Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044676.01.T01 | GAU48218.1 | 97.368 | 152 | 4 | 0 | 1 | 152 | 1 | 152 | 9.83E-104 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044676.01.T01 | Q6NQE2 | 88.591 | 149 | 17 | 0 | 1 | 149 | 52 | 200 | 1.07E-94 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044676.01.T01 | A0A2Z6NVK5 | 97.368 | 152 | 4 | 0 | 1 | 152 | 1 | 152 | 4.70e-104 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001029.01 | MsG0880044676.01 | 0.801760 | 7.732585e-49 | 3.722873e-46 |
| MsG0180001513.01 | MsG0880044676.01 | 0.804445 | 2.153203e-49 | 1.110368e-46 |
| MsG0180003484.01 | MsG0880044676.01 | 0.816008 | 6.899207e-52 | 4.831590e-49 |
| MsG0380011702.01 | MsG0880044676.01 | 0.807885 | 4.062898e-50 | 2.289366e-47 |
| MsG0380013843.01 | MsG0880044676.01 | 0.816048 | 6.759842e-52 | 4.739342e-49 |
| MsG0680030613.01 | MsG0880044676.01 | 0.802787 | 4.752984e-49 | 2.349147e-46 |
| MsG0280009526.01 | MsG0880044676.01 | 0.801417 | 9.093851e-49 | 4.340550e-46 |
| MsG0780039912.01 | MsG0880044676.01 | 0.808315 | 3.290144e-50 | 1.875215e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044676.01.T01 | MTR_8g466030 | 98.684 | 152 | 2 | 0 | 1 | 152 | 52 | 203 | 4.49e-107 | 303 |
| MsG0880044676.01.T01 | MTR_2g081810 | 90.132 | 152 | 15 | 0 | 1 | 152 | 52 | 203 | 6.23e-100 | 285 |
| MsG0880044676.01.T01 | MTR_4g091900 | 81.879 | 149 | 27 | 0 | 1 | 149 | 41 | 189 | 4.28e-87 | 253 |
| MsG0880044676.01.T01 | MTR_4g091900 | 81.879 | 149 | 27 | 0 | 1 | 149 | 53 | 201 | 7.07e-87 | 253 |
| MsG0880044676.01.T01 | MTR_8g466080 | 93.277 | 119 | 8 | 0 | 34 | 152 | 1 | 119 | 2.68e-76 | 223 |
| MsG0880044676.01.T01 | MTR_5g070030 | 70.667 | 150 | 43 | 1 | 1 | 149 | 53 | 202 | 1.27e-74 | 221 |
| MsG0880044676.01.T01 | MTR_2g011080 | 70.470 | 149 | 44 | 0 | 1 | 149 | 1 | 149 | 1.47e-74 | 219 |
| MsG0880044676.01.T01 | MTR_2g011080 | 70.470 | 149 | 44 | 0 | 1 | 149 | 52 | 200 | 2.17e-74 | 221 |
| MsG0880044676.01.T01 | MTR_2g011080 | 70.470 | 149 | 44 | 0 | 1 | 149 | 47 | 195 | 2.67e-74 | 221 |
| MsG0880044676.01.T01 | MTR_4g069250 | 69.128 | 149 | 45 | 1 | 1 | 148 | 100 | 248 | 4.72e-70 | 212 |
| MsG0880044676.01.T01 | MTR_5g019620 | 63.158 | 152 | 55 | 1 | 1 | 151 | 101 | 252 | 2.21e-65 | 200 |
| MsG0880044676.01.T01 | MTR_4g091890 | 82.051 | 117 | 17 | 1 | 33 | 149 | 1 | 113 | 2.86e-64 | 192 |
