Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046636.01.T01 | XP_003609161.1 | 91.718 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 0 | 593 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046636.01.T01 | P94111 | 43.974 | 307 | 156 | 8 | 31 | 329 | 27 | 325 | 1.94E-70 | 225 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046636.01.T01 | G7JT36 | 91.718 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 0.0 | 593 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0880046636.01 | 0.837217 | 5.919743e-57 | 7.636948e-54 |
| MsG0880045585.01 | MsG0880046636.01 | 0.824858 | 6.427318e-54 | 5.762750e-51 |
| MsG0880045586.01 | MsG0880046636.01 | 0.808659 | 2.777936e-50 | 1.597474e-47 |
| MsG0880046580.01 | MsG0880046636.01 | 0.987688 | 3.595420e-171 | 1.924618e-164 |
| MsG0880046636.01 | MsG0880046942.01 | 0.836291 | 1.020025e-56 | 1.279985e-53 |
| MsG0180003741.01 | MsG0880046636.01 | 0.813111 | 3.020314e-51 | 1.954735e-48 |
| MsG0180004765.01 | MsG0880046636.01 | 0.803398 | 3.553361e-49 | 1.783836e-46 |
| MsG0180005153.01 | MsG0880046636.01 | 0.803154 | 3.990871e-49 | 1.990970e-46 |
| MsG0780040286.01 | MsG0880046636.01 | 0.811345 | 7.332547e-51 | 4.527532e-48 |
| MsG0780040287.01 | MsG0880046636.01 | 0.812808 | 3.518624e-51 | 2.258906e-48 |
| MsG0780040432.01 | MsG0880046636.01 | 0.818249 | 2.162924e-52 | 1.610535e-49 |
| MsG0780040966.01 | MsG0880046636.01 | 0.807696 | 4.455593e-50 | 2.498317e-47 |
| MsG0780041259.01 | MsG0880046636.01 | 0.807642 | 4.575846e-50 | 2.562127e-47 |
| MsG0880041834.01 | MsG0880046636.01 | 0.817836 | 2.681973e-52 | 1.974638e-49 |
| MsG0880041858.01 | MsG0880046636.01 | 0.814463 | 1.521070e-51 | 1.021339e-48 |
| MsG0880042442.01 | MsG0880046636.01 | 0.826424 | 2.733716e-54 | 2.562771e-51 |
| MsG0880042734.01 | MsG0880046636.01 | 0.808703 | 2.718269e-50 | 1.564995e-47 |
| MsG0880043480.01 | MsG0880046636.01 | 0.807947 | 3.941421e-50 | 2.224572e-47 |
| MsG0380011501.01 | MsG0880046636.01 | 0.803649 | 3.151195e-49 | 1.592140e-46 |
| MsG0680031707.01 | MsG0880046636.01 | 0.817239 | 3.655122e-52 | 2.647361e-49 |
| MsG0480018909.01 | MsG0880046636.01 | 0.836417 | 9.471312e-57 | 1.192883e-53 |
| MsG0380017869.01 | MsG0880046636.01 | 0.823938 | 1.057933e-53 | 9.239012e-51 |
| MsG0580029417.01 | MsG0880046636.01 | 0.809155 | 2.176064e-50 | 1.268040e-47 |
| MsG0580029418.01 | MsG0880046636.01 | 0.803927 | 2.759024e-49 | 1.404114e-46 |
| MsG0280006966.01 | MsG0880046636.01 | 0.805189 | 1.505294e-49 | 7.912921e-47 |
| MsG0280006967.01 | MsG0880046636.01 | 0.805025 | 1.628841e-49 | 8.526390e-47 |
| MsG0280007414.01 | MsG0880046636.01 | 0.836247 | 1.046475e-56 | 1.311453e-53 |
| MsG0280007547.01 | MsG0880046636.01 | 0.828156 | 1.051243e-54 | 1.036401e-51 |
| MsG0280009920.01 | MsG0880046636.01 | 0.803722 | 3.043438e-49 | 1.540585e-46 |
| MsG0680035658.01 | MsG0880046636.01 | 0.846181 | 2.551573e-59 | 4.355236e-56 |
| MsG0680035683.01 | MsG0880046636.01 | 0.817317 | 3.510349e-52 | 2.547908e-49 |
| MsG0780038401.01 | MsG0880046636.01 | 0.803940 | 2.742947e-49 | 1.396380e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046635.01 | MsG0880046636.01 | PPI |
| MsG0880046634.01 | MsG0880046636.01 | PPI |
| MsG0880046636.01 | MsG0280009252.01 | PPI |
