AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044704.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044704.01.T01 MTR_8g467340 91.667 84 5 2 1 83 297 379 2.88e-46 152
MsG0880044704.01.T01 MTR_7g010300 38.372 86 47 2 1 83 246 328 1.47e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCATGTTTCTTCTGTTAA+TGG 0.120548 8:+47844193 MsG0880044704.01.T01:CDS
TCAGAATCTGATGTTGGAAT+TGG 0.361684 8:+47844056 MsG0880044704.01.T01:CDS
GCAAAAGAGTGATGATGGTT+TGG 0.463952 8:+47843944 MsG0880044704.01.T01:CDS
CAGAAATTCTGCAATGACAT+TGG 0.496065 8:+47843969 MsG0880044704.01.T01:CDS
CATATGATGAATGACATTGT+TGG 0.516395 8:-47844150 None:intergenic
ATTCATCATATGAATGAAGA+TGG 0.518033 8:+47844161 MsG0880044704.01.T01:CDS
AAGAAATCAGAATCTGATGT+TGG 0.519643 8:+47844050 MsG0880044704.01.T01:CDS
AAAATGCAAAAGAGTGATGA+TGG 0.531947 8:+47843939 None:intergenic
TTCATCATATGAATGAAGAT+GGG 0.583881 8:+47844162 MsG0880044704.01.T01:CDS
GCTGCTAATGGTAATGATGA+TGG 0.596655 8:+47844125 MsG0880044704.01.T01:CDS
AATGACATTGGTGTTTCTAG+AGG 0.607705 8:+47843981 MsG0880044704.01.T01:CDS
ACAACAAGAATAGTAGCTTG+AGG 0.648719 8:+47844027 MsG0880044704.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTCATCATATGAATGAAGA+TGG + Chr8:47844161-47844180 MsG0880044704.01.T01:CDS 25.0%
! GATAAAAATAATGCTGCTAA+TGG + Chr8:47844113-47844132 MsG0880044704.01.T01:CDS 25.0%
! TTCATCATATGAATGAAGAT+GGG + Chr8:47844162-47844181 MsG0880044704.01.T01:CDS 25.0%
AAGAAATCAGAATCTGATGT+TGG + Chr8:47844050-47844069 MsG0880044704.01.T01:CDS 30.0%
CATATGATGAATGACATTGT+TGG - Chr8:47844153-47844172 None:intergenic 30.0%
GTTCATGTTTCTTCTGTTAA+TGG + Chr8:47844193-47844212 MsG0880044704.01.T01:CDS 30.0%
ACAACAAGAATAGTAGCTTG+AGG + Chr8:47844027-47844046 MsG0880044704.01.T01:CDS 35.0%
CAGAAATTCTGCAATGACAT+TGG + Chr8:47843969-47843988 MsG0880044704.01.T01:CDS 35.0%
TCAGAATCTGATGTTGGAAT+TGG + Chr8:47844056-47844075 MsG0880044704.01.T01:CDS 35.0%
! CTAGAGGAGTTTTCAAAGTT+TGG + Chr8:47843997-47844016 MsG0880044704.01.T01:CDS 35.0%
!! AATGACATTGGTGTTTCTAG+AGG + Chr8:47843981-47844000 MsG0880044704.01.T01:CDS 35.0%
! GCTGCTAATGGTAATGATGA+TGG + Chr8:47844125-47844144 MsG0880044704.01.T01:CDS 40.0%
!! GCAAAAGAGTGATGATGGTT+TGG + Chr8:47843944-47843963 MsG0880044704.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
Chr8 gene 47843942 47844235 47843942 ID=MsG0880044704.01;Name=MsG0880044704.01
Chr8 mRNA 47843942 47844235 47843942 ID=MsG0880044704.01.T01;Parent=MsG0880044704.01;Name=MsG0880044704.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|97
Chr8 exon 47843942 47844235 47843942 ID=MsG0880044704.01.T01:exon:19581;Parent=MsG0880044704.01.T01
Chr8 CDS 47843942 47844235 47843942 ID=MsG0880044704.01.T01:cds;Parent=MsG0880044704.01.T01
Gene Sequence

>MsG0880044704.01.T01

ATGCAAAAGAGTGATGATGGTTTGGTTCAGAAATTCTGCAATGACATTGGTGTTTCTAGAGGAGTTTTCAAAGTTTGGATGCATAACAACAAGAATAGTAGCTTGAGGAAGAAATCAGAATCTGATGTTGGAATTGGAATTGCTGCTACTCAAATTGCTACTCAAAATGATGATAAAAATAATGCTGCTAATGGTAATGATGATGGTTCCAACAATGTCATTCATCATATGAATGAAGATGGGTGTGTCATGTTCATGTTTCTTCTGTTAATGGTTTATCCTCTTCTTAATTAG

Protein sequence

>MsG0880044704.01.T01

MQKSDDGLVQKFCNDIGVSRGVFKVWMHNNKNSSLRKKSESDVGIGIAATQIATQNDDKNNAANGNDDGSNNVIHHMNEDGCVMFMFLLLMVYPLLN*