4.020960e-50 | 2.266924e-47 |
| MsG0180000782.01 | MsG0280006966.01 | 0.817353 | 3.446831e-52 | 2.504233e-49 |
| MsG0180003741.01 | MsG0280006966.01 | 0.841845 | 3.710351e-58 | 5.521634e-55 |
| MsG0180004765.01 | MsG0280006966.01 | 0.809737 | 1.631872e-50 | 9.658050e-48 |
| MsG0180005113.01 | MsG0280006966.01 | 0.863936 | 1.743910e-64 | 5.379755e-61 |
| MsG0180005153.01 | MsG0280006966.01 | 0.852269 | 5.156249e-61 | 1.071726e-57 |
| MsG0280006592.01 | MsG0280006966.01 | 0.821683 | 3.544005e-53 | 2.903198e-50 |
| MsG0280006966.01 | MsG0280006967.01 | 0.891328 | 4.390341e-74 | 3.832402e-70 |
| MsG0280006966.01 | MsG0280007515.01 | 0.831997 | 1.215353e-55 | 1.340959e-52 |
| MsG0280006966.01 | MsG0280008390.01 | 0.860641 | 1.793302e-63 | 4.936153e-60 |
| MsG0280006966.01 | MsG0280008391.01 | 0.835400 | 1.715341e-56 | 2.095313e-53 |
| MsG0280006966.01 | MsG0280008392.01 | 0.853971 | 1.678810e-61 | 3.692431e-58 |
| MsG0280006966.01 | MsG0280008395.01 | 0.844288 | 8.296747e-59 | 1.333128e-55 |
| MsG0280006966.01 | MsG0280008397.01 | 0.816460 | 5.467279e-52 | 3.876131e-49 |
| MsG0280006966.01 | MsG0280008899.01 | 0.827861 | 1.237989e-54 | 1.210036e-51 |
| MsG0280006966.01 | MsG0280009920.01 | 0.840581 | 7.976485e-58 | 1.141199e-54 |
| MsG0280006966.01 | MsG0480018200.01 | 0.804604 | 1.994696e-49 | 1.032879e-46 |
| MsG0280006966.01 | MsG0480024022.01 | 0.833283 | 5.828750e-56 | 6.681389e-53 |
| MsG0280006966.01 | MsG0680030328.01 | 0.813660 | 2.287230e-51 | 1.502694e-48 |
| MsG0280006966.01 | MsG0680030329.01 | 0.835378 | 1.737787e-56 | 2.121344e-53 |
| MsG0280006966.01 | MsG0680031428.01 | 0.829573 | 4.774294e-55 | 4.905429e-52 |
| MsG0280006966.01 | MsG0680031959.01 | 0.826879 | 2.128873e-54 | 2.021909e-51 |
| MsG0280006966.01 | MsG0780039940.01 | 0.807810 | 4.213395e-50 | 2.369627e-47 |
| MsG0280006966.01 | MsG0780040286.01 | 0.804845 | 1.776129e-49 | 9.255111e-47 |
| MsG0280006966.01 | MsG0780040287.01 | 0.824771 | 6.739772e-54 | 6.027979e-51 |
| MsG0280006966.01 | MsG0780040430.01 | 0.825469 | 4.609654e-54 | 4.205093e-51 |
| MsG0280006966.01 | MsG0780040431.01 | 0.800746 | 1.247208e-48 | 5.852787e-46 |
| MsG0280006966.01 | MsG0780040846.01 | 0.807436 | 5.060005e-50 | 2.818141e-47 |
| MsG0280006966.01 | MsG0780041259.01 | 0.811171 | 8.000596e-51 | 4.917523e-48 |
| MsG0280006966.01 | MsG0880041834.01 | 0.816938 | 4.271533e-52 | 3.068675e-49 |