| MsG0880046580.01 | MsG0880046636.01 | PPI |
| MsG0880046579.01 | MsG0880046636.01 | PPI |
| MsG0880046636.01 | MsG0880046580.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046636.01.T01 | MTR_4g112630 | 91.718 | 326 | 25 | 2 | 7 | 331 | 2 | 326 | 0.0 | 593 |
| MsG0880046636.01.T01 | MTR_4g112650 | 79.403 | 335 | 56 | 4 | 7 | 330 | 2 | 334 | 0.0 | 521 |
| MsG0880046636.01.T01 | MTR_4g112620 | 77.844 | 334 | 36 | 3 | 1 | 330 | 1 | 300 | 0.0 | 505 |
| MsG0880046636.01.T01 | MTR_4g112670 | 74.854 | 342 | 65 | 6 | 1 | 329 | 1 | 334 | 7.84e-179 | 498 |
| MsG0880046636.01.T01 | MTR_2g058450 | 54.341 | 311 | 127 | 4 | 30 | 329 | 28 | 334 | 1.93e-107 | 317 |
| MsG0880046636.01.T01 | MTR_0003s0530 | 70.811 | 185 | 38 | 4 | 149 | 321 | 1 | 181 | 3.97e-82 | 248 |
| MsG0880046636.01.T01 | MTR_7g490330 | 43.448 | 290 | 151 | 6 | 42 | 330 | 47 | 324 | 4.34e-73 | 229 |
| MsG0880046636.01.T01 | MTR_2g058410 | 52.766 | 235 | 98 | 3 | 106 | 330 | 1 | 232 | 2.76e-70 | 219 |
| MsG0880046636.01.T01 | MTR_7g490350 | 37.771 | 323 | 158 | 7 | 42 | 329 | 53 | 367 | 4.09e-69 | 220 |
| MsG0880046636.01.T01 | MTR_5g091980 | 38.344 | 326 | 185 | 6 | 7 | 330 | 11 | 322 | 7.85e-68 | 215 |
| MsG0880046636.01.T01 | MTR_7g090320 | 38.596 | 342 | 162 | 8 | 32 | 329 | 48 | 385 | 1.44e-67 | 217 |
| MsG0880046636.01.T01 | MTR_7g090300 | 35.958 | 381 | 174 | 10 | 6 | 330 | 2 | 368 | 3.07e-66 | 213 |
| MsG0880046636.01.T01 | MTR_2g087780 | 36.550 | 342 | 168 | 8 | 29 | 329 | 60 | 393 | 8.46e-64 | 207 |
| MsG0880046636.01.T01 | MTR_7g090310 | 37.160 | 331 | 161 | 7 | 42 | 329 | 58 | 384 | 6.15e-62 | 202 |
| MsG0880046636.01.T01 | MTR_4g050190 | 35.103 | 339 | 170 | 8 | 32 | 329 | 60 | 389 | 5.51e-61 | 200 |
| MsG0880046636.01.T01 | MTR_2g099030 | 33.036 | 336 | 180 | 6 | 30 | 325 | 70 | 400 | 1.37e-54 | 184 |
| MsG0880046636.01.T01 | MTR_4g112840 | 67.327 | 101 | 30 | 1 | 176 | 276 | 56 | 153 | 2.81e-39 | 137 |
| MsG0880046636.01.T01 | MTR_5g077620 | 72.619 | 84 | 21 | 2 | 8 | 91 | 1 | 82 | 3.08e-34 | 123 |
| MsG0880046636.01.T01 | MTR_8g074210 | 39.181 | 171 | 98 | 4 | 103 | 273 | 118 | 282 | 3.30e-27 | 110 |
| MsG0880046636.01.T01 | MTR_8g074170 | 38.596 | 171 | 99 | 4 | 103 | 273 | 118 | 282 | 1.76e-25 | 105 |
| MsG0880046636.01.T01 | MTR_8g074270 | 38.012 | 171 | 100 | 4 | 103 | 273 | 118 | 282 | 2.95e-25 | 104 |
| MsG0880046636.01.T01 | MTR_2g042430 | 31.381 | 239 | 134 | 8 | 40 | 277 | 74 | 283 | 3.70e-24 | 101 |
| MsG0880046636.01.T01 | MTR_2g058430 | 50.485 | 103 | 42 | 1 | 79 | 181 | 43 | 136 | 2.43e-23 | 97.4 |
| MsG0880046636.01.T01 | MTR_2g042370 | 33.133 | 166 | 105 | 4 | 113 | 277 | 112 | 272 | 1.62e-20 | 91.3 |
| MsG0880046636.01.T01 | MTR_2g042420 | 28.033 | 239 | 142 | 8 | 40 | 277 | 55 | 264 | 7.11e-20 | 89.4 |
| MsG0880046636.01.T01 | MTR_8g074320 | 38.462 | 130 | 72 | 4 | 103 | 229 | 95 | 219 | 2.36e-18 | 82.8 |
| MsG0880046636.01.T01 | MTR_1g075865 | 40.541 | 111 | 62 | 2 | 153 | 262 | 4 | 111 | 2.95e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046636.01.T01 | AT1G74020 | 43.974 | 307 | 156 | 8 | 31 | 329 | 27 | 325 | 1.98e-71 | 225 |
| MsG0880046636.01.T01 | AT3G57030 | 40.187 | 321 | 147 | 6 | 42 | 325 | 56 | 368 | 1.12e-70 | 224 |
