AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043994.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043994.01.T01 MTR_8g045695 91.667 96 8 0 23 118 61 156 2.46e-60 182
MsG0880043994.01.T01 MTR_8g045735 92.553 94 7 0 23 116 61 154 5.34e-60 181
MsG0880043994.01.T01 MTR_8g045665 85.417 96 14 0 23 118 61 156 7.67e-56 171
MsG0880043994.01.T01 MTR_8g045560 83.505 97 16 0 22 118 60 156 5.30e-54 166
MsG0880043994.01.T01 MTR_8g045640 83.505 97 16 0 22 118 60 156 6.75e-54 166
MsG0880043994.01.T01 MTR_8g045520 71.134 97 27 1 23 118 61 157 8.14e-45 143
MsG0880043994.01.T01 MTR_8g045570 72.826 92 24 1 23 113 61 152 4.84e-44 141
MsG0880043994.01.T01 MTR_8g045555 69.565 92 27 1 23 113 61 152 5.48e-43 138
MsG0880043994.01.T01 MTR_8g045400 67.010 97 30 2 23 118 61 156 5.26e-42 135
MsG0880043994.01.T01 MTR_8g045490 68.041 97 29 2 23 118 61 156 1.08e-41 135
MsG0880043994.01.T01 MTR_8g045300 65.625 96 32 1 23 117 61 156 2.37e-41 134
MsG0880043994.01.T01 MTR_1g030810 41.758 91 50 2 25 112 62 152 4.05e-19 77.8
MsG0880043994.01.T01 MTR_1g030820 38.462 91 53 2 25 112 62 152 1.64e-17 73.6
MsG0880043994.01.T01 MTR_8g012550 37.363 91 55 2 26 115 66 155 5.47e-17 72.4
MsG0880043994.01.T01 MTR_1g031640 44.444 90 43 4 25 113 64 147 1.36e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043994.01.T01 AT1G70870 45.263 95 49 3 20 112 45 138 1.23e-18 76.3
MsG0880043994.01.T01 AT5G28010 35.106 94 59 2 23 114 73 166 4.11e-14 65.1
MsG0880043994.01.T01 AT5G28010 35.106 94 59 2 23 114 73 166 4.11e-14 65.1
MsG0880043994.01.T01 AT1G70850 37.234 94 57 2 23 114 223 316 1.66e-13 65.5
MsG0880043994.01.T01 AT1G70850 37.234 94 57 2 23 114 223 316 1.66e-13 65.5
MsG0880043994.01.T01 AT1G24020 38.372 86 51 2 28 112 67 151 2.73e-13 62.8
MsG0880043994.01.T01 AT1G24020 38.372 86 51 2 28 112 67 151 2.73e-13 62.8
MsG0880043994.01.T01 AT5G28000 35.714 98 60 3 20 114 67 164 7.40e-13 61.6
MsG0880043994.01.T01 AT1G70840 34.043 94 60 2 23 114 78 171 1.03e-12 61.6
MsG0880043994.01.T01 AT1G23120 35.106 94 58 3 19 111 54 145 1.05e-12 60.8
MsG0880043994.01.T01 AT1G70830 35.106 94 59 2 23 114 80 173 1.67e-12 60.8
MsG0880043994.01.T01 AT1G70830 34.737 95 60 2 23 115 80 174 1.81e-12 61.2
MsG0880043994.01.T01 AT1G23120 35.484 93 57 3 20 111 105 195 1.91e-12 61.2
MsG0880043994.01.T01 AT1G70890 35.106 94 59 2 23 114 65 158 2.77e-12 60.1
MsG0880043994.01.T01 AT4G23670 35.556 90 56 2 25 113 63 151 5.09e-11 56.6

Find 30 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATAACTTTCAAGTTGTT+TGG 0.327904 8:-34031094 MsG0880043994.01.T01:CDS
ATGGAAGTTCTGCTGTTAGA+TGG 0.407851 8:-34031012 MsG0880043994.01.T01:CDS
AATTCAAGTTCGAGTTCAAC+TGG 0.415188 8:-34032077 MsG0880043994.01.T01:CDS
TGGAGATATTGAAAACTATA+AGG 0.439418 8:-34031071 MsG0880043994.01.T01:CDS
AGGAAATCCATCCATCCATT+TGG 0.440781 8:+34030950 None:intergenic
CATGAGTTGCTTGGTATAGC+AGG 0.455659 8:+34032105 None:intergenic
AATTCCCCACATGAGTTGCT+TGG 0.459855 8:+34032096 None:intergenic
