AlfalfaGEDB | MsG0880047228.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047228.01

KEGG: -
GO: GO:0005515
PFAM: PF00917

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047228.01.T01	XP_003609855.1	96.785	311	10	0	1	311	1	311	0	632

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047228.01.T01	Q9FPT1	31.875	160	93	5	1	154	32	181	3.13E-15	79.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047228.01.T01	G7JQ23	96.785	311	10	0	1	311	1	311	0.0	632

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000984.01	MsG0880047228.01	0.810392	1.179358e-50	7.100038e-48
MsG0880045090.01	MsG0880047228.01	0.844093	9.356105e-59	1.494280e-55
MsG0880046940.01	MsG0880047228.01	0.867809	1.040452e-65	3.680128e-62
MsG0880047080.01	MsG0880047228.01	0.826807	2.214632e-54	2.098935e-51
MsG0180003863.01	MsG0880047228.01	0.800567	1.356468e-48	6.336741e-46
MsG0180004098.01	MsG0880047228.01	0.814397	1.572743e-51	1.054188e-48
MsG0180004102.01	MsG0880047228.01	0.814246	1.699008e-51	1.134118e-48
MsG0180005633.01	MsG0880047228.01	0.823309	1.484311e-53	1.273345e-50
MsG0180005678.01	MsG0880047228.01	0.808893	2.475773e-50	1.432503e-47
MsG0180005924.01	MsG0880047228.01	0.883574	3.997249e-71	2.548524e-67
MsG0880042098.01	MsG0880047228.01	0.814169	1.766623e-51	1.176757e-48
MsG0880043920.01	MsG0880047228.01	0.847776	9.335355e-60	1.677135e-56
MsG0380015736.01	MsG0880047228.01	0.807506	4.890235e-50	2.728681e-47
MsG0380016480.01	MsG0880047228.01	0.811987	5.317991e-51	3.340182e-48
MsG0380017421.01	MsG0880047228.01	0.870353	1.555741e-66	6.032635e-63
MsG0580026310.01	MsG0880047228.01	0.834071	3.705459e-56	4.348980e-53
MsG0580027461.01	MsG0880047228.01	0.814806	1.276969e-51	8.653999e-49
MsG0580028884.01	MsG0880047228.01	0.886522	3.178195e-72	2.278131e-68
MsG0580029494.01	MsG0880047228.01	0.841689	4.080397e-58	6.042117e-55
MsG0580029739.01	MsG0880047228.01	0.856052	4.173998e-62	9.835084e-59
MsG0280006924.01	MsG0880047228.01	0.875034	4.233874e-68	1.948473e-64
MsG0280008740.01	MsG0880047228.01	0.835518	1.601659e-56	1.963261e-53
MsG0280008751.01	MsG0880047228.01	0.826265	2.982751e-54	2.783494e-51
MsG0780037371.01	MsG0880047228.01	0.876700	1.133920e-68	5.550580e-65

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047228.01.T01	MTR_4g123750	96.785	311	10	0	1	311	1	311	0.0	632
MsG0880047228.01.T01	MTR_8g006190	39.655	290	156	4	35	307	5	292	6.03e-65	211
MsG0880047228.01.T01	MTR_8g006190	23.051	295	192	9	28	307	165	439	1.83e-11	64.7
MsG0880047228.01.T01	MTR_8g006190	38.321	274	150	4	51	307	13	284	3.03e-55	185
MsG0880047228.01.T01	MTR_8g006190	23.051	295	192	9	28	307	157	431	1.67e-11	65.1
MsG0880047228.01.T01	MTR_7g096550	94.000	50	3	0	1	50	1	50	3.82e-27	100
MsG0880047228.01.T01	MTR_5g091450	35.036	137	80	4	16	152	30	157	7.60e-19	87.4
MsG0880047228.01.T01	MTR_5g091450	30.000	130	88	2	174	302	31	158	2.00e-12	68.2
MsG0880047228.01.T01	MTR_5g086700	32.414	145	88	4	12	156	54	188	6.94e-17	81.6
MsG0880047228.01.T01	MTR_5g086700	31.915	141	92	2	169	308	54	191	1.43e-16	80.9
MsG0880047228.01.T01	MTR_5g086700	32.414	145	88	4	12	156	54	188	7.40e-17	81.6
MsG0880047228.01.T01	MTR_5g086700	31.915	141	92	2	169	308	54	191	1.48e-16	80.9
MsG0880047228.01.T01	MTR_3g079840	31.737	167	94	6	9	165	40	196	1.47e-16	80.9
MsG0880047228.01.T01	MTR_3g079840	32.624	141	91	2	169	308	49	186	3.28e-16	79.7
MsG0880047228.01.T01	MTR_3g079840	31.737	167	94	6	9	165	41	197	1.48e-16	80.9
MsG0880047228.01.T01	MTR_3g079840	32.624	141	91	2	169	308	50	187	3.37e-16	79.7
MsG0880047228.01.T01	MTR_5g091180	32.624	141	82	5	14	152	41	170	4.34e-16	79.3