| MsG0880046636.01.T01 | AT1G74010 | 43.421 | 304 | 155 | 9 | 31 | 326 | 26 | 320 | 3.82e-69 | 219 |
| MsG0880046636.01.T01 | AT1G74000 | 41.311 | 305 | 167 | 7 | 31 | 329 | 29 | 327 | 1.28e-66 | 213 |
| MsG0880046636.01.T01 | AT3G57010 | 38.235 | 272 | 152 | 4 | 11 | 275 | 16 | 278 | 2.42e-63 | 206 |
| MsG0880046636.01.T01 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.49e-63 | 205 |
| MsG0880046636.01.T01 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.49e-63 | 205 |
| MsG0880046636.01.T01 | AT5G22020 | 37.195 | 328 | 161 | 9 | 40 | 329 | 73 | 393 | 7.49e-63 | 205 |
| MsG0880046636.01.T01 | AT1G08470 | 36.391 | 338 | 166 | 9 | 32 | 329 | 59 | 387 | 1.90e-62 | 204 |
| MsG0880046636.01.T01 | AT3G57020 | 40.000 | 240 | 135 | 2 | 36 | 275 | 47 | 277 | 1.72e-59 | 195 |
| MsG0880046636.01.T01 | AT2G41290 | 36.446 | 332 | 159 | 11 | 42 | 329 | 51 | 374 | 1.17e-58 | 193 |
| MsG0880046636.01.T01 | AT2G41300 | 39.301 | 229 | 130 | 3 | 47 | 275 | 83 | 302 | 7.74e-54 | 181 |
| MsG0880046636.01.T01 | AT3G57020 | 37.917 | 240 | 126 | 2 | 36 | 275 | 47 | 263 | 8.03e-54 | 180 |
| MsG0880046636.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.31e-53 | 179 |
| MsG0880046636.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.31e-53 | 179 |
| MsG0880046636.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 6.31e-53 | 179 |
| MsG0880046636.01.T01 | AT3G51440 | 27.586 | 290 | 177 | 8 | 20 | 301 | 40 | 304 | 6.55e-23 | 98.2 |
| MsG0880046636.01.T01 | AT3G51430 | 35.028 | 177 | 106 | 5 | 103 | 278 | 113 | 281 | 2.38e-21 | 94.0 |
| MsG0880046636.01.T01 | AT3G51430 | 35.057 | 174 | 104 | 5 | 103 | 275 | 113 | 278 | 1.04e-20 | 92.0 |
| MsG0880046636.01.T01 | AT3G51450 | 30.732 | 205 | 125 | 6 | 103 | 302 | 113 | 305 | 5.73e-20 | 90.1 |
| MsG0880046636.01.T01 | AT3G51420 | 29.353 | 201 | 133 | 5 | 103 | 302 | 113 | 305 | 8.20e-20 | 89.4 |
Find 87 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATTCAAGAGGCCAATTT+TGG | 0.175988 | 8:+75984537 | MsG0880046636.01.T01:CDS |
| TATGCAGCTGATGCTTATTA+TGG | 0.243078 | 8:+75983711 | MsG0880046636.01.T01:CDS |
| GAATTCCTCTTTATGTTATC+TGG | 0.262678 | 8:-75984521 | None:intergenic |
| CTTGTGCAGGTCCAACTAAT+TGG | 0.268148 | 8:-75983770 | None:intergenic |
| TATTAACTGCAATCCAAAAT+TGG | 0.282710 | 8:-75984550 | None:intergenic |
| AAATCTGAGGAATTTGCTTT+TGG | 0.297207 | 8:-75984476 | None:intergenic |
| GTTGAACATTAATAATTCTA+TGG | 0.304772 | 8:+75983226 | MsG0880046636.01.T01:CDS |
| TTGGGTTGCTTTCCCTCCAT+TGG | 0.315305 | 8:-75983751 | None:intergenic |
| AAATAAACGATTCCAGTGTT+TGG | 0.321439 | 8:-75983831 | None:intergenic |
| TGGGTTGCTTTCCCTCCATT+GGG | 0.331282 | 8:-75983750 | None:intergenic |
| CTGAAGCTTGTTCAGTAATA+TGG | 0.333503 | 8:-75983306 | None:intergenic |
| AGAAATCTTAAATTTGCATC+TGG | 0.338821 | 8:+75984371 | MsG0880046636.01.T01:CDS |
| TAATAGTCTTACTTTGTCTT+CGG | 0.340893 | 8:+75984568 | MsG0880046636.01.T01:CDS |
| GTCTCTCTTGTTGAAGAGTA+TGG | 0.345465 | 8:+75984629 | MsG0880046636.01.T01:CDS |
| CTGTCAAATGCAAGAGAATC+AGG | 0.348024 | 8:-75983350 | None:intergenic |
| AATAGTCTTACTTTGTCTTC+GGG | 0.365443 | 8:+75984569 | MsG0880046636.01.T01:CDS |
| GATTTATTCATGCTACTTGC+TGG | 0.370329 | 8:+75984494 | MsG0880046636.01.T01:CDS |
| CTGTAAAACTATACCATTCT+CGG | 0.373836 | 8:-75984603 | None:intergenic |
