AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045943.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045943.01.T01 MTR_4g095280 88.462 208 18 3 1 203 1 207 5.47e-75 225
MsG0880045943.01.T01 MTR_5g016250 49.393 247 58 10 1 203 1 224 8.81e-20 84.0
MsG0880045943.01.T02 MTR_4g095280 83.168 202 23 5 1 192 1 201 2.02e-92 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045943.01.T01 AT4G37450 58.333 72 22 3 1 64 1 72 8.83e-13 65.1
MsG0880045943.01.T01 AT4G37450 58.333 72 22 3 1 64 70 141 1.80e-12 65.1
MsG0880045943.01.T02 AT4G37450 58.333 72 22 3 1 64 1 72 5.56e-13 65.1
MsG0880045943.01.T02 AT4G37450 58.333 72 22 3 1 64 70 141 1.23e-12 65.1

Find 99 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTTTCAACTTTCAATA+TGG 0.214473 8:-66753448 MsG0880045943.01.T01:exon
CCCTTGCTTGGTGCTGGAGC+TGG 0.249954 8:+66753054 MsG0880045944.01.T01:CDS
TTCTTTCCCTTGCTTGGTGC+TGG 0.263309 8:+66753048 MsG0880045944.01.T01:CDS
GGTGTTGTTGGTGTGGTTGT+AGG 0.304465 8:+66753285 MsG0880045944.01.T01:CDS
GGTGCTCCTGCTGGTGGTGC+TGG 0.331916 8:+66752976 MsG0880045944.01.T01:CDS
AGCAGCTGGAGGAGATGATT+TGG 0.353606 8:+66753245 MsG0880045944.01.T01:CDS
TTGTTCTTCTTTCCCTTGCT+TGG 0.362413 8:+66753042 MsG0880045944.01.T01:CDS
GGAGGTTTGGTTGCCGGAGC+AGG 0.362636 8:+66753219 MsG0880045944.01.T01:CDS
GTGGTGGCGGCCGGAGTTGA+TGG 0.392958 8:+66753336 MsG0880045944.01.T01:CDS
CACCACTCCTGTTGCATCTC+CGG 0.398121 8:-66753319 MsG0880045943.01.T01:CDS
AGAGTGGAGCAGAAACTATC+AGG 0.404075 8:-66752056 MsG0880045943.01.T01:CDS
GCTGCCGGAGGTTTGGTTGC+CGG 0.408047 8:+66753213 MsG0880045944.01.T01:CDS
GCACCACCAGCAGGAGCACC+TGG 0.414679 8:-66752973 MsG0880045943.01.T01:CDS
CCTGCATCTTCGCTTGGTCC+AGG 0.415878 8:+66752955 MsG0880045944.01.T01:CDS
GCTGGTGGTGGAGTGCTGAC+TGG 0.421414 8:+66753171 MsG0880045944.01.T01:CDS
GGTGGTGAGCTGACTGGAGC+AGG 0.426318 8:+66753120 MsG0880045944.01.T01:CDS
GCCGGAGCAGGTGCAGCAGC+TGG 0.433970 8:+66753231 MsG0880045944.01.T01:CDS
AGAGAAAGAGTGAGTGAGAG+AGG 0.435994 8:+66753511 None:intergenic
AGCACCATCACCAGCGTTGT+TGG 0.436518 8:-66753004 MsG0880045943.01.T01:CDS
GCACCATCACCAGCGTTGTT+GGG 0.436941 8:-66753003 MsG0880045943.01.T01:CDS
GCAGCTGGAGGAGATGATTT+GGG 0.439226 8:+66753246 MsG0880045944.01.T01:CDS
AGGTGACTGGCCTCCGACGC+CGG 0.439946 8:+66753380 MsG0880045944.01.T01:CDS
GGTGCTGGAGCTGGAACCGC+TGG 0.440384 8:+66753063 MsG0880045944.01.T01:CDS
AAGATTTACCTCATCGTTCG+CGG 0.444163 8:+66752912 MsG0880045944.01.T01:intron
GCTCCTGCTGGTGGTGCTGG+TGG 0.457667 8:+66752979 MsG0880045944.01.T01:CDS
TGCATTATCGTCGCCGGCGT+CGG 0.458092 8:-66753393 MsG0880045943.01.T01:CDS
TCCAGCTGCTGCACCTGCTC+CGG 0.458206 8:-66753232 MsG0880045943.01.T01:CDS
CTTGGTCCAGGTGCTCCTGC+TGG 0.461592 8:+66752967 MsG0880045944.01.T01:CDS
TGGTGATGCTGCCGGAGGTT+TGG 0.463953 8:+66753206 MsG0880045944.01.T01:CDS
TTGGGAGAAGCAACGGGAGC+TGG 0.468676 8:+66753264 MsG0880045944.01.T01:CDS