| MsG0280006966.01 | MsG0880041858.01 | 0.829756 | 4.308685e-55 | 4.451466e-52 |
| MsG0280006966.01 | MsG0880042732.01 | 0.807174 | 5.750150e-50 | 3.180848e-47 |
| MsG0280006966.01 | MsG0880042733.01 | 0.804756 | 1.854379e-49 | 9.640393e-47 |
| MsG0280006966.01 | MsG0880042734.01 | 0.801240 | 9.885248e-49 | 4.697386e-46 |
| MsG0280006966.01 | MsG0880043037.01 | 0.811388 | 7.178362e-51 | 4.437496e-48 |
| MsG0280006966.01 | MsG0880045177.01 | 0.821288 | 4.373367e-53 | 3.543041e-50 |
| MsG0280006966.01 | MsG0880045179.01 | 0.833020 | 6.775860e-56 | 7.706812e-53 |
| MsG0280006966.01 | MsG0880045585.01 | 0.830565 | 2.734603e-55 | 2.891707e-52 |
| MsG0280006966.01 | MsG0880046636.01 | 0.805189 | 1.505294e-49 | 7.912921e-47 |
| MsG0280006966.01 | MsG0880047135.01 | 0.835505 | 1.613567e-56 | 1.977112e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006966.01.T01 | MTR_2g017875 | 61.589 | 151 | 58 | 0 | 7 | 157 | 210 | 360 | 8.05e-65 | 202 |
| MsG0280006966.01.T01 | MTR_2g017955 | 53.595 | 153 | 69 | 2 | 7 | 157 | 207 | 359 | 1.01e-47 | 159 |
| MsG0280006966.01.T01 | MTR_2g017965 | 51.282 | 156 | 74 | 2 | 7 | 160 | 208 | 363 | 2.09e-47 | 158 |
| MsG0280006966.01.T01 | MTR_3g023220 | 50.993 | 151 | 70 | 2 | 7 | 157 | 206 | 352 | 1.90e-46 | 155 |
| MsG0280006966.01.T01 | MTR_2g017895 | 57.031 | 128 | 55 | 0 | 33 | 160 | 142 | 269 | 1.29e-45 | 150 |
| MsG0280006966.01.T01 | MTR_2g017925 | 50.968 | 155 | 69 | 4 | 8 | 160 | 105 | 254 | 1.99e-44 | 147 |
| MsG0280006966.01.T01 | MTR_2g017925 | 50.968 | 155 | 69 | 4 | 8 | 160 | 208 | 357 | 2.27e-43 | 147 |
| MsG0280006966.01.T01 | MTR_8g087890 | 47.500 | 160 | 74 | 5 | 7 | 157 | 204 | 362 | 9.96e-40 | 137 |
| MsG0280006966.01.T01 | MTR_2g018015 | 62.500 | 120 | 32 | 3 | 44 | 161 | 196 | 304 | 1.90e-39 | 135 |
| MsG0280006966.01.T01 | MTR_8g087870 | 42.105 | 152 | 84 | 4 | 7 | 157 | 202 | 350 | 1.32e-36 | 129 |
| MsG0280006966.01.T01 | MTR_2g017915 | 44.156 | 154 | 65 | 2 | 8 | 160 | 208 | 341 | 4.80e-35 | 125 |
| MsG0280006966.01.T01 | MTR_3g005650 | 40.127 | 157 | 83 | 4 | 9 | 157 | 203 | 356 | 6.78e-32 | 117 |
| MsG0280006966.01.T01 | MTR_0565s0010 | 41.139 | 158 | 83 | 5 | 9 | 157 | 208 | 364 | 3.70e-31 | 115 |
| MsG0280006966.01.T01 | MTR_3g024510 | 39.873 | 158 | 85 | 5 | 9 | 157 | 213 | 369 | 8.12e-31 | 114 |
| MsG0280006966.01.T01 | MTR_7g116520 | 35.821 | 134 | 78 | 2 | 7 | 132 | 209 | 342 | 2.44e-23 | 94.4 |
| MsG0280006966.01.T01 | MTR_4g019880 | 33.537 | 164 | 85 | 5 | 8 | 155 | 208 | 363 | 3.32e-23 | 94.0 |