| ATGCAGCTGATGCTTATTAT+GGG | 0.375296 | 8:+75983712 | MsG0880046636.01.T01:CDS |
| ATCAGCAAACACGGTAGAAT+TGG | 0.379198 | 8:-75983793 | None:intergenic |
| AATAAACGATTCCAGTGTTT+GGG | 0.380124 | 8:-75983830 | None:intergenic |
| GCAATATGTGGAAGGCCATT+AGG | 0.400762 | 8:+75983657 | MsG0880046636.01.T01:CDS |
| CACTGGTTAGCAGCGCTGTC+TGG | 0.406496 | 8:-75984280 | None:intergenic |
| TGCTTGGATCATAACTCAAT+AGG | 0.410469 | 8:-75984325 | None:intergenic |
| ACTGTTGTCTGGTTGTTGCT+TGG | 0.420207 | 8:-75984341 | None:intergenic |
| GATTTATATATTTACCTCTT+GGG | 0.420261 | 8:-75983467 | None:intergenic |
| TTGTGCAGGTCCAACTAATT+GGG | 0.425364 | 8:-75983769 | None:intergenic |
| TTCTCAGTAACACTGTTGTC+TGG | 0.431970 | 8:-75984352 | None:intergenic |
| GAGTACAATGGAACACTCTA+TGG | 0.432828 | 8:+75984680 | MsG0880046636.01.T01:CDS |
| TGTGGAAGGCCATTAGGATT+GGG | 0.435505 | 8:+75983663 | MsG0880046636.01.T01:CDS |
| TTGGGTTTCAATCATCAAAC+AGG | 0.444949 | 8:+75983681 | MsG0880046636.01.T01:CDS |
| AGAGTATGGTCTTCTTGAAC+CGG | 0.453051 | 8:+75984643 | MsG0880046636.01.T01:CDS |
| ATGGCCTTCCACATATTGCT+TGG | 0.453479 | 8:-75983653 | None:intergenic |
| CTTGTAAAGGTTGGCCCCAA+TGG | 0.461054 | 8:+75983735 | MsG0880046636.01.T01:CDS |
| ATGTGGAAGGCCATTAGGAT+TGG | 0.461713 | 8:+75983662 | MsG0880046636.01.T01:CDS |
| AAAACTATGCTTACTTGAGC+TGG | 0.464925 | 8:-75983875 | None:intergenic |
| GAAATCTTAAATTTGCATCT+GGG | 0.465666 | 8:+75984372 | MsG0880046636.01.T01:CDS |
| TGGTCCAAGTGAAGGTTTCA+AGG | 0.466762 | 8:+75983427 | MsG0880046636.01.T01:CDS |
| TATTATGGGCTTGTAAAGGT+TGG | 0.470412 | 8:+75983726 | MsG0880046636.01.T01:CDS |
| CAGTAGAAAGATAGCAAGAA+TGG | 0.470940 | 8:-75983264 | None:intergenic |
| AGTGGCATTGGAGATAATTC+AGG | 0.481306 | 8:+75984299 | MsG0880046636.01.T01:CDS |
| TACTCTTGAACCTCACTAGC+CGG | 0.499543 | 8:-75984662 | None:intergenic |
| GGGAAAGCAACCCAATTAGT+TGG | 0.500291 | 8:+75983759 | MsG0880046636.01.T01:CDS |
| GCGCTGCTAACCAGTGGCAT+TGG | 0.502363 | 8:+75984287 | MsG0880046636.01.T01:CDS |
| TGATTGAAACCCAATCCTAA+TGG | 0.505270 | 8:-75983672 | None:intergenic |
| TCTCTTGCATTTGACAGAAA+CGG | 0.511849 | 8:+75983356 | MsG0880046636.01.T01:CDS |
| AATTCTACCGTGTTTGCTGA+TGG | 0.517101 | 8:+75983795 | MsG0880046636.01.T01:CDS |
| TGAATTATCTCCAATGCCAC+TGG | 0.518510 | 8:-75984297 | None:intergenic |
| CTTGTTCAGTAATATGGCTA+TGG | 0.523436 | 8:-75983300 | None:intergenic |
| CCATCAGAAGAAGTAACATA+AGG | 0.525792 | 8:-75983386 | None:intergenic |
| CCTTATGTTACTTCTTCTGA+TGG | 0.527626 | 8:+75983386 | MsG0880046636.01.T01:CDS |
| AAATATGTTGGTCCAAGTGA+AGG | 0.529246 | 8:+75983419 | MsG0880046636.01.T01:CDS |
| GGTGTTAGAGTGACCGAGAA+TGG | 0.530351 | 8:+75984590 | MsG0880046636.01.T01:CDS |
| TCAGAAATCCAAGCAATATG+TGG | 0.532707 | 8:+75983645 | MsG0880046636.01.T01:CDS |
| TGCTTATTATGGGCTTGTAA+AGG | 0.537666 | 8:+75983722 | MsG0880046636.01.T01:CDS |
| GGGGTGGCAGTAAGCGGAGA+TGG | 0.543707 | 8:+75984392 | MsG0880046636.01.T01:CDS |
| ACGGTAGAATTGGCTTGTGC+AGG | 0.544655 | 8:-75983783 | None:intergenic |
| ATTCGGAGAGTGTGGTTGAA+AGG | 0.547509 | 8:+75984452 | MsG0880046636.01.T01:CDS |
| TGATTGCATAAGAAGCAAGC+AGG | 0.550700 | 8:-75984709 | None:intergenic |
| GAAGACCAGATAACATAAAG+AGG | 0.552163 | 8:+75984516 | MsG0880046636.01.T01:CDS |
