AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001029.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001029.01.T01 MTR_1g028970 97.479 119 3 0 1 119 1 119 1.62e-82 239
MsG0180001029.01.T01 MTR_1g028970 97.479 119 3 0 1 119 1 119 4.02e-82 239
MsG0180001029.01.T01 MTR_1g028970 97.479 119 3 0 1 119 1 119 4.11e-82 239
MsG0180001029.01.T01 MTR_1g028960 89.076 119 12 1 1 119 1 118 1.53e-71 213
MsG0180001029.01.T01 MTR_3g090530 67.568 111 36 0 9 119 7 117 1.31e-50 158
MsG0180001029.01.T01 MTR_3g090530 70.536 112 31 2 9 119 7 117 1.32e-49 157
MsG0180001029.01.T01 MTR_3g090530 67.568 111 36 0 9 119 7 117 2.61e-49 156
MsG0180001029.01.T01 MTR_1g075710 38.095 84 52 0 36 119 19 102 3.17e-15 68.6
MsG0180001029.01.T01 MTR_1g041495 39.286 84 51 0 36 119 19 102 1.32e-14 67.0
MsG0180001029.01.T01 MTR_1g041495 39.286 84 51 0 36 119 19 102 1.62e-14 67.0
MsG0180001029.01.T01 MTR_3g090530 63.043 46 17 0 74 119 7 52 3.39e-13 61.6
MsG0180001029.01.T01 MTR_3g090530 63.043 46 17 0 74 119 7 52 1.28e-12 60.8
MsG0180001029.01.T01 MTR_3g090530 63.043 46 17 0 74 119 7 52 1.76e-12 60.5
MsG0180001029.01.T01 MTR_1g075700 33.673 98 56 2 27 119 5 98 7.36e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001029.01.T01 AT1G21360 52.991 117 52 2 5 119 4 119 1.54e-35 120
MsG0180001029.01.T01 AT1G21360 52.991 117 52 2 5 119 4 119 1.98e-35 121
MsG0180001029.01.T01 AT3G21260 52.174 115 50 1 10 119 15 129 6.71e-32 112
MsG0180001029.01.T01 AT1G21360 54.386 57 26 0 63 119 1 57 1.85e-14 65.9
MsG0180001029.01.T01 AT2G33470 39.744 78 47 0 42 119 25 102 1.88e-14 66.6
MsG0180001029.01.T01 AT2G33470 39.744 78 47 0 42 119 25 102 1.88e-14 66.6

Find 24 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTAGAAATATTGAAAA+TGG 0.207243 1:+14824076 MsG0180001029.01.T01:CDS
TCCAACAACGAATCTAAATT+TGG 0.270826 1:+14824058 MsG0180001029.01.T01:CDS
GAGTGGTAAGAGAGAAATAA+TGG 0.318341 1:+14822862 MsG0180001029.01.T01:CDS
TATTCAGTTCTGCATGTTCT+TGG 0.321499 1:+14823004 MsG0180001029.01.T01:CDS
TGTCATTGAAGAACTTTCTA+TGG 0.343601 1:+14822907 MsG0180001029.01.T01:CDS
ACCAAATTTAGATTCGTTGT+TGG 0.393140 1:-14824059 None:intergenic
TGGGGATGTGAGCAGCTTCA+TGG 0.400980 1:-14822958 None:intergenic
GCTGCAGTAAGGCCTTTGTT+TGG 0.415497 1:+14824132 MsG0180001029.01.T01:CDS
TGTCTCAAAACAGCCATTGT+TGG 0.436087 1:-14823133 None:intergenic
AACGGAAGGCATTGCAAAGA+AGG 0.467244 1:+14824103 MsG0180001029.01.T01:CDS
ATAGAGAGAAAAGGCTTGGT+GGG 0.506837 1:-14822977 None:intergenic
AGGCCTTTGTTTGGCTCACA+AGG 0.523024 1:+14824141 MsG0180001029.01.T01:CDS
GAATAACATATAGAGAGAAA+AGG 0.539202 1:-14822986 None:intergenic
ACATATAGAGAGAAAAGGCT+TGG 0.544110 1:-14822981 None:intergenic
GAAGGGGTCTAGCTGCAGTA+AGG 0.554936 1:+14824121 MsG0180001029.01.T01:CDS
