AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019340.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019340.01.T01 MTR_4g029390 89.474 114 11 1 1 114 1 113 3.53e-68 201
MsG0480019340.01.T01 MTR_4g029350 81.579 114 20 1 1 114 1 113 3.22e-62 186
MsG0480019340.01.T01 MTR_4g428370 71.681 113 30 1 1 113 58 168 3.45e-50 157
MsG0480019340.01.T01 MTR_4g027840 68.142 113 34 1 1 113 1 111 1.74e-47 149
MsG0480019340.01.T01 MTR_4g027800 68.142 113 34 1 1 113 1 111 1.86e-47 148
MsG0480019340.01.T01 MTR_4g029210 50.000 118 57 2 1 118 1 116 1.38e-29 103
MsG0480019340.01.T01 MTR_7g072930 43.220 118 64 2 2 117 3 119 4.23e-25 93.2
MsG0480019340.01.T01 MTR_2g062600 42.241 116 63 3 1 115 1 113 5.37e-25 91.7
MsG0480019340.01.T01 MTR_4g028180 49.550 111 48 4 11 114 14 123 1.17e-24 91.3
MsG0480019340.01.T01 MTR_4g028190 38.983 118 70 2 1 117 5 121 4.02e-23 87.4
MsG0480019340.01.T01 MTR_7g073150 42.609 115 61 3 4 116 7 118 3.07e-22 85.1
MsG0480019340.01.T01 MTR_7g072830 33.898 118 72 2 4 115 1 118 2.43e-21 82.8
MsG0480019340.01.T01 MTR_7g073170 40.566 106 60 3 11 115 13 116 2.94e-21 82.4
MsG0480019340.01.T01 MTR_7g072730 34.677 124 78 2 2 122 3 126 3.32e-21 83.6
MsG0480019340.01.T01 MTR_3g467100 35.135 111 71 1 5 115 3 112 1.76e-20 80.1
MsG0480019340.01.T01 MTR_4g028250 37.069 116 72 1 1 115 1 116 9.94e-20 78.6
MsG0480019340.01.T01 MTR_7g072993 35.484 124 77 1 2 122 3 126 5.04e-19 77.0
MsG0480019340.01.T01 MTR_7g073120 33.065 124 80 2 2 122 3 126 5.51e-19 77.4
MsG0480019340.01.T01 MTR_7g072980 30.894 123 83 1 2 122 3 125 9.62e-18 73.9
MsG0480019340.01.T01 MTR_7g073130 34.426 122 77 3 2 122 3 122 2.08e-17 72.8
MsG0480019340.01.T01 MTR_7g072900 34.783 115 74 1 2 115 3 117 2.30e-15 67.4
MsG0480019340.01.T01 MTR_7g072960 37.607 117 56 3 3 117 4 105 4.33e-15 67.0
MsG0480019340.01.T01 MTR_7g072810 35.965 114 64 3 11 122 13 119 5.54e-15 66.6
MsG0480019340.01.T01 MTR_4g028310 31.897 116 74 3 1 113 1 114 1.16e-14 65.9
MsG0480019340.01.T01 MTR_3g056640 33.613 119 73 4 1 114 2 119 1.82e-14 65.1
MsG0480019340.01.T01 MTR_1g040335 36.207 116 71 2 2 115 3 117 3.17e-14 64.3
MsG0480019340.01.T01 MTR_7g451500 33.333 102 62 2 15 115 1 97 5.47e-14 63.2
MsG0480019340.01.T01 MTR_7g073100 33.333 108 71 1 16 122 17 124 5.55e-14 64.3
MsG0480019340.01.T01 MTR_3g046515 29.825 114 76 3 4 114 7 119 9.29e-14 63.2
MsG0480019340.01.T01 MTR_3g046350 32.000 100 63 4 8 103 10 108 1.15e-13 62.8
MsG0480019340.01.T01 MTR_3g046530 29.825 114 76 3 4 114 7 119 2.61e-13 62.0
MsG0480019340.01.T01 MTR_3g464130 35.556 90 55 2 27 114 18 106 4.09e-13 61.2
MsG0480019340.01.T01 MTR_3g046540 29.825 114 76 3 4 114 7 119 5.71e-13 61.2
