AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043755.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043755.01.T01 MTR_8g040820 73.759 282 71 3 1 281 9 288 3.29e-148 419
MsG0880043755.01.T01 MTR_8g040490 71.277 282 77 3 1 281 9 287 2.23e-143 408
MsG0880043755.01.T01 MTR_8g035720 73.704 270 67 3 14 281 83 350 2.60e-138 396
MsG0880043755.01.T01 MTR_8g040430 69.258 283 83 3 1 281 9 289 1.64e-137 392
MsG0880043755.01.T01 MTR_7g007710 66.904 281 82 4 5 283 7 278 3.68e-126 363
MsG0880043755.01.T01 MTR_7g007680 64.158 279 93 3 4 281 5 277 3.10e-121 352
MsG0880043755.01.T01 MTR_8g040520 60.140 286 100 9 1 281 9 285 1.82e-107 316
MsG0880043755.01.T01 MTR_3g013500 56.140 285 110 6 1 283 12 283 2.35e-105 310
MsG0880043755.01.T01 MTR_8g041790 58.214 280 91 6 5 281 13 269 1.50e-100 297
MsG0880043755.01.T01 MTR_8g014380 55.357 280 79 7 4 281 12 247 4.89e-93 276
MsG0880043755.01.T01 MTR_8g040250 56.716 268 82 3 15 281 5 239 3.91e-92 275
MsG0880043755.01.T01 MTR_8g040820 75.301 166 40 1 117 281 24 189 7.61e-85 254
MsG0880043755.01.T01 MTR_0071s0010 46.332 259 133 4 26 282 24 278 3.60e-83 253
MsG0880043755.01.T01 MTR_3g046670 46.332 259 133 4 26 282 24 278 3.60e-83 253
MsG0880043755.01.T01 MTR_8g066220 47.656 256 129 3 28 282 27 278 4.29e-83 253
MsG0880043755.01.T01 MTR_3g435130 44.574 258 137 4 27 282 26 279 2.05e-79 244
MsG0880043755.01.T01 MTR_8g040430 72.393 163 44 1 120 281 27 189 2.10e-78 238
MsG0880043755.01.T01 MTR_8g066210 44.747 257 122 3 27 282 26 263 5.49e-77 237
MsG0880043755.01.T01 MTR_8g040730 48.347 242 75 9 1 238 1 196 1.44e-54 185
MsG0880043755.01.T01 MTR_8g040730 59.783 184 65 7 1 183 324 499 7.05e-54 183
MsG0880043755.01.T01 MTR_8g066280 47.368 152 75 3 135 282 4 154 2.99e-45 152
MsG0880043755.01.T01 MTR_3g434790 46.094 128 67 2 156 282 53 179 1.29e-35 128
MsG0880043755.01.T01 MTR_8g040710 56.311 103 43 2 181 281 134 236 1.69e-32 121
MsG0880043755.01.T01 MTR_8g040315 87.324 71 8 1 213 282 8 78 2.23e-28 106
MsG0880043755.01.T01 MTR_8g040410 67.797 59 17 1 68 124 22 80 1.13e-19 83.6
MsG0880043755.01.T01 MTR_8g040410 82.609 46 7 1 229 273 82 127 4.98e-18 79.3
MsG0880043755.01.T01 MTR_8g066190 52.830 53 24 1 230 281 2 54 2.01e-11 59.7
MsG0880043755.01.T01 MTR_8g038710 57.407 54 22 1 135 187 11 64 2.93e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043755.01.T01 AT5G07010 47.232 271 134 5 15 282 37 301 8.41e-90 271
MsG0880043755.01.T01 AT5G07000 47.529 263 128 5 25 282 32 289 1.02e-85 260
MsG0880043755.01.T01 AT3G45070 42.701 274 144 4 11 283 8 269 7.43e-74 229
MsG0880043755.01.T01 AT3G45070 42.701 274 144 4 11 283 13 274 7.49e-74 229
MsG0880043755.01.T01 AT3G45070 42.701 274 144 4 11 283 40 301 1.65e-73 229