AATATTGAAAATGGAAGCAA+CGG 0.559253 1:+14824085 MsG0180001029.01.T01:CDS
TATAGAGAGAAAAGGCTTGG+TGG 0.564749 1:-14822978 None:intergenic
ACGGAAGGCATTGCAAAGAA+GGG 0.573082 1:+14824104 MsG0180001029.01.T01:CDS
AGATGTGTATCAAAATATCA+AGG 0.576462 1:+14823156 MsG0180001029.01.T01:CDS
TTGAAAATGGAAGCAACGGA+AGG 0.595410 1:+14824089 MsG0180001029.01.T01:CDS
GGTTGGTGAAATATGAAGAG+TGG 0.607296 1:+14822845 None:intergenic
CGGAAGGCATTGCAAAGAAG+GGG 0.639295 1:+14824105 MsG0180001029.01.T01:CDS
TCACCTTGTGAGCCAAACAA+AGG 0.647841 1:-14824144 None:intergenic
TAGAGAGAAAAGGCTTGGTG+GGG 0.682249 1:-14822976 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAGATAAAATGTTA+GGG + Chr1:14823276-14823295 MsG0180001029.01.T01:intron 10.0%
!!! TTTTTTATAGATAAAATGTT+AGG + Chr1:14823275-14823294 MsG0180001029.01.T01:intron 10.0%
!!! AGTTCTGTTATTTAAATTTA+AGG - Chr1:14823789-14823808 None:intergenic 15.0%
!!! TTTTTCCATAATATCAAATT+AGG + Chr1:14823613-14823632 MsG0180001029.01.T01:intron 15.0%
!! AGTATATAATGTAAAGAGAA+GGG - Chr1:14823894-14823913 None:intergenic 20.0%
!! CTCTTTACATTATATACTAT+GGG + Chr1:14823896-14823915 MsG0180001029.01.T01:intron 20.0%
!! TAAAAAAATGCATTAAGAGA+AGG - Chr1:14823262-14823281 None:intergenic 20.0%
!! TAGTATATAATGTAAAGAGA+AGG - Chr1:14823895-14823914 None:intergenic 20.0%
!! TCTCTTTACATTATATACTA+TGG + Chr1:14823895-14823914 MsG0180001029.01.T01:intron 20.0%
!! TCTTTACATTATATACTATG+GGG + Chr1:14823897-14823916 MsG0180001029.01.T01:intron 20.0%
!! TTTAATACACAACTAATCAT+TGG - Chr1:14823996-14824015 None:intergenic 20.0%
!! TTTGGTAGAAATATTGAAAA+TGG + Chr1:14824076-14824095 MsG0180001029.01.T01:CDS 20.0%
! AATATTGAAAATGGAAGCAA+CGG + Chr1:14824085-14824104 MsG0180001029.01.T01:CDS 25.0%
! ACGTGTAAACAGAAAAAATT+GGG + Chr1:14823110-14823129 MsG0180001029.01.T01:intron 25.0%
! AGATGTGTATCAAAATATCA+AGG + Chr1:14823156-14823175 MsG0180001029.01.T01:CDS 25.0%
! ATAGGCCTAATTTGATATTA+TGG - Chr1:14823621-14823640 None:intergenic 25.0%
! ATTAAGAGAAGGTTAAGTAA+GGG - Chr1:14823251-14823270 None:intergenic 25.0%
! GAATAACATATAGAGAGAAA+AGG - Chr1:14822989-14823008 None:intergenic 25.0%
! GTATATAATGTAAAGAGAAG+GGG - Chr1:14823893-14823912 None:intergenic 25.0%
! GTTAGGTGGTTAATAAATTA+AGG + Chr1:14823864-14823883 MsG0180001029.01.T01:intron 25.0%
! TACGTGTAAACAGAAAAAAT+TGG + Chr1:14823109-14823128 MsG0180001029.01.T01:intron 25.0%
! TATGTTAACCCTTAGATTAT+CGG + Chr1:14823398-14823417 MsG0180001029.01.T01:intron 25.0%
! TCTACAAACTTATAGATCAT+TGG - Chr1:14823759-14823778 None:intergenic 25.0%
! TGAATTGAAAGATTTAGCTA+TGG - Chr1:14823194-14823213 None:intergenic 25.0%
!! AACTTTGGTAATTTGAAGTT+AGG + Chr1:14823429-14823448 MsG0180001029.01.T01:intron 25.0%
!! ATATTTTATGAGACCATACT+TGG - Chr1:14823462-14823481 None:intergenic 25.0%