MsG0480019340.01.T01 MTR_7g073060 32.174 115 77 1 2 115 3 117 2.97e-12 59.3
MsG0480019340.01.T01 MTR_3g464110 38.272 81 47 2 36 114 93 172 4.54e-12 60.1
MsG0480019340.01.T01 MTR_7g072720 31.200 125 78 4 1 119 1 123 2.38e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019340.01.T01 AT5G59320 46.729 107 55 1 8 114 8 112 3.83e-27 97.4
MsG0480019340.01.T01 AT5G59310 46.226 106 52 2 8 113 8 108 1.15e-25 93.6
MsG0480019340.01.T01 AT3G51590 42.478 113 61 2 6 114 1 113 1.64e-23 88.2
MsG0480019340.01.T01 AT2G38540 50.000 104 49 3 12 113 12 114 3.69e-23 87.4
MsG0480019340.01.T01 AT3G51600 48.571 105 51 3 12 114 12 115 1.73e-22 85.5
MsG0480019340.01.T01 AT2G38530 48.077 104 53 1 12 114 12 115 2.29e-22 85.1
MsG0480019340.01.T01 AT4G33355 46.667 90 48 0 26 115 28 117 2.43e-22 85.1
MsG0480019340.01.T01 AT5G01870 43.925 107 54 4 13 114 8 113 1.06e-21 83.6
MsG0480019340.01.T01 AT4G33355 46.067 89 48 0 26 114 28 116 2.11e-21 82.8
MsG0480019340.01.T01 AT3G08770 43.617 94 49 3 24 114 18 110 3.47e-19 77.0
MsG0480019340.01.T01 AT3G08770 43.617 94 49 3 24 114 18 110 4.33e-19 76.6
MsG0480019340.01.T01 AT2G15050 38.393 112 63 4 12 117 11 122 1.61e-18 75.5
MsG0480019340.01.T01 AT2G15050 38.532 109 61 4 12 114 11 119 2.78e-17 72.4
MsG0480019340.01.T01 AT2G15050 38.835 103 59 3 12 110 11 113 6.52e-17 71.2
MsG0480019340.01.T01 AT2G18370 37.634 93 56 2 23 114 22 113 8.28e-16 68.6

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGTTGCAAGCAGCCTTGC+GGG 0.246298 4:-18665412 None:intergenic
GCACTTCTACTAACTGTAAT+AGG 0.249879 4:+18665529 MsG0480019340.01.T01:CDS
TTGCAAGCAGCCTTGCGGGC+AGG 0.354252 4:-18665408 None:intergenic
CCATGCATTACTTATCTTCA+AGG 0.382464 4:+18665318 MsG0480019340.01.T01:CDS
CTTGCGGGCAGGGCCGTTGT+TGG 0.386162 4:-18665397 None:intergenic
AGTAGAAGTGCTAAACTTGT+AGG 0.409303 4:-18665517 None:intergenic
ACTTATCTTCAAGGTGGTGC+TGG 0.410647 4:+18665327 MsG0480019340.01.T01:CDS
GTGACTGCTGCCTCTGCCAT+TGG 0.435322 4:-18665267 None:intergenic
GATAAGTAATGCATGGAGTA+AGG 0.447792 4:-18665311 None:intergenic
CAACAACGGCCCTGCCCGCA+AGG 0.458714 4:+18665398 MsG0480019340.01.T01:CDS
GCGGGCAGGGCCGTTGTTGG+CGG 0.471199 4:-18665394 None:intergenic
TGCAAGCAGCCTTGCGGGCA+GGG 0.476120 4:-18665407 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.487551 4:+18665429 MsG0480019340.01.T01:CDS
ATGTTAACACCACATTTGCC+AGG 0.490963 4:-18665492 None:intergenic
GCTGCTCTCCCTGGCAAATG+TGG 0.495611 4:+18665483 MsG0480019340.01.T01:CDS
AGCAGTTGCAAGCAGCCTTG+CGG 0.500292 4:-18665413 None:intergenic
GTTGTTATTCAAACGAGCAA+TGG 0.508618 4:-18665454 None:intergenic