| MsG0880044676.01.T01 | MTR_8g466110 | 65.600 | 125 | 15 | 4 | 1 | 124 | 1 | 98 | 5.33e-43 | 138 |
| MsG0880044676.01.T01 | MTR_8g466150 | 65.600 | 125 | 15 | 4 | 1 | 124 | 1 | 98 | 5.33e-43 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044676.01.T01 | AT4G27270 | 88.591 | 149 | 17 | 0 | 1 | 149 | 1 | 149 | 6.68e-96 | 273 |
| MsG0880044676.01.T01 | AT4G27270 | 88.591 | 149 | 17 | 0 | 1 | 149 | 41 | 189 | 1.02e-95 | 275 |
| MsG0880044676.01.T01 | AT4G27270 | 88.591 | 149 | 17 | 0 | 1 | 149 | 41 | 189 | 1.02e-95 | 275 |
| MsG0880044676.01.T01 | AT4G27270 | 88.591 | 149 | 17 | 0 | 1 | 149 | 52 | 200 | 1.09e-95 | 275 |
| MsG0880044676.01.T01 | AT5G54500 | 88.591 | 149 | 17 | 0 | 1 | 149 | 52 | 200 | 1.71e-95 | 275 |
| MsG0880044676.01.T01 | AT4G36750 | 68.212 | 151 | 47 | 1 | 1 | 150 | 122 | 272 | 6.04e-70 | 212 |
| MsG0880044676.01.T01 | AT5G58800 | 65.333 | 150 | 52 | 0 | 3 | 152 | 56 | 205 | 1.32e-68 | 206 |
| MsG0880044676.01.T01 | AT5G58800 | 65.333 | 150 | 52 | 0 | 3 | 152 | 56 | 205 | 1.32e-68 | 206 |
| MsG0880044676.01.T01 | AT5G54500 | 71.918 | 146 | 33 | 2 | 1 | 146 | 52 | 189 | 6.44e-68 | 206 |
Find 48 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGATTGGTACATCACTTT+TGG | 0.233841 | 8:-47135969 | None:intergenic |
| TTTGGATTTCCAACAAGATT+TGG | 0.260288 | 8:+47136028 | MsG0880044676.01.T01:CDS |
| TTGGCCACCGCCTTGGGAAC+TGG | 0.307650 | 8:-47136149 | None:intergenic |
| GGAATGCTATTTGTTCCAAT+TGG | 0.311832 | 8:+47136599 | MsG0880044676.01.T01:CDS |
| CACCTTCAGTTAGTTCATTC+GGG | 0.339232 | 8:-47135994 | None:intergenic |
| GTTCCAATTGGGTATACATT+TGG | 0.346928 | 8:+47136611 | MsG0880044676.01.T01:CDS |
| TCACCTTCAGTTAGTTCATT+CGG | 0.361037 | 8:-47135995 | None:intergenic |
| CTTGAGTTTCTTTGTGATTG+TGG | 0.375549 | 8:-47136765 | None:intergenic |
| AGTCTCTTGGCCACCGCCTT+GGG | 0.379897 | 8:-47136155 | None:intergenic |
| TGGAAAACACAACAGCTTGC+AGG | 0.385865 | 8:+47136100 | MsG0880044676.01.T01:CDS |
| GAATGCTATTTGTTCCAATT+GGG | 0.403516 | 8:+47136600 | MsG0880044676.01.T01:CDS |
| TGTTCCAACTCAAGCTTAGT+TGG | 0.407575 | 8:-47136716 | None:intergenic |
| TAGTCTCTTGGCCACCGCCT+TGG | 0.422756 | 8:-47136156 | None:intergenic |
| ACTGGTGCTGTAGAAGATTC+CGG | 0.424333 | 8:-47136131 | None:intergenic |
| AGTTCATTCGGGATAATGAT+TGG | 0.424986 | 8:-47135983 | None:intergenic |
| AAAGCCTTTCTAGATGCAAC+TGG | 0.427111 | 8:+47136070 | MsG0880044676.01.T01:CDS |
| GGTGGAAGTCCATATGGTTC+AGG | 0.428735 | 8:+47136665 | MsG0880044676.01.T01:CDS |