| AGAGAATCAGGTCCTTTCAC+TGG | 0.553043 | 8:-75983338 | None:intergenic |
| TTAGATGTGGACCCAAACAC+TGG | 0.559091 | 8:+75983819 | MsG0880046636.01.T01:CDS |
| AAATCTTAAATTTGCATCTG+GGG | 0.560132 | 8:+75984373 | MsG0880046636.01.T01:CDS |
| TCAGGTCCTTTCACTGGTGA+TGG | 0.564770 | 8:-75983332 | None:intergenic |
| GTAAAGGTTGGCCCCAATGG+AGG | 0.566207 | 8:+75983738 | MsG0880046636.01.T01:CDS |
| TAAAGGTTGGCCCCAATGGA+GGG | 0.566544 | 8:+75983739 | MsG0880046636.01.T01:CDS |
| TATTCCTTGAAACCTTCACT+TGG | 0.569604 | 8:-75983431 | None:intergenic |
| CATTAATAATTCTATGGTGA+TGG | 0.573040 | 8:+75983232 | MsG0880046636.01.T01:CDS |
| TCTTCTTGAACCGGCTAGTG+AGG | 0.578667 | 8:+75984652 | MsG0880046636.01.T01:CDS |
| CTAACACAATTCGGAGAGTG+TGG | 0.584118 | 8:+75984444 | MsG0880046636.01.T01:CDS |
| GTTTGCTGATGGTTTAGATG+TGG | 0.585669 | 8:+75983806 | MsG0880046636.01.T01:CDS |
| GGGTTGCTTTCCCTCCATTG+GGG | 0.591391 | 8:-75983749 | None:intergenic |
| CTGGAATTTAGTGCTAGCTA+TGG | 0.599531 | 8:-75983856 | None:intergenic |
| AGGAACAGGACAATCTGTGA+TGG | 0.601201 | 8:+75983609 | MsG0880046636.01.T01:intron |
| ATCTAAACCATCAGCAAACA+CGG | 0.601997 | 8:-75983802 | None:intergenic |
| GCATCTGGGGTGGCAGTAAG+CGG | 0.604290 | 8:+75984386 | MsG0880046636.01.T01:CDS |
| TCTTAAATTTGCATCTGGGG+TGG | 0.610328 | 8:+75984376 | MsG0880046636.01.T01:CDS |
| AAATCCAAGCAATATGTGGA+AGG | 0.615490 | 8:+75983649 | MsG0880046636.01.T01:CDS |
| GCATTTGACAGAAACGGTGA+AGG | 0.616080 | 8:+75983362 | MsG0880046636.01.T01:CDS |
| CAGCTACCATCACCAGTGAA+AGG | 0.618564 | 8:+75983326 | MsG0880046636.01.T01:CDS |
| AACATAAAGAGGAATTCAAG+AGG | 0.628213 | 8:+75984527 | MsG0880046636.01.T01:CDS |
| GATTGCATAAGAAGCAAGCA+GGG | 0.638280 | 8:-75984708 | None:intergenic |
| TACAATGGAACACTCTATGG+TGG | 0.656288 | 8:+75984683 | MsG0880046636.01.T01:CDS |
| AAGTAGCATGAATAAATCTG+AGG | 0.664600 | 8:-75984489 | None:intergenic |
| CAGACAGCGCTGCTAACCAG+TGG | 0.665038 | 8:+75984281 | MsG0880046636.01.T01:CDS |
| ATGCTTACACTTCTCCCAAG+AGG | 0.667804 | 8:+75983453 | MsG0880046636.01.T01:CDS |
| AGTGAGGTTCAAGAGTACAA+TGG | 0.673039 | 8:+75984668 | MsG0880046636.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAATTACTATGTAAA+TGG | - | Chr8:75983528-75983547 | None:intergenic | 10.0% |
| !!! | AATGATTGTTTTTTATTTTT+TGG | + | Chr8:75983583-75983602 | MsG0880046636.01.T01:intron | 10.0% |
| !! | TGATAATTTATGAGAAAAAT+TGG | - | Chr8:75983934-75983953 | None:intergenic | 15.0% |
| !! | TTCAATTATGTTATTGTTTA+TGG | + | Chr8:75984150-75984169 | MsG0880046636.01.T01:intron | 15.0% |
| !! | GATTTATATATTTACCTCTT+GGG | - | Chr8:75983470-75983489 | None:intergenic | 20.0% |
| !! | GTTGAACATTAATAATTCTA+TGG | + | Chr8:75983226-75983245 | MsG0880046636.01.T01:CDS | 20.0% |
| !! | TGATTTATATATTTACCTCT+TGG | - | Chr8:75983471-75983490 | None:intergenic | 20.0% |
| !!! | AATATTTTGCTAACACAATT+CGG | + | Chr8:75984435-75984454 | MsG0880046636.01.T01:CDS | 20.0% |
| !!! | TGTTTTTTATTTTTTGGTTC+AGG | + | Chr8:75983589-75983608 | MsG0880046636.01.T01:intron | 20.0% |
| ! | AAATCTTAAATTTGCATCTG+GGG | + | Chr8:75984373-75984392 | MsG0880046636.01.T01:CDS | 25.0% |
| ! | AGAAATCTTAAATTTGCATC+TGG | + | Chr8:75984371-75984390 | MsG0880046636.01.T01:CDS | 25.0% |