MsG0880047228.01.T01	MTR_5g091180	30.370	135	89	4	170	302	40	171	3.95e-12	67.4
MsG0880047228.01.T01	MTR_4g123790	73.810	42	11	0	164	205	12	53	1.83e-15	71.2
MsG0880047228.01.T01	MTR_1g042160	24.516	155	109	3	20	174	14	160	2.90e-15	75.5
MsG0880047228.01.T01	MTR_1g041240	28.767	146	92	4	13	157	52	186	4.66e-15	76.3
MsG0880047228.01.T01	MTR_1g041240	25.405	185	129	4	129	308	8	188	1.19e-12	68.9
MsG0880047228.01.T01	MTR_1g041240	28.767	146	92	4	13	157	51	185	4.84e-15	76.3
MsG0880047228.01.T01	MTR_1g041240	25.946	185	127	5	129	308	8	187	8.90e-13	69.3
MsG0880047228.01.T01	MTR_1g041240	28.767	146	92	4	13	157	50	184	5.02e-15	76.3
MsG0880047228.01.T01	MTR_1g041240	28.671	143	97	3	167	308	48	186	1.32e-12	68.9
MsG0880047228.01.T01	MTR_5g091550	34.211	152	90	5	40	190	37	179	9.40e-14	72.0
MsG0880047228.01.T01	MTR_5g091550	29.921	127	85	2	177	302	20	143	1.98e-11	64.7
MsG0880047228.01.T01	MTR_5g091550	32.450	151	89	5	40	190	37	174	1.31e-13	71.6
MsG0880047228.01.T01	MTR_5g091550	29.921	127	85	2	177	302	20	143	2.21e-11	64.7
MsG0880047228.01.T01	MTR_3g106700	19.930	286	185	8	39	301	432	696	8.11e-12	66.6
MsG0880047228.01.T01	MTR_7g107200	27.143	140	95	3	23	162	17	149	4.94e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047228.01.T01	AT3G17380	52.459	305	142	1	6	310	8	309	7.71e-112	327
MsG0880047228.01.T01	AT5G26280	32.353	306	201	4	7	311	49	349	2.37e-49	168
MsG0880047228.01.T01	AT5G26260	33.224	304	197	4	9	311	52	350	4.85e-49	167
MsG0880047228.01.T01	AT5G26280	32.353	306	201	4	7	311	68	368	6.21e-49	167
MsG0880047228.01.T01	AT5G26290	32.026	306	197	4	6	311	41	335	9.37e-45	155
MsG0880047228.01.T01	AT4G09780	34.641	306	183	10	8	309	53	345	5.91e-43	152
MsG0880047228.01.T01	AT2G04170	31.864	295	195	4	17	310	7	296	8.42e-43	149
MsG0880047228.01.T01	AT2G04170	31.864	295	195	4	17	310	7	296	8.42e-43	149
MsG0880047228.01.T01	AT5G26300	30.392	306	206	5	7	311	49	348	1.20e-42	150
MsG0880047228.01.T01	AT4G09770	33.333	306	186	10	8	309	26	317	4.16e-42	148
MsG0880047228.01.T01	AT4G09770	33.333	303	184	10	11	309	3	291	4.88e-42	147
MsG0880047228.01.T01	AT4G09770	33.333	303	184	10	11	309	3	291	4.88e-42	147
MsG0880047228.01.T01	AT2G04170	31.864	295	195	4	17	310	89	378	5.77e-42	149
MsG0880047228.01.T01	AT2G04170	31.864	295	195	4	17	310	129	418	1.28e-41	149
MsG0880047228.01.T01	AT2G04170	31.864	295	195	4	17	310	129	418	1.28e-41	149
MsG0880047228.01.T01	AT4G09780	33.333	315	186	9	8	309	53	356	7.54e-41	147
MsG0880047228.01.T01	AT3G20370	30.897	301	195	7	10	304	56	349	1.50e-40	145
MsG0880047228.01.T01	AT3G28220	32.404	287	183	7	17	300	80	358	2.00e-40	145
MsG0880047228.01.T01	AT3G20370	30.897	301	195	7	10	304	78	371	2.87e-40	145
MsG0880047228.01.T01	AT3G20360	30.341	323	206	9	1	310	45	361	1.32e-39	142
MsG0880047228.01.T01	AT4G09770	35.060	251	148	8	8	255	26	264	1.94e-38	137
MsG0880047228.01.T01	AT4G09770	35.081	248	146	8	11	255	3	238	2.68e-38	136
MsG0880047228.01.T01	AT3G22080	28.808	302	201	7	10	305	12	305	5.30e-38	137
MsG0880047228.01.T01	AT2G04170	33.884	242	155	3	14	255	126	362	6.09e-37	135
MsG0880047228.01.T01	AT5G26320	28.247	308	213	5	7	311	49	351	7.66e-37	135
MsG0880047228.01.T01	AT5G26280	28.431	306	190	5	7	311	49	326	1.46e-35	131
MsG0880047228.01.T01	AT2G04190	29.642	307	205	7	8	310	110	409	1.77e-35	132
MsG0880047228.01.T01	AT2G15710	30.420	286	179	7	5	287	86	354	1.38e-33	127
MsG0880047228.01.T01	AT4G00780	30.137	292	183	8	20	307	22	296	3.15e-32	121
MsG0880047228.01.T01	AT1G69650	27.273	308	184	9	8	307	13	288	8.63e-32	120
MsG0880047228.01.T01	AT4G01390	29.236	301	173	8	20	306	22	296	3.63e-31	119
MsG0880047228.01.T01	AT2G04170	37.306	193	116	3	14	206	126	313	1.38e-30	117
