AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046901.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046901.01.T01 MTR_4g116840 97.260 73 2 0 20 92 19 91 7.24e-46 157
MsG0880046901.01.T01 MTR_2g449780 77.333 75 17 0 18 92 19 93 5.36e-37 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046901.01.T01 AT5G35180 80.597 67 13 0 26 92 61 127 6.49e-36 128
MsG0880046901.01.T01 AT5G35180 80.597 67 13 0 26 92 61 127 1.14e-35 128
MsG0880046901.01.T01 AT5G35180 80.597 67 13 0 26 92 61 127 1.15e-35 128
MsG0880046901.01.T01 AT5G35180 80.597 67 13 0 26 92 61 127 1.19e-35 128
MsG0880046901.01.T01 AT5G35180 80.597 67 13 0 26 92 61 127 1.22e-35 128

Find 43 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAATATCATCAATTATT+TGG 0.143072 8:-79677402 None:intergenic
TTTCTTGTGGGACCCACTTT+TGG 0.271971 8:-79677368 None:intergenic
AACATACAATCAGTTTAGTT+TGG 0.334820 8:-79678335 None:intergenic
CCAACAACTCCCTTCCTAAT+CGG 0.370291 8:-79678229 None:intergenic
CCTATGTGCAGAAACCGATT+AGG 0.400153 8:+79678215 MsG0880046901.01.T01:intron
CTCACTATCTTCACCAAAAG+TGG 0.430020 8:+79677355 MsG0880046901.01.T01:five_prime_UTR
CCGATTAGGAAGGGAGTTGT+TGG 0.431236 8:+79678229 MsG0880046901.01.T01:CDS
TCGTCGGAGTTGAGCAAATC+GGG 0.435995 8:+79677541 MsG0880046901.01.T01:CDS
AGCTCCTCCACCATTAGTGT+GGG 0.439748 8:-79678253 None:intergenic
AGTTGTTGGCCCCACACTAA+TGG 0.454665 8:+79678243 MsG0880046901.01.T01:CDS
ATGAGGATCGCGCTTGTACA+TGG 0.467319 8:-79677677 None:intergenic
TTAGTAGAACATACAATGGC+AGG 0.484334 8:-79677706 None:intergenic
GTCGTCGGAGTTGAGCAAAT+CGG 0.492783 8:+79677540 MsG0880046901.01.T01:CDS
GCGATGGACGCAATTGCAGC+AGG 0.494054 8:+79677511 MsG0880046901.01.T01:exon
CACTTAGGAGTCAATTCCAT+TGG 0.500435 8:+79677604 MsG0880046901.01.T01:CDS
GAAGAATAAGTGATTAAGTG+TGG 0.502008 8:+79677476 MsG0880046901.01.T01:five_prime_UTR
CGGAGAGAAGTAGCTTCGGT+TGG 0.520719 8:+79677575 MsG0880046901.01.T01:CDS
TAGCTCCTCCACCATTAGTG+TGG 0.521419 8:-79678254 None:intergenic
ACACTAATGGTGGAGGAGCT+AGG 0.526606 8:+79678256 MsG0880046901.01.T01:CDS
GTGGGTGTTCGTGCGTGCGA+TGG 0.534813 8:+79677495 MsG0880046901.01.T01:exon
GGATCGGAGAGAAGTAGCTT+CGG 0.552381 8:+79677571 MsG0880046901.01.T01:CDS
GTCGCCGAAAAGTCAACGAT+GGG 0.554061 8:+79678278 MsG0880046901.01.T01:CDS
GGAGAGAAGTAGCTTCGGTT+GGG 0.560979 8:+79677576 MsG0880046901.01.T01:CDS
ATGACAATACTGACGACCAA+TGG 0.563811 8:-79677620 None:intergenic
GAAGCAGAAGCAGAAGCAGA+GGG 0.565391 8:+79677445 MsG0880046901.01.T01:five_prime_UTR
TTGAGCAAATCGGGTAGCAA+AGG 0.567129 8:+79677550 MsG0880046901.01.T01:CDS