AACCAAGCTGCTGCTCTCCC+TGG 0.514408 4:+18665474 MsG0480019340.01.T01:CDS
GGTGGAGTGAGGAGACTTAA+CGG 0.519956 4:+18665369 MsG0480019340.01.T01:CDS
TGCCAGGGAGAGCAGCAGCT+TGG 0.525683 4:-18665476 None:intergenic
AAGCATGAGAATTGCATTTG+TGG 0.526489 4:+18665212 MsG0480019340.01.T01:CDS
GGTCCATCTGCAGCATGCTG+TGG 0.539612 4:+18665348 MsG0480019340.01.T01:CDS
CCATCTGCAGCATGCTGTGG+TGG 0.550747 4:+18665351 MsG0480019340.01.T01:CDS
GTAGAAGTGCTAAACTTGTA+GGG 0.551668 4:-18665516 None:intergenic
GTCACATGTGGACAAGTAGT+AGG 0.553840 4:+18665285 MsG0480019340.01.T01:CDS
GGTTAGTGCACCAATGGCAG+AGG 0.566893 4:+18665257 MsG0480019340.01.T01:CDS
CCACCACAGCATGCTGCAGA+TGG 0.580113 4:-18665351 None:intergenic
TGCAATTATGTGCATGATGG+TGG 0.600070 4:+18665236 MsG0480019340.01.T01:CDS
CTTAACGGTGCCGCCAACAA+CGG 0.605014 4:+18665384 MsG0480019340.01.T01:CDS
CCTTGAAGATAAGTAATGCA+TGG 0.607580 4:-18665318 None:intergenic
GATGGTGGTTAGTGCACCAA+TGG 0.623706 4:+18665251 MsG0480019340.01.T01:CDS
GGCTGCAATTATGTGCATGA+TGG 0.625253 4:+18665233 MsG0480019340.01.T01:CDS
GCAGAGGCAGCAGTCACATG+TGG 0.630368 4:+18665273 MsG0480019340.01.T01:CDS
TGTTAACACCACATTTGCCA+GGG 0.642773 4:-18665491 None:intergenic
CAGCATGCTGTGGTGGAGTG+AGG 0.648040 4:+18665358 MsG0480019340.01.T01:CDS
TGCATTACTTATCTTCAAGG+TGG 0.657751 4:+18665321 MsG0480019340.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCATGAGAATTGCATTTG+TGG + Chr4:18665212-18665231 MsG0480019340.01.T01:CDS 35.0%
CCATGCATTACTTATCTTCA+AGG + Chr4:18665318-18665337 MsG0480019340.01.T01:CDS 35.0%
CCTTGAAGATAAGTAATGCA+TGG - Chr4:18665321-18665340 None:intergenic 35.0%
GATAAGTAATGCATGGAGTA+AGG - Chr4:18665314-18665333 None:intergenic 35.0%
GCACTTCTACTAACTGTAAT+AGG + Chr4:18665529-18665548 MsG0480019340.01.T01:CDS 35.0%
TGCATTACTTATCTTCAAGG+TGG + Chr4:18665321-18665340 MsG0480019340.01.T01:CDS 35.0%
! GTTGTTATTCAAACGAGCAA+TGG - Chr4:18665457-18665476 None:intergenic 35.0%
!! AGTAGAAGTGCTAAACTTGT+AGG - Chr4:18665520-18665539 None:intergenic 35.0%
!! GTAGAAGTGCTAAACTTGTA+GGG - Chr4:18665519-18665538 None:intergenic 35.0%
ATGTTAACACCACATTTGCC+AGG - Chr4:18665495-18665514 None:intergenic 40.0%
TGCAATTATGTGCATGATGG+TGG + Chr4:18665236-18665255 MsG0480019340.01.T01:CDS 40.0%
TGTTAACACCACATTTGCCA+GGG - Chr4:18665494-18665513 None:intergenic 40.0%
ACTTATCTTCAAGGTGGTGC+TGG + Chr4:18665327-18665346 MsG0480019340.01.T01:CDS 45.0%
GGCTGCAATTATGTGCATGA+TGG + Chr4:18665233-18665252 MsG0480019340.01.T01:CDS 45.0%
GTCACATGTGGACAAGTAGT+AGG + Chr4:18665285-18665304 MsG0480019340.01.T01:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + Chr4:18665429-18665448 MsG0480019340.01.T01:CDS 45.0%