GAGCTGACTGGAGCAGGTGT+GGG 0.472900 8:+66753126 MsG0880045944.01.T01:CDS
TTGGTGCAGCGGAAGGTGAC+TGG 0.475188 8:+66753367 MsG0880045944.01.T01:CDS
ACAGGAACTGGTGCTGGTGG+TGG 0.475601 8:+66753159 MsG0880045944.01.T01:CDS
ACTGCTGGTGGTGAGCTGAC+TGG 0.475791 8:+66753114 MsG0880045944.01.T01:CDS
AGGCTTAGCTTTGAGCTGGA+TGG 0.476021 8:-66752016 MsG0880045943.01.T01:CDS
GGTGGTGATTTGACAGGAAC+TGG 0.477818 8:+66753147 MsG0880045944.01.T01:CDS
TTCGCGGTAGAAGCGGGTCC+AGG 0.478864 8:+66752928 MsG0880045944.01.T01:intron
GGTGCTCCTGCATCTTCGCT+TGG 0.481476 8:+66752949 MsG0880045944.01.T01:CDS
AGCGAAGATGCAGGAGCACC+TGG 0.485174 8:-66752946 MsG0880045943.01.T01:CDS
TGAGCTGACTGGAGCAGGTG+TGG 0.488282 8:+66753125 MsG0880045944.01.T01:CDS
CTCATCGTTCGCGGTAGAAG+CGG 0.489228 8:+66752921 MsG0880045944.01.T01:intron
GCAACGGGAGCTGGTGTTGT+TGG 0.490912 8:+66753273 MsG0880045944.01.T01:CDS
GATGATTTGGGAGAAGCAAC+GGG 0.491381 8:+66753258 MsG0880045944.01.T01:CDS
GCTGCTACCGGAGATGCAAC+AGG 0.501881 8:+66753312 MsG0880045944.01.T01:CDS
GCAACAGGAGTGGTGGCGGC+CGG 0.507920 8:+66753327 MsG0880045944.01.T01:CDS
TCCAGCTCCAGCACCAAGCA+AGG 0.510907 8:-66753055 MsG0880045943.01.T01:CDS
TCATCGTTCGCGGTAGAAGC+GGG 0.516917 8:+66752922 MsG0880045944.01.T01:intron
GGAGTTGATGGTGAGGTGGT+TGG 0.517435 8:+66753348 MsG0880045944.01.T01:CDS
GTGGTGGTTGGAGCAGCCAC+AGG 0.519021 8:+66753087 MsG0880045944.01.T01:CDS
CCTGGACCAAGCGAAGATGC+AGG 0.520602 8:-66752955 MsG0880045943.01.T01:CDS
GGTCCAGGTGCTCCTGCTGG+TGG 0.523649 8:+66752970 MsG0880045944.01.T01:CDS
AGAGTGTGATAATATGATCA+AGG 0.530650 8:+66751960 MsG0880045944.01.T01:intron
AGCTGGAACCGCTGGTGTGG+TGG 0.532724 8:+66753071 MsG0880045944.01.T01:CDS
GGAACCGCTGGTGTGGTGGT+TGG 0.535849 8:+66753075 MsG0880045944.01.T01:CDS
AACCACCTCACCATCAACTC+CGG 0.540937 8:-66753346 MsG0880045943.01.T01:CDS
AGATGATTTGGGAGAAGCAA+CGG 0.541525 8:+66753257 MsG0880045944.01.T01:CDS
GAGGTGGTTGGTGCAGCGGA+AGG 0.541625 8:+66753360 MsG0880045944.01.T01:CDS
AACAACGCTGGTGATGGTGC+TGG 0.542731 8:+66753006 MsG0880045944.01.T01:CDS
AGATGCAACAGGAGTGGTGG+CGG 0.543847 8:+66753323 MsG0880045944.01.T01:CDS
GTTGTTGGTGTGGTTGTAGG+AGG 0.547446 8:+66753288 MsG0880045944.01.T01:CDS
GAGAAAGAGTGAGTGAGAGA+GGG 0.548946 8:+66753512 None:intergenic
TAGGAGGCTTAGCTTTGAGC+TGG 0.549247 8:-66752020 MsG0880045943.01.T01:CDS
TGGAGCTGGAACCGCTGGTG+TGG 0.549390 8:+66753068 MsG0880045944.01.T01:CDS
GGTCCACCAGCACCACCAGC+AGG 0.553644 8:-66752982 MsG0880045943.01.T01:CDS
GGAGCAGGTGCAGCAGCTGG+AGG 0.554589 8:+66753234 MsG0880045944.01.T01:CDS
TTCATCTGCATTATCGTCGC+CGG 0.560042 8:-66753399 MsG0880045943.01.T01:CDS
TGCTCCGGCAACCAAACCTC+CGG 0.560313 8:-66753217 MsG0880045943.01.T01:CDS
CTGACTGGAGCAGGTGTGGG+TGG 0.564849 8:+66753129 MsG0880045944.01.T01:CDS
GGCCGGAGTTGATGGTGAGG+TGG 0.565131 8:+66753344 MsG0880045944.01.T01:CDS
ACCTCCGGCAGCATCACCAC+CGG 0.566788 8:-66753202 MsG0880045943.01.T01:CDS
