AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000019.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000019.01.T01 MTR_1g026110 91.732 254 14 2 1 254 1 247 2.53e-163 452
MsG0180000019.01.T01 MTR_3g114750 48.485 264 98 11 4 250 2 244 3.17e-59 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000019.01.T01 AT1G76070 33.212 274 120 10 15 250 24 272 2.87e-28 108
MsG0180000019.01.T01 AT1G20310 34.686 271 120 11 1 247 1 238 3.60e-27 105

Find 61 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGATCTTTGTTGGGTTT+TGG 0.236347 1:+399997 None:intergenic
TCCCTTTGATCAATTTCTTC+AGG 0.304197 1:+399751 None:intergenic
TTCACCAAAGATTTCTTGTA+TGG 0.307383 1:-400052 MsG0180000019.01.T01:CDS
TCATTGCCACCACCATCTTT+AGG 0.317528 1:+400090 None:intergenic
CCTAAAATAGGTGCAGAAAA+TGG 0.326900 1:+399691 None:intergenic
TTTCCACCAACACCTAAAAT+AGG 0.335034 1:+399679 None:intergenic
CTAAATTTGTGATCTCTTCC+TGG 0.339147 1:+400216 None:intergenic
TTTACCTCCGTATCCACCTT+TGG 0.375428 1:+399955 None:intergenic
GAAAGGAAATCAGATGGTTC+TGG 0.386986 1:-399880 MsG0180000019.01.T01:CDS
CAAGAAATCTTTGGTGAAGT+AGG 0.393822 1:+400057 None:intergenic
CAAGGAATGAGATTAATCTT+TGG 0.407911 1:-399618 MsG0180000019.01.T01:CDS
TTAGGTGTTGGTGGAAATAG+TGG 0.423953 1:-399673 MsG0180000019.01.T01:CDS
TGTCCCATACAAGAAATCTT+TGG 0.426848 1:+400048 None:intergenic
GCCTGAAGAAATTGATCAAA+GGG 0.432040 1:-399752 MsG0180000019.01.T01:CDS
GGAAGTGAAGAGATCTTTGT+TGG 0.442034 1:+399990 None:intergenic
CAAAGATTAATCTCATTCCT+TGG 0.442885 1:+399619 None:intergenic
CAAAACGCTCCTTTCAGTCC+AGG 0.469371 1:-400234 MsG0180000019.01.T01:CDS
AGAAAATAACACAAAGTCAT+GGG 0.481776 1:-400190 MsG0180000019.01.T01:CDS
GGTTCTGGATTTGTTGATGA+AGG 0.482613 1:-399865 MsG0180000019.01.T01:CDS
CACTCCACCAAAGGTGGATA+CGG 0.483600 1:-399962 MsG0180000019.01.T01:CDS
CAAAGGGATTGCTTTACTGA+TGG 0.491176 1:-399736 MsG0180000019.01.T01:CDS
ACCTCCGTATCCACCTTTGG+TGG 0.491667 1:+399958 None:intergenic
CACCGCCGATGGGTGATATG+AGG 0.497283 1:-399831 MsG0180000019.01.T01:CDS
GGATTCAACTGAAGAGGCCT+AGG 0.502469 1:+399574 None:intergenic
ATTTATTTAGCTTTAAGCAC+AGG 0.503675 1:+399553 None:intergenic
AAAACTCATGCTACTGGTAA+GGG 0.508213 1:-400162 MsG0180000019.01.T01:CDS
CGTATCCACCTTTGGTGGAG+TGG 0.514538 1:+399963 None:intergenic
CTGATTTGAAGTTTAAGCCA+AGG 0.518893 1:-399636 MsG0180000019.01.T01:CDS
