Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045090.01.T01 | XP_013445996.1 | 89.865 | 148 | 7 | 3 | 18 | 160 | 18 | 162 | 1.12E-86 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045090.01.T01 | A0A072TT50 | 89.865 | 148 | 7 | 3 | 18 | 160 | 18 | 162 | 5.34e-87 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000984.01 | MsG0880045090.01 | 0.809684 | 1.675230e-50 | 9.900621e-48 |
| MsG0880045090.01 | MsG0880047228.01 | 0.844093 | 9.356105e-59 | 1.494280e-55 |
| MsG0180005678.01 | MsG0880045090.01 | 0.825482 | 4.575206e-54 | 4.175371e-51 |
| MsG0880043920.01 | MsG0880045090.01 | 0.832797 | 7.699016e-56 | 8.697897e-53 |
| MsG0480023575.01 | MsG0880045090.01 | 0.804582 | 2.016095e-49 | 1.043367e-46 |
| MsG0580028884.01 | MsG0880045090.01 | 0.821192 | 4.600179e-53 | 3.717272e-50 |
| MsG0580028916.01 | MsG0880045090.01 | 0.806360 | 8.544105e-50 | 4.628139e-47 |
| MsG0580029739.01 | MsG0880045090.01 | 0.815892 | 7.324174e-52 | 5.112525e-49 |
| MsG0280006924.01 | MsG0880045090.01 | 0.862470 | 4.955850e-64 | 1.453073e-60 |
| MsG0280008751.01 | MsG0880045090.01 | 0.812046 | 5.163040e-51 | 3.247885e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045090.01.T01 | MTR_8g064760 | 89.865 | 148 | 7 | 3 | 18 | 160 | 18 | 162 | 1.35e-90 | 261 |
| MsG0880045090.01.T01 | MTR_5g034200 | 37.500 | 160 | 70 | 6 | 18 | 159 | 20 | 167 | 1.80e-19 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045090.01.T01 | AT1G23530 | 38.636 | 132 | 61 | 7 | 1 | 121 | 1 | 123 | 1.86e-15 | 70.5 |
Find 38 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATTCAAATGGATCTTT+TGG | 0.191350 | 8:-54055822 | MsG0880045090.01.T01:CDS |
| GGCTGTTGTGTTGATGATTT+TGG | 0.220534 | 8:+54055694 | None:intergenic |
| CCTCTAGGCCTAGTAATTAT+TGG | 0.294250 | 8:+54055567 | None:intergenic |
| CTGCCTACTCGACGGCTTAA+CGG | 0.302986 | 8:+54055659 | None:intergenic |
| TGCTATAATACTAGTGATTT+CGG | 0.341197 | 8:-54055781 | MsG0880045090.01.T01:CDS |
| GTTGATTTGTGTTGGTTGTT+GGG | 0.344438 | 8:+54055853 | None:intergenic |
| TGCCTACTCGACGGCTTAAC+GGG | 0.376180 | 8:+54055660 | None:intergenic |
| GGTTGATTTGTGTTGGTTGT+TGG | 0.385321 | 8:+54055852 | None:intergenic |
| CAAAAGATCCATTTGAATGA+TGG | 0.405902 | 8:+54055823 | None:intergenic |
| GCTTAACGGGCCGTCTCTGC+AGG | 0.432684 | 8:+54055673 | None:intergenic |
| CCATCTTCCCATCATTCAAA+TGG | 0.441379 | 8:-54055831 | MsG0880045090.01.T01:CDS |
| AATCCAAATATGTCTATTCA+AGG | 0.460611 | 8:-54055915 | None:intergenic |
| AAAAGATCCATTTGAATGAT+GGG | 0.472353 | 8:+54055824 | None:intergenic |
| CAACCACATGGTCATGGTAA+TGG | 0.479592 | 8:-54055510 | MsG0880045090.01.T01:CDS |
| TATGGTAGCGTCGGTTGCAA+AGG | 0.481102 | 8:+54055736 | None:intergenic |
| GTTGTTGGGTAAACTTGAAC+AGG | 0.481158 | 8:+54055867 | None:intergenic |
| GGAAGATGGTTGATTTGTGT+TGG | 0.486873 | 8:+54055845 | None:intergenic |
| AAATGAAATCTGATCATGAA+AGG | 0.501074 | 8:-54055470 | MsG0880045090.01.T01:CDS |