GGACCCAACAACGCTGGTGA+TGG 0.568484 8:+66753000 MsG0880045944.01.T01:CDS
GATTTGACAGGAACTGGTGC+TGG 0.568714 8:+66753153 MsG0880045944.01.T01:CDS
GTGCTGACTGGTGTCACCGC+CGG 0.572577 8:+66753183 MsG0880045944.01.T01:CDS
ACCGCCGGTGGTGATGCTGC+CGG 0.575279 8:+66753198 MsG0880045944.01.T01:CDS
GCAGCCACAGGTGTTACTGC+TGG 0.577094 8:+66753099 MsG0880045944.01.T01:CDS
ATTATCGTCGCCGGCGTCGG+AGG 0.579627 8:-66753390 MsG0880045943.01.T01:CDS
CGGAGATGCAACAGGAGTGG+TGG 0.583940 8:+66753320 MsG0880045944.01.T01:CDS
GTTGTAGGAGGTGCTGCTAC+CGG 0.584827 8:+66753300 MsG0880045944.01.T01:CDS
ATAGTATCATAGATGTCTCA+AGG 0.606965 8:+66751839 MsG0880045944.01.T01:intron
TGCTCCAACCACCACACCAG+CGG 0.608500 8:-66753079 MsG0880045943.01.T01:CDS
TGGTGAGGTGGTTGGTGCAG+CGG 0.610327 8:+66753356 MsG0880045944.01.T01:CDS
GGGAGCTGGTGTTGTTGGTG+TGG 0.611766 8:+66753278 MsG0880045944.01.T01:CDS
TTGACAGGAACTGGTGCTGG+TGG 0.617477 8:+66753156 MsG0880045944.01.T01:CDS
CGATAATGCAGATGAATGCG+AGG 0.621043 8:+66753406 MsG0880045944.01.T01:CDS
GCCGGTGGTGATGCTGCCGG+AGG 0.626872 8:+66753201 MsG0880045944.01.T01:CDS
ATCAGGAGCTTGAAGATGCT+AGG 0.627021 8:-66752039 MsG0880045943.01.T01:CDS
CTGACTGGTGTCACCGCCGG+TGG 0.627806 8:+66753186 MsG0880045944.01.T01:CDS
GGCGGCCGGAGTTGATGGTG+AGG 0.629145 8:+66753341 MsG0880045944.01.T01:CDS
TACCGGAGATGCAACAGGAG+TGG 0.639684 8:+66753317 MsG0880045944.01.T01:CDS
GGAAAGAAGAACAAGAAGCA+CGG 0.641441 8:-66753033 MsG0880045943.01.T01:CDS
CCAGCTCCAGCACCAAGCAA+GGG 0.643686 8:-66753054 MsG0880045943.01.T01:CDS
GGTGTGGGTGGTGATTTGAC+AGG 0.655827 8:+66753141 MsG0880045944.01.T01:CDS
GCTGGTGGACCCAACAACGC+TGG 0.656094 8:+66752994 MsG0880045944.01.T01:CDS
CGCTTCTACCGCGAACGATG+AGG 0.660407 8:-66752920 MsG0880045943.01.T01:intron
GCCACAGGTGTTACTGCTGG+TGG 0.660434 8:+66753102 MsG0880045944.01.T01:CDS
TCCGGCAGCATCACCACCGG+CGG 0.672479 8:-66753199 MsG0880045943.01.T01:CDS
AGGAGCTTGAAGATGCTAGG+AGG 0.675654 8:-66752036 MsG0880045943.01.T01:CDS
GATAATGCAGATGAATGCGA+GGG 0.678738 8:+66753407 MsG0880045944.01.T01:CDS
ACCACCAGCAGTAACACCTG+TGG 0.703069 8:-66753103 MsG0880045943.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTTTCTTTTTAAAATAA+AGG + Chr8:66752837-66752856 MsG0880045944.01.T01:intron 10.0%
!! AAATAAGGTTATAAAGATAA+AGG - Chr8:66752740-66752759 MsG0880045943.01.T01:intron 15.0%
!! TAAGAATAAAAAAAAAAGGA+TGG + Chr8:66753376-66753395 MsG0880045944.01.T01:CDS 15.0%
!!! AAATATATTGTTTTCATACA+TGG - Chr8:66752886-66752905 MsG0880045943.01.T01:intron 15.0%
!! AATGATGGTTTAATGTTAAT+CGG - Chr8:66753177-66753196 MsG0880045943.01.T01:CDS 20.0%
!! GTATGATTATAAAACAATGA+TGG - Chr8:66753162-66753181 MsG0880045943.01.T01:CDS 20.0%
!! TAGAAAATATGTTCATAGTT+TGG - Chr8:66752783-66752802 MsG0880045943.01.T01:intron 20.0%
!! TGAATAGTATATGTCTATTT+AGG + Chr8:66752862-66752881 MsG0880045944.01.T01:intron 20.0%