ACCAAATTTAGATTCGTTGT+TGG - Chr1:14824062-14824081 None:intergenic 30.0%
ATGTTAACCCTTAGATTATC+GGG + Chr1:14823399-14823418 MsG0180001029.01.T01:intron 30.0%
CATTAAGAGAAGGTTAAGTA+AGG - Chr1:14823252-14823271 None:intergenic 30.0%
CGAACATAAAAGTTAACCAA+GGG + Chr1:14823835-14823854 MsG0180001029.01.T01:intron 30.0%
GTGAATGTAAATAGAGAGAA+TGG - Chr1:14823737-14823756 None:intergenic 30.0%
TATCTTGCAATCGTAAATCA+AGG - Chr1:14823350-14823369 None:intergenic 30.0%
TCCAACAACGAATCTAAATT+TGG + Chr1:14824058-14824077 MsG0180001029.01.T01:CDS 30.0%
TCGAACATAAAAGTTAACCA+AGG + Chr1:14823834-14823853 MsG0180001029.01.T01:intron 30.0%
TGACATCATAACTACAATTG+AGG + Chr1:14823585-14823604 MsG0180001029.01.T01:intron 30.0%
TGTCATTGAAGAACTTTCTA+TGG + Chr1:14822907-14822926 MsG0180001029.01.T01:CDS 30.0%
TTAACCAAGGGTTATAAGTT+AGG + Chr1:14823847-14823866 MsG0180001029.01.T01:intron 30.0%
TTCCAATCTCTACAATCAAA+CGG - Chr1:14824021-14824040 None:intergenic 30.0%
! GAGATCTAGAGTTTAAATCT+TGG - Chr1:14823319-14823338 None:intergenic 30.0%
! TTTCTAAATTGCTGTTGCAT+AGG - Chr1:14823639-14823658 None:intergenic 30.0%
!!! AGAACTCGAGTTCAATTTTT+TGG - Chr1:14823493-14823512 None:intergenic 30.0%
AAGGTTAAGTAAGGGTTAAC+TGG - Chr1:14823243-14823262 None:intergenic 35.0%
ACATATAGAGAGAAAAGGCT+TGG - Chr1:14822984-14823003 None:intergenic 35.0%
AGAAAAAATTGGGCCAACAA+TGG + Chr1:14823120-14823139 MsG0180001029.01.T01:intron 35.0%
AGGTTAAGTAAGGGTTAACT+GGG - Chr1:14823242-14823261 None:intergenic 35.0%
ATCGTAAATCAAGGAGCTAT+AGG - Chr1:14823341-14823360 None:intergenic 35.0%
CTCTTATCCCGATAATCTAA+GGG - Chr1:14823409-14823428 None:intergenic 35.0%
GAGATCGTCAAAAGATGAAT+CGG - Chr1:14823521-14823540 None:intergenic 35.0%
GAGTGGTAAGAGAGAAATAA+TGG + Chr1:14822862-14822881 MsG0180001029.01.T01:CDS 35.0%
TATTCAGTTCTGCATGTTCT+TGG + Chr1:14823004-14823023 MsG0180001029.01.T01:CDS 35.0%
TCTCTTATCCCGATAATCTA+AGG - Chr1:14823410-14823429 None:intergenic 35.0%
TTATCGGGATAAGAGAACTT+TGG + Chr1:14823414-14823433 MsG0180001029.01.T01:intron 35.0%
TTCCGTTTGATTGTAGAGAT+TGG + Chr1:14824016-14824035 MsG0180001029.01.T01:intron 35.0%
ACCAAGGGTTATAAGTTAGG+TGG + Chr1:14823850-14823869 MsG0180001029.01.T01:intron 40.0%
ACCAAGTGATTGAACTCAAG+TGG - Chr1:14823549-14823568 None:intergenic 40.0%
ACCACCTAACTTATAACCCT+TGG - Chr1:14823854-14823873 None:intergenic 40.0%
ACCACTTGAGTTCAATCACT+TGG + Chr1:14823545-14823564 MsG0180001029.01.T01:intron 40.0%
CGTTCTCATTGAAAGAACAC+AGG - Chr1:14823693-14823712 None:intergenic 40.0%
TATAGAGAGAAAAGGCTTGG+TGG - Chr1:14822981-14823000 None:intergenic 40.0%
TGTCTCAAAACAGCCATTGT+TGG - Chr1:14823136-14823155 None:intergenic 40.0%
TTAAGTAAGGGTTAACTGGG+TGG - Chr1:14823239-14823258 None:intergenic 40.0%