AAAAGCACTAGAGATATTGA+TGG 0.477335 8:-34030923 MsG0880043994.01.T01:CDS
ATAACTTTCAAGTTGTTTGG+TGG 0.479625 8:-34031091 MsG0880043994.01.T01:CDS
CGAGTTCAACTGGATGGTTG+AGG 0.483822 8:-34032067 MsG0880043994.01.T01:CDS
GAGTTCAACTGGATGGTTGA+GGG 0.500056 8:-34032066 MsG0880043994.01.T01:intron
TCATCTTGTCAAAGGGGAAA+AGG 0.504175 8:-34030900 MsG0880043994.01.T01:CDS
TTGATGGTCATCTTGTCAAA+GGG 0.506740 8:-34030907 MsG0880043994.01.T01:CDS
CAAGTTCGAGTTCAACTGGA+TGG 0.509876 8:-34032073 MsG0880043994.01.T01:CDS
GAGGATATCGATACTCCAAA+TGG 0.515854 8:-34030965 MsG0880043994.01.T01:CDS
TAACATAACAACAGATGGTA+AGG 0.518649 8:-34031164 MsG0880043994.01.T01:intron
CGATACTCCAAATGGATGGA+TGG 0.534738 8:-34030957 MsG0880043994.01.T01:CDS
ATTGATGGTCATCTTGTCAA+AGG 0.543582 8:-34030908 MsG0880043994.01.T01:CDS
GAAGTGCTTAACAAGGCTGA+TGG 0.580204 8:-34031031 MsG0880043994.01.T01:CDS
CTATACCAAGCAACTCATGT+GGG 0.591208 8:-34032101 MsG0880043994.01.T01:CDS
ATATCGATACTCCAAATGGA+TGG 0.593139 8:-34030961 MsG0880043994.01.T01:CDS
AGGTACACACATGCAATGAG+AGG 0.626711 8:-34031144 MsG0880043994.01.T01:CDS
TTGCTTGGTATAGCAGGTGT+GGG 0.632870 8:+34032111 None:intergenic
GCTATACCAAGCAACTCATG+TGG 0.632973 8:-34032102 MsG0880043994.01.T01:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8:-34031038 MsG0880043994.01.T01:CDS
TGATGGTCATCTTGTCAAAG+GGG 0.652198 8:-34030906 MsG0880043994.01.T01:CDS
GTTGCTTGGTATAGCAGGTG+TGG 0.657481 8:+34032110 None:intergenic
TATACCAAGCAACTCATGTG+GGG 0.680077 8:-34032100 MsG0880043994.01.T01:CDS
TGATTATGAGAAGAACAATG+AGG 0.681814 8:-34030984 MsG0880043994.01.T01:CDS
GGTACACACATGCAATGAGA+GGG 0.691358 8:-34031143 MsG0880043994.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGTTTTTTATTGTTTT+TGG - Chr8:34031806-34031825 MsG0880043994.01.T01:intron 10.0%
!!! TAAATTTTCACTTTTTGTTT+TGG + Chr8:34031334-34031353 None:intergenic 15.0%
!!! TAAGATAAAGTTTTTACTTT+TGG - Chr8:34031721-34031740 MsG0880043994.01.T01:intron 15.0%
!!! TATTAAAGTCAAACTTTTAT+CGG - Chr8:34031459-34031478 MsG0880043994.01.T01:intron 15.0%
!! AATCAAATCAAATTGTGTAA+TGG + Chr8:34031278-34031297 None:intergenic 20.0%
!! ATCAAAAATCAAATATCACA+AGG + Chr8:34031430-34031449 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG + Chr8:34031557-34031576 None:intergenic 20.0%
!!! ATTATTGTATTTTCTCTCTT+TGG + Chr8:34031245-34031264 None:intergenic 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG - Chr8:34031582-34031601 MsG0880043994.01.T01:intron 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG - Chr8:34031562-34031581 MsG0880043994.01.T01:intron 20.0%
!!! TTATTGTATTTTCTCTCTTT+GGG + Chr8:34031244-34031263 None:intergenic 20.0%
! AATGCTAACAAATGTTCTTA+AGG + Chr8:34031761-34031780 None:intergenic 25.0%
! ACAATATCTCTTGGAATTTA+CGG + Chr8:34031382-34031401 None:intergenic 25.0%
! ATAAAATTGCAGAATGATAG+AGG - Chr8:34031108-34031127 MsG0880043994.01.T01:CDS 25.0%
! ATAGAGGGAAATTATTGTTA+AGG - Chr8:34031124-34031143 MsG0880043994.01.T01:CDS 25.0%
! TAAAATTGCAGAATGATAGA+GGG - Chr8:34031109-34031128 MsG0880043994.01.T01:CDS 25.0%