MsG0880043755.01.T01 AT2G03770 42.636 258 135 4 26 282 22 267 1.38e-68 216
MsG0880043755.01.T01 AT1G18590 44.656 262 133 6 24 282 32 284 7.47e-68 214
MsG0880043755.01.T01 AT1G13420 43.191 257 138 4 28 283 28 277 6.37e-67 212
MsG0880043755.01.T01 AT3G45080 41.245 257 146 3 28 283 22 274 4.77e-65 207
MsG0880043755.01.T01 AT2G14920 41.473 258 148 3 28 283 24 280 3.49e-64 204
MsG0880043755.01.T01 AT5G43690 39.847 261 146 7 28 283 23 277 5.62e-63 201
MsG0880043755.01.T01 AT1G74100 41.603 262 139 8 25 282 25 276 3.71e-61 197
MsG0880043755.01.T01 AT1G28170 41.573 267 150 4 18 282 9 271 8.00e-61 196
MsG0880043755.01.T01 AT1G13430 40.684 263 135 4 28 272 28 287 3.57e-59 192
MsG0880043755.01.T01 AT2G03760 44.358 257 131 6 28 282 23 269 1.05e-58 190
MsG0880043755.01.T01 AT4G26280 37.452 259 137 4 28 283 19 255 9.46e-57 185
MsG0880043755.01.T01 AT2G03750 37.313 268 160 5 16 282 35 295 5.79e-55 181
MsG0880043755.01.T01 AT1G74090 42.529 261 138 6 25 282 37 288 4.09e-54 179
MsG0880043755.01.T01 AT2G27570 33.730 252 120 4 28 276 22 229 6.46e-44 150

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGGTCAATTTGAAAATT+TGG 0.159871 8:-30125321 None:intergenic
TAAAAGTTTAATTCAAAATA+AGG 0.190833 8:-30125282 None:intergenic
TTGATACATTTGTGTCATAT+TGG 0.271726 8:+30125451 MsG0880043755.01.T01:CDS
AACCATGTAGTGCCCCATTT+TGG 0.277009 8:-30125165 None:intergenic
TTGTAGCCTCCATGCCAAAA+TGG 0.281020 8:+30125151 MsG0880043755.01.T01:CDS
GCAAAATAATTTATATATAT+AGG 0.292858 8:+30125421 MsG0880043755.01.T01:CDS
TTCAAGAATAAAATCTTATT+AGG 0.305417 8:-30125618 None:intergenic
TTTCTTGTTCACCCCTTGAT+TGG 0.315825 8:-30124942 None:intergenic
ATGACACAAATGTATCAAAT+TGG 0.339400 8:-30125446 None:intergenic
TTGTCACTTCCTAAAGAAAA+TGG 0.342211 8:+30125018 MsG0880043755.01.T01:CDS
ACTCAAGAAGAGGAAAATAA+TGG 0.377886 8:+30125708 MsG0880043755.01.T01:CDS
AAAAGTTTAATTCAAAATAA+GGG 0.394335 8:-30125281 None:intergenic
CAAAAGAATTGCAGAGTTTG+TGG 0.398800 8:+30125674 MsG0880043755.01.T01:CDS
TTTGAAAAGAGTTTATTGAT+TGG 0.400016 8:-30125098 None:intergenic
TGTTAAAATTAACATCTTTG+AGG 0.400392 8:-30125650 None:intergenic
ATTGATTGGATGAGAGTTGA+TGG 0.406498 8:-30125084 None:intergenic
TTGGTTCAATCATGTTTGAT+AGG 0.413833 8:-30125338 None:intergenic
TGTAGCCTCCATGCCAAAAT+GGG 0.428888 8:+30125152 MsG0880043755.01.T01:CDS
TAAGTGCGTATGGACCGTTT+TGG 0.444155 8:+30125556 MsG0880043755.01.T01:CDS
GGTTAACAATGGCATATGTA+AGG 0.461890 8:-30125194 None:intergenic
AAAAGAATTGCAGAGTTTGT+GGG 0.464930 8:+30125675 MsG0880043755.01.T01:CDS
ACACATTCCATTCTCTTCGT+TGG 0.466230 8:+30125374 MsG0880043755.01.T01:CDS
TCATATGTTGGGTTGCTTGA+AGG 0.474585 8:+30125581 MsG0880043755.01.T01:CDS
CAAAGGAGGGAAAATGTTTG+CGG 0.484909 8:-30125215 None:intergenic