MsG0880047228.01.T01	AT3G22085	28.996	269	164	7	6	272	7	250	1.84e-29	114
MsG0880047228.01.T01	AT1G58270	28.383	303	196	9	17	311	94	383	2.86e-29	115
MsG0880047228.01.T01	AT3G20380	24.830	294	215	3	8	301	77	364	1.25e-28	113
MsG0880047228.01.T01	AT1G65150	31.250	240	148	7	36	272	32	257	3.01e-28	111
MsG0880047228.01.T01	AT1G65150	31.250	240	148	7	36	272	32	257	3.01e-28	111
MsG0880047228.01.T01	AT2G32870	25.312	320	212	7	5	310	110	416	2.16e-26	108
MsG0880047228.01.T01	AT1G65370	29.258	229	146	6	78	304	7	221	2.34e-25	101
MsG0880047228.01.T01	AT2G32880	25.466	322	211	10	5	310	10	318	3.01e-24	100
MsG0880047228.01.T01	AT1G69660	26.872	227	159	4	82	307	5	225	3.63e-23	95.9
MsG0880047228.01.T01	AT2G32880	26.718	262	168	8	5	255	10	258	2.17e-21	92.4
MsG0880047228.01.T01	AT2G32870	26.538	260	169	6	5	255	110	356	1.89e-20	90.9
MsG0880047228.01.T01	AT3G46190	25.510	294	178	7	14	304	29	284	1.76e-19	87.0
MsG0880047228.01.T01	AT3G46190	25.510	294	178	7	14	304	49	304	2.23e-19	87.0
MsG0880047228.01.T01	AT1G65050	33.636	110	69	2	36	145	32	137	2.16e-17	78.6
MsG0880047228.01.T01	AT1G65050	33.636	110	69	2	36	145	32	137	2.16e-17	78.6
MsG0880047228.01.T01	AT5G06600	31.875	160	93	5	1	154	32	181	2.92e-16	80.1
MsG0880047228.01.T01	AT5G06600	32.143	140	91	2	170	308	50	186	7.49e-15	75.9
MsG0880047228.01.T01	AT5G06600	31.875	160	93	5	1	154	31	180	3.04e-16	79.7
MsG0880047228.01.T01	AT5G06600	32.143	140	91	2	170	308	49	185	6.03e-15	75.9
MsG0880047228.01.T01	AT5G06600	31.875	160	93	5	1	154	32	181	3.19e-16	79.7
MsG0880047228.01.T01	AT5G06600	32.143	140	91	2	170	308	50	186	6.32e-15	75.9
MsG0880047228.01.T01	AT3G11910	31.783	129	81	3	26	154	58	179	4.87e-16	79.3
MsG0880047228.01.T01	AT3G11910	31.429	140	92	2	170	308	48	184	1.67e-14	74.7
MsG0880047228.01.T01	AT3G11910	31.783	129	81	3	26	154	58	179	4.87e-16	79.3
MsG0880047228.01.T01	AT3G11910	31.429	140	92	2	170	308	48	184	1.67e-14	74.7
MsG0880047228.01.T01	AT3G11910	31.783	129	81	3	26	154	59	180	4.92e-16	79.3
MsG0880047228.01.T01	AT3G11910	31.429	140	92	2	170	308	49	185	1.69e-14	74.7
MsG0880047228.01.T01	AT3G11910	31.783	129	81	3	26	154	59	180	4.92e-16	79.3
MsG0880047228.01.T01	AT3G11910	31.429	140	92	2	170	308	49	185	1.69e-14	74.7
MsG0880047228.01.T01	AT3G58340	33.103	145	89	3	169	310	1	140	1.14e-15	76.6
MsG0880047228.01.T01	AT3G58340	33.103	145	89	3	169	310	1	140	1.49e-15	76.3
MsG0880047228.01.T01	AT3G58210	32.168	143	95	2	169	310	1	142	1.24e-14	73.9
MsG0880047228.01.T01	AT3G58210	33.333	132	78	4	24	154	13	135	2.98e-12	66.6
MsG0880047228.01.T01	AT1G31390	32.540	126	80	2	169	291	1	124	7.43e-13	67.8
MsG0880047228.01.T01	AT3G58370	32.192	146	94	2	168	310	1	144	7.52e-13	68.2
MsG0880047228.01.T01	AT3G58370	28.431	204	133	5	24	227	14	204	3.09e-11	63.5
MsG0880047228.01.T01	AT3G58410	31.618	136	88	2	176	311	8	138	1.44e-12	67.8
MsG0880047228.01.T01	AT3G58360	31.724	145	91	3	169	310	1	140	1.88e-12	67.0
MsG0880047228.01.T01	AT2G05420	32.374	139	87	3	178	310	11	148	1.18e-11	64.7
MsG0880047228.01.T01	AT1G31400	31.690	142	89	3	169	307	1	137	3.32e-11	63.2
MsG0880047228.01.T01	AT3G58250	30.935	139	90	3	176	311	10	145	9.44e-11	62.4

Find 74 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGTCTGTGCATATATTT+TGG	0.140548	8:-83595490	None:intergenic
GTCTACTTGCAGCTAATTAT+TGG	0.237931	8:+83595721	MsG0880047228.01.T01:intron
GATGTTGAGGTTACAGTTCT+TGG	0.266368	8:+83595846	MsG0880047228.01.T01:CDS
TTTAATAGATGATGTGAAAA+AGG	0.278698	8:+83594969	MsG0880047228.01.T01:intron
TCCTCTCAAGGATTTCAATC+TGG	0.293183	8:+83595041	MsG0880047228.01.T01:CDS
AGCAATCCAAGCATCAGTTT+TGG	0.342021	8:+83595532	MsG0880047228.01.T01:CDS