TTCGGTTGGGTATATCACTT+AGG 0.568294 8:+79677589 MsG0880046901.01.T01:CDS
AGAAGCAGAAGCAGAAGCAG+AGG 0.568644 8:+79677444 MsG0880046901.01.T01:five_prime_UTR
TGCAGCAGGTTCATCGTCGT+CGG 0.578050 8:+79677525 MsG0880046901.01.T01:CDS
AAGAATAAGTGATTAAGTGT+GGG 0.579947 8:+79677477 MsG0880046901.01.T01:five_prime_UTR
GTGAGGTCACTCACTCCCAC+TGG 0.591902 8:-79677337 None:intergenic
TGGCCCCACACTAATGGTGG+AGG 0.605263 8:+79678249 MsG0880046901.01.T01:CDS
TGTGCAGAAACCGATTAGGA+AGG 0.609190 8:+79678219 MsG0880046901.01.T01:intron
TAGTAGAACATACAATGGCA+GGG 0.609987 8:-79677705 None:intergenic
TCACTATCTTCACCAAAAGT+GGG 0.615102 8:+79677356 MsG0880046901.01.T01:five_prime_UTR
GTCAGCTCAACCTCAACCAG+TGG 0.621631 8:+79677321 None:intergenic
GTGCAGAAACCGATTAGGAA+GGG 0.625948 8:+79678220 MsG0880046901.01.T01:intron
TGTTGGCCCCACACTAATGG+TGG 0.633247 8:+79678246 MsG0880046901.01.T01:CDS
CAAATCGGGTAGCAAAGGAT+CGG 0.634849 8:+79677555 MsG0880046901.01.T01:CDS
GCTCCTCCACCATTAGTGTG+GGG 0.635931 8:-79678252 None:intergenic
GGTCGCCGAAAAGTCAACGA+TGG 0.641439 8:+79678277 MsG0880046901.01.T01:CDS
TCAGCTCAACCTCAACCAGT+GGG 0.681789 8:+79677322 None:intergenic
TCGCCGAAAAGTCAACGATG+GGG 0.736973 8:+79678279 MsG0880046901.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTGAATATCATCAATTATT+TGG - Chr8:79677405-79677424 None:intergenic 20.0%
!!! AAATTAAAGCATTAATCAGT+GGG - Chr8:79677833-79677852 None:intergenic 20.0%
!!! GCTTTATTTATTTTTCTTGT+GGG - Chr8:79677383-79677402 None:intergenic 20.0%
! AAGAATAAGTGATTAAGTGT+GGG + Chr8:79677477-79677496 MsG0880046901.01.T01:five_prime_UTR 25.0%
! AATGTAATTAAGAGTAGAGT+AGG - Chr8:79677743-79677762 None:intergenic 25.0%
! TGAATTAGTAGAACATACAA+TGG - Chr8:79677713-79677732 None:intergenic 25.0%
!! AATTAAAGCATTAATCAGTG+GGG - Chr8:79677832-79677851 None:intergenic 25.0%
!! GAAATTAAAGCATTAATCAG+TGG - Chr8:79677834-79677853 None:intergenic 25.0%
!!! ATCTGAAACAAACACTTTTT+TGG - Chr8:79677781-79677800 None:intergenic 25.0%
!!! GGCTTTATTTATTTTTCTTG+TGG - Chr8:79677384-79677403 None:intergenic 25.0%
AAATACTTACTGAGAAGTAG+GGG + Chr8:79677936-79677955 MsG0880046901.01.T01:intron 30.0%
ACAAATACTTACTGAGAAGT+AGG + Chr8:79677934-79677953 MsG0880046901.01.T01:intron 30.0%
CAAATACTTACTGAGAAGTA+GGG + Chr8:79677935-79677954 MsG0880046901.01.T01:intron 30.0%
GAAGAATAAGTGATTAAGTG+TGG + Chr8:79677476-79677495 MsG0880046901.01.T01:five_prime_UTR 30.0%
TCTCAGTAAGTATTTGTATG+TGG - Chr8:79677931-79677950 None:intergenic 30.0%
TTTATCATCAAAACGCTTGT+CGG + Chr8:79677878-79677897 MsG0880046901.01.T01:intron 30.0%