ATTTCATCTATGGTTAACAT+AGG 0.493382 8:-30125510 None:intergenic
TGCAGCTTTGAGAGTATGAA+TGG 0.495135 8:+30125756 MsG0880043755.01.T01:CDS
AATTAATGAAGAAGATAAGC+TGG 0.503949 8:+30124966 MsG0880043755.01.T01:CDS
ATATGAATGGAAACCAATCA+AGG 0.508078 8:+30124929 None:intergenic
CACACATATGATGATATGAA+TGG 0.514461 8:+30124916 None:intergenic
GACTTAGCCAACGAAGAGAA+TGG 0.521939 8:-30125381 None:intergenic
TGCCAAAATGGGGCACTACA+TGG 0.533557 8:+30125163 MsG0880043755.01.T01:CDS
ATTTCCTTTCACTCAAGAAG+AGG 0.550723 8:+30125698 MsG0880043755.01.T01:CDS
TGTAATGGGATAAGTGCGTA+TGG 0.562143 8:+30125546 MsG0880043755.01.T01:CDS
AAAATGTTTGCGGTTAACAA+TGG 0.564835 8:-30125205 None:intergenic
AGAAGAGGAAAATAATGGAA+TGG 0.576707 8:+30125713 MsG0880043755.01.T01:CDS
GGATGGTCATTCTCAAAGGA+GGG 0.580828 8:-30125228 None:intergenic
GTTAACAATGGCATATGTAA+GGG 0.583627 8:-30125193 None:intergenic
CCCAACATATGATCCCAAAA+CGG 0.591413 8:-30125570 None:intergenic
TAATGGATGGTCATTCTCAA+AGG 0.594736 8:-30125232 None:intergenic
TATGAATGGAAACCAATCAA+GGG 0.595765 8:+30124930 None:intergenic
TGGATGGTCATTCTCAAAGG+AGG 0.596431 8:-30125229 None:intergenic
AAATAAGGGACTAATTCGTG+TGG 0.604696 8:-30125267 None:intergenic
TGTGGATTGAATGCAAGTAA+TGG 0.622386 8:-30125249 None:intergenic
ATGAATGGAAACCAATCAAG+GGG 0.638662 8:+30124931 MsG0880043755.01.T01:CDS
GTAGCCTCCATGCCAAAATG+GGG 0.673239 8:+30125153 MsG0880043755.01.T01:CDS
TATCAAACATGATTGAACCA+AGG 0.678125 8:+30125340 MsG0880043755.01.T01:CDS
GATTGAATGCAAGTAATGGA+TGG 0.685646 8:-30125245 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTTTAATTCAAAATAA+GGG - Chr8:30125284-30125303 None:intergenic 10.0%
!! ATGATAAAAATACAAATATT+GGG - Chr8:30125052-30125071 None:intergenic 10.0%
!! GCAAAATAATTTATATATAT+AGG + Chr8:30125421-30125440 MsG0880043755.01.T01:CDS 10.0%
!! TAAAAGTTTAATTCAAAATA+AGG - Chr8:30125285-30125304 None:intergenic 10.0%
!!! TATATAAATTATTTTGCAAT+TGG - Chr8:30125418-30125437 None:intergenic 10.0%
!!! TATTTTGAATTAAACTTTTA+TGG + Chr8:30125285-30125304 MsG0880043755.01.T01:CDS 10.0%
!! CATGATAAAAATACAAATAT+TGG - Chr8:30125053-30125072 None:intergenic 15.0%
!! TTCAAGAATAAAATCTTATT+AGG - Chr8:30125621-30125640 None:intergenic 15.0%
!!! ATTTTTGAAAAATACTGTAA+TGG + Chr8:30125531-30125550 MsG0880043755.01.T01:CDS 15.0%
!!! CAATATTTGTATTTTTATCA+TGG + Chr8:30125051-30125070 MsG0880043755.01.T01:CDS 15.0%
!!! TTTTTCAAAAATTTCATCTA+TGG - Chr8:30125523-30125542 None:intergenic 15.0%
!!! TTTTTGAAAAATACTGTAAT+GGG + Chr8:30125532-30125551 MsG0880043755.01.T01:CDS 15.0%
!! AAAAATACAAATATTGGGTT+AGG - Chr8:30125047-30125066 None:intergenic 20.0%
!! TGTTAAAATTAACATCTTTG+AGG - Chr8:30125653-30125672 None:intergenic 20.0%
!! TTTGAAAAGAGTTTATTGAT+TGG - Chr8:30125101-30125120 None:intergenic 20.0%