| MsG0280006966.01.T01 | MTR_7g116500 | 34.194 | 155 | 92 | 3 | 9 | 155 | 210 | 362 | 7.81e-23 | 93.2 |
| MsG0280006966.01.T01 | MTR_3g098270 | 36.250 | 160 | 89 | 6 | 8 | 157 | 212 | 368 | 1.93e-22 | 92.0 |
| MsG0280006966.01.T01 | MTR_5g078210 | 34.752 | 141 | 84 | 3 | 7 | 140 | 200 | 339 | 8.79e-22 | 90.1 |
| MsG0280006966.01.T01 | MTR_3g005660 | 35.625 | 160 | 89 | 6 | 8 | 156 | 212 | 368 | 9.89e-22 | 90.1 |
| MsG0280006966.01.T01 | MTR_7g116570 | 33.582 | 134 | 77 | 3 | 7 | 130 | 209 | 340 | 3.17e-21 | 88.6 |
| MsG0280006966.01.T01 | MTR_7g008060 | 34.328 | 134 | 80 | 2 | 7 | 132 | 195 | 328 | 3.54e-21 | 88.6 |
| MsG0280006966.01.T01 | MTR_1g079540 | 34.109 | 129 | 77 | 2 | 7 | 127 | 206 | 334 | 2.02e-20 | 86.7 |
| MsG0280006966.01.T01 | MTR_5g009100 | 29.560 | 159 | 102 | 4 | 7 | 156 | 220 | 377 | 1.44e-19 | 84.3 |
| MsG0280006966.01.T01 | MTR_4g035353 | 33.125 | 160 | 94 | 6 | 8 | 157 | 206 | 362 | 2.77e-19 | 83.2 |
| MsG0280006966.01.T01 | MTR_6g021530 | 33.088 | 136 | 79 | 3 | 7 | 132 | 205 | 338 | 1.35e-18 | 81.3 |
| MsG0280006966.01.T01 | MTR_1g069175 | 32.836 | 134 | 81 | 3 | 9 | 133 | 212 | 345 | 1.35e-18 | 81.6 |
| MsG0280006966.01.T01 | MTR_0127s0040 | 32.278 | 158 | 97 | 3 | 7 | 157 | 83 | 237 | 3.85e-18 | 78.6 |
| MsG0280006966.01.T01 | MTR_1g024890 | 31.783 | 129 | 80 | 2 | 7 | 127 | 207 | 335 | 5.38e-18 | 79.7 |
| MsG0280006966.01.T01 | MTR_4g122200 | 34.783 | 161 | 89 | 7 | 9 | 157 | 211 | 367 | 2.62e-17 | 77.8 |
| MsG0280006966.01.T01 | MTR_5g093090 | 31.210 | 157 | 96 | 4 | 7 | 155 | 207 | 359 | 3.77e-17 | 77.4 |
| MsG0280006966.01.T01 | MTR_7g116510 | 32.593 | 135 | 78 | 4 | 7 | 130 | 200 | 332 | 4.74e-17 | 77.0 |
| MsG0280006966.01.T01 | MTR_2g017945 | 50.704 | 71 | 35 | 0 | 87 | 157 | 88 | 158 | 6.67e-17 | 73.9 |
| MsG0280006966.01.T01 | MTR_4g108690 | 37.778 | 135 | 73 | 4 | 9 | 134 | 205 | 337 | 1.32e-16 | 75.9 |
| MsG0280006966.01.T01 | MTR_3g435540 | 34.328 | 134 | 79 | 3 | 9 | 134 | 201 | 333 | 1.98e-16 | 75.5 |
| MsG0280006966.01.T01 | MTR_1g079530 | 30.201 | 149 | 98 | 3 | 7 | 152 | 204 | 349 | 4.75e-16 | 74.3 |
| MsG0280006966.01.T01 | MTR_3g052490 | 29.936 | 157 | 98 | 4 | 7 | 155 | 211 | 363 | 1.48e-15 | 72.8 |
| MsG0280006966.01.T01 | MTR_2g033930 | 30.818 | 159 | 98 | 5 | 9 | 157 | 206 | 362 | 3.16e-15 | 72.0 |
| MsG0280006966.01.T01 | MTR_3g052580 | 29.012 | 162 | 103 | 4 | 7 | 160 | 205 | 362 | 3.20e-15 | 72.0 |