| ! | CATTAATAATTCTATGGTGA+TGG | + | Chr8:75983232-75983251 | MsG0880046636.01.T01:CDS | 25.0% |
| ! | CTTTCTTTCCTCAAATAATT+TGG | + | Chr8:75984123-75984142 | MsG0880046636.01.T01:intron | 25.0% |
| ! | GAAATCTTAAATTTGCATCT+GGG | + | Chr8:75984372-75984391 | MsG0880046636.01.T01:CDS | 25.0% |
| ! | TATTAACTGCAATCCAAAAT+TGG | - | Chr8:75984553-75984572 | None:intergenic | 25.0% |
| !! | GGAAGAATTTTCAAATATGT+TGG | + | Chr8:75983407-75983426 | MsG0880046636.01.T01:CDS | 25.0% |
| !! | GTTTAGTTTCAAAATCATAG+AGG | + | Chr8:75984187-75984206 | MsG0880046636.01.T01:intron | 25.0% |
| !! | TAATAGTCTTACTTTGTCTT+CGG | + | Chr8:75984568-75984587 | MsG0880046636.01.T01:CDS | 25.0% |
| AAATAAACGATTCCAGTGTT+TGG | - | Chr8:75983834-75983853 | None:intergenic | 30.0% | |
| AACATAAAGAGGAATTCAAG+AGG | + | Chr8:75984527-75984546 | MsG0880046636.01.T01:CDS | 30.0% | |
| AAGTAGCATGAATAAATCTG+AGG | - | Chr8:75984492-75984511 | None:intergenic | 30.0% | |
| AATAAACGATTCCAGTGTTT+GGG | - | Chr8:75983833-75983852 | None:intergenic | 30.0% | |
| CTGTAAAACTATACCATTCT+CGG | - | Chr8:75984606-75984625 | None:intergenic | 30.0% | |
| CTTTCCTCAAATAATTTGGA+GGG | + | Chr8:75984127-75984146 | MsG0880046636.01.T01:intron | 30.0% | |
| GAATTCCTCTTTATGTTATC+TGG | - | Chr8:75984524-75984543 | None:intergenic | 30.0% | |
| TCTTTCCTCAAATAATTTGG+AGG | + | Chr8:75984126-75984145 | MsG0880046636.01.T01:intron | 30.0% | |
| ! | AATAGTCTTACTTTGTCTTC+GGG | + | Chr8:75984569-75984588 | MsG0880046636.01.T01:CDS | 30.0% |
| !! | AAATCTGAGGAATTTGCTTT+TGG | - | Chr8:75984479-75984498 | None:intergenic | 30.0% |
| !!! | TTATTTTTTGGTTCAGGAAC+AGG | + | Chr8:75983595-75983614 | MsG0880046636.01.T01:intron | 30.0% |
| AAAACTATGCTTACTTGAGC+TGG | - | Chr8:75983878-75983897 | None:intergenic | 35.0% | |
| AAATATGTTGGTCCAAGTGA+AGG | + | Chr8:75983419-75983438 | MsG0880046636.01.T01:CDS | 35.0% | |
| AAATCCAAGCAATATGTGGA+AGG | + | Chr8:75983649-75983668 | MsG0880046636.01.T01:CDS | 35.0% | |
| ATCTAAACCATCAGCAAACA+CGG | - | Chr8:75983805-75983824 | None:intergenic | 35.0% | |
| ATGCAGCTGATGCTTATTAT+GGG | + | Chr8:75983712-75983731 | MsG0880046636.01.T01:CDS | 35.0% | |
| ATGGTCACTTGATTCATGTT+AGG | - | Chr8:75983509-75983528 | None:intergenic | 35.0% | |
| CAGTAGAAAGATAGCAAGAA+TGG | - | Chr8:75983267-75983286 | None:intergenic | 35.0% | |
| CCATCAGAAGAAGTAACATA+AGG | - | Chr8:75983389-75983408 | None:intergenic | 35.0% | |
| CTTGTTCAGTAATATGGCTA+TGG | - | Chr8:75983303-75983322 | None:intergenic | 35.0% | |
| GAAGACCAGATAACATAAAG+AGG | + | Chr8:75984516-75984535 | MsG0880046636.01.T01:CDS | 35.0% | |
| TATGCAGCTGATGCTTATTA+TGG | + | Chr8:75983711-75983730 | MsG0880046636.01.T01:CDS | 35.0% | |
| TATTATGGGCTTGTAAAGGT+TGG | + | Chr8:75983726-75983745 | MsG0880046636.01.T01:CDS | 35.0% | |
| TATTCCTTGAAACCTTCACT+TGG | - | Chr8:75983434-75983453 | None:intergenic | 35.0% | |
| TCAGAAATCCAAGCAATATG+TGG | + | Chr8:75983645-75983664 | MsG0880046636.01.T01:CDS | 35.0% | |
| TCTCTTGCATTTGACAGAAA+CGG | + | Chr8:75983356-75983375 | MsG0880046636.01.T01:CDS | 35.0% | |
| TGAACCCTCCAAATTATTTG+AGG | - | Chr8:75984134-75984153 | None:intergenic | 35.0% | |
| TGATTGAAACCCAATCCTAA+TGG | - | Chr8:75983675-75983694 | None:intergenic | 35.0% | |