!!! TGTATTTTTATCATGGATTT+TGG + Chr8:30125058-30125077 MsG0880043755.01.T01:CDS 20.0%
! AATTAATGAAGAAGATAAGC+TGG + Chr8:30124966-30124985 MsG0880043755.01.T01:CDS 25.0%
! ATGACACAAATGTATCAAAT+TGG - Chr8:30125449-30125468 None:intergenic 25.0%
! ATTTCATCTATGGTTAACAT+AGG - Chr8:30125513-30125532 None:intergenic 25.0%
! GATAGGTCAATTTGAAAATT+TGG - Chr8:30125324-30125343 None:intergenic 25.0%
! TTGATACATTTGTGTCATAT+TGG + Chr8:30125451-30125470 MsG0880043755.01.T01:CDS 25.0%
AAAAGAATTGCAGAGTTTGT+GGG + Chr8:30125675-30125694 MsG0880043755.01.T01:CDS 30.0%
AAAATGTTTGCGGTTAACAA+TGG - Chr8:30125208-30125227 None:intergenic 30.0%
ACTCAAGAAGAGGAAAATAA+TGG + Chr8:30125708-30125727 MsG0880043755.01.T01:CDS 30.0%
GTTAACAATGGCATATGTAA+GGG - Chr8:30125196-30125215 None:intergenic 30.0%
TATCAAACATGATTGAACCA+AGG + Chr8:30125340-30125359 MsG0880043755.01.T01:CDS 30.0%
TTGGTTCAATCATGTTTGAT+AGG - Chr8:30125341-30125360 None:intergenic 30.0%
! AACTTTTATGGTGATAGAGA+TGG + Chr8:30125297-30125316 MsG0880043755.01.T01:CDS 30.0%
! AGAAGAGGAAAATAATGGAA+TGG + Chr8:30125713-30125732 MsG0880043755.01.T01:CDS 30.0%
! TTGTCACTTCCTAAAGAAAA+TGG + Chr8:30125018-30125037 MsG0880043755.01.T01:CDS 30.0%
AAATAAGGGACTAATTCGTG+TGG - Chr8:30125270-30125289 None:intergenic 35.0%
ATGAATGGAAACCAATCAAG+GGG + Chr8:30124931-30124950 MsG0880043755.01.T01:CDS 35.0%
ATGTGTGTTACAAAAAGCCT+TGG - Chr8:30125360-30125379 None:intergenic 35.0%
ATTGATTGGATGAGAGTTGA+TGG - Chr8:30125087-30125106 None:intergenic 35.0%
ATTTCCTTTCACTCAAGAAG+AGG + Chr8:30125698-30125717 MsG0880043755.01.T01:CDS 35.0%
CAAAAGAATTGCAGAGTTTG+TGG + Chr8:30125674-30125693 MsG0880043755.01.T01:CDS 35.0%
GGTTAACAATGGCATATGTA+AGG - Chr8:30125197-30125216 None:intergenic 35.0%
! GATTGAATGCAAGTAATGGA+TGG - Chr8:30125248-30125267 None:intergenic 35.0%
! GTTAGGTGACCATTTTCTTT+AGG - Chr8:30125030-30125049 None:intergenic 35.0%
! TAATGGATGGTCATTCTCAA+AGG - Chr8:30125235-30125254 None:intergenic 35.0%
! TGTGGATTGAATGCAAGTAA+TGG - Chr8:30125252-30125271 None:intergenic 35.0%
! TTTTCCTCTTCTTGAGTGAA+AGG - Chr8:30125705-30125724 None:intergenic 35.0%
ACACATTCCATTCTCTTCGT+TGG + Chr8:30125374-30125393 MsG0880043755.01.T01:CDS 40.0%
CAAAGGAGGGAAAATGTTTG+CGG - Chr8:30125218-30125237 None:intergenic 40.0%
CCCAACATATGATCCCAAAA+CGG - Chr8:30125573-30125592 None:intergenic 40.0%
TTTCTTGTTCACCCCTTGAT+TGG - Chr8:30124945-30124964 None:intergenic 40.0%
! TCATATGTTGGGTTGCTTGA+AGG + Chr8:30125581-30125600 MsG0880043755.01.T01:CDS 40.0%
! TGTAATGGGATAAGTGCGTA+TGG + Chr8:30125546-30125565 MsG0880043755.01.T01:CDS 40.0%
!! TGCAGCTTTGAGAGTATGAA+TGG + Chr8:30125756-30125775 MsG0880043755.01.T01:CDS 40.0%
!!! ACCGTTTTGGGATCATATGT+TGG + Chr8:30125569-30125588 MsG0880043755.01.T01:CDS 40.0%