! TGGAGATATTGAAAACTATA+AGG - Chr8:34031935-34031954 MsG0880043994.01.T01:intron 25.0%
!!! AAGATAACTTTCAAGTTGTT+TGG - Chr8:34031912-34031931 MsG0880043994.01.T01:intron 25.0%
!!! AATTTTTCGATCTAGTGTAA+TGG - Chr8:34030978-34030997 MsG0880043994.01.T01:CDS 25.0%
!!! ATTTTACTAATGAAGAGTAC+AGG - Chr8:34031205-34031224 MsG0880043994.01.T01:intron 25.0%
!!! ATTTTCACTTTTTGTTTTGG+TGG + Chr8:34031331-34031350 None:intergenic 25.0%
!!! CTGTTTAGGTTATTTTGATT+AGG + Chr8:34031305-34031324 None:intergenic 25.0%
!!! GTAATGGATAAGTAATTACA+TGG - Chr8:34030994-34031013 MsG0880043994.01.T01:CDS 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG - Chr8:34031435-34031454 MsG0880043994.01.T01:intron 25.0%
CTTAGGGTTAATAAAACGTA+TGG - Chr8:34031162-34031181 MsG0880043994.01.T01:intron 30.0%
GCTACAAAGTTAATCTCTTA+GGG - Chr8:34031146-34031165 MsG0880043994.01.T01:CDS 30.0%
TAACATAACAACAGATGGTA+AGG - Chr8:34031842-34031861 MsG0880043994.01.T01:intron 30.0%
TAATTACATGGCACATATCT+AGG - Chr8:34031006-34031025 MsG0880043994.01.T01:CDS 30.0%
TGATTATGAGAAGAACAATG+AGG - Chr8:34032022-34032041 MsG0880043994.01.T01:intron 30.0%
! GTTTGACTTTAATAACCACA+AGG + Chr8:34031453-34031472 None:intergenic 30.0%
! TGATTTTAAACTATCCTGTG+AGG - Chr8:34031058-34031077 MsG0880043994.01.T01:CDS 30.0%
!! AAAAGCACTAGAGATATTGA+TGG - Chr8:34032083-34032102 MsG0880043994.01.T01:CDS 30.0%
!! ATAACTTTCAAGTTGTTTGG+TGG - Chr8:34031915-34031934 MsG0880043994.01.T01:intron 30.0%
AATCCTTAACTAGTGCTCTA+AGG + Chr8:34031624-34031643 None:intergenic 35.0%
ACCATCTGTTGTTATGTTAG+AGG + Chr8:34031841-34031860 None:intergenic 35.0%
ATATCGATACTCCAAATGGA+TGG - Chr8:34032045-34032064 MsG0880043994.01.T01:intron 35.0%
ATCCTTAACTAGTGCTCTAA+GGG + Chr8:34031623-34031642 None:intergenic 35.0%
CCAAGAGATATTGTGCTTTA+TGG - Chr8:34031388-34031407 MsG0880043994.01.T01:intron 35.0%
CCATAAAGCACAATATCTCT+TGG + Chr8:34031391-34031410 None:intergenic 35.0%
GATTAACTTTGTAACCTCAC+AGG + Chr8:34031075-34031094 None:intergenic 35.0%
GGCTACAAAGTTAATCTCTT+AGG - Chr8:34031145-34031164 MsG0880043994.01.T01:CDS 35.0%
TGGCACATATCTAGGTAAAA+CGG - Chr8:34031014-34031033 MsG0880043994.01.T01:CDS 35.0%
! AATTCAAGTTCGAGTTCAAC+TGG - Chr8:34030929-34030948 MsG0880043994.01.T01:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+AGG - Chr8:34032098-34032117 MsG0880043994.01.T01:CDS 35.0%
! GCATCGATATCCTTATCATT+AGG - Chr8:34031544-34031563 MsG0880043994.01.T01:intron 35.0%
! GTTTGCTACGATAGCAAAAA+GGG + Chr8:34031360-34031379 None:intergenic 35.0%
! TATCTCTAGTGCTTTTGCTA+AGG + Chr8:34032079-34032098 None:intergenic 35.0%
! TTGATGGTCATCTTGTCAAA+GGG - Chr8:34032099-34032118 MsG0880043994.01.T01:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - Chr8:34031968-34031987 MsG0880043994.01.T01:intron 35.0%
AGGAAATCCATCCATCCATT+TGG + Chr8:34032059-34032078 None:intergenic 40.0%
ATGGAAGTTCTGCTGTTAGA+TGG - Chr8:34031994-34032013 MsG0880043994.01.T01:intron 40.0%
CTATACCAAGCAACTCATGT+GGG - Chr8:34030905-34030924 MsG0880043994.01.T01:CDS 40.0%