TGATCTCTTCCTGGACTGAA+AGG 0.521071 1:+400225 None:intergenic
CAGAAAATAACACAAAGTCA+TGG 0.527453 1:-400191 MsG0180000019.01.T01:CDS
AAGAGAAACAAAGTAAGCTA+AGG 0.530956 1:-400290 MsG0180000019.01.T01:CDS
GCTAGAAGAAAACCTAAAGA+TGG 0.536495 1:-400102 MsG0180000019.01.T01:CDS
GAAGTGAAGAGATCTTTGTT+GGG 0.537217 1:+399991 None:intergenic
CACCTTTGGTGGAGTGGACA+CGG 0.538901 1:+399969 None:intergenic
AGCCTGAAGAAATTGATCAA+AGG 0.541841 1:-399753 MsG0180000019.01.T01:CDS
TAAAGAGATCGAAGTTTCAG+AGG 0.548970 1:-399936 MsG0180000019.01.T01:CDS
CGTGTCCACTCCACCAAAGG+TGG 0.560517 1:-399968 MsG0180000019.01.T01:CDS
AACAACAAAGCACCGCCGAT+GGG 0.567253 1:-399841 MsG0180000019.01.T01:CDS
AGCACAGGATTCAACTGAAG+AGG 0.568748 1:+399568 None:intergenic
TCACCAAAGATTTCTTGTAT+GGG 0.570550 1:-400051 MsG0180000019.01.T01:CDS
TTCCGTGTCCACTCCACCAA+AGG 0.573551 1:-399971 MsG0180000019.01.T01:CDS
GTTCTGGATTTGTTGATGAA+GGG 0.581024 1:-399864 MsG0180000019.01.T01:CDS
AGAAAACCTAAAGATGGTGG+TGG 0.587690 1:-400096 MsG0180000019.01.T01:CDS
TATGGAATCATAGTAACCAT+AGG 0.589627 1:+400129 None:intergenic
GAACAACAAAGCACCGCCGA+TGG 0.591209 1:-399842 MsG0180000019.01.T01:CDS
GAGATTTGCAAGTGGACGTG+AGG 0.598149 1:-399809 MsG0180000019.01.T01:CDS
TTTGCGGAAAGGAAATCAGA+TGG 0.598533 1:-399886 MsG0180000019.01.T01:CDS
TTGCTTTACTGATGGAGAGG+AGG 0.603818 1:-399728 MsG0180000019.01.T01:CDS
GGATTGCTTTACTGATGGAG+AGG 0.611817 1:-399731 MsG0180000019.01.T01:CDS
CTAAAGCGAAATTTGCGGAA+AGG 0.612185 1:-399897 MsG0180000019.01.T01:CDS
GAAGAACAATGGCTCCACCT+AGG 0.627085 1:-399591 MsG0180000019.01.T01:CDS
TTCAACTGAAGAGGCCTAGG+TGG 0.634353 1:+399577 None:intergenic
AGAAGAAAACCTAAAGATGG+TGG 0.634473 1:-400099 MsG0180000019.01.T01:CDS
TCTTTGGAAGAGAAGAACAA+TGG 0.638654 1:-399602 MsG0180000019.01.T01:CDS
AATCTCCTCATATCACCCAT+CGG 0.643282 1:+399826 None:intergenic
GAAAATAACACAAAGTCATG+GGG 0.645047 1:-400189 MsG0180000019.01.T01:CDS
AAATAGTGGTACATCTAATG+TGG 0.671533 1:-399659 MsG0180000019.01.T01:CDS
TCCACCAAAGGTGGATACGG+AGG 0.678054 1:-399959 MsG0180000019.01.T01:CDS
CTCCTCATATCACCCATCGG+CGG 0.680805 1:+399829 None:intergenic
GAGGTCTAAAGCGAAATTTG+CGG 0.681767 1:-399902 MsG0180000019.01.T01:CDS
GATATGAGGAGATTTGCAAG+TGG 0.723470 1:-399817 MsG0180000019.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTAAATTTGATTGGA+AGG - Chr1:400067-400086 MsG0180000019.01.T01:CDS 20.0%