| CCATTTGAATGATGGGAAGA+TGG | 0.517579 | 8:+54055831 | None:intergenic |
| AATGAAATCTGATCATGAAA+GGG | 0.536387 | 8:-54055469 | MsG0880045090.01.T01:CDS |
| GGTTCAAAAGCAAGTCCTCT+AGG | 0.539679 | 8:+54055552 | None:intergenic |
| TGCCTCCACCAATAATTACT+AGG | 0.540441 | 8:-54055575 | MsG0880045090.01.T01:CDS |
| CTAGGCCTAGTAATTATTGG+TGG | 0.551916 | 8:+54055570 | None:intergenic |
| AGAGAAGATGACATTGAACT+TGG | 0.557715 | 8:-54055612 | MsG0880045090.01.T01:CDS |
| ATCTGTTGCTGCCTACTCGA+CGG | 0.571035 | 8:+54055651 | None:intergenic |
| GGATCTCAACCACATGGTCA+TGG | 0.573596 | 8:-54055516 | MsG0880045090.01.T01:CDS |
| GAAAGGGATCAGCATAGAGC+TGG | 0.575810 | 8:-54055453 | MsG0880045090.01.T01:CDS |
| GGCCTAGTAATTATTGGTGG+AGG | 0.576499 | 8:+54055573 | None:intergenic |
| AACACAACAGCCTGCAGAGA+CGG | 0.587321 | 8:-54055683 | MsG0880045090.01.T01:CDS |
| TTTGAACCGCCACGCACTGG+AGG | 0.618187 | 8:-54055537 | MsG0880045090.01.T01:CDS |
| TAAACTTGAACAGGTTGTTG+TGG | 0.654880 | 8:+54055876 | None:intergenic |
| CCAATAATTACTAGGCCTAG+AGG | 0.665419 | 8:-54055567 | MsG0880045090.01.T01:CDS |
| GGTTGAGATCCTCCAGTGCG+TGG | 0.667179 | 8:+54055528 | None:intergenic |
| GGCCCGTTAAGCCGTCGAGT+AGG | 0.668761 | 8:-54055662 | MsG0880045090.01.T01:CDS |
| AAATCACTAGTATTATAGCA+AGG | 0.678683 | 8:+54055784 | None:intergenic |
| TGAGATCCTCCAGTGCGTGG+CGG | 0.689150 | 8:+54055531 | None:intergenic |
| ACTGGAGGATCTCAACCACA+TGG | 0.723136 | 8:-54055522 | MsG0880045090.01.T01:CDS |
| TTACCATTACCATGACCATG+TGG | 0.751168 | 8:+54055507 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAGATCCATTTGAATGAT+GGG | + | Chr8:54055531-54055550 | None:intergenic | 25.0% |
| ! | AAATCACTAGTATTATAGCA+AGG | + | Chr8:54055571-54055590 | None:intergenic | 25.0% |
| ! | AAATGAAATCTGATCATGAA+AGG | - | Chr8:54055882-54055901 | MsG0880045090.01.T01:CDS | 25.0% |
| ! | AATGAAATCTGATCATGAAA+GGG | - | Chr8:54055883-54055902 | MsG0880045090.01.T01:CDS | 25.0% |
| ! | TGCTATAATACTAGTGATTT+CGG | - | Chr8:54055571-54055590 | MsG0880045090.01.T01:CDS | 25.0% |
| !! | ATCATTCAAATGGATCTTTT+GGG | - | Chr8:54055531-54055550 | MsG0880045090.01.T01:CDS | 25.0% |
| CAAAAGATCCATTTGAATGA+TGG | + | Chr8:54055532-54055551 | None:intergenic | 30.0% | |
| ! | CATCATTCAAATGGATCTTT+TGG | - | Chr8:54055530-54055549 | MsG0880045090.01.T01:CDS | 30.0% |
| !!! | TGTCGTTGTTGTTGTTTTTA+TGG | + | Chr8:54055637-54055656 | None:intergenic | 30.0% |
| AGAGAAGATGACATTGAACT+TGG | - | Chr8:54055740-54055759 | MsG0880045090.01.T01:CDS | 35.0% | |
| TAAACTTGAACAGGTTGTTG+TGG | + | Chr8:54055479-54055498 | None:intergenic | 35.0% | |
| !! | GTTGATTTGTGTTGGTTGTT+GGG | + | Chr8:54055502-54055521 | None:intergenic | 35.0% |
| !!! | TGTTGTTTTTATGGTAGCGT+CGG | + | Chr8:54055628-54055647 | None:intergenic | 35.0% |
| CCAATAATTACTAGGCCTAG+AGG | - | Chr8:54055785-54055804 | MsG0880045090.01.T01:CDS | 40.0% | |
| CCATCTTCCCATCATTCAAA+TGG | - | Chr8:54055521-54055540 | MsG0880045090.01.T01:CDS | 40.0% | |