AATCTCCCATACATGCTTGT+AGG	0.374747	8:-83595178	None:intergenic
CCCTCCCCTACAAGCATGTA+TGG	0.374887	8:+83595172	MsG0880047228.01.T01:CDS
GATTAAAGACTTCTCAAAGT+TGG	0.382721	8:+83595197	MsG0880047228.01.T01:CDS
GAGGAACAAACCGTCATCTT+TGG	0.387041	8:-83594044	None:intergenic
CTGTTGGGTTAGCTAAAGTT+AGG	0.387750	8:-83595452	None:intergenic
ATTACTGCACAAAATAAATC+TGG	0.389938	8:-83595775	None:intergenic
TGTATATTGTATACCATTTG+TGG	0.403396	8:-83594558	None:intergenic
AGACTTCTCAAAGTTGGATT+CGG	0.407331	8:+83595203	MsG0880047228.01.T01:CDS
GGATCATATTTCACTATACT+TGG	0.407706	8:+83594721	MsG0880047228.01.T01:CDS
TTGTAGTTTCCAACATTGAA+TGG	0.413486	8:-83595243	None:intergenic
TTCACAGAATGAAGGTTGAA+TGG	0.418718	8:+83595001	MsG0880047228.01.T01:CDS
TTCTATAGAGAGATATGAAT+CGG	0.423671	8:+83594516	MsG0880047228.01.T01:CDS
GTGGATGATATATGTGCATT+TGG	0.423766	8:+83595081	MsG0880047228.01.T01:CDS
TCACAGAATGAAGGTTGAAT+GGG	0.425550	8:+83595002	MsG0880047228.01.T01:CDS
CCTCTCAAGGATTTCAATCT+GGG	0.426272	8:+83595042	MsG0880047228.01.T01:CDS
GAGCAGAAGTCTTTGTTTGT+AGG	0.431985	8:+83595103	MsG0880047228.01.T01:CDS
GTATATGTAATTATTATGTA+TGG	0.432832	8:-83594006	None:intergenic
ACCTTTCCAAAACTGATGCT+TGG	0.443667	8:-83595538	None:intergenic
ATCTCCCATACATGCTTGTA+GGG	0.444436	8:-83595177	None:intergenic
AGCAGAAGTCTTTGTTTGTA+GGG	0.457138	8:+83595104	MsG0880047228.01.T01:CDS
GTAAAGTACCTTGGAGAACT+AGG	0.458919	8:-83594832	None:intergenic
GATTTCAATCTGGGCTCTAA+AGG	0.459503	8:+83595051	MsG0880047228.01.T01:CDS
AAGCTCACTTCATCCTGGTT+GGG	0.466058	8:+83594757	MsG0880047228.01.T01:CDS
TAATTTCACCAGTCAATACC+TGG	0.466177	8:+83595794	MsG0880047228.01.T01:CDS
TATTTCACTATACTTGGCAT+TGG	0.479251	8:+83594727	MsG0880047228.01.T01:CDS
TGATCAATTTATTCCTCTCA+AGG	0.485622	8:+83595029	MsG0880047228.01.T01:CDS
ATACAAGAATGTAGTTGCAT+TGG	0.491129	8:+83595910	MsG0880047228.01.T01:three_prime_UTR
CAAGCTCACTTCATCCTGGT+TGG	0.493535	8:+83594756	MsG0880047228.01.T01:CDS
TCTATAGAGAGATATGAATC+GGG	0.505763	8:+83594517	MsG0880047228.01.T01:CDS
AAACTGTATCCCAAAGGAAA+AGG	0.507112	8:+83595399	MsG0880047228.01.T01:CDS
GGAAATGAGTTCACAAATGT+AGG	0.512337	8:-83593985	None:intergenic
ACCGTCATCTTTGGTATCCA+TGG	0.513134	8:-83594035	None:intergenic
TTGAAGCTGGAGGCCACAAA+TGG	0.515480	8:+83594545	MsG0880047228.01.T01:CDS
AGAATCTTTGATCATGATGA+AGG	0.521528	8:+83595146	MsG0880047228.01.T01:CDS
GACTCTAAGCCATTCAATGT+TGG	0.526449	8:+83595234	MsG0880047228.01.T01:CDS
CATTTGCTTTGTAGATGTTG+AGG	0.531213	8:+83595833	MsG0880047228.01.T01:CDS
AGGGAAAACTACACAGGTAA+AGG	0.536644	8:+83595123	MsG0880047228.01.T01:CDS
CAAAGGAAAAGGTACTGACT+CGG	0.538845	8:+83595410	MsG0880047228.01.T01:CDS
CCCATACATGCTTGTAGGGG+AGG	0.542077	8:-83595173	None:intergenic
GAATCGGGGAAGTTTGAAGC+TGG	0.551406	8:+83594532	MsG0880047228.01.T01:CDS
TAGATACCCCAGGTATTGAC+TGG	0.568960	8:-83595802	None:intergenic
AAACAAAAGCAAGAATGTAA+AGG	0.570463	8:+83594700	MsG0880047228.01.T01:CDS
GAATCAAGCTCACTTCATCC+TGG	0.576113	8:+83594752	MsG0880047228.01.T01:CDS
CACAGAATGAAGGTTGAATG+GGG	0.578801	8:+83595003	MsG0880047228.01.T01:CDS
CCATACATGCTTGTAGGGGA+GGG	0.581374	8:-83595172	None:intergenic
AAAGGAAAAGGTACTGACTC+GGG	0.585829	8:+83595411	MsG0880047228.01.T01:CDS
GTTTGTAGGGAAAACTACAC+AGG	0.592836	8:+83595117	MsG0880047228.01.T01:CDS
TTTACATAGATTTCCCAACC+AGG	0.601857	8:-83594770	None:intergenic
GGGCTCTAAAGGTTATCTAG+TGG	0.608318	8:+83595062	MsG0880047228.01.T01:CDS
ATTACATATACATCTAGCCA+TGG	0.612494	8:+83594018	MsG0880047228.01.T01:exon
AATTTCACCAGTCAATACCT+GGG	0.612530	8:+83595795	MsG0880047228.01.T01:CDS
CCTCCCCTACAAGCATGTAT+GGG	0.619373	8:+83595173	MsG0880047228.01.T01:CDS