| GCCATCATTCCAAATCTTGT+TGG | 0.428775 | 8:-47136037 | None:intergenic |
| TCCAACAAGATTTGGAATGA+TGG | 0.429962 | 8:+47136036 | MsG0880044676.01.T01:CDS |
| TAGATGCAACTGGAGGTCTA+TGG | 0.451000 | 8:+47136080 | MsG0880044676.01.T01:CDS |
| GCTCACTGCTATTACTCAAT+TGG | 0.453952 | 8:+47136568 | MsG0880044676.01.T01:CDS |
| GTGAAAGGTGGAAGTCCATA+TGG | 0.471477 | 8:+47136659 | MsG0880044676.01.T01:CDS |
| GCACCAAATGTATACCCAAT+TGG | 0.472027 | 8:-47136614 | None:intergenic |
| GCATATGTTCCTGAACCATA+TGG | 0.493129 | 8:-47136674 | None:intergenic |
| ATTGGGTATACATTTGGTGC+TGG | 0.497066 | 8:+47136617 | MsG0880044676.01.T01:CDS |
| GCTCTTACGCTGTAGTCTCT+TGG | 0.513071 | 8:-47136168 | None:intergenic |
| ATTACTCAATTGGTGCATCA+TGG | 0.518479 | 8:+47136578 | MsG0880044676.01.T01:CDS |
| CAGCTTGCAGGGAAGCCTGC+CGG | 0.531216 | 8:+47136112 | MsG0880044676.01.T01:CDS |
| GGAAAACACAACAGCTTGCA+GGG | 0.531609 | 8:+47136101 | MsG0880044676.01.T01:CDS |
| ACCTCCAGTTGCATCTAGAA+AGG | 0.532710 | 8:-47136074 | None:intergenic |
| TATGGTTCAGGAACATATGC+TGG | 0.562306 | 8:+47136677 | MsG0880044676.01.T01:CDS |
| TTCTACAGCACCAGTTCCCA+AGG | 0.562862 | 8:+47136139 | MsG0880044676.01.T01:CDS |
| AGCACCAGTTCCCAAGGCGG+TGG | 0.563694 | 8:+47136145 | MsG0880044676.01.T01:CDS |
| TGGCAATATACATCCCTTGG+TGG | 0.572606 | 8:-47136745 | None:intergenic |
| ACAGCCAACTAAGCTTGAGT+TGG | 0.584176 | 8:+47136712 | MsG0880044676.01.T01:CDS |
| GAGATGGAAGAAGTGAAAGG+TGG | 0.588670 | 8:+47136647 | MsG0880044676.01.T01:CDS |
| TCAGGAACATATGCTGGTGA+TGG | 0.599049 | 8:+47136683 | MsG0880044676.01.T01:CDS |
| TGGAACAAGCATTCCACCAA+GGG | 0.605202 | 8:+47136732 | MsG0880044676.01.T01:CDS |
| ATCCCGAATGAACTAACTGA+AGG | 0.610161 | 8:+47135992 | MsG0880044676.01.T01:CDS |
| GTGCTGTAGAAGATTCCGGC+AGG | 0.618731 | 8:-47136127 | None:intergenic |
| TGGTGCTGGAATGTTTGAGA+TGG | 0.623605 | 8:+47136631 | MsG0880044676.01.T01:CDS |
| CACAATCACAAAGAAACTCA+AGG | 0.627466 | 8:+47136766 | MsG0880044676.01.T01:CDS |
| TTGTGGCAATATACATCCCT+TGG | 0.635784 | 8:-47136748 | None:intergenic |
| AATGAACTAACTGAAGGTGA+TGG | 0.639063 | 8:+47135998 | MsG0880044676.01.T01:CDS |
| GCCTTTCTAGATGCAACTGG+AGG | 0.664986 | 8:+47136073 | MsG0880044676.01.T01:CDS |
| TTGGAACAAGCATTCCACCA+AGG | 0.678388 | 8:+47136731 | MsG0880044676.01.T01:CDS |
| TTTGAGATGGAAGAAGTGAA+AGG | 0.685250 | 8:+47136644 | MsG0880044676.01.T01:CDS |