!!! AAGAAATTTCAAGATTTGTT+TGG + Chr8:66753204-66753223 MsG0880045944.01.T01:CDS 20.0%
!!! ATTTTATTCATATTTGGATC+TGG + Chr8:66752715-66752734 MsG0880045944.01.T01:intron 20.0%
!!! TTATTTTTTTGAAAATGCTG+TGG - Chr8:66753491-66753510 MsG0880045943.01.T01:five_prime_UTR 20.0%
! ACCATAAAAAGAAGAGTAAT+TGG - Chr8:66752627-66752646 MsG0880045943.01.T01:intron 25.0%
! ATAGTTAACATAGTACAATG+TGG - Chr8:66752442-66752461 MsG0880045943.01.T01:intron 25.0%
! CAAACTTTCAACTTTCAATA+TGG - Chr8:66751848-66751867 MsG0880045943.01.T01:three_prime_UTR 25.0%
! CTAACCAAAATCTAAGTTTA+AGG - Chr8:66752526-66752545 MsG0880045943.01.T01:intron 25.0%
!! ACCAATTACTCTTCTTTTTA+TGG + Chr8:66752631-66752650 MsG0880045944.01.T01:intron 25.0%
!! TTTCATTTTCAGACCTAATA+AGG + Chr8:66752596-66752615 MsG0880045944.01.T01:intron 25.0%
!!! ATGAAATGATGTTAGTTTTC+TGG - Chr8:66753094-66753113 MsG0880045943.01.T01:CDS 25.0%
!!! TCTTATTTTGGCTATCTTAT+GGG - Chr8:66753391-66753410 MsG0880045943.01.T01:CDS 25.0%
!!! TGTGGTCATTATTTTATTCT+TGG - Chr8:66753509-66753528 MsG0880045943.01.T01:five_prime_UTR 25.0%
!!! TTCTTATTTTGGCTATCTTA+TGG - Chr8:66753390-66753409 MsG0880045943.01.T01:CDS 25.0%
AGAGTGTGATAATATGATCA+AGG + Chr8:66753339-66753358 MsG0880045944.01.T01:CDS 30.0%
ATAGTATCATAGATGTCTCA+AGG + Chr8:66753460-66753479 MsG0880045944.01.T01:CDS 30.0%
ATTAACAGTTACTGATGATC+TGG - Chr8:66752927-66752946 MsG0880045943.01.T01:CDS 30.0%
TATTCATATTTGGATCTGGA+TGG + Chr8:66752711-66752730 MsG0880045944.01.T01:intron 30.0%
TTAACAGTTACTGATGATCT+GGG - Chr8:66752928-66752947 MsG0880045943.01.T01:CDS 30.0%
! AAAGATAAAGGTGTACTATC+TGG - Chr8:66752752-66752771 MsG0880045943.01.T01:intron 30.0%
! CACACCTTAAACTTAGATTT+TGG + Chr8:66752533-66752552 MsG0880045944.01.T01:intron 30.0%
!! GTTGTTTTATTCTTCTAGTC+TGG - Chr8:66753305-66753324 MsG0880045943.01.T01:CDS 30.0%
!!! TGTTCCATTTTTTTTCAGAG+TGG - Chr8:66753224-66753243 MsG0880045943.01.T01:CDS 30.0%
! TGCTCCACTCTGAAAAAAAA+TGG + Chr8:66753231-66753250 MsG0880045944.01.T01:CDS 35.0%
AAGATTTACCTCATCGTTCG+CGG + Chr8:66752387-66752406 MsG0880045944.01.T01:intron 40.0%
GATAATGCAGATGAATGCGA+GGG + Chr8:66751892-66751911 MsG0880045944.01.T01:intron 40.0%
GGAAAGAAGAACAAGAAGCA+CGG - Chr8:66752263-66752282 MsG0880045943.01.T01:intron 40.0%
TTGTTCTTCTTTCCCTTGCT+TGG + Chr8:66752257-66752276 MsG0880045944.01.T01:intron 40.0%
! AGATGATTTGGGAGAAGCAA+CGG + Chr8:66752042-66752061 MsG0880045944.01.T01:intron 40.0%
!!! GAGCTGTCGTTTTTGTTTTC+TGG - Chr8:66752673-66752692 MsG0880045943.01.T01:intron 40.0%
AGAGTGGAGCAGAAACTATC+AGG - Chr8:66753240-66753259 MsG0880045943.01.T01:CDS 45.0%
ATCAGGAGCTTGAAGATGCT+AGG - Chr8:66753257-66753276 MsG0880045943.01.T01:CDS 45.0%
CGATAATGCAGATGAATGCG+AGG + Chr8:66751893-66751912 MsG0880045944.01.T01:intron 45.0%
GGTGTAGGAGACACCTTATT+AGG - Chr8:66752580-66752599 MsG0880045943.01.T01:intron 45.0%
TTCATCTGCATTATCGTCGC+CGG - Chr8:66751897-66751916 MsG0880045943.01.T01:three_prime_UTR 45.0%