| TGCTTATTATGGGCTTGTAA+AGG | + | Chr8:75983722-75983741 | MsG0880046636.01.T01:CDS | 35.0% | |
| TGCTTGGATCATAACTCAAT+AGG | - | Chr8:75984328-75984347 | None:intergenic | 35.0% | |
| TGGTCACTTGATTCATGTTA+GGG | - | Chr8:75983508-75983527 | None:intergenic | 35.0% | |
| TTGGGTTTCAATCATCAAAC+AGG | + | Chr8:75983681-75983700 | MsG0880046636.01.T01:CDS | 35.0% | |
| ! | CCTTATGTTACTTCTTCTGA+TGG | + | Chr8:75983386-75983405 | MsG0880046636.01.T01:CDS | 35.0% |
| ! | CTGAAGCTTGTTCAGTAATA+TGG | - | Chr8:75983309-75983328 | None:intergenic | 35.0% |
| ! | GATTTATTCATGCTACTTGC+TGG | + | Chr8:75984494-75984513 | MsG0880046636.01.T01:CDS | 35.0% |
| AGAGTATGGTCTTCTTGAAC+CGG | + | Chr8:75984643-75984662 | MsG0880046636.01.T01:CDS | 40.0% | |
| ATCAGCAAACACGGTAGAAT+TGG | - | Chr8:75983796-75983815 | None:intergenic | 40.0% | |
| CTGGAATTTAGTGCTAGCTA+TGG | - | Chr8:75983859-75983878 | None:intergenic | 40.0% | |
| CTGTCAAATGCAAGAGAATC+AGG | - | Chr8:75983353-75983372 | None:intergenic | 40.0% | |
| GAGTACAATGGAACACTCTA+TGG | + | Chr8:75984680-75984699 | MsG0880046636.01.T01:CDS | 40.0% | |
| GATTGCATAAGAAGCAAGCA+GGG | - | Chr8:75984711-75984730 | None:intergenic | 40.0% | |
| GTCTCTCTTGTTGAAGAGTA+TGG | + | Chr8:75984629-75984648 | MsG0880046636.01.T01:CDS | 40.0% | |
| TACAATGGAACACTCTATGG+TGG | + | Chr8:75984683-75984702 | MsG0880046636.01.T01:CDS | 40.0% | |
| TCTTAAATTTGCATCTGGGG+TGG | + | Chr8:75984376-75984395 | MsG0880046636.01.T01:CDS | 40.0% | |
| TGAATTATCTCCAATGCCAC+TGG | - | Chr8:75984300-75984319 | None:intergenic | 40.0% | |
| TGATTGCATAAGAAGCAAGC+AGG | - | Chr8:75984712-75984731 | None:intergenic | 40.0% | |
| TTCTCAGTAACACTGTTGTC+TGG | - | Chr8:75984355-75984374 | None:intergenic | 40.0% | |
| TTGTGCAGGTCCAACTAATT+GGG | - | Chr8:75983772-75983791 | None:intergenic | 40.0% | |
| ! | AATTCTACCGTGTTTGCTGA+TGG | + | Chr8:75983795-75983814 | MsG0880046636.01.T01:CDS | 40.0% |
| ! | AGTGAGGTTCAAGAGTACAA+TGG | + | Chr8:75984668-75984687 | MsG0880046636.01.T01:CDS | 40.0% |
| ! | AGTGGCATTGGAGATAATTC+AGG | + | Chr8:75984299-75984318 | MsG0880046636.01.T01:CDS | 40.0% |
| ! | GGAATTCAAGAGGCCAATTT+TGG | + | Chr8:75984537-75984556 | MsG0880046636.01.T01:CDS | 40.0% |
| ! | GTTTGCTGATGGTTTAGATG+TGG | + | Chr8:75983806-75983825 | MsG0880046636.01.T01:CDS | 40.0% |
| ACTGTTGTCTGGTTGTTGCT+TGG | - | Chr8:75984344-75984363 | None:intergenic | 45.0% | |
| AGAGAATCAGGTCCTTTCAC+TGG | - | Chr8:75983341-75983360 | None:intergenic | 45.0% | |
| AGCATGCGTCACAGATGTTA+GGG | - | Chr8:75984089-75984108 | None:intergenic | 45.0% | |
| AGGAACAGGACAATCTGTGA+TGG | + | Chr8:75983609-75983628 | MsG0880046636.01.T01:intron | 45.0% | |
| ATGCTTACACTTCTCCCAAG+AGG | + | Chr8:75983453-75983472 | MsG0880046636.01.T01:CDS | 45.0% | |
| ATGGCCTTCCACATATTGCT+TGG | - | Chr8:75983656-75983675 | None:intergenic | 45.0% | |
| ATGTGGAAGGCCATTAGGAT+TGG | + | Chr8:75983662-75983681 | MsG0880046636.01.T01:CDS | 45.0% | |
| CTAACACAATTCGGAGAGTG+TGG | + | Chr8:75984444-75984463 | MsG0880046636.01.T01:CDS | 45.0% | |
| CTTGTGCAGGTCCAACTAAT+TGG | - | Chr8:75983773-75983792 | None:intergenic | 45.0% | |
| GCAATATGTGGAAGGCCATT+AGG | + | Chr8:75983657-75983676 | MsG0880046636.01.T01:CDS | 45.0% | |
| GCATTTGACAGAAACGGTGA+AGG | + | Chr8:75983362-75983381 | MsG0880046636.01.T01:CDS | 45.0% | |