| MsG0280006966.01.T01 | MTR_3g052580 | 29.012 | 162 | 103 | 4 | 7 | 160 | 206 | 363 | 3.62e-15 | 72.0 |
| MsG0280006966.01.T01 | MTR_2g033920 | 31.056 | 161 | 95 | 6 | 9 | 157 | 211 | 367 | 8.50e-14 | 68.2 |
| MsG0280006966.01.T01 | MTR_3g021460 | 33.121 | 157 | 85 | 4 | 8 | 157 | 206 | 349 | 1.18e-13 | 67.4 |
| MsG0280006966.01.T01 | MTR_5g084750 | 31.884 | 138 | 84 | 4 | 7 | 134 | 203 | 340 | 1.28e-13 | 67.4 |
| MsG0280006966.01.T01 | MTR_3g052480 | 29.814 | 161 | 96 | 5 | 7 | 156 | 212 | 366 | 1.37e-13 | 67.4 |
| MsG0280006966.01.T01 | MTR_2g461580 | 32.911 | 158 | 86 | 6 | 7 | 157 | 205 | 349 | 6.52e-13 | 65.5 |
| MsG0280006966.01.T01 | MTR_1g025780 | 28.462 | 130 | 84 | 3 | 7 | 127 | 213 | 342 | 1.20e-12 | 64.7 |
| MsG0280006966.01.T01 | MTR_8g074560 | 30.000 | 130 | 79 | 5 | 7 | 127 | 213 | 339 | 2.38e-12 | 63.9 |
| MsG0280006966.01.T01 | MTR_8g079050 | 28.049 | 164 | 104 | 6 | 4 | 155 | 212 | 373 | 3.07e-12 | 63.5 |
| MsG0280006966.01.T01 | MTR_8g022790 | 27.044 | 159 | 103 | 5 | 7 | 157 | 207 | 360 | 5.64e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006966.01.T01 | AT1G29670 | 43.671 | 158 | 80 | 4 | 8 | 157 | 206 | 362 | 1.33e-36 | 129 |
| MsG0280006966.01.T01 | AT1G29670 | 43.671 | 158 | 80 | 4 | 8 | 157 | 274 | 430 | 5.61e-36 | 129 |
| MsG0280006966.01.T01 | AT1G29660 | 40.252 | 159 | 85 | 4 | 8 | 157 | 206 | 363 | 2.60e-33 | 120 |
| MsG0280006966.01.T01 | AT4G18970 | 41.772 | 158 | 82 | 5 | 9 | 157 | 204 | 360 | 1.56e-30 | 113 |
| MsG0280006966.01.T01 | AT4G18970 | 41.772 | 158 | 82 | 5 | 9 | 157 | 204 | 360 | 3.83e-30 | 113 |
| MsG0280006966.01.T01 | AT5G45670 | 40.764 | 157 | 83 | 5 | 9 | 156 | 205 | 360 | 5.89e-30 | 112 |
| MsG0280006966.01.T01 | AT1G71691 | 36.538 | 156 | 88 | 4 | 9 | 156 | 128 | 280 | 3.40e-29 | 108 |
| MsG0280006966.01.T01 | AT1G71691 | 36.538 | 156 | 88 | 4 | 9 | 156 | 229 | 381 | 2.77e-28 | 108 |
| MsG0280006966.01.T01 | AT1G33811 | 36.025 | 161 | 87 | 6 | 9 | 156 | 211 | 368 | 5.76e-28 | 107 |
| MsG0280006966.01.T01 | AT1G71250 | 35.443 | 158 | 89 | 6 | 8 | 155 | 216 | 370 | 6.07e-23 | 93.6 |
| MsG0280006966.01.T01 | AT4G30140 | 35.099 | 151 | 90 | 4 | 8 | 155 | 201 | 346 | 1.99e-22 | 91.7 |
| MsG0280006966.01.T01 | AT5G08460 | 35.185 | 162 | 91 | 6 | 8 | 157 | 225 | 384 | 3.12e-22 | 91.7 |
| MsG0280006966.01.T01 | AT5G33370 | 31.169 | 154 | 96 | 3 | 7 | 152 | 205 | 356 | 4.71e-22 | 90.9 |