! GATGATTTGGGAGAAGCAAC+GGG + Chr8:66752041-66752060 MsG0880045944.01.T01:intron 45.0%
!! AAAATAAGAATAAAAAAAAA+AGG + Chr8:66753380-66753399 MsG0880045944.01.T01:CDS 5.0%
!! TGAATAAAATAAATAAAATA+AGG - Chr8:66752725-66752744 MsG0880045943.01.T01:intron 5.0%
!!! GAAAATAATTTTATTTATAT+TGG + Chr8:66753046-66753065 MsG0880045944.01.T01:CDS 5.0%
!!! TTATTTATTTTATTCATATT+TGG + Chr8:66752721-66752740 MsG0880045944.01.T01:intron 5.0%
!!! TTTTTTTTTTATTCTTATTT+TGG - Chr8:66753379-66753398 MsG0880045943.01.T01:CDS 5.0%
AACCACCTCACCATCAACTC+CGG - Chr8:66751950-66751969 MsG0880045943.01.T01:three_prime_UTR 50.0%
AGCAGCTGGAGGAGATGATT+TGG + Chr8:66752054-66752073 MsG0880045944.01.T01:intron 50.0%
AGGAGCTTGAAGATGCTAGG+AGG - Chr8:66753260-66753279 MsG0880045943.01.T01:CDS 50.0%
GATTTGACAGGAACTGGTGC+TGG + Chr8:66752146-66752165 MsG0880045944.01.T01:intron 50.0%
GCAGCTGGAGGAGATGATTT+GGG + Chr8:66752053-66752072 MsG0880045944.01.T01:intron 50.0%
GGTGGTGATTTGACAGGAAC+TGG + Chr8:66752152-66752171 MsG0880045944.01.T01:intron 50.0%
GTTGTTGGTGTGGTTGTAGG+AGG + Chr8:66752011-66752030 MsG0880045944.01.T01:intron 50.0%
!! AGGCTTAGCTTTGAGCTGGA+TGG - Chr8:66753280-66753299 MsG0880045943.01.T01:CDS 50.0%
!! GGTGTTGTTGGTGTGGTTGT+AGG + Chr8:66752014-66752033 MsG0880045944.01.T01:intron 50.0%
!! TAGGAGGCTTAGCTTTGAGC+TGG - Chr8:66753276-66753295 MsG0880045943.01.T01:CDS 50.0%
!! TTCTTTCCCTTGCTTGGTGC+TGG + Chr8:66752251-66752270 MsG0880045944.01.T01:intron 50.0%
ACCACCAGCAGTAACACCTG+TGG - Chr8:66752193-66752212 MsG0880045943.01.T01:intron 55.0%
AGATGCAACAGGAGTGGTGG+CGG + Chr8:66751976-66751995 MsG0880045944.01.T01:intron 55.0%
AGCACCATCACCAGCGTTGT+TGG - Chr8:66752292-66752311 MsG0880045943.01.T01:intron 55.0%
CACCACTCCTGTTGCATCTC+CGG - Chr8:66751977-66751996 MsG0880045943.01.T01:exon 55.0%
CTCATCGTTCGCGGTAGAAG+CGG + Chr8:66752378-66752397 MsG0880045944.01.T01:intron 55.0%
GCACCATCACCAGCGTTGTT+GGG - Chr8:66752293-66752312 MsG0880045943.01.T01:intron 55.0%
GGTGTGGGTGGTGATTTGAC+AGG + Chr8:66752158-66752177 MsG0880045944.01.T01:intron 55.0%
TACACCGAACCCACCGTCAA+TGG + Chr8:66752566-66752585 MsG0880045944.01.T01:intron 55.0%
TACCGGAGATGCAACAGGAG+TGG + Chr8:66751982-66752001 MsG0880045944.01.T01:intron 55.0%
TCATCGTTCGCGGTAGAAGC+GGG + Chr8:66752377-66752396 MsG0880045944.01.T01:intron 55.0%
! TTGACAGGAACTGGTGCTGG+TGG + Chr8:66752143-66752162 MsG0880045944.01.T01:intron 55.0%
!! AACAACGCTGGTGATGGTGC+TGG + Chr8:66752293-66752312 MsG0880045944.01.T01:intron 55.0%
!! ATTGACGGTGGGTTCGGTGT+AGG - Chr8:66752565-66752584 MsG0880045943.01.T01:intron 55.0%
!! GGAGTTGATGGTGAGGTGGT+TGG + Chr8:66751951-66751970 MsG0880045944.01.T01:intron 55.0%
!! GTTGTAGGAGGTGCTGCTAC+CGG + Chr8:66751999-66752018 MsG0880045944.01.T01:intron 55.0%
ACCGAACCCACCGTCAATGG+TGG + Chr8:66752563-66752582 MsG0880045944.01.T01:intron 60.0%