GGTGGAGTGAGGAGACTTAA+CGG + Chr4:18665369-18665388 MsG0480019340.01.T01:CDS 50.0%
! GATGGTGGTTAGTGCACCAA+TGG + Chr4:18665251-18665270 MsG0480019340.01.T01:CDS 50.0%
AGCAGTTGCAAGCAGCCTTG+CGG - Chr4:18665416-18665435 None:intergenic 55.0%
GGTTAGTGCACCAATGGCAG+AGG + Chr4:18665257-18665276 MsG0480019340.01.T01:CDS 55.0%
!! CTTAACGGTGCCGCCAACAA+CGG + Chr4:18665384-18665403 MsG0480019340.01.T01:CDS 55.0%
AACCAAGCTGCTGCTCTCCC+TGG + Chr4:18665474-18665493 MsG0480019340.01.T01:CDS 60.0%
CCACCACAGCATGCTGCAGA+TGG - Chr4:18665354-18665373 None:intergenic 60.0%
CCATCTGCAGCATGCTGTGG+TGG + Chr4:18665351-18665370 MsG0480019340.01.T01:CDS 60.0%
GCAGAGGCAGCAGTCACATG+TGG + Chr4:18665273-18665292 MsG0480019340.01.T01:CDS 60.0%
GCAGTTGCAAGCAGCCTTGC+GGG - Chr4:18665415-18665434 None:intergenic 60.0%
GCTGCTCTCCCTGGCAAATG+TGG + Chr4:18665483-18665502 MsG0480019340.01.T01:CDS 60.0%
GGTCCATCTGCAGCATGCTG+TGG + Chr4:18665348-18665367 MsG0480019340.01.T01:CDS 60.0%
GTGACTGCTGCCTCTGCCAT+TGG - Chr4:18665270-18665289 None:intergenic 60.0%
! CAGCATGCTGTGGTGGAGTG+AGG + Chr4:18665358-18665377 MsG0480019340.01.T01:CDS 60.0%
TGCAAGCAGCCTTGCGGGCA+GGG - Chr4:18665410-18665429 None:intergenic 65.0%
TGCCAGGGAGAGCAGCAGCT+TGG - Chr4:18665479-18665498 None:intergenic 65.0%
TTGCAAGCAGCCTTGCGGGC+AGG - Chr4:18665411-18665430 None:intergenic 65.0%
CAACAACGGCCCTGCCCGCA+AGG + Chr4:18665398-18665417 MsG0480019340.01.T01:CDS 70.0%
CTTGCGGGCAGGGCCGTTGT+TGG - Chr4:18665400-18665419 None:intergenic 70.0%
GCGGGCAGGGCCGTTGTTGG+CGG - Chr4:18665397-18665416 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 18665207 18665575 18665207 ID=MsG0480019340.01;Name=MsG0480019340.01
Chr4 mRNA 18665207 18665575 18665207 ID=MsG0480019340.01.T01;Parent=MsG0480019340.01;Name=MsG0480019340.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|122
Chr4 exon 18665207 18665575 18665207 ID=MsG0480019340.01.T01:exon:10879;Parent=MsG0480019340.01.T01
Chr4 CDS 18665207 18665575 18665207 ID=MsG0480019340.01.T01:cds;Parent=MsG0480019340.01.T01
Gene Sequence

>MsG0480019340.01.T01

ATGGCAAGCATGAGAATTGCATTTGTGGCTGCAATTATGTGCATGATGGTGGTTAGTGCACCAATGGCAGAGGCAGCAGTCACATGTGGACAAGTAGTAGGTTTCCTTACTCCATGCATTACTTATCTTCAAGGTGGTGCTGGTCCATCTGCAGCATGCTGTGGTGGAGTGAGGAGACTTAACGGTGCCGCCAACAACGGCCCTGCCCGCAAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTTCCATTGCTCGTTTGAATAACAACCAAGCTGCTGCTCTCCCTGGCAAATGTGGTGTTAACATTCCCTACAAGTTTAGCACTTCTACTAACTGTAATAGGTATGTTTTATATCTCACTCCTTAA

Protein sequence

>MsG0480019340.01.T01

MASMRIAFVAAIMCMMVVSAPMAEAAVTCGQVVGFLTPCITYLQGGAGPSAACCGGVRRLNGAANNGPARKAACNCLKSAAGSIARLNNNQAAALPGKCGVNIPYKFSTSTNCNRYVLYLTP*