TGAGGTTACAGTTCTTGGAG+TGG	0.629632	8:+83595851	MsG0880047228.01.T01:CDS
ATACCTGGGGTATCTAGTGA+AGG	0.632705	8:+83595809	MsG0880047228.01.T01:CDS
CAGATAAAACTGTATCCCAA+AGG	0.633902	8:+83595393	MsG0880047228.01.T01:CDS
TGTCCTTCACTAGATACCCC+AGG	0.633913	8:-83595812	None:intergenic
TCGGGGAAGTTTGAAGCTGG+AGG	0.635431	8:+83594535	MsG0880047228.01.T01:CDS
GACAACTACCTAGTTCTCCA+AGG	0.638057	8:+83594824	MsG0880047228.01.T01:CDS
GCCATGGATACCAAAGATGA+CGG	0.641327	8:+83594034	MsG0880047228.01.T01:exon
TCTCTATAGAATTAGTAGTG+AGG	0.653359	8:-83594504	None:intergenic
CCCAGATTGAAATCCTTGAG+AGG	0.660903	8:-83595042	None:intergenic
TGTGCCATGTTCATGACTTG+AGG	0.660953	8:-83595751	None:intergenic
AAGTTAGTTATGTATCCAAG+TGG	0.687329	8:+83594677	MsG0880047228.01.T01:CDS
TCAATGTTGGAAACTACAAG+TGG	0.691657	8:+83595247	MsG0880047228.01.T01:CDS
CTATAGAGAGATATGAATCG+GGG	0.694653	8:+83594518	MsG0880047228.01.T01:CDS
TCTCCCATACATGCTTGTAG+GGG	0.699554	8:-83595176	None:intergenic
AGTGCCTCAAGTCATGAACA+TGG	0.700230	8:+83595747	MsG0880047228.01.T01:CDS
ATTTCACCAGTCAATACCTG+GGG	0.717112	8:+83595796	MsG0880047228.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAAAAAAAATTAACTCA+AGG	+	Chr8:83594327-83594346	MsG0880047228.01.T01:intron	10.0%
!!!	TTCATGAAATTATTTTTATA+AGG	-	Chr8:83594599-83594618	None:intergenic	10.0%
!!	CTTATAAAAAATAAAATTGC+AGG	+	Chr8:83594415-83594434	MsG0880047228.01.T01:intron	15.0%
!!	GTATATGTAATTATTATGTA+TGG	-	Chr8:83594009-83594028	None:intergenic	15.0%
!!!	AATTTCATGAATGATAATTT+TGG	+	Chr8:83594608-83594627	MsG0880047228.01.T01:intron	15.0%
!!	AGCAATCCAAAAAAAAAATT+AGG	-	Chr8:83594946-83594965	None:intergenic	20.0%
!!	ATATATTTCATGGATAATGA+TGG	+	Chr8:83594223-83594242	MsG0880047228.01.T01:intron	20.0%
!!	ATGTACAATTAATAAACCAA+TGG	+	Chr8:83594160-83594179	MsG0880047228.01.T01:intron	20.0%
!!	CAAAAAGTTATCATATAACT+TGG	-	Chr8:83595369-83595388	None:intergenic	20.0%
!!	TGAGTACATAATATATTTCA+TGG	+	Chr8:83594213-83594232	MsG0880047228.01.T01:intron	20.0%
!!	TGTACAATTAATAAACCAAT+GGG	+	Chr8:83594161-83594180	MsG0880047228.01.T01:intron	20.0%
!!	TTTAATAGATGATGTGAAAA+AGG	+	Chr8:83594969-83594988	MsG0880047228.01.T01:intron	20.0%
!!!	ATTTTCATTATGTAATGAAC+TGG	-	Chr8:83594469-83594488	None:intergenic	20.0%
!!!	TGTTTTCATAATATACTTCT+TGG	+	Chr8:83594112-83594131	MsG0880047228.01.T01:intron	20.0%
!!!	TTATTTAGTTTAGCAAGAAA+TGG	-	Chr8:83595289-83595308	None:intergenic	20.0%
!	AAAAAGATTTCACAGAATGA+AGG	+	Chr8:83594993-83595012	MsG0880047228.01.T01:CDS	25.0%
!	AAACAAAAGCAAGAATGTAA+AGG	+	Chr8:83594700-83594719	MsG0880047228.01.T01:CDS	25.0%
!	ATTACTGCACAAAATAAATC+TGG	-	Chr8:83595778-83595797	None:intergenic	25.0%
!	TGTATATTGTATACCATTTG+TGG	-	Chr8:83594561-83594580	None:intergenic	25.0%
!	TTAAACAGTTTAGTGTTTGA+CGG	+	Chr8:83594917-83594936	MsG0880047228.01.T01:intron	25.0%
!	TTCAAACAATACTCTTTGAA+TGG	-	Chr8:83595314-83595333	None:intergenic	25.0%
!	TTCTATAGAGAGATATGAAT+CGG	+	Chr8:83594516-83594535	MsG0880047228.01.T01:CDS	25.0%
!!	AAAAAAAAGGTAAAGTACCT+TGG	-	Chr8:83594844-83594863	None:intergenic	25.0%
!!	AATAAACCAATGGGATTTTT+GGG	+	Chr8:83594170-83594189	MsG0880047228.01.T01:intron	25.0%
!!	AATTTCAGATTTGTTTGAGT+AGG	-	Chr8:83594364-83594383	None:intergenic	25.0%
!!	ATTTCAGATTTGTTTGAGTA+GGG	-	Chr8:83594363-83594382	None:intergenic	25.0%
!!	GTTATATGATAACTTTTTGC+AGG	+	Chr8:83595370-83595389	MsG0880047228.01.T01:intron	25.0%
!!	TAATAAACCAATGGGATTTT+TGG	+	Chr8:83594169-83594188	MsG0880047228.01.T01:intron	25.0%
!!	TATTGTCTGTGCATATATTT+TGG	-	Chr8:83595493-83595512	None:intergenic	25.0%
!!!	TTTGGGGAACAATTTTTTTT+TGG	+	Chr8:83594187-83594206	MsG0880047228.01.T01:intron	25.0%