CAAATCTCTCTTTAGTTGAC+TGG + Chr8:79678093-79678112 MsG0880046901.01.T01:intron 35.0%
CAGTCAACTAAAGAGAGATT+TGG - Chr8:79678095-79678114 None:intergenic 35.0%
TAGTAGAACATACAATGGCA+GGG - Chr8:79677708-79677727 None:intergenic 35.0%
TCACTATCTTCACCAAAAGT+GGG + Chr8:79677356-79677375 MsG0880046901.01.T01:five_prime_UTR 35.0%
TTAATCAGTGGGGTAATTAG+AGG - Chr8:79677822-79677841 None:intergenic 35.0%
TTAGTAGAACATACAATGGC+AGG - Chr8:79677709-79677728 None:intergenic 35.0%
!! CTTCGTTTTCTTTTGATTCG+AGG + Chr8:79677643-79677662 MsG0880046901.01.T01:CDS 35.0%
ATGACAATACTGACGACCAA+TGG - Chr8:79677623-79677642 None:intergenic 40.0%
CACTTAGGAGTCAATTCCAT+TGG + Chr8:79677604-79677623 MsG0880046901.01.T01:CDS 40.0%
CTCACTATCTTCACCAAAAG+TGG + Chr8:79677355-79677374 MsG0880046901.01.T01:five_prime_UTR 40.0%
TTCGGTTGGGTATATCACTT+AGG + Chr8:79677589-79677608 MsG0880046901.01.T01:CDS 40.0%
! CACTTTTGGTGAAGATAGTG+AGG - Chr8:79677357-79677376 None:intergenic 40.0%
!!! TTACCCCATCGTTGACTTTT+CGG - Chr8:79678285-79678304 None:intergenic 40.0%
CAAATCGGGTAGCAAAGGAT+CGG + Chr8:79677555-79677574 MsG0880046901.01.T01:CDS 45.0%
CCAACAACTCCCTTCCTAAT+CGG - Chr8:79678232-79678251 None:intergenic 45.0%
CCTATGTGCAGAAACCGATT+AGG + Chr8:79678215-79678234 MsG0880046901.01.T01:intron 45.0%
GCGTTGCGGATTTGAACTTA+CGG + Chr8:79677962-79677981 MsG0880046901.01.T01:intron 45.0%
GTGCAGAAACCGATTAGGAA+GGG + Chr8:79678220-79678239 MsG0880046901.01.T01:intron 45.0%
GTGGGGTAATTAGAGGTTAG+TGG - Chr8:79677815-79677834 None:intergenic 45.0%
TGTGCAGAAACCGATTAGGA+AGG + Chr8:79678219-79678238 MsG0880046901.01.T01:intron 45.0%
TTGAGCAAATCGGGTAGCAA+AGG + Chr8:79677550-79677569 MsG0880046901.01.T01:CDS 45.0%
! ACAATGGCAGGGTTTTCATG+AGG - Chr8:79677697-79677716 None:intergenic 45.0%
! CCTAATCGGTTTCTGCACAT+AGG - Chr8:79678218-79678237 None:intergenic 45.0%
! TTTCTTGTGGGACCCACTTT+TGG - Chr8:79677371-79677390 None:intergenic 45.0%
AGAAGCAGAAGCAGAAGCAG+AGG + Chr8:79677444-79677463 MsG0880046901.01.T01:five_prime_UTR 50.0%
AGAAGTAGGGGATTGCGTTG+CGG + Chr8:79677948-79677967 MsG0880046901.01.T01:intron 50.0%
AGCTCCTCCACCATTAGTGT+GGG - Chr8:79678256-79678275 None:intergenic 50.0%
AGTTGTTGGCCCCACACTAA+TGG + Chr8:79678243-79678262 MsG0880046901.01.T01:CDS 50.0%
ATGAGGATCGCGCTTGTACA+TGG - Chr8:79677680-79677699 None:intergenic 50.0%
CCGATTAGGAAGGGAGTTGT+TGG + Chr8:79678229-79678248 MsG0880046901.01.T01:CDS 50.0%
GAAGCAGAAGCAGAAGCAGA+GGG + Chr8:79677445-79677464 MsG0880046901.01.T01:five_prime_UTR 50.0%
GGAGAGAAGTAGCTTCGGTT+GGG + Chr8:79677576-79677595 MsG0880046901.01.T01:CDS 50.0%