! AGAAAATAACACAAAGTCAT+GGG - Chr1:399662-399681 MsG0180000019.01.T01:CDS 25.0%
!!! AGGCTTTTTCTAAATTTGAT+TGG - Chr1:400063-400082 MsG0180000019.01.T01:CDS 25.0%
AAATAGTGGTACATCTAATG+TGG - Chr1:400193-400212 MsG0180000019.01.T01:CDS 30.0%
AAGAGAAACAAAGTAAGCTA+AGG - Chr1:399562-399581 MsG0180000019.01.T01:CDS 30.0%
CAAAGATTAATCTCATTCCT+TGG + Chr1:400236-400255 None:intergenic 30.0%
CAAGGAATGAGATTAATCTT+TGG - Chr1:400234-400253 MsG0180000019.01.T01:CDS 30.0%
CAGAAAATAACACAAAGTCA+TGG - Chr1:399661-399680 MsG0180000019.01.T01:CDS 30.0%
GAAAATAACACAAAGTCATG+GGG - Chr1:399663-399682 MsG0180000019.01.T01:CDS 30.0%
TATGGAATCATAGTAACCAT+AGG + Chr1:399726-399745 None:intergenic 30.0%
TCACCAAAGATTTCTTGTAT+GGG - Chr1:399801-399820 MsG0180000019.01.T01:CDS 30.0%
TTCACCAAAGATTTCTTGTA+TGG - Chr1:399800-399819 MsG0180000019.01.T01:CDS 30.0%
AAAAACTCATGCTACTGGTA+AGG - Chr1:399689-399708 MsG0180000019.01.T01:CDS 35.0%
AAAACTCATGCTACTGGTAA+GGG - Chr1:399690-399709 MsG0180000019.01.T01:CDS 35.0%
AGAAGAAAACCTAAAGATGG+TGG - Chr1:399753-399772 MsG0180000019.01.T01:CDS 35.0%
AGCCTGAAGAAATTGATCAA+AGG - Chr1:400099-400118 MsG0180000019.01.T01:CDS 35.0%
CAAGAAATCTTTGGTGAAGT+AGG + Chr1:399798-399817 None:intergenic 35.0%
CCTAAAATAGGTGCAGAAAA+TGG + Chr1:400164-400183 None:intergenic 35.0%
CTAAATTTGTGATCTCTTCC+TGG + Chr1:399639-399658 None:intergenic 35.0%
GAAGTGAAGAGATCTTTGTT+GGG + Chr1:399864-399883 None:intergenic 35.0%
GCCTGAAGAAATTGATCAAA+GGG - Chr1:400100-400119 MsG0180000019.01.T01:CDS 35.0%
GCTAGAAGAAAACCTAAAGA+TGG - Chr1:399750-399769 MsG0180000019.01.T01:CDS 35.0%
TAAAGAGATCGAAGTTTCAG+AGG - Chr1:399916-399935 MsG0180000019.01.T01:CDS 35.0%
TCCCTTTGATCAATTTCTTC+AGG + Chr1:400104-400123 None:intergenic 35.0%
TGTCCCATACAAGAAATCTT+TGG + Chr1:399807-399826 None:intergenic 35.0%
TTTCCACCAACACCTAAAAT+AGG + Chr1:400176-400195 None:intergenic 35.0%
! AAGAGATCTTTGTTGGGTTT+TGG + Chr1:399858-399877 None:intergenic 35.0%
! CCATTTTCTGCACCTATTTT+AGG - Chr1:400161-400180 MsG0180000019.01.T01:CDS 35.0%
! CTGATTTGAAGTTTAAGCCA+AGG - Chr1:400216-400235 MsG0180000019.01.T01:CDS 35.0%
! TCTTTGGAAGAGAAGAACAA+TGG - Chr1:400250-400269 MsG0180000019.01.T01:CDS 35.0%
!! GTTCTGGATTTGTTGATGAA+GGG - Chr1:399988-400007 MsG0180000019.01.T01:CDS 35.0%