| TACAGCACCAGTTCCCAAGG+CGG | 0.707283 | 8:+47136142 | MsG0880044676.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAATTTGTAACATGTT+CGG | - | Chr8:47136413-47136432 | None:intergenic | 15.0% |
| !!! | ATATTTATGTTGAGAAATTT+TGG | + | Chr8:47136529-47136548 | MsG0880044676.01.T01:intron | 15.0% |
| !!! | GTTTTTAACAAAATTTACAA+TGG | - | Chr8:47136261-47136280 | None:intergenic | 15.0% |
| !! | CATGTTACAAATTTATAAAC+TGG | + | Chr8:47136415-47136434 | MsG0880044676.01.T01:intron | 20.0% |
| !! | GAAATCTAAATCTATTAGAT+CGG | + | Chr8:47136497-47136516 | MsG0880044676.01.T01:intron | 20.0% |
| !!! | GTGTGATTTAGTATATTTTT+TGG | - | Chr8:47136344-47136363 | None:intergenic | 20.0% |
| !!! | TCTTATTTTGATTTGATAGT+TGG | + | Chr8:47136469-47136488 | MsG0880044676.01.T01:intron | 20.0% |
| ATATTCTCTAGTGCTTTGTA+TGG | + | Chr8:47136203-47136222 | MsG0880044676.01.T01:intron | 30.0% | |
| TAGAGAATATGATCGAGTAA+AGG | - | Chr8:47136193-47136212 | None:intergenic | 30.0% | |
| TAGTGAGAATTACATAACCA+CGG | + | Chr8:47136231-47136250 | MsG0880044676.01.T01:intron | 30.0% | |
| TTTGGATTTCCAACAAGATT+TGG | + | Chr8:47136028-47136047 | MsG0880044676.01.T01:CDS | 30.0% | |
| ! | AATGATTGGTACATCACTTT+TGG | - | Chr8:47135972-47135991 | None:intergenic | 30.0% |
| ! | GAATGCTATTTGTTCCAATT+GGG | + | Chr8:47136600-47136619 | MsG0880044676.01.T01:CDS | 30.0% |
| ! | TTTTGGATTTAATCTATGCC+AGG | + | Chr8:47136546-47136565 | MsG0880044676.01.T01:intron | 30.0% |
| !! | ATGATTGGTACATCACTTTT+GGG | - | Chr8:47135971-47135990 | None:intergenic | 30.0% |
| !! | TTACAGTGTCATTGTGATTT+TGG | + | Chr8:47136303-47136322 | MsG0880044676.01.T01:intron | 30.0% |
| AATGAACTAACTGAAGGTGA+TGG | + | Chr8:47135998-47136017 | MsG0880044676.01.T01:CDS | 35.0% | |
| AGTTCATTCGGGATAATGAT+TGG | - | Chr8:47135986-47136005 | None:intergenic | 35.0% | |
| ATTACTCAATTGGTGCATCA+TGG | + | Chr8:47136578-47136597 | MsG0880044676.01.T01:CDS | 35.0% | |
| CACAATCACAAAGAAACTCA+AGG | + | Chr8:47136766-47136785 | MsG0880044676.01.T01:CDS | 35.0% | |
| CTTGAGTTTCTTTGTGATTG+TGG | - | Chr8:47136768-47136787 | None:intergenic | 35.0% | |
| GTTCCAATTGGGTATACATT+TGG | + | Chr8:47136611-47136630 | MsG0880044676.01.T01:CDS | 35.0% | |
| TCACCTTCAGTTAGTTCATT+CGG | - | Chr8:47135998-47136017 | None:intergenic | 35.0% | |
| ! | AAATTTACAATGGTTAGCCG+TGG | - | Chr8:47136251-47136270 | None:intergenic | 35.0% |