| MsG0280006966.01.T01 | AT3G04290 | 30.519 | 154 | 97 | 3 | 7 | 152 | 204 | 355 | 1.18e-20 | 87.0 |
| MsG0280006966.01.T01 | AT2G19010 | 33.548 | 155 | 95 | 3 | 7 | 157 | 197 | 347 | 1.33e-19 | 84.0 |
| MsG0280006966.01.T01 | AT2G19010 | 33.548 | 155 | 95 | 3 | 7 | 157 | 227 | 377 | 1.61e-19 | 84.3 |
| MsG0280006966.01.T01 | AT2G19050 | 32.903 | 155 | 96 | 3 | 7 | 157 | 198 | 348 | 1.62e-19 | 84.0 |
| MsG0280006966.01.T01 | AT2G19060 | 37.692 | 130 | 75 | 4 | 7 | 130 | 197 | 326 | 5.90e-18 | 79.7 |
| MsG0280006966.01.T01 | AT5G37690 | 32.090 | 134 | 83 | 3 | 8 | 134 | 195 | 327 | 1.58e-17 | 78.6 |
| MsG0280006966.01.T01 | AT5G37690 | 32.090 | 134 | 83 | 3 | 8 | 134 | 203 | 335 | 1.93e-17 | 78.2 |
| MsG0280006966.01.T01 | AT4G28780 | 33.582 | 134 | 80 | 3 | 8 | 132 | 206 | 339 | 8.80e-17 | 76.6 |
| MsG0280006966.01.T01 | AT5G15720 | 31.447 | 159 | 97 | 4 | 9 | 157 | 176 | 332 | 1.06e-16 | 75.9 |
| MsG0280006966.01.T01 | AT5G15720 | 31.447 | 159 | 97 | 4 | 9 | 157 | 207 | 363 | 1.23e-16 | 75.9 |
| MsG0280006966.01.T01 | AT5G18430 | 29.932 | 147 | 84 | 5 | 9 | 138 | 206 | 350 | 7.36e-16 | 73.9 |
| MsG0280006966.01.T01 | AT1G74460 | 32.353 | 136 | 83 | 3 | 7 | 134 | 199 | 333 | 5.64e-15 | 71.2 |
| MsG0280006966.01.T01 | AT2G03980 | 28.378 | 148 | 94 | 3 | 7 | 142 | 212 | 359 | 2.09e-14 | 69.7 |
| MsG0280006966.01.T01 | AT2G03980 | 28.378 | 148 | 94 | 3 | 7 | 142 | 212 | 359 | 2.09e-14 | 69.7 |
| MsG0280006966.01.T01 | AT2G03980 | 28.378 | 148 | 94 | 3 | 7 | 142 | 212 | 359 | 2.09e-14 | 69.7 |
| MsG0280006966.01.T01 | AT2G03980 | 28.378 | 148 | 94 | 3 | 7 | 142 | 212 | 359 | 2.09e-14 | 69.7 |
| MsG0280006966.01.T01 | AT2G04570 | 33.333 | 138 | 82 | 4 | 7 | 134 | 201 | 338 | 7.10e-14 | 68.2 |
| MsG0280006966.01.T01 | AT2G23540 | 30.147 | 136 | 85 | 4 | 9 | 134 | 230 | 365 | 1.81e-13 | 67.0 |
| MsG0280006966.01.T01 | AT4G16233 | 32.576 | 132 | 74 | 5 | 40 | 160 | 5 | 132 | 2.92e-13 | 63.5 |
| MsG0280006966.01.T01 | AT3G50400 | 31.915 | 141 | 81 | 6 | 7 | 134 | 214 | 352 | 3.58e-13 | 66.2 |
| MsG0280006966.01.T01 | AT5G63170 | 34.646 | 127 | 68 | 5 | 7 | 127 | 202 | 319 | 4.41e-13 | 65.9 |
| MsG0280006966.01.T01 | AT1G20132 | 33.077 | 130 | 77 | 5 | 8 | 127 | 235 | 364 | 1.03e-12 | 65.1 |
| MsG0280006966.01.T01 | AT3G53100 | 32.609 | 138 | 83 | 4 | 7 | 134 | 202 | 339 | 1.85e-12 | 64.3 |
| MsG0280006966.01.T01 | AT4G26790 | 31.724 | 145 | 87 | 4 | 9 | 141 | 204 | 348 | 6.68e-12 | 62.8 |