CCAGCTCCAGCACCAAGCAA+GGG - Chr8:66752242-66752261 MsG0880045943.01.T01:intron 60.0%
CCTGCATCTTCGCTTGGTCC+AGG + Chr8:66752344-66752363 MsG0880045944.01.T01:intron 60.0%
CCTGGACCAAGCGAAGATGC+AGG - Chr8:66752341-66752360 MsG0880045943.01.T01:intron 60.0%
CGCTTCTACCGCGAACGATG+AGG - Chr8:66752376-66752395 MsG0880045943.01.T01:intron 60.0%
CGGAGATGCAACAGGAGTGG+TGG + Chr8:66751979-66751998 MsG0880045944.01.T01:intron 60.0%
GAGCTGACTGGAGCAGGTGT+GGG + Chr8:66752173-66752192 MsG0880045944.01.T01:intron 60.0%
GCTGCTACCGGAGATGCAAC+AGG + Chr8:66751987-66752006 MsG0880045944.01.T01:intron 60.0%
GGACCCAACAACGCTGGTGA+TGG + Chr8:66752299-66752318 MsG0880045944.01.T01:intron 60.0%
GGTGCTCCTGCATCTTCGCT+TGG + Chr8:66752350-66752369 MsG0880045944.01.T01:intron 60.0%
TCCAGCTCCAGCACCAAGCA+AGG - Chr8:66752241-66752260 MsG0880045943.01.T01:intron 60.0%
TGAGCTGACTGGAGCAGGTG+TGG + Chr8:66752174-66752193 MsG0880045944.01.T01:intron 60.0%
TGCATTATCGTCGCCGGCGT+CGG - Chr8:66751903-66751922 MsG0880045943.01.T01:three_prime_UTR 60.0%
TGCTCCAACCACCACACCAG+CGG - Chr8:66752217-66752236 MsG0880045943.01.T01:intron 60.0%
TGCTCCGGCAACCAAACCTC+CGG - Chr8:66752079-66752098 MsG0880045943.01.T01:intron 60.0%
TTGGGAGAAGCAACGGGAGC+TGG + Chr8:66752035-66752054 MsG0880045944.01.T01:intron 60.0%
TTGGTGCAGCGGAAGGTGAC+TGG + Chr8:66751932-66751951 MsG0880045944.01.T01:intron 60.0%
! ACTGCTGGTGGTGAGCTGAC+TGG + Chr8:66752185-66752204 MsG0880045944.01.T01:intron 60.0%
! GCCACCATTGACGGTGGGTT+CGG - Chr8:66752559-66752578 MsG0880045943.01.T01:intron 60.0%
! TGGTGAGGTGGTTGGTGCAG+CGG + Chr8:66751943-66751962 MsG0880045944.01.T01:intron 60.0%
! TGGTGATGCTGCCGGAGGTT+TGG + Chr8:66752093-66752112 MsG0880045944.01.T01:intron 60.0%
!! ACAGGAACTGGTGCTGGTGG+TGG + Chr8:66752140-66752159 MsG0880045944.01.T01:intron 60.0%
!! AGCGAAGATGCAGGAGCACC+TGG - Chr8:66752350-66752369 MsG0880045943.01.T01:intron 60.0%
!! GCAACGGGAGCTGGTGTTGT+TGG + Chr8:66752026-66752045 MsG0880045944.01.T01:intron 60.0%
!! GCAGCCACAGGTGTTACTGC+TGG + Chr8:66752200-66752219 MsG0880045944.01.T01:intron 60.0%
!! GCCACAGGTGTTACTGCTGG+TGG + Chr8:66752197-66752216 MsG0880045944.01.T01:intron 60.0%
!! GGGAGCTGGTGTTGTTGGTG+TGG + Chr8:66752021-66752040 MsG0880045944.01.T01:intron 60.0%
ACCTCCGGCAGCATCACCAC+CGG - Chr8:66752094-66752113 MsG0880045943.01.T01:intron 65.0%
AGCTGGAACCGCTGGTGTGG+TGG + Chr8:66752228-66752247 MsG0880045944.01.T01:intron 65.0%
CACGCGCCACCATTGACGGT+GGG - Chr8:66752554-66752573 MsG0880045943.01.T01:intron 65.0%
CTGACTGGAGCAGGTGTGGG+TGG + Chr8:66752170-66752189 MsG0880045944.01.T01:intron 65.0%
CTTGGTCCAGGTGCTCCTGC+TGG + Chr8:66752332-66752351 MsG0880045944.01.T01:intron 65.0%
GGAACCGCTGGTGTGGTGGT+TGG + Chr8:66752224-66752243 MsG0880045944.01.T01:intron 65.0%
GGTGGTGAGCTGACTGGAGC+AGG + Chr8:66752179-66752198 MsG0880045944.01.T01:intron 65.0%