	AAAATGTGTTTATACCTCAC+AGG	+	Chr8:83594654-83594673	MsG0880047228.01.T01:intron	30.0%
	AAGTTAGTTATGTATCCAAG+TGG	+	Chr8:83594677-83594696	MsG0880047228.01.T01:CDS	30.0%
	AGAATCTTTGATCATGATGA+AGG	+	Chr8:83595146-83595165	MsG0880047228.01.T01:CDS	30.0%
	ATACAAGAATGTAGTTGCAT+TGG	+	Chr8:83595910-83595929	MsG0880047228.01.T01:three_prime_UTR	30.0%
	ATTACATATACATCTAGCCA+TGG	+	Chr8:83594018-83594037	MsG0880047228.01.T01:exon	30.0%
	CACATAGAATAACTAAGCAT+AGG	-	Chr8:83594390-83594409	None:intergenic	30.0%
	GATTAAAGACTTCTCAAAGT+TGG	+	Chr8:83595197-83595216	MsG0880047228.01.T01:CDS	30.0%
	GGATCATATTTCACTATACT+TGG	+	Chr8:83594721-83594740	MsG0880047228.01.T01:CDS	30.0%
	TATTTCACTATACTTGGCAT+TGG	+	Chr8:83594727-83594746	MsG0880047228.01.T01:CDS	30.0%
	TCTATAGAGAGATATGAATC+GGG	+	Chr8:83594517-83594536	MsG0880047228.01.T01:CDS	30.0%
	TCTCTATAGAATTAGTAGTG+AGG	-	Chr8:83594507-83594526	None:intergenic	30.0%
	TGATCAATTTATTCCTCTCA+AGG	+	Chr8:83595029-83595048	MsG0880047228.01.T01:CDS	30.0%
	TTGTAGTTTCCAACATTGAA+TGG	-	Chr8:83595246-83595265	None:intergenic	30.0%
!	AGATCGAAATCTATTTTCGA+GGG	-	Chr8:83594895-83594914	None:intergenic	30.0%
!	ATAAACCAATGGGATTTTTG+GGG	+	Chr8:83594171-83594190	MsG0880047228.01.T01:intron	30.0%
!	GTTACACTTTGTTGTGAAAT+TGG	+	Chr8:83595568-83595587	MsG0880047228.01.T01:intron	30.0%
!	TTTAGTTTAGCAAGAAATGG+AGG	-	Chr8:83595286-83595305	None:intergenic	30.0%
!!!	CGGTCTCCTAATTTTTTTTT+TGG	+	Chr8:83594937-83594956	MsG0880047228.01.T01:intron	30.0%
	AAACTGTATCCCAAAGGAAA+AGG	+	Chr8:83595399-83595418	MsG0880047228.01.T01:CDS	35.0%
	AATTTCACCAGTCAATACCT+GGG	+	Chr8:83595795-83595814	MsG0880047228.01.T01:CDS	35.0%
	AGACTTCTCAAAGTTGGATT+CGG	+	Chr8:83595203-83595222	MsG0880047228.01.T01:CDS	35.0%
	CAGATAAAACTGTATCCCAA+AGG	+	Chr8:83595393-83595412	MsG0880047228.01.T01:CDS	35.0%
	CATTTGCTTTGTAGATGTTG+AGG	+	Chr8:83595833-83595852	MsG0880047228.01.T01:CDS	35.0%
	CTAAAGATGCTACCGAATAA+AGG	-	Chr8:83594075-83594094	None:intergenic	35.0%
	CTATAGAGAGATATGAATCG+GGG	+	Chr8:83594518-83594537	MsG0880047228.01.T01:CDS	35.0%
	GGAAATGAGTTCACAAATGT+AGG	-	Chr8:83593988-83594007	None:intergenic	35.0%
	GTCTACTTGCAGCTAATTAT+TGG	+	Chr8:83595721-83595740	MsG0880047228.01.T01:intron	35.0%
	GTGGATGATATATGTGCATT+TGG	+	Chr8:83595081-83595100	MsG0880047228.01.T01:CDS	35.0%
	TAATTTCACCAGTCAATACC+TGG	+	Chr8:83595794-83595813	MsG0880047228.01.T01:CDS	35.0%
	TACATAACTAACTTCCTGTG+AGG	-	Chr8:83594671-83594690	None:intergenic	35.0%
	TCAATGTTGGAAACTACAAG+TGG	+	Chr8:83595247-83595266	MsG0880047228.01.T01:CDS	35.0%
	TCACAGAATGAAGGTTGAAT+GGG	+	Chr8:83595002-83595021	MsG0880047228.01.T01:CDS	35.0%
	TTCACAGAATGAAGGTTGAA+TGG	+	Chr8:83595001-83595020	MsG0880047228.01.T01:CDS	35.0%
	TTTACATAGATTTCCCAACC+AGG	-	Chr8:83594773-83594792	None:intergenic	35.0%
!	ACTTTGTTGTGAAATTGGTC+AGG	+	Chr8:83595573-83595592	MsG0880047228.01.T01:intron	35.0%
!	AGCAGAAGTCTTTGTTTGTA+GGG	+	Chr8:83595104-83595123	MsG0880047228.01.T01:CDS	35.0%
!	CAGATCGAAATCTATTTTCG+AGG	-	Chr8:83594896-83594915	None:intergenic	35.0%
!	GAGTATTGTTTGAACTCAAC+AGG	+	Chr8:83595320-83595339	MsG0880047228.01.T01:intron	35.0%
!!	TTCTTGCTTTTGTTTCCACT+TGG	-	Chr8:83594695-83594714	None:intergenic	35.0%
!!!	CATTCAGCTGTCATTTTGTA+TGG	-	Chr8:83595610-83595629	None:intergenic	35.0%
!!!	GTGCATATATTTTGGAACCT+GGG	-	Chr8:83595485-83595504	None:intergenic	35.0%
!!!	TGCATATATTTTGGAACCTG+GGG	-	Chr8:83595484-83595503	None:intergenic	35.0%
!!!	TGTGCATATATTTTGGAACC+TGG	-	Chr8:83595486-83595505	None:intergenic	35.0%
	AATCTCCCATACATGCTTGT+AGG	-	Chr8:83595181-83595200	None:intergenic	40.0%