| MsG0280006966.01.T01 | AT4G26790 | 31.724 | 145 | 87 | 4 | 9 | 141 | 204 | 348 | 6.68e-12 | 62.8 |
| MsG0280006966.01.T01 | AT5G41890 | 29.651 | 172 | 90 | 7 | 7 | 155 | 209 | 372 | 3.36e-11 | 60.8 |
| MsG0280006966.01.T01 | AT1G73610 | 31.496 | 127 | 71 | 5 | 7 | 127 | 209 | 325 | 9.45e-11 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGTCTTGACTTAATTTA+TGG | 0.077141 | 2:+8755614 | None:intergenic |
| GGACAGTCTTTGTATGATTT+TGG | 0.142004 | 2:-8756315 | MsG0280006966.01.T01:CDS |
| ACAAAGATAGATTTAGAATT+TGG | 0.163926 | 2:+8756129 | None:intergenic |
| AAAACGTAAACATTTCTATT+GGG | 0.291264 | 2:+8755756 | None:intergenic |
| TAGATTTAGAATTTGGATAT+CGG | 0.354893 | 2:+8756136 | None:intergenic |
| CATGTTGGAATTGGCCTTAT+AGG | 0.367186 | 2:-8756276 | MsG0280006966.01.T01:CDS |
| CTTACCAAGTGATTCGTCTA+TGG | 0.378684 | 2:+8756089 | None:intergenic |
| AAACTCGTGCATGTTGGAAT+TGG | 0.402053 | 2:-8756285 | MsG0280006966.01.T01:CDS |
| TTGGGGCATGGAGTTGAATC+AGG | 0.418156 | 2:+8755774 | None:intergenic |
| TGTTGTCCAGTGAGGTCAGA+TGG | 0.445100 | 2:-8755807 | MsG0280006966.01.T01:CDS |
| ATCTTTGTAAATAGTACTGC+TGG | 0.460270 | 2:-8756114 | MsG0280006966.01.T01:CDS |
| TCCATTGGATGGGTCGTCCC+TGG | 0.471406 | 2:+8755660 | None:intergenic |
| AATGTGGAGTGCAACCTATA+AGG | 0.471432 | 2:+8756262 | None:intergenic |
| TATGATAGTTCTTCTGCTCC+AGG | 0.488195 | 2:-8755678 | MsG0280006966.01.T01:CDS |
| AAACGTAAACATTTCTATTG+GGG | 0.544555 | 2:+8755757 | None:intergenic |
| CACATCCCATCTGACCTCAC+TGG | 0.554719 | 2:+8755801 | None:intergenic |
| GAGAAAGTTCACAGCTGATG+TGG | 0.560323 | 2:+8755716 | None:intergenic |
| GCAAGAAAACTCGTGCATGT+TGG | 0.562092 | 2:-8756291 | MsG0280006966.01.T01:CDS |
| ATTGCTACTCGAGGGACAGA+TGG | 0.569780 | 2:-8756237 | MsG0280006966.01.T01:CDS |
| TCTCAAACTTAGATCTCTAG+TGG | 0.570387 | 2:-8756172 | MsG0280006966.01.T01:CDS |
| TAAACATTTCTATTGGGGCA+TGG | 0.572507 | 2:+8755762 | None:intergenic |
| GTTGTCCAGTGAGGTCAGAT+GGG | 0.580131 | 2:-8755806 | MsG0280006966.01.T01:CDS |
| ATGATAGTTCTTCTGCTCCA+GGG | 0.599199 | 2:-8755677 | MsG0280006966.01.T01:CDS |
| GGCTCCATAGACGAATCACT+TGG | 0.607025 | 2:-8756093 | MsG0280006966.01.T01:intron |
| CACATTCAATTGCTACTCGA+GGG | 0.607858 | 2:-8756245 | MsG0280006966.01.T01:CDS |
| CCACATTCAATTGCTACTCG+AGG | 0.609254 | 2:-8756246 | MsG0280006966.01.T01:CDS |
| TCTGACCTCACTGGACAACA+AGG | 0.616362 | 2:+8755810 | None:intergenic |