GTGCCACGCGCCACCATTGA+CGG - Chr8:66752550-66752569 MsG0880045943.01.T01:intron 65.0%
GTGGTGGTTGGAGCAGCCAC+AGG + Chr8:66752212-66752231 MsG0880045944.01.T01:intron 65.0%
TCCAGCTGCTGCACCTGCTC+CGG - Chr8:66752064-66752083 MsG0880045943.01.T01:intron 65.0%
TGGAGCTGGAACCGCTGGTG+TGG + Chr8:66752231-66752250 MsG0880045944.01.T01:intron 65.0%
TTCGCGGTAGAAGCGGGTCC+AGG + Chr8:66752371-66752390 MsG0880045944.01.T01:intron 65.0%
! ATTATCGTCGCCGGCGTCGG+AGG - Chr8:66751906-66751925 MsG0880045943.01.T01:three_prime_UTR 65.0%
! GAGGTGGTTGGTGCAGCGGA+AGG + Chr8:66751939-66751958 MsG0880045944.01.T01:intron 65.0%
! GCTGCCGGAGGTTTGGTTGC+CGG + Chr8:66752086-66752105 MsG0880045944.01.T01:intron 65.0%
! GCTGGTGGACCCAACAACGC+TGG + Chr8:66752305-66752324 MsG0880045944.01.T01:intron 65.0%
! GGAGGTTTGGTTGCCGGAGC+AGG + Chr8:66752080-66752099 MsG0880045944.01.T01:intron 65.0%
!! CCCTTGCTTGGTGCTGGAGC+TGG + Chr8:66752245-66752264 MsG0880045944.01.T01:intron 65.0%
!! GCTGGTGGTGGAGTGCTGAC+TGG + Chr8:66752128-66752147 MsG0880045944.01.T01:intron 65.0%
!! GGCCGGAGTTGATGGTGAGG+TGG + Chr8:66751955-66751974 MsG0880045944.01.T01:intron 65.0%
!! GTGCTGACTGGTGTCACCGC+CGG + Chr8:66752116-66752135 MsG0880045944.01.T01:intron 65.0%
CCACGCGCCACCATTGACGG+TGG - Chr8:66752553-66752572 MsG0880045943.01.T01:intron 70.0%
GGAGCAGGTGCAGCAGCTGG+AGG + Chr8:66752065-66752084 MsG0880045944.01.T01:intron 70.0%
GGTCCACCAGCACCACCAGC+AGG - Chr8:66752314-66752333 MsG0880045943.01.T01:intron 70.0%
GGTCCAGGTGCTCCTGCTGG+TGG + Chr8:66752329-66752348 MsG0880045944.01.T01:intron 70.0%
TCCGGCAGCATCACCACCGG+CGG - Chr8:66752097-66752116 MsG0880045943.01.T01:intron 70.0%
! ACCGCCGGTGGTGATGCTGC+CGG + Chr8:66752101-66752120 MsG0880045944.01.T01:intron 70.0%
! CCACCGTCAATGGTGGCGCG+TGG + Chr8:66752556-66752575 MsG0880045944.01.T01:intron 70.0%
! GCTCCTGCTGGTGGTGCTGG+TGG + Chr8:66752320-66752339 MsG0880045944.01.T01:intron 70.0%
! GGTGCTCCTGCTGGTGGTGC+TGG + Chr8:66752323-66752342 MsG0880045944.01.T01:intron 70.0%
! GGTGCTGGAGCTGGAACCGC+TGG + Chr8:66752236-66752255 MsG0880045944.01.T01:intron 70.0%
!! AGGTGACTGGCCTCCGACGC+CGG + Chr8:66751919-66751938 MsG0880045944.01.T01:intron 70.0%
!! CTGACTGGTGTCACCGCCGG+TGG + Chr8:66752113-66752132 MsG0880045944.01.T01:intron 70.0%
!! GCAACAGGAGTGGTGGCGGC+CGG + Chr8:66751972-66751991 MsG0880045944.01.T01:intron 70.0%
!! GCACCACCAGCAGGAGCACC+TGG - Chr8:66752323-66752342 MsG0880045943.01.T01:intron 70.0%
!! GGCGGCCGGAGTTGATGGTG+AGG + Chr8:66751958-66751977 MsG0880045944.01.T01:intron 70.0%
!! GTGGTGGCGGCCGGAGTTGA+TGG + Chr8:66751963-66751982 MsG0880045944.01.T01:intron 70.0%
GCCGGAGCAGGTGCAGCAGC+TGG + Chr8:66752068-66752087 MsG0880045944.01.T01:intron 75.0%
! GCCGGTGGTGATGCTGCCGG+AGG + Chr8:66752098-66752117 MsG0880045944.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 66751786 66753532 66751786 ID=MsG0880045943.01;Name=MsG0880045943.01