	ACCTTTCCAAAACTGATGCT+TGG	-	Chr8:83595541-83595560	None:intergenic	40.0%
	AGGGAAAACTACACAGGTAA+AGG	+	Chr8:83595123-83595142	MsG0880047228.01.T01:CDS	40.0%
	ATCTCCCATACATGCTTGTA+GGG	-	Chr8:83595180-83595199	None:intergenic	40.0%
	ATTTCACCAGTCAATACCTG+GGG	+	Chr8:83595796-83595815	MsG0880047228.01.T01:CDS	40.0%
	CACAGAATGAAGGTTGAATG+GGG	+	Chr8:83595003-83595022	MsG0880047228.01.T01:CDS	40.0%
	CCTCTCAAGGATTTCAATCT+GGG	+	Chr8:83595042-83595061	MsG0880047228.01.T01:CDS	40.0%
	GACTCTAAGCCATTCAATGT+TGG	+	Chr8:83595234-83595253	MsG0880047228.01.T01:CDS	40.0%
	GATGTTGAGGTTACAGTTCT+TGG	+	Chr8:83595846-83595865	MsG0880047228.01.T01:CDS	40.0%
	GTTCCTCTCTCTCCTTTATT+CGG	+	Chr8:83594060-83594079	MsG0880047228.01.T01:intron	40.0%
	GTTTGTAGGGAAAACTACAC+AGG	+	Chr8:83595117-83595136	MsG0880047228.01.T01:CDS	40.0%
	TCCTCTCAAGGATTTCAATC+TGG	+	Chr8:83595041-83595060	MsG0880047228.01.T01:CDS	40.0%
	TTGTTCCCCAAAAATCCCAT+TGG	-	Chr8:83594179-83594198	None:intergenic	40.0%
!	AGCAATCCAAGCATCAGTTT+TGG	+	Chr8:83595532-83595551	MsG0880047228.01.T01:CDS	40.0%
!	GAGCAGAAGTCTTTGTTTGT+AGG	+	Chr8:83595103-83595122	MsG0880047228.01.T01:CDS	40.0%
!	GAGTCAGTACCTTTTCCTTT+GGG	-	Chr8:83595411-83595430	None:intergenic	40.0%
!	GATTTCAATCTGGGCTCTAA+AGG	+	Chr8:83595051-83595070	MsG0880047228.01.T01:CDS	40.0%
!	GTAAAGTACCTTGGAGAACT+AGG	-	Chr8:83594835-83594854	None:intergenic	40.0%
!!	AAAGGAAAAGGTACTGACTC+GGG	+	Chr8:83595411-83595430	MsG0880047228.01.T01:CDS	40.0%
!!	CAAAGGAAAAGGTACTGACT+CGG	+	Chr8:83595410-83595429	MsG0880047228.01.T01:CDS	40.0%
!!	CTGTTGGGTTAGCTAAAGTT+AGG	-	Chr8:83595455-83595474	None:intergenic	40.0%
!!!	CATATATTTTGGAACCTGGG+GGG	-	Chr8:83595482-83595501	None:intergenic	40.0%
!!!	GCATATATTTTGGAACCTGG+GGG	-	Chr8:83595483-83595502	None:intergenic	40.0%
!!!	TCCAAGCATCAGTTTTGGAA+AGG	+	Chr8:83595537-83595556	MsG0880047228.01.T01:CDS	40.0%
	AAGCTCACTTCATCCTGGTT+GGG	+	Chr8:83594757-83594776	MsG0880047228.01.T01:CDS	45.0%
	ACCGTCATCTTTGGTATCCA+TGG	-	Chr8:83594038-83594057	None:intergenic	45.0%
	AGTGCCTCAAGTCATGAACA+TGG	+	Chr8:83595747-83595766	MsG0880047228.01.T01:CDS	45.0%
	CCCAGATTGAAATCCTTGAG+AGG	-	Chr8:83595045-83595064	None:intergenic	45.0%
	CTACCGAATAAAGGAGAGAG+AGG	-	Chr8:83594066-83594085	None:intergenic	45.0%
	GAATCAAGCTCACTTCATCC+TGG	+	Chr8:83594752-83594771	MsG0880047228.01.T01:CDS	45.0%
	GAGGAACAAACCGTCATCTT+TGG	-	Chr8:83594047-83594066	None:intergenic	45.0%
	GCCATGGATACCAAAGATGA+CGG	+	Chr8:83594034-83594053	MsG0880047228.01.T01:exon	45.0%
	TCTCCCATACATGCTTGTAG+GGG	-	Chr8:83595179-83595198	None:intergenic	45.0%
	TGAGGTTACAGTTCTTGGAG+TGG	+	Chr8:83595851-83595870	MsG0880047228.01.T01:CDS	45.0%
	TGTGCCATGTTCATGACTTG+AGG	-	Chr8:83595754-83595773	None:intergenic	45.0%
!	ATACCTGGGGTATCTAGTGA+AGG	+	Chr8:83595809-83595828	MsG0880047228.01.T01:CDS	45.0%
!	ATTTGTTTGAGTAGGGCGTC+TGG	-	Chr8:83594356-83594375	None:intergenic	45.0%
!	CGAGTCAGTACCTTTTCCTT+TGG	-	Chr8:83595412-83595431	None:intergenic	45.0%
!	GACAACTACCTAGTTCTCCA+AGG	+	Chr8:83594824-83594843	MsG0880047228.01.T01:CDS	45.0%
!!	GGGCTCTAAAGGTTATCTAG+TGG	+	Chr8:83595062-83595081	MsG0880047228.01.T01:CDS	45.0%
!!	TAGATACCCCAGGTATTGAC+TGG	-	Chr8:83595805-83595824	None:intergenic	45.0%
!!	TGAATATATATTAAAAAAAA+AGG	-	Chr8:83594857-83594876	None:intergenic	5.0%
	CAAGCTCACTTCATCCTGGT+TGG	+	Chr8:83594756-83594775	MsG0880047228.01.T01:CDS	50.0%
	CCATACATGCTTGTAGGGGA+GGG	-	Chr8:83595175-83595194	None:intergenic	50.0%
	CCTCCCCTACAAGCATGTAT+GGG	+	Chr8:83595173-83595192	MsG0880047228.01.T01:CDS	50.0%
	TGTCCTTCACTAGATACCCC+AGG	-	Chr8:83595815-83595834	None:intergenic	50.0%