| CCTCTAGGCCTAGTAATTAT+TGG | + | Chr8:54055788-54055807 | None:intergenic | 40.0% | |
| CTAGGCCTAGTAATTATTGG+TGG | + | Chr8:54055785-54055804 | None:intergenic | 40.0% | |
| GTTGTTGGGTAAACTTGAAC+AGG | + | Chr8:54055488-54055507 | None:intergenic | 40.0% | |
| TGCCTCCACCAATAATTACT+AGG | - | Chr8:54055777-54055796 | MsG0880045090.01.T01:CDS | 40.0% | |
| TTACCATTACCATGACCATG+TGG | + | Chr8:54055848-54055867 | None:intergenic | 40.0% | |
| ! | CCATTTGAATGATGGGAAGA+TGG | + | Chr8:54055524-54055543 | None:intergenic | 40.0% |
| !! | GGAAGATGGTTGATTTGTGT+TGG | + | Chr8:54055510-54055529 | None:intergenic | 40.0% |
| !! | GGTTGATTTGTGTTGGTTGT+TGG | + | Chr8:54055503-54055522 | None:intergenic | 40.0% |
| !! | TCGGTAGTTGCTTGTTTTCT+CGG | - | Chr8:54055590-54055609 | MsG0880045090.01.T01:CDS | 40.0% |
| !!! | GGCTGTTGTGTTGATGATTT+TGG | + | Chr8:54055661-54055680 | None:intergenic | 40.0% |
| CAACCACATGGTCATGGTAA+TGG | - | Chr8:54055842-54055861 | MsG0880045090.01.T01:CDS | 45.0% | |
| GGCCTAGTAATTATTGGTGG+AGG | + | Chr8:54055782-54055801 | None:intergenic | 45.0% | |
| GGTTCAAAAGCAAGTCCTCT+AGG | + | Chr8:54055803-54055822 | None:intergenic | 45.0% | |
| AACACAACAGCCTGCAGAGA+CGG | - | Chr8:54055669-54055688 | MsG0880045090.01.T01:CDS | 50.0% | |
| ACTGGAGGATCTCAACCACA+TGG | - | Chr8:54055830-54055849 | MsG0880045090.01.T01:CDS | 50.0% | |
| ATCTGTTGCTGCCTACTCGA+CGG | + | Chr8:54055704-54055723 | None:intergenic | 50.0% | |
| GAAAGGGATCAGCATAGAGC+TGG | - | Chr8:54055899-54055918 | MsG0880045090.01.T01:CDS | 50.0% | |
| GGATCTCAACCACATGGTCA+TGG | - | Chr8:54055836-54055855 | MsG0880045090.01.T01:CDS | 50.0% | |
| !! | TATGGTAGCGTCGGTTGCAA+AGG | + | Chr8:54055619-54055638 | None:intergenic | 50.0% |
| CTGCCTACTCGACGGCTTAA+CGG | + | Chr8:54055696-54055715 | None:intergenic | 55.0% | |
| TGCCTACTCGACGGCTTAAC+GGG | + | Chr8:54055695-54055714 | None:intergenic | 55.0% | |
| GGTTGAGATCCTCCAGTGCG+TGG | + | Chr8:54055827-54055846 | None:intergenic | 60.0% | |
| TGAGATCCTCCAGTGCGTGG+CGG | + | Chr8:54055824-54055843 | None:intergenic | 60.0% | |
| TTTGAACCGCCACGCACTGG+AGG | - | Chr8:54055815-54055834 | MsG0880045090.01.T01:CDS | 60.0% | |
| ! | GCTTTTGAACCGCCACGCAC+TGG | - | Chr8:54055812-54055831 | MsG0880045090.01.T01:CDS | 60.0% |
| GGCCCGTTAAGCCGTCGAGT+AGG | - | Chr8:54055690-54055709 | MsG0880045090.01.T01:CDS | 65.0% | |
| ! | GCTTAACGGGCCGTCTCTGC+AGG | + | Chr8:54055682-54055701 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 54055446 | 54055928 | 54055446 | ID=MsG0880045090.01;Name=MsG0880045090.01 |
| Chr8 | mRNA | 54055446 | 54055928 | 54055446 | ID=MsG0880045090.01.T01;Parent=MsG0880045090.01;Name=MsG0880045090.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|160 |
| Chr8 | exon | 54055446 | 54055928 | 54055446 | ID=MsG0880045090.01.T01:exon:23290;Parent=MsG0880045090.01.T01 |
| Chr8 | CDS | 54055446 | 54055928 | 54055446 | ID=MsG0880045090.01.T01:cds;Parent=MsG0880045090.01.T01 |
| Gene Sequence |
| Protein sequence |