| TCCAGGGACGACCCATCCAA+TGG | 0.625845 | 2:-8755661 | MsG0280006966.01.T01:CDS |
| CCTCGAGTAGCAATTGAATG+TGG | 0.637737 | 2:+8756246 | None:intergenic |
| AGAAAGTTCACAGCTGATGT+GGG | 0.668712 | 2:+8755717 | None:intergenic |
| GTGCTCCTTGTTGTCCAGTG+AGG | 0.670118 | 2:-8755815 | MsG0280006966.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACGTAAACATTTCTAT+TGG | + | Chr2:8756204-8756223 | None:intergenic | 20.0% |
| !! | AAAACGTAAACATTTCTATT+GGG | + | Chr2:8756203-8756222 | None:intergenic | 20.0% |
| !! | GATGTATCTAATTCAAAAAT+TGG | + | Chr2:8755989-8756008 | None:intergenic | 20.0% |
| !!! | AATGTTTACGTTTTTTATGA+TGG | - | Chr2:8756209-8756228 | MsG0280006966.01.T01:CDS | 20.0% |
| !!! | ACAAAGATAGATTTAGAATT+TGG | + | Chr2:8755830-8755849 | None:intergenic | 20.0% |
| !!! | ACTATGATATTTTGTATTTC+AGG | - | Chr2:8756104-8756123 | MsG0280006966.01.T01:CDS | 20.0% |
| !!! | TAGATTTAGAATTTGGATAT+CGG | + | Chr2:8755823-8755842 | None:intergenic | 20.0% |
| ! | AAACGTAAACATTTCTATTG+GGG | + | Chr2:8756202-8756221 | None:intergenic | 25.0% |
| !!! | TTGTTTTATATCCATTGGAT+GGG | + | Chr2:8756309-8756328 | None:intergenic | 25.0% |
| ATCTTTGTAAATAGTACTGC+TGG | - | Chr2:8755842-8755861 | MsG0280006966.01.T01:intron | 30.0% | |
| TGTTGACAAAACTTTCTCTT+AGG | - | Chr2:8756015-8756034 | MsG0280006966.01.T01:intron | 30.0% | |
| ! | ACAGTCTTTGTATGATTTTG+GGG | - | Chr2:8755643-8755662 | MsG0280006966.01.T01:CDS | 30.0% |
| ! | GACAGTCTTTGTATGATTTT+GGG | - | Chr2:8755642-8755661 | MsG0280006966.01.T01:CDS | 30.0% |
| !! | GCAAGTTGTTTTATATCCAT+TGG | + | Chr2:8756314-8756333 | None:intergenic | 30.0% |
| !! | GTTGTTTTATATCCATTGGA+TGG | + | Chr2:8756310-8756329 | None:intergenic | 30.0% |
| AAACTTTCTCTTAGGATCGA+AGG | - | Chr2:8756023-8756042 | MsG0280006966.01.T01:intron | 35.0% | |
| AACTTTCTCTTAGGATCGAA+GGG | - | Chr2:8756024-8756043 | MsG0280006966.01.T01:intron | 35.0% | |
| TAAACATTTCTATTGGGGCA+TGG | + | Chr2:8756197-8756216 | None:intergenic | 35.0% | |
| TCTCAAACTTAGATCTCTAG+TGG | - | Chr2:8755784-8755803 | MsG0280006966.01.T01:CDS | 35.0% | |
| ! | GGACAGTCTTTGTATGATTT+TGG | - | Chr2:8755641-8755660 | MsG0280006966.01.T01:CDS | 35.0% |
| AAACTCGTGCATGTTGGAAT+TGG | - | Chr2:8755671-8755690 | MsG0280006966.01.T01:CDS | 40.0% | |
| AATGTGGAGTGCAACCTATA+AGG | + | Chr2:8755697-8755716 | None:intergenic | 40.0% | |
| AGAAAGTTCACAGCTGATGT+GGG | + | Chr2:8756242-8756261 | None:intergenic | 40.0% | |