	TTGAAGCTGGAGGCCACAAA+TGG	+	Chr8:83594545-83594564	MsG0880047228.01.T01:CDS	50.0%
!	GAATCGGGGAAGTTTGAAGC+TGG	+	Chr8:83594532-83594551	MsG0880047228.01.T01:CDS	50.0%
	CCCATACATGCTTGTAGGGG+AGG	-	Chr8:83595176-83595195	None:intergenic	55.0%
	CCCTCCCCTACAAGCATGTA+TGG	+	Chr8:83595172-83595191	MsG0880047228.01.T01:CDS	55.0%
!	AACCTGGGGGGATTGCTGTT+GGG	-	Chr8:83595470-83595489	None:intergenic	55.0%
!	TCGGGGAAGTTTGAAGCTGG+AGG	+	Chr8:83594535-83594554	MsG0880047228.01.T01:CDS	55.0%
	AACCCAACAGCAATCCCCCC+AGG	+	Chr8:83595465-83595484	MsG0880047228.01.T01:CDS	60.0%
	GAACCTGGGGGGATTGCTGT+TGG	-	Chr8:83595471-83595490	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	83593981	83595970	83593981	ID=MsG0880047228.01;Name=MsG0880047228.01
Chr8	mRNA	83593981	83595970	83593981	ID=MsG0880047228.01.T01;Parent=MsG0880047228.01;Name=MsG0880047228.01.T01;_AED=0.34;_eAED=0.34;_QI=56\|1\|1\|1\|0.8\|0.66\|6\|80\|311
Chr8	exon	83593981	83594055	83593981	ID=MsG0880047228.01.T01:exon:20768;Parent=MsG0880047228.01.T01
Chr8	exon	83594437	83594566	83594437	ID=MsG0880047228.01.T01:exon:20769;Parent=MsG0880047228.01.T01
Chr8	exon	83594676	83594845	83594676	ID=MsG0880047228.01.T01:exon:20770;Parent=MsG0880047228.01.T01
Chr8	exon	83594977	83595268	83594977	ID=MsG0880047228.01.T01:exon:20771;Parent=MsG0880047228.01.T01
Chr8	exon	83595392	83595558	83595392	ID=MsG0880047228.01.T01:exon:20772;Parent=MsG0880047228.01.T01
Chr8	exon	83595733	83595970	83595733	ID=MsG0880047228.01.T01:exon:20773;Parent=MsG0880047228.01.T01
Chr8	five_prime_UTR	83593981	83594036	83593981	ID=MsG0880047228.01.T01:five_prime_utr;Parent=MsG0880047228.01.T01
Chr8	CDS	83594037	83594055	83594037	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	CDS	83594437	83594566	83594437	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	CDS	83594676	83594845	83594676	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	CDS	83594977	83595268	83594977	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	CDS	83595392	83595558	83595392	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	CDS	83595733	83595890	83595733	ID=MsG0880047228.01.T01:cds;Parent=MsG0880047228.01.T01
Chr8	three_prime_UTR	83595891	83595970	83595891	ID=MsG0880047228.01.T01:three_prime_utr;Parent=MsG0880047228.01.T01

Gene Sequence

>MsG0880047228.01.T01

ATGGATACCAAAGATGACGGTATTTCTAGATCTATAGTAGAAGCTTCGCCAGTTCATTACATAATGAAAATACAATCATTTTCACTCCTCACTACTAATTCTATAGAGAGATATGAATCGGGGAAGTTTGAAGCTGGAGGCCACAAATGGAAGTTAGTTATGTATCCAAGTGGAAACAAAAGCAAGAATGTAAAGGATCATATTTCACTATACTTGGCATTGGAAGAATCAAGCTCACTTCATCCTGGTTGGGAAATCTATGTAAATTTTAAATTGTTTTTATATGATCACAACAATGACAACTACCTAGTTCTCCAAGATGATGTGAAAAAGGAAAAAAGATTTCACAGAATGAAGGTTGAATGGGGATTTGATCAATTTATTCCTCTCAAGGATTTCAATCTGGGCTCTAAAGGTTATCTAGTGGATGATATATGTGCATTTGGAGCAGAAGTCTTTGTTTGTAGGGAAAACTACACAGGTAAAGGAGAATCTTTGATCATGATGAAGGATGCCCTCCCCTACAAGCATGTATGGGAGATTAAAGACTTCTCAAAGTTGGATTCGGAGTGTTGTGACTCTAAGCCATTCAATGTTGGAAACTACAAGTGGCAGATAAAACTGTATCCCAAAGGAAAAGGTACTGACTCGGGCAGATATCTTGCTCTGTACCTAACTTTAGCTAACCCAACAGCAATCCCCCCAGGTTCCAAAATATATGCACAGACAATATTACGCATACTTGATCAAAAGCAATCCAAGCATCAGTTTTGGAAAGCTAATTATTGGTTTAGTGCCTCAAGTCATGAACATGGCACATCCAGATTTATTTTGTGCAGTAATTTCACCAGTCAATACCTGGGGTATCTAGTGAAGGACATTTGCTTTGTAGATGTTGAGGTTACAGTTCTTGGAGTGGTTGATGCATTGTCTTGA

Protein sequence

>MsG0880047228.01.T01

MDTKDDGISRSIVEASPVHYIMKIQSFSLLTTNSIERYESGKFEAGGHKWKLVMYPSGNKSKNVKDHISLYLALEESSSLHPGWEIYVNFKLFLYDHNNDNYLVLQDDVKKEKRFHRMKVEWGFDQFIPLKDFNLGSKGYLVDDICAFGAEVFVCRENYTGKGESLIMMKDALPYKHVWEIKDFSKLDSECCDSKPFNVGNYKWQIKLYPKGKGTDSGRYLALYLTLANPTAIPPGSKIYAQTILRILDQKQSKHQFWKANYWFSASSHEHGTSRFILCSNFTSQYLGYLVKDICFVDVEVTVLGVVDALS*