!!! CCGTTTTGGGATCATATGTT+GGG + Chr8:30125570-30125589 MsG0880043755.01.T01:CDS 40.0%
GACTTAGCCAACGAAGAGAA+TGG - Chr8:30125384-30125403 None:intergenic 45.0%
GGATGGTCATTCTCAAAGGA+GGG - Chr8:30125231-30125250 None:intergenic 45.0%
TGGATGGTCATTCTCAAAGG+AGG - Chr8:30125232-30125251 None:intergenic 45.0%
TGTAGCCTCCATGCCAAAAT+GGG + Chr8:30125152-30125171 MsG0880043755.01.T01:CDS 45.0%
TTGTAGCCTCCATGCCAAAA+TGG + Chr8:30125151-30125170 MsG0880043755.01.T01:CDS 45.0%
! AACCATGTAGTGCCCCATTT+TGG - Chr8:30125168-30125187 None:intergenic 45.0%
! AAGTGCGTATGGACCGTTTT+GGG + Chr8:30125557-30125576 MsG0880043755.01.T01:CDS 45.0%
! TAAGTGCGTATGGACCGTTT+TGG + Chr8:30125556-30125575 MsG0880043755.01.T01:CDS 45.0%
GTAGCCTCCATGCCAAAATG+GGG + Chr8:30125153-30125172 MsG0880043755.01.T01:CDS 50.0%
! TGCCAAAATGGGGCACTACA+TGG + Chr8:30125163-30125182 MsG0880043755.01.T01:CDS 50.0%
!! TGTAGTGCCCCATTTTGGCA+TGG - Chr8:30125163-30125182 None:intergenic 50.0%
!! AGTGCCCCATTTTGGCATGG+AGG - Chr8:30125160-30125179 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 30124931 30125782 30124931 ID=MsG0880043755.01;Name=MsG0880043755.01
Chr8 mRNA 30124931 30125782 30124931 ID=MsG0880043755.01.T01;Parent=MsG0880043755.01;Name=MsG0880043755.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|283
Chr8 exon 30124931 30125782 30124931 ID=MsG0880043755.01.T01:exon:7153;Parent=MsG0880043755.01.T01
Chr8 CDS 30124931 30125782 30124931 ID=MsG0880043755.01.T01:cds;Parent=MsG0880043755.01.T01
Gene Sequence

>MsG0880043755.01.T01

ATGAATGGAAACCAATCAAGGGGTGAACAAGAAAAAATTAATGAAGAAGATAAGCTGGTAAGTAATGATTATTATAATCAGTTAATCTTGTCACTTCCTAAAGAAAATGGTCACCTAACCCAATATTTGTATTTTTATCATGGATTTTGGTGTCCATCAACTCTCATCCAATCAATAAACTCTTTTCAAAATTGTTTTAATGCAAAAGAGAGTGATATTGTTGTAGCCTCCATGCCAAAATGGGGCACTACATGGTTGTCAGCCCTTACATATGCCATTGTTAACCGCAAACATTTTCCCTCCTTTGAGAATGACCATCCATTACTTGCATTCAATCCACACGAATTAGTCCCTTATTTTGAATTAAACTTTTATGGTGATAGAGATGGTCCAAATTTTCAAATTGACCTATCAAACATGATTGAACCAAGGCTTTTTGTAACACACATTCCATTCTCTTCGTTGGCTAAGTCAGTTAAAGAGTCCAATTGCAAAATAATTTATATATATAGGAACCAATTTGATACATTTGTGTCATATTGGAATTTCAACAACAAAATTAGATTAAAGATGTCTTTACCTATGTTAACCATAGATGAAATTTTTGAAAAATACTGTAATGGGATAAGTGCGTATGGACCGTTTTGGGATCATATGTTGGGTTGCTTGAAGGAGAGCATAGTCAGACCTAATAAGATTTTATTCTTGAAATATGAAGACCTCAAAGATGTTAATTTTAACATCAAAAGAATTGCAGAGTTTGTGGGATTTCCTTTCACTCAAGAAGAGGAAAATAATGGAATGGTTGAAAATATAATCAAGTTATGCAGCTTTGAGAGTATGAATGGTTGA

Protein sequence

>MsG0880043755.01.T01

MNGNQSRGEQEKINEEDKLVSNDYYNQLILSLPKENGHLTQYLYFYHGFWCPSTLIQSINSFQNCFNAKESDIVVASMPKWGTTWLSALTYAIVNRKHFPSFENDHPLLAFNPHELVPYFELNFYGDRDGPNFQIDLSNMIEPRLFVTHIPFSSLAKSVKESNCKIIYIYRNQFDTFVSYWNFNNKIRLKMSLPMLTIDEIFEKYCNGISAYGPFWDHMLGCLKESIVRPNKILFLKYEDLKDVNFNIKRIAEFVGFPFTQEEENNGMVENIIKLCSFESMNG*