| GGGAAAGCAACCCAATTAGT+TGG | + | Chr8:75983759-75983778 | MsG0880046636.01.T01:CDS | 45.0% | |
| TACTCTTGAACCTCACTAGC+CGG | - | Chr8:75984665-75984684 | None:intergenic | 45.0% | |
| TGGTCCAAGTGAAGGTTTCA+AGG | + | Chr8:75983427-75983446 | MsG0880046636.01.T01:CDS | 45.0% | |
| TGTGGAAGGCCATTAGGATT+GGG | + | Chr8:75983663-75983682 | MsG0880046636.01.T01:CDS | 45.0% | |
| TTAGATGTGGACCCAAACAC+TGG | + | Chr8:75983819-75983838 | MsG0880046636.01.T01:CDS | 45.0% | |
| ! | ATTCGGAGAGTGTGGTTGAA+AGG | + | Chr8:75984452-75984471 | MsG0880046636.01.T01:CDS | 45.0% |
| CAGCTACCATCACCAGTGAA+AGG | + | Chr8:75983326-75983345 | MsG0880046636.01.T01:CDS | 50.0% | |
| GAGCATGCGTCACAGATGTT+AGG | - | Chr8:75984090-75984109 | None:intergenic | 50.0% | |
| GCATGCGTCACAGATGTTAG+GGG | - | Chr8:75984088-75984107 | None:intergenic | 50.0% | |
| GGTGTTAGAGTGACCGAGAA+TGG | + | Chr8:75984590-75984609 | MsG0880046636.01.T01:CDS | 50.0% | |
| TCAGGTCCTTTCACTGGTGA+TGG | - | Chr8:75983335-75983354 | None:intergenic | 50.0% | |
| TCTTCTTGAACCGGCTAGTG+AGG | + | Chr8:75984652-75984671 | MsG0880046636.01.T01:CDS | 50.0% | |
| ! | CTTGTAAAGGTTGGCCCCAA+TGG | + | Chr8:75983735-75983754 | MsG0880046636.01.T01:CDS | 50.0% |
| ! | TAAAGGTTGGCCCCAATGGA+GGG | + | Chr8:75983739-75983758 | MsG0880046636.01.T01:CDS | 50.0% |
| ! | TGGGTTGCTTTCCCTCCATT+GGG | - | Chr8:75983753-75983772 | None:intergenic | 50.0% |
| ! | TTGGGTTGCTTTCCCTCCAT+TGG | - | Chr8:75983754-75983773 | None:intergenic | 50.0% |
| !! | ACGGTAGAATTGGCTTGTGC+AGG | - | Chr8:75983786-75983805 | None:intergenic | 50.0% |
| ! | GGGTTGCTTTCCCTCCATTG+GGG | - | Chr8:75983752-75983771 | None:intergenic | 55.0% |
| ! | GTAAAGGTTGGCCCCAATGG+AGG | + | Chr8:75983738-75983757 | MsG0880046636.01.T01:CDS | 55.0% |
| CAGACAGCGCTGCTAACCAG+TGG | + | Chr8:75984281-75984300 | MsG0880046636.01.T01:CDS | 60.0% | |
| GCGCTGCTAACCAGTGGCAT+TGG | + | Chr8:75984287-75984306 | MsG0880046636.01.T01:CDS | 60.0% | |
| ! | CACTGGTTAGCAGCGCTGTC+TGG | - | Chr8:75984283-75984302 | None:intergenic | 60.0% |
| ! | GCATCTGGGGTGGCAGTAAG+CGG | + | Chr8:75984386-75984405 | MsG0880046636.01.T01:CDS | 60.0% |
| GGGGTGGCAGTAAGCGGAGA+TGG | + | Chr8:75984392-75984411 | MsG0880046636.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 75983224 | 75984745 | 75983224 | ID=MsG0880046636.01;Name=MsG0880046636.01 |
| Chr8 | mRNA | 75983224 | 75984745 | 75983224 | ID=MsG0880046636.01.T01;Parent=MsG0880046636.01;Name=MsG0880046636.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|331 |
| Chr8 | exon | 75983224 | 75983474 | 75983224 | ID=MsG0880046636.01.T01:exon:32394;Parent=MsG0880046636.01.T01 |
| Chr8 | exon | 75983611 | 75983883 | 75983611 | ID=MsG0880046636.01.T01:exon:32395;Parent=MsG0880046636.01.T01 |
| Chr8 | exon | 75984274 | 75984745 | 75984274 | ID=MsG0880046636.01.T01:exon:32396;Parent=MsG0880046636.01.T01 |
| Chr8 | CDS | 75983224 | 75983474 | 75983224 | ID=MsG0880046636.01.T01:cds;Parent=MsG0880046636.01.T01 |
| Chr8 | CDS | 75983611 | 75983883 | 75983611 | ID=MsG0880046636.01.T01:cds;Parent=MsG0880046636.01.T01 |
| Chr8 | CDS | 75984274 | 75984745 | 75984274 | ID=MsG0880046636.01.T01:cds;Parent=MsG0880046636.01.T01 |
| Gene Sequence |
| Protein sequence |