TTGAAAATGGAAGCAACGGA+AGG + Chr1:14824089-14824108 MsG0180001029.01.T01:CDS 40.0%
! ATAGAGAGAAAAGGCTTGGT+GGG - Chr1:14822980-14822999 None:intergenic 40.0%
! GCTTCATGGTTTTCTTCAGT+TGG - Chr1:14822947-14822966 None:intergenic 40.0%
CAGTTCTGCATGTTCTTGGT+AGG + Chr1:14823008-14823027 MsG0180001029.01.T01:intron 45.0%
GTTAGGCTATGAGCCAAGTA+TGG + Chr1:14823446-14823465 MsG0180001029.01.T01:intron 45.0%
TCACCTTGTGAGCCAAACAA+AGG - Chr1:14824147-14824166 None:intergenic 45.0%
!! AACGGAAGGCATTGCAAAGA+AGG + Chr1:14824103-14824122 MsG0180001029.01.T01:CDS 45.0%
!! ACGGAAGGCATTGCAAAGAA+GGG + Chr1:14824104-14824123 MsG0180001029.01.T01:CDS 45.0%
!! TAGAGAGAAAAGGCTTGGTG+GGG - Chr1:14822979-14822998 None:intergenic 45.0%
!! ATTAATCATATTTAAAAAAA+TGG - Chr1:14823963-14823982 None:intergenic 5.0%
!!! TTTTTTTAAATATGATTAAT+TGG + Chr1:14823963-14823982 MsG0180001029.01.T01:intron 5.0%
GCTGCAGTAAGGCCTTTGTT+TGG + Chr1:14824132-14824151 MsG0180001029.01.T01:CDS 50.0%
! AGGCCTTTGTTTGGCTCACA+AGG + Chr1:14824141-14824160 MsG0180001029.01.T01:CDS 50.0%
!! CGGAAGGCATTGCAAAGAAG+GGG + Chr1:14824105-14824124 MsG0180001029.01.T01:CDS 50.0%
GAAGGGGTCTAGCTGCAGTA+AGG + Chr1:14824121-14824140 MsG0180001029.01.T01:CDS 55.0%
TGGGGATGTGAGCAGCTTCA+TGG - Chr1:14822961-14822980 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 14822857 14824166 14822857 ID=MsG0180001029.01;Name=MsG0180001029.01
Chr1 mRNA 14822857 14824166 14822857 ID=MsG0180001029.01.T01;Parent=MsG0180001029.01;Name=MsG0180001029.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|3|0|119
Chr1 exon 14822857 14823025 14822857 ID=MsG0180001029.01.T01:exon:30896;Parent=MsG0180001029.01.T01
Chr1 exon 14823122 14823177 14823122 ID=MsG0180001029.01.T01:exon:30895;Parent=MsG0180001029.01.T01
Chr1 exon 14824032 14824166 14824032 ID=MsG0180001029.01.T01:exon:30894;Parent=MsG0180001029.01.T01
Chr1 CDS 14822857 14823025 14822857 ID=MsG0180001029.01.T01:cds;Parent=MsG0180001029.01.T01
Chr1 CDS 14823122 14823177 14823122 ID=MsG0180001029.01.T01:cds;Parent=MsG0180001029.01.T01
Chr1 CDS 14824032 14824166 14824032 ID=MsG0180001029.01.T01:cds;Parent=MsG0180001029.01.T01
Gene Sequence

>MsG0180001029.01.T01

ATGAAGAGTGGTAAGAGAGAAATAATGGAGAAGAAATCAGAGATAAGTTGTGTCATTGAAGAACTTTCTATGGTTGTAATAGTTAAACCAACTGAAGAAAACCATGAAGCTGCTCACATCCCCACCAAGCCTTTTCTCTCTATATGTTATTCAGTTCTGCATGTTCTTGAAAAAATTGGGCCAACAATGGCTGTTTTGAGACAAGATGTGTATCAAAATATCAAGAGATTGGAACTAATGCATGAATCAAATCCAACAACGAATCTAAATTTGGTAGAAATATTGAAAATGGAAGCAACGGAAGGCATTGCAAAGAAGGGGTCTAGCTGCAGTAAGGCCTTTGTTTGGCTCACAAGGTGA

Protein sequence

>MsG0180001029.01.T01

MKSGKREIMEKKSEISCVIEELSMVVIVKPTEENHEAAHIPTKPFLSICYSVLHVLEKIGPTMAVLRQDVYQNIKRLELMHESNPTTNLNLVEILKMEATEGIAKKGSSCSKAFVWLTR*