!!! GGTTTTCTTCTAGCTTCATA+TGG + Chr1:399744-399763 None:intergenic 35.0%
AATCTCCTCATATCACCCAT+CGG + Chr1:400029-400048 None:intergenic 40.0%
CAAAGGGATTGCTTTACTGA+TGG - Chr1:400116-400135 MsG0180000019.01.T01:CDS 40.0%
CTAAAGCGAAATTTGCGGAA+AGG - Chr1:399955-399974 MsG0180000019.01.T01:CDS 40.0%
GAAAGGAAATCAGATGGTTC+TGG - Chr1:399972-399991 MsG0180000019.01.T01:CDS 40.0%
GATATGAGGAGATTTGCAAG+TGG - Chr1:400035-400054 MsG0180000019.01.T01:CDS 40.0%
GGAAGTGAAGAGATCTTTGT+TGG + Chr1:399865-399884 None:intergenic 40.0%
TTTGCGGAAAGGAAATCAGA+TGG - Chr1:399966-399985 MsG0180000019.01.T01:CDS 40.0%
! AGAAAACCTAAAGATGGTGG+TGG - Chr1:399756-399775 MsG0180000019.01.T01:CDS 40.0%
! GAGGTCTAAAGCGAAATTTG+CGG - Chr1:399950-399969 MsG0180000019.01.T01:CDS 40.0%
! TAAGGGATTTTCTGGACCTA+TGG - Chr1:399707-399726 MsG0180000019.01.T01:CDS 40.0%
!! GGTTCTGGATTTGTTGATGA+AGG - Chr1:399987-400006 MsG0180000019.01.T01:CDS 40.0%
!! TCTGCACCTATTTTAGGTGT+TGG - Chr1:400167-400186 MsG0180000019.01.T01:CDS 40.0%
!! TTAGGTGTTGGTGGAAATAG+TGG - Chr1:400179-400198 MsG0180000019.01.T01:CDS 40.0%
!!! TTCAGAGGATTTTTTCGCAG+AGG - Chr1:399931-399950 MsG0180000019.01.T01:CDS 40.0%
AGCACAGGATTCAACTGAAG+AGG + Chr1:400287-400306 None:intergenic 45.0%
GGATTGCTTTACTGATGGAG+AGG - Chr1:400121-400140 MsG0180000019.01.T01:CDS 45.0%
GGTGCAAAAACTCATGCTAC+TGG - Chr1:399684-399703 MsG0180000019.01.T01:CDS 45.0%
TCATTGCCACCACCATCTTT+AGG + Chr1:399765-399784 None:intergenic 45.0%
TGATCTCTTCCTGGACTGAA+AGG + Chr1:399630-399649 None:intergenic 45.0%
TTGCTTTACTGATGGAGAGG+AGG - Chr1:400124-400143 MsG0180000019.01.T01:CDS 45.0%
TTTACCTCCGTATCCACCTT+TGG + Chr1:399900-399919 None:intergenic 45.0%
! GCTACTGGTAAGGGATTTTC+TGG - Chr1:399699-399718 MsG0180000019.01.T01:CDS 45.0%
!!! GCACCTATTTTAGGTGTTGG+TGG - Chr1:400170-400189 MsG0180000019.01.T01:CDS 45.0%
CAAAACGCTCCTTTCAGTCC+AGG - Chr1:399618-399637 MsG0180000019.01.T01:CDS 50.0%
CACTCCACCAAAGGTGGATA+CGG - Chr1:399890-399909 MsG0180000019.01.T01:CDS 50.0%
GAAGAACAATGGCTCCACCT+AGG - Chr1:400261-400280 MsG0180000019.01.T01:CDS 50.0%
GAGATTTGCAAGTGGACGTG+AGG - Chr1:400043-400062 MsG0180000019.01.T01:CDS 50.0%
GGATTCAACTGAAGAGGCCT+AGG + Chr1:400281-400300 None:intergenic 50.0%
TTCAACTGAAGAGGCCTAGG+TGG + Chr1:400278-400297 None:intergenic 50.0%