GAGGATATCGATACTCCAAA+TGG - Chr8:34032041-34032060 MsG0880043994.01.T01:intron 40.0%
GCCTCTAACATAACAACAGA+TGG - Chr8:34031837-34031856 MsG0880043994.01.T01:intron 40.0%
TATACCAAGCAACTCATGTG+GGG - Chr8:34030906-34030925 MsG0880043994.01.T01:CDS 40.0%
TCATCTTGTCAAAGGGGAAA+AGG - Chr8:34032106-34032125 MsG0880043994.01.T01:CDS 40.0%
TGATGGTCATCTTGTCAAAG+GGG - Chr8:34032100-34032119 MsG0880043994.01.T01:CDS 40.0%
! TTCCCTTAGAGCACTAGTTA+AGG - Chr8:34031618-34031637 MsG0880043994.01.T01:intron 40.0%
!! GGTTTGCTACGATAGCAAAA+AGG + Chr8:34031361-34031380 None:intergenic 40.0%
!!! TGTTTTGGTGGCAACTGTTT+AGG + Chr8:34031319-34031338 None:intergenic 40.0%
AATTCCCCACATGAGTTGCT+TGG + Chr8:34030913-34030932 None:intergenic 45.0%
AGGTACACACATGCAATGAG+AGG - Chr8:34031862-34031881 MsG0880043994.01.T01:intron 45.0%
CATGAGTTGCTTGGTATAGC+AGG + Chr8:34030904-34030923 None:intergenic 45.0%
CGATACTCCAAATGGATGGA+TGG - Chr8:34032049-34032068 MsG0880043994.01.T01:intron 45.0%
GAGTTCAACTGGATGGTTGA+GGG - Chr8:34030940-34030959 MsG0880043994.01.T01:CDS 45.0%
GCTATACCAAGCAACTCATG+TGG - Chr8:34030904-34030923 MsG0880043994.01.T01:CDS 45.0%
GGTACACACATGCAATGAGA+GGG - Chr8:34031863-34031882 MsG0880043994.01.T01:intron 45.0%
TTGCTTGGTATAGCAGGTGT+GGG + Chr8:34030898-34030917 None:intergenic 45.0%
! CAAGTTCGAGTTCAACTGGA+TGG - Chr8:34030933-34030952 MsG0880043994.01.T01:CDS 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - Chr8:34031975-34031994 MsG0880043994.01.T01:intron 45.0%
!! GTTGAGGGTTAGTCTTGACT+TGG - Chr8:34030955-34030974 MsG0880043994.01.T01:CDS 45.0%
!! TTAAAGTAATATTAAAAATA+AGG + Chr8:34031785-34031804 None:intergenic 5.0%
CGAGTTCAACTGGATGGTTG+AGG - Chr8:34030939-34030958 MsG0880043994.01.T01:CDS 50.0%
GTTGCTTGGTATAGCAGGTG+TGG + Chr8:34030899-34030918 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 34030889 34032139 34030889 ID=MsG0880043994.01;Name=MsG0880043994.01
Chr8 mRNA 34030889 34032139 34030889 ID=MsG0880043994.01.T01;Parent=MsG0880043994.01;Name=MsG0880043994.01.T01;_AED=0.50;_eAED=0.62;_QI=0|0|0|1|1|1|2|0|118
Chr8 exon 34032067 34032139 34032067 ID=MsG0880043994.01.T01:exon:10336;Parent=MsG0880043994.01.T01
Chr8 exon 34030889 34031172 34030889 ID=MsG0880043994.01.T01:exon:10335;Parent=MsG0880043994.01.T01
Chr8 CDS 34032067 34032139 34032067 ID=MsG0880043994.01.T01:cds;Parent=MsG0880043994.01.T01
Chr8 CDS 34030889 34031172 34030889 ID=MsG0880043994.01.T01:cds;Parent=MsG0880043994.01.T01
Gene Sequence

>MsG0880043994.01.T01

ATGGTTCCCACACCTGCTATACCAAGCAACTCATGTGGGGAATTCAAGTTCGAGTTCAACTGGATGGTTGAGGATGGTAAGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAAAACAAAAAGATAACTTTCAAGTTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATATCGATACTCCAAATGGATGGATGGATTTCCTTAGCAAAAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MsG0880043994.01.T01

MVPTPAIPSNSCGEFKFEFNWMVEDGKVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGSSAVRWTIDYEKNNEDIDTPNGWMDFLSKSTRDIDGHLVKGEKVAL*