GGATCGGAGAGAAGTAGCTT+CGG + Chr8:79677571-79677590 MsG0880046901.01.T01:CDS 50.0%
GTCGCCGAAAAGTCAACGAT+GGG + Chr8:79678278-79678297 MsG0880046901.01.T01:CDS 50.0%
GTCGTCGGAGTTGAGCAAAT+CGG + Chr8:79677540-79677559 MsG0880046901.01.T01:CDS 50.0%
TAGCTCCTCCACCATTAGTG+TGG - Chr8:79678257-79678276 None:intergenic 50.0%
TCGCCGAAAAGTCAACGATG+GGG + Chr8:79678279-79678298 MsG0880046901.01.T01:CDS 50.0%
TCGTCGGAGTTGAGCAAATC+GGG + Chr8:79677541-79677560 MsG0880046901.01.T01:CDS 50.0%
! ACACTAATGGTGGAGGAGCT+AGG + Chr8:79678256-79678275 MsG0880046901.01.T01:CDS 50.0%
CGGAGAGAAGTAGCTTCGGT+TGG + Chr8:79677575-79677594 MsG0880046901.01.T01:CDS 55.0%
GCTCCTCCACCATTAGTGTG+GGG - Chr8:79678255-79678274 None:intergenic 55.0%
GGTCGCCGAAAAGTCAACGA+TGG + Chr8:79678277-79678296 MsG0880046901.01.T01:CDS 55.0%
TGCAGCAGGTTCATCGTCGT+CGG + Chr8:79677525-79677544 MsG0880046901.01.T01:CDS 55.0%
TGTTGGCCCCACACTAATGG+TGG + Chr8:79678246-79678265 MsG0880046901.01.T01:CDS 55.0%
GCGATGGACGCAATTGCAGC+AGG + Chr8:79677511-79677530 MsG0880046901.01.T01:exon 60.0%
GTGAGGTCACTCACTCCCAC+TGG - Chr8:79677340-79677359 None:intergenic 60.0%
TGGCCCCACACTAATGGTGG+AGG + Chr8:79678249-79678268 MsG0880046901.01.T01:CDS 60.0%
GTGGGTGTTCGTGCGTGCGA+TGG + Chr8:79677495-79677514 MsG0880046901.01.T01:exon 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 79677335 79678348 79677335 ID=MsG0880046901.01;Name=MsG0880046901.01
Chr8 mRNA 79677335 79678348 79677335 ID=MsG0880046901.01.T01;Parent=MsG0880046901.01;Name=MsG0880046901.01.T01;_AED=0.50;_eAED=0.51;_QI=179|1|0.5|1|1|1|2|0|107
Chr8 exon 79677335 79677714 79677335 ID=MsG0880046901.01.T01:exon:34411;Parent=MsG0880046901.01.T01
Chr8 exon 79678226 79678348 79678226 ID=MsG0880046901.01.T01:exon:34412;Parent=MsG0880046901.01.T01
Chr8 five_prime_UTR 79677335 79677513 79677335 ID=MsG0880046901.01.T01:five_prime_utr;Parent=MsG0880046901.01.T01
Chr8 CDS 79677514 79677714 79677514 ID=MsG0880046901.01.T01:cds;Parent=MsG0880046901.01.T01
Chr8 CDS 79678226 79678348 79678226 ID=MsG0880046901.01.T01:cds;Parent=MsG0880046901.01.T01
Gene Sequence

>MsG0880046901.01.T01

ATGGACGCAATTGCAGCAGGTTCATCGTCGTCGGAGTTGAGCAAATCGGGTAGCAAAGGATCGGAGAGAAGTAGCTTCGGTTGGGTATATCACTTAGGAGTCAATTCCATTGGTCGTCAGTATTGTCATCTTCGTTTTCTTTTGATTCGAGGAAAGTATGTTTCCATGTACAAGCGCGATCCTCATGAAAACCCTGCCATTAAACCGATTAGGAAGGGAGTTGTTGGCCCCACACTAATGGTGGAGGAGCTAGGTCGCCGAAAAGTCAACGATGGGGTAAAATTCTTGCATACTATTCTCTTTGCATTTACCAAACTAAACTGA

Protein sequence

>MsG0880046901.01.T01

MDAIAAGSSSSELSKSGSKGSERSSFGWVYHLGVNSIGRQYCHLRFLLIRGKYVSMYKRDPHENPAIKPIRKGVVGPTLMVEELGRRKVNDGVKFLHTILFAFTKLN*