Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046964.01.T01 | KEH32255.1 | 95.312 | 64 | 2 | 1 | 2 | 65 | 277 | 339 | 8.94E-31 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046964.01.T01 | A0A072V2C0 | 95.312 | 64 | 2 | 1 | 2 | 65 | 277 | 339 | 4.27e-31 | 120 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045145.01 | MsG0880046964.01 | 0.810133 | 1.341140e-50 | 8.019666e-48 |
| MsG0880045965.01 | MsG0880046964.01 | 0.807502 | 4.898826e-50 | 2.733174e-47 |
| MsG0880046964.01 | MsG0880047472.01 | 0.815985 | 6.982873e-52 | 4.887050e-49 |
| MsG0180004050.01 | MsG0880046964.01 | 0.811522 | 6.711944e-51 | 4.164063e-48 |
| MsG0180004481.01 | MsG0880046964.01 | 0.800136 | 1.660705e-48 | 7.673492e-46 |
| MsG0780040545.01 | MsG0880046964.01 | 0.800464 | 1.423578e-48 | 6.632743e-46 |
| MsG0780041796.01 | MsG0880046964.01 | 0.810626 | 1.049994e-50 | 6.360968e-48 |
| MsG0680031507.01 | MsG0880046964.01 | 0.821958 | 3.060854e-53 | 2.526941e-50 |
| MsG0480021452.01 | MsG0880046964.01 | 0.820468 | 6.752638e-53 | 5.347547e-50 |
| MsG0480022696.01 | MsG0880046964.01 | 0.827284 | 1.702886e-54 | 1.636686e-51 |
| MsG0380016860.01 | MsG0880046964.01 | 0.805250 | 1.461500e-49 | 7.694551e-47 |
| MsG0780037240.01 | MsG0880046964.01 | 0.803236 | 3.837736e-49 | 1.918555e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046964.01.T01 | MTR_4g117990 | 95.312 | 64 | 2 | 1 | 2 | 65 | 277 | 339 | 1.08e-34 | 120 |
| MsG0880046964.01.T01 | MTR_7g081190 | 95.000 | 40 | 2 | 0 | 4 | 43 | 309 | 348 | 3.40e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046964.01.T01 | AT2G44730 | 76.190 | 42 | 10 | 0 | 2 | 43 | 317 | 358 | 5.22e-15 | 67.4 |
Find 15 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTCTCTTATTGAT+AGG | 0.313357 | 8:-80413826 | None:intergenic |
| CATTATGATTGTTGTGGTGA+TGG | 0.489229 | 8:-80413844 | None:intergenic |
| GCTGTCATCGGAAACTGCCT+TGG | 0.529999 | 8:-80413776 | None:intergenic |
| AAAAGAAGATGGAGATGGCG+AGG | 0.533281 | 8:+80413668 | MsG0880046964.01.T01:CDS |
| ACTGTTGTTATTGCTGTCAT+CGG | 0.538503 | 8:-80413788 | None:intergenic |
| TGAAACTATGAGGATGGAGA+TGG | 0.538920 | 8:+80413696 | MsG0880046964.01.T01:CDS |
| AAAGAAGATGGAGATGGCGA+GGG | 0.546801 | 8:+80413669 | MsG0880046964.01.T01:CDS |
| CGAGGGAGATTGAAACTATG+AGG | 0.548584 | 8:+80413686 | MsG0880046964.01.T01:CDS |
| TTTGTGAATGGAAAAGAAGA+TGG | 0.549795 | 8:+80413657 | None:intergenic |
| TGAATCTCAACAGCGAATTG+TGG | 0.554265 | 8:+80413744 | MsG0880046964.01.T01:CDS |
| GGAGATTGAAACTATGAGGA+TGG | 0.585982 | 8:+80413690 | MsG0880046964.01.T01:CDS |
| GTCGAACATTATGATTGTTG+TGG | 0.614285 | 8:-80413850 | None:intergenic |
| ATCTCAACAGCGAATTGTGG+AGG | 0.620844 | 8:+80413747 | MsG0880046964.01.T01:CDS |
| AATTGTGGAGGCATTCGCCA+AGG | 0.638281 | 8:+80413759 | MsG0880046964.01.T01:CDS |
| AATGGAAAAGAAGATGGAGA+TGG | 0.684189 | 8:+80413663 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATGGAATTCTCTTATTGAT+AGG | - | Chr8:80413829-80413848 | None:intergenic | 30.0% | |
| ! | ACTGTTGTTATTGCTGTCAT+CGG | - | Chr8:80413791-80413810 | None:intergenic | 35.0% |
| GGAGATTGAAACTATGAGGA+TGG | + | Chr8:80413690-80413709 | MsG0880046964.01.T01:CDS | 40.0% | |
| TGAAACTATGAGGATGGAGA+TGG | + | Chr8:80413696-80413715 | MsG0880046964.01.T01:CDS | 40.0% | |
| TGAATCTCAACAGCGAATTG+TGG | + | Chr8:80413744-80413763 | MsG0880046964.01.T01:CDS | 40.0% | |
| AAAAGAAGATGGAGATGGCG+AGG | + | Chr8:80413668-80413687 | MsG0880046964.01.T01:CDS | 45.0% | |
| AAAGAAGATGGAGATGGCGA+GGG | + | Chr8:80413669-80413688 | MsG0880046964.01.T01:CDS | 45.0% | |
| ATCTCAACAGCGAATTGTGG+AGG | + | Chr8:80413747-80413766 | MsG0880046964.01.T01:CDS | 45.0% | |
| CGAGGGAGATTGAAACTATG+AGG | + | Chr8:80413686-80413705 | MsG0880046964.01.T01:CDS | 45.0% | |
| !! | AATTGTGGAGGCATTCGCCA+AGG | + | Chr8:80413759-80413778 | MsG0880046964.01.T01:CDS | 50.0% |
| GCTGTCATCGGAAACTGCCT+TGG | - | Chr8:80413779-80413798 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 80413664 | 80413864 | 80413664 | ID=MsG0880046964.01;Name=MsG0880046964.01 |
| Chr8 | mRNA | 80413664 | 80413864 | 80413664 | ID=MsG0880046964.01.T01;Parent=MsG0880046964.01;Name=MsG0880046964.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|66 |
| Chr8 | exon | 80413664 | 80413864 | 80413664 | ID=MsG0880046964.01.T01:exon:10316;Parent=MsG0880046964.01.T01 |
| Chr8 | CDS | 80413664 | 80413864 | 80413664 | ID=MsG0880046964.01.T01:cds;Parent=MsG0880046964.01.T01 |
| Gene Sequence |
| Protein sequence |