| ATGATAGTTCTTCTGCTCCA+GGG | - | Chr2:8756279-8756298 | MsG0280006966.01.T01:CDS | 40.0% | |
| CACATTCAATTGCTACTCGA+GGG | - | Chr2:8755711-8755730 | MsG0280006966.01.T01:CDS | 40.0% | |
| CTTACCAAGTGATTCGTCTA+TGG | + | Chr2:8755870-8755889 | None:intergenic | 40.0% | |
| TATGATAGTTCTTCTGCTCC+AGG | - | Chr2:8756278-8756297 | MsG0280006966.01.T01:CDS | 40.0% | |
| !! | CATGTTGGAATTGGCCTTAT+AGG | - | Chr2:8755680-8755699 | MsG0280006966.01.T01:CDS | 40.0% |
| CCACATTCAATTGCTACTCG+AGG | - | Chr2:8755710-8755729 | MsG0280006966.01.T01:CDS | 45.0% | |
| CCTCGAGTAGCAATTGAATG+TGG | + | Chr2:8755713-8755732 | None:intergenic | 45.0% | |
| GAGAAAGTTCACAGCTGATG+TGG | + | Chr2:8756243-8756262 | None:intergenic | 45.0% | |
| GCAAGAAAACTCGTGCATGT+TGG | - | Chr2:8755665-8755684 | MsG0280006966.01.T01:CDS | 45.0% | |
| ATTGCTACTCGAGGGACAGA+TGG | - | Chr2:8755719-8755738 | MsG0280006966.01.T01:CDS | 50.0% | |
| GGCTCCATAGACGAATCACT+TGG | - | Chr2:8755863-8755882 | MsG0280006966.01.T01:intron | 50.0% | |
| GTTGTCCAGTGAGGTCAGAT+GGG | - | Chr2:8756150-8756169 | MsG0280006966.01.T01:CDS | 50.0% | |
| TCTGACCTCACTGGACAACA+AGG | + | Chr2:8756149-8756168 | None:intergenic | 50.0% | |
| TGTTGTCCAGTGAGGTCAGA+TGG | - | Chr2:8756149-8756168 | MsG0280006966.01.T01:CDS | 50.0% | |
| TTGGGGCATGGAGTTGAATC+AGG | + | Chr2:8756185-8756204 | None:intergenic | 50.0% | |
| CACATCCCATCTGACCTCAC+TGG | + | Chr2:8756158-8756177 | None:intergenic | 55.0% | |
| ! | GTGCTCCTTGTTGTCCAGTG+AGG | - | Chr2:8756141-8756160 | MsG0280006966.01.T01:CDS | 55.0% |
| TCCAGGGACGACCCATCCAA+TGG | - | Chr2:8756295-8756314 | MsG0280006966.01.T01:CDS | 60.0% | |
| TCCATTGGATGGGTCGTCCC+TGG | + | Chr2:8756299-8756318 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 8755626 | 8756352 | 8755626 | ID=MsG0280006966.01;Name=MsG0280006966.01 |
| Chr2 | mRNA | 8755626 | 8756352 | 8755626 | ID=MsG0280006966.01.T01;Parent=MsG0280006966.01;Name=MsG0280006966.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|161 |
| Chr2 | exon | 8756094 | 8756352 | 8756094 | ID=MsG0280006966.01.T01:exon:2532;Parent=MsG0280006966.01.T01 |
| Chr2 | exon | 8755626 | 8755852 | 8755626 | ID=MsG0280006966.01.T01:exon:2531;Parent=MsG0280006966.01.T01 |
| Chr2 | CDS | 8756094 | 8756352 | 8756094 | ID=MsG0280006966.01.T01:cds;Parent=MsG0280006966.01.T01 |
| Chr2 | CDS | 8755626 | 8755852 | 8755626 | ID=MsG0280006966.01.T01:cds;Parent=MsG0280006966.01.T01 |
| Gene Sequence |
| Protein sequence |