!! AACAACAAAGCACCGCCGAT+GGG - Chr1:400011-400030 MsG0180000019.01.T01:CDS 50.0%
ACCTCCGTATCCACCTTTGG+TGG + Chr1:399897-399916 None:intergenic 55.0%
CACCTTTGGTGGAGTGGACA+CGG + Chr1:399886-399905 None:intergenic 55.0%
CGTATCCACCTTTGGTGGAG+TGG + Chr1:399892-399911 None:intergenic 55.0%
CTCCTCATATCACCCATCGG+CGG + Chr1:400026-400045 None:intergenic 55.0%
TCCACCAAAGGTGGATACGG+AGG - Chr1:399893-399912 MsG0180000019.01.T01:CDS 55.0%
TTCCGTGTCCACTCCACCAA+AGG - Chr1:399881-399900 MsG0180000019.01.T01:CDS 55.0%
!! GAACAACAAAGCACCGCCGA+TGG - Chr1:400010-400029 MsG0180000019.01.T01:CDS 55.0%
CACCGCCGATGGGTGATATG+AGG - Chr1:400021-400040 MsG0180000019.01.T01:CDS 60.0%
CGTGTCCACTCCACCAAAGG+TGG - Chr1:399884-399903 MsG0180000019.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 399555 400319 399555 ID=MsG0180000019.01;Name=MsG0180000019.01
Chr1 mRNA 399555 400319 399555 ID=MsG0180000019.01.T01;Parent=MsG0180000019.01;Name=MsG0180000019.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|254
Chr1 exon 399555 400319 399555 ID=MsG0180000019.01.T01:exon:7904;Parent=MsG0180000019.01.T01
Chr1 CDS 399555 400319 399555 ID=MsG0180000019.01.T01:cds;Parent=MsG0180000019.01.T01
Gene Sequence

>MsG0180000019.01.T01

ATGGAGAAAGAGAAACAAAGTAAGCTAAGGAACAAGTTCATGAAAGCAATCACAATTACATTTCAAAACGCTCCTTTCAGTCCAGGAAGAGATCACAAATTTAGATCAGAAAATAACACAAAGTCATGGGGTGCAAAAACTCATGCTACTGGTAAGGGATTTTCTGGACCTATGGTTACTATGATTCCATATGAAGCTAGAAGAAAACCTAAAGATGGTGGTGGCAATGAAACTCAAGAACCTACTTCACCAAAGATTTCTTGTATGGGACAGATCAAGCATCACAAGAAGAAACATATTCCAAAACCCAACAAAGATCTCTTCACTTCCGTGTCCACTCCACCAAAGGTGGATACGGAGGTAAAGAGATCGAAGTTTCAGAGGATTTTTTCGCAGAGGTCTAAAGCGAAATTTGCGGAAAGGAAATCAGATGGTTCTGGATTTGTTGATGAAGGGAACAACAAAGCACCGCCGATGGGTGATATGAGGAGATTTGCAAGTGGACGTGAGGCTTTTTCTAAATTTGATTGGAAGGCAGTGATTGAGCCTGAAGAAATTGATCAAAGGGATTGCTTTACTGATGGAGAGGAGGATGAAATTTTGATTCCATTTTCTGCACCTATTTTAGGTGTTGGTGGAAATAGTGGTACATCTAATGTGGCTGATTTGAAGTTTAAGCCAAGGAATGAGATTAATCTTTGGAAGAGAAGAACAATGGCTCCACCTAGGCCTCTTCAGTTGAATCCTGTGCTTAAAGCTAAATAA

Protein sequence

>MsG0180000019.01.T01

MEKEKQSKLRNKFMKAITITFQNAPFSPGRDHKFRSENNTKSWGAKTHATGKGFSGPMVTMIPYEARRKPKDGGGNETQEPTSPKISCMGQIKHHKKKHIPKPNKDLFTSVSTPPKVDTEVKRSKFQRIFSQRSKAKFAERKSDGSGFVDEGNNKAPPMGDMRRFASGREAFSKFDWKAVIEPEEIDQRDCFTDGEEDEILIPFSAPILGVGGNSGTSNVADLKFKPRNEINLWKRRTMAPPRPLQLNPVLKAK*