Chr8 mRNA 66751786 66753532 66751786 ID=MsG0880045943.01.T01;Parent=MsG0880045943.01;Name=MsG0880045943.01.T01;_AED=0.46;_eAED=0.46;_QI=81|1|1|1|0|0|2|210|203
Chr8 exon 66751786 66752076 66751786 ID=MsG0880045943.01.T01:exon:13870;Parent=MsG0880045943.01.T01
Chr8 exon 66752921 66753532 66752921 ID=MsG0880045943.01.T01:exon:13869;Parent=MsG0880045943.01.T01
Chr8 five_prime_UTR 66753452 66753532 66753452 ID=MsG0880045943.01.T01:five_prime_utr;Parent=MsG0880045943.01.T01
Chr8 CDS 66752921 66753451 66752921 ID=MsG0880045943.01.T01:cds;Parent=MsG0880045943.01.T01
Chr8 CDS 66751996 66752076 66751996 ID=MsG0880045943.01.T01:cds;Parent=MsG0880045943.01.T01
Chr8 three_prime_UTR 66751786 66751995 66751786 ID=MsG0880045943.01.T01:three_prime_utr;Parent=MsG0880045943.01.T01
Chr8 mRNA 66752873 66753451 66752873 ID=MsG0880045943.01.T02;Parent=MsG0880045943.01;Name=MsG0880045943.01.T02;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|192
Chr8 exon 66752873 66753451 66752873 ID=MsG0880045943.01.T02:exon:13871;Parent=MsG0880045943.01.T02
Chr8 CDS 66752873 66753451 66752873 ID=MsG0880045943.01.T02:cds;Parent=MsG0880045943.01.T02
Gene Sequence

>MsG0880045943.01.T01

ATGGATCGTAACAATCTCTTCACCCTCGCATTCATCTGCATTATCGTCGCCGGCGTCGGAGGCCAGTCACCTTCCGCTGCACCAACCACCTCACCATCAACTCCGGCCGCCACCACTCCTGTTGCATCTCCGGTAGCAGCACCTCCTACAACCACACCAACAACACCAGCTCCCGTTGCTTCTCCCAAATCATCTCCTCCAGCTGCTGCACCTGCTCCGGCAACCAAACCTCCGGCAGCATCACCACCGGCGGTGACACCAGTCAGCACTCCACCACCAGCACCAGTTCCTGTCAAATCACCACCCACACCTGCTCCAGTCAGCTCACCACCAGCAGTAACACCTGTGGCTGCTCCAACCACCACACCAGCGGTTCCAGCTCCAGCACCAAGCAAGGGAAAGAAGAACAAGAAGCACGGTGCTCCAGCACCATCACCAGCGTTGTTGGGTCCACCAGCACCACCAGCAGGAGCACCTGGACCAAGCGAAGATGCAGGAGCACCTGGACCCGCTTCTACCGCGAACGATGAGAGTGGAGCAGAAACTATCAGGAGCTTGAAGATGCTAGGAGGCTTAGCTTTGAGCTGGATGGCTGTTGTTTTATTCTTCTAG

>MsG0880045943.01.T02

ATGGATCGTAACAATCTCTTCACCCTCGCATTCATCTGCATTATCGTCGCCGGCGTCGGAGGCCAGTCACCTTCCGCTGCACCAACCACCTCACCATCAACTCCGGCCGCCACCACTCCTGTTGCATCTCCGGTAGCAGCACCTCCTACAACCACACCAACAACACCAGCTCCCGTTGCTTCTCCCAAATCATCTCCTCCAGCTGCTGCACCTGCTCCGGCAACCAAACCTCCGGCAGCATCACCACCGGCGGTGACACCAGTCAGCACTCCACCACCAGCACCAGTTCCTGTCAAATCACCACCCACACCTGCTCCAGTCAGCTCACCACCAGCAGTAACACCTGTGGCTGCTCCAACCACCACACCAGCGGTTCCAGCTCCAGCACCAAGCAAGGGAAAGAAGAACAAGAAGCACGGTGCTCCAGCACCATCACCAGCGTTGTTGGGTCCACCAGCACCACCAGCAGGAGCACCTGGACCAAGCGAAGATGCAGGAGCACCTGGACCCGCTTCTACCGCGAACGATGAGGTAAATCTTGTTCATCTTTTTTTTGTCTTTTCTTTGTCGTTTCTATAG
Protein sequence

>MsG0880045943.01.T01

MDRNNLFTLAFICIIVAGVGGQSPSAAPTTSPSTPAATTPVASPVAAPPTTTPTTPAPVASPKSSPPAAAPAPATKPPAASPPAVTPVSTPPPAPVPVKSPPTPAPVSSPPAVTPVAAPTTTPAVPAPAPSKGKKNKKHGAPAPSPALLGPPAPPAGAPGPSEDAGAPGPASTANDESGAETIRSLKMLGGLALSWMAVVLFF*

>MsG0880045943.01.T02

MDRNNLFTLAFICIIVAGVGGQSPSAAPTTSPSTPAATTPVASPVAAPPTTTPTTPAPVASPKSSPPAAAPAPATKPPAASPPAVTPVSTPPPAPVPVKSPPTPAPVSSPPAVTPVAAPTTTPAVPAPAPSKGKKNKKHGAPAPSPALLGPPAPPAGAPGPSEDAGAPGPASTANDEVNLVHLFFVFSLSFL*