| ! | GGAATGCTATTTGTTCCAAT+TGG | + | Chr8:47136599-47136618 | MsG0880044676.01.T01:CDS | 35.0% |
| ! | TCCAACAAGATTTGGAATGA+TGG | + | Chr8:47136036-47136055 | MsG0880044676.01.T01:CDS | 35.0% |
| ! | TTTGAGATGGAAGAAGTGAA+AGG | + | Chr8:47136644-47136663 | MsG0880044676.01.T01:CDS | 35.0% |
| AAAGCCTTTCTAGATGCAAC+TGG | + | Chr8:47136070-47136089 | MsG0880044676.01.T01:CDS | 40.0% | |
| ATCCCGAATGAACTAACTGA+AGG | + | Chr8:47135992-47136011 | MsG0880044676.01.T01:CDS | 40.0% | |
| CACCTTCAGTTAGTTCATTC+GGG | - | Chr8:47135997-47136016 | None:intergenic | 40.0% | |
| GCACCAAATGTATACCCAAT+TGG | - | Chr8:47136617-47136636 | None:intergenic | 40.0% | |
| GCATATGTTCCTGAACCATA+TGG | - | Chr8:47136677-47136696 | None:intergenic | 40.0% | |
| GCCATCATTCCAAATCTTGT+TGG | - | Chr8:47136040-47136059 | None:intergenic | 40.0% | |
| GCTCACTGCTATTACTCAAT+TGG | + | Chr8:47136568-47136587 | MsG0880044676.01.T01:CDS | 40.0% | |
| TATGGTTCAGGAACATATGC+TGG | + | Chr8:47136677-47136696 | MsG0880044676.01.T01:CDS | 40.0% | |
| TGTTCCAACTCAAGCTTAGT+TGG | - | Chr8:47136719-47136738 | None:intergenic | 40.0% | |
| TTGTGGCAATATACATCCCT+TGG | - | Chr8:47136751-47136770 | None:intergenic | 40.0% | |
| ! | GGTCTAATCTTTTGACCAGA+TGG | - | Chr8:47136392-47136411 | None:intergenic | 40.0% |
| !! | ATTGGGTATACATTTGGTGC+TGG | + | Chr8:47136617-47136636 | MsG0880044676.01.T01:CDS | 40.0% |
| !! | ATTGGTACATCACTTTTGGG+TGG | - | Chr8:47135968-47135987 | None:intergenic | 40.0% |
| !!! | GAAGGTGATGGTTTTGTCTT+TGG | + | Chr8:47136010-47136029 | MsG0880044676.01.T01:CDS | 40.0% |
| ACAGCCAACTAAGCTTGAGT+TGG | + | Chr8:47136712-47136731 | MsG0880044676.01.T01:CDS | 45.0% | |
| ACCTCCAGTTGCATCTAGAA+AGG | - | Chr8:47136077-47136096 | None:intergenic | 45.0% | |
| GAGATGGAAGAAGTGAAAGG+TGG | + | Chr8:47136647-47136666 | MsG0880044676.01.T01:CDS | 45.0% | |
| GGAAAACACAACAGCTTGCA+GGG | + | Chr8:47136101-47136120 | MsG0880044676.01.T01:CDS | 45.0% | |
| GTGAAAGGTGGAAGTCCATA+TGG | + | Chr8:47136659-47136678 | MsG0880044676.01.T01:CDS | 45.0% | |
| TAGATGCAACTGGAGGTCTA+TGG | + | Chr8:47136080-47136099 | MsG0880044676.01.T01:CDS | 45.0% | |
| TCAGGAACATATGCTGGTGA+TGG | + | Chr8:47136683-47136702 | MsG0880044676.01.T01:CDS | 45.0% | |
| TGGAAAACACAACAGCTTGC+AGG | + | Chr8:47136100-47136119 | MsG0880044676.01.T01:CDS | 45.0% | |
| TGGAACAAGCATTCCACCAA+GGG | + | Chr8:47136732-47136751 | MsG0880044676.01.T01:CDS | 45.0% | |
| TGGCAATATACATCCCTTGG+TGG | - | Chr8:47136748-47136767 | None:intergenic | 45.0% | |
| TTGAGTAATAGCAGTGAGCC+TGG | - | Chr8:47136567-47136586 | None:intergenic | 45.0% | |
| TTGGAACAAGCATTCCACCA+AGG | + | Chr8:47136731-47136750 | MsG0880044676.01.T01:CDS | 45.0% | |
| ! | ACACATTTGACGCATCGACA+CGG | + | Chr8:47136360-47136379 | MsG0880044676.01.T01:intron | 45.0% |
| ! | TGGTGCTGGAATGTTTGAGA+TGG | + | Chr8:47136631-47136650 | MsG0880044676.01.T01:CDS | 45.0% |
| !! | ACTGGTGCTGTAGAAGATTC+CGG | - | Chr8:47136134-47136153 | None:intergenic | 45.0% |
| GCCTTTCTAGATGCAACTGG+AGG | + | Chr8:47136073-47136092 | MsG0880044676.01.T01:CDS | 50.0% | |
| GCTCTTACGCTGTAGTCTCT+TGG | - | Chr8:47136171-47136190 | None:intergenic | 50.0% | |
| GGTGGAAGTCCATATGGTTC+AGG | + | Chr8:47136665-47136684 | MsG0880044676.01.T01:CDS | 50.0% | |
| TCGACACGGTCTTTACCATC+TGG | + | Chr8:47136374-47136393 | MsG0880044676.01.T01:intron | 50.0% | |
| TTCTACAGCACCAGTTCCCA+AGG | + | Chr8:47136139-47136158 | MsG0880044676.01.T01:CDS | 50.0% | |
| GTGCTGTAGAAGATTCCGGC+AGG | - | Chr8:47136130-47136149 | None:intergenic | 55.0% | |
| TACAGCACCAGTTCCCAAGG+CGG | + | Chr8:47136142-47136161 | MsG0880044676.01.T01:CDS | 55.0% | |
| AGTCTCTTGGCCACCGCCTT+GGG | - | Chr8:47136158-47136177 | None:intergenic | 60.0% | |
| TAGTCTCTTGGCCACCGCCT+TGG | - | Chr8:47136159-47136178 | None:intergenic | 60.0% | |
| AGCACCAGTTCCCAAGGCGG+TGG | + | Chr8:47136145-47136164 | MsG0880044676.01.T01:CDS | 65.0% | |
| CAGCTTGCAGGGAAGCCTGC+CGG | + | Chr8:47136112-47136131 | MsG0880044676.01.T01:CDS | 65.0% | |
| TTGGCCACCGCCTTGGGAAC+TGG | - | Chr8:47136152-47136171 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 47135956 | 47136799 | 47135956 | ID=MsG0880044676.01;Name=MsG0880044676.01 |
| Chr8 | mRNA | 47135956 | 47136799 | 47135956 | ID=MsG0880044676.01.T01;Parent=MsG0880044676.01;Name=MsG0880044676.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|152 |
| Chr8 | exon | 47135956 | 47136182 | 47135956 | ID=MsG0880044676.01.T01:exon:19210;Parent=MsG0880044676.01.T01 |
| Chr8 | exon | 47136568 | 47136799 | 47136568 | ID=MsG0880044676.01.T01:exon:19211;Parent=MsG0880044676.01.T01 |
| Chr8 | CDS | 47135956 | 47136182 | 47135956 | ID=MsG0880044676.01.T01:cds;Parent=MsG0880044676.01.T01 |
| Chr8 | CDS | 47136568 | 47136799 | 47136568 | ID=MsG0880044676.01.T01:cds;Parent=MsG0880044676.01.T01 |
| Gene Sequence |
| Protein sequence |
